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https://www.readbyqxmd.com/read/29458171/toxicology-studies-of-furosine-in-vitro-in-vivo-and-exploration-of-the-related-mechanism
#1
Hui-Ying Li, Lei Xing, Jia-Qi Wang, Nan Zheng
AIM: Furosine is one of the Maillard reaction products (MRPs) and is found in a variety of heat-processed food. Yet its toxicity is still unclear. The present study was designed to assess furosine toxicity in cell models and in CD-1 mice, respectively. METHODS: In vitro, the effects of furosine on the cell viability, cell cycle and apoptosis (Hek293, HepG2, SK-N-SH and Caco2) were detected and evaluated, sensitive cell lines and proper dosage of furosine for further animal experiment were determined, and the mechanisms of toxicity were explored...
February 16, 2018: Toxicology Letters
https://www.readbyqxmd.com/read/29456814/ascorbic-acid-augments-colony-spreading-by-reducing-biofilm-formation-of-methicillin-resistant-staphylococcus-aureus
#2
Zulfiqar Ali Mirani, Muhammad Naseem Khan, Anila Siddiqui, Fouzia Khan, Mubashir Aziz, Shagufta Naz, Ayaz Ahmed, Seema Ismat Khan
Objectives: Staphylococcus aureus is a Gram-positive pathogen, well known for its resistance and versatile lifestyle. Under unfavourable conditions, it adapts biofilm mode of growth. For staphylococcal biofilm formation, production of extracellular polymeric substances (EPS) is a pre-requisite, which is regulated by ica operon-encoded enzymes. This study was designed to know the impact of ascorbic acid on biofilm formation and colony spreading processes of S. aureus and MRSA. Materials and Methods: The isolates of methicillin-resistant S...
February 2018: Iranian Journal of Basic Medical Sciences
https://www.readbyqxmd.com/read/29455734/bamboo-salt-suppresses-skin-inflammation-in-mice-with-2-4-dinitrofluorobenzene-induced-atopic-dermatitis
#3
Myoung-Schook Yoou, Sun-Young Nam, Kyoung Wan Yoon, Hyun-Ja Jeong, Hyung-Min Kim
Bamboo salt (BS) is a traditional Korean food, and has been reported to have anti-cancer, anti-inflammatory, and anti-metastatic effects. However, the anti-atopic dermatitis (AD) activity of BS has not been described yet. In the present study, we examined the preventive effect of BS on AD. The effect of oral administration of BS was tested in a 2, 4-dinitrofluorobenzene (DNFB)-induced AD animal model, by histological analysis, enzyme-linked immunosorbent assay, reverse transcription-polymerase chain reaction, caspase-1 assay, and Western blotting analysis...
February 2018: Chinese Journal of Natural Medicines
https://www.readbyqxmd.com/read/29455358/the-use-of-biomarkers-to-predict-aero-allergen-and-food-immunotherapy-responses
#4
REVIEW
Sayantani B Sindher, Andrew Long, Swati Acharya, Vanitha Sampath, Kari C Nadeau
The incidence of allergic conditions has continued to rise over the past several decades, with a growing body of research dedicated toward the treatment of such conditions. By driving a complex range of changes in the underlying immune response, immunotherapy is the only therapy that modulates the immune system with long-term effects and is presently utilized for the treatment of several atopic conditions. Recent efforts have focused on identifying biomarkers associated with these changes that may be of use in predicting patients with the highest likelihood of positive clinical outcomes during allergen immunotherapy (AIT), providing guidance regarding AIT discontinuation, and predicting symptomatic relapse and the need for booster AIT after therapy...
February 17, 2018: Clinical Reviews in Allergy & Immunology
https://www.readbyqxmd.com/read/29455192/cloning-and-expression-analysis-of-cdnas-encoding-adp-glucose-pyrophosphorylase-large-and-small-subunits-from-hulless-barley-hordeum-vulgare-l-var-nudum
#5
Dongmei Li, Zhimin Yang, Xinchun Liu, Zhen Song, Zongyun Feng, Yang He
As an important plateau cereal crop, hulless barley is the principal food for the Tibetan people in China. ADP-glucose pyrophosphorylase (AGPase) is considered as the key enzyme for starch biosynthesis in plants. In this study, cDNAs encoding the small subunit (SSU I) and large subunit (LSU I) of AGPase were isolated from hulless barley. The results showed that SSU I and LSU I were 1438 and 1786 bp in length with a complete open reading frame (ORF) of 1419 and 1572 bp. The ORF-encoded polypeptides of 472 and 523 amino acids were having calculated molecular masses of 52...
