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Human serum albumin

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https://www.readbyqxmd.com/read/29332139/effect-of-host-human-products-on-natural-transformation-in-acinetobacter-baumannii
#1
Brettni Quinn, German M Traglia, Meaghan Nguyen, Jasmine Martinez, Christine Liu, Jennifer S Fernandez, Maria Soledad Ramirez
Our previous data show that serum albumin can trigger natural transformation in Acinetobacter baumannii. However, extracellular matrix/basal membrane components, norepinephrine, and mucin did not have a significant effect on this process. Therefore, the effect of human products appears to be albumin specific, as both BSA and HSA have been shown to increase of natural transformation.
January 13, 2018: Current Microbiology
https://www.readbyqxmd.com/read/29332090/impaired-value-of-99m-tc-gsa-scintigraphy-as-an-independent-risk-factor-for-posthepatectomy-liver-failure-in-patients-with-hepatocellular-carcinoma
#2
Ikuo Nakamura, Yuji Iimuro, Seikan Hai, Yuichi Kondo, Etsuro Hatano, Jiro Fujimoto
BACKGROUND: Posthepatectomy liver failure (PHLF) was recently defined with the corresponding recommendations as follows: grade A, no change in clinical management; grade B, clinical management with noninvasive treatment; and grade C, clinical management with invasive treatment. In this study, we identified the risk factors for grade B and C PHLF in patients with hepatocellular carcinoma (HCC). METHODS: Of 339 HCC patients who underwent curative hepatic resection, 218 were included for analysis...
January 12, 2018: European Surgical Research. Europäische Chirurgische Forschung. Recherches Chirurgicales Européennes
https://www.readbyqxmd.com/read/29330129/estradiol-up-regulates-l-type-ca2-channels-via-membrane-bound-estrogen-receptor-phosphoinositide-3kinase-akt-camp-response-element-binding-protein-signaling-pathway
#3
Xiaoyan Yang, Xiaofang Mao, Gao Xu, Shasha Xing, Ansuman Chattopadhyay, Si Jin, Guy Salama
BACKGROUND: In long QT type-2 (LQT2), women are more prone to lethal arrhythmias called Torsade de Pointes (TdP) than men. We previously reported that 17-β-estradiol (E2) upregulates L-type Ca2+-channels and current (ICa,L) (∼30%) in rabbit ventricular myocytes by a classical genomic-mechanism mediated by estrogen-receptor-α (ER)α. In LQT2 ( IKr-blockade or bradycardia), the higher Ca2+ influx via ICa,L, causes Ca2+-overload, spontaneous sarcoplasmic reticulum Ca2+-release, and re-activation of ICa,L that trigger early afterdepolarizations (EADs) and TdP...
January 9, 2018: Heart Rhythm: the Official Journal of the Heart Rhythm Society
https://www.readbyqxmd.com/read/29329699/soluble-cd40-ligand-disrupts-the-blood-brain-barrier-and-exacerbates-inflammation-in-experimental-autoimmune-encephalomyelitis
#4
Hiroki Masuda, Masahiro Mori, Kenta Umehara, Tomomi Furihata, Tomohiko Uchida, Akiyuki Uzawa, Satoshi Kuwabara
Serum soluble CD40 ligand (sCD40L) has been reported to positively correlate with the albumin quotient, a marker of blood-brain barrier (BBB) breakdown, in patients with multiple sclerosis (MS). To clarify the mechanisms of sCD40L in MS pathophysiology, sCD40L was administered to experimental autoimmune encephalomyelitis (EAE) mice and a human brain microvascular endothelial cell (HBMEC)-based BBB model. The high-dose sCD40L group showed a worse EAE score than the low-dose and control groups. BBB permeability was increased by administering sCD40L in a HBMEC-based BBB model...
January 3, 2018: Journal of Neuroimmunology
https://www.readbyqxmd.com/read/29328686/three-dimensional-tracking-of-interfacial-hopping-diffusion
#5
Dapeng Wang, Haichao Wu, Daniel K Schwartz
Theoretical predictions have suggested that molecular motion at interfaces-which influences processes including heterogeneous catalysis, (bio)chemical sensing, lubrication and adhesion, and nanomaterial self-assembly-may be dominated by hypothetical "hops" through the adjacent liquid phase, where a diffusing molecule readsorbs after a given hop according to a probabilistic "sticking coefficient." Here, we use three-dimensional (3D) single-molecule tracking to explicitly visualize this process for human serum albumin at solid-liquid interfaces that exert varying electrostatic interactions on the biomacromolecule...
