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https://www.readbyqxmd.com/read/29027077/optimization-of-dendritic-cell-mediated-cytotoxic-t-cell-activation-by-tracking-of-dendritic-cell-migration-using-reporter-gene-imaging
#1
Hongje Lee, Ho Won Lee, You La Lee, Yong Hyun Jeon, Shin Young Jeong, Sang-Woo Lee, Jaetae Lee, Byeong-Cheol Ahn
PURPOSE: The aim of this study is to optimize the dendritic cell (DC)-mediated T-cell activation using reporter gene imaging and flow cytometric analysis in living mice. PROCEDURES: A murine dendritic cell line (DC2.4) co-expressing effluc and Thy1.1 genes were established by transfection with retroviral vectors. Thy1.1 positive cells were sorted by magnetic bead separation system (DC2.4/effluc). Cell proliferation assay and phenotype analysis to determine the effects of gene transduction on the function of dendritic cells between parental DC2...
October 12, 2017: Molecular Imaging and Biology: MIB: the Official Publication of the Academy of Molecular Imaging
https://www.readbyqxmd.com/read/28978449/quantitative-deformability-cytometry-rapid-calibrated-measurements-of-cell-mechanical-properties
#2
Kendra D Nyberg, Kenneth H Hu, Sara H Kleinman, Damir B Khismatullin, Manish J Butte, Amy C Rowat
Advances in methods that determine cell mechanical phenotype, or mechanotype, have demonstrated the utility of biophysical markers in clinical and research applications ranging from cancer diagnosis to stem cell enrichment. Here, we introduce quantitative deformability cytometry (q-DC), a method for rapid, calibrated, single-cell mechanotyping. We track changes in cell shape as cells deform into microfluidic constrictions, and we calibrate the mechanical stresses using gel beads. We observe that time-dependent strain follows power-law rheology, enabling single-cell measurements of apparent elastic modulus, Ea, and power-law exponent, β...
October 3, 2017: Biophysical Journal
https://www.readbyqxmd.com/read/28967606/differentiation-of-human-dendritic-cell-subsets-for-immune-tolerance-induction
#3
N Deluce-Kakwata-Nkor, L Lamendour, V Chabot, A Héraud, Z Ivanovic, F Halary, F Dehaut, F Velge-Roussel
OBJECTIVES: Since no further progress was achieved, in order to improve the long-term organ transplantation outcome, the immune tolerance appears as an interesting therapeutic goal. Dendritic cells (DCs) are specialized cells participating in the homeostasis of the immune response. Moreover, subsets of DCs, identified in humans, appear to have their respective competences in immune response modulation. Our objective is to purify from PBMC or to differentiate DC subsets from monocytes using several strategies and evaluate their IL10 secretion...
September 26, 2017: Transfusion Clinique et Biologique: Journal de la Société Française de Transfusion Sanguine
https://www.readbyqxmd.com/read/28923503/in-vivo-confocal-microscopy-detects-bilateral-changes-of-corneal-immune-cells-and-nerves-in-unilateral-herpes-zoster-ophthalmicus
#4
Bernardo M Cavalcanti, Andrea Cruzat, Afsun Sahin, Deborah Pavan-Langston, Eric Samayoa, Pedram Hamrah
PURPOSE: To analyze bilateral corneal immune cell and nerve alterations in patients with unilateral herpes zoster ophthalmicus (HZO) by laser in vivo confocal microscopy (IVCM) and their correlation with corneal sensation and clinical findings. MATERIALS AND METHODS: This is a prospective, cross-sectional, controlled single-center study. Twenty-four eyes of 24 HZO patients and their contralateral clinically unaffected eyes and normal controls (n = 24) were included...
September 15, 2017: Ocular Surface
https://www.readbyqxmd.com/read/28865229/cytokine-profiles-of-dendritic-cells-dcs-during-infection-with-bovine-leukaemia-virus-blv
#5
E Iwan, M Szczotka, J Kocki
BLV is an agent of enzootic bovine leukaemia (EBL), an infectious disease affecting cattle worldwide. BLV infection has been associated with immune system disorders and discrepancies in the cytokine network. The significance of dendritic cells in the pathogenesis of BLV infection is largely unknown, but considering their fundamental role in immune response it may be crucial. DCs precursors were isolated with the use of immunomagnetic beads from BLV-infected and BLV-free cows. From these precursors cultures of monocyte derived dendritic cells (MoDCs) were generated with the use of a cytokine cocktail (IL-4 and GM-CSF)...
