keyword
MENU ▼
Read by QxMD icon Read
search

clostridium difficile 027

keyword
https://www.readbyqxmd.com/read/28814976/analysis-of-proteomes-released-from-in-vitro-cultured-eight-clostridium-difficile-pcr-ribotypes-revealed-specific-expression-in-pcr-ribotypes-027-and-176-confirming-their-genetic-relatedness-and-clinical-importance-at-the-proteomic-level
#1
Jiri Dresler, Marcela Krutova, Alena Fucikova, Jana Klimentova, Veronika Hruzova, Miloslava Duracova, Katerina Houdkova, Barbora Salovska, Jana Matejkova, Martin Hubalek, Petr Pajer, Libor Pisa, Otakar Nyc
BACKGROUND: Clostridium difficile is the causative agent of C. difficile infection (CDI) that could be manifested by diarrhea, pseudomembranous colitis or life-threatening toxic megacolon. The spread of certain strains represents a significant economic burden for health-care. The epidemic successful strains are also associated with severe clinical features of CDI. Therefore, a proteomic study has been conducted that comprises proteomes released from in vitro cultured panel of eight different PCR ribotypes (RTs) and employs the combination of shotgun proteomics and label-free quantification (LFQ) approach...
2017: Gut Pathogens
https://www.readbyqxmd.com/read/28813461/epidemiology-of-clostridium-difficile-in-infants-in-oxfordshire-uk-risk-factors-for-colonization-and-carriage-and-genetic-overlap-with-regional-c-difficile-infection-strains
#2
Nicole Stoesser, David W Eyre, T Phuong Quan, Heather Godwin, Gemma Pill, Emily Mbuvi, Alison Vaughan, David Griffiths, Jessica Martin, Warren Fawley, Kate E Dingle, Sarah Oakley, Kazimierz Wanelik, John M Finney, Melina Kachrimanidou, Catrin E Moore, Sherwood Gorbach, Thomas V Riley, Derrick W Crook, Tim E A Peto, Mark H Wilcox, A Sarah Walker
BACKGROUND: Approximately 30-40% of children <1 year of age are Clostridium difficile colonized, and may represent a reservoir for adult C. difficile infections (CDI). Risk factors for colonization with toxigenic versus non-toxigenic C. difficile strains and longitudinal acquisition dynamics in infants remain incompletely characterized. METHODS: Predominantly healthy infants (≤2 years) were recruited in Oxfordshire, UK, and provided ≥1 fecal samples. Independent risk factors for toxigenic/non-toxigenic C...
2017: PloS One
https://www.readbyqxmd.com/read/28807623/spatio-temporal-variability-of-the-epidemic-027-clostridium-difficile-strains-in-france-based-on-mlva-typing
#3
Jeanne Couturier, Catherine Eckert, Frédéric Barbut
MLVA analysis of 103 PCR ribotype 027 strains showed a regional specificity and the persistence of the same clone within a hospital several years apart. Capillary electrophoresis PCR ribotyping led to the identification of seven 027 variant strains and five 176 strains, four of them being implicated in an outbreak.
August 11, 2017: Anaerobe
https://www.readbyqxmd.com/read/28794800/genome-characterization-of-a-novel-binary-toxin-positive-strain-of-clostridium-difficile-and-comparison-with-the-epidemic-027-and-078-strains
#4
Zhong Peng, Sidi Liu, Xiujuan Meng, Wan Liang, Zhuofei Xu, Biao Tang, Yuanguo Wang, Juping Duan, Chenchao Fu, Bin Wu, Anhua Wu, Chunhui Li
BACKGROUND: Clostridium difficile is an anaerobic Gram-positive spore-forming gut pathogen that causes antibiotic-associated diarrhea worldwide. A small number of C. difficile strains express the binary toxin (CDT), which is generally found in C. difficile 027 (ST1) and/or 078 (ST11) in clinic. However, we isolated a binary toxin-positive non-027, non-078 C. difficile LC693 that is associated with severe diarrhea in China. The genotype of this strain was determined as ST201. To understand the pathogenesis-basis of C...
2017: Gut Pathogens
https://www.readbyqxmd.com/read/28790208/pathogenicity-locus-core-genome-and-accessory-gene-contributions-to-clostridium-difficile-virulence
#5
Brittany B Lewis, Rebecca A Carter, Lilan Ling, Ingrid Leiner, Ying Taur, Mini Kamboj, Erik R Dubberke, Joao Xavier, Eric G Pamer
Clostridium difficile is a spore-forming anaerobic bacterium that causes colitis in patients with disrupted colonic microbiota. While some individuals are asymptomatic C. difficile carriers, symptomatic disease ranges from mild diarrhea to potentially lethal toxic megacolon. The wide disease spectrum has been attributed to the infected host's age, underlying diseases, immune status, and microbiome composition. However, strain-specific differences in C. difficile virulence have also been implicated in determining colitis severity...
