Karen J Thompson, Sonia Watson, Chiara Zanato, Sergio Dall'Angelo, Joriene C De Nooij, Bethany Pace-Bonello, Fiona C Shenton, Helen E Sanger, Beverly A Heinz, Lisa M Broad, Noelle Grosjean, Jessica R McQuillian, Marina Dubini, Susan Pyner, Iain Greig, Matteo Zanda, David Bleakman, Robert W Banks, Guy S Bewick
A metabotropic glutamate receptor coupled to phospholipase D (PLD-mGluR) was discovered in the hippocampus over three decades ago. Its pharmacology and direct linkage to PLD activation are well established and indicate it is a highly atypical glutamate receptor. A receptor with the same pharmacology is present in spindle primary sensory terminals where its blockade can totally abolish, and its activation can double, the normal stretch-evoked firing. We report here the first identification of this PLD-mGluR protein, by capitalizing on its expression in primary mechanosensory terminals, developing an enriched source, pharmacological profiling to identify an optimal ligand, and then functionalizing it as a molecular tool...
September 1, 2023: Experimental Physiology