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https://www.readbyqxmd.com/read/28075062/specific-arabidopsis-thaliana-malic-enzyme-isoforms-can-provide-anaplerotic-pyruvate-carboxylation-function-in-saccharomyces-cerevisiae
#1
Mariana Beatriz Badia, Robert Mans, Alicia V Lis, Marcos Ariel Tronconi, Cintia Lucía Arias, Verónica Graciela Maurino, Carlos Santiago Andreo, María Fabiana Drincovich, Antonius J A van Maris, Mariel Claudia Gerrard Wheeler
NAD(P)-malic enzyme (NAD(P)-ME) catalyzes the reversible oxidative decarboxylation of malate to pyruvate, CO2 and NAD(P)H and is present as a multigene family in Arabidopsis. The carboxylation reaction catalyzed by purified recombinant A. thaliana NADP-ME proteins is faster than those reported for other animal or plant isoforms. In contrast, no carboxylation activity could be detected in vitro for the NAD-dependent counterparts. In order to further investigate their putative carboxylating role in vivo, Arabidopsis NAD(P)-ME isoforms, as well as the NADP-ME2del2 (with a decreased ability to carboxylate pyruvate) and NADP-ME2R115A (lacking fumarate activation) versions, were functionally expressed in the cytosol of pyruvate carboxylase negative (Pyc(-) ) Saccharomyces cerevisiae strains...
January 11, 2017: FEBS Journal
https://www.readbyqxmd.com/read/28058442/%C3%AF-phosphorene-a-new-allotrope-of-phosphorene
#2
Haidi Wang, Xingxing Li, Zhao Liu, Jinlong Yang
Based on the crystal structure prediction, we propose a new allotrope of phosphorene, ψ-phosphorene (ψ-P), with a porous structure, which is both thermally and dynamically stable in comparison with the previously reported allotropes. Due to its unique atom configuration, ψ-P has highly orientation-dependent mechanical properties and excellent flexibility. Calculations using the HSE functional predict that ψ-P is semiconducting with an indirect band gap of 1.57 eV and possesses anisotropic transport properties...
January 6, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/28049739/outside-xylem-vulnerability-not-xylem-embolism-controls-leaf-hydraulic-decline-during-dehydration
#3
Christine Scoffoni, Caetano Albuquerque, Craig Brodersen, Shatara V Townes, Grace P John, Megan K Bartlett, Thomas N Buckley, Andrew J McElrone, Lawren Sack
Leaf hydraulic supply is crucial to maintaining open stomata for CO2 capture and plant growth. During drought-induced dehydration, the leaf hydraulic conductance (Kleaf) declines, which contributes to stomatal closure and eventually to leaf death. Previous studies have tended to attribute the decline of Kleaf to embolism in the leaf vein xylem. We visualized at high resolution and quantified experimentally the hydraulic vulnerability of xylem and outside-xylem pathways and modelled their respective influences on plant water transport...
January 3, 2017: Plant Physiology
https://www.readbyqxmd.com/read/28043691/the-joule-thomson-coefficient-as-a-criterion-for-efficient-operating-conditions-in-supercritical-fluid-chromatography
#4
Donald P Poe, Shawn Helmueller, Stephanie Kobany, Hannah Feldhacker, Krzysztof Kaczmarski
When an SFC column is operated in a traditional oven with forced air at low pressures near the critical temperature, severe efficiency losses can occur. The mobile phase cools as it expands along the column, forming axial and radial temperature gradients. In this study we present a simple model based on a virtual fluid to predict the conditions which lead to the onset of efficiency loss. The model shows that the Joule-Thomson coefficient is an important factor leading to efficiency loss in packed columns under forced air conditions...