December 29, 2017: Physical Review Letters
https://www.readbyqxmd.com/read/29328653/extending-half-life-of-h-ferritin-nanoparticle-by-fusing-albumin-binding-domain-for-doxorubicin-encapsulation
#6
Chunyue Wang, Chun Zhang, Zenglan Li, Shuang Yin, Qi Wang, Fangxia Guo, Yao Zhang, Rong Yu, Yongdong Liu, Zhiguo Su
Nanoparticles based on the heavy chain of the human ferritin (HFn) are arousing growing interest in the field of drug-delivery due to their exceptional characteristics. However, the unsatisfied plasma half-life of HFn substantially limits its application as a delivery platform for anti-tumor agents. Herein, we fused an albumin binding domain (ABD) variant which basically derives from the streptococcal protein G and possesses a long-acting characteristic in serum albumin to the N-terminus of the HFn for the aim of half-life extension...
January 12, 2018: Biomacromolecules
https://www.readbyqxmd.com/read/29327435/impact-of-dietary-induced-precocious-gut-maturation-on-cecal-microbiota-and-its-relation-to-the-blood-brain-barrier-during-the-postnatal-period-in-rats
#7
N Marungruang, E Arévalo Sureda, A Lefrançoise, B Weström, M Nyman, O Prykhodko, F Fåk Hållenius
BACKGROUND: Precocious maturation of the gastrointestinal barrier (GIB) in newborn mammals can be induced by dietary provocation, but how this affects the gut microbiota and the gut-brain axis remains unknown. The objective of this study was to investigate effects of induced GIB maturation on gut microbiota composition and blood-brain barrier (BBB) permeability. METHODS: Suckling rats were studied at 72 h after gavage with phytohemagglutinin (PHA) or microbial protease (PT) to induce maturation of GIB...
January 12, 2018: Neurogastroenterology and Motility: the Official Journal of the European Gastrointestinal Motility Society
https://www.readbyqxmd.com/read/29327112/simultaneous-pre-concentration-and-separation-on-simple-paper-based-analytical-device-for-protein-analysis
#8
Ji-Cheng Niu, Ting Zhou, Li-Li Niu, Zhen-Sheng Xie, Fang Fang, Fu-Quan Yang, Zhi-Yong Wu
In this work, fast isoelectric focusing (IEF) was successfully implemented on an open paper fluidic channel for simultaneous concentration and separation of proteins from complex matrix. With this simple device, IEF can be finished in 10 min with a resolution of 0.03 pH units and concentration factor of 10, as estimated by color model proteins by smartphone-based colorimetric detection. Fast detection of albumin from human serum and glycated hemoglobin (HBA1c) from blood cell was demonstrated. In addition, off-line identification of the model proteins from the IEF fractions with matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS) was also shown...
January 11, 2018: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/29325755/amphotericin-b-albumin-conjugates-synthesis-toxicity-and-anti-fungal-activity
#9
Sneha Gurudevan, Arul Prakash Francis, A Jayakrishnan
Amphotericin B (AmB), a hydrophobic drug with negligible aqueous solubility was conjugated to bovine serum albumin (BSA) via amide bond coupling to give 6 to 8 wt% drug payload. The resulting conjugate was characterized using SDS-PAGE and UV-visible, FTIR and CD spectroscopy. The conjugate was water-soluble to the extent of 150 mg/ml, was non-toxic to HEK 293 T cells at a concentration of 500 μg/ml (equivalent to ~30 μg AmB) and showed hemolysis of <5% at 200 μg/ml (equivalent to ~12 μg AmB) against human erythrocytes in vitro...
January 8, 2018: European Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/29324720/biointeractions-of-herbicide-atrazine-with-human-serum-albumin-uv-vis-fluorescence-and-circular-dichroism-approaches
#10
Meiqing Zhu, Lijun Wang, Yu Wang, Jie Zhou, Jie Ding, Wei Li, Yue Xin, Shisuo Fan, Zhen Wang, Yi Wang
The herbicide atrazine is widely used across the globe, which is a great concern. To investigate its potential toxicity in the human body, human serum albumin (HSA) was selected as a model protein. The interaction between atrazine and HSA was investigated using steady-state fluorescence spectroscopy, synchronous fluorescence spectroscopy, UV-Vis spectroscopy, three-dimensional (3D) fluorescence spectroscopy and circular dichroism (CD) spectroscopy. The intrinsic fluorescence of HSA was quenched by the atrazine through a static quenching mechanism...
