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https://www.readbyqxmd.com/read/29346060/a-combination-with-probiotic-complex-zinc-and-coenzyme-q10-attenuates-autoimmune-arthritis-by-regulation-of-th17-treg-balance
#1
Seon-Young Lee, Seung Hoon Lee, JooYeon Jhun, Hyeon-Beom Seo, Kyung Ah Jung, Chul Woo Yang, Sung-Hwan Park, Mi-La Cho
Probiotic complex, zinc, and coenzyme Q10 (CoQ10) are recognized dietary supplements with an anti-inflammatory role. Although these supplementations are individually known to benefit rheumatoid arthritis (RA), there is no evidence suggesting any synergic effect. The primary goal of this study is to determine whether probiotic complex, zinc, and CoQ10 attenuate the development of collagen-induced arthritis (CIA). The combination of probiotic complex, zinc, and CoQ10 reduced CIA severity by downregulating the levels of IgG, IgG1, and IgG2a in serum...
January 2018: Journal of Medicinal Food
https://www.readbyqxmd.com/read/29346012/the-combination-of-probiotic-complex-rosavin-and-zinc-improves-pain-and-cartilage-destruction-in-an-osteoarthritis-rat-model
#2
Ji Ye Kwon, Seung Hoon Lee, JooYeon Jhun, JeongWon Choi, KyungAh Jung, Keun Hyung Cho, Seok Jung Kim, Chul Woo Yang, Sung-Hwan Park, Mi-La Cho
Osteoarthritis (OA), a degenerative disorder, induces pain, joint inflammation, and destruction of the articular cartilage matrix. Probiotic complex, rosavin, and zinc have been used as dietary supplements that exhibit anti-inflammatory and antioxidant properties. However, there is no evidence demonstrating a synergic effect in OA. This study aims to determine whether combination with probiotic complex, rosavin, and zinc decreases progression of monosodium iodoacetate (MIA)-induced OA rat model. The combination improved pain levels by preventing cartilage damage...
January 18, 2018: Journal of Medicinal Food
https://www.readbyqxmd.com/read/29345071/detecting-selection-signatures-on-the-x-chromosome-of-the-chinese-debao-pony
#3
X-X Liu, J-F Pan, Q-J Zhao, X-H He, Y-B Pu, J-L Han, Y-H Ma, L Jiang
The X chromosome shows a special interaction between demographic factors and genetic variation, and the analysis of X-linked genomic variation can therefore provide insights into the unique effects of demography and selection on the horse genome that cannot be readily detected by autosomal markers. Debao (DB) ponies have experienced intense selective pressure for the development of their small stature (<106 cm at adult height). To identify selective sweeps on the X chromosome of the DB pony, we performed a genome-wide scan of three Chinese horse breeds using an Equine SNP70 BeadChip...
February 2018: Journal of Animal Breeding and Genetics, Zeitschrift Für Tierzüchtung und Züchtungsbiologie
https://www.readbyqxmd.com/read/29344873/laser-capture-microdissection-and-rna-extraction-from-perfusion-fixed-cartilage-and-bone-tissue-from-mice-implanted-with-human-ipsc-derived-mscs-in-a-calvarial-defect-model
#4
Xiaonan Xin, Xi Jiang, Alexander Lichtler, Mark Kronenberg, David Rowe, Joel S Pachter
Laser-capture microdissection (LCM) coupled to downstream RNA analysis poses unique difficulties for the evaluation of mineralized tissues. A rapid protocol was thus developed to enable sufficient integrity of bone and cartilage tissue for reliable sectioning, while minimizing RNA loss associated with prolonged decalcification and purification steps. Specifically, the protocol involves pump-assisted, cardiac perfusion-fixation with paraformaldehyde, and moderate digestion of LCM-acquired tissue with proteinase K followed by DNase treatment and separation of RNA using magnetic beads...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29344807/inhibition-of-bone-resorption-by-bisphosphonates-interferes-with-orthodontically-induced-midpalatal-suture-expansion-in-mice
#5
Till Koehne, Bärbel Kahl-Nieke, Michael Amling, Heike Korbmacher-Steiner
OBJECTIVES: Craniofacial sutures are important growth sites for skull development and are sensitive to mechanical stress. In order to determine the role of bone resorption in stress-mediated sutural bone growth, midpalatal suture expansion was performed in mice receiving alendronate, an anti-resorptive bisphosphonate. MATERIALS AND METHODS: The midpalatal sutures of 8-week-old C57BL/6 mice were expanded by orthodontic wires over the period of 2 weeks. Mice with maxillary expansion without drug treatment as well as untreated animals served as controls...
