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P(v-a)CO2

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https://www.readbyqxmd.com/read/29660859/permafrost-thaw-and-climate-warming-may-decrease-the-co-2-carbon-and-metal-concentration-in-peat-soil-waters-of-the-western-siberia-lowland
#1
T V Raudina, S V Loiko, A Lim, R M Manasypov, L S Shirokova, G I Istigechev, D M Kuzmina, S P Kulizhsky, S N Vorobyev, O S Pokrovsky
Soil pore waters are a vital component of the ecosystem as they are efficient tracers of mineral weathering, plant litter leaching, and nutrient uptake by vegetation. In the permafrost environment, maximal hydraulic connectivity and element transport from soils to rivers and lakes occurs via supra-permafrost flow (i.e. water, gases, suspended matter, and solutes migration over the permafrost table). To assess possible consequences of permafrost thaw and climate warming on carbon and Green House gases (GHG) dynamics we used a "substituting space for time" approach in the largest frozen peatland of the world...
April 11, 2018: Science of the Total Environment
https://www.readbyqxmd.com/read/29659931/shade-compromises-the-photosynthetic-efficiency-of-nadp-me-less-than-pep-ck-and-nad-me-c4-grasses
#2
Balasaheb V Sonawane, Robert E Sharwood, Spencer Whitney, Oula Ghannoum
The high-energy cost and apparently low plasticity of C4 photosynthesis, compared to C3 photosynthesis, may limit the productivity and distribution of C4 plants in low light (LL) environments. C4 photosynthesis evolved numerous times but it remains unclear how different biochemical subtypes perform under LL. We grew eight C4 grasses belonging to three biochemical subtypes (NADP-ME, NAD-ME and PEP-CK) under shade (16% sunlight) or control (full sunlight) conditions and measured their photosynthetic characteristics at both low and high light...
April 5, 2018: Journal of Experimental Botany
https://www.readbyqxmd.com/read/29644221/physiologic-factors-influencing-the-arterial-to-end-tidal-co-2-difference-and-the-alveolar-dead-space-fraction-in-spontaneously-breathing-anesthetised-horses
#3
Martina Mosing, Stephan H Böhm, Anthea Rasis, Giselle Hoosgood, Ulrike Auer, Gerardo Tusman, Regula Bettschart-Wolfensberger, Johannes P Schramel
The arterial to end-tidal CO2 difference (P(a-ET) CO2 ) and alveolar dead space fraction (VDalvfrac  = P(a-ET) CO2 /PaCO2 ), are used to estimate Enghoff's "pulmonary dead space" (V/QEng ), a factor which is also influenced by venous admixture and other pulmonary perfusion abnormalities and thus is not just a measure of dead space as the name suggests. The aim of this experimental study was to evaluate which factors influence these CO2 indices in anesthetized spontaneously breathing horses. Six healthy adult horses were anesthetized in dorsal recumbency breathing spontaneously for 3 h...
2018: Frontiers in Veterinary Science
https://www.readbyqxmd.com/read/29643949/high-secretion-of-interleukin-6-and-increased-mincle-receptor-expression-upon-exposure-to-mycobacterial-cord-factor-analog-trehalose-6-6-dibehenate-tdb-in-patients-with-takayasu-arteritis
#4
Nikhil Gupta, Jayakanthan Kabeerdoss, Hindhumathi Mohan, Ruchika Goel, Debashish Danda
Introduction: Suspicion on the association between Takayasu Arteritis (TA) and Tubcerculosis (TB) has been in vogue for years. Prevalence of TB in TA is reported to be higher. We aimed to study innate immune responses in patients with TA on exposure to Trehalose-6,6-dibehenate (TDB), a synthetic analogue of Trehalose-6,6-Dimycolate (TDM, also known as mycobacterial cord factor) in comparison with healthy controls. Materials and Methods: Patients with type V TA, satisfying 1990 ACR criteria, and age and sex matched healthy controls were recruited...
