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https://www.readbyqxmd.com/read/29216670/-systematic-review-of-the-burden-of-campylobacter-associated-disease
#1
Julia Lackner, Daniela Schlichting, Christine Müller-Graf, Matthias Greiner
Bacteria of the genus Campylobacter spp. are one of the most common causes of gastroenteritis and can lead to serious sequelae. Several studies have estimated the disease burden of Campylobacter spp. with the quantitative metric of disability-adjusted life years (DALY). The aim of this systematic review is to give an overview of the information available about different countries and periods for which DALYs were calculated and how the different results are comparable. One of the most important transmission pathways for Campylobacter spp...
December 7, 2017: Das Gesundheitswesen
https://www.readbyqxmd.com/read/29178886/physical-examination-in-undergraduate-medical-education-in-the-field-of-general-practice-a-scoping-review
#2
Dirk Moßhammer, Joachim Graf, Stefanie Joos, Rebekka Hertkorn
BACKGROUND: Physical examination (PE) is an essential clinical skill and a central part of a physician's daily activity. Teaching of PE has been integrated into medical school by many clinical disciplines with respective specific examination procedures. For instance, PE teaching in general practice may include a full-body examination approach. Studies show that PE-skills of medical students often need enhancement. The aim of this article was to scope the literature regarding the teaching and research of PE within general practice during undergraduate medical education...
November 25, 2017: BMC Medical Education
https://www.readbyqxmd.com/read/29173593/molecular-and-phylogenetic-analyses-of-salmonella-gallinarum-trace-the-origin-and-diversification-of-recent-outbreaks-of-fowl-typhoid-in-poultry-farms
#3
Silvia De Carli, Tiago Gräf, Diéssy Kipper, Fernanda Kieling Moreira Lehmann, Nathalie Zanetti, Franciele Maboni Siqueira, Samuel Cibulski, André Salvador Kazantzi Fonseca, Nilo Ikuta, Vagner Ricardo Lunge
Fowl typhoid (FT) and pullorum disease (PD) are two important poultry infections caused by Salmonella enterica subsp. enterica serotype Gallinarum (S. Gallinarum). S. Gallinarum strains are adapted to birds and classified into biovars Gallinarum (bvGA) and Pullorum (bvPU) as they are the causative agent of FT and PD, respectively. In Brazil, FT/PD outbreaks have been reported along the last 50 years, but there was a recent increase of FT field reports with the suspicion it could be due to virulence reversion of the attenuated live vaccine SG9R...
December 2017: Veterinary Microbiology
https://www.readbyqxmd.com/read/29145478/electrical-impedance-tomography-amplitudes-of-cardiac-related-impedance-changes-in-the-lung-are-highly-position-dependent
#4
Michael Graf, Thomas Riedel
BACKGROUND: Electrical impedance tomography (EIT) is used on the thorax to measure impedance changes due to the presence of air and blood in the lung. This experimental study was performed to investigate the effect of posture on cardiac and respiratory related impedance changes. METHODS: EIT measurements were performed on 14 healthy subjects in left-, right lateral, prone, supine and upright positions. Simultaneously, tidal volume was recorded with an ultrasonic flowmeter...
2017: PloS One
https://www.readbyqxmd.com/read/29125399/an-analysis-of-variables-affecting-the-duration-of-pavlik-harness-treatment-is-it-possible-to-predict-the-duration-of-treatment
#5
Ahmet Salduz, Mehmet Demirel, Turgut Akgül, Fuat Bilgili
BACKGROUND: Pavlik harness is the initial treatment for the infants with developmental dysplasia of the hip who have not yet begun walking. OBJECTIVES: The present study evaluates the variables which affect the duration of PH treatment for patients successfully treated. STUDY DESIGN: Cross-sectional study. METHODS: Pavlik harness was employed for 140 hips of 87 patients with developmental dysplasia of the hip in our institution between 2010 and 2013...
November 1, 2017: Prosthetics and Orthotics International
https://www.readbyqxmd.com/read/29104456/sports-and-hdl-quality-reflected-by-serum-amyloid-a-and-surfactant-protein-b
#6
Michael Sponder, Chantal Kopecky, Ioana-Alexandra Campean, Michael Emich, Monika Fritzer-Szekeres, Brigitte Litschauer, Senta Graf, Marcus D Säemann, Jeanette Strametz-Juranek
Background: The aim of this prospective study was to investigate the influence of long-term physical activity on HDL quality, reflected by serum amyloid A (SAA) and surfactant protein B (SPB). Methods and results: 109 healthy subjects were recruited, 98 completed the study. Participants perform within the calculated training pulse for 8 months. The performance gain was measured/quantified by bicycle stress tests at the beginning and end of the observation period. SAA and SPB were measured at baseline and after 4 and 8 months by ELISA...
