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Beauveria bassiana

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https://www.readbyqxmd.com/read/28208575/rna-seq-analyses-for-two-silkworm-strains-reveals-insight-into-their-susceptibility-and-resistance-to-beauveria-bassiana-infection
#1
Dongxu Xing, Qiong Yang, Liang Jiang, Qingrong Li, Yang Xiao, Mingqiang Ye, Qingyou Xia
The silkworm Bombyx mori is an economically important species. White muscardine caused by Beauveria bassiana is the main fungal disease in sericulture, and understanding the silkworm responses to B. bassiana infection is of particular interest. Herein, we investigated the molecular mechanisms underlying these responses in two silkworm strains Haoyue (HY, sensitive to B. bassiana) and Kang 8 (K8, resistant to B. bassiana) using an RNA-seq approach. For each strain, three biological replicates for immersion treatment, two replicates for injection treatment and three untreated controls were collected to generate 16 libraries for sequencing...
February 10, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28194425/molecular-cloning-bioinformatic-analysis-and-expression-of-bombyx-mori-lebocin-5-gene-related-to-beauveria-bassiana-infection
#2
Dingding Lü, Chengxiang Hou, Guangxing Qin, Kun Gao, Tian Chen, Xijie Guo
A full-length cDNA of lebocin 5 (BmLeb5) was first cloned from silkworm, Bombyx mori, by rapid amplification of cDNA ends. The BmLeb5 gene is 808 bp in length and the open reading frame encodes a 179-amino acid hydroxyproline-rich peptide. Bioinformatic analysis results showed that BmLeb5 owns an O-glycosylation site and four RXXR motifs as other lebocins. Sequence similarity and phylogenic analysis results indicated that lebocins form a multiple gene family in silkworm as cecropins. Quantitative real-time PCR analysis revealed that BmLeb5 was highest expressed in the fat body...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28193896/regulatory-cascade-and-biological-activity-of-beauveria-bassiana-oosporein-that-limits-bacterial-growth-after-host-death
#3
Yanhua Fan, Xi Liu, Nemat O Keyhani, Guirong Tang, Yan Pei, Wenwen Zhang, Sheng Tong
The regulatory network and biological functions of the fungal secondary metabolite oosporein have remained obscure. Beauveria bassiana has evolved the ability to parasitize insects and outcompete microbial challengers for assimilation of host nutrients. A novel zinc finger transcription factor, BbSmr1 (B. bassiana secondary metabolite regulator 1), was identified in a screen for oosporein overproduction. Deletion of Bbsmr1 resulted in up-regulation of the oosporein biosynthetic gene cluster (OpS genes) and constitutive oosporein production...
February 13, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28168316/additive-roles-of-two-tps-genes-in-trehalose-synthesis-conidiation-multiple-stress-responses-and-host-infection-of-a-fungal-insect-pathogen
#4
Juan-Juan Wang, Qing Cai, Lei Qiu, Sheng-Hua Ying, Ming-Guang Feng
Intracellular trehalose accumulation is relevant to fungal life and pathogenicity. Trehalose-6-phosphate synthase (TPS) is known to control the first step of trehalose synthesis, but functions of multiple TPS genes in some filamentous fungi are variable. Here, we examined the functions of two TPS genes (tpsA and tpsB) in Beauveria bassiana, a fungal insect pathogen widely applied in arthropod pest control. Intracellular TPS activity and trehalose content decreased by 71-75 and 72-80% in ΔtpsA, and 21-30 and 15-45% in ΔtpsB, respectively, and to undetectable levels in ΔtpsAΔtpsB, under normal and stressful conditions...
February 6, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28114361/studies-on-the-virome-of-the-entomopathogenic-fungus-beauveria-bassiana-reveal-novel-dsrna-elements-and-mild-hypervirulence
#5
Ioly Kotta-Loizou, Robert H A Coutts
The entomopathogenic fungus Beauveria bassiana has a wide host range and is used as a biocontrol agent against arthropod pests. Mycoviruses have been described in phytopathogenic fungi while in entomopathogenic fungi their presence has been reported only rarely. Here we show that 21.3% of a collection of B. bassiana isolates sourced from worldwide locations, harbor dsRNA elements. Molecular characterization of these elements revealed the prevalence of mycoviruses belonging to the Partitiviridae and Totiviridae families, the smallest reported virus to date, belonging to the family Narnaviridae, and viruses unassigned to a family or genus...
