keyword
MENU ▼
Read by QxMD icon Read
search

Saccharomyces albicans

keyword
https://www.readbyqxmd.com/read/29340656/antagonistic-effect-of-saccharomyces-cerevisiae-ktp-and-issatchenkia-occidentalis-apc-on-hyphal-development-and-adhesion-of-candida-albicans
#1
K Lohith, K A Anu-Appaiah
The morphological transition from yeast to a hyphal form, as well as the adhesion capability to the gastrointestinal tract, are implicated virulent determinant in Candida albicans and could be potential targets for prevention of the opportunistic pathogen. Based on this rationale, two yeast strains Saccharomyces cerevisiae KTP and Issatchenkia occidentalis ApC along with reference strain Saccharomyces boulardii NCDC 363 were screened for the probiotic potential. Characters like pH, temperature, bile, simulated gastrointestinal juice tolerance tests, and Caco-2 cell line adhesion assay were determined in the present study...
January 11, 2018: Medical Mycology: Official Publication of the International Society for Human and Animal Mycology
https://www.readbyqxmd.com/read/29331410/implementing-crispr-cas-technologies-in-conventional-and-non-conventional-yeasts-current-state-and-future-prospects
#2
REVIEW
Hana Raschmanova, Astrid Weninger, Anton Glieder, Karin Kovar, Thomas Vogl
Within five years, the CRISPR-Cas system has emerged as the dominating tool for genome engineering, while also changing the speed and efficiency of metabolic engineering in conventional (Saccharomyces cerevisiae and Schizosaccharomyces pombe) and non-conventional (Yarrowia lipolytica, Pichia pastoris syn. Komagataella phaffii, Kluyveromyces lactis, Candida albicans and C. glabrata) yeasts. Especially in S. cerevisiae, an extensive toolbox of advanced CRISPR-related applications has been established, including crisprTFs and gene drives...
January 10, 2018: Biotechnology Advances
https://www.readbyqxmd.com/read/29310582/identification-of-uncommon-oral-yeasts-from-cancer-patients-by-maldi-tof-mass-spectrometry
#3
Narges Aslani, Ghasem Janbabaei, Mahdi Abastabar, Jacques F Meis, Mahasti Babaeian, Sadegh Khodavaisy, Teun Boekhout, Hamid Badali
BACKGROUND: Opportunistic infections due to Candida species occur frequently in cancer patients because of their inherent immunosuppression. The aim of the present study was to investigate the epidemiology of yeast species from the oral cavity of patients during treatment for oncological and haematological malignancies. METHODS: MALDI-TOF was performed to identify yeasts isolated from the oral cavity of 350 cancer patients. Moreover, antifungal susceptibility testing was performed in according to CLSI guidelines (M27-A3)...
January 8, 2018: BMC Infectious Diseases
https://www.readbyqxmd.com/read/29249661/patterns-of-genomic-variation-in-the-opportunistic-pathogen-candida-glabrata-suggest-the-existence-of-mating-and-a-secondary-association-with-humans
#4
Laia Carreté, Ewa Ksiezopolska, Cinta Pegueroles, Emilia Gómez-Molero, Ester Saus, Susana Iraola-Guzmán, Damian Loska, Oliver Bader, Cecile Fairhead, Toni Gabaldón
Candida glabrata is an opportunistic fungal pathogen that ranks as the second most common cause of systemic candidiasis. Despite its genus name, this yeast is more closely related to the model yeast Saccharomyces cerevisiae than to other Candida pathogens, and hence its ability to infect humans is thought to have emerged independently. Moreover, C. glabrata has all the necessary genes to undergo a sexual cycle but is considered an asexual organism due to the lack of direct evidence of sexual reproduction. To reconstruct the recent evolution of this pathogen and find footprints of sexual reproduction, we assessed genomic and phenotypic variation across 33 globally distributed C...
December 8, 2017: Current Biology: CB
https://www.readbyqxmd.com/read/29248994/the-phosphorelay-signal-transduction-system-in-candida-glabrata-an-in-silico-analysis
#5
Natalee Carapia-Minero, Juan Arturo Castelán-Vega, Néstor Octavio Pérez, Aída Verónica Rodríguez-Tovar
Signaling systems allow microorganisms to sense and respond to different stimuli through the modification of gene expression. The phosphorelay signal transduction system in eukaryotes involves three proteins: a sensor protein, an intermediate protein and a response regulator, and requires the transfer of a phosphate group between two histidine-aspartic residues. The SLN1-YPD1-SSK1 system enables yeast to adapt to hyperosmotic stress through the activation of the HOG1-MAPK pathway. The genetic sequences available from Saccharomyces cerevisiae were used to identify orthologous sequences in Candida glabrata, and putative genes were identified and characterized by in silico assays...
