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Saccharomyces albicans

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https://www.readbyqxmd.com/read/29163439/phosphomannosylation-and-the-functional-analysis-of-the-extended-candida-albicans-mnn4-like-gene-family
#1
Roberto J González-Hernández, Kai Jin, Marco J Hernández-Chávez, Diana F Díaz-Jiménez, Elías Trujillo-Esquivel, Diana M Clavijo-Giraldo, Alma K Tamez-Castrellón, Bernardo Franco, Neil A R Gow, Héctor M Mora-Montes
Phosphomannosylation is a modification of cell wall proteins that occurs in some species of yeast-like organisms, including the human pathogen Candida albicans. These modified mannans confer a negative charge to the wall, which is important for the interactions with phagocytic cells of the immune systems and cationic antimicrobial peptides. In Saccharomyces cerevisiae, the synthesis of phosphomannan relies on two enzymes, the phosphomannosyltransferase Ktr6 and its positive regulator Mnn4. However, in C. albicans, at least three phosphomannosyltransferases, Mnn4, Mnt3 and Mnt5, participate in the addition of phosphomannan...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29154829/a-role-for-candida-albicans-superoxide-dismutase-enzymes-in-glucose-signaling
#2
Chynna N Broxton, Bixi He, Vincent M Bruno, Valeria C Culotta
The Saccharomyces cerevisiae and Candida albicans yeasts have evolved to differentially use glucose for fermentation versus respiration. S. cerevisiae is Crabtree positive, where glucose represses respiration and promotes fermentation, while the opportunistic fungal pathogen C. albicans is Crabtree negative and does not repress respiration with glucose. We have previously shown that glucose control in S. cerevisiae involves the antioxidant enzyme Cu/Zn superoxide dismutase (SOD1), where H2O2 generated by SOD1 stabilizes the casein kinase YCK1 for glucose sensing...
November 14, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29152581/the-candida-albicans-tor-activating-gtpases-gtr1-and-rhb1-coregulate-starvation-responses-and-biofilm-formation
#3
Peter R Flanagan, Ning-Ning Liu, Darren J Fitzpatrick, Karsten Hokamp, Julia R Köhler, Gary P Moran
Target of rapamycin complex 1 (TORC1) is an essential regulator of metabolism in eukaryotic cells and in the fungal pathogen Candida albicans regulates morphogenesis and nitrogen acquisition. Gtr1 encodes a highly conserved GTPase that in Saccharomyces cerevisiae regulates nitrogen sensing and TORC1 activation. Here, we characterize the role of C. albicans GTR1 in TORC1 activation and compare it with the previously characterized GTPase Rhb1. A homozygous gtr1/gtr1 mutant exhibited impaired TORC1-mediated phosphorylation of ribosomal protein S6 and increased susceptibility to rapamycin...
November 2017: MSphere
https://www.readbyqxmd.com/read/29146491/respiratory-stress-in-mitochondrial-electron-transport-chain-complex-mutants-of-candida-albicans-activates-snf1-kinase-response
#4
Pengyi Zhang, Hongbin Li, Jie Cheng, April Y Sun, Liqing Wang, Gordana Mircevska, Richard Calderone, Dongmei Li
We have previously established that mitochondrial Complex I (CI) mutants of Candida albicans display reduced oxygen consumption, decreased ATP production, and increased reactive oxidant species (ROS) during cell growth. Using the Seahorse XF96 analyzer, the energetic phenotypes of Electron Transport Chain (ETC) complex mutants are further characterized in the current study. The underlying regulation of energetic changes in these mutants is determined in glucose and non-glucose conditions when compared to wild type (WT) cells...
November 13, 2017: Fungal Genetics and Biology: FG & B
https://www.readbyqxmd.com/read/29139202/lego-inspired-drug-design-unveiling-of-the-novel-class-of-benzo-d-thiazole-containing-a-3-4-dihydroxyphenyl-moiety-as-plasma-membrane-h-atpase-inhibitors
#5
Truong Thanh Tung, Dao Trong Tuan, Marta Grifell Junyent, Michael Palmgren, Thomas Günther-Pomorski, Anja Thoe Fuglsang, Søren Brøgger Christensen, John Nielsen
The fungal plasma membrane H+-ATPase (Pma1p) is a potential target for the discovery of new antifungal agents. Surprisingly, no structure-activity relationship studies for small molecules targeting Pma1p have been reported. Herein, we disclose a LEGO-inspired fragment assembly strategy for design, synthesis and discovery of benzo[d]thiazoles containing a 3,4-dihydroxyphenyl moiety as potential Pma1p inhibitors. A series of 2-(benzo[d]thiazol-2-ylthio)-1-(3,4-dihydroxyphenyl)ethanones was found to inhibit Pma1p with the most potent IC50 of 8 µM in an in vitro plasma membrane H+-ATPase assay...
