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ex vivo renal perfusion

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https://www.readbyqxmd.com/read/27911373/the-mouse-isolated-perfused-kidney-technique
#1
Jan Czogalla, Frank Schweda, Johannes Loffing
The mouse isolated perfused kidney (MIPK) is a technique for keeping a mouse kidney under ex vivo conditions perfused and functional for 1 hr. This is a prerequisite for studying the physiology of the isolated organ and for many innovative applications that may be possible in the future, including perfusion decellularization for kidney bioengineering or the administration of anti-rejection or genome-editing drugs in high doses to prime the kidney for transplantation. During the time of the perfusion, the kidney can be manipulated, renal function can be assessed, and various pharmaceuticals administered...
November 17, 2016: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/27895453/a-case-of-a-living-related-kidney-transplantation-after-ex-vivo-repair-of-the-donor-renal-artery-aneurysm
#2
P Christopoulos, A Faryal, M Dosani, D Rix, D Talbot
BACKGROUND: Kidney transplantation is the definite surgical treatment for end-stage renal disease. Shortage of organs and the increasing number of patients with end-stage renal disease has led to an expansion of the selection criteria promoting the use of organs from marginal donors. Use of kidneys with renal artery aneurysm (RAA) is one such example. Description of the case: We report a case of living-related kidney transplantation from a 46-year-old female donor with unilateral RAA to her 68-year-old father...
January 2016: Hippokratia
https://www.readbyqxmd.com/read/27861293/organ-pretreatment-with-cytotopic-endothelial-localizing-peptides-to-ameliorate-microvascular-thrombosis-and-perfusion-deficits-in-ex-vivo-renal-hemoreperfusion-models
#3
Karim Hamaoui, Sally Gowers, Martyn Boutelle, Terry H Cook, George Hanna, Ara Darzi, Richard Smith, Anthony Dorling, Vassilios Papalois
BACKGROUND: Hypothermic machine organ perfusion (HMP) offers opportunity to manipulate grafts with pharmacological agents prior to transplantation. Pretreating organs with novel cytotopic anticoagulant peptides that localize to endothelial cell membranes could ameliorate microvascular thrombotic sequelae posttransplantation. We describe experiments testing thrombalexin (TLN), a novel cell binding thrombin inhibitor, using porcine and unused human kidneys in a series of ex vivo normothermic hemoreperfusion models...
December 2016: Transplantation
https://www.readbyqxmd.com/read/27820833/multiparametric-functional-mri-a-tool-to-uncover-subtle-changes-following-allogeneic-renal-transplantation
#4
Mike Notohamiprodjo, Aivars Kalnins, Martin Andrassy, Manuel Kolb, Benjamin Ehle, Susanna Mueller, Michael N Thomas, Jens Werner, Markus Guba, Konstantin Nikolaou, Joachim Andrassy
PURPOSE: To investigate multiparametric functional MRI to characterize acute rejection in a murine allogeneic renal transplant model and evaluate the effect of novel therapeutics. MATERIAL AND METHODS: We performed allogeneic and syngeneic orthotopic transplantations (Balb/c to C57Bl/6 and C57Bl/6 to C57Bl/6). Allogeneic Groups (n = 5) were either treated with the anti-CCL2-Spiegelmer (mNOX-E36) in monotherapy or in combination with low doses of Ciclosporin-A (10mg/kgBW/d) for 10 days...
2016: PloS One
https://www.readbyqxmd.com/read/27746573/anesthesia-for-bench-surgery
#5
S Sachin, M C Rajesh, E K Ramdas
Surgical removal of the kidney tumor outside the body, (ex vivo renal bench surgery) followed by auto transplantation is an emerging and often done procedure to reconstruct the urinary tract. It possesses immense challenges to both the anesthesiologists and the surgeons. The risks are multiplied if you are performing the surgery on a solitary functioning kidney. Here, we are describing the anesthetic management of 70-year-old male post nephrectomy patient undergoing renal auto transplantation by bench surgery...
September 2016: Anesthesia, Essays and Researches
https://www.readbyqxmd.com/read/27697008/noninvasive-assessment-of-renal-fibrosis-with-magnetization-transfer-mr-imaging-validation-and-evaluation-in-murine-renal-artery-stenosis
#6
Kai Jiang, Christopher M Ferguson, Behzad Ebrahimi, Hui Tang, Timothy L Kline, Tyson A Burningham, Prassana K Mishra, Joseph P Grande, Slobodan I Macura, Lilach O Lerman
Purpose To test the utility of magnetization transfer imaging in detecting and monitoring the progression of renal fibrosis in mice with unilateral renal artery stenosis. Materials and Methods This prospective study was approved by the Institutional Animal Care and Use Committee. Renal artery stenosis surgery (n = 10) or sham surgery (n = 5) was performed, and the stenotic and contralateral kidneys were studied longitudinally in vivo at baseline and 2, 4, and 6 weeks after surgery. After a 16.4-T magnetic resonance imaging examination, magnetization transfer ratio was measured as an index of fibrosis (guided by parameters selected in preliminary phantom studies)...
