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https://www.readbyqxmd.com/read/28822000/effects-of-fusariotoxin-co-exposure-on-thp-1-human-immune-cells
#1
Marie-Caroline Smith, Stéphanie Madec, Samuel Troadec, Emmanuel Coton, Nolwenn Hymery
Deoxynivalenol (DON), nivalenol (NIV), T-2 toxin (T2), fumonisin B1 (FB1), zearalenone (ZEA), and moniliformin (MON) mycotoxins are common food and feed contaminants produced by Fusarium spp. However, while they are usually found to co-occur in a large range of commodities, only few data are available on mycotoxin co-exposure effects and cellular response mechanisms. In this study, the individual and combined toxic effects of these fusariotoxins were evaluated on the THP-1 human immune cell line as major fusariotoxins are mostly potent immunomodulators...
August 18, 2017: Cell Biology and Toxicology
https://www.readbyqxmd.com/read/28821308/influence-of-gamma-irradiation-on-porcine-serum-albumin-structural-properties-and-allergenicity
#2
Xudong Zhu, Wei Wang, Juan Shen, Xinglian Xu, Guanghong Zhou
Pork provides an ideal source of food energy; however, pork can elicit an allergic reaction, and porcine serumalbumin (PSA) has been identified as a major allergen. This study examined the impact of gamma irradiation on the allergenicity and structural qualities of PSA; the PSA solution was gamma-irradiated at 1, 2, 4, 6, and 8 kGy. Allergenicity was investigated by immunoblotting and competitive indirect ELISA using serum from patients who are allergic to pork, and conformational changes in irradiated PSA were measured by circular dichroism, sulfhydryl group detection, and fluorescence emission spectra...
August 18, 2017: Journal of AOAC International
https://www.readbyqxmd.com/read/28821140/the-potential-of-cashew-gum-functionalization-as-building-blocks-for-layer-by-layer-films
#3
Álvaro J Leite, Rui R Costa, Ana M S Costa, Jeanny S Maciel, José F G Costa, Regina C M de Paula, João F Mano
Cashew gum (CG), an exudate polysaccharide from Anacardium occidentale trees, was carboxymethylated (CGCm) and oxidized (CGO). These derivatives were characterized by FTIR and zeta potential measurements confirming the success of carboxymethylation and oxidation reactions. Nanostructured multilayered films were then produced through layer-by-layer (LbL) assembly in conjugation with chitosan via electrostatic interactions or Schiff bases covalent bonds. The films were analyzed by QCM-D and AFM. CG functionalization increased the film thickness, with the highest thickness being achieved for the lowest oxidation degree...
October 15, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28820004/drug-induced-liver-injury-in-children-clinical-observations-animal-models-and-regulatory-status
#4
Qiang Shi, Xi Yang, James J Greenhaw, Alec Thomas Salminen, Gary M Russotti, William F Salminen
Drug-induced liver injury in children (cDILI) accounts for about 1% of all reported adverse drug reactions throughout all age groups, less than 10% of all clinical DILI cases, and around 20% of all acute liver failure cases in children. The overall DILI susceptibility in children has been assumed to be lower than in adults. Nevertheless, controversial evidence is emerging about children's sensitivity to DILI, with children's relative susceptibility to DILI appearing to be highly drug-specific. The culprit drugs in cDILI are similar but not identical to DILI in adults (aDILI)...
January 1, 2017: International Journal of Toxicology
https://www.readbyqxmd.com/read/28819142/development-and-inter-laboratory-assessment-of-droplet-digital-pcr-assays-for-multiplex-quantification-of-15-genetically-modified-soybean-lines
#5
Alexandra Bogožalec Košir, Bjørn Spilsberg, Arne Holst-Jensen, Jana Žel, David Dobnik
Quantification of genetically modified organisms (GMOs) in food and feed products is often required for their labelling or for tolerance thresholds. Standard-curve-based simplex quantitative polymerase chain reaction (qPCR) is the prevailing technology, which is often combined with screening analysis. With the rapidly growing number of GMOs on the world market, qPCR analysis becomes laborious and expensive. Innovative cost-effective approaches are therefore urgently needed. Here, we report the development and inter-laboratory assessment of multiplex assays to quantify GMO soybean using droplet digital PCR (ddPCR)...
