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https://www.readbyqxmd.com/read/28537696/electron-microscopic-changes-of-detrusor-in-benign-enlargement-of-prostate-and-its-clinical-correlation
#1
Sher Singh Yadav, Rohit Bhattar, Lokesh Sharma, Gautam Banga, Trilok Chandra Sadasukhi
AIMS: To study the ultra structural changes in bladder musculature in cases of BPE and their clinical relevance. MATERIAL AND METHODS: In this descriptive longitudinal, controlled, observational study patients were enrolled into three groups, group 1, group 2A and group 2B. Control group (group-1) consisted of age matched normal male patients, who underwent surveillance or diagnostic cystoscopy for microscopic hematuria or irritative symptoms. Case group (group-2) comprised of patients with BPE, undergoing TURP...
May 23, 2017: International Braz J Urol: Official Journal of the Brazilian Society of Urology
https://www.readbyqxmd.com/read/28535452/highly-sensitive-uhplc-ms-ms-method-for-the-simultaneous-estimation-of-propafenone-and-its-metabolites-5-hydroxypropafenone-and-n-depropylpropafenone-on-human-dried-blood-spots-technique-and-application-to-a-pharmacokinetic-study
#2
Adinarayana Andy, Raja Reddy Kallem, Ramesh Mullangi, Divya Andy, J V L N Seshagiri Rao
A highly sensitive, rapid and selective UHPLC-MS/MS method has been developed and validated for quantification of the propafenone (PF), 5-hydroxypropafenone (5-OHPF) and N-depropylpropafenone (N-DPF) on human dried blood spot (DBS). The assay procedure involves a solid-liquid extraction of PF, 5-OHPF and N-DPF and amlodipine (internal standard, I.S.) from dried human DBS cards using water and acetonitrile. The chromatographic resolution was achieved on a BEH C18 column using a gradient mobile phase consisting of 0...
May 11, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28535269/increased-retinal-oxygen-metabolism-precedes-microvascular-alterations-in-type-1-diabetic-mice
#3
Wenzhong Liu, Shoujian Wang, Brian Soetikno, Ji Yi, Kevin Zhang, Siyu Chen, Robert A Linsenmeier, Christine M Sorenson, Nader Sheibani, Hao F Zhang
Purpose: To investigate inner retinal oxygen metabolic rate (IRMRO2) during early stages of type 1 diabetes in a transgenic mouse model. Methods: In current study, we involved seven diabetic mice (Akita/+, TSP1-/-) and seven control mice (TSP1-/-), and applied visible-light optical coherence tomography (vis-OCT) to image functional parameters including retinal blood flow rate, oxygen saturation (sO2) and the IRMRO2 value longitudinally from 5 weeks of age to 13 weeks of age...
February 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/28535094/rheological-and-physical-parameters-correlations-in-formulations-with-pinh%C3%A3-o-derivatives-stability-study-building-up-an-analytical-route
#4
Renata Moschini Daudt, Nilo Sergio Medeiros Cardozo, Ligia Damasceno Ferreira Marczak, Irene Clemes Külkamp Guerreiro
This study focuses on the correlations investigation between rheological and physical parameters and how it can contribute to optimize the topical formulations development. A gel and an emulgel containing pinhão derivatives, and their respective controls, were analyzed along six months of storage. A flowchart of analyses was proposed to use in topical formulation development when a benchmark is the goal or when it is necessary to change some raw material. All formulations were stable over the storage time and the formulations containing pinhão starch and coat extract presented similar properties to those of the control formulations...
May 23, 2017: Pharmaceutical Development and Technology
https://www.readbyqxmd.com/read/28535066/marangoni-flow-induced-evaporation-enhancement-on-binary-sessile-drops
#5
Pin Chen, Souad Harmand, Safouene Ouenzerfi, Jesse Schiffler
The evaporation processes of pure water, pure 1-butanol, and 5% 1-butanol aqueous solution drops on heated hydrophobic substrates are investigated to determine the effect of temperature on the drop evaporation behavior. The evolution of the parameters (contact angle, diameter, and volume) during evaporation measured using a drop shape analyzer and the infrared thermal mapping of the drop surface recorded by an infrared camera were used in investigating the evaporation process. The pure 1-butanol drop does not show any thermal instability at different substrate temperatures, while the convection cells created by the thermal Marangoni effect appear on the surface of the pure water drop from 50°C...