October 3, 2016: Radiology
https://www.readbyqxmd.com/read/27681269/an-assessment-of-urinary-biomarkers-in-a-series-of-declined-human-kidneys-measured-during-ex-vivo-normothermic-kidney-perfusion
#7
Sarah A Hosgood, Michael L Nicholson
BACKGROUND: The measurement of urinary biomarkers during ex-vivo normothermic kidney perfusion (EVKP) may aid in the assessment of a kidney prior to transplantation. This study measured levels of neutrophil gelatinase-associated lipocalin (NGAL), kidney injury molecule-1 (KIM-1) and endothelin-1 (ET-1) during EVKP in a series of discarded human kidneys. METHODS: Fifty six kidneys from deceased donors were recruited into the study. Each kidney underwent 60 minutes of EVKP and was scored based on the macroscopic appearance, renal blood flow and urine output...
September 27, 2016: Transplantation
https://www.readbyqxmd.com/read/27647696/continuous-normothermic-ex-vivo-kidney-perfusion-is-superior-to-brief-normothermic-perfusion-following-static-cold-storage-in-donation-after-circulatory-death-pig-kidney-transplantation
#8
J M Kaths, J Y Cen, Y M Chun, J Echeverri, I Linares, S Ganesh, P Yip, R John, D Bagli, I Mucsi, A Ghanekar, D R Grant, L A Robinson, M Selzner
Hypothermic preservation is known to cause renal graft injury, especially in donation after circulatory death (DCD) kidney transplantation. We investigated the impact of cold storage (SCS) versus short periods of normothermic ex vivo kidney perfusion (NEVKP) after SCS versus prolonged, continuous NEVKP with near avoidance of SCS on kidney function after transplantation. Following 30 min of warm ischemia, kidneys were removed from 30-kg Yorkshire pigs and preserved for 16 h with (A) 16 h SCS, (B) 15 h SCS + 1 h NEVKP, (C) 8 h SCS + 8 h NEVKP, and (D) 16 h NEVKP...
September 20, 2016: American Journal of Transplantation
https://www.readbyqxmd.com/read/27495754/organ-pretreatment-with-cytotopic-endothelial-localising-peptides-to-ameliorate-microvascular-thrombosis-perfusion-deficits-in-ex-vivo-renal-haemo-reperfusion-models
#9
Karim Hamaoui, Sally Gowers, Martyn Boutelle, Terry Cook, George Hanna, Ara Darzi, Richard Smith, Anthony Dorling, Vassilios Papalois
BACKGROUND: Hypothermic machine organ perfusion (HMP) offers opportunity to manipulate grafts with pharmacological agents prior to transplantation. Pretreating organs with novel cytotopic anti-coagulant peptides that localise to endothelial cell membranes could ameliorate microvascular thrombotic sequelae posttransplantation. We describe experiments testing Thrombalexin (TLN), a novel cell binding thrombin-inhibitor, using porcine and unused human kidneys in a series of ex-vivo normothermic haemo-reperfusion models...
August 5, 2016: Transplantation
https://www.readbyqxmd.com/read/27479157/eight-hour-continuous-normothermic-ex-vivo-kidney-perfusion-is-a-safe-preservation-technique-for-kidney-transplantation-a-new-opportunity-for-the-storage-assessment-and-repair-of-kidney-grafts
#10
J Moritz Kaths, Juan Echeverri, Nicolas Goldaracena, Kristine S Louis, Yi-Min Chun, Ivan Linares, Aryn Wiebe, Daniel B Foltys, Paul M Yip, Rohan John, Istvan Mucsi, Anand Ghanekar, Darius J Bagli, David R Grant, Lisa A Robinson, Markus Selzner
BACKGROUND: Hypothermic kidney storage causes preservation injury and is poorly tolerated by renal grafts. We investigated whether static cold storage (SCS) can be safely replaced with a novel technique of pressure-controlled normothermic ex vivo kidney perfusion (NEVKP) in heart-beating donor kidney transplantation. METHODS: Right kidneys were removed from 30 kg Yorkshire pigs in a model of heart-beating donation and either preserved in cold histidine-tryptophan-ketoglutarate solution for 8 hours (n = 5), or subjected to 8 hours of pressure-controlled NEVKP (n = 5) followed by renal heterotopic autotransplantation...