2018: Open Rheumatology Journal
https://www.readbyqxmd.com/read/29626769/chemical-profiling-of-pm-10-from-urban-road-dust
#5
C A Alves, M Evtyugina, A M P Vicente, E D Vicente, T V Nunes, P M A Silva, M A C Duarte, C A Pio, F Amato, X Querol
Road dust resuspension is one of the main sources of particulate matter with impacts on air quality, health and climate. With the aim of characterising the thoracic fraction, a portable resuspension chamber was used to collect road dust from five main roads in Oporto and an urban tunnel in Braga, north of Portugal. The PM10 samples were analysed for: i) carbonates by acidification and quantification of the evolved CO2 , ii) carbonaceous content (OC and EC) by a thermo-optical technique, iii) elemental composition by ICP-MS and ICP-AES after acid digestion, and iv) organic speciation by GC-MS...
April 4, 2018: Science of the Total Environment
https://www.readbyqxmd.com/read/29625547/endonuclease-activity-of-mutl-protein-of-the-rhodobacter-sphaeroides-mismatch-repair-system
#6
M V Monakhova, A I Penkina, A V Pavlova, A M Lyaschuk, V V Kucherenko, A V Alexeevski, V G Lunin, P Friedhoff, G Klug, T S Oretskaya, E A Kubareva
We have purified the MutL protein from Rhodobacter sphaeroides mismatch repair system (rsMutL) for the first time. rsMutL demonstrated endonuclease activity in vitro, as predicted by bioinformatics analysis. Based on the alignment of 1483 sequences of bacterial MutL homologs with presumed endonuclease activity, conserved functional motifs and amino acid residues in the rsMutL sequence were identified: five motifs comprising the catalytic site responsible for DNA cleavage were found in the C-terminal domain; seven conserved motifs involved in ATP binding and hydrolysis and specific to the GHKL family of ATPases were found in the N-terminal domain...
March 2018: Biochemistry. Biokhimii︠a︡
https://www.readbyqxmd.com/read/29622087/generation-of-venus-fluorochrome-expressing-transgenic-handmade-cloned-buffalo-embryos-using-sleeping-beauty-transposon
#7
Dharmendra Kumar, Papori Sharma, Kennady Vijayalakshmy, Naresh L Selokar, Pradeep Kumar, Rasika Rajendran, P S Yadav
The objective of this study was to optimise the electroporation conditions for efficient integration of Venus construct in buffalo fetal fibroblasts using Sleeping Beauty (SB) based transposition and to produce Venus expressing transgenic cloned embryos through handmade cloning (HMC) approach. Primary culture of buffalo fetal fibroblast cells was established and subsequently cultured cells were co-transfected with Venus and helper plasmid at different combinations of electroporation condition. In different combinations of voltage, time and plasmid dose, we observed that 300 V, single pulse for 10 ms in 2 mm cuvette and 1...
April 2018: Tissue & Cell
https://www.readbyqxmd.com/read/29600896/wavelet-decomposition-analysis-is-a-clinically-relevant-strategy-to-evaluate-cerebrovascular-buffering-of-blood-pressure-after-spinal-cord-injury
#8
Saqib Saleem, Diana Vucina, Zoe Sarafis, Amanda H X Lee, Jordan W Squair, Otto F Barak, Geoff B Coombs, Tanja Mijacika, Andrei V Krassioukov, Philip N Ainslie, Zeljko Dujic, Yu-Chieh Tzeng, Aaron A Phillips
The capacity of cerebrovasculature to buffer changes in blood pressure (BP) is crucial to prevent stroke, the incidence of which is 3-4 fold elevated after spinal cord injury (SCI). Disruption of descending sympathetic pathways within the spinal cord due to cervical SCI may result in impaired cerebrovascular buffering. Only linear analyses of cerebrovascular buffering of BP, such as transfer function, have been utilized in SCI research. This approach does not account for inherent non-linearity and non-stationarity components of cerebrovascular regulation, often depends on perturbations of BP to increase the statistical power, and does not account for influence of arterial CO2 tension...