January 18, 2018: Clinical Oral Investigations
https://www.readbyqxmd.com/read/29343853/differential-tissue-specific-temporal-and-spatial-expression-patterns-of-the-aggrecan-gene-is-modulated-by-independent-enhancer-elements
#6
Ian M H Li, Ke Liu, Alice Neal, Peter D Clegg, Sarah De Val, George Bou-Gharios
The transcriptional mechanism through which chondrocytes control the spatial and temporal composition of the cartilage tissue has remained largely elusive. The central aim of this study was to identify whether transcriptional enhancers played a role in the organisation of the chondrocytes in cartilaginous tissue. We focused on the Aggrecan gene (Acan) as it is essential for the normal structure and function of cartilage and it is expressed developmentally in different stages of chondrocyte maturation. Using transgenic reporter studies in mice we identified four elements, two of which showed individual chondrocyte developmental stage specificity...
January 17, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29343182/fibronectin-enhances-cartilage-repair-by-activating-progenitor-cells-through-integrin-%C3%A9-5%C3%AE-1-receptor
#7
Tianqi Tao, Yang Li, Chang Gui, Yong Ma, Yingbin Ge, Hanhao Dai, Kaibin Zhang, Jing Du, Yang Guo, Yiqiu Jiang, Jianchao Gui
This study aimed to determine the effect of fibronectin (FN) on cartilage regeneration through the activation of chondrogenic progenitor cells (CPCs). Cells were isolated from the knee cartilage of mice and cultured in the presence of various concentrations of FN. Proliferation, migration and chondrogenic differentiation assays were performed in vitro. In some experiments, CPCs were pre-incubated with anti-integrin ɑ5β1 antibody for 60 min prior to FN treatment to block the integrin ɑ5β1 receptor. Soluble FN was mixed with Pluronic F-127 and injected into the joint cavity in an early-stage osteoarthritis (OA) model...
January 18, 2018: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/29342965/lipid-based-nanoparticles-as-a-potential-delivery-approach-in-the-treatment-of-rheumatoid-arthritis
#8
REVIEW
Shih-Yi Chuang, Chih-Hung Lin, Tse-Hung Huang, Jia-You Fang
Rheumatoid arthritis (RA), a chronic and joint-related autoimmune disease, results in immune dysfunction and destruction of joints and cartilages. Small molecules and biological therapies have been applied in a wide variety of inflammatory disorders, but their utility as a therapeutic agent is limited by poor absorption, rapid metabolism, and serious side effects. To improve these limitations, nanoparticles, which are capable of encapsulating and protecting drugs from degradation before they reach the target site in vivo, may serve as drug delivery systems...
January 15, 2018: Nanomaterials
https://www.readbyqxmd.com/read/29342370/fgf8-signaling-alters-the-osteogenic-cell-fate-in-the-hard-palate
#9
J Xu, Z Huang, W Wang, X Tan, H Li, Y Zhang, W Tian, T Hu, Y P Chen
Fibroblast growth factor (FGF) signaling has been implicated in the regulation of osteogenesis in both intramembranous and endochondral ossifications. In the developing palate, the anterior bony palate forms by direct differentiation of cranial neural crest (CNC)-derived mesenchymal cells, but the signals that regulate the osteogenic cell fate in the developing palate remain unclear. In the present study, we investigated the potential role of FGF signaling in osteogenic fate determination of the palatal mesenchymal cells...
January 1, 2018: Journal of Dental Research
https://www.readbyqxmd.com/read/29340978/methotrexate-aspasomes-against-rheumatoid-arthritis-optimized-hydrogel-loaded-liposomal-formulation-with-in-vivo-evaluation-in-wistar-rats
#10
Saikat Ghosh, Biswajit Mukherjee, Shreyasi Chaudhuri, Tanushree Roy, Alankar Mukherjee, Soma Sengupta
Aspasomes of methotrexate with antioxidant, ascorbyl palmitate, were developed and optimized using factorial design by varying parameters such as lipid molar ratio, drug to lipid molar ratio, and type of hydration buffer for transdermal delivery for disease modifying activity in rheumatoid arthritis (RA). Aspasomes were characterized by drug-excipients interaction, particle size analysis, determination of zeta potential, entrapment efficiency, and surface properties. The best formulation was loaded into hydrogel for evaluation of in vitro drug release and tested in vivo against adjuvant induced arthritis model in wistar rats, by assessing various physiological, biochemical, hematological, and histopathological parameters...
