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Human serum albumin

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https://www.readbyqxmd.com/read/27912144/elucidation-of-%C3%AE-s-dynamics-of-domain-iii-of-human-serum-albumin-during-the-chemical-and-thermal-unfolding-a-fluorescence-correlation-spectroscopic-investigation
#1
Bhaswati Sengupta, Nilimesh Das, Pratik Sen
The local structural dynamics and denaturation profile of domain-III of HSA against guanidine hydrochloride (GnHCl) and temperature has been studied using a coumarin based solvatochromic fluorescent probe p-nitrophenyl coumarin ester (NPCE), covalently tagged to Tyr-411 residue. By the steady state, time-resolved and single molecular level fluorescence studies it has been established that the domain-III of HSA is very sensitive to GnHCl but somewhat resistant to temperature and the domain specific unfolding proceeds in an altered way as compared to the overall unfolding of HSA...
November 18, 2016: Biophysical Chemistry
https://www.readbyqxmd.com/read/27908378/erratum-to-cigarette-smoke-induces-alterations-in-the-drug-binding-properties-of-human-serum-albumin-blood-cells-mol-dis-52-4-2014-apr-166-174
#2
M Clerici, G Colombo, F Secundo, N Gagliano, R Colombo, N Portinaro, D Giustarini, A Milzani, R Rossi, I Dalle-Donne
No abstract text is available yet for this article.
November 2016: Blood Cells, Molecules & Diseases
https://www.readbyqxmd.com/read/27907865/biophysical-characterization-and-antineoplastic-activity-of-new-bis-thiosemicarbazonato-cu-ii-complexes
#3
Elisa Palma, Filipa Mendes, Goreti Ribeiro Morais, Inês Rodrigues, Isabel Cordeiro Santos, Maria Paula C Campello, Paula Raposinho, Isabel Correia, Sofia Gama, Dulce Belo, Vítor Alves, Antero J Abrunhosa, Isabel Santos, António Paulo
Aiming to explore alternative mechanisms of cellular uptake and cytotoxicity, we have studied a new family of copper(II) complexes (CuL(1)-CuL(4)) with bis(thiosemicarbazone) (BTSC) ligands containing pendant protonable cyclic amines (morpholine and piperidine). Herein, we report on the synthesis and characterization of these new complexes, as well as on their biological performance (cytotoxic activity, cellular uptake, protein and DNA binding), in comparison with the parental Cu(II)ATSM (ATSM=diacetyl-bis(N4-methylthiosemicarbazonate) complex without pendant cyclic amines...
November 23, 2016: Journal of Inorganic Biochemistry
https://www.readbyqxmd.com/read/27904073/a-case-of-familial-dysalbuminemic-hyperthyroxinemia-fdh-in-japan-fdh-as-a-possible-differential-diagnosis-of-syndrome-of-inappropriate-secretion-of-thyroid-stimulating-hormone-sitsh
#4
Sakiko Kobayashi, Jaeduk Yoshimura Noh, Taeko Shimizu, Tomoaki Sato, Isao Kurihara, Kiminori Sugino, Hiroshi Itoh, Koichi Ito
Familial dysalbuminemic hyperthyroxinemia (FDH) is an autosomal dominant condition and is the most commonly inherited euthyroid hyperthyroxinemia in Caucasians. However, it is extremely rare in Asian populations. A 30-year-old Japanese woman, who was incidentally found to have apparent thyroid dysfunction, was admitted to our hospital in 2004. She had extremely elevated serum free thyroxine (FT4), moderately elevated free triiodothyronine (FT3), and normal thyroid-stimulating hormone (TSH). Clinical thyroid examination revealed no abnormalities other than small goiter...
November 30, 2016: Endocrine Journal
https://www.readbyqxmd.com/read/27903492/angiotensinogen-import-in-isolated-proximal-tubules-evidence-for-mitochondrial-trafficking-and-uptake
#5
Bryan A Wilson, Nildris Cruz-Diaz, Yixin Su, James C Rose, TanYa M Gwathmey, Mark C Chappell
The renal proximal tubules are a key functional component of the kidney and express the angiotensin precursor angiotensinogen; however, it is unclear the extent that tubular angiotensinogen reflects local synthesis or internalization. Therefore, the current study established the extent that angiotensinogen is internalized by proximal tubules and the intracellular distribution. Proximal tubules were isolated from the kidney cortex of male sheep by enzymatic digestion and a discontinuous Percoll gradient. Tubules were incubated with radiolabeled 125I-angiotensinogen for 2 hrs at 37°C in serum/phenol-free DMEM/F12 media...
