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https://www.readbyqxmd.com/read/28580409/data-from-proteome-analysis-of-lasiodiplodia-theobromae-botryosphaeriaceae
#1
Carla C Uranga, Majid Ghassemian, Rufina Hernández-Martínez
Trunk disease fungi are a global problem affecting many economically important fruiting trees. The Botryosphaeriaceae are a family of trunk disease fungi that require detailed biochemical characterization in order to gain insight into their pathogenicity. The application of a modified Folch extraction to protein extraction from the Botryosphaeriaceae Lasiodiplodia theobromae generated an unprecedented data set of protein identifications from fragmentation analysis and de novo peptide sequencing of its proteome...
August 2017: Data in Brief
https://www.readbyqxmd.com/read/28577425/denaturing-gradient-gel-electrophoresis-profiles-of-bacteria-from-the-saliva-of-twenty-four-different-individuals-form-clusters-that-showed-no-relationship-to-the-yeasts-present
#2
Manjula M Weerasekera, Chris H Sissons, Lisa Wong, Sally A Anderson, Ann R Holmes, Richard D Cannon
OBJECTIVES: The aim was to investigate the relationship between groups of bacteria identified by cluster analysis of the DGGE fingerprints and the amounts and diversity of yeast present. METHODS: Bacterial and yeast populations in saliva samples from 24 adults were analysed using denaturing gradient gel electrophoresis (DGGE) of the bacteria present and by yeast culture. RESULTS: Eubacterial DGGE banding patterns showed considerable variation between individuals...
May 22, 2017: Archives of Oral Biology
https://www.readbyqxmd.com/read/28566496/phosphate-is-the-third-nutrient-monitored-by-tor-in-candida-albicans-and-provides-a-target-for-fungal-specific-indirect-tor-inhibition
#3
Ning-Ning Liu, Peter R Flanagan, Jumei Zeng, Niketa M Jani, Maria E Cardenas, Gary P Moran, Julia R Köhler
The Target of Rapamycin (TOR) pathway regulates morphogenesis and responses to host cells in the fungal pathogen Candida albicans Eukaryotic Target of Rapamycin complex 1 (TORC1) induces growth and proliferation in response to nitrogen and carbon source availability. Our unbiased genetic approach seeking unknown components of TORC1 signaling in C. albicans revealed that the phosphate transporter Pho84 is required for normal TORC1 activity. We found that mutants in PHO84 are hypersensitive to rapamycin and in response to phosphate feeding, generate less phosphorylated ribosomal protein S6 (P-S6) than the WT...
June 13, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28556561/cellular-conditions-that-modulate-the-fungicidal-activity-of-occidiofungin
#4
Chase A Robinson, Connor Denison, Austin Burkenstock, Chris Nutter, Donna M Gordon
AIMS: To identify cellular conditions that significantly alter susceptibility of Saccharomyces cerevisiae, Candida albicans, and Candida glabrata to the antimicrobial peptide, occidiofungin. METHODS AND RESULTS: Genetic and pharmacological approaches were used to determine a role for calcium signaling in occidiofungin sensitivity profiles for Saccharomyces cerevisiae, Candida albicans, and Candida glabrata strains of yeast. Minimum inhibitory concentration (MIC) and drop assays found that extracellular calcium resulted in a four-fold resistance, and this was independent of an intact calmodulin-calcineurin signaling pathway...
May 28, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28512683/messenger-rna-transport-in-the-opportunistic-fungal-pathogen-candida-albicans
#5
REVIEW
Anne E McBride
Candida albicans, a common commensal fungus, can cause disease in immunocompromised hosts ranging from mild mucosal infections to severe bloodstream infections with high mortality rates. The ability of C. albicans cells to switch between a budding yeast form and an elongated hyphal form is linked to pathogenicity in animal models. Hyphal-specific proteins such as cell-surface adhesins and secreted hydrolases facilitate tissue invasion and host cell damage, but the specific mechanisms leading to asymmetric protein localization in hyphae remain poorly understood...
May 16, 2017: Current Genetics
https://www.readbyqxmd.com/read/28512289/a-genetic-screen-in-combination-with-biochemical-analysis-in-saccharomyces-cerevisiae-indicates-that-phenazine-1-carboxylic-acid-is-harmful-to-vesicular-trafficking-and-autophagy
#6
Xiaolong Zhu, Yan Zeng, Xiu Zhao, Shenshen Zou, Ya-Wen He, Yongheng Liang
The environmentally friendly antibiotic phenazine-1-carboxylic acid (PCA) protects plants, mammals and humans effectively against various fungal pathogens. However, the mechanism by which PCA inhibits or kills fungal pathogens is not fully understood. We analyzed the effects of PCA on the growth of two fungal model organisms, Saccharomyces cerevisiae and Candida albicans, and found that PCA inhibited yeast growth in a dose-dependent manner which was inversely dependent on pH. In contrast, the commonly used antibiotic hygromycin B acted in a dose-dependent manner as pH increased...