August 8, 2017: MBio
https://www.readbyqxmd.com/read/28782647/host-factors-are-more-important-in-predicting-recurrent-clostridium-difficile-infection-than-ribotype-and-use-of-antibiotics
#6
Yvette H van Beurden, Shabir Nezami, Chris J J Mulder, Christina M J E Vandenbroucke-Grauls
OBJECTIVE: A frequent complication of Clostridium difficile infection (CDI) is recurrent disease. The aim of this study was to determine whether early recurrence risk was higher after infection with ribotype 027 (outbreak strain) compared to infection with endemic strain types of C. difficile METHODS: Consecutive patients diagnosed with CDI between May 2013 and March 2014 were included (outbreak strain, and non-outbreak strains). Patients who developed recurrent CDI within 30 days after completion of CDI treatment, were compared to patients without a recurrence...
August 3, 2017: Clinical Microbiology and Infection
https://www.readbyqxmd.com/read/28747822/beneficial-effect-of-bifidobacterium-longum-atcc-15707-on-survival-rate-of-clostridium-difficile-infection-in-mice
#7
Bohyun Yun, Minyu Song, Dong-June Park, Sejong Oh
Clostridium difficile infection (CDI) is the main cause of hospital-acquired diarrhea that can cause colitis or even death. The medical-treatment cost and deaths caused by CDI are increasing annually worldwide. New approaches for prevention and treatment of these infections are needed, such as the use of probiotics. Probiotics, including Bifidobacterium spp. and Lactobacillus, are microorganisms that confer a health benefit to the host when administered in adequate amounts. The effect of Bifidobacterium longum ATCC 15707 on infectious disease caused by C...
2017: Korean Journal for Food Science of Animal Resources
https://www.readbyqxmd.com/read/28729205/antimicrobial-susceptibility-of-clostridium-difficile-isolates-in-israel
#8
Linda Tkhawkho, Orna Nitzan, Nina Pastukh, Diana Brodsky, Karen Jackson, Avi Peretz
OBJECTIVES: An increase of Clostridium difficile isolates with reduced susceptibility to various antimicrobial agents has been observed, including isolates that are non-susceptible to antibiotics that are routinely used for treatment of C. difficile, such as vancomycin and metronidazole. We determined the susceptibility rates of C. difficile isolates from hospitals in northern Israel to various antibiotics including tigecycline, which was not previously reported from Israel. METHODS: A total of 81 stool samples were collected from three hospitals in northern Israel from patients with C...
July 17, 2017: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/28708379/chemical-genomics-structure-elucidation-and-in-vivo-studies-of-the-marine-derived-anticlostridial-ecteinamycin
#9
Thomas P Wyche, René F Ramos Alvarenga, Jeff S Piotrowski, Megan N Duster, Simone R Warrack, Gabriel Cornilescu, Travis J De Wolfe, Yanpeng Hou, Doug R Braun, Gregory A Ellis, Scott W Simpkins, Justin Nelson, Chad L Myers, James Steele, Hirotada Mori, Nasia Safdar, John L Markley, Scott R Rajski, Tim S Bugni
A polyether antibiotic, ecteinamycin (1), was isolated from a marine Actinomadura sp., cultivated from the ascidian Ecteinascidia turbinata. (13)C enrichment, high resolution NMR spectroscopy, and molecular modeling enabled elucidation of the structure of 1, which was validated on the basis of comparisons with its recently reported crystal structure. Importantly, ecteinamycin demonstrated potent activity against the toxigenic strain of Clostridium difficile NAP1/B1/027 (MIC = 59 ng/μL), as well as other toxigenic and nontoxigenic C...
July 26, 2017: ACS Chemical Biology
https://www.readbyqxmd.com/read/28669616/clostridium-difficile-chimeric-toxin-receptor-binding-domain-vaccine-induced-protection-against-different-strains-in-active-and-passive-challenge-models
#10
Jing-Hui Tian, Gregory Glenn, David Flyer, Bin Zhou, Ye Liu, Eddie Sullivan, Hua Wu, James F Cummings, Larry Elllingsworth, Gale Smith
Clostridium difficile is the number one cause of nosocomial antibiotic-associated diarrhea in developed countries. Historically, pathogenesis was attributed two homologous glucosylating toxins, toxin-A (TcdA) and toxin-B (TcdB). Over the past decade, however, highly virulent epidemic strains of C. difficile (B1/NAP1/027) have emerged and are linked to an increase in morbidity and mortality. Increased virulence is attributed to multiple factors including: increased production of A- and B-toxins; production of binary toxin (CDT); and the emergence of more toxic TcdB variants (TcdB(027))...
