keyword
https://read.qxmd.com/read/38103028/fungal-microbiota-composition-in-inflammatory-bowel-disease-patients-characterization-in-different-phenotypes-and-correlation-with-clinical-activity-and-disease-course
#21
JOURNAL ARTICLE
Ignacio Catalán-Serra, Silje Thorsvik, Vidar Beisvag, Torunn Bruland, David Underhill, Arne Kristian Sandvik, Atle van Beelen Granlund
BACKGROUND: There is growing evidence of the role of the mycobiome in inflammatory bowel disease (IBD). Variations within phenotypes and activity and with prognosis have been poorly studied. METHODS: A total of 111 individuals were prospectively enrolled: 89 IBD patients (52 ulcerative colitis and 37 Crohn's disease [CD]) and 22 healthy individuals. Disease characteristics were collected and a fecal calprotectin >100 μg/mg was considered indicative of activity...
December 16, 2023: Inflammatory Bowel Diseases
https://read.qxmd.com/read/38072057/the-glycerophosphocholine-acyltransferase-gpc1-contributes-to-phosphatidylcholine-biosynthesis-long-term-viability-and-embedded-hyphal-growth-in-candida-albicans
#22
JOURNAL ARTICLE
William R King, Justin Singer, Mitchell Warman, Duncan Wilson, Bernard Hube, Ida Lager, Jana Patton-Vogt
Candida albicans is a commensal fungus, opportunistic pathogen, and the most common cause of fungal infection in humans. The biosynthesis of phosphatidylcholine (PC), a major eukaryotic glycerophospholipid, occurs through two primary pathways. In Saccharomyces cerevisiae and some plants, a third PC synthesis pathway, the PC deacylation/reacylation pathway (PC-DRP), has been characterized. PC-DRP begins with the acylation of the lipid turnover product, glycerophosphocholine (GPC), by the GPC acyltransferase, Gpc1, to form Lyso-PC...
December 8, 2023: Journal of Biological Chemistry
https://read.qxmd.com/read/38054042/farnesol-as-an-antifungal-agent-comparisons-among-mtl-a-and-mtl%C3%AE-haploid-and-diploid-candida-albicans-and-saccharomyces-cerevisiae
#23
JOURNAL ARTICLE
Cory H T Boone, Kory A Parker, Daniel J Gutzmann, Audrey L Atkin, Kenneth W Nickerson
Aims: Farnesol was identified 20 years ago in a search for Candida albicans quorum sensing molecules (QSM), but there is still uncertainty regarding many aspects of its mode of action including whether it employs farnesol transport mechanisms other than diffusion. Based on the structural similarity between farnesol and the farnesylated portion of the MTL a pheromone, we explored the effects of ploidy and mating type locus ( MTL ) on the antifungal activity of exogenous farnesol. Methods and results: We approached this question by examining five MTL a and five MTLα haploid strains with regard to their farnesol sensitivity in comparison to six heterozygous MTL a / α diploids...
2023: Frontiers in Physiology
https://read.qxmd.com/read/38040100/from-asca-breakthrough-in-crohn-s-disease-and-candida-albicans-research-to-thirty-years-of-investigations-about-their-meaning-in-human-health
#24
REVIEW
Boualem Sendid, Marjorie Cornu, Camille Cordier, Julie Bouckaert, Jean Frederic Colombel, Daniel Poulain
Anti-Saccharomyces cerevisiae antibodies (ASCA) are human antibodies that can be detected using an enzyme-linked immunosorbent assay involving a mannose polymer (mannan) extracted from the cell wall of the yeast S. cerevisiae. The ASCA test was developed in 1993 with the aim of differentiating the serological response in two forms of inflammatory bowel disease (IBD), Crohn's disease and ulcerative colitis. The test, which is based on the detection of anti-oligomannosidic antibodies, has been extensively performed worldwide and there have been hundreds of publications on ASCA...