May 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28501934/cx-4945-the-protein-kinase-ck2-inhibitor-and-anti-cancer-drug-shows-anti-fungal-activity
#7
Maciej Masłyk, Monika Janeczko, Aleksandra Martyna, Konrad Kubiński
CX-4945 is a selective inhibitor of protein kinase CK2 exhibiting clinical significance. Its antitumor properties arise from the abrogation of CK2-mediated pro-survival cellular pathways. The presented data reveal the influence of CX-4945 on the growth of yeast cells showing variable potency against Saccharomyces cerevisiae deletion strains with different contents of CK2 subunits. The catalytic subunit CK2α appears to sensitize yeast to the CX-4945 action. Moreover, the compound suppresses hyphal growth and cell adhesion of Candida albicans, thereby abolishing some hallmarks of invasiveness of the pathogen...
May 13, 2017: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/28493119/co2-sensing-in-fungi-at-the-heart-of-metabolic-signaling
#8
REVIEW
Ronny Martin, Susann Pohlers, Fritz A Mühlschlegel, Oliver Kurzai
Adaptation to the changing environmental CO2 levels is essential for all living cells. In particular, microorganisms colonizing and infecting the human body are exposed to highly variable concentrations, ranging from atmospheric 0.04 to 5% and more in blood and specific host niches. Carbonic anhydrases are highly conserved metalloenzymes that enable fixation of CO2 by its conversion into bicarbonate. This process is not only crucial to ensure the supply of adequate carbon amounts for cellular metabolism, but also contributes to several signaling processes in fungi, including morphology and communication...
May 10, 2017: Current Genetics
https://www.readbyqxmd.com/read/28479022/lathyrol-and-epoxylathyrol-derivatives-modulation-of-cdr1p-and-mdr1p-drug-efflux-transporters-of-candida-albicans-in-saccharomyces-cerevisiae-model
#9
Andreia Mónico, Shweta Nim, Noélia Duarte, Manpreet Kaur Rawal, Rajendra Prasad, Attilio Di Pietro, Maria-José U Ferreira
Macrocyclic diterpenes were previously found to be able to modulate the efflux pump activity of Candida albicans multidrug transporters. Most of these compounds were jatrophanes, but only a few number of lathyrane-type diterpenes was evaluated. Therefore, the aim of this study was to evaluate the ability of nineteen structurally-related lathyrane diterpenes (1-19) to overcome the drug-efflux activity of Cdr1p and Mdr1p transporters of C. albicans, and get some insights on their structure-activity relationships...
July 1, 2017: Bioorganic & Medicinal Chemistry
https://www.readbyqxmd.com/read/28470426/the-yeasts-phosphorelay-systems-a-comparative-view
#10
REVIEW
Griselda Salas-Delgado, Laura Ongay-Larios, Laura Kawasaki-Watanabe, Imelda López-Villaseñor, Roberto Coria
Cells contain signal transduction pathways that mediate communication between the extracellular environment and the cell interior. These pathways control transcriptional programs and posttranscriptional processes that modify cell metabolism in order to maintain homeostasis. One type of these signal transduction systems are the so-called Two Component Systems (TCS), which conduct the transfer of phosphate groups between specific and conserved histidine and aspartate residues present in at least two proteins; the first protein is a sensor kinase which autophosphorylates a histidine residue in response to a stimulus, this phosphate is then transferred to an aspartic residue located in a response regulator protein...
June 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28431022/cpp1-phosphatase-mediated-signaling-crosstalk-between-hog1-and-cek1-mitogen-activated-protein-kinases-is-involved-in-the-phenotypic-transition-in-candida-albicans
#11
Fu-Sheng Deng, Ching-Hsuan Lin
Cellular signaling pathways involved in cell growth and differentiation mediated by mitogen-activated protein kinase (MAPK) cascades have been well characterized in fungi. However, the mechanisms of signaling crosstalk between MAPKs to ensure signaling specificity are largely unknown. Previous work showed that activation of the Candida albicans Cek1 MAPK pathway resulted in opaque cell formation and filamentation, which mirrored the phenotypes to hog1Δ. Additionally, deleting the HOG1 gene stimulated Cek1p...