2017: International Journal of Medical Sciences
https://www.readbyqxmd.com/read/29096123/in-vitro-validation-of-a-novel-mechanical-model-for-testing-the-anchorage-capacity-of-pedicle-screws-using-physiological-load-application
#7
Christian Liebsch, Julia Zimmermann, Nicolas Graf, Christoph Schilling, Hans-Joachim Wilke, Annette Kienle
Biomechanical in vitro tests analysing screw loosening often include high standard deviations caused by high variabilities in bone mineral density and pedicle geometry, whereas standardized mechanical models made of PU foam often do not integrate anatomical or physiological boundary conditions. The purpose of this study was to develop a most realistic mechanical model for the standardized and reproducible testing of pedicle screws regarding the resistance against screw loosening and the holding force as well as to validate this model by in vitro experiments...
October 25, 2017: Journal of the Mechanical Behavior of Biomedical Materials
https://www.readbyqxmd.com/read/29047909/fiber-integrated-spectroscopy-device-for-hot-alkali-vapor
#8
Josephine Gutekunst, Daniel Weller, Harald Kübler, Jan-Philipp Negel, Marwan Abdou Ahmed, Thomas Graf, Robert Löw
We introduce an all-glass, vacuum tight, fiber-integrated and alkali compatible spectroscopy device consisting of two conventional optical fibers spliced to each end of a capillary. This is mainly realized through a decentered splicing method allowing refilling of the capillary and controlling the vapor density inside. We analyze the light guidance of the setup through simulations and measurements of the transmission efficiency at different wavelengths and show that filling it with highly reactive alkali metals is possible, and that the vapor density can be controlled reliably...
July 20, 2017: Applied Optics
https://www.readbyqxmd.com/read/29037139/analysis-of-free-amino-acids-in-mammalian-brain-extracts
#9
A L Ksenofontov, A I Boyko, G V Mkrtchyan, V N Tashlitsky, A V Timofeeva, A V Graf, V I Bunik, L A Baratova
An optimized method for analysis of free amino acids using a modified lithium-citrate buffer system with a Hitachi L-8800 amino acid analyzer is described. It demonstrates clear advantages over the sodium-citrate buffer system commonly used for the analysis of protein hydrolysates. A sample pretreatment technique for amino acid analysis of brain extracts is also discussed. The focus has been placed on the possibility of quantitative determination of the reduced form of glutathione (GSH) with simultaneous analysis of all other amino acids in brain extracts...
October 2017: Biochemistry. Biokhimii︠a︡
https://www.readbyqxmd.com/read/29030579/effect-of-iodinated-contrast-media-on-renal-perfusion-a-randomized-comparison-study-in-pigs-using-quantitative-contrast-enhanced-ultrasound-ceus
#10
Philipp Lamby, Friedrich Jung, Stefanie Graf, Lotte Schellenberg, Johannes Falter, Natascha Platz-da-Silva, Stephan Schreml, Lukas Prantl, Ralf P Franke, Ernst M Jung
The administration of iodinated contrast media (CM) can cause microcirculatory disorder leading to acute renal dysfunction. In a prospective, randomized investigation two CM (Iodixanol vs Iopromide) were compared in 16 pigs. Each animal received 10 intra-aortal injections (5 ml Iodixanol or 4.32 ml Iopromide). Microcirculation was assessed using contrast-enhanced ultrasound (CEUS) directly on the kidney surface using time-to-peak (TTP) and blood-volume-analysis. Macroscopic observations were documented...
October 13, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29028175/the-information-content-of-discrete-functions-and-their-application-in-genetic-data-analysis
#11
Nikita A Sakhanenko, James Kunert-Graf, David J Galas
The complex of central problems in data analysis consists of three components: (1) detecting the dependence of variables using quantitative measures, (2) defining the significance of these dependence measures, and (3) inferring the functional relationships among dependent variables. We have argued previously that an information theory approach allows separation of the detection problem from the inference of functional form problem. We approach here the third component of inferring functional forms based on information encoded in the functions...