August 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28819087/investigation-and-characterization-of-%C3%AE-lactam-resistance-in-escherichia-coli-strains-isolated-from-bamboo-rats-rhizomys-sinensis-in-zhejiang-province-china
#6
Hui Zhang, Kun Li, Yajing Wang, Mujeeb Ur Rehman, Yijiang Liu, Junjie Jin, Junping Peng, Fazul Nabi, Khalid Mehmood, Houqiang Luo, Jiaxiang Wang
This study was undertaken to investigate drug resistance in Escherichia coli (E. coli) strains isolated from bamboo rats in Zhejiang province of China. One hundred and fifty-four E. coli strains were isolated from dead bamboo rats. Polymerase chain reaction (PCR) was used to detect the representative genes encoding resistance to commonly used β-lactam antibiotics. Highest resistance was observed for cefradine (24.03%), followed by penicillin (20.78%) and ceftazidime (20.13%). The isolation rates of β-lactam resistance genes were 53...
August 17, 2017: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/28818882/neuropsychiatric-adverse-drug-reactions-in-children-initiated-on-montelukast-in-real-life-practice
#7
Brigitte Benard, Valérie Bastien, Benjamin Vinet, Roger Yang, Maja Krajinovic, Francine M Ducharme
Although montelukast is generally well tolerated, postmarketing studies have reported serious neuropsychiatric adverse drug reactions (ADRs) leading to a United States Food and Drug Administration black box warning. The objective of this study was to determine the incidence of neuropsychiatric ADRs leading to discontinuation of montelukast in asthmatic children.We conducted a retrospective cohort study in children aged 1-17 years initiated on montelukast. In a nested cohort study, children initiated on montelukast as monotherapy or adjunct therapy to inhaled corticosteroids (ICS) were matched to those initiated on ICS monotherapy...
August 2017: European Respiratory Journal: Official Journal of the European Society for Clinical Respiratory Physiology
https://www.readbyqxmd.com/read/28818095/frequency-distribution-and-immunologic-nature-of-infusion-reactions-in-subjects-receiving-pegloticase-for-chronic-refractory-gout
#8
Leonard H Calabrese, Arthur Kavanaugh, Anthony E Yeo, Peter E Lipsky
BACKGROUND: To assess frequency and distribution of infusion reactions (IRs) in responders and nonresponders in randomized clinical trials (RCTs) of intravenous pegloticase and the utility of the National Institute of Allergy and Infectious Disease/Food and Allergy and Anaphylaxis Network (NIAID/FAAN) criteria for identifying anaphylaxis in subjects experiencing IRs. METHODS: IRs from two RCTs of pegloticase were evaluated and categorized as anaphylaxis, hypersensitivity, or other...
August 17, 2017: Arthritis Research & Therapy
https://www.readbyqxmd.com/read/28813779/dna-profiling-of-leuconostoc-citreum-strains-in-fermented-foods-by-repetitive-element-polymerase-chain-reaction
#9
Jasmine Kaur, Anshul Sharma, Sulhee Lee, Young-Seo Park
To identify and discriminate the bacterial species at the sub-specific level, rep-PCR method is a reliable genomic fingerprinting tool. Fourteen strains of bacteria were isolated from different food sources, identified as Leuconostoc citreum using 16S rRNA gene sequencing and amplified using rep-primers (REP, ERIC, and (GTG)5). Fingerprinting patterns generated band range in between 300-6000 bp with REP, 150-6000bp with ERIC and 200-1700 bp with (GTG)5 primers respectively. In UPGMA dendrogram analysis, 14 strains were clustered into three clades (I, II and III) with all the primers, thus differentiating them at the molecular level...
August 17, 2017: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28810380/when-food-becomes-an-obsession-overweight-is-related-to-food-related-obsessive-compulsive-behavior
#10
Katrijn Houben, Anita Jansen
In this study, it was examined whether overweight is associated with food-related obsessions and compulsions. Participants with a healthy weight ( n = 27) and participants who were overweight ( n = 33) filled out the Yale-Brown-Cornell Eating Disorder Scale, the Eating Obsessive-Compulsive Scale, and the Emotional and Behavioral Reactions to Intrusions Questionnaire to assess frequency, distress, control, and reactance associated with food-related preoccupations and compulsions. Overweight participants showed increased food-related preoccupations, compulsive eating, and heightened emotional and behavioral reactance compared to participants with a healthy weight...