April 20, 2017: Medical Mycology: Official Publication of the International Society for Human and Animal Mycology
https://www.readbyqxmd.com/read/28375147/crystal-structures-of-hsp104-n-terminal-domains-from-saccharomyces-cerevisiae-and-candida-albicans-suggest-the-mechanism-for-the-function-of-hsp104-in-dissolving-prions
#12
Peng Wang, Jingzhi Li, Clarissa Weaver, Aaron Lucius, Bingdong Sha
Hsp104 is a yeast member of the Hsp100 family which functions as a molecular chaperone to disaggregate misfolded polypeptides. To understand the mechanism by which the Hsp104 N-terminal domain (NTD) interacts with its peptide substrates, crystal structures of the Hsp104 NTDs from Saccharomyces cerevisiae (ScHsp104NTD) and Candida albicans (CaHsp104NTD) have been determined at high resolution. The structures of ScHsp104NTD and CaHsp104NTD reveal that the yeast Hsp104 NTD may utilize a conserved putative peptide-binding groove to interact with misfolded polypeptides...
April 1, 2017: Acta Crystallographica. Section D, Structural Biology
https://www.readbyqxmd.com/read/28373280/heat-shock-protein-104-hsp104-mediated-curing-of-psi-yeast-prions-depends-on-both-psi-conformation-and-the-properties-of-the-hsp104-homologs
#13
Xiaohong Zhao, Ramon Rodriguez, Rebecca E Silberman, Joseph M Ahearn, Sheela Saidha, Kaelyn C Cummins, Evan Eisenberg, Lois E Greene
Prions arise from proteins that have two possible conformations: properly folded and non-infectious or misfolded and infectious. The [PSI(+)] yeast prion, which is the misfolded and self-propagating form of the translation termination factor eRF3 (Sup35), can be cured of its infectious conformation by overexpression of Hsp104, which helps dissolve the prion seeds. This dissolution depends on the trimming activity of Hsp104, which reduces the size of the prion seeds without increasing their number. To further understand the relationship between trimming and curing, trimming was followed by measuring the loss of GFP-labeled Sup35 foci from both strong and weak [PSI(+)] variants; the former variant has more seeds and less soluble Sup35 than the latter...
May 26, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28334124/a-newly-identified-amino-acid-substitution-t123i-in-the-14%C3%AE-demethylase-erg11p-of-candida-albicans-confers-azole-resistance
#14
YongQin Wu, Ning Gao, Cui Li, Jing Gao, ChunMei Ying
The increasing prevalence of azole resistance in Candida albicans poses a growing problem for clinical treatment. Amino acid substitution of the 14α-demethylase (Erg11p) encoded by the ERG11 gene is one of the most common mechanisms involved in azole resistance. Although amino acid substitutions of Erg11p have been observed in many clinical isolates, only a few amino acid substitutions have been confirmed to be related to azole resistance. In this study, by amplifying and sequencing the open reading frame of the ERG11 gene from 55 clinical isolates, we identified 27 fluconazole-resistant isolates that harbor a novel amino acid substitution, T123I, in Erg11p, in addition to the previously described homozygous substitution Y132H...
May 1, 2017: FEMS Yeast Research
https://www.readbyqxmd.com/read/28289726/regulation-of-the-candida-albicans-hypha-inducing-transcription-factor-ume6-by-the-cdk1-cyclins-cln3-and-hgc1
#15
Sigal Mendelsohn, Mariel Pinsky, Ziva Weissman, Daniel Kornitzer
The ability to switch between proliferation as yeast cells and development into hyphae is a hallmark of Candida albicans. The switch to hyphal morphogenesis depends on external inducing conditions, but its efficiency is augmented in stationary-phase cells. Ume6, a transcription factor that is itself transcriptionally induced under hypha-promoting conditions, is both necessary and sufficient for hyphal morphogenesis. We found that Ume6 is regulated posttranslationally by the cell cycle kinase Cdc28/Cdk1, which reduces Ume6 activity via different mechanisms using different cyclins...
