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Human serum albumin

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https://www.readbyqxmd.com/read/28932884/facile-and-easily-popularized-synthesis-of-l-cysteine-functionalized-magnetic-nanoparticles-based-on-one-step-functionalization-for-highly-efficient-enrichment-of-glycopeptides
#1
Xiaoyan Feng, Chunhui Deng, Mingxia Gao, Xiangmin Zhang
Protein glycosylation is one of the most important post-translational modifications. Also, efficient enrichment and separation of glycopeptides from complex samples are crucial for the thorough analysis of glycosylation. Developing novel hydrophilic materials with facile and easily popularized synthesis is an urgent need in large-scale glycoproteomics research. Herein, for the first time, a one-step functionalization strategy based on metal-organic coordination was proposed and Fe3O4 nanoparticles were directly functionalized with zwitterionic hydrophilic L-cysteine (L-Cys), greatly simplifying the synthetic procedure...
September 20, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28930631/direct-evidence-of-intrinsic-blue-fluorescence-from-oligomeric-interfaces-of-human-serum-albumin
#2
Arpan Bhattacharya, Soumitra Bhowmik, Amit K Singh, Prashant Kodgire, Apurba K Das, Tushar Kanti Mukherjee
The molecular origin behind the concentration-dependent intrinsic blue fluorescence of human serum albumin (HSA) is not known yet. This unusual blue fluorescence is believed to be a characteristic feature of amyloid-like fibrils of protein/peptide and originates due to the delocalization of peptide bond electrons through the extended hydrogen bond networks of cross--sheet structure. Herein, by combining the results of spectroscopy, size exclusion chromatography, native gel electrophoresis and confocal microscopy, we have shown that the intrinsic blue fluorescence of HSA exclusively originates from oligomeric interfaces devoid of any amyloid-like fibrillar structure...
September 20, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28930473/molecular-insights-into-human-serum-albumin-as-a-receptor-of-amyloid-%C3%AE-in-the-extracellular-region
#3
Tae Su Choi, Hyuck Jin Lee, Jong Yoon Han, Mi Hee Lim, Hugh I Kim
Regulation of amyloid-β (Aβ) aggregation by metal ions and proteins is essential for understanding the pathology of Alzheimer's disease (AD). Human serum albumin (HSA), a regulator of metal and protein transportation, can modulate metal-Aβ interactions and Aβ aggregation in human fluid; however, the molecular mechanisms for such activities remain unclear. Herein, we report the molecular-level complexation between Zn(II), Cu(II), Aβ, and HSA, which is able to alter the aggregation and cytotoxicity of Aβ peptides and induce their cellular transportation...
September 20, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/28930179/self-assembly-of-human-serum-albumin-a-simplex-phenomenon
#4
Garima Thakur, Kovur Prashanthi, Keren Jiang, Thomas Thundat
Spontaneous self-assemblies of biomolecules can generate geometrical patterns. Our findings provide an insight into the mechanism of self-assembled ring pattern generation by human serum albumin (HSA). The self-assembly is a process guided by kinetic and thermodynamic parameters. The generated protein ring patterns display a behavior which is geometrically related to a n-simplex model and is explained through thermodynamics and chemical kinetics.
September 20, 2017: Biomolecules
https://www.readbyqxmd.com/read/28929101/current-trends-in-volume-replacement-therapy-and-the-use-of-synthetic-colloids-in-small-animals-an-internet-based-survey-2016
#5
Ivayla D Yozova, Judith Howard, Nadja E Sigrist, Katja-Nicole Adamik
The use of synthetic colloids (SCs), particularly hydroxyethyl starch (HES), in people has changed in recent years following new evidence raising concerns about their efficacy and safety. Although fluid therapy guidelines for small animals are often extrapolated from human medicine, little information exists on current practice in veterinary medicine. The objective of the present study was to investigate current fluid selection, use of plasma volume expanders including SCs, and recent changes in their use in small animal practice...