July 24, 2017: Vaccine
https://www.readbyqxmd.com/read/28641091/increasing-rates-of-clostridium-difficile-infection-in-mexican-hospitals
#11
Laura Palau Dávila, Elvira Garza-González, Patricia Rodríguez-Zulueta, Rayo Morfín-Otero, Eduardo Rodríguez-Noriega, Diana Villar-Compte, Juan C Rodríguez-Aldama, Adrián Camacho-Ortiz
INTRODUCTION: The epidemiology of Clostridium difficile infection (CDI) has changed in the last two decades. There is a lack of information regarding incidence and severity of CDI, especially in the developing world. METHODS: This was a retrospective and observational study from four hospitals of three Mexican cities. Patients were diagnosed with CDI when presented with loose stools and had at least one of the following tests positive: toxins assay, real-time PCR, or an endoscopic image compatible with pseudomembranous colitis...
June 19, 2017: Brazilian Journal of Infectious Diseases
https://www.readbyqxmd.com/read/28625250/-fulminant-clostridium-difficile-colitis-new-challenges-in-critical-care-medicine
#12
Bin Liu
Fulminant Clostridium difficile colitis (FCDC) means severe Clostridium difficile colitis (CDC), which often manifested with severe diarrhea, fever, abdominal distention, electrolyte disorder, hypoalbuminemia, and it can lead to pseudo membrane colonitis, toxic megacolon, bowel perforation, septic shock, and multiple organ dysfunction. Now Clostridium difficile (CD) becomes one of the most important medical institutions related infectious pathogens. In particular, the recently discovered mutant CD strain NAP1/BI/ribotype 027 has high pathogenicity in the global epidemic and its outbreak...
June 2017: Zhonghua Wei Zhong Bing Ji Jiu Yi Xue
https://www.readbyqxmd.com/read/28615471/clostridium-difficile-pcr-cycle-threshold-predicts-free-toxin
#13
Fiona Senchyna, Rajiv L Gaur, Saurabh Gombar, Cynthia Y Truong, Lee F Schroeder, Niaz Banaei
Background. There is no stand-alone Clostridium difficile diagnostic that can sensitively and rapidly detect fecal free toxins. We investigated the performance of C. difficile PCR cycle threshold (CT) for predicting free toxin status.Method. Consecutive stool samples (n=312) positive for toxigenic C. difficile with the GeneXpert C.diff Epi tcdB PCR assay were tested with the rapid membrane C. DIFF QUIK CHEK COMPLETE immunoassay (RMEIA). RMEIA toxin-negative samples were tested with the cell cytotoxicity neutralization assay (CCNA) and tgcBIOMICS ELISA...
June 14, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28592343/external-validation-of-three-prediction-tools-for-patients-at-risk-of-a-complicated-course-of-clostridium-difficile-infection-disappointing-in-an-outbreak-setting
#14
Yvette H van Beurden, Marjolein P M Hensgens, Olaf M Dekkers, Saskia Le Cessie, Chris J J Mulder, Christina M J E Vandenbroucke-Grauls
OBJECTIVE Estimating the risk of a complicated course of Clostridium difficile infection (CDI) might help doctors guide treatment. We aimed to validate 3 published prediction models: Hensgens (2014), Na (2015), and Welfare (2011). METHODS The validation cohort comprised 148 patients diagnosed with CDI between May 2013 and March 2014. During this period, 70 endemic cases of CDI occurred as well as 78 cases of CDI related to an outbreak of C. difficile ribotype 027. Model calibration and discrimination were assessed for the 3 prediction rules...
June 8, 2017: Infection Control and Hospital Epidemiology
https://www.readbyqxmd.com/read/28588767/effectiveness-of-various-cleaning-and-disinfectant-products-on-clostridium-difficile-spores-of-pcr-ribotypes-010-014-and-027
#15
N Kenters, E G W Huijskens, S C J de Wit, I G J M Sanders, J van Rosmalen, E J Kuijper, A Voss
BACKGROUND: In healthcare facilities, Clostridium difficile infections spread by transmission of bacterial spores. Appropriate sporicidal disinfectants are needed to prevent development of clusters and outbreaks. In this study different cleaning/disinfecting wipes and sprays were tested for their efficacy against spores of distinctive C. difficile PCR ribotypes. METHODS: Four different products were tested; 1) hydrogen peroxide 1.5%; 2) glucoprotamin 1.5%; 3) a mixture of ethanol, propane and N-alkyl amino propyl glycine; and 4) a mixture of didecyldimonium chloride, benzalkonium chloride, polyaminopropyl, biguanide and dimenthicone as active ingredients...
