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Saccharomyces albicans

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https://www.readbyqxmd.com/read/28919409/solasodine-3-o-%C3%AE-d-glucopyranoside-is-hydrolyzed-by-a-membrane-glucosidase-into-active-molecule-solasodine-against-candida-albicans
#1
Wenqiang Chang, Ying Li, Sha Zheng, Ming Zhang, Yanhui Gao, Hongxiang Lou
Antifungal activity of some natural molecules can be abated or blocked by efflux pumps in Candida albicans, which restricts the discovery of potential antifungal agents. Here we found that the steroidal alkaloid solasodine is active against C. albicans efflux pump-deficient strains but inert towards the wild type. However, the glucosylated solasodine-3-O-β-D-glucopyranoside exhibits antifungal activity towards the wild type strain. Further investigation revealed that the entry of solasodine into C. albicans cells is blocked by efflux pumps...
September 15, 2017: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/28893786/novel-cell-killing-mechanisms-of-hydroxyurea-and-the-implication-towards-combination-therapy-for-the-treatment-of-fungal-infections
#2
Amanpreet Singh, Ameeta Agarwal, Yong-Jie Xu
We have previously reported that an erg11 mutation affecting ergosterol synthesis and a hem13 mutation in the heme synthesis pathway significantly sensitize the fission yeast Schizosaccahromyces pombe to hydroxyurea (HU) (1, 2). Here we show that treatment with the inhibitors of Erg11 and the heme biosynthesis phenocopies the two mutations in sensitizing wild type cells to HU. Importantly, HU synergistically interacts with the heme biosynthesis inhibitor sampangine and several Erg11 inhibitors, the antifungal azoles, in causing cell lethality...
September 11, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28852966/characterization-of-the-fungal-flora-of-dolo-a-traditional-fermented-beverage-of-burkina-faso-using-maldi-tof-mass-spectrometry
#3
Bamba Sanata, Zida Adama, Sangaré Ibrahim, Sondo Apoline, Cissé Mamoudou, Sirima Constant, Guiguemdé T Robert, Hennequin Christophe
A cross-sectional study was conducted in Bobo-Dioulasso, Burkina Faso, to identify the yeast diversity associated with the manufacture of dolo, a traditional fermented beverage of Burkina Faso. From sixty specimens spread onto chromogenic medium plates, sixty-two strains were isolated then identified using MALDI-TOF analysis. Seven yeast species were identified, Saccharomyces cerevisiae (39%) followed by Pichia manshurica (18%) being the most frequent. Forty-three percent of the samples contained Candida species, notably Candida albicans...
August 29, 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28829379/phosphate-acquisition-and-virulence-in-human-fungal-pathogens
#4
REVIEW
Mélanie Ikeh, Yasmin Ahmed, Janet Quinn
The ability of pathogenic fungi to acquire essential macro and micronutrients during infection is a well-established virulence trait. Recent studies in the major human fungal pathogens Candida albicans and Cryptococcus neoformans have revealed that acquisition of the essential macronutrient, phosphate, is essential for virulence. The phosphate sensing and acquisition pathway in fungi, known as the PHO pathway, has been extensively characterized in the model yeast Saccharomyces cerevisiae. In this review, we highlight recent advances in phosphate sensing and signaling mechanisms, and use the S...
August 22, 2017: Microorganisms
https://www.readbyqxmd.com/read/28810704/yeast-kch1-and-kch2-membrane-proteins-play-a-pleiotropic-role-in-membrane-potential-establishment-and-monovalent-cation-homeostasis-regulation
#5
Kristina Felcmanova, Petra Neveceralova, Hana Sychrova, Olga Zimmermannova
The Kch1 and Kch2 plasma-membrane proteins were identified in Saccharomyces cerevisiae as being essential for the activation of a high-affinity Ca2+ influx system. We searched for Kch proteins roles in the maintenance of cation homeostasis and tested the effect of kch1 and/or kch2 deletions on various physiological parameters. Compared to wild-type, kch1 kch2 mutant cells were smaller, relatively hyperpolarised, grew better under limited K+ conditions and exhibited altered growth in the presence of monovalent cations...
August 1, 2017: FEMS Yeast Research
https://www.readbyqxmd.com/read/28801230/a-mitochondrial-proteomics-view-of-complex-i-deficiency-in-candida-albicans
#6
Xiaodong She, Pengyi Zhang, Ying Gao, Lulu Zhang, Qiong Wang, Hui Chen, Richard Calderone, Weida Liu, Dongmei Li
Proteomic analyses were carried out on isolated mitochondrial samples of C. albicans from gene-deleted mutants (nuo1Δ, nuo2Δ and goa1Δ) as well as the parental strain in order to better understand the contribution of these three fungal-specific mitochondrial ETC complex I (CI) subunits to cellular activities. Herein, we identify 2333 putative proteins from four strains, in which a total of 663 proteins (28.5%) are putatively located in mitochondria. Comparison of protein abundances between mutants and the parental strain reveal 146 differentially-expressed proteins, of which 78 are decreased and 68 are increased in at least one mutant...