December 23, 2016: Journal of Chromatography. A
https://www.readbyqxmd.com/read/28013141/closed-nutrient-recycling-via-microbial-catabolism-in-an-eco-engineered-self-regenerating-mixed-anaerobic-microbiome-for-hydrogenotrophic-methanogenesis
#5
Savvas Savvas, Joanne Donnelly, Tim P Patterson, Richard Dinsdale, Sandra R Esteves
A novel eco-engineered mixed anaerobic culture was successfully demonstrated for the first time to be capable of continuous regeneration in nutrient limiting conditions. Microbial catabolism has been found to support a closed system of nutrients able to enrich a culture of lithotrophic methanogens and provide microbial cell recycling. After enrichment, the hydrogenotrophic species was the dominating methanogens while a bacterial substratum was responsible for the redistribution of nutrients. q-PCR results indicated that 7% of the total population was responsible for the direct conversion of the gases...
March 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28010843/an-in-source-multiple-collision-neutral-loss-filtering-based-nontargeted-metabolomics-approach-for-the-comprehensive-analysis-of-malonyl-ginsenosides-from-panax-ginseng-p-%C3%A2-quinquefolius-and-p-%C3%A2-notoginseng
#6
Xiao-Jian Shi, Wen-Zhi Yang, Shi Qiu, Chang-Liang Yao, Yao Shen, Hui-Qin Pan, Qi-Rui Bi, Min Yang, Wan-Ying Wu, De-An Guo
The simultaneous identification and quantification of target metabolites from herbal medicines are difficult to implement by the full-scan MS based nontargeted metabolomics approaches. Here an in-source multiple collision-neutral loss filtering (IMC-NLF) based nontargeted metabolomics approach is developed and applied to identify and quantify the variations of malonyl-ginsenosides, a common group of acyl saponins with potential anti-diabetic activity, among Panax ginseng, P. quinquefolius, and P. notoginseng...
February 1, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28010808/acute-effects-of-temperature-and-hypercarbia-on-cutaneous-and-branchial-gas-exchange-in-the-south-american-lungfish-lepidosiren-paradoxa
#7
Lucas A Zena, Kênia C Bícego, Glauber S F da Silva, Humberto Giusti, Mogens L Glass, Adriana P Sanchez
The South American lungfish, Lepidosiren paradoxa inhabits seasonal environments in the Central Amazon and Paraná-Paraguay basins that undergo significant oscillations in temperature throughout the year. They rely on different gas exchange organs, such as gills and skin for aquatic gas exchange while their truly bilateral lungs are responsible for aerial gas exchange; however, there are no data available on the individual contributions of the skin and the gills to total aquatic gas exchange in L. paradoxa...
January 2017: Journal of Thermal Biology
https://www.readbyqxmd.com/read/28003557/comparing-the-effects-of-two-different-levels-of-hyperoxygenation-on-gas-exchange-during-open-endotracheal-suctioning-a-randomized-crossover-study
#8
Jacqueline Rodrigues de Freitas Vianna, Valéria Amorim Pires Di Lorenzo, Miléa Mara Lourenço da Silva Simões, Maurício Jamami
BACKGROUND: Endotracheal suctioning is required for mechanically ventilated patients to maintain a patent airway. Studies show that open endotracheal suctioning affects respiratory mechanics and gas exchange. The aim of this study was to compare the effectiveness of hyperoxygenation with FIO2 + 0.20 above baseline and hyperoxygenation with FIO2 1.0 in preventing hypoxemia, and to determine the impact of open endotracheal suctioning on the pulmonary ventilation of critical subjects receiving mechanical ventilatory support...
January 2017: Respiratory Care
https://www.readbyqxmd.com/read/27997255/emphysema-on-thoracic-ct-and-exercise-ventilatory-inefficiency-in-mild-to-moderate-copd
#9
Joshua H Jones, Joel T Zelt, Daniel M Hirai, Camilla V Diniz, Aida Zaza, Denis E O'Donnell, J Alberto Neder
There is growing evidence that emphysema on thoracic computed tomography (CT) is associated with poor exercise tolerance in COPD patients with only mild-to-moderate airflow obstruction. We hypothesized that an excessive ventilatory response to exercise (ventilatory inefficiency) would underlie these abnormalities. In a prospective study, 19 patients (FEV1 = 82 ± 13%, 12 Global Initiative for Chronic Obstructive Lung Disease (GOLD) stage 1) and 26 controls underwent an incremental exercise test. Ventilatory inefficiency was assessed by the ventilation ([Formula: see text]E)/CO2 output ([Formula: see text]CO2) nadir...