December 16, 2017: Journal of Molecular Modeling
https://www.readbyqxmd.com/read/29242833/put3-positively-regulates-proline-utilization-in-candida-albicans
#6
Walters Aji Tebung, Raha Parvizi Omran, Debra L Fulton, Joachim Morschhäuser, Malcolm Whiteway
The zinc cluster transcription factor Put3 was initially characterized in Saccharomyces cerevisiae as the transcriptional activator of PUT1 and PUT2, two genes acting early in the proline assimilation pathway. We have used phenotypic studies, transcription profiling, and chromatin immunoprecipitation with microarray technology (ChIP-chip) to establish that unlike S. cerevisiae, which only uses proline as a nitrogen source, Candida albicans can use proline as a nitrogen source, a carbon source, or a source of both nitrogen and carbon...
November 2017: MSphere
https://www.readbyqxmd.com/read/29236852/antifungal-activities-of-the-essential-oil-and-its-fractions-rich-in-sesquiterpenes-from-leaves-of-casearia-sylvestris-sw
#7
Flaviane G Pereira, Ronaldo Marquete, Levy T Domingos, Marco E N Rocha, Antonio Ferreira-Pereira, Elisabeth Mansur, Davyson L Moreira
Casearia genus (Salicaceae) is found in sub-tropical and tropical regions of the world and comprises about 160-200 species. It is a medicinal plant used in South America, also known as "guaçatonga", "erva-de-tiú", "cafezinho-do-mato". In Brazil, there are about 48 species and 12 are registered in the State of Rio de Janeiro, including Casearia sylvestris Sw. There are many studies related to the chemical profile and cytotoxic activities of extracts from these plants, although few studies about the antifungal potential of the essential oil have been reported...
December 11, 2017: Anais da Academia Brasileira de Ciências
https://www.readbyqxmd.com/read/29217144/histatin-5-induces-the-reversal-of-pdr5p-mediated-fluconazole-resistance-in-saccharomyces%C3%A2-cerevisae
#8
J A da Rocha Curvelo, L F Reis de Sá, D C Clemente de Moraes, R M Soares, A Ferreira-Pereira
BACKGROUND: Candidiasis is a major opportunistic fungal infection in humans. The low number of antifungal drugs available to treat Candida infections and the increasing incidence of multidrug resistant (MDR) strains point to an urgent need of identifying new therapeutic options. The role of salivary components can provide insights for the development of new methodologies of control. OBJECTIVE: The aim of this study was to evaluate the ability of histatin-5, a constitutive immunological peptide present in saliva, in reversing fungal MDR phenotype, using a resistant Saccharomyces cerevisiae strain as model of study...
December 4, 2017: Journal de Mycologie Médicale
https://www.readbyqxmd.com/read/29178920/the-gut-mycobiome-of-the-human-microbiome-project-healthy-cohort
#9
Andrea K Nash, Thomas A Auchtung, Matthew C Wong, Daniel P Smith, Jonathan R Gesell, Matthew C Ross, Christopher J Stewart, Ginger A Metcalf, Donna M Muzny, Richard A Gibbs, Nadim J Ajami, Joseph F Petrosino
BACKGROUND: Most studies describing the human gut microbiome in healthy and diseased states have emphasized the bacterial component, but the fungal microbiome (i.e., the mycobiome) is beginning to gain recognition as a fundamental part of our microbiome. To date, human gut mycobiome studies have primarily been disease centric or in small cohorts of healthy individuals. To contribute to existing knowledge of the human mycobiome, we investigated the gut mycobiome of the Human Microbiome Project (HMP) cohort by sequencing the Internal Transcribed Spacer 2 (ITS2) region as well as the 18S rRNA gene...
November 25, 2017: Microbiome
https://www.readbyqxmd.com/read/29174402/commensal-fungi-recapitulate-the-protective-benefits-of-intestinal-bacteria
#10
Tony T Jiang, Tzu-Yu Shao, W X Gladys Ang, Jeremy M Kinder, Lucien H Turner, Giang Pham, Jordan Whitt, Theresa Alenghat, Sing Sing Way
Commensal intestinal microbes are collectively beneficial in preventing local tissue injury and augmenting systemic antimicrobial immunity. However, given the near-exclusive focus on bacterial species in establishing these protective benefits, the contributions of other types of commensal microbes remain poorly defined. Here, we show that commensal fungi can functionally replace intestinal bacteria by conferring protection against injury to mucosal tissues and positively calibrating the responsiveness of circulating immune cells...
