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https://www.readbyqxmd.com/read/28774314/surfactant-effects-on-the-viability-and-function-of-human-mesenchymal-stem-cells-in-vitro-and-in-vivo-assessment
#1
Chung-Ming Chen, Hsiu-Chu Chou, Willie Lin, Chris Tseng
BACKGROUND: Surfactant therapy has become the standard of care for preterm infants with respiratory distress syndrome. Preclinical studies have reported the therapeutic benefits of mesenchymal stem cells (MSCs) in experimental bronchopulmonary dysplasia. This study investigated the effects of a surfactant on the in vitro viability and in vivo function of human MSCs. METHODS: The viability, phenotype, and mitochondrial membrane potential (MMP) of MSCs were assessed through flow cytometry...
August 3, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28753596/inhibition-of-i%C3%AE%C2%BAb%C3%AE-nf%C3%AE%C2%BAb-signaling-prevents-lps-induced-il1%C3%AE-expression-without-increasing-apoptosis-in-the-developing-mouse-lung
#2
Sarah McKenna, Brittany Butler, Laurie Jatana, Sankar Ghosh, Clyde J Wright
BACKGROUND: The pro-inflammatory consequences of IL1β expression contribute to the pathogenesis of BPD. Selectively targeting LPS-induced IκBβ/NFκB signaling attenuates IL1β mRNA expression in macrophages. Whether targeting IκBβ/NFκB signaling affects anti-apoptotic gene expression, a known consequence of global LPS-induced NFκB inhibition, is unknown. METHODS: Macrophages (RAW 264.7, BMDM) were assessed for LPS-induced IL1β mRNA/protein expression, anti-apoptotic gene expression, cell viability (trypan blue exclusion) and activation of apoptosis (caspase-3 and PARP cleavage) following pharmacologic and genetic attenuation of IκBβ/NFκB signaling...
July 28, 2017: Pediatric Research
https://www.readbyqxmd.com/read/28747684/generation-and-validation-of-novel-adeno-associated-viral-vectors-for-the-analysis-of-ca-2-homeostasis-in-motor-neurons
#3
Rosa Pia Norante, Maria Lina Massimino, Paolo Lorenzon, Agnese De Mario, Caterina Peggion, Mattia Vicario, Mattia Albiero, Maria Catia Sorgato, Raffaele Lopreiato, Alessandro Bertoli
A finely tuned Ca(2+) homeostasis in restricted cell domains is of fundamental importance for neurons, where transient Ca(2+) oscillations direct the proper coordination of electro-chemical signals and overall neuronal metabolism. Once such a precise regulation is unbalanced, however, neuronal functions and viability are severely compromised. Accordingly, disturbed Ca(2+) metabolism has often been claimed as a major contributor to different neurodegenerative disorders, such as amyotrophic lateral sclerosis that is characterised by selective motor neuron (MN) damage...
July 26, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28739338/comparative-phenotypic-analysis-of-clostridium-neonatale-and-clostridium-butyricum-isolates-from-neonates
#4
S Schönherr-Hellec, G Klein, J Delannoy, L Ferraris, I Friedel, J C Rozé, M J Butel, J Aires
"Clostridium neonatale" was recently described as a new species within the Cluster I of the Clostridium genus sensu stricto. In this study, we characterized "C. neonatale" isolates (n = 42) and compared their phenotypic properties with those of Clostridium butyricum (n = 26), a close related species. Strains isolated from fecal samples of healthy neonates were tested for different phenotypic characteristics. Compared to C. butyricum, "C. neonatale" showed a significant higher surface hydrophobicity (p = 0...
July 21, 2017: Anaerobe
https://www.readbyqxmd.com/read/28704168/integrating-agricultural-injury-prevention-with-rural-pediatrics-a-pilot-assessment
#5
Julie Kaprelian, Richard L Berg, Kathrine Lynn Barnes, Barbara Marlenga
BACKGROUND: Childhood agricultural injuries are an important public health problem. Pediatricians are a trusted source of expertise in children's health and safety and could serve as a sphere of influence to augment child agricultural injury prevention efforts. The purpose of this pilot study was to begin to explore the perspectives of pediatricians in a large rural health clinic about addressing child agricultural injury prevention within their practice. METHODS: Structured interviews were conducted with nine pediatricians who maintain a clinical practice of at least 2 days a week and care for newborns through adolescents...
