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https://www.readbyqxmd.com/read/29030579/effect-of-iodinated-contrast-media-on-renal-perfusion-a-randomized-comparison-study-in-pigs-using-quantitative-contrast-enhanced-ultrasound-ceus
#1
Philipp Lamby, Friedrich Jung, Stefanie Graf, Lotte Schellenberg, Johannes Falter, Natascha Platz-da-Silva, Stephan Schreml, Lukas Prantl, Ralf P Franke, Ernst M Jung
The administration of iodinated contrast media (CM) can cause microcirculatory disorder leading to acute renal dysfunction. In a prospective, randomized investigation two CM (Iodixanol vs Iopromide) were compared in 16 pigs. Each animal received 10 intra-aortal injections (5 ml Iodixanol or 4.32 ml Iopromide). Microcirculation was assessed using contrast-enhanced ultrasound (CEUS) directly on the kidney surface using time-to-peak (TTP) and blood-volume-analysis. Macroscopic observations were documented...
October 13, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29028175/the-information-content-of-discrete-functions-and-their-application-in-genetic-data-analysis
#2
Nikita A Sakhanenko, James Kunert-Graf, David J Galas
The complex of central problems in data analysis consists of three components: (1) detecting the dependence of variables using quantitative measures, (2) defining the significance of these dependence measures, and (3) inferring the functional relationships among dependent variables. We have argued previously that an information theory approach allows separation of the detection problem from the inference of functional form problem. We approach here the third component of inferring functional forms based on information encoded in the functions...
October 13, 2017: Journal of Computational Biology: a Journal of Computational Molecular Cell Biology
https://www.readbyqxmd.com/read/29020612/covering-the-stem-cell-explosion-at-the-2017-isscr-conference-in-boston
#3
Thomas Graf
The meeting covered a plethora of rapidly evolving approaches and areas, such as organoid cultures modeling tissues and organs; stem cell-specific metabolites revealing new signaling pathways; single-cell technologies discovering new cell types and exploring stem cell niche interactions; novel methods studying stem cells in aging and cancer; lineage-tracing experiments exploring cell plasticity of tissues before and after injury; epigenetic studies illuminating cell reprogramming; new protocols improving cells for regenerative purposes; and several other timely and exciting topics...
October 10, 2017: Stem Cell Reports
https://www.readbyqxmd.com/read/28972005/phenotypic-characterisation-of-eif2ak4-mutation-carriers-in-a-large-cohort-of-patients-diagnosed-clinically-with-pulmonary-arterial-hypertension
#4
Charaka Hadinnapola, Marta Bleda, Matthias Haimel, Nicholas Screaton, Andrew J Swift, Peter Dorfmüller, Stephen D Preston, Mark Southwood, Jules Hernandez-Sanchez, Jennifer Martin, Carmen Treacy, Katherine Yates, Harm Bogaard, Colin Church, Gerry Coghlan, Robin Condliffe, Paul A Corris, Simon R Gibbs, Barbara Girerd, Simon Holden, Marc Humbert, David G Kiely, Allan Lawrie, Rajiv D Machado, Robert MacKenzie Ross, Shahin Moledina, David Montani, Michael Newnham, Andrew J Peacock, Joanna Pepke-Zaba, Paula J Rayner-Matthews, Olga Shamardina, Florent Soubrier, Laura Southgate, Jay Suntharalingam, Mark R Toshner, Richard C Trembath, Anton Vonk Noordegraaf, Martin R Wilkins, Stephen J Wort, John Wharton, Stefan Gräf, Nicholas W Morrell
Background -Pulmonary arterial hypertension (PAH) is a rare disease with an emerging genetic basis. Heterozygous mutations in the gene encoding the bone morphogenetic protein receptor type 2 (BMPR2) are the commonest genetic cause of PAH, whereas biallelic mutations in the eukaryotic translation initiation factor 2 alpha kinase 4 gene (EIF2AK4) are described in pulmonary veno-occlusive disease and pulmonary capillary haemangiomatosis (PVOD/PCH). Here, we determined the frequency of these mutations and define the genotype-phenotype characteristics in a large cohort of patients diagnosed clinically with PAH...
September 28, 2017: Circulation
https://www.readbyqxmd.com/read/28906265/a-retrospective-analysis-of-the-clinical-effectiveness-of-supraclavicular-ultrasound-guided-brachiocephalic-vein-cannulations-in-preterm-infants
#5
Christian Breschan, Gudrun Graf, Robert Jost, Haro Stettner, Georg Feigl, Stefan Neuwersch, Christian Stadik, Markus Koestenberger, Rudolf Likar
BACKGROUND: The aim of this retrospective analysis was to evaluate the clinical effectiveness of the supraclavicular ultrasound-guided cannulation of the brachiocephalic vein in preterm infants. METHODS: The ultrasound probe was placed in the supraclavicular region so as to obtain the optimum sonographic long-axis view of the brachiocephalic vein. By using a strict in-plane approach the brachiocephalic vein was cannulated by advancing a 22- or 24-gauge iv cannula from lateral to medial under the long axis of the ultrasound probe under real-time ultrasound guidance into the vein...