January 2017: PLoS Pathogens
https://www.readbyqxmd.com/read/28108175/characterization-of-the-synergistic-interaction-between-beauveria-bassiana-strain-gha-and-bacillus-thuringiensis-morrisoni-strain-tenebrionis-applied-against-colorado-potato-beetle-larvae
#6
S P Wraight, M E Ramos
Studies were undertaken to further characterize the previously identified synergistic activity of Bacillus thuringiensis- and Beauveria bassiana-based biopesticides against Colorado potato beetle (CPB). A flowable concentrate of B. thuringiensis morrisoni strain tenebrionis (Bt) (Novodor® FC) and a wettable powder of B. bassiana strain GHA (Bb) (Mycotrol® 22WP) were applied against CPB larval populations infesting potato in field plots. Novodor FC and an oil-dispersion formulation of Bb (Mycotrol ES) were applied against second-instar CPB larvae on potted potato plants in greenhouse tests under low relative humidity (RH), variable-temperature conditions...
January 17, 2017: Journal of Invertebrate Pathology
https://www.readbyqxmd.com/read/28094453/down-regulation-of-a-chitin-synthase-a-gene-by-rna-interference-enhances-pathogenicity-of-beauveria-bassiana-anu1-against-spodoptera-exigua-h%C3%A3-bner
#7
Jung-Bok Lee, Hyun Soo Kim, Youngjin Park
Chitin synthase (CHS) is an important enzymatic component, which is required for chitin formation in the cuticles and cuticular linings of other tissues in insects. CHSs have been divided into two classes, classes A and B, based on their amino acid sequence similarities and functions. Class A CHS (CHS-A) is specifically expressed in the epidermis and related ectodermal cells such as tracheal cells, while class B CHS (CHS-B) is expressed in gut epithelial cells that produce peritrophic matrices. In this study, we cloned the CHS-A gene from the beet armyworm, Spodoptera exigua (SeCHS-A)...
January 17, 2017: Archives of Insect Biochemistry and Physiology
https://www.readbyqxmd.com/read/28089049/variation-in-virulence-of-beauveria-bassiana-and-b-pseudobassiana-to-the-pine-weevil-pissodes-nemorensis-in-relation-to-mycelium-characteristics-and-virulence-genes
#8
Pedro Romón, Hardus Hatting, Arturo Goldarazena, Juan Carlos Iturrondobeitia
Entomopathogenic fungi such as Beauveria spp. have potential applications in the biocontrol of insect pests but little is known regarding their infectivity to the pine weevil Pissodes nemorensis. In this study, five isolates of Beauveria pseudobassiana and five isolates of Beauveria bassiana were tested for characteristics correlating with virulence on P. nemorensis. Isolate UAMH301 had the lowest mean lethal concentration value whereas the highest value was obtained with isolate LRC137. Growth rate was negatively correlated with virulence in B...
February 2017: Fungal Biology
https://www.readbyqxmd.com/read/28076898/characterization-of-the-hog1-mapk-pathway-in-the-entomopathogenic-fungus-beauveria-bassiana
#9
Jing Liu, Zhi-Kang Wang, Huan-Huan Sun, Sheng-Hua Ying, Ming-Guang Feng
High-osmolarity glycerol (HOG) pathway required for yeast osmoregulation relies upon the mitogen-activated protein kinase (MAPK) Hog1 cascade that comprise the MAPKKKs Ssk2/Ssk22 and Ste11 converging on the MAPKK Pbs2. Here we show a Hog1 cascade with the unique MAPKKK Ssk2 acting in Beauveria bassiana. Hypersensitivity to high osmolarity and high resistance to fludioxonil fungicide appeared in Δssk2, Δpbs2 and Δhog1 mutants whereas the two hallmark phenotypes were reversed in Δste11. Increased sensitivity to heat shock and decreased sensitivity to cell wall perturbation also occurred in the three mutants but not in Δste11 although antioxidant phenotypes were different in all deletion mutants...
January 11, 2017: Environmental Microbiology
https://www.readbyqxmd.com/read/28066976/delivering-beauveria-bassiana-with-electrostatic-powder-for-the-control-of-stored-product-beetles
#10
Athanassiou G Christos, Rumbos I Christos, Sakka Maria, Storm Clare, Dillon Aoife, Potin Olivier
BACKGROUND: The efficacy of a Beauveria bassiana-based formulation (Bb38) with Entostat, an electrostatically charged powder, was investigated as surface treatment against Cryptolestes ferrugineus, Oryzaephilus surinamensis and Sitophilus granarius adults. In lab bioassays, the efficacy of Bb38 against the aforementioned species was examined on concrete, plywood, steel and ceramic, whereas its residual efficacy against the same species was assessed on concrete and steel in the presence or absence of illumination...
