Daniel J Tyrrell, Judy Chen, Benjamin Y Li, Sherri C Wood, Wendy Rosebury-Smith, Henriette A Remmer, Longtan Jiang, Min Zhang, Morgan Salmon, Gorav Ailawadi, Bo Yang, Daniel R Goldstein
BACKGROUND: Aging enhances most chronic diseases but its impact on human aortic tissue in health and in thoracic aortic aneurysms (TAA) remains unclear. METHODS: We employed a human aortic biorepository of healthy specimens (n=17) and those that underwent surgical repair for TAA (n=20). First, we performed proteomics comparing aortas of healthy donors to aneurysmal specimens, in young (ie, <60 years of age) and old (ie, ≥60 years of age) subjects. Second, we measured proteins, via immunoblotting, involved in mitophagy (ie, Parkin) and also mitochondrial-induced inflammatory pathways, specifically TLR (toll-like receptor) 9, STING (stimulator of interferon genes), and IFN (interferon)-β...
May 5, 2022: Arteriosclerosis, Thrombosis, and Vascular Biology