February 19, 2018: Zeitschrift Für Naturforschung. C, A Journal of Biosciences
https://www.readbyqxmd.com/read/29453261/molecular-and-functional-study-of-a-branching-sucrase-like-glucansucrase-reveals-an-evolutionary-intermediate-between-two-subfamilies-of-the-gh70-enzymes
#6
Minghui Yan, Bing-Hua Wang, Xiaofen Xu, Peng Chang, Feng Hang, Zhengjun Wu, Chunping You, Zhenmin Liu
Family GH70 glucansucrases (GSs) catalyze the synthesis of α-glucans from sucrose, a reaction widely seen in lactic acid bacteria (LAB). These enzymes have been implicated in many aspects of the microbial life. Products of GSs have great commercial value as food supplements and medical materials, therefore these enzymes have attracted much attention from both fields of science and industry. However, certain issues concerning the origin and evolution of GS are still to be addressed, although an increasing number of GH70 enzymes have been characterized...
February 16, 2018: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/29451805/loss-and-formation-of-malodorous-volatile-sulfhydryl-compounds-during-wine-storage
#7
Gal Y Kreitman, Ryan J Elias, David W Jeffery, Gavin L Sacks
Volatile sulfur compounds (VSCs), particularly low molecular weight sulfhydryls like hydrogen sulfide (H 2 S) and methanethiol (MeSH), are often observed in wines with sulfurous off-aromas. Recent work has shown both H 2 S and MeSH can increase up to a few µM (> 40 µg/L) during anoxic storage, but the identity of the latent sources of these sulfhydryls is still disputed. This review critically evaluates the latent precursors and pathways likely to be responsible for the loss and formation of these sulfhydryls during wine storage based on the existing enology literature as well as studies from food chemistry, geochemistry, biochemistry, and synthetic chemistry...
February 16, 2018: Critical Reviews in Food Science and Nutrition
https://www.readbyqxmd.com/read/29449885/deep-eutectic-solvents-pretreatment-of-agro-industrial-food-waste
#8
Alessandra Procentese, Francesca Raganati, Giuseppe Olivieri, Maria Elena Russo, Lars Rehmann, Antonio Marzocchella
Background: Waste biomass from agro-food industries are a reliable and readily exploitable resource. From the circular economy point of view, direct residues from these industries exploited for production of fuel/chemicals is a winning issue, because it reduces the environmental/cost impact and improves the eco-sustainability of productions. Results: The present paper reports recent results of deep eutectic solvent (DES) pretreatment on a selected group of the agro-industrial food wastes (AFWs) produced in Europe...
2018: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/29448943/cloning-and-expression-of-the-sucrose-phosphorylase-gene-in-bacillus-subtilis-and-synthesis-of-kojibiose-using-the-recombinant-enzyme
#9
Miaomiao Wang, Jing Wu, Dan Wu
BACKGROUND: Kojibiose as a prebiotic and inhibitor of α-glucosidase exhibits potential for a wide range of applications in the food and medicine fields; however, large-scale separation and extraction of kojibiose from nature is difficult. Sucrose phosphorylase (SPase) can be used for the production of kojibiose, and currently, SPase is only heterologously expressed in E. coli, making it unsuitable for use in the food industry. However, Bacillus subtilis is generally considered to be a safe organism potentially useful for SPase expression...
February 15, 2018: Microbial Cell Factories
https://www.readbyqxmd.com/read/29448094/the-prevalence-and-antimicrobial-resistance-of-listeria-spp-in-raw-milk-and-traditional-dairy-products-delivered-in-yazd-central-iran-2016
#10
Fateme Akrami-Mohajeri, Zahra Derakhshan, Margherita Ferrantee, Negar Hamidiyan, Meysam Soleymani, Gea Oliveri Conti
Listeria monocytogenes is an important foodborne pathogen which its data in Iranian dairy products is limited. A total of 545 samples of traditional dairy products (raw milk, traditional cheese, traditional butter, traditional curd, and traditional ice cream) were collected from traditional dairy shops located in Yazd, Iran. L. monocytogenes was isolated, and positive samples were confirmed by polymerase chain reaction. Antibiotic sensitivity test was conducted to determine the antibiotic resistance. Listeria species were isolated from 11...