January 16, 2018: AAPS PharmSciTech
https://www.readbyqxmd.com/read/29339093/the-proteoglycan-biglycan-mediates-inflammatory-response-by-activating-tlr-4-in-human-chondrocytes-inhibition-by-specific-sirna-and-high-polymerized-hyaluronan
#11
Angela Avenoso, Angela D'Ascola, Michele Scuruchi, Giuseppe Mandraffino, Alberto Calatroni, Antonino Saitta, Salvatore Campo, Giuseppe M Campo
Cartilage degeneration are hallmarks of wear, tear, mechanical and inflammatory damage of the joint cartilage. Tissue degradation as well as compromising the integrity and function of the organ, produces different intermediates, directly able to stimulate further inflammatory effect, therefore, amplifying the inflammation response. Biglycan is a soluble component of the extracellular matrix that is released during tissue injury. It has been reported that released biglycan is an endogenous ligand for TLR-2/4 in some cell type...
January 12, 2018: Archives of Biochemistry and Biophysics
https://www.readbyqxmd.com/read/29339024/nrf2-are-pathway-attenuates-oxidative-and-apoptotic-response-in-human-osteoarthritis-chondrocytes-by-activating-erk1-2-elk1-p70s6k-p90rsk-signaling-axis
#12
Nazir M Khan, Imran Ahmad, Tariq M Haqqi
Nrf2, a redox regulated transcription factor, has recently been shown to play a role in cartilage integrity but the mechanism remains largely unknown. Osteoarthritis (OA) is a multifactorial disease in which focal degradation of cartilage occurs. Here, we studied whether Nrf2 exerts chondroprotective effects by suppressing the oxidative stress and apoptosis in IL-1β stimulated human OA chondrocytes. Expression of Nrf2 and its target genes HO-1, NQO1 and SOD2 was significantly high in OA cartilage compared to normal cartilage and was also higher in damaged area compared to smooth area of OA cartilage of the same patient...
January 12, 2018: Free Radical Biology & Medicine
https://www.readbyqxmd.com/read/29337200/mimicking-the-3d-biology-of-osteochondral-tissue-with-microfluidic-based-solutions-breakthroughs-towards-boosting-drug-testing-and-discovery
#13
REVIEW
Mariana R Carvalho, Rui Luís Reis, Joaquim Miguel Oliveira
The development of tissue-engineering (TE) solutions for osteochondral (OC) regeneration has been slowed by technical hurdles related to the recapitulation of their complex and hierarchical architecture. OC defects refer to damage of both the articular cartilage and the underlying subchondral bone. To repair an OC tissue defect, the complexity of the bone and cartilage must be considered. To help achieve this, microfluidics is converging with TE approaches to provide new treatment possibilities. Microfluidics uses precise micrometer-to-millimeter-scale fluid flows to achieve high-resolution and spatial and/or temporal control of the cell microenvironment, providing powerful tools for cell culturing...
January 11, 2018: Drug Discovery Today
https://www.readbyqxmd.com/read/29334399/chondrocyte-burst-promotes-space-for-mineral-expansion
#14
Emilio Satoshi Hara, Masahiro Okada, Noriyuki Nagaoka, Takako Hattori, Letycia Mary Iida, Takuo Kuboki, Takayoshi Nakano, Takuya Matsumoto
Analysis of tissue development from multidisciplinary approaches can result in more integrative biological findings, and can eventually allow the development of more effective bioengineering methods. In this study, we analyzed the initial steps of mineral formation during secondary ossification of mouse femur based on biological and bioengineering approaches. We first found that some chondrocytes burst near the mineralized area. External factors that could trigger chondrocyte burst were then investigated. Chondrocyte burst was shown to be modulated by mechanical and osmotic pressure...
January 15, 2018: Integrative Biology: Quantitative Biosciences From Nano to Macro
https://www.readbyqxmd.com/read/29332174/a-friend-in-knee-ccn3-may-inhibit-osteoarthritis-progression
#15
Alex Peidl
Osteoarthritis (OA) is a major clinical problem among the ageing population, yet no disease-modifying treatments currently exist. This issue arises, in part, due to the complex processes occurring in the microenvironment of articular cartilage that lead to osteoarthritic changes. Gaining a better understanding of these processes is crucial in developing a viable therapy for OA. A recent report in Journal of Bone Mineral Metabolism by Janune et al. (J Bone Miner Metab 35:582-597, 2016) suggests a novel role for CCN3 in maintaining the differentiated phenotype of articular cartilage...
