keyword
MENU ▼
Read by QxMD icon Read
search

Rock1

keyword
https://www.readbyqxmd.com/read/29791873/rhoa-rock-signaling-regulates-smooth-muscle-phenotypic-modulation-and-vascular-remodeling-via-the-jnk-pathway-and-vimentin-cytoskeleton
#1
Lian Tang, Fan Dai, Yan Liu, Xiaoqiang Yu, Chao Huang, Yuqin Wang, Wenjuan Yao
The RhoA/ROCK signaling pathway regulates cell morphology, adhesion, proliferation, and migration. In this study, we investigated the regulatory role of RhoA/ROCK signaling on PDGF-BB-mediated smooth muscle phenotypic modulation and vascular remodeling and clarified the molecular mechanisms behind these effects. PDGF-BB treatment induced the activation of RhoA, ROCK, PDGF-Rβ, and the expression of PDGF-Rβ in HA-VSMCs (human aortic vascular smooth muscle cells). PDGF-Rβ inhibition and RhoA suppression blocked PDGF-BB-induced RhoA activation and ROCK induction...
May 20, 2018: Pharmacological Research: the Official Journal of the Italian Pharmacological Society
https://www.readbyqxmd.com/read/29790629/long-non-coding-rna-neat1-promoted-ovarian-cancer-cells-metastasis-via-regulating-of-mir-382-3p-rock1-axial
#2
Yangcheng Liu, Yong Wang, Xinming Fu, Zhi Lu
Long non-coding RNAs (lncRNAs) are extensively involved in various malignant tumors including ovarian cancer (OC). In the present study, we focused on the expression and function of nuclear enriched abundant transcript 1 (NEAT1) work on ovarian cancer cells' metastasis. We demonstrated that NEAT1 was up-regulated in OC tissue specimens and cell lines. Also, we revealed that depression of NEAT1 inhibited OC cells' metastasis and the expression of Rho associated coiled-coil containing protein kinase 1 (ROCK1) which was a metastasis-related gene...
May 23, 2018: Cancer Science
https://www.readbyqxmd.com/read/29789524/hirsutine-induces-mptp-dependent-apoptosis-through-rock1-pten-pi3k-gsk3%C3%AE-pathway-in-human-lung-cancer-cells
#3
Rong Zhang, Guobing Li, Qian Zhang, Qin Tang, Jingbin Huang, Changpeng Hu, Yali Liu, Qing Wang, Wuyi Liu, Ning Gao, Shiwen Zhou
Hirsutine extracted from Uncaria rhynchophylla has been shown to exhibit anti-cancer activity. However, the molecular mechanism by which hirsutine exhibits anti-lung cancer activity remains unclear. In the present study, we showed that hirsutine induces apoptosis in human lung cancer cells via loss of mitochondrial membrane potential (∆ψm), adenosine triphosphate (ATP) depletion, ROS production, as well as cytochrome c release. Dephosphorylation of GSK3β is involved in hirsutine-mediated mitochondrial permeability transition pore (mPTP) opening through ANT1/CypD interaction...
May 22, 2018: Cell Death & Disease
https://www.readbyqxmd.com/read/29786744/mir%C3%A2-133b-regulates-proliferation-and-apoptosis-in-high%C3%A2-glucose%C3%A2-induced-human-retinal-endothelial-cells-by-targeting-ras-homolog-family-member-a
#4
Jun Yao, Jihong Wang, Yong Yao, Kelei Wang, Qianqian Zhou, Ying Tang
The aim of the present study was to investigate the role of microRNA (miR)‑133b in high‑glucose‑induced human retinal endothelial cells (hRECs), particularly regarding its potential targeting of ras homolog family member A (RhoA). To establish the high‑glucose‑induced diabetic retinopathy (DR) model, hRECs were cultured in high‑glucose medium for 1, 2 and 3 days. An Annexin allophycocyanin (APC)/7‑aminoactinomycin D (7‑AAD) staining assay was performed to measure the apoptosis of hRECs. Next, the cells were transfected with miR‑133b inhibitors or mimics, and the cell proliferation and apoptosis were measured by MTT and Annexin‑APC/7‑AAD staining assays, respectively...
