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https://www.readbyqxmd.com/read/28918151/internal-transcribed-spacer-its-sequencing-reveals-considerable-fungal-diversity-in-dairy-products
#1
A J Buehler, R L Evanowski, N H Martin, K J Boor, M Wiedmann
Fungi are important spoilage organisms in dairy products. However, little is known about the diversity of naturally occurring spoilage fungi in raw milk and processed dairy products, due at least in part to the fact that classical fungal identification methods require considerable expertise. To gain further insight into the fungal diversity in the dairy system, we isolated fungi from raw milk, raw and pasteurized milk cheese, and yogurt using the selective dichloran rose bengal chloramphenicol agar. In total, 361 fungal isolates were obtained and further characterized by DNA sequencing of the internal transcribed spacer (ITS) region and the nuclear ribosomal large subunit (LSU) rRNA gene if needed...
September 13, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28917978/sen1500-a-novel-oral-amyloid-%C3%AE-aggregation-inhibitor-attenuates-brain-pathology-in-a-mouse-model-of-alzheimer-s-disease
#2
D Brunner, S Flunkert, J Neddens, S Duller, D I C Scopes, J M Treherne, B Hutter-Paier
INTRODUCTION: Amyloid-β (Aβ) aggregation is thought to be a major pathogenic event underlying the neuropathology of Alzheimer's disease (AD). The development of new drugs inhibiting the Aβ aggregation process is, therefore, important. SEN1500, an orally bioavailable and CNS-penetrant Aβ aggregation inhibitor, has previously been shown to reduce spatial learning and memory deficits in an APP transgenic mouse model. To verify that the pharmacological properties of SEN1500 are not unique to this model, we investigated brain Aβ pathology, neuroinflammation, as well as memory in a different mouse model of AD expressing the human amyloid precursor protein with Swedish and London mutations (APPSL)...
September 13, 2017: Neuroscience Letters
https://www.readbyqxmd.com/read/28917879/laccase-tempo-oxidation-in-the-production-of-mechanically-strong-arabinoxylan-and-glucomannan-aerogels
#3
Kirsti Parikka, Ida Nikkilä, Leena Pitkänen, Abdul Ghafar, Tuula Sontag-Strohm, Maija Tenkanen
New wheat arabinoxylan and konjac glucomannan hydrogels and aerogels were prepared by hemiacetal crosslinking induced by laccase/TEMPO (2,2,6,6-tetramethylpiperidine-1-oxyl) -catalysed oxidation, which selectively converts the primary hydroxyl groups to aldehydes. The degree of oxidation of the product aldehydes was ca. 10% of the total carbohydrates of the polysaccharides, and the determination of storage and viscous moduli of the oxidised samples showed that they had formed true hydrogels. Two freezing methods for the hydrogels, conventional freezing and ice crystal templating, were investigated for aerogel production, the ice crystal templated products especially were mechanically strong in compression test against the ice crystals' growth direction...
November 1, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28917797/a-rapid-and-specific-colorimetric-method-for-free-tryptophan-quantification
#4
Yinan Wu, Tianmin Wang, Chong Zhang, Xin-Hui Xing
Tryptophan is one of the eight essential amino acids and plays an important role in many biological processes. For its interaction with human health, environment and relevant commercial interest in biotechnology-based production, rapid and specific quantification method for this molecule accessible to common laboratories is badly needed. We herein reported a simple colorimetric method for free tryptophan quantification with 96-well-plate-level throughput. Our protocol firstly converted tryptophan to indole enzymatically by purified tryptophanases and then used reactivity of indole with hydroxylamine to form pink product with absorption peak at 530nm, enabling the quantification of tryptophan with simple spectrometry in just two hours...
January 1, 2018: Talanta
https://www.readbyqxmd.com/read/28917783/intellectual-modifying-a-bare-glassy-carbon-electrode-to-fabricate-a-novel-and-ultrasensitive-electrochemical-biosensor-application-to-determination-of-acrylamide-in-food-samples
#5
Kambiz Varmira, Omid Abdi, Mohammad-Bagher Gholivand, Hector C Goicoechea, Ali R Jalalvand
Acrylamide (AA) is a neurotoxin and carcinogen which is mainly formed in foods containing large quantities of starch processed at high temperatures and its determination is very important to control the quality of foods. In this work, a novel electrochemical biosensor based on hemoglobin-dimethyldioctadecylammonium bromide (HG-DDAB)/platinum-gold-palladium three metallic alloy nanoparticles (PtAuPd NPs)/chitosan-1-ethyl-3-methylimidazolium bis(trifluoromethylsulfonyl)imide (Ch-IL)/multiwalled carbon nanotubes-IL (MWCNTs-IL)/glassy carbon electrode (GCE) is proposed for ultrasensitive determination of AA in food samples...