November 30, 2016: American Journal of Physiology. Renal Physiology
https://www.readbyqxmd.com/read/27902355/the-use-of-human-umbilical-cord-blood-serum-is-beneficial-for-the-continuous-production-of-hepatitis-c-virus
#6
Xiao-Yang Jiao, Rineke H G Steenbergen, D Lorne Tyrrell
In this study we investigated if human umbilical cord blood serum (CBS) is a suitable replacement for fetal bovine serum (FBS) in cultures of human hepatoma cell line Huh7.5, particularly regarding its capacity to maintain high growth rates, differentiation status and its ability to support robust HCV infection. Generally, CBS cultured Huh7.5 cells remained comparable to FBS cultured cells, and proliferated equally well. Albumin secretion, a hepatocyte differentiation marker, had increased 8x in CBS, however, most other hepatocyte markers we tested had not changed...
October 24, 2016: Journal of General Virology
https://www.readbyqxmd.com/read/27901544/quantitative-urinalysis-using-aggregation-induced-emission-bioprobes-for-monitoring-chronic-kidney-disease
#7
Tong Chen, Ni Xie, Lucia Viglianti, Yabin Zhou, Hui Tan, Ben Zhong Tang, Youhong Tang
Early detection and appropriate management of chronic kidney disease can reduce the progression of kidney failure and cardiovascular disease. The urine albumin to creatinine ratio (UACR) test is a standard urine test for identifying individuals at high risk of developing progressive kidney disease. In this study, IDATPE, a novel fluorescent probe with aggregation-induced emission (AIE) features, is successfully developed for creatinine detection and quantitation. An excellent correlation between fluorescent light intensity and creatinine concentration is achieved...
November 30, 2016: Faraday Discussions
https://www.readbyqxmd.com/read/27900432/novel-boronate-material-affords-efficient-enrichment-of-glycopeptides-by-synergized-hydrophilic-and-affinity-interactions
#8
Jianying Chen, Xiaohu Li, Mengyu Feng, Kun Luo, Juan Yang, Bo Zhang
Development of novel materials for enrichment of glycopeptides is the key to a comprehensive analysis of the glycoproteome, which is closely related to several major diseases and biomarker findings. We synthesized phenylboronic acid (PBA) bound to SiO2 microspheres by a thiol-ene click chemistry method (this material was denoted as click PBA) and used it to separate cis-diol-containing molecules and enrich glycopeptides in hydrophilic interaction chromatography mode. Successful preparation of click PBA was confirmed by elemental analysis, X-ray photoelectron spectroscopy, N2 adsorption-desorption isotherms, and high-resolution scanning electron microscopy...
November 29, 2016: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/27900373/10-nm-deep-sub-nanoliter-fluidic-nanochannels-on-germanium-for-attenuated-total-reflection-infrared-atr-ir-spectroscopy
#9
K K Sriram, Simantini Nayak, Stefanie Pengel, Chia-Fu Chou, Andreas Erbe
The fabrication of sub-nanoliter fluidic channels is demonstrated, with merely 10 nm depth on germanium, using conventional semiconductor device fabrication methods and a polymer assisted room-temperature sealing method. As a first application, an ultralow volume (650 pL) was studied by ATR-IR spectroscopy. A detection limit of ∼7.9 × 10(10) molecules of human serum albumin (HSA) (∼0.2 mM) in D2O was achieved with highly specific ATR-IR spectroscopy.
November 30, 2016: Analyst
https://www.readbyqxmd.com/read/27899066/predictive-factors-for-unfavorable-outcomes-of-tuberculous-pericarditis-in-human-immunodeficiency-virus-uninfected-patients-in-an-intermediate-tuberculosis-burden-country
#10
In Young Jung, Young Goo Song, Jun Yong Choi, Moo Hyun Kim, Woo Yong Jeong, Dong Hyun Oh, Yong Chan Kim, Je Eun Song, Eun Jin Kim, Ji Un Lee, Su Jin Jeong, Nam Su Ku, June Myung Kim
BACKGROUND: In areas where Mycobacterium tuberculosis is endemic, tuberculosis is known to be the most common cause of pericarditis. However, the difficulty in diagnosis may lead to late complications such as constrictive pericarditis and increased mortality. Therefore, identification of patients at a high risk for poor prognosis, and prompt initiation of treatment are important in the outcome of TB pericarditis. The aim of this study is to identify the predictive factors for unfavorable outcomes of TB pericarditis in HIV-uninfected persons in an intermediate tuberculosis burden country...