January 13, 2018: Journal of Cell Communication and Signaling
https://www.readbyqxmd.com/read/29331090/enhanced-chondrogenesis-of-mesenchymal-stem-cells-over-silk-fibroin-chitosan-chondroitin-sulfate-three-dimensional-scaffold-in-dynamic-culture-condition
#16
Parinita Agrawal, Krishna Pramanik, Varshini Vishwanath, Amit Biswas, Akalabya Bissoyi, Pradeep Kumar Patra
Chondroitin sulfate (Ch) is one of the main structural components of cartilage tissue, therefore, its presence in tissue engineered scaffold is expected to enhance cartilage regeneration. Previously, silk fibroin/chitosan (SF/CS) blend was proven to be a potential biomaterial for tissue development. In this study, the effect of Ch on physicochemical and biological properties of SF/CS blend was investigated and scaffolds with 0.8 wt% Ch was found to be favorable. The scaffolds possess pore size of 37-212 µm, contact angle 46...
January 13, 2018: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
https://www.readbyqxmd.com/read/29330730/scaffold-free-tissue-engineering-for-injured-joint-surface-restoration
#17
REVIEW
Kazunori Shimomura, Wataru Ando, Hiromichi Fujie, David A Hart, Hideki Yoshikawa, Norimasa Nakamura
Articular cartilage does not heal spontaneously due to its limited healing capacity, and thus effective treatments for cartilage injuries has remained challenging. Since the first report by Brittberg et al. in 1994, autologous chondrocyte implantation (ACI) has been introduced into the clinic. Recently, as an alternative for chondrocyte-based therapy, mesenchymal stem cell (MSC)-based therapy has received considerable research attention because of the relative ease in handling for tissue harvest, and subsequent cell expansion and differentiation...
January 5, 2018: Journal of Experimental Orthopaedics
https://www.readbyqxmd.com/read/29329878/course-dose-and-stage-dependent-toxic-effects-of-prenatal-dexamethasone-exposure-on-fetal-articular-cartilage-development
#18
Ze Chen, Zhe Zhao, Yunzepeng Li, Xingyu Zhang, Bin Li, Liaobin Chen, Hui Wang
Dexamethasone, a synthetic long-acting glucocorticoid, is routinely used for treating mothers at risk for preterm delivery. However, intrauterine overexposure to glucocorticoids induces low birth weight and cartilage dysplasia in offspring. Also, the "critical window" and safe dose of this treatment are largely unknown. This study investigated the course-, dose-, and stage-dependent toxic effects and the possible mechanisms of prenatal dexamethasone exposure (PDE) on fetal development and articular cartilage development...
January 9, 2018: Toxicology Letters
https://www.readbyqxmd.com/read/29329496/positive-feedback-regulation-of-subchondral-h-type-vessel-formation-by-chondrocyte-promotes-osteoarthritis-development-in-mice
#19
Jiansen Lu, Haiyan Zhang, Daozhang Cai, Chun Zeng, Pinglin Lai, Yan Shao, Hang Fang, Delong Li, Jiayao Ouyang, Chang Zhao, Denghui Xie, Bin Huang, Jian Yang, Yu Jiang, Xiaochun Bai
Vascular-invasion-mediated interactions between activated articular chondrocytes and subchondral bone are essential for osteoarthritis (OA) development. Here, we determined the role of nutrient sensing mechanistic target of rapamycin complex 1 (mTORC1) signaling in the crosstalk across the bone cartilage interface and its regulatory mechanisms. Then mice with chondrocyte-specific mTORC1 activation (Tsc1 CKO and Tsc1 CKOER ) or inhibition (Raptor CKOER ) and their littermate controls were subjected to OA induced by destabilization of the medial meniscus (DMM) or not...
January 12, 2018: Journal of Bone and Mineral Research: the Official Journal of the American Society for Bone and Mineral Research
https://www.readbyqxmd.com/read/29329193/mustn1-a-developmentally-regulated-pan-musculoskeletal-cell-marker-and-regulatory-gene
#20
REVIEW
Michael Hadjiargyrou
The Mustn1 gene encodes a small nuclear protein (~9.6 kDa) that does not belong to any known family. Its genomic organization consists of three exons interspersed by two introns and it is highly homologous across vertebrate species. Promoter analyses revealed that its expression is regulated by the AP family of transcription factors, especially c-Fos, Fra-2 and JunD. Mustn1 is predominantly expressed in the major tissues of the musculoskeletal system: bone, cartilage, skeletal muscle and tendon. Its expression has been associated with normal embryonic development, postnatal growth, exercise, and regeneration of bone and skeletal muscle...
January 12, 2018: International Journal of Molecular Sciences
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