July 13, 2017: Journal of Agromedicine
https://www.readbyqxmd.com/read/28668447/effect-of-cryopreservation-on-viability-and-growth-efficiency-of-stromal-epithelial-cells-derived-from-neonatal-human-thymus
#6
Valentin P Shichkin, Oleksandr I Gorbach, Olga A Zuieva, Nataliia I Grabchenko, Irina A Aksyonova, Boris M Todurov
The thymus is the major site of T lymphocyte generation and so is critical for a functional adaptive immune system. Since, thymectomy is a component of neonatal surgery for congenital heart diseases, it provides great potential for collection and storage of thymic tissue for autologous transplantation. However, specific investigation into the optimum parameters for thymic tissue cryopreservation have not been conducted. In this research, we evaluated the effect of different cryoprotective media compositions, which included penetrating (Me2SO, glycerol) and non-penetrating (dextran-40, sucrose, hydroxyethyl starch) components, on the viability and functionality of frozen-thawed human thymic samples to select an optimal cryoprotective medium suitable for long-term storage of thymic tissue and a stromal-epithelial enriched population...
June 28, 2017: Cryobiology
https://www.readbyqxmd.com/read/28650975/defective-glucocorticoid-receptor-signaling-and-keratinocyte-autonomous-defects-contribute-to-skin-phenotype-of-mouse-embryos-lacking-the-hsp90-co-chaperone-p23
#7
Marta Madon-Simon, Iwona Grad, Pilar Bayo, Paloma Pérez, Didier Picard
p23 is a small acidic protein with intrinsic molecular chaperone activity. It is best known as a co-chaperone of the major cytosolic molecular chaperone Hsp90. p23 binds the N-terminus of Hsp90 and stabilizes the ATP-bound and N-terminally closed Hsp90 dimer. It is in this configuration that many Hsp90 clients are most stably bound. Considering the important role of p23 in the Hsp90 cycle, it came as a surprise that it is not absolutely essential for viability in the budding yeast or for mouse development. Mice without p23 develop quite normally until birth and then all die perinatally because of immature lungs...
2017: PloS One
https://www.readbyqxmd.com/read/28632489/the-effects-of-platelet-gel-on-cultured-human-retinal-pigment-epithelial-cells
#8
Sahar Balagholi, Shaban Alizadeh, Abouzar Bagheri, Yashar Amizadeh, Mozhgan Rezaei Kanavi
The positive role of platelet gel (PG) in tissue regeneration is well known, however, other characteristics of PG still remain to be determined. We investigated cellular and molecular changes in cultured human retinal pigment epithelial (hRPE) cells when treated with different concentrations of PG named PG1, PG2, and PG3. hRPE cells were isolated from donor eyes of two newborn children, within 24 hours after their death. The cells were treated with three concentrations of PG for 7 days: 3 × 104/ml (PG1), 6 × 104/ml (PG2), and 9 × 104/ml (PG3)...
June 20, 2017: Bosnian Journal of Basic Medical Sciences
https://www.readbyqxmd.com/read/28622787/monitoring-of-the-newborn-dog-and-prediction-of-neonatal-mortality
#9
Hanna Mila, Aurélien Grellet, Marine Delebarre, Claire Mariani, Alexandre Feugier, Sylvie Chastant-Maillard
Despite the high neonatal mortality rate in puppies, pertinent criteria for health evaluation of the newborns are not defined. This study was thus designed to measure and to characterize factors of variation of six health parameters in dog neonates, and to evaluate their value as predictors of neonatal mortality. A total of 347 purebred puppies under identical conditions of housing and management were examined within the first 8h after birth and then at Day 1. The first health evaluation included Apgar score, weight, blood glucose, lactate and β-hydroxybutyrate concentration, rectal temperature and urine specific gravity (SG)...
August 1, 2017: Preventive Veterinary Medicine
https://www.readbyqxmd.com/read/28612543/-the-improvement-in-the-primary-culture-of-alveolar-epithelial-type-of-rats
#10
Ling Jiang, Yuan-Dong Hu, Fei-Fei Xu, Ting-Hua Wang
OBJECTIVES: To improve the method of culturing and obtain purified alveolar epithelial type1 (AT1) cells of SD rats. METHODS: 1 d newborn SD rats were applied for cell culture and brains were decapitated for lung tissues obtaining after respiratory cessation. Collagenase1and DNase1 were used to digest and isolate cells. Then, cells were put into the plate coated with type1 rat tail collagen and different adherence was used to purify cells. Meanwhile, culture medium replacement was conducted after 48 h...