May 18, 2018: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/29785050/the-tumor-suppressor-dlc1-inhibits-cancer-progression-and-oncogenic-autophagy-in-hepatocellular-carcinoma
#5
Hui-Ta Wu, Cheng-Rong Xie, Jie Lv, He-Qiang Qi, Fei Wang, Sheng Zhang, Qin-Liang Fang, Fu-Qiang Wang, Yu-Yan Lu, Zhen-Yu Yin
Downregulation of deleted in liver cancer 1 (DLC1) is associated with poor prognosis of various cancers, but its functional mechanisms in hepatocellular carcinoma (HCC) remains unclear. In the present study, we investigated the roles of DLC1 in tumor progression and autophagy of HCC. We found that DLC1 was frequently downregulated in HCC tissues. Underexpression of DLC1 correlated with AFP level, vascular invasion, poor differentiation, and poor prognosis. In vitro assays revealed that DLC1 not only suppressed the proliferation, migration, and invasion of HCC cells, but also inhibited autophagy of HCC cells...
May 21, 2018: Laboratory Investigation; a Journal of Technical Methods and Pathology
https://www.readbyqxmd.com/read/29768267/type-2-diabetes-promotes-cell-centrosome-amplification-via-akt-ros-dependent-signalling-of-rock1-and-14-3-3%C3%AF
#6
Pu Wang, Yu Cheng Lu, Jie Wang, Lan Wang, Hanry Yu, Yuan Fei Li, Alice Kong, Juliana Chan, Shaochin Lee
BACKGROUND/AIMS: Type 2 diabetes is associated with oxidative stress and DNA damage which can cause centrosome amplification. Thus, the study investigated centrosome amplification in type 2 diabetes and the underlying mechanisms. METHODS: Centrosome numbers in human peripheral blood mononuclear blood cells (PBMC) from healthy subjects and patients with type 2 diabetes were compared to access the association between type 2 diabetes and centrosome amplification. Colon cancer cells were used to investigate the molecular mechanisms underlying the centrosome amplification triggered by high glucose, insulin and palmitic acid...
May 11, 2018: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29765452/effects-and-potential-mechanism-of-atorvastatin-treatment-on-lp-pla2-in-rats-with-dyslipidemia
#7
Dongdan Zheng, Anping Cai, Rulin Xu, Zhuocheng Mai, Yingling Zhou, Fanfang Zeng, Liwen Li, Weiyi Mai
Introduction: The effects of statins on lipoprotein-associated phospholipase A2 (Lp-PLA2) are controversial, and the present study aimed to investigate whether atorvastatin could reduce Lp-PLA2 in rats with dyslipidemia. Material and methods: A high-fat and high-cholesterol diet was prescribed to produce a dyslipidemia model. Thereafter, low-dose atorvastatin (5 mg/kg/day), high-dose atorvastatin (20 mg/kg/day) or saline (without-treatment group) was prescribed for 14 days...
April 2018: Archives of Medical Science: AMS
https://www.readbyqxmd.com/read/29753317/long-noncoding-rna-dancr-working-as-a-competitive-endogenous-rna-promotes-rock1-mediated-proliferation-and-metastasis-via-decoying-of-mir-335-5p-and-mir-1972-in-osteosarcoma
#8
Yong Wang, Xiandong Zeng, Ningning Wang, Wei Zhao, Xi Zhang, Songling Teng, Yueyan Zhang, Zhi Lu
BACKGROUND: Accumulating evidences indicate that non-coding RNAs (ncRNAs) including long non-coding RNAs (lncRNAs) and microRNAs (miRNAs) acting as crucial regulators in osteosarcoma (OS). Previously, we reported that Rho associated coiled-coil containing protein kinase 1 (ROCK1), a metastatic-related gene was negatively regulated by microRNA-335-5p (miR-335-5p) and work as an oncogene in osteosarcoma. Whether any long non-coding RNAs participate in the upstream of miR-335-5p/ROCK1 axial remains unclear...
