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https://www.readbyqxmd.com/read/29777815/enhanced-antibacterial-activity-mechanical-and-physical-properties-of-alginate-hydroxyapatite-bionanocomposite-film
#1
Bahador Safikhani Gholizadeh, Foad Buazar, Seyed Mehdi Hosseini, Seyed Mohammad Mousavi
The effect of hydroxyapatite nanoparticles (HA NPs) contents on the antibacterial activity, physical, and mechanical properties of sodium alginate (SA) film was scrutinized. The samples were prepared via incorporation of three different concentrations of HA NPs (1%, 3%, and 5%) into alginate solution at ambient temperature. In all SA/HA matrices, HA NPs demonstrated considerable bactericidal activity against foodborne pathogen during 12 days. The highest antibacterial effect of SA film was obtained in the presence of 5%HA NPs content and showed 3 CFU/mg reduction...
May 16, 2018: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29765999/genetic-variability-of-antigen-b8-1-among-echinococcus-granulosus-isolates-from-human-cattle-and-sheep-in-fars-province-southern-iran
#2
Asieh Fatemi Esfedan, Bahador Sarkari, Fataneh Mikaeili
Background: Cystic echinococcosis (CE), known as hydatid cyst, is a zoonotic parasitic infection caused by the larval stage of Echinococcus granulosus (E. granulosus) . Antigen B, the major component of hydatid cyst fluid, is encoded by members of a multigene family. The present study aimed to evaluate the genetic diversity of the gene encoding antigen B8/1 ( EgAgB8/1 ) among the main intermediate hosts of E. granulosus . Methods: Twenty-eight hydatid cyst isolates (10 sheep, 9 human, and 9 cattle) were collected in Fars province, Iran...
April 2018: Reports of Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/29743011/procaine-and-saline-have-similar-effects-on-articular-cartilage-and-synovium-in-rat-knee
#3
Aysun Ankay Yilbas, Basak Akca, Berkem Buyukakkus, Elham Bahador Zirh, Dilara Zeybek, Filiz Uzumcugil, Fatma Saricaoglu
BACKGROUND: Intra-articular local anaesthetics are widely used for providing postoperative analgesia and decreasing the need for opioids. Procaine has proven positive effects in carpal tunnel syndrome and chondromalacia patella. However, the effect of procaine on articular cartilage has not yet been studied. The aim of this study was to evaluate the effects of intra-articular procaine injection on the articular cartilage and the synovium. METHODS: Twenty adult Sprague-Dawley rats were enrolled in the study...
May 9, 2018: BMC Anesthesiology
https://www.readbyqxmd.com/read/29709604/ex-vivo-assessment-of-synergic-effect-of-chlorhexidine-for-enhancing-antimicrobial-photodynamic-therapy-efficiency-on-expression-patterns-of-biofilm-associated-genes-of-enterococcus-faecalis
#4
Behnam Bolhari, Maryam Pourhajibagher, Farzaneh Bazarjani, Nasim Chiniforush, Mehdi Rostami Rad, Salma Pirmoazen, Abbas Bahador
BACKGROUND: It has clearly demonstrated that Enterococcus faecalis, as a persistent microorganism is the major agent in the etiopatogeny of endodontic infections. Recently, the limitations of conventional endodontic therapy have given rise to many attempts to introduce antimicrobial photodynamic therapy (aPDT) as an alternative treatment. The aim of this study was to analyze the ex vivo effect of aPDT in combination with 2.0 % chlorhexidine (CHX) as a conventional therapy on colony count and expression patterns of genes associated with biofilm formation of E...
April 27, 2018: Photodiagnosis and Photodynamic Therapy
https://www.readbyqxmd.com/read/29687151/anti-cancer-parasporin-toxins-of-new-bacillus-thuringiensis-against-human-colon-hct-116-and-blood-ccrf-cem-cancer-cell-lines
#5
Elham Moazamian, Nima Bahador, Negar Azarpira, Manoochehr Rasouli
Bacillus thuringiensis is one of the most important microorganisms used against cancer cell lines in latest studies all over the world. This study aims to perform the isolation, molecular identification, and to identify novel B. thuringiensis strains that specifically targeting human cancer cell-killing activities in Iran. A total of 88 B. thuringiensis isolates were recovered from Iran. Upon the treatment of the non-hemolytic crystal proteins by proteinase K, five isolates belonging to three biotypes, thuringiensis, kurstaki and sotto of B...