February 12, 2018: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/29447579/double-gene-targeting-pcr-assay-for-the-detection-of-crocodylus-porosus-in-commercial-products
#11
Nina Naquiah, Md Eaqub Ali, M A Motalib Hossain, Sharmin Sultana, Mohammad Nasir Uddin Ahamad
The demand for crocodile meat is quickly growing because of its exotic and organoleptic appeal and also low content of cholesterol and lipids. Moreover, crocodile oil and blood have been used in alternative medicines for treating asthma and several other ailments since ancient times. Furthermore, crocodile hides have great demand in leather industries. All of these have collectively contributed to the extensive hunting, illegal trading and consequent decline of crocodiles in most parts of the world. To keep space with the growing demands, some crocodile species such as Crocodylus porosus have been raised in farms and its commercial trades have been legalized...
February 15, 2018: Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment
https://www.readbyqxmd.com/read/29446074/a-hitchhiker-s-guide-to-humanized-mice-new-pathways-to-studying-viral-infections
#12
REVIEW
J K Skelton, A M Ortega-Prieto, M Dorner
Humanized mice are increasingly appreciated as an incredibly powerful platform for infectious disease research. The often very narrow species tropism of many viral infections, coupled with the sometimes-misleading results from preclinical studies in animal models (e.g. fatal immune reactions in the PEARL study 1 ) further emphasize the need for more predictive model systems based on human cells rather than surrogates. Humanized mice represent such a model and have been greatly enhanced in regards to their immune system reconstitution as well as immune functionality in the past years, resulting in their recommendation as pre-clinical model by the US Food and Drug Administration...
February 15, 2018: Immunology
https://www.readbyqxmd.com/read/29445832/analytical-challenges-in-sports-drug-testing
#13
Mario Thevis, Oliver Krug, Hans Geyer, Katja Walpurgis, Norbert Baume, Andreas Thomas
Analytical chemistry represents a central aspect of doping controls. Routine sports drug testing approaches are primarily designed to address the question whether a prohibited substance is present in a doping control sample and whether prohibited methods (for example, blood transfusion or sample manipulation) have been conducted by an athlete. As some athletes have availed themselves of the substantial breadth of research and development in the pharmaceutical arena, proactive and preventive measures are required such as the early implementation of new drug candidates and corresponding metabolites into routine doping control assays, even though these drug candidates are to date not approved for human use...
February 14, 2018: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/29444823/introduction-to-the-thematic-minireview-series-green-biological-chemistry
#14
Joseph M Jez
Plants and their green cousins cyanobacteria and algae use sunlight to drive the chemistry that lets them grow, survive, and perform an amazing range of biochemical reactions. The ability of these organisms to use a freely available energy source makes them attractive for use as sustainable and renewable platforms for more than just food production. They are also a source of metabolic tools for engineering microbes for "green" chemistry. This thematic collection of mini-reviews discusses how green organisms capture light and protect their photosynthetic machinery from too much light, new structural snapshots of the clock complex that orchestrates signaling during the light/dark cycle, challenges for improving stress responses in crops, harnessing cyanobacteria for as sustainable factories, and efforts to engineer microbes for "green" biopolymer production...
February 14, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29442085/an-insight-into-diagnosis-of-a-hidden-entity-impacted-food-material
#15
Humeera M Mulla, Nupura Vibhute, Rajendra Baad, N D Shashikiran, Mustaq Parker, R C Parmod, Uzma Belgaumi, Vidya Kadashetti, Sushma Bommanawar
Introduction: Foreign bodies and tissue reactions to foreign materials are commonly encountered in the oral cavity. Exogenous materials causing foreign body reactions may be metallic (amalgam) or nonmetallic (suture materials, vegetable matter). Implantation of food particles in the oral tissues has been known to cause reactive lesions such as oral pulse granuloma. Implantation could be through extraction sockets, deep periodontal pockets, associated with tumor growth, interdental areas of teeth, unfilled root canals, and grossly decayed teeth...