May 23, 2017: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/28534926/asymmetric-bead-aggregation-for-microfluidic-immunodetection
#6
Sunggu Kim, Sanghoon Han, Junghoon Lee
We report an asymmetric immunoaggregation assay for rapid, label-free, and sub-picomolar protein detection. Asymmetric immunoaggregated beads (AIBs) are formed when binding occurs between 1 μm magnetic (MG) and 2.8 μm polystyrene (PS) beads coated with specific antibodies for a target antigen. Detection of such aggregation is achieved by optical monitoring of AIBs in a flow under an external magnetic field. AIBs are attracted to the upper surface of the microchannel by a magnetic field and made to slide along the surface by a flow drag force...
May 23, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28534235/nasal-high-flow-in-management-of-children-with-status-asthmaticus-a-retrospective-observational-study
#7
Florent Baudin, Alexandra Buisson, Blandine Vanel, Bruno Massenavette, Robin Pouyau, Etienne Javouhey
BACKGROUND: Asthma is the most common obstructive airway disease in children and adults. Nasal high flow (NHF) is a recent device that is now used as a primary support for respiratory distress. Several studies have reported use of NHF as a respiratory support in status asthmaticus; however, there are no data to recommend such practice. We therefore conducted this preliminary study to evaluate NHF therapy for children with status asthmaticus admitted to our PICU in order to prepare a multicentre randomized controlled study...
December 2017: Annals of Intensive Care
https://www.readbyqxmd.com/read/28534034/clinical-and-mucosal-immune-correlates-of-hiv-1-semen-levels-in-antiretroviral-naive-men
#8
Brendan J W Osborne, Angie K Marsh, Sanja Huibner, Kamnoosh Shahabi, Cindy Liu, Tania Contente, Nico J D Nagelkerke, Colin Kovacs, Erika Benko, Lance Price, Kelly S MacDonald, Rupert Kaul
BACKGROUND: This study was done to characterize parameters associated with semen human immunodeficiency virus (HIV)-1 ribonucleic acid (RNA) viral load (VL) variability in HIV-infected, therapy-naive men. METHODS: Paired blood and semen samples were collected from 30 HIV-infected, therapy-naive men who have sex with men, and 13 participants were observed longitudinally for up to 1 year. Human immunodeficiency virus RNA, bacterial load by 16S RNA, herpesvirus (Epstein-Barr virus and cytomegalovirus [CMV]) shedding, and semen cytokines/chemokines were quantified, and semen T-cell subsets were assessed by multiparameter flow cytometry...
2017: Open Forum Infectious Diseases
https://www.readbyqxmd.com/read/28532656/the-il-33-st2-axis-is-crucial-in-type-2-airway-responses-induced-by-the-staphylococcus-aureus-protease-spld
#9
Andrea R Teufelberger, Maria Nordengrün, Harald Braun, Tania Maes, Katrien De Grove, Gabriele Holtappels, Clara O'Brien, Sharen Provoost, Hamida Hammad, Amanda Gonçalves, Rudi Beyaert, Wim Declercq, Peter Vandenabeele, Dmitri V Krysko, Barbara M Bröker, Claus Bachert, Olga Krysko
BACKGROUND: Chronic airway inflammatory diseases such as chronic rhinosinusitis with nasal polyps and asthma showed increased nasal Staphylococcus aureus (S. aureus) colonization. Serine protease like protein D (SplD) and other closely related proteases secreted by S. aureus have recently been identified as inducers of allergic asthma in humans and mice but their mechanism of action is largely unknown. OBJECTIVE: We investigated the role of recombinant SplD in driving Th2-biased responses and IgE formation in a murine model of allergic asthma...
May 19, 2017: Journal of Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/28531341/intra-observer-reliability-and-agreement-of-manual-and-digital-orthodontic-model-analysis
#10
Vasiliki Koretsi, Linda Tingelhoff, Peter Proff, Christian Kirschneck
Background and aim: Digital orthodontic model analysis is gaining acceptance in orthodontics, but its reliability is dependent on the digitalisation hardware and software used. We thus investigated intra-observer reliability and agreement / conformity of a particular digital model analysis work-flow in relation to traditional manual plaster model analysis. Materials and methods: Forty-eight plaster casts of the upper/lower dentition were collected. Virtual models were obtained with orthoX®scan (Dentaurum) and analysed with ivoris®analyze3D (Computer konkret)...
May 22, 2017: European Journal of Orthodontics
https://www.readbyqxmd.com/read/28531198/a-real-time-traffic-control-method-for-the-intersection-with-pre-signals-under-the-phase-swap-sorting-strategy
#11
Yiming Bie, Zhiyuan Liu, Yinhai Wang
To deal with the conflicts between left-turn and through traffic streams and increase the discharge capacity, this paper addresses the pre-signal which is implemented at a signalized intersection. Such an intersection with pre-signal is termed as a tandem intersection. For the tandem intersection, phase swap sorting strategy is deemed as the most effective phasing scheme in view of some exclusive merits, such as easier compliance of drivers, and shorter sorting area. However, a major limitation of the phase swap sorting strategy is not considered in previous studies: if one or more vehicle is left at the sorting area after the signal light turns to red, the capacity of the approach would be dramatically dropped...