January 1, 2017: Journal of Health Psychology
https://www.readbyqxmd.com/read/28809975/discovery-of-synthetic-small-molecules-that-enhance-the-number-of-stomata-c-h-functionalization-chemistry-for-plant-biology
#11
Asraa Ziadi, Naoyuki Uchida, Hiroe Kato, Rina Hisamatsu, Ayato Sato, Shinya Hagihara, Kenichiro Itami, Keiko U Torii
The increasing climate changes and global warming are leading to colossal agricultural problems such as abatement of food production and quality. As stomatal development is considered to play a key role in crop plant productivity and water-use efficiency, studying stomatal development is useful for understanding the productivity of plant systems for both natural and agricultural systems. Herein, we report the first-in-class synthetic small molecules enhancing the number of stomata in Arabidopsis thaliana that have been discovered by screening of the chemical library and further optimized by the Pd-catalyzed C-H arylation reaction...
August 15, 2017: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/28808910/nmr-resonance-assignments-of-a-hypoallergenic-isoform-of-the-major-birch-pollen-allergen-bet-v-1
#12
Linda Ahammer, Sarina Grutsch, Michael Wallner, Fatima Ferreira, Martin Tollinger
In Northern America and Europe a great number of people are suffering from birch pollen allergy and pollen related food allergies. The trigger for these immunological reactions is the 17.5 kDa major birch pollen allergen Bet v 1, which belongs to the family of PR-10 (pathogenesis-related) proteins. In nature, Bet v 1 occurs as a mixture of various isoforms that possess different immunological properties despite their high sequence identities. Bet v 1.0102 (Bet v 1d), which is investigated here, is a hypoallergenic isoform of Bet v 1 and a potential candidate for allergen-specific immunotherapy...
August 14, 2017: Biomolecular NMR Assignments
https://www.readbyqxmd.com/read/28808314/cellular-toxicity-pathways-of-inorganic-and-methyl-mercury-in-the-green-microalga-chlamydomonas-reinhardtii
#13
Rebecca Beauvais-Flück, Vera I Slaveykova, Claudia Cosio
Contamination by mercury (Hg) is a worldwide concern because of Hg toxicity and biomagnification in aquatic food webs. Nevertheless, bioavailability and cellular toxicity pathways of inorganic (IHg) and methyl-Hg (MeHg) remain poorly understood. We analyzed the uptake, transcriptomic, and physiological responses in the microalga Chlamydomonas reinhardtii exposed to IHg or MeHg. Bioavailability of MeHg was up to 27× higher than for IHg. Genes involved in cell processes, energy metabolism and transport were dysregulated by both Hg species...
August 14, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28808097/photoprotection-through-ultrafast-charge-recombination-in-photochemical-reaction-centres-under-oxidizing-conditions
#14
Fei Ma, David J K Swainsbury, Michael R Jones, Rienk van Grondelle
Engineering natural photosynthesis to address predicted shortfalls in food and energy supply requires a detailed understanding of its molecular basis and the intrinsic photoprotective mechanisms that operate under fluctuating environmental conditions. Long-lived triplet or singlet excited electronic states have the potential to cause photodamage, particularly in the presence of oxygen, and so a variety of mechanisms exist to prevent formation of such states or safely dissipate their energy. Here, we report a dramatic difference in spectral evolution in fully reduced and partially oxidized Rhodobacter sphaeroides reaction centres (RCs) following excitation of the monomeric bacteriochlorophyll (BChl) cofactors at 805 nm...
September 26, 2017: Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences
https://www.readbyqxmd.com/read/28807107/etiologies-and-management-of-cutaneous-flushing-nonmalignant-causes
#15
REVIEW
Azeen Sadeghian, Hailey Rouhana, Brittany Oswald-Stumpf, Erin Boh
The flushing phenomenon may represent a physiologic or a pathologic reaction. Although flushing is usually benign, it is prudent that the physician remains aware of potentially life-threatening conditions associated with cutaneous flushing. A thorough investigation should be performed if the flushing is atypical or not clearly associated with a benign underlying process. The diagnosis often relies on a pertinent history, review of systems, physical examination, and various laboratory and imaging modalities, all of which are discussed in the 2 articles in this continuing medical education series...