January 1, 2018: Talanta
https://www.readbyqxmd.com/read/28917435/measurement-of-micronuclei-and-internal-dose-in-mice-demonstrates-that-3-monochloropropane-1-2-diol-3-mcpd-has-no-genotoxic-potency-in-vivo
#6
Jenny Aasa, Margareta Törnqvist, Lilianne Abramsson-Zetterberg
In this study 3-monochloropropane-1,2-diol (3-MCPD), a compound that appears as contaminant in refined cooking oils, has been studied with regard to genotoxicity in vivo (mice) with simultaneous measurement of internal dose using state-of-the-art methodologies. Genotoxicity (chromosomal aberrations) was measured by flow cytometry with dual lasers as the frequency of micronuclei in erythrocytes in peripheral blood from BalbC mice intraperitoneally exposed to 3-MCPD (0, 50, 75, 100, 125 mg/kg). The internal doses of 3-MCPD in the mice were calculated from N-(2,3-dihydroxypropyl)-valine adducts to hemoglobin (Hb), quantified at very low levels by high-resolution mass spectrometry...
September 13, 2017: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/28917244/dietary-patterns-and-sleep-parameters-in-a-cohort-of-community-dwelling-australian-men
#7
Yingting Cao, Anne W Taylor, Gary Wittert, Robert Adams, Zumin Shi
BACKGROUND AND OBJECTIVES: Emerging evidence suggests potential effects of nutrients/foods on sleep parameters. However, no studies have addressed the complex interactions among nutrients/foods and relate them to sleep outcomes. To investigate the associations between dietary patterns and sleep parameters (polysomnography (PSG) measured and self-reported sleep symptoms) in a large sample of community dwelling men in South Australia. METHODS AND STUDY DESIGN: Cross-sectional analysis was conducted of participants in the Men Androgen Inflammation Lifestyle Environment and Stress cohort enrolled in a sleep sub-study (n=784, age 35-80 years)...
2017: Asia Pacific Journal of Clinical Nutrition
https://www.readbyqxmd.com/read/28917241/dietary-sodium-reduction-in-new-zealand-influence-of-the-tick-label
#8
Sherry X Ning, Louise A Mainvil, Rachel K Thomson, Rachel M McLean
BACKGROUND AND OBJECTIVES: The Tick programme of the National Heart Foundation (NHF) is the longest standing voluntary front of pack signpost nutrition logo in New Zealand. It provides a platform for collaboration with the food industry to encourage development of healthier products. This study evaluated the impact of the Tick programme on sodium in processed food. METHODS AND STUDY DESIGN: Fifty-two Tick programme products from food categories known to contribute substantially to sodium intake were identified...
2017: Asia Pacific Journal of Clinical Nutrition
https://www.readbyqxmd.com/read/28917225/appropriate-nutritional-management-in-patients-with-impaired-mastication-and-those-with-mild-dysphagia-a-multicenter-study-of-the-usefulness-of-novel-foods-processed-and-softened-by-enzymes
#9
Takashi Higashiguchi, Akihiro Ito, Hirofumi Nishiyama, Takashi Shigematsu, Atsuko Ishikawa, Hiroyuki Kato, Shohei Iijima, Nobuyuki Kikuchi
BACKGROUND AND OBJECTIVES: Our aim was to investigate the safety of iEAT (a food that is softened by heat and enzyme homogeneous permeation) and iEAT-affected nutrition parameters, e.g., nutrition intake (calculated from the consumption rate in patients with impaired mastication and those with mild dysphagia). METHODS AND STUDY DESIGN: A multicenter, randomized, cross-over study of iEAT was conducted in 50 patients (mean age 77.0±11.0 years) with dysphagia due to Occasional aspiration (4 points on the Dysphagia Severity Scale [DSS]) or Oral problems (5 points) randomly assigned to the study diet (iEAT) or its opposite (the modified traditional [control] diet) for 1 week and then switched for 1 week to the opposing diet...