November 29, 2016: BMC Infectious Diseases
https://www.readbyqxmd.com/read/27897092/an-investigation-on-intermolecular-interaction-between-bis-indolyl-methane-and-hsa-and-bsa-using-multi-technique-methods
#11
Hamid Dezhampanah, Roghaye Firouzi
Bis(indolyl)methane (BIM) as one of the main active components of anticancer and antibacterial drugs is applied in medicinal and extensive area of chemistry. In this research interaction of human and bovine serum albumins, as drug carriers with BIM was investigated using spectroscopy methods and molecular modeling study. The fluorescence quenching measurements at the rage of 293 to 310 K revealed that the quenching mechanisms for human and bovine serum albumins are static and dynamic processes, respectively...
November 29, 2016: Journal of Biomolecular Structure & Dynamics
https://www.readbyqxmd.com/read/27897084/multi-spectroscopic-and-molecular-modeling-studies-of-interaction-between-two-different-angiotensin-i-converting-enzyme-inhibitory-peptides-from-gluten-hydrolysate-and-human-serum-albumin
#12
Reza Assaran Darban, Behzad Shareghi, Ahmad Asoodeh, Jamshidkhan Chamani
The present study was carried out to characterize ACE inhibitory peptides which are release from the trypsin hydrolysate of wheat gluten protein. The binding of two inhibitory peptide (P4 and P6) to human serum albumin (HSA) under physiological conditions has been investigated by multi- spectroscopic in combination with molecular modeling techniques. Time-resolved and quenching fluorescence spectroscopies results revealed that the quenching of HSA fluorescence by P4 and P6 in the binary and ternary systems caused HSA-peptides complexes formation...
November 29, 2016: Journal of Biomolecular Structure & Dynamics
https://www.readbyqxmd.com/read/27895586/weakening-impact-of-excessive-human-serum-albumin-ehsa-on-cisplatin-and-etoposide-anticancer-effect-in-c57bl-6-mice-with-tumor-and-in-human-nsclc-a549-cells
#13
Zhen Yang, Ting Zhou, Yuanchi Cheng, Mingming Li, Xianglin Tan, Feng Xu
Excessive human serum albumin (eHSA) impact on anticancer effects is inconsistent. We explored the outcome of cisplatin (DDP)/etoposide (VP-16) plus eHSA in vivo and in vitro. C57BL/6 mice with tumor were used to compare the efficacy of DDP/VP-16 alone and DDP/VP-16+eHSA. Blood albumin was measured to confirm whether eHSA elevate its level. Western blotting assay were used to measure the expression of ERCC1/TOP2A in tumor tissues. Cell proliferation, mRNA, and protein expression of ERCC1/TOP2A were also assayed to compare two groups in A549 cells...
2016: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/27895531/follicular-fluid-biomarkers-for-human-in-vitro-fertilization-outcome-proof-of-principle
#14
Fang Chen, Carl Spiessens, Thomas D'Hooghe, Karen Peeraer, Sebastien Carpentier
BACKGROUND: Human follicular fluid (FF) is a unique biological fluid in which the oocyte develops in vivo, and presents an optimal source for non-invasive biochemical predictors. Oocyte quality directly influences the embryo development and hence, may be used as a predictor of embryo quality. Peptide profiling of FF and its potential use as a biomarker for oocyte quality has never been reported. METHODS: This study screened FF for peptide biomarkers that predict the outcome of in vitro fertilization (IVF)...
2016: Proteome Science
https://www.readbyqxmd.com/read/27892818/interaction-of-stattic-a-stat3-inhibitor-with-human-serum-albumin-spectroscopic-and-computational-study
#15
Ida Syazwani M Affandi, Wei Qi Lee, Shevin R Feroz, Saharuddin B Mohamad, Saad Tayyab
No abstract text is available yet for this article.