September 2017: Journal of the American Academy of Dermatology
https://www.readbyqxmd.com/read/28805683/analysis-and-comparison-of-the-antioxidant-component-of-portulaca-oleracea-leaves-obtained-by-different-solid-liquid-extraction-techniques
#16
Monica Gallo, Esterina Conte, Daniele Naviglio
Portulaca oleracea is a wild plant pest of orchards and gardens, but is also an edible vegetable rich in beneficial nutrients. It possesses many antioxidant properties due to the high content of vitamins, minerals, omega-3 essential fatty acids and other healthful compounds; therefore, the intake of purslane and/or its bioactive compounds could help to improve the health and function of the whole human organism. Accordingly, in this work it was analyzed and compared to the extractive capacity of the antioxidant component of purslane leaves obtained by solid-liquid extraction techniques such as: hot-maceration, maceration with ultrasound, rapid solid-liquid dynamic extraction using the Naviglio extractor, and a combination of two techniques (mix extraction)...
August 12, 2017: Antioxidants (Basel, Switzerland)
https://www.readbyqxmd.com/read/28805014/time-temperature-indicator-based-on-enzymatic-degradation-of-dye-loaded-polyhydroxybutyrate
#17
Preetam Anbukarasu, Dominic Sauvageau, Anastasia L Elias
An enzyme activated time-temperature indicator (TTI) which produces a direct colour change concomitant to variations in integrated time and temperature conditions is described. This direct colour change is realised by degrading a dye-loaded polyhydroxybutyrate (PHB) film by a depolymerase enzyme. The degradation of the PHB film by the enzyme causes the release of the dye in solution, which in turn undergoes an optical transition from clear to coloured with elapsing time. Macroscopic and microscopic optical observations confirms the uniform distribution of the dye in the PHB film...
August 14, 2017: Biotechnology Journal
https://www.readbyqxmd.com/read/28802237/umbelliferon-%C3%AE-d-glucopyranosyl-2-i-%C3%A2-1-ii-%C3%AE-dglucopyranoside-ameliorates-diethylnitrosamine-induced-precancerous-lesion-development-in-liver-via-regulation-of-inflammation-hyperproliferation-and-antioxidant-at-pre-clinical-stage
#18
Vikas Kumar, Prakash Chandra Bhatt, Mahfoozur Rahman, Fahad A Al-Abbasi, Firoz Anwar, Amita Verma
It is well documented that anomalous production of inflammatory proteins linked with most of the toxic expression and genesis of diverse chronic disease including cancer. Diethylnitrosamine (DEN) a well-known hepatotoxin and hepatocarcinogen, can induce oxidative stress and inflammatory reaction in it. Umbelliferone, secondary metabolites, is present in different plants and widely consumed by humans as medicine and food supplements. The aim of the current study was to scrutinize the chemoprotective potential of umbelliferon-α-d-glucopyranosyl-(2(I)→1(II))-α-d-glucopyranoside (UFD) against DEN-induced hepatocellular carcinoma (HCC) in experimental rats...
August 9, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28802031/quality-of-life-among-caregivers-and-growth-in-children-with-parent-reported-food-allergy
#19
Tanya Kajornrattana, Pasuree Sangsupawanich, Araya Yuenyongviwat
BACKGROUND: Some caregivers who believe their children have food allergy avoid feeding certain foods to their children without proper allergy tests. Such actions made without a proper diagnosis can negatively impact the child's health and impose an unnecessary burden on the caregivers. OBJECTIVE: This study aimed to evaluate the caregiver quality of life (QoL) and growth in children with a parent-reported food allergy. METHODS: An observational cross-sectional study was performed in 200 children younger than five years who had a parent-reported food allergy...
August 8, 2017: Asian Pacific Journal of Allergy and Immunology
https://www.readbyqxmd.com/read/28801966/ursolic-acid-a-natural-nutraceutical-agent-targets-caspase3-and-alleviates-inflammation-associated-downstream-signal-transduction
#20
Xiaoyao Ma, Yuan Zhang, Zengyong Wang, Yunbing Shen, Man Zhang, Quandeng Nie, Yuanyuan Hou, Gang Bai
SCOPE: Ursolic acid (UA) is a pentacyclicterpenoid carboxylic acid that is present in a wide variety of plant foods. There are many beneficial health effects that are attributed to the properties of UA. However, the specific cellular targets of UA and the mechanism underlying downstream signal transduction processes linked to the anti-inflammation pathway have not been thoroughly elucidated to date. METHODS AND RESULTS: Chemical biology strategies such as target fishing, click reaction synthesis of a UA probe and molecular imaging were used to identify potential target proteins of UA...
August 11, 2017: Molecular Nutrition & Food Research
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