November 15, 2017: ChemMedChem
https://www.readbyqxmd.com/read/29129987/increasing-the-fungicidal-action-of-amphotericin-b-by-inhibiting-the-nitric-oxide-dependent-tolerance-pathway
#6
Kim Vriens, Phalguni Tewari Kumar, Caroline Struyfs, Tanne L Cools, Pieter Spincemaille, Tadej Kokalj, Belém Sampaio-Marques, Paula Ludovico, Jeroen Lammertyn, Bruno P A Cammue, Karin Thevissen
Amphotericin B (AmB) induces oxidative and nitrosative stresses, characterized by production of reactive oxygen and nitrogen species, in fungi. Yet, how these toxic species contribute to AmB-induced fungal cell death is unclear. We investigated the role of superoxide and nitric oxide radicals in AmB's fungicidal activity in Saccharomyces cerevisiae, using a digital microfluidic platform, which enabled monitoring individual cells at a spatiotemporal resolution, and plating assays. The nitric oxide synthase inhibitor L-NAME was used to interfere with nitric oxide radical production...
2017: Oxidative Medicine and Cellular Longevity
https://www.readbyqxmd.com/read/29119849/graphene-oxide-significantly-inhibits-cell-growth-at-sublethal-concentrations-by-causing-extracellular-iron-deficiency
#7
Qilin Yu, Bing Zhang, Jianrong Li, Tingting Du, Xiao Yi, Mingchun Li, Wei Chen, Pedro J J Alvarez
Graphene oxide (GO)-based materials are increasingly being used in medical materials and consumer products. However, their sublethal effects on biological systems are poorly understood. Here, we report that GO (at 10 to 160 mg/L) induced significant inhibitory effects on the growth of different unicellular organisms, including eukaryotes (i.e. Saccharomyces cerevisiae, Candida albicans, and Komagataella pastoris) and prokaryotes (Pseudomonas fluorescens). Growth inhibition could not be explained by commonly reported cytotoxicity mechanisms such as plasma membrane damage or oxidative stress...
November 9, 2017: Nanotoxicology
https://www.readbyqxmd.com/read/29101061/antimicrobial-efficacy-of-drug-blended-biosynthesized-colloidal-gold-nanoparticles-from-justicia-glauca-against-oral-pathogens-a-nanoantibiotic-approach
#8
Rayappan Emmanuel, Muthupandian Saravanan, Muhammad Ovais, Sethuramasamy Padmavathy, Zabta Khan Shinwari, Periyakaruppan Prakash
Antimicrobial resistance is a challenging task for researchers to develop new strategies. Green synthesis of AuNPs is an eco-friendly approach, which can be utilized in the microbistatic and microbicidal activities. The current study is focused on Justicia glauca (aqueous leaf extract) mediated AuNPs synthesis at room temperature by treating chloroaurate ions, that shows an antagonistic effect with Azithromycin (AZM) and Clarithromycin (CLR) antibiotics against oral pathogenic bacteria and fungi (Micrococcus luteus, Bacillus subtilis, Staphylococcus aureus, Streptococcus mutans, Lactobacillus acidophilus, Escherichia coli, Pseudomonas aeruginosa, Saccharomyces cerevisiae and Candida albicans)...
October 31, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/29069410/protein-kinase-c-in-fungi-more-than-just-cell-wall-integrity
#9
Jürgen J Heinisch, Rosaura Rodicio
Human protein kinase C (PKC) isoforms have been implicated in diseases such as Alzheimer's, diabetes, and cancers. In contrast to mammals, which have at least 9 genes, fungi have only one or two. The yeast Saccharomyces cerevisiae produces only a single Pkc1 and is employed in the study of specific human isozymes, including their susceptibility to pharmacological drugs. Vice versa , the domain structure and regulation of yeast and other fungal PKCs yield insights into the function of human isozymes. Therefore, human PKCs are briefly reviewed herein and related to the yeast enzyme...
October 23, 2017: FEMS Microbiology Reviews
https://www.readbyqxmd.com/read/28979764/saccharomyces-cerevisiae-show-low-levels-of-traversal-across-the-human-blood-brain-barrier-in-vitro
#10
Roberto Pérez-Torrado, Amparo Querol
BACKGROUND:   Saccharomyces cerevisiae is generally considered safe, and is involved in the production of many types of foods and dietary supplements. However, some isolates, which are genetically related to strains used in brewing and baking, have shown virulent traits, being able to produce infections in humans, mainly in immunodeficient patients. This can lead to systemic infections in humans. METHODS: In this work, we studied S. cerevisiae isolates in an in vitro human blood brain barrier model, comparing their behaviour with that of several strains of the related pathogens Candida glabrata and Candida albicans...
