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https://www.readbyqxmd.com/read/29228383/oral-fungal-bacterial-biofilm-models-in-vitro-a-review
#1
Marlene Chevalier, Stephane Ranque, Isabelle Prêcheur
Inclusion of fungi as commensals in oral biofilm is an important innovation in oral biology, and this work aimed to review the literature on the available biofilm and related disease in vitro models. Actually, thousands of bacterial and around one hundred of fungal phylotypes can colonize the oral cavity. Taxonomic profiling combined with functional expression analysis has revealed that Candida albicans, Streptococcus mutans and prominent periodontopathogens are not always present or numerically important in candidiasis, caries, or periodontitis lesions...
December 8, 2017: Medical Mycology: Official Publication of the International Society for Human and Animal Mycology
https://www.readbyqxmd.com/read/29224732/handheld-isothermal-amplification-and-electrochemical-detection-of-dna-in-resource-limited-settings
#2
Maria-Nefeli Tsaloglou, Alex Nemiroski, Gulden Camci-Unal, Dionysios C Christodouleas, Lara P Murray, John T Connelly, George M Whitesides
This paper demonstrates a new method for electrochemical detection of specific sequences of DNA present in trace amounts in serum or blood. This method is designed for use at the point-of-care (particularly in resource-limited settings). By combining recombinase polymerase amplification (RPA)- an isothermal alternative to the polymerase chain reaction - with an electroactive mediator, this electrochemical methodology enables accurate detection of DNA in the field using a low-cost, portable electrochemical analyzer (specifically designed for this type of analysis)...
December 7, 2017: Analytical Biochemistry
https://www.readbyqxmd.com/read/29224343/the-molecular-mechanism-of-nanodroplet-stability
#3
Evangelia Zdrali, Yixing Chen, Halil I Okur, David Mark Wilkins, Sylvie Roke
Mixtures of nano- and microscopic oil droplets in water have recently been rediscovered as miniature reaction vessels in microfluidic environments and are important constituents of many environmental systems, food, personal care and medical products. The oil nanodroplet/water interface stabilized by surfactants determines the physicochemical properties of the droplets. Surfactants are thought to stabilize nanodroplets by forming densely packed monolayers that shield the oil phase from the water. This idea has been inferred from droplet stability measurements in combination with molecular structural data obtained from extended planar interfaces...
December 11, 2017: ACS Nano
https://www.readbyqxmd.com/read/29217732/peptide-blocking-of-pd-1-pd-l1-interaction-for-cancer-immunotherapy
#4
Chunlin Li, Nengpan Zhang, Jundong Zhou, Chen Ding, Yaqing Jin, Xueyuan Cui, Kefeng Pu, Yimin Zhu
Immunotherapy has become a promising alternative therapeutic approach for cancer patients. Interruption of immune checkpoints, such as CTLA-4 and PD-1, has been verified to be a successful means for cancer therapy in clinical trials. Monoclonal antibody (mAb) targeting to PD-L1 has been approved to treat urothelial carcinoma, non-small cell lung cancer or merkel cell carcinoma by the Food and Drug Administration (FDA). However, the high cost of the antibody can limit its application. In our study, TPP-1 (targeting PD-L1 peptide), which specifically binds to PD-L1 with high affinity, was identified through bacterial surface display methods...
December 7, 2017: Cancer Immunology Research
https://www.readbyqxmd.com/read/29217480/delphinidin-protects-colon-carcinoma-cells-against-the-genotoxic-effects-of-the-mycotoxin-altertoxin-ii
#5
Georg Aichinger, Hannes Puntscher, Julia Beisl, Mary-Liis Kütt, Benedikt Warth, Doris Marko
Alternaria spp. are ubiquitous molds that are able to produce toxic secondary metabolites which may contaminate food globally. One of those is the mycotoxin altertoxin II (ATX-II), a genotoxic and mutagenic compound. In recent years, different flavonoids that may co-occur with mycotoxins in food were demonstrated to temper toxic effects of molds, mostly through their anti-oxidant properties. Thus, in this study, we assessed the influence of the berry anthocyanidin delphinidin on the toxicity of ATX-II in HT-29 colon carcinoma cells...
