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https://www.readbyqxmd.com/read/29775929/negative-impacts-of-elevated-nitrate-on-physiological-performance-are-not-exacerbated-by-low-ph
#1
Daniel F Gomez Isaza, Rebecca L Cramp, Craig E Franklin
Multiple environmental stressors, including nutrient effluents (i.e. nitrates [NO3 - ]) and altered pH regimes, influence the persistence of freshwater species in anthropogenically disturbed habitats. Independently, nitrate and low pH affect energy allocation by increasing maintenance costs and disrupting oxygen uptake, which ultimately results in impacts upon whole animal performance. However, the interaction between these two stressors has not been characterised. To address this, the effects of nitrate and pH and their interaction on aerobic scope and physiological performance were investigated in the blueclaw crayfish, Cherax destructor...
May 15, 2018: Aquatic Toxicology
https://www.readbyqxmd.com/read/29775421/probability-of-heat-intolerance-standardized-interpretation-of-heat-tolerance-testing-results-versus-specialist-judgment
#2
Haggai Schermann, Erin Craig, Einat Yanovich, Itay Ketko, Gary Kalmanovich, Ran Yanovich
CONTEXT:   The heat-tolerance test (HTT) is a screening tool for secondary prevention of exertional heat illness by the Israel Defense Forces. To discern participant tolerance, recruits are exposed to intermediate environmental and exercise stresses, and their physiological responses, core temperature, and heart rate are monitored. When their physiological measures rise at a higher rate or exceed the upper levels of absolute values compared with other participants, heat intolerance (HI) is diagnosed...
April 2018: Journal of Athletic Training
https://www.readbyqxmd.com/read/29775401/electroencephalographic-signals-during-anesthesia-recorded-from-surface-and-depth-electrodes
#3
Ville Jäntti, Tuomo Ylinen, Narayan Puthanmadam Subramaniyam, Kotoe Kamata, Arvi Yli-Hankala, Pasi Kauppinen, Eila Sonkajärvi
Purpose - Anesthesiologists have increasingly started to use EEG based indexes to estimate the level and type of unconsciousness. However, the physiology and biophysics are poorly understood in anesthesiological literature. Methods - EEG was recorded from electrodes on the surface of head, including scalp, as well as DBS (deep brain stimulation) electrodes implanted deep in the brain. Mathematical modeling with a realistic head model was performed to create illustrative images of the sensitivity of electrode montages...
May 18, 2018: International Journal of Radiation Biology
https://www.readbyqxmd.com/read/29775080/mindfulness-meditation-and-interprofessional-cardiopulmonary-resuscitation-a-mixed-methods-pilot-study
#4
Diana J Kelm, Jennifer L Ridgeway, Becca L Gas, Monali Mohan, David A Cook, Darlene R Nelson, Roberto P Benzo
PROBLEM: Mindfulness training includes mindfulness meditation, which has been shown to improve both attention and self-awareness. Medical providers in the intensive care unit often deal with difficult situations with strong emotions, life-and-death decisions, and both interpersonal and interprofessional conflicts. The effect of mindfulness meditation training on healthcare providers during acute care tasks such as cardiopulmonary resuscitation remains unknown. Mindfulness meditation has the potential to improve provider well-being and reduce stress in individuals involved in resuscitation teams, which could then translate into better team communication and delivery of care under stress...
May 18, 2018: Teaching and Learning in Medicine
https://www.readbyqxmd.com/read/29774921/a-flexible-pressure-sensor-based-on-rgo-polyaniline-wrapped-sponge-with-tunable-sensitivity-for-human-motion-detection
#5
Gang Ge, Yichen Cai, Qiuchun Dong, Yizhou Zhang, Jinjun Shao, Wei Huang, Xiaochen Dong
High-performance stretchable and wearable electronic skins (E-skins) with high sensitivity and a large sensing range are urgently required with the rapid development of the Internet of things and artificial intelligence. Herein, a reduced graphene oxide (rGO)/polyaniline wrapped sponge is prepared via rGO coating and the in situ synthesis of polyaniline nanowires (PANI NWs) on the backbones of sponge for the fabrication of pressure sensors. From the as-prepared flexible sensor, tunable sensitivity (0.042 to 0...
