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Human serum albumin

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https://www.readbyqxmd.com/read/28080064/collective-excitations-in-protein-as-a-measure-of-balance-between-its-softness-and-rigidity
#1
Utsab Raj Shrestha, Debsindhu Bhowmik, Kurt William Van Delinder, Eugene Mamontov, Hugh M O'Neill, Qiu Zhang, Ahmet Alatas, Xiang-Qiang Chu
In this article, we elucidate the protein activity from the perspective of protein softness and flexibility by studying the collective phonon-like excitations in a globular protein, human serum albumin (HSA) and taking advantage of the state-of-the-art inelastic X-ray scattering (IXS) technique. Such excitations demonstrate that the protein becomes softer upon thermal denaturation due to disruption of weak non-covalent bonds. On the other hand, no significant change in the local excitations is detected in ligand- (drugs) bound HSA compared to the ligand-free HSA...
January 12, 2017: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/28078915/synthesis-and-characterization-of-thermosensitive-poly-n-vinylcaprolactam-g-collagen
#2
Serap Durkut, Yaşar Murat Elçin
In this study, we synthesized poly(N-vinylcaprolactam)-g-collagen (PNVCL-g-Col) by grafting collagen with carboxyl group-terminated thermosensitive PNVCL-COOH. The resulting biopolymer was evaluated for its structural, thermal, and rheological properties. Aqueous solutions of PNVCL-g-Col exhibited a temperature-dependent phase transition around human physiological temperature (at ∼38.5 °C), and temperature-dependent tunability. PNVCL-g-Col exhibited temperature-dependent release of the model drugs, lidocaine hydrochloride and bovine serum albumin...
January 12, 2017: Artificial Cells, Nanomedicine, and Biotechnology
https://www.readbyqxmd.com/read/28078416/poly-ethylene-glycol-functionalization-of-monolithic-poly-divinyl-benzene-for-improved-miniaturized-solid-phase-extraction-of-protein-rich-samples
#3
Esme Candish, Aminreza Khodabandeh, Marianne Gaborieau, Thomas Rodemann, Robert A Shellie, Andrew A Gooley, Emily F Hilder
Non-specific protein adsorption on hydrophobic solid phase extraction (SPE) adsorbents can reduce the efficacy of purification. To improve sample clean-up, poly(divinyl benzene) (PDVB) monoliths grafted with hydrophilic polyethylene glycol methacrylate (PEGMA) were developed. Residual vinyl groups (RVGs) of the PDVB were employed as anchor points for PEGMA grafting. Two PEGMA monomers, M n 360 and 950, were compared for graft solutions containing 5-20% monomer. Protein binding was qualitatively screened using fluorescently labeled human serum albumin (HSA) to determine optimal PEGMA concentration...
January 11, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28076823/guazuma-ulmifolia-bark-synthesized-ag-au-and-ag-au-alloy-nanoparticles-photocatalytic-potential-dna-protein-interactions-anticancer-activity-and-toxicity-against-14-species-of-microbial-pathogens
#4
Viswanathan Karthika, Ayyakannu Arumugam, Kasi Gopinath, Periyannan Kaleeswarran, Marimuthu Govindarajan, Naiyf S Alharbi, Shine Kadaikunnan, Jamal M Khaled, Giovanni Benelli
In the present study, we focused on a quick and green method to fabricate Ag, Au and Ag/Au alloy nanoparticles (NPs) using the bark extract of Guazuma ulmifolia L. Green synthesized metal NPs were characterized using different techniques, including UV-Vis spectroscopy, FT-IR, XRD, AFM and HR-TEM analyses. The production of Ag, Au and Ag/Au alloy NPs was observed monitoring color change from colorless to brown, followed by pink and dark brown, as confirmed by UV-Vis spectroscopy characteristic peaks at 436, 522 and 510nm, respectively...
January 4, 2017: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/28075404/advanced-oxidation-protein-products-modified-albumin-induces-differentiation-of-raw264-7-macrophages-into-dendritic-like-cells-which-is-modulated-by-cell-surface-thiols
#5
Silvano Garibaldi, Chiara Barisione, Barbara Marengo, Pietro Ameri, Claudio Brunelli, Manrico Balbi, Giorgio Ghigliotti
Local accumulation of Advanced Oxidation Protein Products (AOPP) induces pro-inflammatory and pro-fibrotic processes in kidneys and is an independent predictor of renal fibrosis and of rapid decline of eGFR in patients with chronic kidney disease (CKD). In addition to kidney damage, circulating AOPP may be regarded as mediators of systemic oxidative stress and, in this capacity, they might play a role in the progression of atherosclerotic damage of arterial walls. Atherosclerosis is a chronic inflammatory disease that involves activation of innate and adaptive immunity...