October 13, 2017: Journal of Computational Biology: a Journal of Computational Molecular Cell Biology
https://www.readbyqxmd.com/read/29020612/covering-the-stem-cell-explosion-at-the-2017-isscr-conference-in-boston
#12
Thomas Graf
The meeting covered a plethora of rapidly evolving approaches and areas, such as organoid cultures modeling tissues and organs; stem cell-specific metabolites revealing new signaling pathways; single-cell technologies discovering new cell types and exploring stem cell niche interactions; novel methods studying stem cells in aging and cancer; lineage-tracing experiments exploring cell plasticity of tissues before and after injury; epigenetic studies illuminating cell reprogramming; new protocols improving cells for regenerative purposes; and several other timely and exciting topics...
October 10, 2017: Stem Cell Reports
https://www.readbyqxmd.com/read/28972005/phenotypic-characterisation-of-eif2ak4-mutation-carriers-in-a-large-cohort-of-patients-diagnosed-clinically-with-pulmonary-arterial-hypertension
#13
Charaka Hadinnapola, Marta Bleda, Matthias Haimel, Nicholas Screaton, Andrew J Swift, Peter Dorfmüller, Stephen D Preston, Mark Southwood, Jules Hernandez-Sanchez, Jennifer Martin, Carmen Treacy, Katherine Yates, Harm Bogaard, Colin Church, Gerry Coghlan, Robin Condliffe, Paul A Corris, Simon R Gibbs, Barbara Girerd, Simon Holden, Marc Humbert, David G Kiely, Allan Lawrie, Rajiv D Machado, Robert MacKenzie Ross, Shahin Moledina, David Montani, Michael Newnham, Andrew J Peacock, Joanna Pepke-Zaba, Paula J Rayner-Matthews, Olga Shamardina, Florent Soubrier, Laura Southgate, Jay Suntharalingam, Mark R Toshner, Richard C Trembath, Anton Vonk Noordegraaf, Martin R Wilkins, Stephen J Wort, John Wharton, Stefan Gräf, Nicholas W Morrell
Background -Pulmonary arterial hypertension (PAH) is a rare disease with an emerging genetic basis. Heterozygous mutations in the gene encoding the bone morphogenetic protein receptor type 2 (BMPR2) are the commonest genetic cause of PAH, whereas biallelic mutations in the eukaryotic translation initiation factor 2 alpha kinase 4 gene (EIF2AK4) are described in pulmonary veno-occlusive disease and pulmonary capillary haemangiomatosis (PVOD/PCH). Here, we determined the frequency of these mutations and define the genotype-phenotype characteristics in a large cohort of patients diagnosed clinically with PAH...
September 28, 2017: Circulation
https://www.readbyqxmd.com/read/28906265/a-retrospective-analysis-of-the-clinical-effectiveness-of-supraclavicular-ultrasound-guided-brachiocephalic-vein-cannulations-in-preterm-infants
#14
Christian Breschan, Gudrun Graf, Robert Jost, Haro Stettner, Georg Feigl, Stefan Neuwersch, Christian Stadik, Markus Koestenberger, Rudolf Likar
BACKGROUND: The aim of this retrospective analysis was to evaluate the clinical effectiveness of the supraclavicular ultrasound-guided cannulation of the brachiocephalic vein in preterm infants. METHODS: The ultrasound probe was placed in the supraclavicular region so as to obtain the optimum sonographic long-axis view of the brachiocephalic vein. By using a strict in-plane approach the brachiocephalic vein was cannulated by advancing a 22- or 24-gauge iv cannula from lateral to medial under the long axis of the ultrasound probe under real-time ultrasound guidance into the vein...
September 13, 2017: Anesthesiology
https://www.readbyqxmd.com/read/28777274/a-long-term-follow-up-of-young-adults-with-idiopathic-clubfoot-does-foot-morphology-relate-to-pain
#15
Adam N Graf, Ken N Kuo, Nikhil T Kurapati, Joseph J Krzak, Sahar Hassani, Angela K Caudill, Ann Flanagan, Gerald F Harris, Peter A Smith
BACKGROUND: Individuals with clubfoot, treated in infancy with either the Ponseti method or comprehensive clubfoot release, often encounter pain as adults. Multiple studies have characterized residual deformity after Ponseti or surgical correction using physical exam, radiographs and pedobarography; however, the relationship between residual foot deformity and pain is not well defined. The purpose of the current study was 2-fold: (1) to evaluate the relationship between foot morphology and pain for young adults treated as infants for idiopathic clubfoot and (2) to describe and compare pedobarographic measures and outcome measures of pain and morphology among surgically treated, Ponseti treated, and typically developing feet...