August 8, 2017: Mitochondrion
https://www.readbyqxmd.com/read/28774774/saccharomyces-cerevisiae-ktr4-ktr5-and-ktr7-encode-mannosyltransferases-differentially-involved-in-the-n-and-o-linked-glycosylation-pathways
#7
Nahúm V Hernández, Luz A López-Ramírez, Diana F Díaz-Jiménez, Erika Mellado-Mojica, Iván Martínez-Duncker, Mercedes G López, Héctor M Mora-Montes
Saccharomyces cerevisiae is a model to understand basic aspects of protein glycosylation pathways. Although these metabolic routes have been thoroughly studied, there are still knowledge gaps; among them, the role of the MNT1/KRE2 gene family. This family is composed of nine members, with only six functionally characterized. The enzymes Ktr1, Ktr3, and Mnt1/Kre2 have overlapping activities in both O-linked and N-linked glycan synthesis; while Ktr2 and Yur1 participate exclusively in the elongation of the N-linked glycan outer chain...
July 31, 2017: Research in Microbiology
https://www.readbyqxmd.com/read/28764751/the-in-vitro-effects-of-interferon-gamma-alone-or-in-combination-with-amphotericin-b-tested-against-the-pathogenic-fungi-candida-albicans-and-aspergillus-fumigatus
#8
Moufid El-Khoury, Rogine Ligot, Simon Mahoney, Colin M Stack, Gabriel G Perrone, C Oliver Morton
OBJECTIVE: Recent studies into the antifungal activity of NK-cells against the Aspergillus fumigatus have presented differing accounts on their mode of antifungal activity. One of these mechanisms proposed that NK-cells may kill the fungus via the direct effects of exposure to Interferon gamma (IFN-γ). RESULTS: In this study we investigated the direct antifungal effects of recombinant human IFN-γ against a range of pathogenic fungi by measuring cellular damage using an XTT-based assay and cell viability through plate counts...
August 1, 2017: BMC Research Notes
https://www.readbyqxmd.com/read/28741338/fungi-of-the-genus-rhodotorula-isolated-from-the-oral-cavity-of-oncologic-patients-with-colorectal-cancer
#9
Piotr Troska, Ewa Sucharzewska, Maria Dynowska, Elżbieta Ejdys
This paper concerns the frequency of occurrence and enzymatic activity of fungi of the genus Rhodotorula isolated from the oral cavity of patients with colorectal cancer. Swabs from the oral cavity were subjected to standard mycological diagnostic procedures; enzymatic activity of the fungi was evaluated using and API Zym test by BioMereux. The fungi of the genus Rhodotorula were noted in 22.5% positive isolates originating from women and 25.7% positive isolates originating from men. They included mono-species isolates of: Rh...
2017: Annals of Parasitology
https://www.readbyqxmd.com/read/28725151/chemical-constituents-of-helichrysum-italicum-roth-g-don-essential-oil-and-their-antimicrobial-activity-against-gram-positive-and-gram-negative-bacteria-filamentous-fungi-and-candida-albicans
#10
Bouzid Djihane, Nouioua Wafa, Soltani Elkhamssa, De Haro Juan Pedro, Angeles Esteban Maria, Zerroug Mohamed Mihoub
The aerial parts of Helichrysum italicum (Roth) G. Don were subjected to hydrodistillation to obtain essential oils which had been analyzed by gas chromatography and gas chromatography coupled with mass spectrometry and tested for antimicrobial activity against 12 bacteria, two yeasts and four fungi by agar diffusion method. The essential oil yielded 0.44% (v/w) and 67 compounds accounting for 99.24% of the oil were identified with a high content of oxygenated sesquiterpenes (61.42%). The most oxygenated sesquiterpene compounds were α-Cedrene (13...