December 20, 2016: COPD
https://www.readbyqxmd.com/read/27995188/does-carbon-dioxide-insufflation-impact-adenoma-detection-rate-a-single-center-retrospective-analysis
#10
Yaseen B Perbtani, Michael Riverso, Jonathan J Shuster, Joydeep Chakraborty, Tony S Brar, Mitali Agarwal, Han Zhang, Anand Gupte, Shailendra S Chauhan, Christopher E Forsmark, Peter V Draganov, Dennis Yang
Background and study aims: Carbon dioxide (CO2) has been associated with reduced post-procedural pain and improved patient satisfaction when compared to air insufflation (AI). The effect of CO2 insufflation (CO2I) on the adenoma detection rate (ADR) remains unclear. The aims of this study are to compare ADR in patients undergoing screening colonoscopy with AI vs. CO2I and identify predictors of ADR. Patients and methods: Single-center retrospective cohort study of 2,107 patients undergoing screening colonoscopy at the University of Florida Hospital between November 2011 and June 2015...
December 2016: Endoscopy International Open
https://www.readbyqxmd.com/read/27989199/manganese-tricarbonyl-complexes-with-asymmetric-2-iminopyridine-ligands-toward-decoupling-steric-and-electronic-factors-in-electrocatalytic-co2-reduction
#11
Steven J P Spall, Theo Keane, Joanne Tory, Dean C Cocker, Harry Adams, Hannah Fowler, Anthony J H M Meijer, František Hartl, Julia A Weinstein
Manganese tricarbonyl bromide complexes incorporating IP (2-(phenylimino)pyridine) derivatives, [MnBr(CO)3(IP)], are demonstrated as a new group of catalysts for CO2 reduction, which represent the first example of utilization of (phenylimino)pyridine ligands on manganese centers for this purpose. The key feature is the asymmetric structure of the redox-noninnocent ligand that permits independent tuning of its steric and electronic properties. The α-diimine ligands and five new Mn(I) compounds have been synthesized, isolated in high yields, and fully characterized, including X-ray crystallography...
December 19, 2016: Inorganic Chemistry
https://www.readbyqxmd.com/read/27986845/unique-photosynthetic-phenotypes-in-portulaca-portulacaceae-c3-c4-intermediates-and-nad-me-c4-species-with-pilosoid-type-kranz-anatomy
#12
Elena V Voznesenskaya, Nuria K Koteyeva, Gerald E Edwards, Gilberto Ocampo
Portulacaceae is a family that has considerable diversity in photosynthetic phenotypes. It is one of 19 families of terrestrial plants where species having C4 photosynthesis have been found. Most species in Portulaca are in the alternate-leaved (AL) lineage, which includes one clade (Cryptopetala) with taxa lacking C4 photosynthesis and three clades having C4 species (Oleracea, Umbraticola and Pilosa). All three species in the Cryptopetala clade lack Kranz anatomy, the leaves have C3-like carbon isotope composition and they have low levels of C4 cycle enzymes...