January 10, 2017: Toxins
https://www.readbyqxmd.com/read/28071855/insights-into-the-interaction-of-methotrexate-and-human-serum-albumin-a-spectroscopic-and-molecular-modeling-approach
#6
Li-Yang Cheng, Min Fang, Ai-Min Bai, Yu Ouyang, Yan-Jun Hu
In this study, fluorescence spectroscopy and molecular modeling approaches were employed to investigate the binding of methotrexate to human serum albumin (HSA) under physiological conditions. From the mechanism, it was demonstrated that fluorescence quenching of HSA by methotrexate results from the formation of a methotrexate/HSA complex. Binding parameters calculated using the Stern-Volmer method and the Scatchard method showed that methotrexate binds to HSA with binding affinities in the order 10(4)  L·mol(-1) ...
January 10, 2017: Luminescence: the Journal of Biological and Chemical Luminescence
https://www.readbyqxmd.com/read/28071831/protein-metalation-by-anticancer-metallodrugs-a-joint-esi-ms-and-xrd-investigative-strategy
#7
Antonello Merlino, Tiziano Marzo, Luigi Messori
Interactions of metal based drugs with proteins and consequent adduct formation (the so called "protein metalation" process) play a key role in the mode of action of several anticancer agents and in determining their toxicological profile. Through a novel investigative strategy grounded on the combined use of electrospray ionization mass spectrometry (ESI MS) and biological macromolecule X-ray crystallography we show that it is possible to clarify in depth the metalation process of small model proteins; a number of instructive examples are provided...
January 10, 2017: Chemistry: a European Journal
https://www.readbyqxmd.com/read/28070779/hydrolytic-activity-of-esterase-antibody-complexes-retained-within-gel-capsules-after-complex-isolation
#8
Youji Shimazaki, Rino Miyatsuka
Delipidation in biological samples is important for some diagnostic tests and protein analyses. Lipids in the samples can be hydrolyzed by native esterases (ESs) within gel capsules after ES, and ES-antibody complexes are specifically trapped, extracted, and separated. Acrylamide and agarose gel capsules containing complexes of ES antibody were produced after the complexes were extracted using protein A-immobilized membranes, separated by non-denaturing electrophoresis, and stained by colloidal silver using glucose as a reductant...
January 9, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/28067039/relighting-photosensitizers-by-synergistic-integration-of-albumin-and-perfluorocarbon-for-enhanced-photodynamic-therapy
#9
Hao Ren, Jiaqi Liu, Fenhong Su, Sizhan Ge, Ahu Yuan, Weimin Dai, Jinhui Wu, Yiqiao Hu
Photodynamic therapy (PDT) is hampered by poor water solubility and skin phototoxicity of photosensitizers (PSs). Incorporation of PSs into nanocarrier (Nano-PDT) has been designed to overcome these problems. However, self-quenching of PSs highly condensed in Nano-PDT significantly reduced singlet oxygen (1O2) generation, resulting in unsatisfactory PDT efficacy. Here, we developed a novel tripleffect Nano-PDT, which has a special core-shell nanostructure by synergistic integration of perfluorotributylamine (PFTBA) and human serum albumin (HSA) to improve PDT...
January 9, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28064094/influence-of-core-and-maltose-surface-modification-of-peis-on-their-interaction-with-plasma-proteins-human-serum-albumin-and-lysozyme
#10
Dominika Wrobel, Monika Marcinkowska, Anna Janaszewska, Dietmar Appelhans, Brigitte Voit, Barbara Klajnert-Maculewicz, Maria Bryszewska, Marcel Štofik, Regina Herma, Piotr Duchnowicz, Jan Maly
Regardless of the route of administration, some or all of a therapeutic agent will appear in the blood stream, where it can act on blood cells and other components of the plasma. Recently we have shown that poly(ethylene imines) (PEIs) which interact with plasma proteins are taken up into erythrocyte membranes. These observations led us to investigate the interactions between maltose functionalized hyperbranched PEIs (PEI-Mal) and plasma proteins. Two model proteins were chosen - human serum albumin (HSA) (albumins constitute ∼60% of all plasma proteins), and lysozyme...