March 30, 2018: American Journal of Physiology. Heart and Circulatory Physiology
https://www.readbyqxmd.com/read/29591780/a-comparison-of-the-effects-of-carbon-dioxide-and-medical-air-for-abdominal-insufflation-on-respiratory-parameters-in-xylazine-sedated-sheep-undergoing-laparoscopic-artificial-insemination
#9
J D Haan, B L Hay Kraus, S R Sathe
AIMS: To determine if abdominal insufflation with medical air will improve oxygenation and ventilation parameters when compared to insufflation with CO2 in xylazine-sedated sheep undergoing laparoscopic artificial insemination (AI). METHODS: Forty-seven sheep underwent oestrus synchronisation and were fasted for 24 hours prior to laparoscopic AI. Each animal was randomised to receive either CO2 or medical air for abdominal insufflation. An auricular arterial catheter was placed and utilised for serial blood sampling...
March 28, 2018: New Zealand Veterinary Journal
https://www.readbyqxmd.com/read/29559349/molecular-characterization-light-dependent-expression-and-cellular-localization-of-a-host-vacuolar-type-h-atpase-vha-subunit-a-in-the-giant-clam-tridacna-squamosa-indicate-the-involvement-of-the-host-vha-in-the-uptake-of-inorganic-carbon-and-its-supply-to-the
#10
Yuen K Ip, Kum C Hiong, Leon J Y Lim, Celine Y L Choo, Mel V Boo, Wai P Wong, Mei L Neo, Shit F Chew
The giant clam, Tridacna squamosa, represents a clam-zooxanthellae association. In light, the host clam and the symbiotic zooxanthellae conduct light-enhanced calcification and photosynthesis, respectively. We had cloned the cDNA coding sequence of a Vacuolar-type Proton ATPase (VHA) subunit A, ATP6V1A, from T. squamosa, whereby the VHA is an electrogenic transporter that actively 'pumps' H+ out of the cell. The ATP6V1A of T. squamosa comprised 1866 bp, encoding a protein of 622 amino acids and 69.9 kDa, and has a host-origin...
March 17, 2018: Gene
https://www.readbyqxmd.com/read/29557514/outcomes-of-co-2-laser-assisted-posterior-cordectomy-in-bilateral-vocal-cord-paralysis-in-132-cases
#11
Joanna Jackowska, Elisabeth V Sjogren, Anna Bartochowska, Hanna Czerniejewska-Wolska, Krzysztof Piersiala, Malgorzata Wierzbicka
The purpose of the study was to assess the role of laser-assisted posterior cordectomy in the management of patients with bilateral vocal cord paralysis. We aimed an analysis of 132 consecutive patients treated by CO2 laser posterior cordectomy, aged 38-91, 31% tracheotomized on admission. Cordectomy was performed under microlaryngoscopy using CO2 laser (Lumenis AcuPulse 40 CO2 laser, wavelength 10.6 μm, Lumenis Ltd., Yokneam, Israel). We looked at the number of laser glottic procedures necessary to achieve decannulation in tracheotomized patients and to achieve respiratory comfort in non-tracheotomized subjects and we evaluated the two groups for differences in patient characteristics...
March 20, 2018: Lasers in Medical Science
https://www.readbyqxmd.com/read/29554779/carbon-burial-and-storage-in-tropical-salt-marshes-under-the-influence-of-sea-level-rise
#12
A C Ruiz-Fernández, V Carnero-Bravo, J A Sanchez-Cabeza, L H Pérez-Bernal, O A Amaya-Monterrosa, S Bojórquez-Sánchez, P G López-Mendoza, J G Cardoso-Mohedano, R B Dunbar, D A Mucciarone, A J Marmolejo-Rodríguez
Coastal vegetated habitats can be important sinks of organic carbon (Corg ) and mitigate global warming by sequestering significant quantities of atmospheric CO2 and storing sedimentary Corg for long periods, although their Corg burial and storage capacity may be affected by on-going sea level rise and human intervention. Geochemical data from published210 Pb-dated sediment cores, collected from low-energy microtidal coastal wetlands in El Salvador (Jiquilisco Bay) and in Mexico (Salada Lagoon; Estero de Urias Lagoon; Sian Ka'an Biosphere Reserve) were revisited to assess temporal changes (within the last 100years) of Corg concentrations, storage and burial rates in tropical salt marshes under the influence of sea level rise and contrasting anthropization degree...