September 2016: Transplantation
https://www.readbyqxmd.com/read/27467537/continuous-normothermic-ex-vivo-kidney-perfusion-improves-graft-function-in-donation-after-circulatory-death-pig-kidney-transplantation
#11
J Moritz Kaths, Juan Echeverri, Yi Min Chun, Jun Yu Cen, Nicolas Goldaracena, Ivan Linares, Luke S Dingwell, Paul Yip, Rohan John, Darius Bagli, Istvan Mucsi, Anand Ghanekar, David Grant, Lisa Robinson, Markus Selzner
BACKGROUND: Donation after circulatory death (DCD) is current clinical practice to increase the donor pool. Deleterious effects on renal graft function are described for hypothermic preservation. Therefore, current research focuses on investigating alternative preservation techniques such as normothermic perfusion. METHODS: We compared continuous pressure-controlled normothermic ex vivo kidney perfusion (NEVKP) with static cold storage (SCS) in a porcine model of DCD autotransplantation...
July 27, 2016: Transplantation
https://www.readbyqxmd.com/read/27457700/extracellular-k-rapidly-controls-nacl-cotransporter-phosphorylation-in-the-native-distal-convoluted-tubule-by-cl-dependent-and-independent-mechanisms
#12
David Penton, Jan Czogalla, Agnieszka Wengi, Nina Himmerkus, Dominique Loffing-Cueni, Monique Carrel, Renuga Devi Rajaram, Olivier Staub, Markus Bleich, Frank Schweda, Johannes Loffing
KEY POINTS: High dietary potassium (K(+) ) intake dephosphorylates and inactivates the NaCl cotransporter (NCC) in the renal distal convoluted tubule (DCT). Using several ex vivo models, we show that physiological changes in extracellular K(+) , similar to those occurring after a K(+) rich diet, are sufficient to promote a very rapid dephosphorylation of NCC in native DCT cells. Although the increase of NCC phosphorylation upon decreased extracellular K(+) appears to depend on cellular Cl(-) fluxes, the rapid NCC dephosphorylation in response to increased extracellular K(+) is not Cl(-) -dependent...
November 1, 2016: Journal of Physiology
https://www.readbyqxmd.com/read/27273794/successful-transplantation-of-human-kidneys-deemed-untransplantable-but-resuscitated-by-ex-vivo-normothermic-machine-perfusion
#13
S A Hosgood, K Saeb-Parsy, M O Hamed, M L Nicholson
We report the successful transplantation of a pair of human kidneys that were declined for transplantation due to inadequate in situ perfusion but subsequently transplanted after perfusion and assessment using ex vivo normothermic perfusion (EVNP). The kidneys were from a 35-year-old man, a donation after circulatory death donor. Both kidneys were declined by all UK transplant centers. On arrival, the kidneys had significant areas of incomplete clearance of blood from the microcirculation that did not clear after a further attempt to flush them...
November 2016: American Journal of Transplantation
https://www.readbyqxmd.com/read/27145197/ex-vivo-normothermic-perfusion-in-renal-transplantation-past-present-and-future
#14
Cissy Yong, Sarah A Hosgood, Michael L Nicholson
PURPOSE OF REVIEW: Marginal donor kidneys make up a substantial proportion of transplanted kidneys. Nonetheless, they are more susceptible to injury during procurement and preservation. Normothermic perfusion is an alternative method of organ preservation that can be used to improve the quality, resuscitate, assess and potentially repair the organ. This review provides an up-to-date summary of the role of normothermic perfusion in renal transplantation and what we can expect in the future...
June 2016: Current Opinion in Organ Transplantation
https://www.readbyqxmd.com/read/27103404/targeted-dual-modality-imaging-in-renal-cell-carcinoma-an-ex-vivo-kidney-perfusion-study
#15
Marlène C H Hekman, Otto C Boerman, Mirjam de Weijert, Desirée L Bos, Egbert Oosterwijk, Hans F Langenhuijsen, Peter F A Mulders, Mark Rijpkema
PURPOSE: Antibodies labeled with both a near-infrared fluorescent dye and a radionuclide can be used for tumor-targeted intraoperative dual-modality imaging. Girentuximab is a chimeric monoclonal antibody against carbonic anhydrase IX (CAIX), an antigen expressed in 95% of clear cell renal cell carcinoma (ccRCC). This study aimed to assess the feasibility of targeted dual-modality imaging with (111)In-girentuximab-IRDye800CW using ex vivo perfusion of human tumorous kidneys. EXPERIMENTAL DESIGN: Seven radical nephrectomy specimens from patients with ccRCC were perfused during 11 to 15 hours with dual-labeled girentuximab and subsequently rinsed during 2...