2017: PloS One
https://www.readbyqxmd.com/read/28530605/screening-of-passiflora-l-mineral-content-using-principal-component-analysis-and-kohonen-self-organizing-maps
#12
Cleber Galvão Novaes, Ivana Lago da Silva Romão, Berlane Gomes Santos, Jéssica Palhares Ribeiro, Marcos Almeida Bezerra, Erik Galvão Paranhos da Silva
A method using digestion with diluted nitric acid and inductively coupled plasma optical emission spectrometry was applied to determine metal ions in the peel, pulp, and seed of Passiflora edulis f. flavicarpa and Passiflora cincinnata cultivated in the Bahia state, Brazil. Plasma parameters (radio frequency power and argon flow rates) were optimized. The quantification limits (µg g(-1)) were: Ca (43.0); Co (1.3); Cu (10.0); Fe (10.0); K (20.0); Mg (26.6); Mn (0.33); Na (56.6) and Zn (10.0). The accuracy of the method was evaluated by the analysis of certified reference materials (NIST 1567a Wheat Flour and NIST 1577b Bovine Liver)...
October 15, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28529646/peptide-vaccination-in-the-presence-of-adjuvants-in-patients-after-hematopoietic-stem-cell-transplantation-with-cd4-t-cell-reconstitution-elicits-consistent-cd8-t-cell-responses
#13
Michael Schmitt, Anita Schmitt, Markus Wiesneth, Angela Hückelhoven, Zeguang Wu, Jürgen Kuball, Lei Wang, Peter Schauwecker, Susanne Hofmann, Marlies Götz, Birgit Michels, Birgit Maccari, Patrick Wuchter, Volker Eckstein, Thomas Mertens, Paul Schnitzler, Hartmut Döhner, Anthony D Ho, Donald W Bunjes, Peter Dreger, Hubert Schrezenmeier, Jochen Greiner
Rationale: Patients receiving an allogeneic stem cell graft from cytomegalovirus (CMV) seronegative donors are particularly prone to CMV reactivation with a high risk of disease and mortality. Therefore we developed and manufactured a novel vaccine and initiated a clinical phase I trial with a CMV phosphoprotein 65 (CMVpp65)-derived peptide. Methods: Ten patients after allogeneic stem cell transplantation received four vaccinations at a biweekly interval. All patients were monitored for CMVpp65 antigenemia...
2017: Theranostics
https://www.readbyqxmd.com/read/28529384/the-pelskin-project-part-v-towards-the-control-of-the-flow-around-aerofoils-at-high-angle-of-attack-using-a-self-activated-deployable-flap
#14
Marco E Rosti, Laura Kamps, Christoph Bruecker, Mohammad Omidyeganeh, Alfredo Pinelli
During the flight of birds, it is often possible to notice that some of the primaries and covert feathers on the upper side of the wing pop-up under critical flight conditions, such as the landing approach or when stalking their prey (see Fig. 1) . It is often conjectured that the feathers pop up plays an aerodynamic role by limiting the spread of flow separation . A combined experimental and numerical study was conducted to shed some light on the physical mechanism determining the feathers self actuation and their effective role in controlling the flow field in nominally stalled conditions...
2017: Meccanica
https://www.readbyqxmd.com/read/28528875/comparison-of-central-and-peripheral-hemodynamics-in-association-with-left-ventricular-diastolic-dysfunction-in-the-community-based-elderly-chinese
#15
Jing Xiong, Xuejing Yu, Shikai Yu, Bin Bai, Henry Xu, Hongwei Ji, Jiadela Teliewubai, Kai Wang, Chen Chi, Yuyan Lu, Yiwu Zhou, Ximin Fan, Jue Li, Yi Zhang, Yawei Xu
The aim of the study was to investigate the association of left ventricular diastolic dysfunction (LVDD) with central and peripheral hemodynamics. A total of 1599 community-based senior residents (aged ≥ 65 years) in northern Shanghai, China, were recruited as of August 2015. Echocardiography was performed for each participant using the MyLab30 Gold CV system. According to the recommendations from the American Society of Echocardiography, the ratio of E (peak early diastolic transmitral flow velocity) to E' (early diastolic lateral mitral annulus velocity) was assessed for the evaluation of LVDD...