November 28, 2016: Journal of Biomolecular Structure & Dynamics
https://www.readbyqxmd.com/read/27892586/bovine-serum-albumin-coated-nanoparticles-for-in-vitro-activated-fluorescence
#16
Mary K Burdette, Ragini Jenkins, Yuriy Bandera, Rhonda R Powell, Terri F Bruce, Xi Yang, Yanzhang Wei, Stephen H Foulger
A fluorophore modified nanoparticle was developed that can only fluoresce when a specific environmental parameter interacts with the system. The model system consisted of an azide modified bovine serum albumin (azBSA) that had been covalently attached to an alkyne modified silicon phthalocyanine (alSiPc) derivative through a copper catalyzed azide/alkyne Huisgen cycloaddition (click reaction). The azBSA/alSiPc assembly was then clicked to a ca. 67 nm poly(propargyl acrylate) (PA) nanoparticle (PA/azBSA/alSiPc)...
November 28, 2016: Nanoscale
https://www.readbyqxmd.com/read/27890435/treating-hypoalbuminemia
#17
REVIEW
Bobbi J Conner
Hypoalbuminemia is common and associated with a variety of disease processes, including those leading to systemic inflammatory response syndrome, gastrointestinal disorders, hepatic disorders, and glomerular diseases. Some animals develop clinical signs directly caused by low serum albumin concentration. There is strong evidence that hypoalbuminemia is associated with worse outcomes; however, evidence justifying albumin supplementation is lacking. Severe adverse events are frequently reported with administration of human serum albumin and there is little evidence of benefit from other products...
November 24, 2016: Veterinary Clinics of North America. Small Animal Practice
https://www.readbyqxmd.com/read/27889668/drug-protein-binding-of-danhong-injection-and-the-potential-influence-of-drug-combination-with-aspirin-insight-by-ultrafiltration-lc-ms-and-molecular-modeling
#18
Junfeng Zhu, Xiaojiao Yi, Peng Huang, Shuqing Chen, Yongjiang Wu
Danhong injection (DHI) is a widely used Chinese medicine injection (CMI) for the clinical treatment of cardiovascular and cerebrovascular diseases. In this study, a simple and efficient in vitro method based on ultrafiltration LC-MS and molecular modeling has been developed to study the human serum albumin (HSA) binding of the compounds in DHI. Seven major components including protocatechuic aldehyde, p-coumaric acid, salvianolic acid D, rosmarinic acid, salvianolic acid E, lithospermic acid and salvianolic acid B were identified as HSA ligands and their binding degrees in the proposed non-saturated model were 26...
November 18, 2016: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/27886774/analysis-of-tricyclic-antidepressants-in-human-plasma-using-online-restricted-access-molecularly-imprinted-solid-phase-extraction-followed-by-direct-mass-spectrometry-identification-quantification
#19
Mariane Gonçalves Santos, Isabela Maria Campos Tavares, Adriano Francisco Barbosa, Jefferson Bettini, Eduardo Costa Figueiredo
The use of a new class of hybrid materials, called restricted access molecularly imprinted polymers (RAMIPs) seems to present a good strategy for the sample preparation of complex matrices, since these materials combine good protein elimination capacity with high degree selectivity. Mass spectrometers (MS) have been successfully used for polar drug identification and quantification. In order to combine the advantages of both RAMIPs and mass spectrometry, we proposed a study that joins these properties in a single system, where we could analyse tricyclic antidepressants from human plasma, without offline extraction or chromatographic separation...
January 15, 2017: Talanta
https://www.readbyqxmd.com/read/27885867/protein-corona-modulation-of-hepatocyte-uptake-and-molecular-mechanisms-of-gold-nanoparticle-toxicity
#20
Kyoungju Choi, Jim E Riviere, Nancy A Monteiro-Riviere
Protein corona formation over gold nanoparticles (AuNP) can modulate cellular responses by altering AuNP physicochemical properties. The liver plays an essential role in metabolism, detoxification and elimination of xenobiotics and drugs as well as circulating NP clearance. We investigated human hepatic uptake of 40 and 80nm AuNP with branched polyethylenimine (BPEI), lipoic acid (LA) and polyethylene glycol (PEG) coatings as well as human plasma protein (HP) and human serum albumin (HSA) coronas. AuNP-mediated cytotoxicity, reactive oxygen/reactive nitrogen species (ROS/RNS), and CYP activity in human hepatocytes as well as molecular mechanisms with 40nm bare and HP BPEI-AuNP were investigated...
November 25, 2016: Nanotoxicology
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