March 13, 2018: Science of the Total Environment
https://www.readbyqxmd.com/read/29540461/light-exposure-enhances-urea-absorption-in-the-fluted-giant-clam-tridacna-squamosa-and-up-regulates-the-protein-abundance-of-a-light-dependent-urea-active-transporter-dur3-like-in-its-ctenidium
#13
Christabel Y L Chan, Kum C Hiong, Mel V Boo, Celine Y L Choo, Wai P Wong, Shit F Chew, Yuen K Ip
Giant clams live in nutrient-poor reef waters of the Indo-Pacific and rely on symbiotic dinoflagellates ( Symbiodinium spp., also known as zooxanthellae) for nutrients. As the symbionts are nitrogen deficient, the host clam has to absorb exogenous nitrogen and supply it to them. This study aimed to demonstrate light-enhanced urea absorption in the fluted giant clam, Tridacna squamosa , and to clone and characterize the urea active transporter, DUR3-like, from its ctenidium (gill). Results indicate that T. squamosa could absorb exogenous urea, and the rate of urea uptake in light was significantly higher than that in darkness...
March 14, 2018: Journal of Experimental Biology
https://www.readbyqxmd.com/read/29532820/multi-channel-dynamics-in-high-harmonic-generation-of-aligned-co-2-ab-initio-analysis-with-time-dependent-b-spline-algebraic-diagrammatic-construction
#14
M Ruberti, P Decleva, V Averbukh
Here we present a fully ab initio study of the high-order harmonic generation (HHG) spectrum of aligned CO2 molecules. The calculations have been performed by using the molecular time-dependent (TD) B-spline algebraic diagrammatic construction (ADC) method. We quantitatively study how the sub-cycle laser-driven multi-channel dynamics, as reflected in the position of the dynamical minimum in the HHG spectrum, is affected by the full inclusion of both correlation-driven and laser-driven dipole interchannel couplings...
March 13, 2018: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/29531221/evidence-for-ephemeral-middle-eocene-to-early-oligocene-greenland-glacial-ice-and-pan-arctic-sea-ice
#15
Aradhna Tripati, Dennis Darby
Earth's modern climate is defined by the presence of ice at both poles, but that ice is now disappearing. Therefore understanding the origin and causes of polar ice stability is more critical than ever. Here we provide novel geochemical data that constrain past dynamics of glacial ice on Greenland and Arctic sea ice. Based on accurate source determinations of individual ice-rafted Fe-oxide grains, we find evidence for episodic glaciation of distinct source regions on Greenland as far-ranging as ~68°N and ~80°N synchronous with ice-rafting from circum-Arctic sources, beginning in the middle Eocene...
March 12, 2018: Nature Communications
https://www.readbyqxmd.com/read/29504251/identification-and-characterization-of-a-novel-alkalistable-and-salt-tolerant-esterase-from-the-deep-sea-hydrothermal-vent-of-the-east-pacific-rise
#16
Xinwei Yang, Lianzuan Wu, Ying Xu, Chongrong Ke, Fangfang Hu, Xiang Xiao, Jianzhong Huang
A novel esterase gene selected from metagenomic sequences of deep-sea hydrothermal vents was successfully expressed in Escherichia coli. The recombinant protein (est-OKK), which belongs to the lipolytic enzyme family V, exhibited high activity toward pNP-esters with short acyl chains and especially p-nitrophenyl butyrate. Site-mutagenesis results confirmed that est-OKK contains the nonclassical catalytic tetrad predicted by alignment and computational modeling. The est-OKK protein is a moderately thermophilic enzyme that is relatively thermostable, and highly salt-tolerant, which remained stable in 3 mol/L NaCl for 6 hr...