December 4, 2017: Toxicology Letters
https://www.readbyqxmd.com/read/29217146/the-association-between-adherence-to-a-mediterranean-style-diet-and-cognition-in-older-people-the-impact-of-medication
#6
Roy J Hardman, Denny Meyer, Greg Kennedy, Helen Macpherson, Andrew B Scholey, Andrew Pipingas
BACKGROUND: Recent reviews indicate that adherence to a Mediterranean diet may be associated with better cognitive functioning. In assessing these relationships in older individuals, previous studies have not taken into account medication usage that may support or compromise cognitive functioning. OBJECTIVE: To investigate the association between adherence to a Mediterranean style diet, cognition and medication usage in cognitively healthy older individuals. DESIGN: Data were assessed from individuals aged 60-90 years (mean = 77...
October 31, 2017: Clinical Nutrition: Official Journal of the European Society of Parenteral and Enteral Nutrition
https://www.readbyqxmd.com/read/29214141/new-trends-in-anaphylaxis
#7
REVIEW
Margitta Worm, Gunter Sturm, Jörg Kleine-Tebbe, Ewa Cichocka-Jarosz, Victoria Cardona, Ioana Maris, Sabine Dölle
This review presents the current trends in anaphylaxis management discussed at the fourth International Network for Online-Registration of Anaphylaxis (NORA) conference held in Berlin in April 2017. Current data from the anaphylaxis registry show that Hymenoptera venom, foods, and pharmaceutical drugs are still among the most frequent triggers of anaphylaxis. Rare triggers include chicory, cardamom, asparagus, and goji berries. A meta-analysis on recent trends in insect venom anaphylaxis demonstrated for the first time that, although data on the efficacy of insect venom immunotherapy is limited, the occurrence of severe reactions upon repeated sting events can be prevented and patients' quality of life improved...
2017: Allergo Journal International
https://www.readbyqxmd.com/read/29212486/emerging-concepts-and-spectrum-of-renal-injury-following-intravesical-bcg-for-non-muscle-invasive-bladder-cancer
#8
Azharuddin Mohammed, Zubair Arastu
BACKGROUND: Intravesical Bacilli Calmette-Guerin (IVBCG) therapy for non-muscle invasive bladder cancer (NMIBC) has long been in use successfully. Albeit rarely, we still face with its safety concerns more than 25 years on since its approval by US Food and Drug Agency in 1990. Local and systemic infection following intravesical BCG is widely reported as compared to immune mediated local or systemic hypersensitivity reactions involving kidneys; acute kidney injury (AKI) and other renal manifestations are well reported but not of chronic kidney disease (CKD)...
December 6, 2017: BMC Urology
https://www.readbyqxmd.com/read/29210325/foods-dietary-patterns-and-occupational-class-and-leukocyte-telomere-length-in-the-male-population
#9
Behrooz Karimi, Ramin Nabizadeh, Masud Yunesian, Parvin Mehdipour, Noushin Rastkari, Afsaneh Aghaie
Telomeres contain TTAGGG repetitive sequences and are located at the end of human chromosomes. Telomere dysfunction is associated with some age-related and chronic diseases, but its relationship with foods, dietary patterns, and occupational class in the young male population is not yet known. In this cross-sectional study, 300 healthy men, residents of Tehran, aged 25-40 years were enrolled from January to December 2016. We employed a cross-sectional study of 300 healthy people, residents of Tehran, aged 25-40 years...
November 1, 2017: American Journal of Men's Health
https://www.readbyqxmd.com/read/29208532/improvement-of-acidogenic-fermentation-for-volatile-fatty-acid-production-from-protein-rich-substrate-in-food-waste
#10
Xiaoqin Yu, Jun Yin, Dongsheng Shen, Jiali Shentu, Yuyang Long, Ting Chen
Based on our previous study, the low volatile fatty acid (VFA) production from egg white in food waste was mainly attributed to more acidogenic substrates (proteins and amino acids) consumed in the Maillard reaction and more organics converted into lactic acid. In this study, two methods were employed to improve VFA production: (1) reducing Maillard reaction with a drop in pH during hydrothermal (HT) pretreatment, and (2) inhibiting the conversion from protein to lactic acid. HT pretreatment under weakly acidic condition significantly promoted the hydrolysis and degradation of protein and the hydrolytic enzyme (protease) activity, thus increasing VFA yield by 45...