November 30, 2023: Autoimmunity Reviews
https://read.qxmd.com/read/37998902/the-early-endocytosis-gene-pal1-contributes-to-stress-tolerance-and-hyphal-formation-in-candida-albicans
#25
JOURNAL ARTICLE
Miranda Yu, Dakota Ma, Susan Eszterhas, Christiane Rollenhagen, Samuel A Lee
The endocytic and secretory pathways of the fungal pathogen Candida albicans are fundamental to various key cellular processes such as cell growth, cell wall integrity, protein secretion, hyphal formation, and pathogenesis. Our previous studies focused on several candidate genes involved in early endocytosis, including ENT2 and END3 , that play crucial roles in such processes. However, much remains to be discovered about other endocytosis-related genes and their contributions toward Candida albicans secretion and virulence...
November 10, 2023: Journal of Fungi (Basel, Switzerland)
https://read.qxmd.com/read/37969749/spot-assay-and-colony-forming-unit-cfu-analyses-based-sensitivity-test-for-candida-albicans-and-saccharomyces-cerevisiae
#26
JOURNAL ARTICLE
Satya Ranjan Sahu, Bhabasha Gyanadeep Utkalaja, Shraddheya Kumar Patel, Narottam Acharya
Cellular sensitivity is an approach to inhibit the growth of certain cells in response to any non-permissible conditions, as the presence of a cytotoxic agent or due to changes in growth parameters such as temperature, salt, or media components. Sensitivity tests are easy and informative assays to get insight into essential gene functions in various cellular processes. For example, cells having any functionally defective genes involved in DNA replication exhibit sensitivity to non-permissive temperatures and to chemical agents that block DNA replication fork movement...
November 5, 2023: Bio-protocol
https://read.qxmd.com/read/37900111/cell-cycle-analysis-of-candida-albicans-by-flow-cytometry
#27
JOURNAL ARTICLE
Shraddheya Kumar Patel, Satya Ranjan Sahu, Narottam Acharya
The cell cycle is a vital process of cell division that is required to sustain life. Since faithful cell division is critical for the proper growth and development of an organism, the study of the cell cycle becomes a fundamental research objective. Saccharomyces cerevisiae has been an excellent unicellular system for unraveling the secrets of cell division, and the process of synchronization in budding yeast has been standardized. Cell synchronization is a crucial step of cell cycle analysis, where cells in a culture at different stages of the cell cycle are arrested to the same phase and, upon release, they progress synchronously...
October 20, 2023: Bio-protocol
https://read.qxmd.com/read/37888291/evaluation-of-antifungal-selective-toxicity-using-candida-glabrata-erg25-and-human-sc4mol-knock-in-strains
#28
JOURNAL ARTICLE
Keiko Nakano, Michiyo Okamoto, Azusa Takahashi-Nakaguchi, Kaname Sasamoto, Masashi Yamaguchi, Hiroji Chibana
With only four classes of antifungal drugs available for the treatment of invasive systemic fungal infections, the number of resistant fungi is increasing, highlighting the urgent need for novel antifungal drugs. Ergosterol, an essential component of cell membranes, and its synthetic pathway have been targeted for antifungal drug development. Sterol-C4-methyl monooxygenase (Erg25p), which is a greater essential target than that of existing drugs, represents a promising drug target. However, the development of antifungal drugs must consider potential side effects, emphasizing the importance of evaluating their selective toxicity against fungi...
October 20, 2023: Journal of Fungi (Basel, Switzerland)
https://read.qxmd.com/read/37877405/-expression-of-bmspi38-tandem-multimers-in-escherichia-coli-and-its-antifungal-activity
#29
JOURNAL ARTICLE
Youshan Li, Yuan Wang, Rui Zhu, Xi Yang, Meng Wei, Zhaofeng Zhang, Changqing Chen
The aim of this study was to prepare tandem multimeric proteins of BmSPI38, a silkworm protease inhibitor, with better structural homogeneity, higher activity and stronger antifungal ability by protein engineering. The tandem multimeric proteins of BmSPI38 were prepared by prokaryotic expression technology. The effects of tandem multimerization on the structural homogeneity, inhibitory activity and antifungal ability of BmSPI38 were explored by in-gel activity staining of protease inhibitor, protease inhibition assays and fungal growth inhibition experiments...