January 2, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28063188/utility-of-urinary-tubular-markers-for-monitoring-chronic-tubulointerstitial-injury-after-ischemia-reperfusion
#11
Daisuke Ichikawa, Atsuko Kamijo-Ikemori, Takeshi Sugaya, Keiichi Ohata, Mikako Hisamichi, Seiko Hoshino, Kenjiro Kimura, Yugo Shibagaki
BACKGROUND: The aim of this study was to elucidate whether urinary tubular markers during the chronic phase of acute kidney injury (AKI) are associated with chronic tubulointerstitial damage. METHODS: Male human L-type fatty acid binding protein (L-FABP) chromosomal transgenic (Tg) mice underwent ischemic reperfusion (I/R) injury via renal pedicle clamping for either 10 min or 20 min. Contralateral nephrectomy was performed at the time of tissue reperfusion...
January 7, 2017: Nephrology
https://www.readbyqxmd.com/read/28062376/a-biotin-enrichment-strategy-identifies-novel-carbonylated-amino-acids-in-proteins-from-human-plasma
#12
Jesper F Havelund, Katarzyna Wojdyla, Michael J Davies, Ole N Jensen, Ian Max Møller, Adelina Rogowska-Wrzesinska
: Protein carbonylation is an irreversible protein oxidation correlated with oxidative stress, various diseases and ageing. Here we describe a peptide-centric approach for identification and characterisation of up to 14 different types of carbonylated amino acids in proteins. The modified residues are derivatised with biotin-hydrazide, enriched and characterised by tandem mass spectrometry. The strength of the method lies in an improved elution of biotinylated peptides from monomeric avidin resin using hot water (95°C) and increased sensitivity achieved by reduction of analyte losses during sample preparation and chromatography...
January 3, 2017: Journal of Proteomics
https://www.readbyqxmd.com/read/28059439/comparison-of-three-methods-for-analyzing-loureirin-b-and-human-serum-albumin-interaction-using-capillary-electrophoresis
#13
Yuelin Zhang, Yijie Sha, Kai Qian, Xu Chen, Qin Chen
LB, a bioactive drug, is widely used in treatment of biological diseases. However, due to its poor solution, it is important to find the approach which helps LB to specific biological targets. As the most abundant protein in plasma, HSA plays the role of a carrier of numerous drug ligand. Thus, the interaction between loureirin B (LB) with human serum albumin (HSA) were explored by affinity capillary electrophoresis (ACE), capillary electrophoresis frontal analysis (FA-CE) and pressure-mediated affinity capillary electrophoresis (P-ACE) under simulated physiological conditions (pH = 7...
January 6, 2017: Electrophoresis
https://www.readbyqxmd.com/read/28056175/interaction-of-tissue-engineering-substrates-with-serum-proteins-and-its-influence-on-human-primary-endothelial-cells
#14
Tamilselvan Mohan, Katrin Niegelhell, Chandran Nagaraj, David Reishofer, Stefan Spirk, Andrea Olschewski, Karin Stana Kleinschek, Rupert Johann Kargl
Polymer based biomaterials particularly polycaprolactone (PCL) are one of the most promising substrates for tissue engineering. The surface chemistry of these materials plays a major role since it governs protein adsorption, cell adhesion, viability, degradation and biocompatibility in the first place. This study correlates the interaction of the most abundant serum proteins (albumin, immunoglobulins, fibrinogen) with the surface properties of PCL and its influence on the morphology and metabolic activity of primary human arterial endothelial cells that are seeded on the materials...
January 5, 2017: Biomacromolecules
https://www.readbyqxmd.com/read/28055308/binding-to-ocular-albumin-as-a-half-life-extension-principle-for-intravitreally-injected-drugs-evidence-from-mechanistic-rat-and-rabbit-studies
#15
Holger Fuchs, Frederik Igney
PURPOSE: The ocular half-life of intravitreally (IVT) injected drugs is of major relevance for the suitability of a drug intended for chronic intraocular treatment, as the half-life determines the dosing frequency. Thus, half-life extension principles are very attractive as they can reduce the IVT dosing frequency. In this study, we investigated the ocular pharmacokinetics (PK) of the IVT injected Nanobody(®) BI-X and whether the noncovalent binding of BI-X to vitreous albumin could increase its ocular half-life...
January 5, 2017: Journal of Ocular Pharmacology and Therapeutics
https://www.readbyqxmd.com/read/28053527/fabrication-of-nonfouling-bactericidal-and-bacteria-corpse-release-multifunctional-surface-through-surface-initiated-raft-polymerization
#16
Bailiang Wang, Zi Ye, Yihong Tang, Yuemei Han, Quankui Lin, Huihua Liu, Hao Chen, Kaihui Nan
Infections after surgery or endophthalmitis are potentially blinding complications caused by bacterial adhesion and subsequent biofilm formation on the intraocular lens. Neither single-function anti-adhesion surface nor contacting killing surface can exhibit ideal antibacterial function. In this work, a novel (2-(dimethylamino)-ethyl methacrylate-co-2-methacryloyloxyethyl phosphorylcholine) (p (DMAEMA-co-MPC)) brush was synthesized by "grafting from" method through reversible-addition fragmentation chain transfer polymerization...