April 23, 2018: Current Microbiology
https://www.readbyqxmd.com/read/29676701/single-center-long-term-results-of-pediatric-liver-transplantation
#6
Mohammad Hussein Al Sayyed, Alireza Shamsaeefar, Saman Nikeghbalian, Seyyed Mohsen Dehghani, Ali Bahador, Masood Dehghani, Razieh Rasekh, Siavash Gholami, Bahareh Khosravi, Seyyed Ali Malek Hosseini
OBJECTIVES: Liver replacement continues to be the only definitive mode of therapy for children with end-stage liver disease. However, it remains challenging because of the rare donor organs, complex surgical demands, and the necessity to prevent long-term complications. Our objectives were to analyze 16 years of experience in the Shiraz University Organ Transplant Center. MATERIALS AND METHODS: We retrospectively analyzed the records of 752 patients (< 18 years old) who underwent orthotopic liver transplant at our center over a 16-year period...
April 20, 2018: Experimental and Clinical Transplantation
https://www.readbyqxmd.com/read/29657409/dna-extraction-from-hydatid-cyst-protoscolices-comparison-of-five-different-methods
#7
Afshin Barazesh, Bahador Sarkari, Sepideh Ebrahimi, Mehdi Hami
Aim: The current study aimed to find out a simple, practical and high throughput DNA isolation method for extraction of DNA from hydatid cyst samples. Materials and Methods: Cattle and sheep isolate of hydatid cysts were obtained from the slaughterhouse, and hydatid fluid and protoscolices were collected in a sterile condition. Protoscolices were washed, 3 times with phosphate buffered saline, and DNA was extracted by different methods including manual extraction with freeze/thawing and phenol-chloroform, Triton X-100 extraction, and by a commercial kit (YTA, Yekta Tajhiz Azma, Iran) with three different modifications in the kit's manufacturer instructions...
February 2018: Veterinary World
https://www.readbyqxmd.com/read/29653462/docosahexaenoic-acid-reversed-atherosclerotic-changes-in-human-endothelial-cells-induced-by-palmitic-acid-in-vitro
#8
Saeede Karbasforush, Alireza Nourazarian, Masoud Darabi, Reza Rahbarghazi, Fatemeh Khaki-Khatibi, Çıgır Biray Avci, Leila Salimi, Bakiye Goker Bagca, Tanaz Novin Bahador, Aysa Rezabakhsh, Majid Khaksar
Abnormal activity of atherosclerotic endothelial cells paving luminal surface of blood vessels has been described in many diseases. It has been reported that natural polyunsaturated fatty acids such as docosahexaenoic acid exert therapeutic effects in atherosclerotic condition. Human umbilical vein endothelial cells were treated with 1mM palmitic acid for 48 hours and exposed to 40μM docosahexaenoic acid for the next 24 hours. Real-time polymerase chain reaction analysis was used to measure the expression of PTX3, iNOS, and eNOS...
April 13, 2018: Cell Biochemistry and Function
https://www.readbyqxmd.com/read/29627953/correction-to-solidification-of-hydatid-cyst-fluid-with-an-injectable-chitosan-carboxymethylcellulose-%C3%AE-glycerophosphate-hydrogel-for-effective-control-of-spillage-during-aspiration-of-hydatid-cysts
#9
Mostafa D A Azadi, Shadi Hassanajili, Khalil Zarrabi, Bahador Sarkari
The original version of this article unfortunately contained a mistake: the spelling of the Shadi Hassanajili's name was incorrect. The corrected name is given above.
April 7, 2018: Progress in Biomaterials
https://www.readbyqxmd.com/read/29621968/effects-of-micro-rnas-and-their-targets-in-periodontal-diseases
#10
Samane Bourbour, Maryam Beheshti, Hossein Kazemian, Abbas Bahador
: RNA interference is a strong mechanism of gene silencing which underlies many fields of eukaryotic biology (1). MicroRNAs (miRNAs) are small noncoding RNAs and a gene regulation expression system showing a tissue-specific expression pattern, and inhibits protein synthesis (2). For the first time, miRNA has been seen in Caenorhabditis elegans (3). They play an important role in signaling pathways of various diseases especially periodontal disease and cancers (4). One of the most important diseases in association with gingival infectious is periodontal diseases, which if untreated leads to tooth loss, hence in present paper focus on the effects of MicroRNAs and their targets in disease which was mentioned above...