January 11, 2018: International Journal of Environmental Research and Public Health
https://www.readbyqxmd.com/read/29322412/in-vivo-models-for-inflammatory-arthritis
#11
Gareth W Jones, David G Hill, Katie Sime, Anwen S Williams
In vivo mouse models of inflammatory arthritis are extensively used to investigate pathogenic mechanisms governing inflammation-driven joint damage. Two commonly utilized models include collagen-induced arthritis (CIA) and methylated bovine serum albumin (mBSA) antigen-induced arthritis (AIA). These offer unique advantages for modeling different aspects of human disease. CIA involves breach of immunological tolerance resulting in systemic autoantibody-driven arthritis, while AIA results in local resolving inflammatory flares and articular T cell-mediated damage...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29322229/bioanalytical-verification-of-v-type-nerve-agent-exposure-simultaneous-detection-of-phosphonylated-tyrosines-and-cysteine-containing-disulfide-adducts-derived-from-human-albumin
#12
Andreas Kranawetvogl, Jim Küppers, Markus Siegert, Michael Gütschow, Franz Worek, Horst Thiermann, Paul W Elsinghorst, Harald John
Nerve agents still represent a serious threat to civilian and military personnel as demonstrated by the violent conflict in the Middle East. For verification of poisoning, covalent adducts with endogenous proteins (e.g., human serum albumin, HSA) are valuable long-term biomarkers. Accordingly, we developed a microbore liquid chromatography-electrospray ionization mass spectrometry/high-resolution mass spectrometry (μLC-ESI MS/HR MS) method for simultaneous detection of HSA-adducts with the V-type nerve agents VX, Chinese VX (CVX), and Russian VX (RVX)...
January 10, 2018: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/29317059/pathophysiological-significance-of-protein-hydrophobic-interactions-an-emerging-hypothesis
#13
Marek Kieliszek, Boguslaw Lipinski
Fibrinogen is a unique protein that is converted into an insoluble fibrin in a single enzymatic event, which is a characteristic feature of fibrinogen due to its susceptibility to fibrinolytic degradation and dissolution. Although thrombosis is a result of activated blood coagulation, no explanation is being offered for the persistent presence of fibrin deposits in the affected organs. A classic example is stroke, in which the thrombolytic therapy is effective only during the first 3-4 h after the onset of thrombosis...
January 2018: Medical Hypotheses
https://www.readbyqxmd.com/read/29316533/albumin-adsorption-at-solid-substrates-a-quest-for-a-unified-approach
#14
Zbigniew Adamczyk, Małgorzata Nattich-Rak, Maria Dąbkowska, Marta Kujda-Kruk
Adsorption of human serum albumin (HSA), recombinant HSA (rHSA) and the albumin dimer (dHSA) at solid/electrolyte interfaces is reviewed with the emphasis put on quantitative analysis of this process. Initially, various physicochemical data characterizing bulk properties of albumin molecules are discussed such as electrophoretic mobility, electrokinetic charge, zeta potential and diffusion coefficient. Adsorption kinetics of HSA, rHSA and dHSA at mica derived from AFM, streaming potential and XPS measurements is analyzed...
December 19, 2017: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/29316482/selective-binding-of-pyrene-in-subdomain-ib-of-human-serum-albumin-combining-energy-transfer-spectroscopy-and-molecular-modelling-to-understand-protein-binding-flexibility
#15
Irene Ling, Mohamed Taha, Nada A Al-Sharji, Osama K Abou-Zied
The ability of human serum albumin (HSA) to bind medium-sized hydrophobic molecules is important for the distribution, metabolism, and efficacy of many drugs. Herein, the interaction between pyrene, a hydrophobic fluorescent probe, and HSA was thoroughly investigated using steady-state and time-resolved fluorescence techniques, ligand docking, and molecular dynamics (MD) simulations. A slight quenching of the fluorescence signal from Trp214 (the sole tryptophan residue in the protein) in the presence of pyrene was used to determine the ligand binding site in the protein, using Förster's resonance energy transfer (FRET) theory...