July 2017: Saudi Pharmaceutical Journal: SPJ: the Official Publication of the Saudi Pharmaceutical Society
https://www.readbyqxmd.com/read/28720726/mitochondrial-cochaperone-mge1-is-involved-in-regulating-susceptibility-to-fluconazole-in-saccharomyces-cerevisiae-and-candida-species
#11
Liesbeth Demuyser, Erwin Swinnen, Alessandro Fiori, Beatriz Herrera-Malaver, Kevin Vestrepen, Patrick Van Dijck
MGE1 encodes a yeast chaperone involved in Fe-S cluster metabolism and protein import into the mitochondria. In this study, we identified MGE1 as a multicopy suppressor of susceptibility to the antifungal fluconazole in the model yeast Saccharomyces cerevisiae We demonstrate that this phenomenon is not exclusively dependent on the integrity of the mitochondrial DNA or on the presence of the drug efflux pump Pdr5. Instead, we show that the increased dosage of Mge1 plays a protective role by retaining increased amounts of ergosterol upon fluconazole treatment...
July 18, 2017: MBio
https://www.readbyqxmd.com/read/28720316/pigeons-and-their-droppings-as-reservoirs-of-candida-and-other-zoonotic-yeasts
#12
Inmaculada Rosario Medina, Lorena Román Fuentes, Miguel Batista Arteaga, Fernando Real Valcárcel, Félix Acosta Arbelo, Daniel Padilla Del Castillo, Soraya Déniz Suárez, Otilia Ferrer Quintana, Belinda Vega Gutiérrez, Freddy Silva Sergent, Begoña Acosta-Hernández
BACKGROUND: The importance of pigeons as reservoirs and carriers of Cryptococcus neoformans and other species of this genus is well-known; however, less is known about their role as reservoirs and carriers of other yeasts that impact public health. AIMS: The present study was performed on Gran Canaria Island to define yeasts other than Cryptococcus spp. that have been reported to impact public health and which could be carried by pigeons. METHODS: Samples were obtained from 83 pigeon lofts (Columba livia); moreover, 331 crop samples, 331 cloacal samples and 174 dropping samples were collected...
July 15, 2017: Revista Iberoamericana de Micología
https://www.readbyqxmd.com/read/28702011/bridging-the-gap-to-non-toxic-fungal-control-lupinus-derived-blad-containing-oligomer-as-a-novel-candidate-to-combat-human-pathogenic-fungi
#13
Ana M Pinheiro, Alexandra Carreira, Thomas A K Prescott, Ricardo B Ferreira, Sara A Monteiro
The lack of antifungal drugs with novel modes of action reaching the clinic is a serious concern. Recently a novel antifungal protein referred to as Blad-containing oligomer (BCO) has received regulatory approval as an agricultural antifungal agent. Interestingly its spectrum of antifungal activity includes human pathogens such as Candida albicans, however, its mode of action has yet to be elucidated. Here we demonstrate that BCO exerts its antifungal activity through inhibition of metal ion homeostasis which results in apoptotic cell death in C...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28701466/methionine-synthase-is-localized-to-the-nucleus-in-pichia-pastoris-and-candida-albicans-and-to-the-cytoplasm-in-saccharomyces-cerevisiae
#14
Umakant Sahu, Vinod K H Rajendra, Shankar S Kapnoor, Raghu Bhagavat, Nagasuma Chandra, Pundi N Rangarajan
Methionine synthase (MS) catalyzes methylation of homocysteine, the last step in the biosynthesis of methionine, which is essential for the regeneration of tetrahydrofolate and biosynthesis of S-adenosylmethionine. Here, we report that MS is localized to the nucleus of Pichia pastoris and Candida albicans but is cytoplasmic in Saccharomyces cerevisiae The P. pastoris strain carrying a deletion of the MET6 gene encoding MS (Ppmet6) exhibits methionine as well as adenine auxotrophy indicating that MS is required for methionine as well as adenine biosynthesis...
September 8, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28684792/fungus-derived-hydroxyl-radicals-kill-hepatic-cells-by-enhancing-nuclear-transglutaminase
#15
Ronak Shrestha, Rajan Shrestha, Xian-Yang Qin, Ting-Fang Kuo, Yugo Oshima, Shun Iwatani, Ryutaro Teraoka, Keisuke Fujii, Mitsuko Hara, Mengqian Li, Azusa Takahashi-Nakaguchi, Hiroji Chibana, Jun Lu, Muyi Cai, Susumu Kajiwara, Soichi Kojima
We previously reported the importance of induced nuclear transglutaminase (TG) 2 activity, which results in hepatic cell death, in ethanol-induced liver injury. Here, we show that co-incubation of either human hepatic cells or mouse primary hepatocytes derived from wild-type but not TG2(-/-) mice with pathogenic fungi Candida albicans and C. glabrata, but not baker's yeast Saccharomyces cerevisiae, induced cell death in host cells by enhancing cellular, particularly nuclear, TG activity. Further pharmacological and genetic approaches demonstrated that this phenomenon was mediated partly by the production of reactive oxygen species (ROS) such as hydroxyl radicals, as detected by a fluorescent probe and electron spin resonance...