2017: Asia Pacific Journal of Clinical Nutrition
https://www.readbyqxmd.com/read/28917104/bacteria-and-archaea-communities-in-full-scale-thermophilic-and-mesophilic-anaerobic-digesters-treating-food-wastewater-key-process-parameters-and-microbial-indicators-of-process-instability
#10
Joonyeob Lee, Seung Gu Shin, Gyuseong Han, Taewoan Koo, Seokhwan Hwang
In this study, four different mesophilic and thermophilic full-scale anaerobic digesters treating food wastewater (FWW) were monitored for 1-2years in order to investigate: 1) microbial communities underpinning anaerobic digestion of FWW, 2) significant factors shaping microbial community structures, and 3) potential microbial indicators of process instability. Twenty-seven bacterial genera were identified as abundant bacteria underpinning the anaerobic digestion of FWW. Methanosaeta harundinacea, M. concilii, Methanoculleus bourgensis, M...
September 5, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28916914/the-effects-of-protein-and-fiber-content-on-gut-structure-and-function-in-zebrafish-danio-rerio
#11
Samantha C Leigh, Bao-Quang Nguyen-Phuc, Donovan P German
Chemical reactor theory (CRT) suggests that the digestive tract functions as a chemical reactor for processing food. Presumably, gut structure and function should match diet to ensure adequate nutrient and energy uptake to maintain performance. Within CRT, dietary biochemical composition is the most important factor affecting gut structure and function in vertebrates. We fed Danio rerio (zebrafish) diets ranging from high- to moderate- to low-quality (i.e., ranging from high-protein, low-fiber to low-protein, high-fiber), and observed how gut length and surface area, as well as the activity levels of digestive enzymes (amylase, maltase, trypsin, aminopeptidase, and lipase) shifted in response to these dietary changes...
September 15, 2017: Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology
https://www.readbyqxmd.com/read/28915964/decreased-use-of-spatial-pattern-separation-in-contemporary-lifestyles-may-contribute-to-hippocampal-atrophy-and-diminish-mental-health
#12
Lindy Birkel
The spatial pattern separation process has not yet been proposed as a pivotal neural activity affecting hippocampal volume, a metric of mental health. The dentate gyrus/CA3 region is increasingly implicated in hippocampal atrophy, its putative role in spatial pattern separation appears to be impaired in mental health disorders, and performance on a task indicative of pattern separation correlates with dentate gyrus/CA3 volume. Spatial pattern separation is thought to utilize the heightened neural plasticity of newborn neurons in the dentate gyrus to distinguish highly similar aspects of scene so that these remain distinct in memory rather than lost...
September 2017: Medical Hypotheses
https://www.readbyqxmd.com/read/28915825/effect-of-a-mobile-app-intervention-on-vegetable-consumption-in-overweight-adults-a-randomized-controlled-trial
#13
Sarah Mummah, Thomas N Robinson, Maya Mathur, Sarah Farzinkhou, Stephen Sutton, Christopher D Gardner
BACKGROUND: Mobile applications (apps) have been heralded as transformative tools to deliver behavioral health interventions at scale, but few have been tested in rigorous randomized controlled trials. We tested the effect of a mobile app to increase vegetable consumption among overweight adults attempting weight loss maintenance. METHODS: Overweight adults (n=135) aged 18-50 years with BMI=28-40 kg/m(2) near Stanford, CA were recruited from an ongoing 12-month weight loss trial (parent trial) and randomly assigned to either the stand-alone, theory-based Vegethon mobile app (enabling goal setting, self-monitoring, and feedback and using "process motivators" including fun, surprise, choice, control, social comparison, and competition) or a wait-listed control condition...
September 15, 2017: International Journal of Behavioral Nutrition and Physical Activity
https://www.readbyqxmd.com/read/28914786/baseline-and-estimated-trends-of-sodium-availability-and-food-sources-in-the-costa-rican-population-during-2004-2005-and-2012-2013
#14
Adriana Blanco-Metzler, Rafael Moreira Claro, Katrina Heredia-Blonval, Ivannia Caravaca Rodríguez, María de Los A Montero-Campos, Branka Legetic, Mary R L'Abbe
In 2012, Costa Rica launched a program to reduce salt and sodium consumption to prevent cardiovascular disease and associated risk factors, but little was known about the level of sodium consumption or its sources. Our aim was to estimate the magnitude and time trends of sodium consumption (based on food and beverage acquisitions) in Costa Rica. Data from the National Household Income and Expenditure Surveys carried out in 2004-2005 (n = 4231) and 2012-2013 (n = 5705) were used. Records of food purchases for household consumption were converted into sodium and energy using food composition tables...