2017: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/28048940/towards-understanding-the-molecular-recognition-of-albumin-by-p53-activating-stapled-peptide-atsp-7041
#17
Garima Tiwari, Chandra S Verma
Reactivation of tumor suppressing activity of p53 protein by targeting its negative regulator MDM2/MDMX has been pursued as a potential anticancer strategy. A promising dual inhibitor of MDM2/MDMX that has been developed and is currently in clinical trials is the stapled-peptide ATSP-7041. The activity of this molecule is reported to be modulated in the presence of serum. Albumin is the most abundant protein in serum and is known to bind reversibly to several molecules. To study this interaction, we develop a protocol combining molecular modeling, docking and simulations...
January 3, 2017: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/28045339/imaging-biodistribution-and-efficacy-evaluation-of-188-re-human-serum-albumin-microspheres-via-intraarterial-route-in-an-orthotopic-hepatoma-model
#18
Liang-Cheng Chen, Ya-Jen Chang, Su-Jung Chen, Wan-Chi Lee, Chih-Hsien Chang, Te-Wei Lee, Jui-Hung Shien
Purpose Liver cancer is the second most common cause of death worldwide. This study was to investigate the SPECT/CT, ultrasound, biodistribution and therapeutic evaluation of (188)Re-human serum albumin microspheres ((188)Re-HSAM) in the GP7TB orthotopic hepatoma rat model. Materials and methods HSAM was labeled with (188)Re by using home-made kit and microwave system. The (188)Re-HSAM was administered via intraarterial route. The in vivo distribution of (188)Re-HSAM was determined by biodistribution analysis and nanoSPECT/CT system...
January 3, 2017: International Journal of Radiation Biology
https://www.readbyqxmd.com/read/28042344/noninvasive-imaging-of-myocardial-inflammation-in-myocarditis-using-68-ga-tagged-mannosylated-human-serum-albumin-positron-emission-tomography
#19
Seung-Pyo Lee, Hyung-Jun Im, Shinae Kang, Seock-Jin Chung, Ye Seul Cho, Hyejeong Kang, Ho Seon Park, Do-Won Hwang, Jun-Bean Park, Jin-Chul Paeng, Gi-Jeong Cheon, Yun-Sang Lee, Jae Min Jeong, Yong-Jin Kim
The diagnosis of myocarditis traditionally relies on invasive endomyocardial biopsy but none of the imaging studies so far are specific for infiltration of the inflammatory cells itself. We synthesized (68)Ga-2-(p-isothiocyanatobenzyl)-1,4,7-triazacyclononane-1,4,7-triacetic acid (NOTA) mannosylated human serum albumin (MSA) by conjugating human serum albumin with mannose, followed by conjugation with NOTA and labeling it with (68)Ga. The efficacy of (68)Ga-NOTA-MSA positron emission tomography (PET) for imaging myocardial inflammation was tested in a rat myocarditis model...
2017: Theranostics
https://www.readbyqxmd.com/read/28039847/environment-sensitive-fluorescent-analogue-of-biologically-active-oxazoles-differentially-recognizes-human-serum-albumin-and-bovine-serum-albumin-photophysical-and-molecular-modeling-studies
#20
Jyotirmay Maiti, Suman Biswas, Ankur Chaudhuri, Sandipan Chakraborty, Sibani Chakraborty, Ranjan Das
An environment sensitive fluorophore, 4-(5-(4-(dimethylamino)phenyl)oxazol-2-yl)benzoic acid (DMOBA), that closely mimics biologically active 2,5-disubstituited oxazoles has been designed to probe two homologous serum proteins, human serum albumin (HSA) and bovine serum albumin (BSA) by means of photophysical and molecular modeling studies. This fluorescent analogue exhibits solvent polarity sensitive fluorescence due to an intramolecular charge transfer in the excited state. In comparison to water, the steady state emission spectra of DMOBA in BSA is characterized by a greater blue shift (~10nm) and smaller Stokes' shift (~5980cm(-1)) in BSA than HSA (Stokes'shift~6600cm(-1)), indicating less polar and more hydrophobic environment of the dye in the former than the latter...
December 21, 2016: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
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