December 16, 2016: Journal of Experimental Botany
https://www.readbyqxmd.com/read/27965366/expression-of-cyanobacterial-fbp-sbpase-in-soybean-prevents-yield-depression-under-future-climate-conditions
#13
Iris H Köhler, Ursula M Ruiz-Vera, Andy VanLoocke, Michell L Thomey, Tom Clemente, Stephen P Long, Donald R Ort, Carl J Bernacchi
Predictions suggest that current crop production needs to double by 2050 to meet global food and energy demands. Based on theory and experimental studies, overexpression of the photosynthetic enzyme sedoheptulose-1,7-bisphosphatase (SBPase) is expected to enhance C3 crop photosynthesis and yields. Here we test how expression of the cyanobacterial, bifunctional fructose-1,6/sedoheptulose-1,7-bisphosphatase (FBP/SBPase) affects carbon assimilation and seed yield (SY) in a major crop (soybean, Glycine max). For three growing seasons, wild-type (WT) and FBP/SBPase-expressing (FS) plants were grown in the field under ambient (400 μmol mol(-1)) and elevated (600 μmol mol(-1)) CO2 concentrations [CO2] and under ambient and elevated temperatures (+2...
December 12, 2016: Journal of Experimental Botany
https://www.readbyqxmd.com/read/27957304/life-expectancy-and-its-socioeconomic-determinants-in-iran
#14
Somayeh Delavari, Hamed Zandian, Satar Rezaei, Mehdi Moradinazar, Sajad Delavari, Ali Saber, Razieh Fallah
INTRODUCTION: Life expectancy at birth (LEB) is closely associated with the degree of economic and social development in developed and developing countries. This study aimed to examine the socioeconomic factors affecting LEB in Iran from 1985 to 2013. METHODS: Time series analysis was used to examine the effects of key explanatory factors (GDP per capita, number of doctors per 10,000 population, degree of urbanization, food availability, CO2 emission, total fertility rate, inflation rate, and literacy rate) on LEB in Iran from 1985 to 2013...
October 2016: Electronic Physician
https://www.readbyqxmd.com/read/27936430/collagen-tissue-treated-with-chitosan-solution-in-h2o-co2-mixtures-influence-of-clathrates-hydrates-on-the-structure-and-mechanical-properties
#15
Ivan S Chaschin, Natalia P Bakuleva, Timofei E Grigoriev, Sergey V Krasheninnikov, Lev N Nikitin
A mixture of water/carbon dioxide is a "green" perspective solvent from the viewpoint of biomedical applications. Clathrate hydrates are formed this solvent under certain conditions and a very interesting question is the impact of clathrates hydrates on the structure and properties of bovine pericardium, which is used in biomedicine, in particular as a main part of biological heart valve prostheses. The aim of the present work is to investigate the influence of clathrates on the structure and mechanical properties of the collagen tissue treated with chitosan in H2O/CO2 mixtures under pressure 3...
November 30, 2016: Journal of the Mechanical Behavior of Biomedical Materials
https://www.readbyqxmd.com/read/27933832/annealing-based-electrical-tuning-of-cobalt-carbon-deposits-grown-by-focused-electron-beam-induced-deposition
#16
Marcos V Puydinger Dos Santos, Murilo F Velo, Renan D Domingos, Yucheng Zhang, Xavier Maeder, Carlos Guerra-Nuñez, James P Best, Fanny Béron, Kleber R Pirota, Stanislav Moshkalev, José A Diniz, Ivo Utke
An effective postgrowth electrical tuning, via an oxygen releasing method, to enhance the content of non-noble metals in deposits directly written with gas-assisted focused-electron-beam-induced deposition (FEBID) is presented. It represents a novel and reproducible method for improving the electrical transport properties of Co-C deposits. The metal content and electrical properties of Co-C-O nanodeposits obtained by electron-induced dissociation of volatile Co2(CO)8 precursor adsorbate molecules were reproducibly tuned by applying postgrowth annealing processes at 100 °C, 200 °C, and 300 °C under high-vacuum for 10 min...