October 25, 2023: Sheng Wu Gong Cheng Xue Bao, Chinese Journal of Biotechnology
https://read.qxmd.com/read/37849458/peptide-variant-detection-by-a-living-yeast-biosensor-via-an-epitope-selective-protease
#30
JOURNAL ARTICLE
Tea Crnković, Benjamin J Bokor, Mead E Lockwood, Virginia W Cornish
We previously demonstrated that we could hijack the fungal pheromone signaling pathway to provide a living yeast biosensor where peptide biomarkers were recognized by G-protein-coupled receptors and engineered to transcribe a readout. Here, we demonstrated that the protease could be reintroduced to the biosensor to provide a simple mechanism for distinguishing single-amino-acid changes in peptide ligands that, otherwise, would likely be difficult to detect using binding-based assays. We characterized the dose-response curves for five fungal pheromone G-protein-coupled receptors, peptides, and proteases -Saccharomyces cerevisiae , Candida albicans , Schizosaccharomyces pombe , Schizosaccharomyces octosporus , and Schizosaccharomyces japonicus ...
2023: Biodes Res
https://read.qxmd.com/read/37836118/phytochemical-composition-and-pharmacological-activities-of-three-essential-oils-collected-from-eastern-morocco-origanum-compactum-salvia-officinalis-and-syzygium-aromaticum-a-comparative-study
#31
JOURNAL ARTICLE
El Hassania Loukili, Safae Ouahabi, Amine Elbouzidi, Mohamed Taibi, Meryem Idrissi Yahyaoui, Abdeslam Asehraou, Abdellah Azougay, Asmaa Saleh, Omkulthom Al Kamaly, Mohammad Khalid Parvez, Bouchra El Guerrouj, Rachid Touzani, Mohammed Ramdani
Throughout history, essential oils have been employed for their pleasing scents and potential therapeutic benefits. These oils have shown promise in various areas, including aromatherapy, personal care products, natural remedies, and even as alternatives to traditional cleaning agents or pest control solutions. The study aimed to explore the chemical makeup, antioxidant, and antibacterial properties of Origanum compactum Benth., Salvia officinalis L., and Syzygium aromaticum (L.) Merr. et Perry. Initially, the composition of the three essential oils, O...
September 25, 2023: Plants (Basel, Switzerland)
https://read.qxmd.com/read/37804207/regulatory-and-pathogenic-mechanisms-in-response-to-iron-deficiency-and-excess-in-fungi
#32
REVIEW
Jordi Pijuan, David F Moreno, Galal Yahya, Mihaela Moisa, Ihtisham Ul Haq, Katarzyna Krukiewicz, Rasha Mosbah, Kamel Metwally, Simona Cavalu
Iron is an essential element for all eukaryote organisms because of its redox properties, which are important for many biological processes such as DNA synthesis, mitochondrial respiration, oxygen transport, lipid, and carbon metabolism. For this reason, living organisms have developed different strategies and mechanisms to optimally regulate iron acquisition, transport, storage, and uptake in different environmental responses. Moreover, iron plays an essential role during microbial infections. Saccharomyces cerevisiae has been of key importance for decrypting iron homeostasis and regulation mechanisms in eukaryotes...