May 12, 2018: Molecular Cancer
https://www.readbyqxmd.com/read/29751634/rho-a-regulates-epidermal-growth-factor-induced-human-osteosarcoma-mg63-cell-migration
#9
Jinyang Wang, Lei Zhang, Rongmei Qu, Lin Zhang, Wenhua Huang
Osteosarcoma, the most common primary bone tumor, occurs most frequently in children and adolescents and has a 5-year survival rate, which is unsatisfactory. As epidermal growth factor receptor (EGFR) positively correlates with TNM (tumor-node-metastasis) stage in osteosarcoma, EGFR may play an important role in its progression. The purpose of this study was to explore potential mechanisms underlying this correlation. We found that EGF promotes MG63 cell migration and invasion as well as stress fiber formation via Rho A activation and that these effects can be reversed by inhibiting Rho A expression...
May 11, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29749520/silencing-of-urg11-expression-inhibits-the-proliferation-and-epithelial%C3%A2-mesenchymal-transition-in-benign-prostatic-hyperplasia-cells-via-the-rhoa-rock1-pathway
#10
Guanying Zhang, Feng Zhu, Guangye Han, Zeyu Li, Quanfeng Yu, Zhenhui Li, Jianchang Li
Upregulated gene 11 (URG11) represents a gene upregulated by hepatitis B virus X protein and is involved in the biological processes of multifarious tumors. The present study aimed to investigate the protective effects and regulatory mechanisms of URG11 in benign prostatic hyperplasia (BPH). URG11, Ras homolog family member A (RhoA) and Rho‑associated protein kinase 1 (ROCK1) expression was detected in patients with BPH using reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR). Furthermore, URG11 expression was silenced using URG11‑targeting small interfering RNAs...
May 8, 2018: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29725411/efficacy-of-extracts-of-celastrus-orbiculatus-in-suppressing-migration-and-invasion-by-inhibiting-the-ezh2-rock1-signaling-pathway-in-human-nasopharyngeal-carcinoma
#11
Xuanyi Wang, Yuxiang Huang, Yong Chen, Yong Ma, Fei Yang, Yayun Qian, Xiaojun Dai, Lede Tao, Haibo Wang, Renhua Guo, Yanqing Liu
Celastrus orbiculatus extract (COE) has been used in folk medicine in China for the treatment of a number of diseases. In the laboratory, COE exhibits a variety of anticancer functions, including inhibition of metastasis. However, the underlying molecular anti-metastatic mechanism in nasopharyngeal carcinoma (NPC) cells remains unclear. The aim of the present study was to determine whether the anti-metastatic effect of COE was involved in inhibiting migration and invasion of human NPC cells. In vitro , cell viability and apoptosis of 5-8F cells were analyzed using an MTS assay and flow cytometry, respectively...
May 2018: Oncology Letters
https://www.readbyqxmd.com/read/29724193/chronophin-regulates-active-vitamin-b6-levels-and-transcriptomic-features-of-glioblastoma-cell-lines-cultured-under-non-adherent-serum-free-conditions
#12
Markus Schulze, Maria Hutterer, Anja Sabo, Sabine Hoja, Julia Lorenz, Tanja Rothhammer-Hampl, Christel Herold-Mende, Lucia Floßbach, Camelia Monoranu, Markus J Riemenschneider
BACKGROUND: The phosphatase chronophin (CIN/PDXP) has been shown to be an important regulator of glioma cell migration and invasion. It has two known substrates: p-Ser3-cofilin, the phosphorylated form of the actin binding protein cofilin, and pyridoxal 5'-phosphate, the active form of vitamin B6. Phosphoregulation of cofilin, among other functions, plays an important role in cell migration, whereas active vitamin B6 is a cofactor for more than one hundred enzymatic reactions. The role of CIN has yet only been examined in glioblastoma cell line models derived under serum culture conditions...