2017: F1000Research
https://www.readbyqxmd.com/read/28967545/the-n-terminal-py33xl-motif-of-capsy2-is-critical-for-the-function-of-protein-phosphatase-4-in-carad53-deactivation-dna-damage-induced-filamentation-and-virulence-in-candida-albicans
#11
Jinrong Feng, Yinong Duan, Yongwei Qin, Wei Sun, Zhong Zhuang, Dandan Zhu, Linghuo Jiang
Protein phosphatase PP4 is composed of one catalytic subunit and one or two regulatory subunits and conserved in eukaryotic cells. The catalytic subunit CaPph3 forms a complex with the regulatory subunit CaPsy2, which dephosphorylates activated CaRad53 during adaptation to and recovery from MMS-mediated DNA damage. We show here that the N-terminal Y33A mutation of CaPsy2 blocks the interaction between CaPph3 and CaRad53, the deactivation of CaRad53 and the morphologic switch in recovery from genotoxic stress...
September 22, 2017: International Journal of Medical Microbiology: IJMM
https://www.readbyqxmd.com/read/28955331/molecular-analysis-of-a-short-term-model-of-%C3%AE-glucans-trained-immunity-highlights-the-accessory-contribution-of-gm-csf-in-priming-mouse-macrophages-response
#12
Sarah Walachowski, Guillaume Tabouret, Marion Fabre, Gilles Foucras
β-Glucans (BGs) are glucose polymers present in the fungal cell wall (CW) and, as such, are recognized by innate immune cells as microbial-associated pattern through Dectin-1 receptor. Recent studies have highlighted the ability of the pathogenic yeast Candida albicans or its CW-derived β(1,3) (1,6)-glucans to increase human monocytes cytokine secretion upon secondary stimulation, a phenomenon now referred as immune training. This ability of monocytes programming confers BGs an undeniable immunotherapeutic potential...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28948565/experience-with-saccharomyces-boulardii-probiotic-in-oncohaematological-patients
#13
Beata Sulik-Tyszka, Emilian Snarski, Magda Niedźwiedzka, Małgorzata Augustyniak, Thorvald Nilsen Myhre, Anna Kacprzyk, Ewa Swoboda-Kopeć, Marta Roszkowska, Jadwiga Dwilewicz-Trojaczek, Wiesław Wiktor Jędrzejczak, Marta Wróblewska
Very few reports have been published to date on the bloodstream infections caused by Saccharomyces spp. in oncohaematological patients, and there are no guidelines on the use of this probiotic microorganism in this population. We describe the use of probiotic preparation containing Saccharomyces boulardii in a large group of oncohaematological patients. We retrospectively analysed the data from 32,000 patient hospitalisations at the haematological centre during 2011-2013 (including 196 haematopoietic stem cell transplant recipients) in a tertiary care university-affiliated hospital...
September 25, 2017: Probiotics and Antimicrobial Proteins
https://www.readbyqxmd.com/read/28942495/candida-albicans-fatty-acyl-coa-synthetase-cafaa4p-is-involved-in-the-uptake-of-exogenous-long-chain-fatty-acids-and-cell-activity-in-the-biofilm
#14
Kengo Tejima, Masanori Ishiai, Somay O Murayama, Shun Iwatani, Susumu Kajiwara
Fatty acyl-CoA synthetase (Faa) activates fatty acid (FA) by converting the FA into the CoA ester in the cell. In the present study, we characterized a FAA homologue (CaFAA4) from the opportunistic pathogen Candida albicans. Most organisms can not only synthesize long-chain fatty acyl-CoAs (LCFA-CoAs) endogenously using a fatty acid synthase (Fas) activity but also can uptake long-chain fatty acids (LCFAs) from the extracellular environment and convert them into LCFA-CoAs via a vectorial acylation system. The budding yeast Saccharomyces cerevisiae possesses two LCFA-CoA synthetases, ScFaa1p and ScFaa4p...
September 23, 2017: Current Genetics
https://www.readbyqxmd.com/read/28939893/characterization-of-the-antifungal-functions-of-a-wga-fc-igg2a-fusion-protein-binding-to-cell-wall-chitin-oligomers
#15
Susie Coutinho Liedke, Daniel Zamith Miranda, Kamilla Xavier Gomes, Jorge Luis S Gonçalves, Susana Frases, Joshua D Nosanchuk, Marcio L Rodrigues, Leonardo Nimrichter, José Mauro Peralta, Allan J Guimarães
The majority of therapeutic strategies for mycosis require the protracted administration of antifungals, which can result in significant toxicities and have unacceptable failure rates. Hence, there is an urgent need for the development of improved therapeutic approaches, and monoclonal antibody-based drugs are potentially a powerful alternative to standard antifungals. To develop a broad antibody-like reagent against mycosis, wheat germ agglutinin (WGA) was linked to the effector Fc region of murine IgG2a. The resultant WGA-Fc displayed high affinity to purified chitin and bound efficiently to fungal cell walls, co-localizing with chitin, in patterns ranging from circular (Histoplasma capsulatum) to punctate (Cryptococcus neoformans) to labeling at the bud sites (Candida albicans and Saccharomyces cerevisiae)...
