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https://www.readbyqxmd.com/read/28636231/orchestration-at-the-beginning-mitosis-in-sea-urchin-embryo
#1
Giselle A Abruzzese, Nae Tanpradit, Renata S Tavares
Development of multicellular organisms is driven by a series of mitotic divisions after fertilization. Total cytoplasmic volume does not increase during this process, resulting in an increasing number of smaller daughter cells. Cell divisions are initially synchronous in an early embryo, but this ends at the 16-cell stage in the sea urchin. Orchesterated mitosis does, however, continue in subpopulations via the coordination of two different processes: karyokinesis and cytokinesis. Part of this asynchrony is due to the differentiation of lineages...
June 21, 2017: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/28584846/rare-esophageal-migration-of-axios-stent-used-for-walled-off-pancreatic-necrosis-drainage
#2
Yousef Abdel-Aziz, Anas Renno, Tariq Hammad, Ali Nawras
The AXIOS stent (Boston Scientific, Marlborough, MA) is a novel lumen-apposing self-expandable metallic stent designed for enteric drainage of nonadherent lumens. Efficacy and safety of using the AXIOS stent for pancreatic fluid drainage have been consistently shown in several studies. Although it is less common with this novel stent, stent migration still may happen. We present a case of AXIOS stent migration into the esophagus.
2017: ACG Case Reports Journal
https://www.readbyqxmd.com/read/28573178/endoscopic-esophagogastric-anastomosis-with-luminal-apposition-axios-stent-las-approach-a-new-concept-for-hybrid-lewis-santy
#3
Adrian Culetto, Jean-Michel Gonzalez, Geoffroy Vanbiervliet, Pablo Mira da Garcia, Juan Ignacio Tellechea, Emmanuelle Garnier, Stephane Berdah, Marc Barthet
BACKGROUND AND STUDY AIMS:  Esophagogastric anastomosis (EGA) has a high risk of leakage. Based upon our experience in endoscopic gastrojejunal anastomosis using LAS, the aim of this study was to verify the technical feasibility and the safety of performing an EGA using a hybrid approach (endoscopic and surgical). MATERIALS AND METHODS:  A pilot prospective study was performed on 8 survival pigs. The procedure was carried out in 2 stages: (i) surgical step consisting of an esogastrectomy by laparotomy with separated suture of the esophagus and stomach; (ii) endoscopic esophagogastric anastomosis using the LAS...
June 2017: Endoscopy International Open
https://www.readbyqxmd.com/read/28337480/intra-channel-stent-release-technique-for-fluoroless-endoscopic-ultrasound-guided-lumen-apposing-metal-stent-placement-changing-the-paradigm
#4
Andrea Anderloni, Fabia Attili, Silvia Carrara, Domenico Galasso, Milena Di Leo, Guido Costamagna, Alessandro Repici, Rastislav Kunda, Alberto Larghi
Background Recently, a novel lumen-apposing fully covered self-expanding metal stent (LA-FCSEMS) mounted on an electrocautery-enhanced delivery system has been developed to perform endoscopic ultrasound (EUS)-guided transluminal drainage. From early experience, however, release of the proximal flange of the stent has mostly been done using endoscopic view guidance to ensure proper positioning. Aim We describe a new technique that we have named the Intra-Channel Stent Release Technique (ICSRT) to perform stent placement under complete EUS control, without the use of either fluoroscopic or endoscopic views...
January 2017: Endoscopy International Open
https://www.readbyqxmd.com/read/28073590/objective-detection-of-apoptosis-in-rat-renal-tissue-sections-using-light-microscopy-and-free-image-analysis-software-with-subsequent-machine-learning-detection-of-apoptosis-in-renal-tissue
#5
Nayana Damiani Macedo, Aline Rodrigues Buzin, Isabela Bastos Binotti Abreu de Araujo, Breno Valentim Nogueira, Tadeu Uggere de Andrade, Denise Coutinho Endringer, Dominik Lenz
OBJECTIVE: The current study proposes an automated machine learning approach for the quantification of cells in cell death pathways according to DNA fragmentation. METHODS: A total of 17 images of kidney histological slide samples from male Wistar rats were used. The slides were photographed using an Axio Zeiss Vert.A1 microscope with a 40x objective lens coupled with an Axio Cam MRC Zeiss camera and Zen 2012 software. The images were analyzed using CellProfiler (version 2...