January 4, 2018: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/29316385/analysis-of-recombinant-human-serum-albumin-extraction-degradation-in-transgenic-rice-extracts
#16
Kseniya A Sheshukova, Lisa R Wilken
Transgenic plant systems have successfully been used to express recombinant proteins, including rice seed-expressed recombinant human serum albumin (rHSA), without the risk of contamination of human pathogens. Developing an efficient extraction process is critical as the step determines recombinant protein concentration and purity, quantity of impurities, and process volume. This paper evaluates the effect of pH and time on the extraction and stability of rHSA. The amount of rHSA in clarified extract after 60 min of solubilization increased with pH from 0...
January 8, 2018: Biotechnology Progress
https://www.readbyqxmd.com/read/29315710/an-improved-design-to-capture-magnetic-microparticles-for-capillary-electrophoresis-based-immobilized-microenzyme-reactors
#17
Pranov Ramana, Jan Schejbal, Kristof Houthoofd, Johan Martens, Erwin Adams, Patrick Augustijns, Zdenĕk Glatz, Ann Van Schepdael
In this paper, we demonstrate the effectiveness of a new 3D printed magnet holder that enables capture of magnetic microparticles in commercially available capillary electrophoresis equipment with a liquid or air based coolant system. The design as well as the method to capture magnetic microparticles inside the capillary are discussed. This setup was tested at temperature and pH values suitable for performing enzymatic reactions. To demonstrate its applicability in CE- immobilized microenzyme reactors (IMER) development, human flavin-containing monooxygenase 3 and bovine serum albumin were immobilized on amino functionalized magnetic microparticles using glutaraldehyde...
January 5, 2018: Electrophoresis
https://www.readbyqxmd.com/read/29314828/prussian-blue-nanoparticles-as-a-catalytic-label-in-a-sandwich-nanozyme-linked-immunosorbent-assay
#18
Zdeněk Farka, Veronika Čunderlová, Veronika Horáčková, Matěj Pastucha, Zuzana Mikušová, Antonín Hlaváček, Petr Skládal
Enzyme immunoassays are widely used for detection of analytes within various samples. However, enzymes as labels suffer several disadvantages such as high production costs and limited stability. Catalytic nanoparticles (nanozymes) can be used as an alternative label in immunoassays overcoming the inherent disadvantages of enzymes. Prussian blue nanoparticles (PBNPs) are nanozymes composed of the Fe4[Fe(CN)6]3 based coordination polymer. They reveal peroxidase-like activity and are capable of catalyzing the oxidation of colorless 3,3',5,5'-tetramethylbenzidine in the presence of H2O2 to form intensively blue product...
January 9, 2018: Analytical Chemistry
https://www.readbyqxmd.com/read/29310697/epidemiology-genetic-variants-and-clinical-course-of-natural-infections-with-anaplasma-phagocytophilum-in-a-dairy-cattle-herd
#19
Cornelia Silaghi, Marion Nieder, Carola Sauter-Louis, Gabriela Knubben-Schweizer, Kurt Pfister, Martin Pfeffer
BACKGROUND: Anaplasma phagocytophilum is an obligate intracellular, tick-transmitted bacterium that causes granulocytic anaplasmosis in humans and several mammalian species including domestic ruminants where it is called tick-borne fever (TBF). Different genetic variants exist but their impact with regard to putative differences in host associations and pathogenicity are not yet completely understood. METHODS: Natural infections with A. phagocytophilum in a dairy cattle herd in Germany were investigated over one pasture season by using serology, haematology, blood chemistry and polymerase chain reaction (PCR)...
January 8, 2018: Parasites & Vectors
https://www.readbyqxmd.com/read/29310242/competitive-horseradish-peroxidase-linked-aptamer-assay-for-sensitive-detection-of-aflatoxin-b1
#20
Linlin Sun, Qiang Zhao
Aflatoxin B1 (AFB1) is one of highly toxic mycotoxins and a known human carcinogen. The frequent contamination of AFB1 in food products and large health risk of AFB1 have raised global concerns. Sensitive detection of AFB1 is of vital importance and highly demanded. Herein, we reported a competitive horseradish peroxidase (HRP)-linked aptamer assay for AFB1, combining the advantages of aptamer for affinity binding and enzyme label for signal amplification. In this assay, free AFB1 in solution competed with a covalent conjugate of bovine serum albumin-AFB1 (BSA-AFB1) coated on the wells of microplate in binding to the HRP-labeled aptamer probe...
March 1, 2018: Talanta
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