April 27, 2017: Journal of the American Society of Hypertension: JASH
https://www.readbyqxmd.com/read/28528845/comparison-of-3-and-7-t-asl-techniques-for-concurrent-functional-perfusion-and-bold-studies
#16
Dimo Ivanov, Anna Gardumi, Roy Am Haast, Josef Pfeuffer, Benedikt A Poser, Kâmil Uludağ
Arterial spin labeling (ASL) is the primary non-invasive MRI approach to measure baseline cerebral blood flow (CBF) in healthy subjects and patients. ASL also allows concurrent functional BOLD signal and CBF measurements, but the latter typically suffers from low contrast-to-noise (CNR) ratio. Ultra-high-field imaging significantly boosts BOLD signal CNR. However, it is contested whether also CBF CNR benefits from increasing magnetic field strength, especially given that technical challenges related to field inhomogeneities and power deposition constraints exist...
May 18, 2017: NeuroImage
https://www.readbyqxmd.com/read/28528294/isoliquiritigenin-reduces-oxidative-damage-and-alleviates-mitochondrial-impairment-by-sirt1-activation-in-experimental-diabetic-neuropathy
#17
Veera Ganesh Yerra, Anil Kumar Kalvala, Ashutosh Kumar
Sirtuin (SIRT1) inactivation underlies the pathogenesis of insulin resistance and hyperglycaemia-associated vascular complications, but its role in diabetic neuropathy (DN) has not been yet explored. We have evaluated hyperglycaemia-induced alteration of SIRT1 signalling and the effect of isoliquiritigenin (ILQ) on SIRT1-directed AMP kinase (AMPK) and peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α) signalling in peripheral nerves of streptozotocin (STZ) (55 mg/kg, ip)-induced diabetic rats and in high glucose (30 mM)-exposed neuro2a (N2A) cells...
May 11, 2017: Journal of Nutritional Biochemistry
https://www.readbyqxmd.com/read/28528156/prognostic-impact-of-low-flow-severe-aortic-stenosis-in-small-body-patients-undergoing-tavr-the-ocean-tavi-registry
#18
Akihisa Kataoka, Yusuke Watanabe, Ken Kozuma, Yugo Nara, Fukuko Nagura, Hideyuki Kawashima, Hirofumi Hioki, Makoto Nakashima, Masanori Yamamoto, Kensuke Takagi, Motoharu Araki, Norio Tada, Shinichi Shirai, Futoshi Yamanaka, Kentaro Hayashida
OBJECTIVES: This study aimed to analyze the prognostic impact of low-flow (LF) severe aortic stenosis in small-body patients undergoing transcatheter aortic valve replacement (TAVR). BACKGROUND: Western literature demonstrates a poor prognosis with paradoxical LF and low-flow low-gradient (LF-LG) severe aortic stenosis (AS), as defined by stroke volume index (SVi) <35 ml/m(2) and mean pressure gradient <40 mm Hg with preserved left ventricular ejection fraction (LVEF)...
May 11, 2017: JACC. Cardiovascular Imaging
https://www.readbyqxmd.com/read/28528092/structural-strength-analysis-of-amorphous-trehalose-maltodextrin-systems
#19
V A Maidannyk, B Nurhadi, Y H Roos
Fundamental knowledge of physical state of materials gives practically important information for food, biological and pharmaceutical industry. Based on Williams-Landel-Ferry (WLF) equation, the strength concept was introduced. This concept provides a simple parameter, S, to express resistance of solids to flow above the glass transition temperature. To develop this approach, miscible trehalose-maltodextrin (0:100; 20:80; 40:60; 60:40; 80:20 and 100:0) systems with different ratios of components were used in the present study...
June 2017: Food Research International
https://www.readbyqxmd.com/read/28527189/patient-specific-3-d-modeling-of-blood-flow-in-a-multi-stenosed-left-coronary-artery
#20
Sarfaraz Kamangar, Irfan Anjum Badruddin, N Ameer Ahamad, Manzoor Elahi M Soudagar, Kalimuthu Govindaraju, N Nik-Ghazali, N J Salman Ahmed, T M Yunus Khan
The current study investigates the effect of multi stenosis on the hemodynamic parameters such as wall pressure, velocity and wall shear stress in the realistic left coronary artery. Patients CT scan image data of normal and diseased left coronary artery was chosen for the reconstruction of 3D coronary artery models. The diseased 3D model of left coronary artery shows a narrowing of more than 70% and 80% of area stenosis (AS) at the left main stem (LMS) and left circumflex (LCX) respectively. The results show that the decrease in pressure was found downstream to the stenosis as compared to the coronary artery without stenosis...
2017: Bio-medical Materials and Engineering
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