September 13, 2017: Anesthesiology
https://www.readbyqxmd.com/read/28777274/a-long-term-follow-up-of-young-adults-with-idiopathic-clubfoot-does-foot-morphology-relate-to-pain
#6
Adam N Graf, Ken N Kuo, Nikhil T Kurapati, Joseph J Krzak, Sahar Hassani, Angela K Caudill, Ann Flanagan, Gerald F Harris, Peter A Smith
BACKGROUND: Individuals with clubfoot, treated in infancy with either the Ponseti method or comprehensive clubfoot release, often encounter pain as adults. Multiple studies have characterized residual deformity after Ponseti or surgical correction using physical exam, radiographs and pedobarography; however, the relationship between residual foot deformity and pain is not well defined. The purpose of the current study was 2-fold: (1) to evaluate the relationship between foot morphology and pain for young adults treated as infants for idiopathic clubfoot and (2) to describe and compare pedobarographic measures and outcome measures of pain and morphology among surgically treated, Ponseti treated, and typically developing feet...
August 2, 2017: Journal of Pediatric Orthopedics
https://www.readbyqxmd.com/read/28729666/expansion-of-human-midbrain-floor-plate-progenitors-from-induced-pluripotent-stem-cells-increases-dopaminergic-neuron-differentiation-potential
#7
Stefania Fedele, Ginetta Collo, Katharina Behr, Josef Bischofberger, Stephan Müller, Tilo Kunath, Klaus Christensen, Anna Lisa Gündner, Martin Graf, Ravi Jagasia, Verdon Taylor
Human induced pluripotent stem cells (hiPSCs) are invaluable to study developmental processes and disease mechanisms particularly in the brain. hiPSCs can be differentiated into mature and functional dopaminergic (DA) neurons. Having robust protocols for the generation of differentiated DA neurons from pluripotent cells is a prerequisite for the use of hiPSCs to study disease mechanisms, for drug discovery, and eventually for cell replacement therapy. Here, we describe a protocol for generating and expanding large numbers of homogeneous midbrain floor plate progenitors (mFPPs) that retain efficient DA neurogenic potential over multiple passages and can be cryobanked...
July 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28717864/diagnosing-developmental-dysplasia-of-the-hip-using-the-graf-ultrasound-method-risk-and-protective-factor-analysis-in-11-820-universally-screened-newborns
#8
Mohammad Schams, Rob Labruyère, Anne Zuse, Mikolaj Walensi
The essential role of ultrasound examinations in diagnosis and treatment of developmental dysplasia of the hip (DDH) is widely accepted while the weighting and correlation of protective factors and perinatal risk for DDH still give rise to debate. Our aim was to investigate the impact of single and twofold combined risk and protective factors on the newborns' hip maturity, assessed with the Graf ultrasound method. Therefore, data sets of 11,820 universally screened newborns were analyzed. Univariate and logistic regression analyses were performed to correlate risk and protective factors with mature or immature but appropriate for age and pathologic hip types...
July 17, 2017: European Journal of Pediatrics
https://www.readbyqxmd.com/read/28694036/-priority-setting-and-rationing-of-pharmaceuticals-an-experimental-analysis-of-discussion-processes
#9
Ines Aumann, Svenja Litzkendorf, Kathrin Damm, J Matthias Graf von der Schulenburg
BACKGROUND/OBJECTIVE: In the face of rising expenditure among statutory sickness funds in Germany it is necessary to start a discussion about priority setting in the healthcare system. For a long time this issue has been avoided in healthcare debates. As a result, normative directives are still missing, which can lead to priority setting among healthcare providers in daily healthcare practice. Prioritization can be conducted at three different levels: at the government (macro), the institutional (meso), and the patient (micro) level...
August 2017: Zeitschrift Für Evidenz, Fortbildung und Qualität Im Gesundheitswesen
https://www.readbyqxmd.com/read/28659574/validation-of-weak-biological-effects-by-round-robin-experiments-cytotoxicity-biocompatibility-of-sio2-and-polymer-nanoparticles-in-hepg2-cells
#10
Lisa Landgraf, Daniel Nordmeyer, Peter Schmiel, Qi Gao, Sandra Ritz, Julia S Gebauer, Stefan Graß, Silvia Diabaté, Lennart Treuel, Christina Graf, Eckart Rühl, Katharina Landfester, Volker Mailänder, Carsten Weiss, Reinhard Zellner, Ingrid Hilger
All over the world, different types of nanomaterials with a diversified spectrum of applications are designed and developed, especially in the field of nanomedicine. The great variety of nanoparticles (NPs), in vitro test systems and cell lines led to a vast amount of publications with conflicting data. To identify the decisive principles of these variabilities, we conducted an intercomparison study of collaborating laboratories within the German DFG Priority Program SPP1313, using well-defined experimental parameters and well-characterized NPs...