May 3, 2018: BMC Cancer
https://www.readbyqxmd.com/read/29716922/meis1-and-meis2-expression-and-prostate-cancer-progression-a-role-for-hoxb13-binding-partners-in-metastatic-disease
#13
Raj R Bhanvadia, Calvin VanOpstall, Hannah Brechka, Nimrod S Barashi, Marc Gillard, Erin M McAuley, Juan Apiz, Gladell P Paner, Wen-Ching Chan, Jorge Andrade, Angelo Demarzo, Misop Han, Russell Z Szmulewitz, Donald J Vander Griend
PURPOSE: Germline mutations within the MEIS-interaction domain of HOXB13 have implicated a critical function for MEIS-HOX interactions in prostate cancer etiology and progression.  The functional and predictive role of changes in MEIS expression within prostate tumor progression, however, remain largely unexplored. EXPERIMENTAL DESIGN: Here we utilize RNA expression datasets, annotated tissue microarrays, and cell-based functional assays to investigate the role of MEIS1 and MEIS2 in prostate cancer and metastatic progression...
May 1, 2018: Clinical Cancer Research: An Official Journal of the American Association for Cancer Research
https://www.readbyqxmd.com/read/29710554/microrna-361-5p-inhibits-papillary-thyroid-carcinoma-progression-by-targeting-rock1
#14
Rui Li, Bingfei Dong, Zhengmin Wang, Tao Jiang, Guang Chen
MicroRNAs function as key regulators in various human cancers, including papillary thyroid cancer (PTC). MiR-361-5p has been proved to be a tumor suppressor in multiple cancers. However, the function of miR-361-5p in PTC remains unknown. In this study, we aimed to determine the function of miR-361-5p in PTC progression, and elaborate the mechanism by which miR-361-5p acts in PTC. Here, we report that miR-361-5p expression levels were significantly downregulated in PTC tissues and cell lines, as detected by reverse transcription-quantitative polymerase chain reaction (qRT-PCR) analysis...
April 3, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29702091/inhibition-of-malat1-sensitizes-liver-cancer-cells-to-5-flurouracil-by-regulating-apoptosis-through-ikk%C3%AE-nf-%C3%AE%C2%BAb-pathway
#15
Zhenguo Pan, De-Gang Ji, Lian-Yue Guan, Xiao Luo, Feng Ma, Bin Yang, Hong-Yu Liu
Metastasis associated lung adenocarcinoma transcript 1 (MALAT1) is involved in tumor cell growth process. However, its role and molecular mechanism in liver cancer is still not fully understood. In this study, we found that MALAT1 was significantly expressed in liver cancer cell lines. And knockdown of MALAT1 suppressed proliferation, migration and invasion of HepG2 cells, accompanied with decrease of Rho-associated coiled-coil-forming protein kinase 1 (ROCK1), α-smooth muscle actin (α-SMA), N-cadherin, Vimentin and TWIST...
April 24, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29669760/rho-kinase-inhibition-by-at13148-blocks-pancreatic-ductal-adenocarinoma-invasion-and-tumor-growth
#16
Nicola Rath, June Munro, Marie Fa Cutiongco, Alicja Jagiełło, Nikolaj Gadegaard, Lynn McGarry, Mathieu Unbekandt, Evdokia Michalopoulou, Jurre J Kamphorst, David Sumpton, Gillian Mackay, Claire Vennin, Marina Pajic, Paul Timpson, Michael F Olson
The high mortality of pancreatic cancer demands that new therapeutic avenues be developed. The orally available small molecule inhibitor AT13148 potently inhibits ROCK1 and ROCK2 kinases that regulate the actomyosin cytoskeleton. We previously reported that ROCK kinase expression increases with human and mouse pancreatic cancer progression and that conditional ROCK activation accelerates mortality in a genetically modified LSL-KrasG12D; LSL-p53R172H; Pdx1-Cre; (KPC) mouse pancreatic cancer model. In this study, we show that treatment of KPC mouse and human TKCC5 patient-derived pancreatic tumor cells with AT13148, as well as the ROCK selective inhibitors Y27632 and H1152, act comparably in blocking ROCK substrate phosphorylation...