May 18, 2018: Nanoscale
https://www.readbyqxmd.com/read/29773977/journey-to-the-center-of-the-fetal-brain-environmental-exposures-and-autophagy
#6
REVIEW
Jun Lei, Pilar Calvo, Richard Vigh, Irina Burd
Fetal brain development is known to be affected by adverse environmental exposures during pregnancy, including infection, inflammation, hypoxia, alcohol, starvation, and toxins. These exposures are thought to alter autophagy activity in the fetal brain, leading to adverse perinatal outcomes, such as cognitive and sensorimotor deficits. This review introduces the physiologic autophagy pathways in the fetal brain. Next, methods to detect and monitor fetal brain autophagy activity are outlined. An additional discussion explores possible mechanisms by which environmental exposures during pregnancy alter fetal brain autophagy activity...
2018: Frontiers in Cellular Neuroscience
https://www.readbyqxmd.com/read/29772475/impact-of-cu-ii-doping-on-the-vulnerability-of-escherichia-coli-atcc-10536-revealed-by-atomic-force-microscopy
#7
Wasia Rasheed, Samina Perveen, Ghulam Mustafa, Muhammad Raza Shah, Shakil Ahmed, Sami Uzzaman
E. coli strain is a gram-negative bacterium known to induce both extra-intestinal infections and intestinal infections. For survival of microbes, metal intake and accessibility should be according to their physiological requirements. Peculiarly, copper homeostasis is critical for E. coli survival and growth. Therefore in this study, an extensive work is conducted to investigate the impact of Cu(II)-doping on the susceptibility of Escherichia coli ATCC 10536 against Cu(II)-selective Cefaclor-silver nanoconjugates (i...
May 8, 2018: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/29770889/autoregulation-in-the-neuro-icu
#8
REVIEW
Anson Wang, Santiago Ortega-Gutierrez, Nils H Petersen
PURPOSE OF REVIEW: The purpose of this review is to briefly describe the concept of cerebral autoregulation, to detail several bedside techniques for measuring and assessing autoregulation, and to outline the impact of impaired autoregulation on clinical and functional outcomes in acute brain injury. Furthermore, we will review several autoregulation studies in select forms of acute brain injuries, discuss the potential for its use in patient management in the ICU, and suggest further avenues for research...
May 17, 2018: Current Treatment Options in Neurology
https://www.readbyqxmd.com/read/29770421/quantitative-proteomics-and-systems-analysis-of-cultured-h9c2-cardiomyoblasts-during-differentiation-over-time-supports-a-function-follows-form-model-of-differentiation
#9
Cynthia Kankeu, Kylie Clarke, Delphi Van Haver, Kris Gevaert, Francis Impens, Anna Dittrich, H Llewelyn Roderick, Egle Passante, Heinrich J Huber
The rat cardiomyoblast cell line H9C2 has emerged as a valuable tool for studying cardiac development, mechanisms of disease and toxicology. We present here a rigorous proteomic analysis that monitored the changes in protein expression during differentiation of H9C2 cells into cardiomyocyte-like cells over time. Quantitative mass spectrometry followed by gene ontology (GO) enrichment analysis revealed that early changes in H9C2 differentiation are related to protein pathways of cardiac muscle morphogenesis and sphingolipid synthesis...
May 17, 2018: Molecular omics
https://www.readbyqxmd.com/read/29770207/recent-advances-in-bioreactors-for-cell-based-therapies
#10
REVIEW
Makeda Stephenson, Warren Grayson
Bioreactors have become indispensable tools in the cell-based therapy industry. Various forms of bioreactors are used to maintain well-controlled microenvironments to regulate cell growth, differentiation, and tissue development. They are essential for providing standardized, reproducible cell-based products for regenerative medicine applications or to establish physiologically relevant in vitro models for testing of pharmacologic agents. In this review, we discuss three main classes of bioreactors: cell expansion bioreactors, tissue engineering bioreactors, and lab-on-a-chip systems...