March 2017: Sichuan da Xue Xue Bao. Yi Xue Ban, Journal of Sichuan University. Medical Science Edition
https://www.readbyqxmd.com/read/28602654/the-human-fetus-preferentially-engages-with-face-like-visual-stimuli
#11
Vincent M Reid, Kirsty Dunn, Robert J Young, Johnson Amu, Tim Donovan, Nadja Reissland
In the third trimester of pregnancy, the human fetus has the capacity to process perceptual information [1-3]. With advances in 4D ultrasound technology, detailed assessment of fetal behavior [4] is now possible. Furthermore, modeling of intrauterine conditions has indicated a substantially greater luminance within the uterus than previously thought [5]. Consequently, light conveying perceptual content could be projected through the uterine wall and perceived by the fetus, dependent on how light interfaces with maternal tissue...
June 19, 2017: Current Biology: CB
https://www.readbyqxmd.com/read/28541264/basement-membrane-matrix-promotes-the-efficiency-of-hair-follicle-reconstruction-in-vivo-and-is-a-cell-delivery-vehicle-for-follicle-regeneration
#12
Shune Xiao, Yong Miao, Jin Wang, Bojie Lin, Zhiqi Hu
BACKGROUND: The efficiency of hair follicle (HF) reconstruction is decreased by extensive apoptotic remodeling that occurs soon after grafting. OBJECTIVE: To evaluate a basement membrane matrix (matrix) to improve the efficiency of HF reconstruction and serve as a cell delivery vehicle. MATERIALS AND METHODS: Newborn mouse skin cells were suspended in a matrix and transplanted in a chamber assay. The viability and proliferation of mouse dermal papilla cells seeded in the matrix were tested...
June 2017: Dermatologic Surgery: Official Publication for American Society for Dermatologic Surgery [et Al.]
https://www.readbyqxmd.com/read/28528122/probdnf-inhibits-proliferation-migration-and-differentiation-of-mouse-neural-stem-cells
#13
Jia-Yi Li, Jia Liu, Nimshitha Pavathuparambil Abdul Manaph, Larisa Bobrovskaya, Xin-Fu Zhou
ProBDNF, a precursor of brain-derived neurotrophic factor (BDNF), is an important regulator of neurodegeneration, hippocampal long-term depression, and synaptic plasticity. ProBDNF and its receptors pan-neurotrophin receptor p75 (p75NTR), vps10p domain-containing receptor Sortilin and tropomyosin receptor kinase B (TrkB) are expressed in neuronal and glial cells. The role of proBDNF in regulation of neurogenesis is not fully defined. This study aims to uncover the function of proBDNF in regulating the differentiation, migration and proliferation of mouse neural stem cells (NSCs) in vitro...
May 17, 2017: Brain Research
https://www.readbyqxmd.com/read/28407851/time-dependent-changes-and-prognostic-value-of-lactatemia-during-the-first-24%C3%A2-h-of-life-in-brachycephalic-newborn-dogs
#14
C Castagnetti, M Cunto, C Bini, J Mariella, S Capolongo, D Zambelli
Blood lactate concentration is known to be a good prognostic indicator associated with the severity of illness and the patient's outcome both in human and veterinary medicine. It also plays a significant role in the assessment of the newborn, being a good indicator of fetal hypoxia and the ideal predictor of morbidity at term in babies. In veterinary neonatal medicine, hyperlactatemia is considered a valid prognostic marker in critically ill foals; moreover, blood lactate measurement has been proposed for the evaluation of newborn viability and the assessment of fetal distress during delivery in dogs...
May 2017: Theriogenology
https://www.readbyqxmd.com/read/28249771/effects-of-the-copy-number-of-ribosomal-genes-genes-for-rrna-on-viability-of-subjects-with-chromosomal-abnormalities
#15
N A Lyapunova, L N Porokhovnik, N V Kosyakova, I A Mandron, T G Tsvetkova
The number of active ribosomal genes (AcRG) was evaluated in 172 carriers of chromosomal abnormalities (CA) such as Down's syndrome (DS), Robertsonian translocations (RT), Klinefelter's and Turner's syndromes, trisomy Х, disomy Y, and various structural CA. In controls (n=318), AcRG dosage varied from 119 to 190 copies with a mean of 151 copies per diploid genome. In CA carriers, except for DS newborns, AcRG dosage was not beyond these limits. As shown previously, only within these limits cellular homeostasis and organism's viability can be supported, while genomes beyond these limits are eliminated by embryonic loss...