April 18, 2018: Cancer Research
https://www.readbyqxmd.com/read/29669735/ctgf-vegfa-activated-fibroblasts-promote-tumor-migration-through-micro-environmental-modulation
#17
Wei Wu, Esther A Zaal, Celia R Berkers, Simone Lemeer, Albert J R Heck
Fibroblast activation is associated with tumour progression and implicated in metastasis, but the initial triggering signals required to kick-start this process remain largely unknown. Since small cancerous lesions share limited physical contact with neighboring fibroblasts, we reasoned the first tumour-derived signal for fibroblast activation should be secreted and diffusible. By pulsed metabolic labeling and click-chemistry based affinity enrichment, we sieved through the ductal carcinoma secretome for potential fibroblast activators...
April 18, 2018: Molecular & Cellular Proteomics: MCP
https://www.readbyqxmd.com/read/29669280/the-long-non-coding-rna-lnc-31-interacts-with-rock1-mrna-and-mediates-its-yb-1-dependent-translation
#18
Dacia Dimartino, Alessio Colantoni, Monica Ballarino, Julie Martone, Davide Mariani, Johannes Danner, Astrid Bruckmann, Gunter Meister, Mariangela Morlando, Irene Bozzoni
Cytoplasmic long non-coding RNAs have been shown to act at many different levels to control post-transcriptional gene expression, although their role in translational control is poorly understood. Here, we show that lnc-31, a non-coding RNA required for myoblast proliferation, promotes ROCK1 protein synthesis by stabilizing its translational activator, YB-1. We find that lnc-31 binds to the Rock1 mRNA as well as to the YB-1 protein and that translational activation requires physical interaction between the two RNA species...
April 17, 2018: Cell Reports
https://www.readbyqxmd.com/read/29649319/unraveling-endothelin-1-induced-hypercontractility-of-human-pulmonary-artery-smooth-muscle-cells-from-patients-with-pulmonary-arterial-hypertension
#19
Jamie L Wilson, Rod Warburton, Linda Taylor, Deniz Toksoz, Nicholas Hill, Peter Polgar
Contraction of human pulmonary artery smooth muscle cells (HPASMC) isolated from pulmonary arterial hypertensive (PAH) and normal (non-PAH) subject lungs was determined and measured with real-time electrical impedance. Treatment of HPASMC with vasoactive peptides, endothelin-1 (ET-1) and bradykinin (BK) but not angiotensin II, induced a temporal decrease in the electrical impedance profile mirroring constrictive morphological change of the cells which typically was more robust in PAH as opposed to non-PAH cells...
2018: PloS One
https://www.readbyqxmd.com/read/29642915/adipokine-rbp4-drives-ovarian-cancer-cell-migration
#20
Yanyan Wang, Yilin Wang, Zhenyu Zhang
BACKGROUND: Obesity has been linked to several types of cancers including ovarian cancer. Retinol binding protein 4 (RBP4) is an adipokine that drives the development of hyperinsulinemia and type II diabetes in obesity patients and animals. Previously, we have identified RBP4 as a serum marker for ovarian cancer. Here we further explored the consequence of RBP4 upregulation in ovarian cancer cells and its molecular mechanism. RESULTS: Our results show that RBP4 is overexpressed in ovarian cancer cells to the same extent as in adipose tissues...
April 11, 2018: Journal of Ovarian Research
keyword
keyword
85432
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"