March 1, 2017: Polish Journal of Veterinary Sciences
https://www.readbyqxmd.com/read/28791124/combining-dendritic-cells-and-b-cells-for-presentation-of-oxidised-tumour-antigens-to-cd8-t-cells
#6
Melanie L Grant, Nicholas Shields, Silke Neumann, Katrin Kramer, Andrea Bonato, Christopher Jackson, Margaret A Baird, Sarah L Young
The dendritic cell (DC) is the foremost antigen-presenting cell (APC) for ex vivo expansion of tumour-specific patient T cells. Despite marked responses in some patients following reinfusion of DC-activated autologous or HLA-matched donor T cells, overall response rates remain modest in solid tumours. Furthermore, most studies aim to generate immune responses against defined tumour-associated antigens (TAA), however, meta-analysis reveals that those approaches have less clinical success than those using whole tumour cells or their components...
July 2017: Clinical & Translational Immunology
https://www.readbyqxmd.com/read/28774159/comparison-of-changes-in-the-interstitial-cells-of-cajal-and-neuronal-nitric-oxide-synthase-positive-neuronal-cells-with-aging-between-the-ascending-and-descending-colon-of-f344-rats
#7
Sun Min Lee, Nayoung Kim, Hyun Jin Jo, Ji Hyun Park, Ryoung Hee Nam, Hye Seung Lee, Hyun Jin Kim, Moon Young Lee, Yong Sung Kim, Dong Ho Lee
Background/Aims: Neuronal degeneration and changes in interstitial cells of Cajal (ICCs) are important mechanisms of age-related constipation. This study aims to compare the distribution of ICCs and neuronal nitric oxide synthase (nNOS) with regard to age-related changes between the ascending colon (AC) and descending colon (DC) in 6-, 31-, and 74-week old and 2-year old male Fischer-344 rats. Methods: The amount of fecal pellet and the bead expulsion times were measured...
October 30, 2017: Journal of Neurogastroenterology and Motility
https://www.readbyqxmd.com/read/28733242/dual-centrifugation-a-new-technique-for-nanomilling-of-poorly-soluble-drugs-and-formulation-screening-by-an-doe-approach
#8
Martin Hagedorn, Ansgar Bögershausen, Matthias Rischer, Rolf Schubert, Ulrich Massing
The development of nanosuspensions of poorly soluble APIs takes a lot of time and high amount of active material is needed. In this publication the use of dual centrifugation (DC) for an effective and rapid API-nanomilling is described for the first time. DC differs from normal centrifugation by an additional rotation of the samples during centrifugation, resulting in a very fast and powerful movement of the samples inside the vials, which - in combination with milling beads - result in effective milling. DC-nanomilling was compared to conventional wet ball milling and results in same or even smaller particle sizes...
July 18, 2017: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/28719632/viscum-album-neutralizes-tumor-induced-immunosuppression-in-a-human-in-vitro-cell-model
#9
Carmen Steinborn, Amy Marisa Klemd, Ann-Sophie Sanchez-Campillo, Sophie Rieger, Marieke Scheffen, Barbara Sauer, Manuel Garcia-Käufer, Konrad Urech, Marie Follo, Annekathrin Ücker, Gunver Sophia Kienle, Roman Huber, Carsten Gründemann
Tumor cells have the capacity to secrete immunosuppressive substances in order to diminish dendritic cell (DC) activity and thereby escape from immune responses. The impact of mistletoe (Viscum album) extracts (VAE), which are frequently used as an additive anti-cancer therapy to stimulate the immune response, is still unknown. Using a human cellular system, the impact of two different VAE (VAEA + VAEI) on the maturation of human dendritic cells and on T cell function has been investigated using flow cytometry, automated fluorescence microscopy and cytokine bead array assays...
2017: PloS One
https://www.readbyqxmd.com/read/28679130/efficacy-of-2-month-treatment-with-cord-blood-serum-eye-drops-in-ocular-surface-disease-an-in-vivo-confocal-microscopy-study
#10
Giuseppe Giannaccare, Marina Buzzi, Michela Fresina, Claudio Velati, Piera Versura
PURPOSE: To investigate the morphological changes of corneal epithelium and subbasal nerves by in vivo confocal microscopy in patients with ocular surface disease (OSD) treated with cord blood serum (CBS) eye drops. METHODS: Twenty patients with OSD (mean age 61.1 ± 12.6 years) were included in this prospective 1-arm study and treated with CBS eye drops for 2 months. Corneal sensitivity, Schirmer test score, breakup time, subjective symptoms [Ocular Surface Disease Index (OSDI) and Visual Analogue Scale (VAS)], and corneal staining were evaluated before (T0) and after (T1) treatment...