November 30, 2016: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/27927624/cerebral-blood-flow-autoregulation-in-ischemic-heart-failure
#17
Juliana R Caldas, Ronney B Panerai, Victoria Jane Haunton, Juliano P Almeida, Graziela S R Ferreira, Ligia Camara, Ricardo de Carvalho Nogueira, Edson Bor-Seng-Shu, Marcelo L Oliveira, Raphaela R V Groehs, Larissa Ferreira-Santos, Manoel Jacobsen Teixeira, Filomena R B G Galas, Thompson G Robinson, Fabio B Jatene, Ludhmila A Hajjar
Patients with ischemic heart failure (iHF) have a high risk of neurological complications such as cognitive impairment and stroke. We hypothesized that iHF patients have a higher incidence of impaired dynamic cerebral autoregulation (dCA). Adult patients with iHF and healthy volunteers were included. Cerebral blood flow velocity (CBFV, transcranial Doppler, middle cerebral artery), end-tidal CO2 (capnography), and arterial blood pressure (Finometer) were continuously recorded supine for five minutes at rest...
December 7, 2016: American Journal of Physiology. Regulatory, Integrative and Comparative Physiology
https://www.readbyqxmd.com/read/27925360/hierarchical-mesoporous-sno2-nanosheets-on-carbon-cloth-a-robust-and-flexible-electrocatalyst-for-co2-reduction-with-high-efficiency-and-selectivity
#18
Fengwang Li, Lu Chen, Gregory P Knowles, Douglas R MacFarlane, Jie Zhang
Electrochemical reduction of CO2 into liquid fuels is a promising approach to achieve a carbon-neutral energy cycle. However, conventional electrocatalysts usually suffer from low energy efficiency and poor selectivity and stability. A 3D hierarchical structure composed of mesoporous SnO2 nanosheets on carbon cloth is proposed to efficiently and selectively electroreduce CO2 to formate in aqueous media. The electrode is fabricated by a facile combination of hydrothermal reaction and calcination. It exhibits an unprecedented partial current density of about 45 mA cm(-2) at a moderate overpotential (0...
January 9, 2017: Angewandte Chemie
https://www.readbyqxmd.com/read/27911498/solvothermally-prepared-cu2o-electrocatalysts-for-co2-reduction-with-tunable-selectivity-by-the-introduction-of-p-block-elements
#19
Gastón O Larrazábal, Antonio J Martín, Frank Krumeich, Roland Hauert, Javier Pérez-Ramírez
The electroreduction of CO2 to fuels and chemicals is an attractive strategy for valorizing CO2 emissions. In this study, we show that a Cu2O electrocatalyst prepared by a simple and potentially scalable solvothermal route effectively targeted CO evolution at low to moderate overpotentials (CECO ca. 60% after 12 h at -0.6 V vs. RHE), and that the introduction of p-block elements (In, Sn, Ga, Al) into the catalyst can be employed to tune its selectivity. SEM, HRTEM, and voltammetric analyses revealed that the Cu2O catalyst undergoes extensive surface restructuring (favorable for CO evolution) under reaction conditions...
December 2, 2016: ChemSusChem
https://www.readbyqxmd.com/read/27898193/modeling-long-term-changes-in-tundra-carbon-balance-following-wildfire-climate-change-and-potential-nutrient-addition
#20
Yueyang Jiang, Edward B Rastetter, Gaius R Shaver, Adrian V Rocha, Qianlai Zhuang, Bonnie L Kwiatkowski
To investigate the underlying mechanisms that control long-term recovery of tundra carbon (C) and nutrients after fire, we employed the Multiple Element Limitation (MEL) model to simulate 200-yr post-fire changes in the biogeochemistry of three sites along a burn severity gradient in response to increases in air temperature, CO2 concentration, nitrogen (N) deposition, and phosphorus (P) weathering rates. The simulations were conducted for severely burned, moderately burned, and unburned arctic tundra. Our simulations indicated that recovery of C balance after fire was mainly determined by the internal redistribution of nutrients among ecosystem components (controlled by air temperature), rather than the supply of nutrients from external sources (e...
August 4, 2016: Ecological Applications: a Publication of the Ecological Society of America
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