May 5, 2017: Gene
https://www.readbyqxmd.com/read/28228611/anti-diabetic-action-of-all-trans-retinoic-acid-and-the-orphan-g-protein-coupled-receptor-gprc5c-in-pancreatic-%C3%AE-cells
#16
Stefan Amisten, Israa Mohammad Al-Amily, Arvind Soni, Ross Hawkes, Patricio Atanes, Shanta Jean Persaud, Patrik Rorsman, Albert Salehi
Pancreatic islets express high levels of the orphan G-protein coupled receptor C5C (GPRC5C), the function of which remains to be established. Here we have examined the role of GPRC5C in the regulation of insulin secretion and β-cell survival and proliferation using human and mouse pancreatic islets. The expression of GPRC5C was analysed by RNA-sequencing, qPCR, western blotting and confocal microscopy. Insulin secretion and cell viability were determined by RIA and MTS assays, respectively. GPRC5C mRNA expression and protein level were reduced in the islets from type-2 diabetic donors...
March 31, 2017: Endocrine Journal
https://www.readbyqxmd.com/read/28188727/apoptin-gene-delivery-by-the-functionalized-polyamidoamine-dendrimer-derivatives-induces-cell-death-of-u87-mg-glioblastoma-cells
#17
Yoonhee Bae, Hyang-Shuk Rhim, Seulgi Lee, Kyung Soo Ko, Jin Han, Joon Sig Choi
Malignant glioma is the most common and aggressive form of primary brain tumor in adults. In this study, we describe the efficacy of nonviral gene delivery carriers, histidine- and arginine- or histidine- and lysine-grafted polyamidoamine (PAMAM) dendrimers (PAMAM-H-R and PAMAM-H-K), in delivering a therapeutic and a tumor-selective killer gene, apoptin, using human glioma cells (U87-MG) and newborn human dermal fibroblast cells. We analyzed transfection efficiency using luciferase and a plasmid DNA encoding for enhanced green fluorescent protein and assessed cell viability in both cells...
February 7, 2017: Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/28162174/-viability-of-osteoblasts-under-cell-hypoxia-condition
#18
C Wu, W W Zheng, M Yang
Objective: To investigate the effects of hypoxia condition and hypoxia-reoxygenation condition on the cell viability, apoptosis rate and gene expression of osteoblasts cultured in vitro. Methods: The cranium osteoblasts from newborn Sprague Dawley rats within 48 hours were cultured and purified through tissue block method.The morphological changes of cells were evaluated by the Alizarin Red S staining and Alkaline phosphatase staining.The third-generation osteoblasts were cultured in normal condition for 36 hours (group A), in hypoxic condition for 24hours (group B), in hypoxic condition for 24hours thereafter reoxygenated for 12 hours (group D), in hypoxic condition for 36 hours (group C)...
January 17, 2017: Zhonghua Yi Xue za Zhi [Chinese medical journal]
https://www.readbyqxmd.com/read/28140628/online-gait-learning-for-modular-robots-with-arbitrary-shapes-and-sizes
#19
Berend Weel, M D'Angelo, Evert Haasdijk, A E Eiben
Evolutionary robotics using real hardware is currently restricted to evolving robot controllers, but the technology for evolvable morphologies is advancing quickly. Rapid prototyping (3D printing) and automated assembly are the main enablers of robotic systems where robot offspring can be produced based on a blueprint that specifies the morphologies and the controllers of the parents. This article addresses the problem of gait learning in newborn robots whose morphology is unknown in advance. We investigate a reinforcement learning method and conduct simulation experiments using robot morphologies with different size and complexity...
2017: Artificial Life
https://www.readbyqxmd.com/read/28102362/evaluation-of-region-selective-bilirubin-induced-brain-damage-as-a-basis-for-a-pharmacological-treatment
#20
Matteo Dal Ben, Cristina Bottin, Fabrizio Zanconati, Claudio Tiribelli, Silvia Gazzin
The neurologic manifestations of neonatal hyperbilirubinemia in the central nervous system (CNS) exhibit high variations in the severity and appearance of motor, auditory and cognitive symptoms, which is suggestive of a still unexplained selective topography of bilirubin-induced damage. By applying the organotypic brain culture (OBC: preserving in vitro the cellular complexity, connection and architecture of the in vivo brain) technique to study hyperbilirubinemia, we mapped the regional target of bilirubin-induced damage, demonstrated a multifactorial toxic action of bilirubin, and used this information to evaluate the efficacy of drugs applicable to newborns to protect the brain...
January 19, 2017: Scientific Reports
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