August 2017: Cornea
https://www.readbyqxmd.com/read/28239619/evaluation-of-cross-presentation-in-bone-marrow-derived-dendritic-cells-in-vitro-and-splenic-dendritic-cells-ex-vivo-using-antigen-coated-beads
#11
Andrés Alloatti, Fiorella Kotsias, Eik Hoffmann, Sebastian Amigorena
Antigen presentation by MHC class I molecules, also referred to as cross-presentation, elicits cytotoxic immune responses. In particular, dendritic cells (DC) are the most proficient cross-presenting cells, since they have developed unique means to control phagocytic and degradative pathways. This protocol allows the evaluation of antigen cross-presentation both in vitro (by using bone marrow-derived DC) and ex vivo (by purifying CD8(+) DC from spleen after incorporation of particulate antigen) using ovalbumin (OVA)-coupled particles...
November 20, 2016: Bio-protocol
https://www.readbyqxmd.com/read/28224188/an-attempt-to-shorten-loading-time-of-epirubicin-into-dc-beads-%C3%A2-using-vibration-and-a-sieve
#12
Akinaga Sonoda, Norihisa Nitta, Takefumi Yamamoto, Yuki Tomozawa, Shinichi Ohta, Shobu Watanabe, Kiyoshi Murata
PURPOSE: We investigated the possibility of shortening the time required for loading epirubicin into calibrated polyvinyl alcohol-based hydrogel beads (DC Beads(®)) to be used for transarterial chemoembolization. METHOD: After separating the beads suspended in phosphate-buffered saline (PBS) solution by the use of a sieve (clearance 75 µm), epirubicin hydrochloride (EH) was loaded for 20, 30, or 60 s under vibration into DC beads. The EH loading rate into conventionally prepared (control) beads, i...
April 2017: Cardiovascular and Interventional Radiology
https://www.readbyqxmd.com/read/28188808/characterization-of-a-novel-intrinsically-radiopaque-drug-eluting-bead-for-image-guided-therapy-dc-bead-lumi%C3%A2
#13
Koorosh Ashrafi, Yiqing Tang, Hugh Britton, Orianne Domenge, Delphine Blino, Andrew J Bushby, Kseniya Shuturminska, Mark den Hartog, Alessandro Radaelli, Ayele H Negussie, Andrew S Mikhail, David L Woods, Venkatesh Krishnasamy, Elliot B Levy, Bradford J Wood, Sean L Willis, Matthew R Dreher, Andrew L Lewis
We have developed a straightforward and efficient method of introducing radiopacity into Polyvinyl alcohol (PVA)-2-Acrylamido-2-methylpropane sulfonic acid (AMPS) hydrogel beads (DC Bead™) that are currently used in the clinic to treat liver malignancies. Coupling of 2,3,5-triiodobenzaldehyde to the PVA backbone of pre-formed beads yields a uniformly distributed level of iodine attached throughout the bead structure (~150mg/mL) which is sufficient to be imaged under standard fluoroscopy and computed tomography (CT) imaging modalities used in treatment procedures (DC Bead LUMI™)...
March 28, 2017: Journal of Controlled Release: Official Journal of the Controlled Release Society
https://www.readbyqxmd.com/read/28159551/antibody-mediated-depletion-of-immunosuppressive-factors-from-ovarian-carcinoma-associated-ascites-for-investigation-of-paracrine-versus-autocrine-effects
#14
COMPARATIVE STUDY
E Brencicova, S S Diebold
Many studies seek to explore the impact of extrinsic soluble factors present in serum, interstitial fluids or cell-conditioned media on cells in vitro. A convenient approach to elucidate the effects of a particular factor is its selective neutralization. However, intrinsic production of soluble factors such as cytokines by the cultured cells is common and can have an impact via autocrine mechanisms. The addition of cytokine-specific neutralizing antibodies leads to neutralization of the targeted factors irrespective of their source and affects paracrine and autocrine effects alike...
April 2017: Journal of Immunological Methods
https://www.readbyqxmd.com/read/28132823/preparation-and-characterisation-of-vandetanib-eluting-radiopaque-beads-for-locoregional-treatment-of-hepatic-malignancies
#15
Alice Hagan, Gary J Phillips, Wendy M Macfarlane, Andrew W Lloyd, Peter Czuczman, Andrew L Lewis
Since their introduction around a decade ago, embolic drug-eluting beads (DEBs) have become a well-established treatment option for the locoregional transarterial treatment of hepatic malignancies. Despite this success, the therapy is seen to be limited by the choice of drug and more effective options are therefore being sought. These include the small molecule multi-tyrosine kinase inhibitors (MTKi), which exert an anti-angiogenic and anti-proliferative effect that could be highly beneficial in combating some of the unwanted downstream consequences of embolization...