June 28, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28616821/re-irradiation-after-gross-total-resection-of-recurrent-glioblastoma-spatial-pattern-of-recurrence-and-a-review-of-the-literature-as-a-basis-for-target-volume-definition
#11
Christoph Straube, Greeshma Elpula, Jens Gempt, Julia Gerhardt, Stefanie Bette, Claus Zimmer, Friederike Schmidt-Graf, Bernhard Meyer, Stephanie E Combs
BACKGROUND: Currently, patients with gross total resection (GTR) of recurrent glioblastoma (rGBM) undergo adjuvant chemotherapy or are followed up until progression. Re-irradiation, as one of the most effective treatments in macroscopic rGBM, is withheld in this situation, as uncertainties about the pattern of re-recurrence, the target volume, and also the efficacy of early re-irradiation after GTR exist. METHODS: Imaging and clinical data from 26 consecutive patients with GTR of rGBM were analyzed...
June 14, 2017: Strahlentherapie und Onkologie: Organ der Deutschen Röntgengesellschaft ... [et Al]
https://www.readbyqxmd.com/read/28609482/quickly-identifying-identical-and-closely-related-subjects-in-large-databases-using-genotype-data
#12
Yumi Jin, Alejandro A Schäffer, Stephen T Sherry, Michael Feolo
Genome-wide association studies (GWAS) usually rely on the assumption that different samples are not from closely related individuals. Detection of duplicates and close relatives becomes more difficult both statistically and computationally when one wants to combine datasets that may have been genotyped on different platforms. The dbGaP repository at the National Center of Biotechnology Information (NCBI) contains datasets from hundreds of studies with over one million samples. There are many duplicates and closely related individuals both within and across studies from different submitters...
2017: PloS One
https://www.readbyqxmd.com/read/28607329/effect-of-long-term-physical-activity-on-pcsk9-high-and-low-density-lipoprotein-cholesterol-and-lipoprotein-a-levels-a-prospective-observational-trialeffect-of-long-term-physical-activity-on-pcsk9-high-and-low-density-lipoprotein-cholesterol-and-l
#13
Michael Sponder, Ioana-Alexandra Campean, Daniel Dalos, Michael Emich, Monika Fritzer-Szekeres, Brigitte Litschauer, Jutta Bergler-Klein, Senta Graf, Jeanette Strametz-Juranek
INTRODUCTION    Since proprotein convertase subtilisin/kexin type 9 (PCSK9) inhibitors were introduced to the market, the interest in PCSK9 metabolism has increased dramatically. OBJECTIVES    We investigated prospectively the influence of long-term physical activity on PCSK9, highand low-density lipoprotein cholesterol (HDL-C and LDL-C, respectively), and lipoprotein(a) levels [Lp(a)]. PATIENTS AND METHODS    A total of 109 participants were recruited and instructed to increase their sport pensum by 75 min/wk of vigorous-intensity or 150 min/wk of moderate-intensity endurance training (or a mixture) within the calculated training pulse for 8 months...
August 9, 2017: Polish Archives of Internal Medicine
https://www.readbyqxmd.com/read/28594169/biosynthesis-of-fluorinated-peptaibols-using-a-site-directed-building-block-incorporation-approach
#14
José Rivera-Chávez, Huzefa A Raja, Tyler N Graf, Joanna E Burdette, Cedric J Pearce, Nicholas H Oberlies
Synthetic biological approaches, such as site-directed biosynthesis, have contributed to the expansion of the chemical space of natural products, making possible the biosynthesis of unnatural metabolites that otherwise would be difficult to access. Such methods may allow the incorporation of fluorine, an atom rarely found in nature, into complex secondary metabolites. Organofluorine compounds and secondary metabolites have both played pivotal roles in the development of drugs; however, their discovery and development are often via nonintersecting tracks...