November 18, 2017: Cell Host & Microbe
https://www.readbyqxmd.com/read/29163439/phosphomannosylation-and-the-functional-analysis-of-the-extended-candida-albicans-mnn4-like-gene-family
#11
Roberto J González-Hernández, Kai Jin, Marco J Hernández-Chávez, Diana F Díaz-Jiménez, Elías Trujillo-Esquivel, Diana M Clavijo-Giraldo, Alma K Tamez-Castrellón, Bernardo Franco, Neil A R Gow, Héctor M Mora-Montes
Phosphomannosylation is a modification of cell wall proteins that occurs in some species of yeast-like organisms, including the human pathogen Candida albicans. These modified mannans confer a negative charge to the wall, which is important for the interactions with phagocytic cells of the immune systems and cationic antimicrobial peptides. In Saccharomyces cerevisiae, the synthesis of phosphomannan relies on two enzymes, the phosphomannosyltransferase Ktr6 and its positive regulator Mnn4. However, in C. albicans, at least three phosphomannosyltransferases, Mnn4, Mnt3 and Mnt5, participate in the addition of phosphomannan...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29154829/a-role-for-candida-albicans-superoxide-dismutase-enzymes-in-glucose-signaling
#12
Chynna N Broxton, Bixi He, Vincent M Bruno, Valeria C Culotta
The Saccharomyces cerevisiae and Candida albicans yeasts have evolved to differentially use glucose for fermentation versus respiration. S. cerevisiae is Crabtree positive, where glucose represses respiration and promotes fermentation, while the opportunistic fungal pathogen C. albicans is Crabtree negative and does not repress respiration with glucose. We have previously shown that glucose control in S. cerevisiae involves the antioxidant enzyme Cu/Zn superoxide dismutase (SOD1), where H2O2 generated by SOD1 stabilizes the casein kinase YCK1 for glucose sensing...
November 14, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29152581/the-candida-albicans-tor-activating-gtpases-gtr1-and-rhb1-coregulate-starvation-responses-and-biofilm-formation
#13
Peter R Flanagan, Ning-Ning Liu, Darren J Fitzpatrick, Karsten Hokamp, Julia R Köhler, Gary P Moran
Target of rapamycin complex 1 (TORC1) is an essential regulator of metabolism in eukaryotic cells and in the fungal pathogen Candida albicans regulates morphogenesis and nitrogen acquisition. Gtr1 encodes a highly conserved GTPase that in Saccharomyces cerevisiae regulates nitrogen sensing and TORC1 activation. Here, we characterize the role of C. albicans GTR1 in TORC1 activation and compare it with the previously characterized GTPase Rhb1. A homozygous gtr1/gtr1 mutant exhibited impaired TORC1-mediated phosphorylation of ribosomal protein S6 and increased susceptibility to rapamycin...
November 2017: MSphere
https://www.readbyqxmd.com/read/29146491/respiratory-stress-in-mitochondrial-electron-transport-chain-complex-mutants-of-candida-albicans-activates-snf1-kinase-response
#14
Pengyi Zhang, Hongbin Li, Jie Cheng, April Y Sun, Liqing Wang, Gordana Mircevska, Richard Calderone, Dongmei Li
We have previously established that mitochondrial Complex I (CI) mutants of Candida albicans display reduced oxygen consumption, decreased ATP production, and increased reactive oxidant species (ROS) during cell growth. Using the Seahorse XF96 analyzer, the energetic phenotypes of Electron Transport Chain (ETC) complex mutants are further characterized in the current study. The underlying regulation of energetic changes in these mutants is determined in glucose and non-glucose conditions when compared to wild type (WT) cells...
November 13, 2017: Fungal Genetics and Biology: FG & B
https://www.readbyqxmd.com/read/29139202/lego-inspired-drug-design-unveiling-of-the-novel-class-of-benzo-d-thiazole-containing-a-3-4-dihydroxyphenyl-moiety-as-plasma-membrane-h-atpase-inhibitors
#15
Truong Thanh Tung, Dao Trong Tuan, Marta Grifell Junyent, Michael Palmgren, Thomas Günther-Pomorski, Anja Thoe Fuglsang, Søren Brøgger Christensen, John Nielsen
The fungal plasma membrane H+-ATPase (Pma1p) is a potential target for the discovery of new antifungal agents. Surprisingly, no structure-activity relationship studies for small molecules targeting Pma1p have been reported. Herein, we disclose a LEGO-inspired fragment assembly strategy for design, synthesis and discovery of benzo[d]thiazoles containing a 3,4-dihydroxyphenyl moiety as potential Pma1p inhibitors. A series of 2-(benzo[d]thiazol-2-ylthio)-1-(3,4-dihydroxyphenyl)ethanones was found to inhibit Pma1p with the most potent IC50 of 8 µM in an in vitro plasma membrane H+-ATPase assay...