October 7, 2023: Microbial Biotechnology
https://read.qxmd.com/read/37798667/antifungal-bio-coating-of-endotracheal-tube-built-by-overexpressing-the-mcp1-gene-of-saccharomyces-boulardii-and-employing-hydrogel-as-a-house-to-antagonize-candida-albicans
#33
JOURNAL ARTICLE
Yunyun Wei, Jianfeng Qiu, Ziqiang Han, Xuanyi Wang, Hui Zhang, Xinya Hou, Xiangwei Lv, Xiaolong Mao
BACKGROUND: For some ICU patients, an artificial airway must be established with an endotracheal tube, but Candida albicans can easily adhere to the tube surface and form a biofilm, leading to potentially life threatening fungal infections. Therefore, it is urgent to prevent and reduce C. albicans infections introduced by the endotracheal tube. However, there are few antifungal drugs effective against C. albicans, and each of these drugs may have adverse effects on human cells. Saccharomyces boulardii is regarded as an alternative strategy to inhibit the adhesion of C...
October 5, 2023: Biomaterials Research
https://read.qxmd.com/read/37793775/functional-plasticity-in-chromosome-microtubule-coupling-on-the-evolutionary-time-scale
#34
JOURNAL ARTICLE
Sundar Ram Sankaranarayanan, Satya Dev Polisetty, Kuladeep Das, Arti Dumbrepatil, Bethan Medina-Pritchard, Martin Singleton, A Arockia Jeyaprakash, Kaustuv Sanyal
The Dam1 complex is essential for mitotic progression across evolutionarily divergent fungi. Upon analyzing amino acid (aa) sequences of Dad2, a Dam1 complex subunit, we identified a conserved 10-aa-long Dad2 signature sequence (DSS). An arginine residue (R126) in the DSS is essential for viability in Saccharomyces cerevisiae that possesses point centromeres. The corresponding arginine residues are functionally important but not essential for viability in Candida albicans and Cryptococcus neoformans ; both carry several kilobases long regional centromeres...
December 2023: Life Science Alliance
https://read.qxmd.com/read/37791798/the-adr1-transcription-factor-directs-regulation-of-the-ergosterol-pathway-and-azole-resistance-in-candida-albicans
#35
JOURNAL ARTICLE
Manjari Shrivastava, Gaëlle S Kouyoumdjian, Eftyhios Kirbizakis, Daniel Ruiz, Manon Henry, Antony T Vincent, Adnane Sellam, Malcolm Whiteway
Transcription factors (TFs) play key roles in cellular regulation and are critical in the control of drug resistance in the fungal pathogen Candida albicans . We found that activation of the transcription factor C4_02500C_A (Adr1) conferred significant resistance against fluconazole. In Saccharomyces cerevisiae , Adr1 is a carbon-source-responsive zinc-finger transcription factor required for transcription of the glucose-repressed gene ADH1 and of genes required for ethanol, glycerol, and fatty acid utilization...
October 4, 2023: MBio
https://read.qxmd.com/read/37781717/heme-sensing-and-trafficking-in-fungi
#36
JOURNAL ARTICLE
Peng Xue, Eddy Sánchez-León, Djihane Damoo, Guanggan Hu, Won Hee Jung, James W Kronstad
Fungal pathogens cause life-threatening diseases in humans, and the increasing prevalence of these diseases emphasizes the need for new targets for therapeutic intervention. Nutrient acquisition during infection is a promising target, and recent studies highlight the contributions of endomembrane trafficking, mitochondria, and vacuoles in the sensing and acquisition of heme by fungi. These studies have been facilitated by genetically encoded biosensors and other tools to quantitate heme in subcellular compartments and to investigate the dynamics of trafficking in living cells...