2017: Frontiers in Veterinary Science
https://www.readbyqxmd.com/read/28928518/therapeutic-evaluation-of-grain-based-functional-food-formulation-in-a-geriatric-animal-model
#6
Deepa Teradal, Neena Joshi, Ravindranath H Aladakatti
This study investigates the effect of wholesome grain based functional food formulation, on clinical and biochemical parameters in 24-30 months old Wistar albino geriatric rats, corresponding to human age 60-75 years. Animals were randomly divided into five, groups. Experimental diets were compared to the basal rat diet (Group I). Four food, formulation were-wheat based (Group II), finger millet based (Group III), wheat based, diet + fenugreek seed powder (Group IV), finger millet based diet + fenugreek powder, (Group V)...
August 2017: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/28926772/ultrasound-assisted-interaction-between-chlorin-e6-and-human-serum-albumin-ph-dependence-singlet-oxygen-production-and-formulation-effect
#7
Mihaela N Mocanu, Fei Yan
The interaction between chlorin e6 (Ce6) and human serum albumin (HSA) in the presence and absence of ultrasound have been investigated by ultraviolet-visible absorption spectroscopy and fluorescence spectroscopy. Ce6 is found to bind strongly to HSA at or near physiological pH conditions, but the strength of the binding is significantly weakened at lower pHs. The intrinsic fluorescence of HSA is incrementally quenched with increasing concentration of Ce6, and the quenching is enhanced after exposure to high-frequency ultrasound...
September 13, 2017: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/28926756/warfarin-inhibits-allosterically-the-reductive-nitrosylation-of-ferric-human-serum-heme-albumin
#8
Paolo Ascenzi, Alessio Bocedi, Magda Gioia, Gabriella Fanali, Mauro Fasano, Massimo Coletta
Human serum heme-albumin (HSA-heme-Fe) displays heme-based ligand binding and (pseudo-)enzymatic properties. Here, the effect of the prototypical drug warfarin on kinetics and thermodynamics of NO binding to ferric and ferrous HSA-heme-Fe (HSA-heme-Fe(III) and HSA-heme-Fe(II), respectively) and on the NO-mediated reductive nitrosylation of the heme-Fe atom is reported; data were obtained between pH5.5 and 9.5 at 20.0°C. Since warfarin is a common drug, its effect on the reactivity of HSA-heme-Fe represents a relevant issue in the pharmacological therapy management...
September 5, 2017: Journal of Inorganic Biochemistry
https://www.readbyqxmd.com/read/28925865/structural-changes-of-human-serum-albumin-hsa-in-simulated-microgravity
#9
Azadeh Hekmat, Zahra Hajebrahimi, Amir Motamedzade
Nowadays, the biological effects of microgravity have been the subject of various experimental researches. The purpose of this study was to investigate the probable biological effects of microgravity on the human serum albumin (HSA) structure after 3 and 24 h exposure via various spectroscopic instruments. The UV-Visible, near-UV-CD and intrinsic fluorescence spectroscopy represented that microgravity can remarkably change the tertiary structure of HSA. Additionally, the ANS affinity for HSA incremented when the protein was exposed to simulate microgravity compared to unexposed HSA, which may possibly have appeared attributable to expansion of the structure of simulated HSA...
September 17, 2017: Protein and Peptide Letters
https://www.readbyqxmd.com/read/28923343/association-among-total-serum-isomers-of-perfluorinated-chemicals-glucose-homeostasis-lipid-profiles-serum-protein-and-metabolic-syndrome-in-adults-nhanes-2013-2014
#10
Hui-Shan Liu, Li-Li Wen, Pei-Lun Chu, Chien-Yu Lin
Perfluorinated chemicals (PFCs) have been used widely in consumer products manufacture. Recent in vitro as well as animal studies have found that there are different toxicity and pharmacokinetic profiles between isomers of perfluorooctanoic acid (PFOA) and/or perfluorooctane sulfonate (PFOS). However, the differential effects of linear or branched PFOA/PFOS isomers on human beings have never been reported. Herein, we examined 1871 adult subjects (age older than 18 years) from the National Health and Nutrition Examination Survey (NHANES) 2013-2014 to determine the association between the isomers of PFOA/PFOS and serum biochemistry profiles, including glucose, lipids, protein and components of metabolic syndrome (MS)...