February 2017: Tissue & Cell
https://www.readbyqxmd.com/read/27847424/the-effect-of-lead-on-the-process-of-spermatogenesis-in-sex-glands-of-male-albino-rats
#6
Olga Sergeevna Shubina, Natalia Anatolievna Dudenkova
AIM: The aim of this study was to investigate the morphological and functional features of the process of spermatogenesis in the testes of male albino rats when exposed to lead acetate. MATERIALS AND METHODS: Using histological, morphometric and statistical methods explored the effects of lead acetate on the process of spermatogenesis in the testes of male albino rats. Investigations were carried out using a digital microscope Axio Imager. M2 and automatic cell counter Countess™...
October 2016: Veterinary World
https://www.readbyqxmd.com/read/27737366/marginal-and-internal-fit-of-zirconia-copings-obtained-using-different-digital-scanning-methods
#7
Lorena Oliveira Pedroche, Sérgio Rocha Bernardes, Moira Pedroso Leão, Cibele Cândida de Almeida Kintopp, Gisele Maria Correr, Bárbara Pick Ornaghi, Carla Castiglia Gonzaga
The objective of this study was to evaluate the marginal and internal fit of zirconia copings obtained with different digital scanning methods. A human mandibular first molar was set in a typodont with its adjacent and antagonist teeth and prepared for an all-ceramic crown. Digital impressions were made using an intraoral scanner (3Shape). Polyvinyl siloxane impressions and Type IV gypsum models were also obtained and scanned with a benchtop laboratory scanner (3Shape D700). Ten zirconia copings were fabricated for each group using CAD-CAM technology...
October 10, 2016: Brazilian Oral Research
https://www.readbyqxmd.com/read/27545192/evaluation-of-the-axios-stent-for-the-treatment-of-pancreatic-fluid-collections
#8
REVIEW
Eduardo Rodrigues-Pinto, Todd H Baron
INTRODUCTION: Endoscopic ultrasound guided translumenal drainage of pancreatic fluid collections (PFCs) has been widely practiced for over a decade, using conventional plastic and self-expandable metal stents. The use of such stents for transmural drainage is off-label and limited by the lack of lumen-to-lumen anchorage, which can lead to leakage, migration and tissue trauma. AREAS COVERED: Novel stent designs dedicated to applications of translumenal drainage have recently emerged and promise to make transmural drainage quicker, safer, and more effective...
September 2016: Expert Review of Medical Devices
https://www.readbyqxmd.com/read/27489992/single-step-endoscopic-ultrasound-guided-fluoroless-gallbladder-drainage-using-the-axios-lumen-apposing-metal-stent
#9
Carmelo Barbera, Giuseppe Grande, Nadia Alberghina, Mauro Manno, Rita Luisa Conigliaro
No abstract text is available yet for this article.
2016: Endoscopy
https://www.readbyqxmd.com/read/27332885/new-material-of-the-hominoid-ouranopithecus-macedoniensis-from-the-late-miocene-of-the-axios-valley-macedonia-greece-with-some-remarks-on-its-sexual-dimorphism
#10
George D Koufos, Louis de Bonis, Dimitris Kugiumtzis
New hominoid teeth from the late Miocene locality Ravin de la Pluie (RPl) of the Axios Valley (Macedonia, Greece) are studied in this article. Their morphology, dimensions and proportions are similar to the hominoid Ouranopithecus macedoniensis, allowing their attribution to this taxon. The studied material provides some new morphological characters for the female P3 (small asymmetry, small mesiobuccal crown projection, paracone higher than protocone) and the lower canine (mesial groove: large in the male and small in the female, distobuccal fovea: large in the female and small in the male)...