December 2, 2017: Waste Management
https://www.readbyqxmd.com/read/29205393/eaaci-guidelines-on-allergen-immunotherapy-ige-mediated-food-allergy
#11
G B Pajno, M Fernandez-Rivas, S Arasi, G Roberts, C A Akdis, M Alvaro-Lozano, K Beyer, C Bindslev-Jensen, W Burks, M Ebisawa, P Eigenmann, E Knol, K C Nadeau, L K Poulsen, R van Ree, A F Santos, G du Toit, S Dhami, U Nurmatov, Y Boloh, M Makela, L O'Mahony, N Papadopoulos, C Sackesen, I Agache, E Angier, S Halken, M Jutel, S Lau, O Pfaar, D Ryan, G Sturm, E-M Varga, R G van Wijk, A Sheikh, A Muraro
Food allergy can result in considerable morbidity, impairment of quality of life, and healthcare expenditure. There is therefore interest in novel strategies for its treatment, particularly food allergen immunotherapy (FA-AIT) through the oral (OIT), sublingual (SLIT), or epicutaneous (EPIT) routes. This Guideline, prepared by the European Academy of Allergy and Clinical Immunology (EAACI) Task Force on Allergen Immunotherapy for IgE-mediated Food Allergy, aims to provide evidence-based recommendations for active treatment of IgE-mediated food allergy with FA-AIT...
September 27, 2017: Allergy
https://www.readbyqxmd.com/read/29204266/pegloticase-in-gout-treatment-safety-issues-latest-evidence-and-clinical-considerations
#12
REVIEW
Allison Guttmann, Svetlana Krasnokutsky, Michael H Pillinger, Adey Berhanu
Gout is a common rheumatic condition, with increasing prevalence in recent decades. The mainstay of treatment for gout is oral urate-lowering therapy (ULT), typically with xanthine oxidase inhibitors (XOIs). Unfortunately, a proportion of patients have persistent gout that is refractory to ULT. Pegloticase, a recombinant pegylated uricase, has been approved by the US Food and Drug Administration for the treatment of refractory gout. However, concern has been raised regarding the risk of infusion reactions, which are now understood to be largely due to the development of antipegloticase antibodies...
December 2017: Therapeutic Advances in Drug Safety
https://www.readbyqxmd.com/read/29203956/stabilizers-of-edaravone-aqueous-solution-and-their-action-mechanisms-2-glutathione
#13
Masahiko Tanaka, Satsuki Motomiya, Akio Fujisawa, Yorihiro Yamamoto
Edaravone (3-methyl-1-phenyl-2-pyrazolin-5-one) has garnered attention since its approval for amyotrophic lateral sclerosis in Japan (2015) and the United States (2017). Edaravone is administered intravenously, and as such, is distributed in the form of an aqueous solution. However, aqueous solutions of edaravone are very unstable because they present as edaravone anions, which become edaravone radicals when the anion donates an electron to free radicals including oxygen. In this study, glutathione (GSH) stabilized an aqueous edaravone solution during storage at 60°C for 4 weeks, and prevented the formation of potentially carcinogenic phenylhydrazine, while cysteine did not...
November 2017: Journal of Clinical Biochemistry and Nutrition
https://www.readbyqxmd.com/read/29203772/integrative-transcriptomic-analysis-reveals-key-drivers-of-acute-peanut-allergic-reactions
#14
C T Watson, A T Cohain, R S Griffin, Y Chun, A Grishin, H Hacyznska, G E Hoffman, N D Beckmann, H Shah, P Dawson, A Henning, R Wood, A W Burks, S M Jones, D Y M Leung, S Sicherer, H A Sampson, A J Sharp, E E Schadt, S Bunyavanich
Mechanisms driving acute food allergic reactions have not been fully characterized. We profile the dynamic transcriptome of acute peanut allergic reactions using serial peripheral blood samples obtained from 19 children before, during, and after randomized, double-blind, placebo-controlled oral challenges to peanut. We identify genes with changes in expression triggered by peanut, but not placebo, during acute peanut allergic reactions. Network analysis reveals that these genes comprise coexpression networks for acute-phase response and pro-inflammatory processes...