September 15, 2017: Environmental Pollution
https://www.readbyqxmd.com/read/28922725/ameliorative-impact-of-morus-alba-leaves-aqueous-extract-against-embryonic-ophthalmic-tissue-malformation-in-streptozotocin-induced-diabetic-rats
#11
Adel M A Assiri, Mohamed E El-Beeh, Ali H Amin, Mohamed Fawzy Ramadan
BACKGROUND: Diabetes mellitus (DM) is becoming a serious threat to human health. Morus alba var. multicaulis (Perr.) Loudon (Moraceae) showed a bright future in DM therapy. OBJECTIVE: The study evaluates the antioxidant activity of Morus alba leaves aqueous extract (MLAE) and antidiabetic properties of MLAE in streptozotocin-induced diabetic rats focusing on the ameliorative effects against embryogenesis defects. MATERIALS AND METHODS: MLAE was assayed for bioactive compounds, and antiradical potential...
September 13, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28921971/paper-sensor-coated-with-a-poly-carboxybetaine-multiple-dopa-conjugate-via-dip-coating-for-biosensing-in-complex-media
#12
Fang Sun, Kan Wu, Hsiang-Chieh Hung, Peng Zhang, Xinran Che, Joshua Kenyon Smith, Xiaojie Lin, Bowen Li, Priyesh Jain, Qiuming Yu, Shaoyi Jiang
Cellulose paper is an ideal diagnostic platform for low-cost, easily disposable and lightweight implementation, but requires surface modification to achieve detection with high sensitivity and specificity in complex media. In this work, a polymer-catechol conjugate containing a super-hydrophilic nonfouling poly(carboxylbetaine) (pCB) and four surface-binding L-3,4-dihydroxyphenylalanine (DOPA) groups, pCB-(DOPA)4, were applied onto a paper-based sensor surface via a simple "graft-to" immersion process to render the surface with both nonfouling and protein functionalizable properties...
September 18, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28920943/expression-function-and-regulation-of-the-embryonic-transcription-factor-tbx1-in-parathyroid-tumors
#13
Chiara Verdelli, Laura Avagliano, Vito Guarnieri, Filomena Cetani, Stefano Ferrero, Leonardo Vicentini, Edoardo Beretta, Alfredo Scillitani, Pasquale Creo, Gaetano Pietro Bulfamante, Valentina Vaira, Sabrina Corbetta
Transcription factors active in embryonic parathyroid cells can be maintained in adult parathyroids and be involved in tumorigenesis. TBX1, the candidate gene of 22q11.2-DiGeorge syndrome, which includes congenital hypoparathyroidism, is involved in parathyroid embryogenesis. The study aimed to investigate expression, function, and regulation of the parathyroid embryonic transcription factor TBX1 in human parathyroid adult normal and tumor tissues. TBX1 transcripts were detected in normal parathyroids and were deregulated in parathyroid tumors...
September 18, 2017: Laboratory Investigation; a Journal of Technical Methods and Pathology
https://www.readbyqxmd.com/read/28920905/electrochemical-impedance-spectroscopy-on-interdigitated-gold-microelectrodes-for-glycosylated-human-serum-albumin-characterization
#14
Nadra Bohli, Hanen Chammem, Olivier Meilhac, Laurence Mora, Adnane Abdelghani
Glycosylated albumin is considered as a potentially accurate indicator of shorter-term average glucose concentration compared to the current standard HbA1c and as such, it's attracting the interest of the scientific community as a possible diagnosis marker for diabetic patients. The purpose of this paper is to achieve a better understanding of the glycation effect of albumin on its electrochemical properties. That is done through the use of Interdigitated gold microelectrodes (IDGE) as support in a label free impedimetric immunosensor for detection of human serum albumin detection in glycated (GA) and non-glycated (HSA) form...
September 14, 2017: IEEE Transactions on Nanobioscience
https://www.readbyqxmd.com/read/28920440/unrestricted-mass-spectrometric-data-analysis-for-identification-localization-and-quantification-of-oxidative-protein-modifications
#15
Martin Rykær, Birte Svensson, Michael J Davies, Per Hägglund
Oxidation generates multiple diverse post-translational modifications resulting in changes in protein structure and function, associated with a wide range of diseases. Of these modifications, carbonylations have often been used as hallmarks of oxidative damage. However, accumulating evidence supports the hypothesis that other oxidation products may be quantitatively more important under physiological conditions. To address this issue we have developed a holistic mass spectrometry-based approach for simultaneous identification, localization and quantification of a broad range of oxidative modifications based on so-called 'dependent peptides'...