2017: Antimicrobial Resistance and Infection Control
https://www.readbyqxmd.com/read/28583353/the-recognition-and-characterisation-of-finnish-clostridium-difficile-isolates-resembling-pcr-ribotype-027
#16
Marcela Krutova, Otakar Nyc, Jana Matejkova, Ed J Kuijper, Jari Jalava, Silja Mentula
PURPOSE: To characterise and compare twenty-eight Finnish Clostridiumdifficile RT027-like isolates, selected based on the presence of 18 bp deletion in the tcdC gene and toxin gene profile (A, B, binary), with eleven RT027 isolates from different Finnish geographical areas and time periods. METHODS: Twenty-eight C. difficile RT027-like isolates and 11 RT027 comparative strains were characterised by capillary-electrophoresis (CE) ribotyping, multi-locus variable tandem-repeats analysis (MLVA), multi-locus sequence typing (MLST), and sequencing of tcdC and gyrA gene fragments...
May 24, 2017: Journal of Microbiology, Immunology, and Infection, Wei Mian Yu Gan Ran za Zhi
https://www.readbyqxmd.com/read/28575285/comparison-of-control-of-clostridium-difficile-infection-in-six-english-hospitals-using-whole-genome-sequencing
#17
David W Eyre, Warren N Fawley, Anu Rajgopal, Christopher Settle, Kalani Mortimer, Simon D Goldenberg, Susan Dawson, Derrick W Crook, Tim E A Peto, A Sarah Walker, Mark H Wilcox
Background.: Variation in Clostridium difficile infection (CDI) rates between healthcare institutions suggests overall incidence could be reduced if the lowest rates could be achieved more widely. Methods.: We used whole-genome sequencing (WGS) of consecutive C. difficile isolates from 6 English hospitals over 1 year (2013-14) to compare infection control performance. Fecal samples with a positive initial screen for C. difficile were sequenced. Within each hospital, we estimated the proportion of cases plausibly acquired from previous cases...
May 29, 2017: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
https://www.readbyqxmd.com/read/28555402/clostridium-difficile-infections-in-finland-2008-2015-trends-diagnostics-and-ribotypes
#18
S Mentula, S M Kotila, O Lyytikäinen, S Ibrahem, J Ollgren, A Virolainen
We evaluated Clostridium difficile (CD) diagnostics in Finnish clinical microbiology laboratories during 2006-2011, with an update in 2015, in relation to CD surveillance data of the National Infectious Disease Register (NIDR) and ribotyping data from the national reference laboratory during the years 2008-2015. In 2011, diagnostic activity varied regionally more than three-fold and the positivity rate ranged between 7 and 21%. Nucleic acid amplification testing (NAAT) was implemented in the regions with high activity and NAAT users tested 30% more patients and found 15% more cases per population than those not using it...
May 30, 2017: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/28511200/enteric-infection-in-relapse-of-inflammatory-bowel-disease-the-utility-of-stool-microbial-pcr-testing
#19
Jordan E Axelrad, Andrew Joelson, Yael R Nobel, Garrett Lawlor, Peter H R Green, Simon Lichtiger, Benjamin Lebwohl
BACKGROUND: The similar presentations in relapse of inflammatory bowel disease (IBD) and enteric infection pose substantial barriers to diagnosis and treatment. The objective of this study was to investigate the incidence, etiology, predictors, and treatment of enteric infection in patients with IBD. METHODS: We reviewed the records of 214 patients with IBD who underwent 295 gastrointestinal pathogen panel and Clostridium difficile infection (CDI) polymerase chain reaction (PCR) stool tests during an exacerbation of symptoms...
June 2017: Inflammatory Bowel Diseases
https://www.readbyqxmd.com/read/28501926/an-outbreak-of-clostridium-difficile-pcr-ribotype-027-in-spain-risk-factors-for-recurrence-and-a-novel-treatment-strategy
#20
E Bouza, L Alcalá, M Marín, M Valerio, E Reigadas, P Muñoz, M González-Del Vecchio, V de Egea
An outbreak of Clostridium difficile infection (CDI) caused by ribotype 027 (B1/NAP1) began in our hospital in November 2014, and produced 141 episodes in the following months. The aim of this study is to describe this outbreak, assess risk factors for recurrence of CDI-027 and to analyze the implementation of a novel treatment strategy. This is a prospective study of all patients with CDI-027, from November 2014 to November 2015. The epidemiological data were collected daily for each patient. We compared clinical characteristics and treatment between patients with and without recurrence of CDI-027...
May 13, 2017: European Journal of Clinical Microbiology & Infectious Diseases
keyword
keyword
85680
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"