January 9, 2017: Pest Management Science
https://www.readbyqxmd.com/read/28054950/microbial-glycosylation-of-daidzein-genistein-and-biochanin-a-two-new-glucosides-of-biochanin-a
#11
Sandra Sordon, Jarosław Popłoński, Tomasz Tronina, Ewa Huszcza
Biotransformation of daidzein, genistein and biochanin A by three selected filamentous fungi was investigated. As a result of biotransformations, six glycosylation products were obtained. Fungus Beauveria bassiana converted all tested isoflavones to 4″-O-methyl-7-O-glucosyl derivatives, whereas Absidia coerulea and Absidia glauca were able to transform genistein and biochanin A to genistin and sissotrin, respectively. In the culture of Absidia coerulea, in addition to the sissotrin, the product of glucosylation at position 5 was formed...
January 3, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28040530/correlation-of-cell-surface-proteins-of-distinct-beauveria-bassiana-cell-types-and-adaption-to-varied-environment-and-interaction-with-the-host-insect-cell-surface-associated-proteins-of-b-bassiana
#12
Zhi Yang, Hongyan Jiang, Xin Zhao, Zhuoyue Lu, Zhibing Luo, Xuebing Li, Jing Zhao, Yongjun Zhang
The insect fungal pathogen Beauveria bassiana produces a number of distinct cell types that include aerial conidia, blastospores and haemolymph-derived cells, termed hyphal bodies, to adapt varied environment niches and within the host insect. These cells display distinct biochemical properties and surface structures, and a highly ordered outermost brush-like structure uniquely present on hyphal bodies, but not on any in vitro cells. Here, we found that the outermost structure on the hyphal bodies mainly consisted of proteins associated to structural wall components in that most of it could be removed by dithiothreitol (DTT) or proteinase K...
December 28, 2016: Fungal Genetics and Biology: FG & B
https://www.readbyqxmd.com/read/28032511/isolation-and-characterization-of-2-pyridone-alkaloids-and-alloxazines-from-beauveria-bassiana
#13
W J Andrioli, A A Lopes, B C Cavalcanti, C Pessoa, N P D Nanayakkara, J K Bastos
Two novel compounds bearing heterocyclic nitrogen, 2-pyridone alkaloid (1) and alloxazine derivative (2), along with the known pretenellin B (3), pyridovericin (4) and lumichrome (5) were isolated from a culture of the entomopathogenic fungal strain Beauveria bassiana. The chemical structures of 2-pyridone alkaloid and alloxazine derivative were established on the basis of the interpretation of spectroscopic data. The isolated compounds were evaluated in a panel of five cancer cell lines and pyridovericin exhibited cytotoxicity (IC50, μM) against cancer cell lines: HL-60 (25...
December 29, 2016: Natural Product Research
https://www.readbyqxmd.com/read/28011319/effect-of-vacuolar-atpase-subunit-h-vmah-on-cellular-ph-asexual-cycle-stress-tolerance-and-virulence-in-beauveria-bassiana
#14
Jing Zhu, Xiao-Guan Zhu, Sheng-Hua Ying, Ming-Guang Feng
Vacuolar ATPase (V-ATPase) is a conserved multi-subunit protein complex that mediates intracellular acidification in fungi. Here we show functional diversity of V-ATPase subunit H (BbVmaH) in Beauveria bassiana, a filamentous fungal insect pathogen. Deletion of BbvmaH resulted in elevated vacuolar pH, increased Ca(2+) level in cytosol but not in vacuoles, accelerated culture acidification and reduced accumulation of extracellular ammonia. Aerial conidiation and submerged blastospore production were largely delayed and reduced in the deletion mutant, respectively, accompanied with a significant delay in conidial germination, alterations of conidia and blastospores in morphology, size and/or density, and severe growth defects in minimal media with different carbon and nitrogen sources...
December 21, 2016: Fungal Genetics and Biology: FG & B
https://www.readbyqxmd.com/read/27995045/in-vitro-interaction-of-metarhizium-anisopliae-ma9236-and-beauveria-bassiana-bb9205-with-heterorhabditis-bacteriophora-hni0100-for-the-control-of-plutella-xylostella
#15
J P Correa-Cuadros, A Sáenz-Aponte, M X Rodríguez-Bocanegra
The diamondback moth (Plutella xylostella) is a major pest of broccoli crops in Colombia. To control P. xylostella, we evaluated the interaction of Beauveria bassiana Bb9205 and Metarhizium anisopliae Ma9236 with Heterorhabditis bacteriophora HNI0100 and its bacterial symbiont Photorhabdus luminescens HNI0100. We used antagonism and disk diffusion assays with fungal extracts to test the interaction between symbiotic bacterium and fungi. P. luminescens inhibited the growth of B. bassiana and M. anisopliae up to 40% by the secretion of secondary metabolites, whereas fungal extracts did not inhibit P...