November 15, 2017: ChemMedChem
https://www.readbyqxmd.com/read/29129987/increasing-the-fungicidal-action-of-amphotericin-b-by-inhibiting-the-nitric-oxide-dependent-tolerance-pathway
#16
Kim Vriens, Phalguni Tewari Kumar, Caroline Struyfs, Tanne L Cools, Pieter Spincemaille, Tadej Kokalj, Belém Sampaio-Marques, Paula Ludovico, Jeroen Lammertyn, Bruno P A Cammue, Karin Thevissen
Amphotericin B (AmB) induces oxidative and nitrosative stresses, characterized by production of reactive oxygen and nitrogen species, in fungi. Yet, how these toxic species contribute to AmB-induced fungal cell death is unclear. We investigated the role of superoxide and nitric oxide radicals in AmB's fungicidal activity in Saccharomyces cerevisiae, using a digital microfluidic platform, which enabled monitoring individual cells at a spatiotemporal resolution, and plating assays. The nitric oxide synthase inhibitor L-NAME was used to interfere with nitric oxide radical production...
2017: Oxidative Medicine and Cellular Longevity
https://www.readbyqxmd.com/read/29119849/graphene-oxide-significantly-inhibits-cell-growth-at-sublethal-concentrations-by-causing-extracellular-iron-deficiency
#17
Qilin Yu, Bing Zhang, Jianrong Li, Tingting Du, Xiao Yi, Mingchun Li, Wei Chen, Pedro J J Alvarez
Graphene oxide (GO)-based materials are increasingly being used in medical materials and consumer products. However, their sublethal effects on biological systems are poorly understood. Here, we report that GO (at 10 to 160 mg/L) induced significant inhibitory effects on the growth of different unicellular organisms, including eukaryotes (i.e. Saccharomyces cerevisiae, Candida albicans, and Komagataella pastoris) and prokaryotes (Pseudomonas fluorescens). Growth inhibition could not be explained by commonly reported cytotoxicity mechanisms such as plasma membrane damage or oxidative stress...
November 9, 2017: Nanotoxicology
https://www.readbyqxmd.com/read/29101061/antimicrobial-efficacy-of-drug-blended-biosynthesized-colloidal-gold-nanoparticles-from-justicia-glauca-against-oral-pathogens-a-nanoantibiotic-approach
#18
Rayappan Emmanuel, Muthupandian Saravanan, Muhammad Ovais, Sethuramasamy Padmavathy, Zabta Khan Shinwari, Periyakaruppan Prakash
Antimicrobial resistance is a challenging task for researchers to develop new strategies. Green synthesis of AuNPs is an eco-friendly approach, which can be utilized in the microbistatic and microbicidal activities. The current study is focused on Justicia glauca (aqueous leaf extract) mediated AuNPs synthesis at room temperature by treating chloroaurate ions, that shows an antagonistic effect with Azithromycin (AZM) and Clarithromycin (CLR) antibiotics against oral pathogenic bacteria and fungi (Micrococcus luteus, Bacillus subtilis, Staphylococcus aureus, Streptococcus mutans, Lactobacillus acidophilus, Escherichia coli, Pseudomonas aeruginosa, Saccharomyces cerevisiae and Candida albicans)...
October 31, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/29069410/protein-kinase-c-in-fungi-more-than-just-cell-wall-integrity
#19
Jürgen J Heinisch, Rosaura Rodicio
Human protein kinase C (PKC) isoforms have been implicated in diseases such as Alzheimer's, diabetes, and cancers. In contrast to mammals, which have at least 9 genes, fungi have only one or two. The yeast Saccharomyces cerevisiae produces only a single Pkc1 and is employed in the study of specific human isozymes, including their susceptibility to pharmacological drugs. Vice versa , the domain structure and regulation of yeast and other fungal PKCs yield insights into the function of human isozymes. Therefore, human PKCs are briefly reviewed herein and related to the yeast enzyme...
October 23, 2017: FEMS Microbiology Reviews
https://www.readbyqxmd.com/read/28979764/saccharomyces-cerevisiae-show-low-levels-of-traversal-across-the-human-blood-brain-barrier-in-vitro
#20
Roberto Pérez-Torrado, Amparo Querol
BACKGROUND:   Saccharomyces cerevisiae is generally considered safe, and is involved in the production of many types of foods and dietary supplements. However, some isolates, which are genetically related to strains used in brewing and baking, have shown virulent traits, being able to produce infections in humans, mainly in immunodeficient patients. This can lead to systemic infections in humans. METHODS: In this work, we studied S. cerevisiae isolates in an in vitro human blood brain barrier model, comparing their behaviour with that of several strains of the related pathogens Candida glabrata and Candida albicans...
2017: F1000Research
keyword
keyword
85575
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"