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Graf method

Eszter Vojcek, Márta Csécsei, Edina Flach, Gábor Rudas, Rózsa Gráf, Erzsébet Princzkel
Background and purpose: We aimed to analyze patient characteristics of term neonates with the diagnosis of stroke between 2006 and 2017 at the 3rd level Neonatal Intensive Care Unit of the Szent János Hospital. Methods: We conducted a retrospective and prospective analysis including 18 newborns with stroke. Presentation, imaging methods, etiology and clinical context were discussed. All patients had a follow-up at 2 years of age or later. Subject of the study - In the past 10 years 17 term born and one premature neonate born at 36 weeks of age were diagnosed with stroke in our unit...
March 30, 2018: Ideggyógyászati Szemle
Corné Swart, Silvia Martínez-Jaime, Michal Gorka, Kerstin Zander, Alexander Graf
In-gel digestion has been used as a standard method for the preparation of protein samples for mass spectrometry analysis for over 25 years. Traditional in gel-digestion procedures require extensive sample handling, are prone to contamination and not compatible with high-throughput sample preparation. To address these shortcomings, we have modified the conventional in-gel digestion procedure for high-throughput proteomics studies. The modified method, termed "High Throughput in Gel digestion" (HiT-Gel), is based on a 96-well plate format which results in a drastic reduction in labour intensity and sample handling...
June 5, 2018: Scientific Reports
Matthias Beuerle, Daniel Graf, Henry F Schurkus, Christian Ochsenfeld
We present efficient methods to calculate beyond random phase approximation (RPA) correlation energies for molecular systems with up to 500 atoms. To reduce the computational cost, we employ the resolution-of-the-identity and a double-Laplace transform of the non-interacting polarization propagator in conjunction with an atomic orbital formalism. Further improvements are achieved using integral screening and the introduction of Cholesky decomposed densities. Our methods are applicable to the dielectric matrix formalism of RPA including second-order screened exchange (RPA-SOSEX), the RPA electron-hole time-dependent Hartree-Fock (RPA-eh-TDHF) approximation, and RPA renormalized perturbation theory using an approximate exchange kernel (RPA-AXK)...
May 28, 2018: Journal of Chemical Physics
Svemir Čustović, Sahmir Šadić, Aleksandar Vujadinović, Asmir Hrustić, Mahir Jašarević, Amer Čustović, Ferid Krupić
Aim To assess the relationship between the clinical sign of limited hip abduction and developmental dysplasia of the hip (DDH). Methods A research was conducted on 450 newborns at the Neonatal Unit at the Clinic of Gynaecology and Obstetrics and the Orthopaedics and Traumatology Clinic of the University Clinical Centre, Tuzla, between 30th August 2011 and 30th April 2012. Clinical (degree of hip abduction) and ultrasound examination of all newborns' hips were performed using the Graf method on their first day of life...
August 1, 2018: Medicinski Glasnik
Hanna Thomsen, Fabrice E Graf, Anne Farewell, Marica B Ericson
One pertinent complication in bacterial infection is the growth of biofilms, i.e., communities of surface-adhered bacteria resilient to antibiotics. Photodynamic inactivation has been proposed as an alternative to antibiotic treatment; however, novel techniques complementing standard efficacy measures are required. Herein, we present an approach employing multiphoton microscopy complemented with Airyscan super-resolution microscopy, to visualize the distribution of curcumin in Staphylococcus epidermidis biofilms...
May 21, 2018: Journal of Biophotonics
Liang Xue, Zhong Ming Ma, Shao Ping DU
A split-field design experiment was carried out using two main methods of cultivation (grafting and self-rooted cultivation) and subplots with different nitrogen application levels (0, 120, 240, and 360 kg N·hm-2 ) to investigate the effects of cultivation method and nitrogen application levels on the yield and quality of melons, nitrogen transfer, nitrogen distribution, and nitrogen utilization rate. The results showed that melons produced by grafting cultivation had a 7.3% increase in yield and a 0.16%-3...
June 18, 2017: Ying Yong Sheng Tai Xue Bao, the Journal of Applied Ecology
Moreno Zolfo, Francesco Asnicar, Paolo Manghi, Edoardo Pasolli, Adrian Tett, Nicola Segata
BACKGROUND: The microbial communities populating human and natural environments have been extensively characterized with shotgun metagenomics, which provides an in-depth representation of the microbial diversity within a sample. Microbes thriving in urban environments may be crucially important for human health, but have received less attention than those of other environments. Ongoing efforts started to target urban microbiomes at a large scale, but the most recent computational methods to profile these metagenomes have never been applied in this context...