2016: Folia Primatologica; International Journal of Primatology
https://www.readbyqxmd.com/read/27189914/endoscopic-therapy-with-lumen-apposing-metal-stents-is-safe-and-effective-for-patients-with-pancreatic-walled-off-necrosis
#11
Reem Z Sharaiha, Amy Tyberg, Mouen A Khashab, Nikhil A Kumta, Kunal Karia, Jose Nieto, Uzma D Siddiqui, Irving Waxman, Virendra Joshi, Petros C Benias, Peter Darwin, Christopher J DiMaio, Christopher J Mulder, Shai Friedland, David G Forcione, Divyesh V Sejpal, Tamas A Gonda, Frank G Gress, Monica Gaidhane, Ann Koons, Ersilia M DeFilippis, Sanjay Salgado, Kristen R Weaver, John M Poneros, Amrita Sethi, Sammy Ho, Vivek Kumbhari, Vikesh K Singh, Alan H Tieu, Viviana Parra, Alisa Likhitsup, Craig Womeldorph, Brenna Casey, Sreeni S Jonnalagadda, Amit P Desai, David L Carr-Locke, Michel Kahaleh, Ali A Siddiqui
BACKGROUND & AIMS: Endoscopic ultrasound-guided transmural drainage and necrosectomy have become the standard treatment for patients with pancreatic walled-off necrosis (WON). Lumen-apposing metal stents (LAMS) have shown success in the management of pancreatic fluid collections. However, there are few data on their specific roles in management of WON. We investigated the efficacy and safety of LAMS in treatment of WON. METHODS: We performed a retrospective multicenter case series of 124 patients with WON who underwent endoscopic transmural drainage by using LAMS at 17 tertiary care centers from January 2014 through May 2015...
December 2016: Clinical Gastroenterology and Hepatology
https://www.readbyqxmd.com/read/27174406/in%C3%A2-vitro-assessment-of-the-marginal-and-internal-fits-of-interim-implant-restorations-fabricated-with-different-methods
#12
Jin-Young Park, Il-Do Jeong, Jae-Jun Lee, So-Yeon Bae, Ji-Hwan Kim, Woong-Chul Kim
STATEMENT OF PROBLEM: The fit of an interim implant restoration (IIR) is important for the effective treatment of patients with partial edentulism. However, no clinical trials have evaluated the marginal and internal fittings achieved with various fabrication methods. PURPOSE: The purpose of this in vitro study was to evaluate and compare the marginal and internal discrepancies in IIRs produced with 3 different methods. MATERIAL AND METHODS: Partially edentulous maxillary and mandibular casts from a transfer abutment were used...
October 2016: Journal of Prosthetic Dentistry
https://www.readbyqxmd.com/read/27065729/endoscopic-ultrasound-guided-placement-of-axios-stent-for-drainage-of-pancreatic-fluid-collections
#13
REVIEW
Rashmee Patil, Mel A Ona, Charilaos Papafragkakis, Sury Anand, Sushil Duddempudi
Pancreatic fluid collections (PFCs) have conventionally been treated with surgery, percutaneous drainage, or with the more recently established endoscopic ultrasound (EUS)-guided drainage modality. Currently, endoscopic plastic or metallic stents are used for PFC drainage. Plastic stents present issues with stent migration and premature occlusion requiring frequent stent exchanges or placement of additional stents. Metallic stents are tubular and may migrate, resulting in inefficient drainage, content leakage, retrieval and replacement, and possible mucosal injury...
April 2016: Annals of Gastroenterology: Quarterly Publication of the Hellenic Society of Gastroenterology
https://www.readbyqxmd.com/read/27061628/comparison-of-marginal-and-internal-fit-of-3-unit-ceramic-fixed-dental-prostheses-made-with-either-a-conventional-or-digital-impression
#14
Ting-Shu Su, Jian Sun
STATEMENT OF PROBLEM: For 20 years, the intraoral digital impression technique has been applied to the fabrication of computer aided design and computer aided manufacturing (CAD-CAM) fixed dental prostheses (FDPs). Clinical fit is one of the main determinants of the success of an FDP. Studies of the clinical fit of 3-unit ceramic FDPs made by means of a conventional impression versus a digital impression technology are limited. PURPOSE: The purpose of this in vitro study was to evaluate and compare the internal fit and marginal fit of CAD-CAM, 3-unit ceramic FDP frameworks fabricated from an intraoral digital impression and a conventional impression...