March 2017: MSphere
https://www.readbyqxmd.com/read/28261190/fungal-its1-deep-sequencing-strategies-to-reconstruct-the-composition-of-a-26-species-community-and-evaluation-of-the-gut-mycobiota-of-healthy-japanese-individuals
#16
Daisuke Motooka, Kosuke Fujimoto, Reiko Tanaka, Takashi Yaguchi, Kazuyoshi Gotoh, Yuichi Maeda, Yoki Furuta, Takashi Kurakawa, Naohisa Goto, Teruo Yasunaga, Masashi Narazaki, Atsushi Kumanogoh, Toshihiro Horii, Tetsuya Iida, Kiyoshi Takeda, Shota Nakamura
The study of mycobiota remains relatively unexplored due to the lack of sufficient available reference strains and databases compared to those of bacterial microbiome studies. Deep sequencing of Internal Transcribed Spacer (ITS) regions is the de facto standard for fungal diversity analysis. However, results are often biased because of the wide variety of sequence lengths in the ITS regions and the complexity of high-throughput sequencing (HTS) technologies. In this study, a curated ITS database, ntF-ITS1, was constructed...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28260588/utilization-of-size-polymorphism-in-its1-and-its2-regions-for-identification-of-pathogenic-yeast-species
#17
Hossein Khodadadi, Ladan Karimi, Nilufar Jalalizand, Hassan Adin, Hossein Mirhendi
PURPOSE: Despite the existence of a variety of available yeast-identification strategies, easier and more cost-effective methods are required for routine use in clinical laboratories. The internal transcribed spacer (ITS) regions of fungal rRNA genes exhibit variable sizes depending on the yeast species. In the present study, fragment size polymorphism (FSP) analysis of the ITS1 and ITS2 regions for identification of the clinically most important yeast species was assessed. METHODOLOGY: The ITS1 and ITS2 regions of 190 strains, including isolates of 31 standard strains and 159 clinical isolates, were separately PCR amplified with two primer sets: ITS1-ITS2 and ITS3-ITS4...
March 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28250014/the-pivotal-role-of-protein-phosphorylation-in-the-control-of-yeast-central-metabolism
#18
Panayotis Vlastaridis, Athanasios Papakyriakou, Anargyros Chaliotis, Efstratios Stratikos, Stephen G Oliver, Grigorios D Amoutzias
Protein phosphorylation is the most frequent eukaryotic post-translational modification and can act as either a molecular switch or rheostat for protein functions. The deliberate manipulation of protein phosphorylation has great potential for regulating specific protein functions with surgical precision, rather than the gross effects gained by the over/underexpression or complete deletion of a protein-encoding gene. In order to assess the impact of phosphorylation on central metabolism, and thus its potential for biotechnological and medical exploitation, a compendium of highly confident protein phosphorylation sites (p-sites) for the model organism Saccharomyces cerevisiae has been analyzed together with two more datasets from the fungal pathogen Candida albicans Our analysis highlights the global properties of the regulation of yeast central metabolism by protein phosphorylation, where almost half of the enzymes involved are subject to this sort of post-translational modification...
April 3, 2017: G3: Genes—Genomes—Genetics
https://www.readbyqxmd.com/read/28185554/evolution-of-transcriptional-networks-in-yeast-alternative-teams-of-transcriptional-factors-for-different-species
#19
Adriana Muñoz, Daniella Santos Muñoz, Aleksey Zimin, James A Yorke
BACKGROUND: The diversity in eukaryotic life reflects a diversity in regulatory pathways. Nocedal and Johnson argue that the rewiring of gene regulatory networks is a major force for the diversity of life, that changes in regulation can create new species. RESULTS: We have created a method (based on our new "ping-pong algorithm) for detecting more complicated rewirings, where several transcription factors can substitute for one or more transcription factors in the regulation of a family of co-regulated genes...
November 11, 2016: BMC Genomics
https://www.readbyqxmd.com/read/28143980/lipid-signaling-via-pkh1-2-regulates-fungal-co2-sensing-through-the-kinase-sch9
#20
Susann Pohlers, Ronny Martin, Thomas Krüger, Daniela Hellwig, Frank Hänel, Olaf Kniemeyer, Hans Peter Saluz, Patrick Van Dijck, Joachim F Ernst, Axel Brakhage, Fritz A Mühlschlegel, Oliver Kurzai
Adaptation to alternating CO2 concentrations is crucial for all organisms. Carbonic anhydrases-metalloenzymes that have been found in all domains of life-enable fixation of scarce CO2 by accelerating its conversion to bicarbonate and ensure maintenance of cellular metabolism. In fungi and other eukaryotes, the carbonic anhydrase Nce103 has been shown to be essential for growth in air (~0.04% CO2). Expression of NCE103 is regulated in response to CO2 availability. In Saccharomyces cerevisiae, NCE103 is activated by the transcription factor ScCst6, and in Candida albicans and Candida glabrata, it is activated by its homologues CaRca1 and CgRca1, respectively...
January 31, 2017: MBio
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