March 5, 2018: MicrobiologyOpen
https://www.readbyqxmd.com/read/29492549/effect-of-mild-hypocapnia-on-critical-closing-pressure-and-other-mechanoelastic-parameters-of-the-cerebrospinal-system
#17
Peter Smielewski, Luzius Steiner, Corina Puppo, Karol Budohoski, Georgios V Varsos, Marek Czosnyka
OBJECTIVE: Brain arterial critical closing pressure (CrCP) has been studied in several diseases such as traumatic brain injury (TBI), subarachnoid haemorrhage, hydrocephalus, and in various physiological scenarios: intracranial hypertension, decreased cerebral perfusion pressure, hypercapnia, etc. Little or nothing so far has been demonstrated to characterise change in CrCP during mild hypocapnia. METHOD: We retrospectively analysed recordings of intracranial pressure (ICP), arterial blood pressure (ABP) and blood flow velocity from 27 severe TBI patients (mean 39...
2018: Acta Neurochirurgica. Supplement
https://www.readbyqxmd.com/read/29477729/slow-v%C3%AC-o-2-kinetics-in-acute-hypoxia-are-not-related-to-a-hyperventilation-induced-hypocapnia
#18
Daniel A Keir, Michael Pollock, Piramilan Thuraisingam, Donald H Paterson, George J F Heigenhauser, Harry B Rossiter, John M Kowalchuk
We examined whether slower pulmonary O2 uptake (V̇O2p ) kinetics in hypoxia is a consequence of: a) hypoxia alone (lowered arterial O2 pressure), b) hyperventilation-induced hypocapnia (lowered arterial CO2 pressure), or c) a combination of both. Eleven participants performed 3-5 repetitions of step-changes in cycle ergometer power output from 20W to 80% lactate threshold in the following conditions: i) normoxia (CON; room air); ii) hypoxia (HX, inspired O2  = 12%; lowered end-tidal O2 pressure [PET O2 ] and end-tidal CO2 pressure [PET CO2 ]); iii) hyperventilation (HV; increased PET O2 and lowered PET CO2 ); and iv) normocapnic hypoxia (NC-HX; lowered PET O2 and PET CO2 matched to CON)...
February 22, 2018: Respiratory Physiology & Neurobiology
https://www.readbyqxmd.com/read/29411877/contrasting-acclimation-abilities-of-two-dominant-boreal-conifers-to-elevated-co2-and-temperature
#19
Leonid V Kurepin, Zsofia R Stangl, Alexander G Ivanov, Vi Bui, Marin Mema, Norman P A Hüner, Gunnar Öquist, Danielle Way, Vaughan Hurry
High latitude forests will experience large changes in temperature and CO2 concentrations this century. We evaluated the effects of future climate conditions on two dominant boreal tree species, Pinus sylvestris L. and Picea abies (L.) H. Karst, exposing seedlings to three seasons of ambient (430 ppm) or elevated CO2 (750 ppm) and ambient temperatures, a +4 °C warming, or a +8 °C warming. Pinus sylvestris responded positively to warming: seedlings developed a larger canopy, maintained high net CO2 assimilation rates (Anet ) and acclimated dark respiration (Rdark )...
February 7, 2018: Plant, Cell & Environment
https://www.readbyqxmd.com/read/29400450/multifunctional-metal-organic-frameworks-based-on-redox-active-rhenium-octahedral-clusters
#20
Yulia M Litvinova, Yakov M Gayfulin, Konstantin A Kovalenko, Denis G Samsonenko, Jan van Leusen, Ilya V Korolkov, Vladimir P Fedin, Yuri V Mironov
The redox-active rhenium octahedral cluster unit [Re6Se8(CN)6]4- was combined with Gd3+ ions and dicarboxylate linkers in novel types of metal-organic frameworks (MOFs) that display a set of functional properties. The hydrolytically stable complexes [{Gd(H2O)3}2(L)Re6Se8(CN)6]·nH2O (1, L = furan-2,5-dicarboxylate, fdc; 2, L = thiophene-2,5-dicarboxylate, tdc) exhibit a 3D framework of trigonal symmetry where 1D chains of [{Gd(H2O)3}2(L)]4+ are connected by [Re6Se8(CN)6]4- clusters. Frameworks contain spacious channels filled with H2O...
February 5, 2018: Inorganic Chemistry
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