September 2016: Journal of Prosthetic Dentistry
https://www.readbyqxmd.com/read/26969661/eus-guided-choledochoduodenostomy-for-malignant-distal-biliary-obstruction-using-a-lumen-apposing-fully-covered-metal-stent-after-failed-ercp
#15
Rastislav Kunda, Manuel Pérez-Miranda, Uwe Will, Sebastian Ullrich, Dirk Brenke, Markus Dollhopf, Michelle Meier, Alberto Larghi
BACKGROUND: A novel lumen-apposing, self-expanding metal stent to perform EUS-guided drainage procedures has been recently developed. The aim of this study was to analyze the safety, technical and clinical effectiveness of this device for EUS-guided choledochoduodenostomy (EUS-CD) with palliative intent. METHODS: Retrospective analysis of all consecutive patients with unresectable malignant distal bile duct obstruction who, between March 2012 and September 2014, underwent EUS-CD using the study devices (AXIOS™ and Hot AXIOS™, Xlumena Inc...
March 11, 2016: Surgical Endoscopy
https://www.readbyqxmd.com/read/26456692/management-of-walled-off-necrosis-using-the-multiple-transluminal-gateway-technique-with-the-hot-axios-system
#16
LETTER
Ji Young Bang, Shyam Varadarajulu
No abstract text is available yet for this article.
January 2016: Digestive Endoscopy: Official Journal of the Japan Gastroenterological Endoscopy Society
https://www.readbyqxmd.com/read/26397845/treatment-of-a-benign-anastomotic-refractory-rectal-stricture-with-an-axios-stent
#17
Felipe Martínez Alcalá, Felipe R Martínez-Alcalá García, Andres Sánchez-Yague, Alvaro Martínez-Alcalá García, Jose Antonio Ciria Avila, Jose Manuel Perez Pozo
No abstract text is available yet for this article.
2015: Endoscopy
https://www.readbyqxmd.com/read/26391151/cd68-but-not-stabilin-1-tumor-associated-macrophages-in-gaps-of-ductal-tumor-structures-negatively-correlate-with-the-lymphatic-metastasis-in-human-breast-cancer
#18
Mikhail Buldakov, Marina Zavyalova, Nadezhda Krakhmal, Nadezhda Telegina, Sergei Vtorushin, Irina Mitrofanova, Vladimir Riabov, Shuiping Yin, Bin Song, Nadezhda Cherdyntseva, Julia Kzhyshkowska
Tumor associated macrophages (TAM) support tumor growth and metastasis in several animal models of breast cancer, and TAM amount is predictive for efficient tumor growth and metastatic spread via blood circulation. However, limited information is available about intratumoral TAM heterogeneity and functional role of TAM subpopulations in tumor progression. The aim of our study was to examine correlation of TAM presence in various morphological segments of human breast cancer with clinical parameters. Thirty six female patients with nonspecific invasive breast cancer T1-4N0-3M0 were included in the study...
January 2017: Immunobiology
https://www.readbyqxmd.com/read/26384400/automated-high-throughput-fluorescence-lifetime-imaging-microscopy-to-detect-protein-protein-interactions
#19
Camilo Guzmán, Christina Oetken-Lindholm, Daniel Abankwa
Fluorescence resonance energy transfer (FRET) is widely used to study conformational changes of macromolecules and protein-protein, protein-nucleic acid, and protein-small molecule interactions. FRET biosensors can serve as valuable secondary assays in drug discovery and for target validation in mammalian cells. Fluorescence lifetime imaging microscopy (FLIM) allows precise quantification of the FRET efficiency in intact cells, as FLIM is independent of fluorophore concentration, detection efficiency, and fluorescence intensity...
April 2016: Journal of Laboratory Automation
https://www.readbyqxmd.com/read/26231846/the-first-record-of-a-soft-shelled-turtle-testudines-pan-trionychidae-from-southern-balkans-pliocene-gefira-n-greece-and-new-information-from-bone-histology
#20
Evangelos Vlachos, Ignacio Cerda, Evangelia Tsoukala
Soft-shelled turtles (Pan-Trionychidae) are not included in the present-day chelonian fauna of Greece and have been unknown in the Greek fossil record up to now. Here, we report the first fossil occurrence of a soft-shelled turtle from Greece, originating from the Pliocene Gefira Member (Angelochori Formation), in the lower Axios valley. The corresponding specimens were discovered with several mammalian remains, most of them attributable to the mastodon of Auvergne, Anancus arvernensis. The chelonian material includes five carapacial fragments that belong to the same individual and can be attributed to Pan-Trionychidae based on the typical sculpturing on the dorsal side of the carapace...
August 2015: Die Naturwissenschaften
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