January 2018: Indian Journal of Dental Research: Official Publication of Indian Society for Dental Research
https://www.readbyqxmd.com/read/29440726/a-chemoenzymatic-process-for-amide-bond-formation-by-an-adenylating-enzyme-mediated-mechanism
#16
Ryotaro Hara, Kengo Hirai, Shin Suzuki, Kuniki Kino
Amide bond formation serves as a fundamental reaction in chemistry, and is practically useful for the synthesis of peptides, food additives, and polymers. However, current methods for amide bond formation essentially generate wastes and suffer from poor atom economy under harsh conditions. To solve these issues, we demonstrated an alternative synthesis method for diverse tryptophyl-N-alkylamides by the combination of the first adenylation domain of tyrocidine synthetase 1 with primary or secondary amines as nucleophiles...
February 13, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29439931/does-low-protein-diet-influence-the-uremic-toxin-serum-levels-from-the-gut-microbiota-in-nondialysis-chronic-kidney-disease-patients
#17
Ana Paula Black, Juliana S Anjos, Ludmila Cardozo, Flávia L Carmo, Carla J Dolenga, Lia S Nakao, Dennis de Carvalho Ferreira, Alexandre Rosado, José Carlos Carraro Eduardo, Denise Mafra
OBJECTIVES: To evaluate the effects of low-protein diet (LPD) on uremic toxins and the gut microbiota profile in nondialysis chronic kidney disease (CKD) patients. DESIGN AND METHODS: Longitudinal study with 30 nondialysis CKD patients (stage 3-4) undergoing LPD for 6 months. Adherence to the diet was evaluated based on the calculation of protein equivalent of nitrogen appearance from the 24-hour urine analysis. Good adherence to LPD was considered when protein intake was from 90% to 110% of the prescribed amount (0...
February 10, 2018: Journal of Renal Nutrition
https://www.readbyqxmd.com/read/29438186/peanut-allergy-reduction-in-high-risk-pediatric-patients
#18
Jaime Hopper, Courtney Hopp, Jessica Durbin
The prevalence of food allergies has doubled in the past 10 years. Peanut allergies are a significant public health issue and are the primary reason for food-related anaphylactic reactions that result in death. Evidence supports that early introduction of the peanut protein (or in combination with immunotherapy) to the highly allergic may safely desensitize patients, which could lead to less adverse allergic reactions and alter allergy management overall.
March 12, 2018: Nurse Practitioner
https://www.readbyqxmd.com/read/29437768/fda-approval-ribociclib-for-the-treatment-of-postmenopausal-women-with-hormone-receptor-positive-her2-negative-advanced-or-metastatic-breast-cancer
#19
Anand Shah, Erik Bloomquist, Shenghui Tang, Wentao Fu, Youwei Bi, Qi Liu, Jingyu Yu, Ping Zhao, Todd R Palmby, Kirsten B Goldberg, C J George Chang, Paresma Patel, Elleni Alebachew, Amy Tilley, William F Pierce, Amna Ibrahim, Gideon M Blumenthal, Rajeshwari Sridhara, Julia A Beaver, Richard Pazdur
On March 13, 2017, the U.S. Food and Drug Administration approved ribociclib (KISQALI, Novartis Pharmaceuticals Corp.), a cyclin-dependent kinase 4/6 inhibitor, in combination with an aromatase inhibitor as initial endocrine-based therapy for the treatment of postmenopausal women with hormone receptor (HR)-positive, human epidermal growth factor receptor 2 (HER2)-negative advanced or metastatic breast cancer. The approval was based on a randomized, double-blind, placebo-controlled, international clinical trial (MONALEESA-2)...
February 7, 2018: Clinical Cancer Research: An Official Journal of the American Association for Cancer Research
https://www.readbyqxmd.com/read/29437236/a-novel-modified-lipid-enzymatic-esterification-of-2-monoacylglycerol-with-n-acetyl-l-leucine
#20
Hasene Keskin Çavdar, Derya Koçak Yanık, Fahrettin Göğüş, Sibel Fadıloğlu
The objective of this research was to synthesize and characterize a novel modified lipid which is composed of mainly oleic acid at sn-2 position and a hydrophobic essential amino acid derivative (N-acetyl-l-leucine) at sn-1(3) or sn-1, 3 positions. LC-MS-MS analysis indicated that the target modified lipid structure was obtained at the end of the developed reaction. Response surface methodology was applied to enhance a reliable empirical model for prediction and to optimize reaction conditions: reaction time (8 to 24 hr), reaction temperature (40 to 55 °C), and enzyme load (15 to 30 wt%)...
February 13, 2018: Journal of Food Science
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