2018: F1000Research
https://www.readbyqxmd.com/read/29768943/early-reduced-behavioral-activity-induced-by-large-strokes-affects-the-efficiency-of-enriched-environment-in-rats
#11
Anna-Sophia Wahl, Eva Erlebach, Biagio Brattoli, Uta Büchler, Julia Kaiser, Benjamin V Ineichen, Alice C Mosberger, Shirin Schneeberger, Stefan Imobersteg, Martin Wieckhorst, Martina Stirn, Aileen Schroeter, Bjoern Ommer, Martin E Schwab
The majority of stroke patients develop post-stroke fatigue, a symptom which impairs motivation and diminishes the success of rehabilitative interventions. We show that large cortical strokes acutely reduce activity levels in rats for 1-2 weeks as a physiological response paralleled by signs of systemic inflammation. Rats were exposed early (1-2 weeks) or late (3-4 weeks after stroke) to an individually monitored enriched environment to stimulate self-controlled high-intensity sensorimotor training. A group of animals received Anti-Nogo antibodies for the first two weeks after stroke, a neuronal growth promoting immunotherapy already in clinical trials...
January 1, 2018: Journal of Cerebral Blood Flow and Metabolism
https://www.readbyqxmd.com/read/29767806/telephone-support-and-telemonitoring-for-low-income-older-adults
#12
Suyong Jeong, Hanna Choi, Seok Hyun Gwon, Jeongeun Kim
The objective of the current pilot study was to determine whether nurse-led telephone counseling improves health behavior, self-care, and physiological indices for low-income older adults using a telemonitoring system. The control group (n = 15) was provided with weekly health education only, and the intervention group (n = 20) was given additional telephone support by nurses. At baseline and 8 weeks, data on health and self-care behaviors were collected using a self-reported questionnaire, and blood pressure and fasting blood glucose levels were assessed...
May 16, 2018: Research in Gerontological Nursing
https://www.readbyqxmd.com/read/29767569/teaching-tip-development-of-veterinary-anesthesia-simulations-for-pre-clinical-training-design-implementation-and-evaluation-based-on-student-perspectives
#13
Jana L Jones, Jim Rinehart, Jacqueline Jordan Spiegel, Ryane E Englar, Brian K Sidaway, Joie Rowles
Anesthesia simulations have been used in pre-clinical medical training for decades to help learners gain confidence and expertise in an operating room environment without danger to a live patient. The authors describe a veterinary anesthesia simulation environment (VASE) with anesthesia scenarios developed to provide a re-creation of a veterinarian's task environment while performing anesthesia. The VASE uses advanced computer technology with simulator inputs provided from standard monitoring equipment in common use during veterinary anesthesia and a commercial canine training mannequin that allows intubation, ventilation, and venous access...
2018: Journal of Veterinary Medical Education
https://www.readbyqxmd.com/read/29765381/sequence-of-changes-in-maize-responding-to-soil-water-deficit-and-related-critical-thresholds
#14
Xueyan Ma, Qijin He, Guangsheng Zhou
The sequence of changes in crop responding to soil water deficit and related critical thresholds are essential for better drought damage classification and drought monitoring indicators. This study was aimed to investigate the critical thresholds of maize growth and physiological characteristics responding to changing soil water and to reveal the sequence of changes in maize responding to soil water deficit both in seedling and jointing stages based on 2-year's maize field experiment responding to six initial soil water statuses conducted in 2013 and 2014...
2018: Frontiers in Plant Science
https://www.readbyqxmd.com/read/29765362/tachyplesin-causes-membrane-instability-that-kills-multidrug-resistant-bacteria-by-inhibiting-the-3-ketoacyl-carrier-protein-reductase-fabg
#15
Cunbao Liu, Jialong Qi, Bin Shan, Yanbing Ma
Tachyplesin is a type of cationic β-hairpin antimicrobial peptide discovered in horseshoe crab approximately 30 years ago that is well known for both its potential antimicrobial activities against multidrug-resistant bacteria and its cytotoxicity to mammalian cells. Though its physical interactions with artificial membranes have been well studied, details of its physiological mechanism of action the physiological consequences of its action remain limited. By using the DNA-binding fluorescent dye propidium iodide to monitor membrane integrity, confocal microscopy to assess the intracellular location of FITC-tagged tachyplesin, and RNA sequencing of the differentially expressed genes in four Gram-negative bacteria ( Escherichia coli, Acinetobacter baumannii, Klebsiella pneumoniae , and Pseudomonas aeruginosa ) treated with lethal or sublethal concentrations of tachyplesin, we found that compared with levofloxacin-treated bacteria, tachyplesin-treated bacteria showed significant effects on the pathways underlying unsaturated fatty acid biosynthesis...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29764915/remote-physiological-monitoring-in-an-austere-environment-a-future-for-battlefield-care-provision
#16
Matthew J Smyth, J A Round, A J Mellor
Wearable technologies are making considerable advances into the mainstream as they become smaller and more user friendly. The global market for such devices is forecasted to be worth over US$5 billion in 2018, with one in six people owning a device. Many professional sporting teams use self-monitoring to assess physiological parameters and work rate on the pitch, highlighting the potential utility for military command chains. As size of device reduces and sensitivity improves, coupled with remote connectivity technology, integration into the military environment could be relatively seamless...
