Yuchen Wang, Yanna Liu, Wenfeng Deng, Fangxiang Fu, Susha Yan, Hongwei Yang, Rumin Liu, Jian Geng, Jian Xu, Yihan Wu, Junfeng Ma, Jiapeng Zhou, Na Liu, Yu Jin, Renfei Xia, Nahel Elias, Richard J Lee, Adam S Feldman, Michael L Blute, Robert B Colvin, Chin-Lee Wu, Yun Miao
BK polyomavirus (BKPyV)-associated cancer after transplantation has gained increasing attention. However, the role of BKPyV integration on oncogenesis is still unclear. In this study, next-generation virome capture sequencing of primary and metastatic tumors were performed in three patients with BKPyV-associated urothelial carcinoma after renal transplantation. As a result, a total of 332 viral integration sites were identified in the six tumors. Integration of BKPyV in both primary and metastatic tumors followed the mechanism of microhomology-mediated end joining mostly, since microhomologies between human and BKPyV genomes were significantly enriched in flanking regions of 84% of the integration sites...
August 2020: Oncogene