May 9, 2018: Biology Direct
Tim Kehl, Lara Schneider, Kathrin Kattler, Daniel Stöckel, Jenny Wegert, Nico Gerstner, Nicole Ludwig, Ute Distler, Markus Schick, Ulrich Keller, Stefan Tenzer, Manfred Gessler, Jörn Walter, Andreas Keller, Norbert Graf, Eckart Meese, Hans-Peter Lenhof
Motivation: Transcriptional regulators play a major role in most biological processes. Alterations in their activities are associated with a variety of diseases and in particular with tumor development and progression. Hence, it is important to assess the effects of deregulated regulators on pathological processes. Results: Here, we present REGGAE (REGulator-Gene Association Enrichment), a novel method for the identification of key transcriptional regulators that have a significant effect on the expression of a given set of genes, e...
May 7, 2018: Bioinformatics
Monica S Spychala, Venugopal Reddy Venna, Michal Jandzinski, Sarah J Doran, David J Durgan, Bhanu Priya Ganesh, Nadim J Ajami, Nagireddy Putluri, Joerg Graf, Robert M Bryan, Louise D McCullough
Objective Chronic systemic inflammation contributes to the pathogenesis of many age-related diseases. Although not well understood, alterations in the gut microbiota, or dysbiosis, may be responsible for age-related inflammation. Methods Using stroke as a disease model, we tested the hypothesis that a youthful microbiota, when established in aged mice, produces positive outcomes following ischemic stroke. Conversely, an aged microbiota, when established in young mice, produces negative outcomes after stroke...
May 7, 2018: Annals of Neurology
Eric L Shirley, Mitchell Furst, Uwe Arp
We have developed a new method for the exact calculation of synchrotron radiation for the National Institute of Standards and Technology Synchrotron Ultraviolet Radiation Facility, SURF III. Instead of using the Schwinger formula, which is only an approximation, we develop formulae based on Graf's addition theorem for Bessel functions and accurate asymptotic expansions for Hankel functions and Bessel functions. By measuring the radiation intensity profile at two distances from the storage ring, we also confirm an apparent vertical emittance that is consistent with the vertical betatron oscillations that are intentionally introduced to extend beam lifetime by spreading the electron beam spatially...
April 2018: Review of Scientific Instruments
Dornoosh Zonoobi, Abhilash Hareendranathan, Emanuel Mostofi, Myles Mabee, Saba Pasha, Dana Cobzas, Padma Rao, Sukhdeep K Dulai, Jeevesh Kapur, Jacob L Jaremko
Purpose To validate accuracy of diagnosis of developmental dysplasia of the hip (DDH) from geometric properties of acetabular shape extracted from three-dimensional (3D) ultrasonography (US). Materials and Methods In this retrospective multi-institutional study, 3D US was added to conventional two-dimensional (2D) US of 1728 infants (mean age, 67 days; age range, 3-238 days) evaluated for DDH from January 2013 to December 2016. Clinical diagnosis after more than 6 months follow-up was normal (n = 1347), borderline (Graf IIa, later normalizing spontaneously; n = 140) or dysplastic (Graf IIb or higher, n = 241)...
June 2018: Radiology
Osama Sabri, Philipp M Meyer, Susanne Gräf, Swen Hesse, Stephan Wilke, Georg-Alexander Becker, Michael Rullmann, Marianne Patt, Julia Luthardt, Gudrun Wagenknecht, Alexander Hoepping, Rene Smits, Annegret Franke, Bernhard Sattler, Solveig Tiepolt, Steffen Fischer, Winnie Deuther-Conrad, Ulrich Hegerl, Henryk Barthel, Peter Schönknecht, Peter Brust
In early Alzheimer's dementia, there is a need for PET biomarkers of disease progression with close associations to cognitive dysfunction that may aid to predict further cognitive decline and neurodegeneration. Amyloid biomarkers are not suitable for that purpose. The α4β2 nicotinic acetylcholine receptors (α4β2-nAChRs) are widely abundant in the human brain. As neuromodulators they play an important role in cognitive functions such as attention, learning and memory. Post-mortem studies reported lower expression of α4β2-nAChRs in more advanced Alzheimer's dementia...
June 1, 2018: Brain: a Journal of Neurology
Peter C Keil, Nicole Lindenberg, Christoph L Lassen, Bernhard M Graf, Jens Meier, Christoph H Wiese
BACKGROUND: Hypnosis is probably one of the oldest therapies known to man. In the last decades modern hypnosis has mainly been used by psychotherapists; however, hypnosis is becoming increasingly more important as a therapeutic method in medicine. Hypnosis can be used for a variety of medical indications. In the literature there is much evidence for the effectiveness of hypnosis. The aim of the present investigation was to demonstrate the effectiveness of hypnosis in inpatient treatment of chronic pain patients and to present a self-hypnosis program, which can be easily integrated into pain therapy...
June 2018: Der Schmerz
Daniel Graf, Matthias Beuerle, Henry F Schurkus, Arne Luenser, Gökcen Savasci, Christian Ochsenfeld
An efficient algorithm for calculating the random phase approximation (RPA) correlation energy is presented that is as accurate as the canonical molecular orbital resolution-of-the-identity RPA (RI-RPA) with the important advantage of an effective linear-scaling behavior (instead of quartic) for large systems due to a formulation in the local atomic orbital space. The high accuracy is achieved by utilizing optimized minimax integration schemes and the local Coulomb metric attenuated by the complementary error function for the RI approximation...