2016: SpringerPlus
https://www.readbyqxmd.com/read/27986935/bioassay-and-scanning-electron-microscopic-observations-reveal-high-virulence-of-entomopathogenic-fungus-beauveria-bassiana-on-the-onion-maggot-diptera-anthomyiidae-adults
#16
Hui Zhang, Shengyong Wu, Zhenlong Xing, Xiaoqing Wang, Zhongren Lei
When flies were dipped in 1 × 10(8) conidia/ml conidia suspensions and then kept in the incubator (22 ± 1 °C, 70 ± 5% RH), scanning electron microscope observations revealed that, at 2 h, the majority of adhering Beauveria bassiana conidia were attached to either the wing surface or the interstitial area between the macrochaetae on the thorax and abdomen of the onion maggot adults. Germ tubes were being produced and had oriented toward the cuticle by 18 h. Penetration of the insect cuticle had occurred by 36 h, and by 48 h, germ tubes had completely penetrated the cuticle...
December 2016: Journal of Economic Entomology
https://www.readbyqxmd.com/read/27933578/inhibitory-activity-of-beauveria-bassiana-and-trichoderma-spp-on-the-insect-pests-xylotrechus-arvicola-coleoptera-cerambycidae-and-acanthoscelides-obtectus-coleoptera-chrisomelidae-bruchinae
#17
Álvaro Rodríguez-González, Sara Mayo, Óscar González-López, Bonifacio Reinoso, Santiago Gutierrez, Pedro Antonio Casquero
Xylotrechus arvicola is an important pest in vineyards (Vitis vinifera) in the main Iberian wine-producing regions, and Acanthoscelides obtectus causes severe post-harvest losses in the common bean (Phaseolus vulgaris). Under laboratory conditions with a spray tower, the susceptibility of the immature stages of X. arvicola and A. obtectus against the entomopathogenic fungi Beauveria bassiana and four strains of Trichoderma spp. was evaluated. Both insect pests T. harzianum and B. bassiana showed a good inhibitory activity, accumulating an inhibition on the eggs of values above 85 and 82%, respectively...
January 2017: Environmental Monitoring and Assessment
https://www.readbyqxmd.com/read/27914926/metschnikowia-cf-typographi-and-other-pathogens-from-the-bark-beetle-ips-sexdentatus-prevalence-histological-and-ultrastructural-evidence-and-molecular-characterization
#18
Regina G Kleespies, Young Woon Lim, Cezary Tkaczuk, Marta Wrzosek, Bernhardt M Steinwender, Rudolf Wegensteiner
Ips sexdentatus (six-spined engraver beetle) from Austria and Poland were dissected and examined for the presence of pathogens. Specimens collected in Austria were found to contain the ascomycetous fungus Metschnikowia cf. typographi. Infection rates ranged from 3.6% to 26.8% at different collection sites. M. cf. typographi infected midguts were investigated by histological, ultrastructural and molecular techniques. Extraordinary ultrastructural details are shown, such as ascospores with bilateral flattened flanks resembling alar rims at both sides of their attenuating tube-like ends...
December 1, 2016: Journal of Invertebrate Pathology
https://www.readbyqxmd.com/read/27905873/comparative-genomics-of-beauveria-bassiana-uncovering-signatures-of-virulence-against-mosquitoes
#19
Claudio A Valero-Jiménez, Luigi Faino, Daphne Spring In't Veld, Sandra Smit, Bas J Zwaan, Jan A L van Kan
BACKGROUND: Entomopathogenic fungi such as Beauveria bassiana are promising biological agents for control of malaria mosquitoes. Indeed, infection with B. bassiana reduces the lifespan of mosquitoes in the laboratory and in the field. Natural isolates of B. bassiana show up to 10-fold differences in virulence between the most and the least virulent isolate. In this study, we sequenced the genomes of five isolates representing the extremes of low/high virulence and three RNA libraries, and applied a genome comparison approach to uncover genetic mechanisms underpinning virulence...
December 1, 2016: BMC Genomics
https://www.readbyqxmd.com/read/27889557/identification-of-a-hemolin-protein-from-actias-selene-mediates-immune-response-to-pathogens
#20
Cen Qian, Fang Wang, Baojian Zhu, Lei Wang, Guoqing Wei, Yu Sun, Sheng Li, Chaoliang Liu
Hemolins play an important role in development and innate immunity in insects. In this study, a hemolin cDNA of 1412bp in Actias selene (As-HEM) was isolated and its open reading frames (ORFs) were 420 amino acid residues. Sequence analysis indicated As-HEM was homologous to those hemolins from other insects species. The recombinant protein of As-HEM was expressed in Escherichia coli, and anti-As-HEM antibodies were prepared. Real-time quantitative PCR (RT-qPCR) and western blot results revealed that mRNA and protein levels of As-HEM were mostly detected in hemocytes and hemolymph...
January 2017: International Immunopharmacology
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