March 2023: Fungal Biology Reviews
https://read.qxmd.com/read/37776725/oral-fungal-profiling-and-risk-of-nasopharyngeal-carcinoma-a-population-based-case-control-study
#37
JOURNAL ARTICLE
Yufeng Chen, Wanxin Li, Ellen T Chang, Justine W Debelius, Lokeshwaran Manoharan, Yuming Zheng, Yancheng Li, Guangwu Huang, Hans-Olov Adami, Rob Knight, Yonglin Cai, Zhe Zhang, Weimin Ye
BACKGROUND: Dysbiosis of the oral mycobiome has been linked to some diseases, including cancers. However, the role of oral fungal communities in nasopharyngeal carcinoma (NPC) carcinogenesis has not previously been investigated. METHODS: We characterized the oral salivary fungal mycobiome in 476 untreated incident NPC patients and 537 population-based controls using fungal internal transcribed spacer (ITS)-2 sequencing. The relationship between oral fungal mycobiome and the risk of NPC was assessed through bioinformatic and biostatistical analyses...
September 28, 2023: EBioMedicine
https://read.qxmd.com/read/37761150/antimicrobial-activity-of-frankincense-boswellia-sacra-oil-and-smoke-against-pathogenic-and-airborne-microbes
#38
JOURNAL ARTICLE
Zahra S Al-Kharousi, Ann S Mothershaw, Basil Nzeako
As they continuously evolve, plants will remain a renewable source for antimicrobial compounds. Omani frankincense is produced by B. sacra trees and is graded into Hojari, Nejdi, Shazri or Sha'bi. Air can be a source for pathogenic or food spoilage microbes; thus, inactivating airborne microbes is necessary in environments such as food and animal production areas. This study investigated the antimicrobial activity and the chemistry of steam-distilled oils of Hojari and Sha'bi grades. It also analyzed the antimicrobial activity of frankincense smoke and the size of its solid particles...
September 15, 2023: Foods (Basel, Switzerland)
https://read.qxmd.com/read/37755709/study-of-chemical-constituents-antioxidants-and-antimicrobial-activities-of-tamarindus-indica-l-seed
#39
JOURNAL ARTICLE
Edinéia Bonin, Vicente Diaz Avila, Venício Macedo Carvalho, Melina Aparecida Plastina Cardoso, Aylle Medeiros Matos, Anderson Valdiney Gomes Ramos, Márcia Regina Pereira Cabral, Debora Cristina Baldoqui, Maria Helena Sarragiotto, Benício Alves de Abreu Filho, Ivanor Nunes do Prado
The fruits of Tamarindus indica L. are consumed worldwide, with various parts of the plant being used for medicinal purposes. The residues (pericarp and seeds) generated during cellulose processing are of significant value as they contain bioactive compounds with diverse biological activities. The objective of this study was to evaluate the chemical constituents of the ethyl acetate fraction as possible substitutes for synthetic compounds with biological properties using ultra-high performance liquid chromatography coupled to high-resolution mass spectrometry (UHPLC-HRMS/MS) analysis and the evaluation of the antioxidant activity (ferric reducing antioxidant power [FRAP], 2,2'-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid [ABTS], and 1-diphenyl-2-picrylhydrazyl [DPPH]), total phenolic compounds (TPC), and antimicrobial activity of the hydroalcoholic extract and tamarind seed fractions were also performed...
September 27, 2023: Journal of Food Science
https://read.qxmd.com/read/37755056/the-use-of-thioflavin-t-for-the-estimation-and-measurement-of-the-plasma-membrane-electric-potential-difference-in-different-yeast-strains
#40
JOURNAL ARTICLE
Antonio Peña, Norma Silvia Sánchez, Francisco Padilla-Garfias, Yazmín Ramiro-Cortés, Minerva Araiza-Villanueva, Martha Calahorra
The use of the cationic, dye thioflavin T (ThT), to estimate the electric plasma membrane potential difference (PMP) via the fluorescence changes and to obtain its actual values from the accumulation of the dye, considering important correction factors by its binding to the internal components of the cell, was described previously for baker's yeast. However, it was considered important to explore whether the method developed could be applied to other yeast strains. Alternative ways to estimate the PMP by using flow cytometry and a multi-well plate reader are also presented here...
September 20, 2023: Journal of Fungi (Basel, Switzerland)
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