September 18, 2017: Journal of Proteome Research
https://www.readbyqxmd.com/read/28918601/effects-of-phoxim-induced-hepatotoxicity-on-sd-rats-and-the-protection-of-vitamin-e
#16
Jing Zhang, Wentao Song, Yuecheng Sun, Anshan Shan
Currently, public pay more attention to the adverse effect of organophosphate pesticides on human and animal health and on the environment in developing nations. Vitamin E may protect the hepatocyte and increase the function of liver. The study was to investigate the effects of phoxim-induced hepatotoxicity on Sprague Dawley (SD) rats and the protection of vitamin E. SD rats received by gavage 180 mg kg(-1) (per body weight) of phoxim, 200 mg kg(-1) (per body weight) of vitamin E, and phoxim + vitamin E...
September 16, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28917756/facile-synthesis-of-copper-ii-decorated-functional-mesoporous-material-for-specific-adsorption-of-histidine-rich-proteins
#17
Jiaojiao Wang, Rui Zhang, Xiaxia Yang, Xiaoyan Liu, Haixia Zhang
The Cu(2+)-decorated functional mesoporous material was fabricated by thermally initiated free-radical polymerization of octavinyl polyhedral oligomeric silsesquioxane. It was used as adsorbent for highly specific separation of histidine (His)-rich proteins in blood and cell lysate based on immobilized metal affinity chromatography. The functional mesoporous material (named as PPOSS-IDA-Cu(2+)) was characterized in detail and its selectivity and binding capacity were evaluated using a His-rich protein (bovine hemoglobin, BHb) and other proteins (bovine serum albumin, myoglobin, lysozyme and horseradish peroxidase) containing fewer surface-exposed His residues as model proteins...
January 1, 2018: Talanta
https://www.readbyqxmd.com/read/28917201/in-silico-investigation-of-propofol-binding-sites-in-human-serum-albumin-using-explicit-and-implicit-solvation-models
#18
Sergey Shityakov, Norbert Roewer, Carola Förster, Jens-Albert Broscheit
All-atom molecular dynamics (MD) simulations are presented on general anesthetic propofol bound to human serum albumin (HSA) due to the drug pharmacokinetics and pharmacodynamics in the circulatory system. We implemented the explicit and implicit solvation models to compare the binding affinity of propofol at the different binding sites (PR1 and PR2) in the HSA protein. Only the implicit solvation models provided the evidence in accordance with the experimental data indicating that the HSA-ligand interactions are dominanted by hydrophobic forces due to the higher drug affinity at the PR1 position with a ΔGMM-PB/SA value of -23...
July 4, 2017: Computational Biology and Chemistry
https://www.readbyqxmd.com/read/28915532/formation-mechanism-of-human-serum-albumin-monolayers-on-positively-charged-polymer-microparticles
#19
Małgorzata Nattich-Rak, Marta Sadowska, Zbigniew Adamczyk, Michał Cieśla, Małgorzata Kąkol
Human serum albumin (HSA) adsorption on positively and negatively charged polystyrene microparticles was studied at various pHs and NaCl concentrations. Thorough electrophoretic mobility measurements were carried out that enabled to monitor in situ the progress of protein adsorption. The maximum coverage of irreversibly adsorbed HSA on microparticles was determined by different concentration depletion methods, one of them involving AFM imaging of residual molecules. An anomalous adsorption of HSA on the positive microparticles was observed at pH 3...
August 31, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28915468/spectral-and-computational-features-of-the-binding-between-riparins-and-human-serum-albumin
#20
Cintia Ramos Camargo, Ícaro Putinhon Caruso, Stanley Juan Chavez Gutierrez, Marcelo Andres Fossey, José Maria Barbosa Filho, Marinônio Lopes Cornélio
The green Brazilian bay leaf, a spice much prized in local cuisine (Aniba riparia, Lauraceae), contains chemical compounds presenting benzoyl-derivatives named riparins, which have anti-inflammatory, antimicrobial and anxiolytic properties. However, it is unclear what kind of interaction riparins perform with any molecular target. As a profitable target, human serum albumin (HSA) is one of the principal extracellular proteins, with an exceptional capacity to interact with several molecules, and it also plays a crucial role in the transport, distribution, and metabolism of a wide variety of endogenous and exogenous ligands...
September 8, 2017: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
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