September 15, 2016: Clinical Cancer Research: An Official Journal of the American Association for Cancer Research
https://www.readbyqxmd.com/read/27097986/mechanisms-of-hypothermic-machine-perfusion-to-decrease-donation-after-cardiac-death-graft-inflammation-through-the-pathway-of-upregulating-expression-of-klf2-and-inhibiting-tgf-%C3%AE-signaling
#16
Zhongzhong Liu, Zibiao Zhong, Jianan Lan, Mingxia Li, Wei Wang, Jing Yang, Chenwei Tang, Jie Wang, Shaojun Ye, Yan Xiong, Yanfeng Wang, Qifa Ye
Hypothermic machine perfusion (HMP) has been known as an efficient way to improve kidney graft function, but the underlying mechanisms remain unclear. Here, we adopt a rabbit reperfusion mode to investigate the upstream mechanisms of end-ischemic HMP of kidneys from donors after cardiac death (DCD), with static cold storage (CS) as a control. Eighteen New Zealand healthy male rabbits (12 weeks old, with a weight of 3.0 ± 0.2 kg) were randomly divided into three groups: HMP group, CS group, and Normal group (n = 6)...
April 21, 2016: Artificial Organs
https://www.readbyqxmd.com/read/27063863/rate-determining-and-rate-limiting-steps-in-the-clearance-and-excretion-of-a-potent-and-selective-p21-activated-kinase-inhibitor-a-case-study-of-rapid-hepatic-uptake-and-slow-elimination-in-rat
#17
Peter W Fan, Jacob Z Chen, M Allan Jaochico, Hank La, Ning Liu, Teresa Mulder, Robert T Cass, Matthew Durk, Kirsten Messick, Nicole Valle, Shannon Liu, Wendy Lee, James J Crawford, Joachim Rudolf, Lesley J Murray, S Cyrus Khojasteh, Matthew Wright
BACKGROUND: Significant under-prediction of in vivo clearance in rat was observed for a potent p21-activated kinase (PAK1) inhibitor, GNE1. OBJECTIVE: Rate-determining (rapid uptake) and rate-limiting (slow excretion) steps in systemic clearance and elimination of GNE1, respectively, were evaluated to better understand the cause of the in vitro-in vivo (IVIV) disconnect. METHODS: A series of in vivo, ex vivo, and in vitro experiments were carried out: 1) the role of organic cation transporters (Oct or Slc22a) was investigated in transporter knock-out and wild-type animals with or without 1-aminobenzotriazole (ABT) pretreatment; 2) the concentration-dependent hepatic extraction ratio was determined in isolated perfused rat liver; and 3) excreta were collected from both bile duct cannulated and non-cannulated rats after intravenous injection...
2016: Drug Metabolism Letters
https://www.readbyqxmd.com/read/26794829/animal-models-of-light-chain-deposition-disease-provide-a-better-understanding-of-nodular-glomerulosclerosis
#18
Guillermo A Herrera, Elba A Turbat-Herrera, Jiamin Teng
BACKGROUND: Light chain deposition disease (LCDD) is a model of glomerulosclerosis. The mature lesion of LCDD mimics nodular glomerulosclerosis in diabetic nephropathy. The pathogenetic mechanisms involved are similar in both disorders, though the causative factors are entirely different. This fact highlights the generic response of the mesangium to varied stimuli. In-vitro work has provided much insight into the pathogenesis of glomerulosclerosis in LCDD where the mesangium is the main target for initiation and progression of the disease...
2016: Nephron
https://www.readbyqxmd.com/read/26527992/ex-vivo-perfusion-machines-in-kidney-transplantation-the-significance-of-the-resistivity-index
#19
Florin Ioan Elec, Ciprian Lucan, Liviu Ghervan, Valentin Munteanu, Silviu Moga, Mihai Suciu, Dan Enache, Alina Elec, Adriana Munteanu, Adrian Barbos, Gheorghita Iacob, Mihai Lucan
INTRODUCTION: With a growing shortage of organs for transplantation, finding ways of increasing the donor organ pool remains of utmost importance. Perfusion machines (PM) have been proven to enhance the potential for kidney transplants to function sooner, last longer, giving patients the opportunity for a better life quality. OBJECTIVE: The aim of this study is to evaluate the relation between the resistance index provided by the PM, the postoperative resistance index measured by Doppler ultrasound and the initial graft outcome...
2014: Clujul Medical (1957)
https://www.readbyqxmd.com/read/26474973/the-use-of-ex-vivo-normothermic-perfusion-for-the-resuscitation-and-assessment-of-human-kidneys-discarded-because-of-inadequate-in-situ-perfusion
#20
Sarah A Hosgood, A D Barlow, J Dormer, M L Nicholson
BACKGROUND: Many kidneys are rejected for transplantation due to inadequate in situ perfusion during organ retrieval because of the risk of additional ischaemic injury and microvasculature thrombosis. This study describes the use of ex vivo normothermic perfusion (EVNP) for the resuscitation and assessment of human kidneys that were discarded after inadequate in situ perfusion. METHODS: Twenty-two human kidneys were retrieved but then deemed unsuitable for transplantation, primarily due to inadequate in situ perfusion...
2015: Journal of Translational Medicine
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