August 2, 2017: Journal of Pediatric Orthopedics
https://www.readbyqxmd.com/read/28729666/expansion-of-human-midbrain-floor-plate-progenitors-from-induced-pluripotent-stem-cells-increases-dopaminergic-neuron-differentiation-potential
#16
Stefania Fedele, Ginetta Collo, Katharina Behr, Josef Bischofberger, Stephan Müller, Tilo Kunath, Klaus Christensen, Anna Lisa Gündner, Martin Graf, Ravi Jagasia, Verdon Taylor
Human induced pluripotent stem cells (hiPSCs) are invaluable to study developmental processes and disease mechanisms particularly in the brain. hiPSCs can be differentiated into mature and functional dopaminergic (DA) neurons. Having robust protocols for the generation of differentiated DA neurons from pluripotent cells is a prerequisite for the use of hiPSCs to study disease mechanisms, for drug discovery, and eventually for cell replacement therapy. Here, we describe a protocol for generating and expanding large numbers of homogeneous midbrain floor plate progenitors (mFPPs) that retain efficient DA neurogenic potential over multiple passages and can be cryobanked...
July 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28717864/diagnosing-developmental-dysplasia-of-the-hip-using-the-graf-ultrasound-method-risk-and-protective-factor-analysis-in-11-820-universally-screened-newborns
#17
Mohammad Schams, Rob Labruyère, Anne Zuse, Mikolaj Walensi
The essential role of ultrasound examinations in diagnosis and treatment of developmental dysplasia of the hip (DDH) is widely accepted while the weighting and correlation of protective factors and perinatal risk for DDH still give rise to debate. Our aim was to investigate the impact of single and twofold combined risk and protective factors on the newborns' hip maturity, assessed with the Graf ultrasound method. Therefore, data sets of 11,820 universally screened newborns were analyzed. Univariate and logistic regression analyses were performed to correlate risk and protective factors with mature or immature but appropriate for age and pathologic hip types...
September 2017: European Journal of Pediatrics
https://www.readbyqxmd.com/read/28694036/-priority-setting-and-rationing-of-pharmaceuticals-an-experimental-analysis-of-discussion-processes
#18
Ines Aumann, Svenja Litzkendorf, Kathrin Damm, J Matthias Graf von der Schulenburg
BACKGROUND/OBJECTIVE: In the face of rising expenditure among statutory sickness funds in Germany it is necessary to start a discussion about priority setting in the healthcare system. For a long time this issue has been avoided in healthcare debates. As a result, normative directives are still missing, which can lead to priority setting among healthcare providers in daily healthcare practice. Prioritization can be conducted at three different levels: at the government (macro), the institutional (meso), and the patient (micro) level...
August 2017: Zeitschrift Für Evidenz, Fortbildung und Qualität Im Gesundheitswesen
https://www.readbyqxmd.com/read/28659574/validation-of-weak-biological-effects-by-round-robin-experiments-cytotoxicity-biocompatibility-of-sio2-and-polymer-nanoparticles-in-hepg2-cells
#19
Lisa Landgraf, Daniel Nordmeyer, Peter Schmiel, Qi Gao, Sandra Ritz, Julia S Gebauer, Stefan Graß, Silvia Diabaté, Lennart Treuel, Christina Graf, Eckart Rühl, Katharina Landfester, Volker Mailänder, Carsten Weiss, Reinhard Zellner, Ingrid Hilger
All over the world, different types of nanomaterials with a diversified spectrum of applications are designed and developed, especially in the field of nanomedicine. The great variety of nanoparticles (NPs), in vitro test systems and cell lines led to a vast amount of publications with conflicting data. To identify the decisive principles of these variabilities, we conducted an intercomparison study of collaborating laboratories within the German DFG Priority Program SPP1313, using well-defined experimental parameters and well-characterized NPs...
June 28, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28616821/re-irradiation-after-gross-total-resection-of-recurrent-glioblastoma-spatial-pattern-of-recurrence-and-a-review-of-the-literature-as-a-basis-for-target-volume-definition
#20
Christoph Straube, Greeshma Elpula, Jens Gempt, Julia Gerhardt, Stefanie Bette, Claus Zimmer, Friederike Schmidt-Graf, Bernhard Meyer, Stephanie E Combs
BACKGROUND: Currently, patients with gross total resection (GTR) of recurrent glioblastoma (rGBM) undergo adjuvant chemotherapy or are followed up until progression. Re-irradiation, as one of the most effective treatments in macroscopic rGBM, is withheld in this situation, as uncertainties about the pattern of re-recurrence, the target volume, and also the efficacy of early re-irradiation after GTR exist. METHODS: Imaging and clinical data from 26 consecutive patients with GTR of rGBM were analyzed...
November 2017: Strahlentherapie und Onkologie: Organ der Deutschen Röntgengesellschaft ... [et Al]
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