July 6, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28674054/identification-and-mode-of-action-of-a-plant-natural-product-targeting-human-fungal-pathogens
#16
Stéphane Dorsaz, Tiia Snäkä, Quentin Favre-Godal, Pierre Maudens, Nathalie Boulens, Pascal Furrer, Samad Nejad Ebrahimi, Matthias Hamburger, Eric Allémann, Katia Gindro, Emerson Ferreira Queiroz, Howard Riezman, Jean-Luc Wolfender, Dominique Sanglard
Candida albicans is a major cause of fungal diseases in humans, and its resistance to available drugs is of concern. In an attempt to identify novel antifungal agents, we initiated a small-scale screening of a library of 199 natural plant compounds (i.e., natural products [NPs]). In vitro susceptibility profiling experiments identified 33 NPs with activity against C. albicans (MIC50s ≤ 32 μg/ml). Among the selected NPs, the sterol alkaloid tomatidine was further investigated. Tomatidine originates from the tomato (Solanum lycopersicum) and exhibited high levels of fungistatic activity against Candida species (MIC50s ≤ 1 μg/ml) but no cytotoxicity against mammalian cells...
September 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28642757/the-fungal-pathogen-candida-glabrata-does-not-depend-on-surface-ferric-reductases-for-iron-acquisition
#17
Franziska Gerwien, Abu Safyan, Stephanie Wisgott, Sascha Brunke, Lydia Kasper, Bernhard Hube
Iron acquisition is a crucial virulence determinant for many bacteria and fungi, including the opportunistic fungal pathogens Candida albicans and C. glabrata. While the diverse strategies used by C. albicans for obtaining iron from the host are well-described, much less is known about the acquisition of this micronutrient from host sources by C. glabrata - a distant relative of C. albicans with closer evolutionary ties to Saccharomyces cerevisiae, which nonetheless causes severe clinical symptoms in humans...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28580409/data-from-proteome-analysis-of-lasiodiplodia-theobromae-botryosphaeriaceae
#18
Carla C Uranga, Majid Ghassemian, Rufina Hernández-Martínez
Trunk disease fungi are a global problem affecting many economically important fruiting trees. The Botryosphaeriaceae are a family of trunk disease fungi that require detailed biochemical characterization in order to gain insight into their pathogenicity. The application of a modified Folch extraction to protein extraction from the Botryosphaeriaceae Lasiodiplodia theobromae generated an unprecedented data set of protein identifications from fragmentation analysis and de novo peptide sequencing of its proteome...
August 2017: Data in Brief
https://www.readbyqxmd.com/read/28577425/denaturing-gradient-gel-electrophoresis-profiles-of-bacteria-from-the-saliva-of-twenty-four-different-individuals-form-clusters-that-showed-no-relationship-to-the-yeasts-present
#19
Manjula M Weerasekera, Chris H Sissons, Lisa Wong, Sally A Anderson, Ann R Holmes, Richard D Cannon
OBJECTIVES: The aim was to investigate the relationship between groups of bacteria identified by cluster analysis of the DGGE fingerprints and the amounts and diversity of yeast present. METHODS: Bacterial and yeast populations in saliva samples from 24 adults were analysed using denaturing gradient gel electrophoresis (DGGE) of the bacteria present and by yeast culture. RESULTS: Eubacterial DGGE banding patterns showed considerable variation between individuals...
October 2017: Archives of Oral Biology
https://www.readbyqxmd.com/read/28566496/phosphate-is-the-third-nutrient-monitored-by-tor-in-candida-albicans-and-provides-a-target-for-fungal-specific-indirect-tor-inhibition
#20
Ning-Ning Liu, Peter R Flanagan, Jumei Zeng, Niketa M Jani, Maria E Cardenas, Gary P Moran, Julia R Köhler
The Target of Rapamycin (TOR) pathway regulates morphogenesis and responses to host cells in the fungal pathogen Candida albicans Eukaryotic Target of Rapamycin complex 1 (TORC1) induces growth and proliferation in response to nitrogen and carbon source availability. Our unbiased genetic approach seeking unknown components of TORC1 signaling in C. albicans revealed that the phosphate transporter Pho84 is required for normal TORC1 activity. We found that mutants in PHO84 are hypersensitive to rapamycin and in response to phosphate feeding, generate less phosphorylated ribosomal protein S6 (P-S6) than the WT...
June 13, 2017: Proceedings of the National Academy of Sciences of the United States of America
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