May 14, 2018: Journal of the Royal Army Medical Corps
https://www.readbyqxmd.com/read/29764550/-predictive-value-of-central-venous-to-arterial-carbon-dioxide-partial-pressure-difference-for-fluid-responsiveness-in-septic-shock-patients-a-prospective-clinical-study
#17
Guangyun Liu, Huibin Huang, Hanyu Qin, Bin Du
OBJECTIVE: To evaluate the accuracy of central venous-to-arterial carbon dioxide partial pressure difference (Pcv-aCO2 ) before and after rapid rehydration test (fluid challenge) in predicting the fluid responsiveness in patients with septic shock. METHODS: A prospective observation was conducted. Forty septic shock patients admitted to medical intensive care unit (ICU) of Peking Union Medical College Hospital from October 2015 to June 2017 were enrolled. All of the patients received fluid challenge in the presence of invasive hemodynamic monitoring...
May 2018: Zhonghua Wei Zhong Bing Ji Jiu Yi Xue
https://www.readbyqxmd.com/read/29761964/-real-time-investigation-of-dynamic-morphology-of-live-platelets-and-generation-of-platelet-microparticles-using-hopping-probe-ion-conductance-microscopy
#18
Xiao Liu, Yufu Luo, Yanjun Zhang
Platelets are rapidly activated by activators and produce a large number of platelet microparticles (PMPs) with high coagulation activity, resulting in coagulation dysfunction. However, the generation mechanism of PMPs is still not clear. Hopping probe ion conductance microscopy (HPICM) has special technical advantages in non-contact, real-time, high-resolution imaging of living cells under physiological conditions. Using HPICM, this study monitored the processes of platelet activation and generation of PMPs in real time in the presence of calcium ionophore A23187 and cytochalasin D (CD), respectively...
August 1, 2017: Sheng Wu Yi Xue Gong Cheng Xue za Zhi, Journal of Biomedical Engineering, Shengwu Yixue Gongchengxue Zazhi
https://www.readbyqxmd.com/read/29761917/cationic-europium-complexes-for-visualizing-fluctuations-in-mitochondrial-atp-levels-in-living-cells
#19
Romain Mailhot, Thomas Traviss-Pollard, Robert Pal, Stephen Jonathan Butler
The ability to study cellular metabolism and enzymatic processes involving adenosine triphosphate (ATP) is impeded by the lack of imaging probes capable of signaling the concentration of intracellular ATP rapidly, with high sensitivity. We report the first example of a luminescent lanthanide complex capable of visualizing changes in the concentration of ATP in the mitochondria of living cells. Four cationic europium(III) complexes [Eu.1-4]+ have been synthesized and their binding affinities towards nucleoside polyphosphate anions examined at physiological pH...
May 15, 2018: Chemistry: a European Journal
https://www.readbyqxmd.com/read/29761528/nonerosive-reflux-disease-clinical-concepts
#20
REVIEW
C Prakash Gyawali, Dan E Azagury, Walter W Chan, Servarayan M Chandramohan, John O Clarke, Nicola de Bortoli, Edgar Figueredo, Mark Fox, Daniela Jodorkovsky, Adriana Lazarescu, Peter Malfertheiner, Jan Martinek, Kenric M Murayama, Roberto Penagini, Edoardo Savarino, Katerina P Shetler, Ellen Stein, Roger P Tatum, Justin Wu
Esophageal symptoms can arise from gastroesophageal reflux disease (GERD) as well as other mucosal and motor processes, structural disease, and functional esophageal syndromes. GERD is the most common esophageal disorder, but diagnosis may not be straightforward when symptoms persist despite empiric acid suppressive therapy and when mucosal erosions are not seen on endoscopy (as for nonerosive reflux disease, NERD). Esophageal physiological tests (ambulatory pH or pH-impedance monitoring and manometry) can be of value in defining abnormal reflux burden and reflux-symptom association...
May 15, 2018: Annals of the New York Academy of Sciences
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