September 15, 2017: Nutrients
https://www.readbyqxmd.com/read/28914589/hplc-dad-method-development-and-validation-for-the-quantification-of-hydroxymethylfurfural-in-corn-chips-by-means-of-response-surface-optimization
#15
Sara Salvatierra Virgen, Silvia Guillermina Ceballos-Magaña, Vilma Del Carmen Salvatierra-Stamp, Maria Teresa Sumaya-Martínez, Francisco Javier Martínez-Martínez, Roberto Muñiz-Valencia
In recent years, there has been an increased concern about the presence of toxic compounds derived from the Maillard reaction produced during food cooking at high temperatures. The main toxic compounds derived from this reaction are acrylamide and hydroxymethylfurfural (HMF). The majority of analytical methods require sample treatments using solvents which are highly polluting for the environment. The difficulty for quantifying HMF in complex fried food matrices encourages the development of new analytical methods...
September 15, 2017: Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment
https://www.readbyqxmd.com/read/28914443/indirect-effects-of-larvae-dispersal-following-mass-mortality-events
#16
Marcus A Lashley, Heather R Jordan, Jeffery K Tomberlin, Brandon T Barton
Mass mortality events are characterized by rapid die-offs of many individuals within a population at a specific location. These events produce a high concentration of remains within a given locale and the frequency and magnitude of these events may be increasing (Fey et al. 2015). Mass mortality events may be caused by physical (e.g., lightning strikes, fire), chemical (e.g., pollutants, hypoxia), or biological processes (e.g., disease, phenological mismatch with food source). This article is protected by copyright...
September 15, 2017: Ecology
https://www.readbyqxmd.com/read/28914314/the-impact-of-extrusion-on-the-nutritional-composition-dietary-fiber-and-in-vitro-digestibility-of-gluten-free-snacks-based-on-rice-pea-and-carob-flour-blends
#17
C Arribas, B Cabellos, C Sánchez, C Cuadrado, E Guillamón, M M Pedrosa
Consumers and the food industry are demanding healthier products. Expanded snacks with a high nutritional value were developed from different rice, pea and carob flour blends. The proximate composition, starch (total and resistant), amylose and amylopectin, dietary fiber (soluble and insoluble) contents, and the in vitro protein digestibility of different rice-legume formulations, were evaluated before and after the extrusion process. Compared with the corresponding non-extruded blends (control), the extrusion treatment did not change the total protein content, however, it reduced the soluble protein (61-86%), the fat (69-92%) and the resistant starch contents (100%)...
September 15, 2017: Food & Function
https://www.readbyqxmd.com/read/28914313/cohesiveness-and-flowability-of-particulated-solid-and-semi-solid-food-systems
#18
Aarti B Tobin, Peggy Heunemann, Judith Wemmer, Jason R Stokes, Timothy Nicholson, Erich J Windhab, Peter Fischer
Cohesiveness and flowability of particulated food systems is of particular interest in the oral processing and swallowing of food products, especially for people suffering from dysphagia. Although cohesiveness of a bolus is an essential parameter in swallowing, a robust technique for objective measurement of cohesiveness of particulated semi- or soft-solids is still lacking. In our approach the ring shear tester is used to measure the cohesiveness and flowability of a model particulated food system based on fresh green pea powders and pastes with controlled moisture content...
September 15, 2017: Food & Function
https://www.readbyqxmd.com/read/28913736/ptfe-coated-non-stick-cookware-and-toxicity-concerns-a-perspective
#19
Muhammad Sajid, Muhammad Ilyas
PTFE is used as an inner coating material in non-stick cookware. This unique polymer coating prevents food from sticking in the pans during the cooking process. Such cookware is also easy to wash. At normal cooking temperatures, PTFE-coated cookware releases various gases and chemicals that present mild to severe toxicity. Only few studies describe the toxicity of PTFE but without solid conclusions. The toxicity and fate of ingested PTFE coatings are also not understood. Moreover, the emerging, persistent, and well-known toxic environmental pollutant PFOA is also used in the synthesis of PTFA...
September 14, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28912815/feasibility-of-metatranscriptome-analysis-from-infant-gut-microbiota-adaptation-to-solid-foods-results-in-increased-activity-of-firmicutes-at-six-months
#20
Floor Hugenholtz, Jarmo Ritari, Lotta Nylund, Mark Davids, Reetta Satokari, Willem M de Vos
Newborns are rapidly colonized by microbes and their intestinal tracts contain highly dynamic and rapidly developing microbial communities in the first months of life. In this study, we describe the feasibility of isolating mRNA from rapidly processed faecal samples and applying deep RNA-Seq analysis to provide insight into the active contributors of the microbial community in early life. Specific attention is given to the impact of removing rRNA from the mRNA on the phylogenetic and transcriptional profiling and its analysis depth...
2017: International Journal of Microbiology
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