May 8, 2018: Journal of Chemical Theory and Computation
Eva C Graf, Ilka Ott, Julian Praceus, Felix Bourier, Tim C Lueth
PURPOSE: The minimally invasive closure of the left atrial appendage is a promising alternative to anticoagulation for stroke prevention in patients suffering from atrial fibrillation. One of the challenges of this procedure is the correct positioning and the coaxial alignment of the tip of the catheter sheath to the implant landing zone. METHOD: In this paper, a novel preoperative planning system is proposed that allows patient-individual shaping of catheters to facilitate the correct positioning of the catheter sheath by offering a patient-specific catheter shape...
June 2018: International Journal of Computer Assisted Radiology and Surgery
Po-Ling Chang, Michael Graf, Chao-Hsuan Hung, Aleksandra Radenovic
Here we present the realization of a novel fluorescence detection method based on the electromigration of fluorescent molecules within a nanocapillary combined with the laser excitation through a platinum (Pt)-coated nanocapillary. By using the Pt nanocapillary assisted focusing of a laser beam, we completely remove the background scattering on the tip of the electrophoretic nanocapillary. In this excitation geometry, we demonstrate a 1000-fold sensitivity enhancement (1.0 nM to 1.0 pM) compared to the detection in microcapillaries with epifluorescence illumination and fluorescence spectrophotometry...
May 9, 2018: Nano Letters
Cecilia N Pisoni, Sebastián A Muñoz, María N Tamborenea, Mercedes García, Ana Curti, Ana Cappuccio, Oscar Rillo, Patricia M Imamura, Emilce Schneeberger, Marcela Ballent, Mario L Cousseau, Jorge Velasco Zamora, Verónica Saurit, Sergio Toloza, María C Danielsen, Verónica I Bellomio, Cesar Graf, Sergio Paira, Javier Cavallasca, Bernardo Pons Estel, José L C Moreno, Mónica Díaz, Paula Alba, Marcela Verando, Guillermo Tate, Eduardo Mysler, Judith Sarano, Emma E Civit, Fabián Risueño, Pablo Álvarez Sepúlveda, María S Larroude, Marcos F Méndez, Andrea Conforti, Débora Sohn
OBJECTIVE: To study the prevalence and the associated factors of work disability (WD) in systemic lupus erythematosus (SLE) patients. METHODS: A sample of 419 SLE patients from an observational cross-sectional multicenter study was included. Sociodemographic features, disease characteristics, comorbidities, quality of life, unhealthy behaviors, and work-related factors were measured in a standardized interview. Work disability was defined by patient self-report of not being able to work because of SLE...
April 2, 2018: International Journal of Rheumatic Diseases
Hegger M Fritsch, Sabrina E M Almeida, Aguinaldo R Pinto, Tiago Gräf
CRF31_BC is an HIVs-1 recombinant form very prevalent in the southernmost capital city of Brazil, Porto Alegre. Recent studies have been describing a growing number of cases of infection by CRF31_BC in other Brazilian cities and countries, suggesting a process of expansion of this strain. Aiming to describe the city of origin, dispersion routes and demographic history of CRF31_BC, this study analyzed all HIV-1 CRF31_BC and Brazilian BC mosaic publicly available sequences. CRF31_BC classification was performed by bootscanning and tree reconstruction methods...
July 2018: Infection, Genetics and Evolution
Niels Graf, Anna Dichtl, Daniel Deimel, Dirk Sander, Heino Stöver
Background In Germany, drug use in sexual settings (i.e. chemsex) among men who have sex with men (MSM) has increasingly been the subject of discussion for considerable time. At the same time, however, little is known about this practice. It is against this background that what is currently known about chemsex among MSM in Germany is discussed. METHODS: The present study observations are essentially based on the results of two research projects of a qualitative-explorative nature. In-depth interviews with drug-using MSM (n=14), as well as qualitative, problem-centred interviews with drug-using MSM (n=75) and expert interviews (n=27) were conducted within these research projects...
April 2018: Sexual Health
Aline Padilha de Fraga, Tiago Gräf, Cleiton Schneider Pereira, Nilo Ikuta, André Salvador Kazantzi Fonseca, Vagner Ricardo Lunge
Avian infectious bronchitis virus (IBV) is the etiological agent of a highly contagious disease, which results in severe economic losses to the poultry industry. The spike protein (S1 subunit) is responsible for the molecular diversity of the virus and many sero/genotypes are described around the world. Recently a new standardized classification of the IBV molecular diversity was conducted, based on phylogenetic analysis of the S1 gene sequences sampled worldwide. Brazil is one of the biggest poultry producers in the world and the present study aimed to review the molecular diversity and reconstruct the evolutionary history of IBV in the country...
July 2018: Infection, Genetics and Evolution
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