April 1, 2017: European Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/28113953/towards-high-throughput-cell-growth-screening-a-new-cmos-8-%C3%A3-8-biosensor-array-for-life-science-applications
#16
Ghazal Nabovati, Ebrahim Ghafar-Zadeh, Antoine Letourneau, Mohamad Sawan
In this paper we present a CMOS capacitive sensor array as a compact and low-cost platform for high-throughput cell growth monitoring. The proposed biosensor, consists of an array of 8 × 8 CMOS fully differential charge-based capacitive measurement sensors. A DC-input Σ∆ modulator is used to convert the sensors' signals to digital values for reading out the biological/chemical data and further signal processing. To compensate the mismatch variations between the current mirror transistors, a calibration circuitry is proposed which removes the output voltage offset with less than 8...
April 2017: IEEE Transactions on Biomedical Circuits and Systems
https://www.readbyqxmd.com/read/28026784/kilohertz-bandwidth-dual-stage-haptic-device-lets-you-touch-brownian-motion
#17
Tianming Lu, Cecile Pacoret, David Heriban, Abdenbi Mohand-Ousaid, Stephane Regnier, Vincent Hayward
This paper describes a haptic interface that has a uniform response over the entire human tactile frequency range. Structural mechanics makes it very difficult to implement articulated mechanical systems that can transmit high frequency signals. Here, we separated the frequency range into two frequency bands. The lower band is within the first structural mode of the corresponding haptic device while the higher one can be transmitted accurately by a fast actuator operating from conservation of momentum, that is, without reaction forces to the ground...
July 2017: IEEE Transactions on Haptics
https://www.readbyqxmd.com/read/27904450/survival-of-patients-with-intermediate-stage-hepatocellular-carcinoma-treated-with-superselective-transarterial-chemoembolization-using-doxorubicin-loaded-dc-bead-under-cone-beam-computed-tomography-control
#18
Peter Popovic, Borut Stabuc, Rado Jansa, Manca Garbajs
BACKGROUND: The purpose of this retrospective study was to evaluate treatment response, adverse events and survival rates of patients with intermediate stage HCC treated with superselective doxorubicin-loaded DC Bead transarterial chemoembolization (DEBDOX) under cone beam computed tomography (CBCT) control. PATIENTS AND METHODS: Between October 2010 and June 2012, 35 consecutive patients with intermediate stage HCC (32 male, 3 female; average age, 67.5 ± 7.8 years; 22 patients Child-Pugh class A, 8 class B, 5 without cirrhosis) were treated with DEBDOX TACE...
December 1, 2016: Radiology and Oncology
https://www.readbyqxmd.com/read/27856136/korean-multicenter-registry-of-transcatheter-arterial-chemoembolization-with-drug-eluting-embolic-agents-for-nodular-hepatocellular-carcinomas-six-month-outcome-analysis
#19
MULTICENTER STUDY
Myungsu Lee, Jin Wook Chung, Kwang-Hun Lee, Jong Yun Won, Ho Jong Chun, Han Chu Lee, Jin Hyoung Kim, In Joon Lee, Saebeom Hur, Hyo-Cheol Kim, Yoon Jun Kim, Gyoung Min Kim, Seung-Moon Joo, Jung Suk Oh
PURPOSE: To assess the efficacy and safety of transcatheter arterial chemoembolization with drug-eluting embolic (DEE) agents for nodular hepatocellular carcinoma (HCC). MATERIALS AND METHODS: The study design was a prospective multicenter registry-based, single-arm clinical trial that included 152 patients. One hundred three (67.8%) had a Child-Pugh class/score of A5, 114 (75.0%) had a performance status of 0, and 77 (50.7%) had Barcelona Clinic Liver Cancer (BCLC) stage A disease...
April 2017: Journal of Vascular and Interventional Radiology: JVIR
https://www.readbyqxmd.com/read/27833376/transarterial-chemoembolization-with-drug-eluting-beads-in-hepatocellular-carcinoma
#20
EDITORIAL
Hee Chul Nam, Bohyun Jang, Myeong Jun Song
Transarterial chemoembolization (TACE) is a widely used standard treatment for patients with hepatocellular carcinoma (HCC) who are not suitable candidates for curative treatments. The rationale for TACE is that intra-arterial chemotherapy using lipiodol and chemotherapeutic agents, followed by selective vascular embolization, results in a strong cytotoxic effect as well as ischemia (conventional TACE). Recently, drug-eluting beads (DC Beads(®)) have been developed for transcatheter treatment of HCC to deliver higher doses of the chemotherapeutic agent and to prolong contact time with the tumor...
October 28, 2016: World Journal of Gastroenterology: WJG
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