September 22, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28919409/solasodine-3-o-%C3%AE-d-glucopyranoside-is-hydrolyzed-by-a-membrane-glucosidase-into-active-molecule-solasodine-against-candida-albicans
#16
Wenqiang Chang, Ying Li, Sha Zheng, Ming Zhang, Yanhui Gao, Hongxiang Lou
Antifungal activity of some natural molecules can be abated or blocked by efflux pumps in Candida albicans, which restricts the discovery of potential antifungal agents. Here we found that the steroidal alkaloid solasodine is active against C. albicans efflux pump-deficient strains but inert towards the wild type. However, the glucosylated solasodine-3-O-β-d-glucopyranoside exhibits antifungal activity towards the wild type strain. Further investigation revealed that the entry of solasodine into C. albicans cells is blocked by efflux pumps...
September 15, 2017: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/28893786/novel-cell-killing-mechanisms-of-hydroxyurea-and-the-implication-toward-combination-therapy-for-the-treatment-of-fungal-infections
#17
Amanpreet Singh, Ameeta Agarwal, Yong-Jie Xu
We have previously reported that an erg11 mutation affecting ergosterol synthesis and a hem13 mutation in the heme synthesis pathway significantly sensitize the fission yeast Schizosaccharomyces pombe to hydroxyurea (HU) (1, 2). Here we show that treatment with inhibitors of Erg11 and heme biosynthesis phenocopies the two mutations in sensitizing wild-type cells to HU. Importantly, HU synergistically interacts with the heme biosynthesis inhibitor sampangine and several Erg11 inhibitors, the antifungal azoles, in causing cell lethality...
November 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28852966/characterization-of-the-fungal-flora-of-dolo-a-traditional-fermented-beverage-of-burkina-faso-using-maldi-tof-mass-spectrometry
#18
Bamba Sanata, Zida Adama, Sangaré Ibrahim, Sondo Apoline, Cissé Mamoudou, Sirima Constant, Guiguemdé T Robert, Hennequin Christophe
A cross-sectional study was conducted in Bobo-Dioulasso, Burkina Faso, to identify the yeast diversity associated with the manufacture of dolo, a traditional fermented beverage of Burkina Faso. From sixty specimens spread onto chromogenic medium plates, sixty-two strains were isolated then identified using MALDI-TOF analysis. Seven yeast species were identified, Saccharomyces cerevisiae (39%) followed by Pichia manshurica (18%) being the most frequent. Forty-three percent of the samples contained Candida species, notably Candida albicans...
August 29, 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28829379/phosphate-acquisition-and-virulence-in-human-fungal-pathogens
#19
REVIEW
Mélanie Ikeh, Yasmin Ahmed, Janet Quinn
The ability of pathogenic fungi to acquire essential macro and micronutrients during infection is a well-established virulence trait. Recent studies in the major human fungal pathogens Candida albicans and Cryptococcus neoformans have revealed that acquisition of the essential macronutrient, phosphate, is essential for virulence. The phosphate sensing and acquisition pathway in fungi, known as the PHO pathway, has been extensively characterized in the model yeast Saccharomyces cerevisiae. In this review, we highlight recent advances in phosphate sensing and signaling mechanisms, and use the S...
August 22, 2017: Microorganisms
https://www.readbyqxmd.com/read/28810704/yeast-kch1-and-kch2-membrane-proteins-play-a-pleiotropic-role-in-membrane-potential-establishment-and-monovalent-cation-homeostasis-regulation
#20
Kristina Felcmanova, Petra Neveceralova, Hana Sychrova, Olga Zimmermannova
The Kch1 and Kch2 plasma-membrane proteins were identified in Saccharomyces cerevisiae as being essential for the activation of a high-affinity Ca2+ influx system. We searched for Kch proteins roles in the maintenance of cation homeostasis and tested the effect of kch1 and/or kch2 deletions on various physiological parameters. Compared to wild-type, kch1 kch2 mutant cells were smaller, relatively hyperpolarised, grew better under limited K+ conditions and exhibited altered growth in the presence of monovalent cations...
August 1, 2017: FEMS Yeast Research
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