December 5, 2017: Nature Communications
https://www.readbyqxmd.com/read/29202915/novel-approaches-for-the-ms-based-detection-of-food-allergens-high-resolution-ms%C3%A2-and-beyond
#15
Jens Brockmeyer
The prevalence of allergic reactions to food is believed to be increasing in industrialized countries worldwide. One of the major tasks in risk management is, therefore, the analytical surveillance of allergen contamination in food and targeted proteomics using MS, which is of hugely growing interest due to its specificity and sensitivity and the possibility to analyze multiple allergens in parallel. Though approximately 200 different foods have been described as having the potential to elicit allergic reactions, current regional labeling requirements are focused on the 5-14 priority allergens that elicit the vast majority of severe reactions or that pose a risk as hidden allergens in food production...
December 5, 2017: Journal of AOAC International
https://www.readbyqxmd.com/read/29202910/a-targeted-lc-ms-ms-method-for-the-simultaneous-detection-and-quantitation-of-egg-milk-and-peanut-allergens-in-sugar-cookies
#16
Chelsea C Boo, Christine H Parker, Lauren S Jackson
Food allergy is a growing public health concern, with many individuals reporting allergies to multiple food sources. Compliance with food labeling regulations and prevention of inadvertent cross-contact in manufacturing requires the use of reliable methods for the detection and quantitation of allergens in processed foods. In this work, a novel liquid chromatography-tandem mass spectrometry multiple-reaction monitoring method for multiallergen detection and quantitation of egg, milk, and peanut was developed and evaluated in an allergen-incurred baked sugar cookie matrix...
December 5, 2017: Journal of AOAC International
https://www.readbyqxmd.com/read/29202906/managing-food-allergens-in-the-u-k-retail-supply-chain
#17
Michael J Walker, M Hazel Gowland, John Points
The U.K. food and grocery market is highly significant financially and dominated by 10 retailers within a regulated and extremely economically competitive environment. We summarize the approach of U.K. retailers to allergen risk assessment (RA) and risk management (RM) within the U.K. legal framework and explore public visibility of retailers' allergen policies. RA and RM of allergens appear effective in curtailing retail-triggered severe food allergy reactions. However, allergen recalls remain high, precautionary allergen labeling (PAL) remains an area of confusion, and there is no consistent Web-based provision of information for consumers who have allergies...
December 5, 2017: Journal of AOAC International
https://www.readbyqxmd.com/read/29202903/food-allergen-management-in-australia
#18
Martina Koeberl, Dean Clarke, Katrina J Allen, Fiona Fleming, Lisa Katzer, N Alice Lee, Andreas L Lopata, Maria Said, Pieter Scheelings, Neil Shepherd, Robin Sherlock, James Roberts
Food allergies are increasing globally, including numbers of allergens, the sensitization rate, and the prevalence rate. To protect food-allergic individuals in the community, food allergies need to be appropriately managed. This paper describes current Australian food allergen management practices. In Australia, the prevalence of food allergies, the anaphylaxis rate, and the fatal anaphylaxis rate are among the highest in the world. Interagency and stakeholder collaboration is facilitated and enhanced as Australia moves through past, current, and ongoing food allergen challenges...
December 5, 2017: Journal of AOAC International
https://www.readbyqxmd.com/read/29202901/european-regulations-for-labeling-requirements-for-food-allergens-and-substances-causing-intolerances-history-and-future
#19
Bert Popping, Carmen Diaz-Amigo
Food allergens and intolerances have been diagnosed by doctors for decades, but have received heightened attention in the last two decades because diagnosis and awareness have increased. Consequently, regulators in many jurisdictions have addressed this topic by introducing labeling requirements for substances causing allergies and intolerance reactions in affected individuals. Mandatory labeling of food allergens allows persons suffering from these to make informed choices. However, regulations in some geographic areas have resulted in significant problems for manufacturers as well as consumers...
December 5, 2017: Journal of AOAC International
https://www.readbyqxmd.com/read/29201066/anaphylactic-shock-following-castor-bean-contact-a-case-report
#20
Y Coattrenec, D Jaques, P Jandus, T Harr, D Spoerl
Background: The castor bean plant, Ricinus communis, is known to have allergenic and toxic properties. Castor bean allergy has been described mainly as an occupational inhalation allergy in laboratory workers, in persons working in oil processing mills or in agricultural industry. So far, only one case of anaphylactic reaction due to castor bean sensitization confirmed by specific IgE has been described in literature. Case presentation: A 30-year-old woman presented to the emergency room with severe angioedema followed by urticaria, hypotension and tachycardia...
2017: Allergy, Asthma, and Clinical Immunology
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