June 23, 2017: Journal of Natural Products
https://www.readbyqxmd.com/read/28589354/crispr-cas9-mediated-in-vitro-mutagenesis-in-gc-like-b-cells
#15
Van Trung Chu, Robin Graf, Klaus Rajewsky
The CRISPR/Cas9 technology has developed into a powerful tool for genome editing, both in terms of gene silencing and the insertion of precise mutations. However, the application of CRISPR/Cas9-mediated mutagenesis in primary immune cells, in particular in B cells, is still in its infancy because of the difficulty to deliver the CRISPR/Cas9 system into these cells. Here, we describe a new method to use CRISPR/Cas9 for manipulating genes in germinal center (GC)-like B cells in vitro. We isolated Cas9-expressing B cells from R26-Cas9iGFP/+ mice (expressing Cas9 constitutively from the Rosa26 locus) and mixed them with control B cells...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28587671/integrative-taxonomy-by-molecular-species-delimitation-multi-locus-data-corroborate-a-new-species-of-balkan-drusinae-micro-endemics
#16
Simon Vitecek, Mladen Kučinić, Ana Previšić, Ivana Živić, Katarina Stojanović, Lujza Keresztes, Miklós Bálint, Felicitas Hoppeler, Johann Waringer, Wolfram Graf, Steffen U Pauls
BACKGROUND: Taxonomy offers precise species identification and delimitation and thus provides basic information for biological research, e.g. through assessment of species richness. The importance of molecular taxonomy, i.e., the identification and delimitation of taxa based on molecular markers, has increased in the past decade. Recently developed exploratory tools now allow estimating species-level diversity in multi-locus molecular datasets. RESULTS: Here we use molecular species delimitation tools that either quantify differences in intra- and interspecific variability of loci, or divergence times within and between species, or perform coalescent species tree inference to estimate species-level entities in molecular genetic datasets...
June 6, 2017: BMC Evolutionary Biology
https://www.readbyqxmd.com/read/28553000/psychosocial-stress-course-of-pregnancy-and-pregnancy-outcomes-in-the-context-of-the-provision-of-sexual-services
#17
Elisabeth Simoes, Johannes Gostomzyk, Sara Yvonne Brucker, Joachim Graf
Introduction There has been very little medical research into pregnancies which occur in the context of prostitution, even though the associated health risks for mother and child, e.g. violence or maternal drug abuse, are well known. The aim of this study was to compile and summarize what is known (inter-)nationally about this topic and identify key points of support as part of a uniform standard of healthcare in pregnancy. Material and Methods A selective search of the literature was done in Pubmed and Livivo/Medpilot and in the databases NIH, Cochrane, DARE, NHSEED and HTA on the factors influencing preterm delivery...
April 2017: Geburtshilfe und Frauenheilkunde
https://www.readbyqxmd.com/read/28538419/magnetic-resonance-imaging-evaluation-of-the-labrum-to-predict-acetabular-development-in-developmental-dysplasia-of-the-hip-a-strobe-compliant-study
#18
Yasuhiro Shirai, Kenjiro Wakabayashi, Ikuo Wada, Yoshiaki Tsuboi, Myongsu Ha, Takanobu Otsuka
Recently, more attention has been paid to the role of the acetabular labrum. Therefore, we designed a retrospective cohort study of patients with residual hip dysplasia (RHD) who underwent magnetic resonance imaging (MRI). The objective of this study was to investigate an association between the MRI appearance of the labrum before school age and the natural history of RHD.We retrospectively investigated 45 hips of 40 patients who underwent MRI at about 3 and 4 years of age for RHD and were conservatively followed up with until 6 years of age or older...
May 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/28453261/comparison-of-metabolomics-approaches-for-evaluating-the-variability-of-complex-botanical-preparations-green-tea-camellia-sinensis-as-a-case-study
#19
Joshua J Kellogg, Tyler N Graf, Mary F Paine, Jeannine S McCune, Olav M Kvalheim, Nicholas H Oberlies, Nadja B Cech
A challenge that must be addressed when conducting studies with complex natural products is how to evaluate their complexity and variability. Traditional methods of quantifying a single or a small range of metabolites may not capture the full chemical complexity of multiple samples. Different metabolomics approaches were evaluated to discern how they facilitated comparison of the chemical composition of commercial green tea [Camellia sinensis (L.) Kuntze] products, with the goal of capturing the variability of commercially used products and selecting representative products for in vitro or clinical evaluation...
May 26, 2017: Journal of Natural Products
https://www.readbyqxmd.com/read/28451858/biomechanical-in-vitro-comparison-of-radiofrequency-kyphoplasty-and-balloon-kyphoplasty
#20
Gerhard Achatz, Hans-Joachim Riesner, Benedikt Friemert, Raimund Lechner, Nicolas Graf, Hans-Joachim Wilke
PURPOSE: Balloon kyphoplasty (BK) has emerged as a popular method for treating osteoporosis vertebral compression fractures (OVCFs). In response to several shortcomings of BK, alternative methods have been introduced, among which is radiofrequency kyphoplasty (RFK). Biomechanical comparisons of BK and RFK are very sparse. The purpose of this study was to perform a biomechanical study in which BK and RFK are compared. METHODS: Each of the two study groups comprised six specimens prepared from two functional spinal units (FSUs) cut from fresh-frozen cadaveric spines (3 of T9-T11 and 3 of T12-L2)...
April 27, 2017: European Spine Journal
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