April 5, 2018: Infectious Disorders Drug Targets
https://www.readbyqxmd.com/read/29621325/stochastic-satisficing-account-of-confidence-in-uncertain-value-based-decisions
#11
Uri Hertz, Bahador Bahrami, Mehdi Keramati
Every day we make choices under uncertainty; choosing what route to work or which queue in a supermarket to take, for example. It is unclear how outcome variance, e.g. uncertainty about waiting time in a queue, affects decisions and confidence when outcome is stochastic and continuous. How does one evaluate and choose between an option with unreliable but high expected reward, and an option with more certain but lower expected reward? Here we used an experimental design where two choices' payoffs took continuous values, to examine the effect of outcome variance on decision and confidence...
2018: PloS One
https://www.readbyqxmd.com/read/29578101/diagnostic-accuracy-of-multiplex-real-time-pcr-approaches-in-compared-with-cultivation-based-detection-methods-monitoring-the-endopathogenic-microbiota-pre-and-post-photo-activated-disinfection
#12
Maryam Pourhajibagher, Abbas Bahador
BACKGROUND: Several microbial species that have been implicated in the pathogenesis of endodontic diseases colonize the infected root canal system. Since the complete removal of endopathogenic agents is essential in endodontic infections therapy, photo-activated disinfection (PAD) is suggested as an alternative method to traditional antimicrobial therapy. Recent studies reported that the molecular methods with low sensitivity and high efficiency to identify fastidious anaerobic endopathogenic microbiota can be replaced by the cultivation-based approaches...
March 22, 2018: Photodiagnosis and Photodynamic Therapy
https://www.readbyqxmd.com/read/29520759/empirical-metronidazole-for-patients-with-severe-bacterial-infection-protocol-for-a-systematic-review
#13
M W Petersen, A Perner, M Bahador, F Sjövall, M H Møller
INTRODUCTION: Anaerobic bacteria are believed to be common pathogens in severe infections. Yet, they are difficult to culture and consequently often unrecognised in clinical infections. Metronidazole is often used empirically for potential anaerobic infections, as the resistance to metronidazole is low. However, disadvantages of metronidazole use exist, including drug interactions, side effects and economical expenses. Currently, the balance between the benefits and harms of empirical metronidazole for severe bacterial infections is unknown...
May 2018: Acta Anaesthesiologica Scandinavica
https://www.readbyqxmd.com/read/29486354/an-in-vivo-evaluation-of-microbial-diversity-before-and-after-the-photo-activated-disinfection-in-primary-endodontic-infections-traditional-phenotypic-and-molecular-approaches
#14
Maryam Pourhajibagher, Abbas Bahador
BACKGROUND: It is essential to identify the root canal microbial diversity and count due to the polymicrobial nature of the primary endodontic infection that is associated with the microbial diversity and increased resistance to the antimicrobial agents. Photo-activated disinfection (PAD), also known as antimicrobial photodynamic therapy, is a new promising non-antibiotic approach, studied to prevent microbial resistance and treatment failure. MATERIALS AND METHODS: In this study, we investigated the effect of PAD on reduction of microbial diversity and count, related with primary endodontic infections...
February 24, 2018: Photodiagnosis and Photodynamic Therapy
https://www.readbyqxmd.com/read/29483899/growth-rate-and-biofilm-formation-ability-of-clinical-and-laboratory-evolved-colistin-resistant-strains-of-acinetobacter-baumannii
#15
Zahra Farshadzadeh, Behrouz Taheri, Sara Rahimi, Saeed Shoja, Maryam Pourhajibagher, Mohammad A Haghighi, Abbas Bahador
Two different mechanisms of resistance to colistin in Acinetobacter baumannii have been described. The first involves the total loss of lipopolysaccharide (LPS) due to mutations in the lpxACD operon, which is involved in the lipid A biosynthesis pathway. The second entails the addition of ethanolamine to the lipid A of the LPS resulting from mutations in the PmrAB two-component system. To evaluate the impact of colistin resistance-associated mutations on antimicrobial resistance and virulence properties, four pairs of clinical and laboratory-evolved colistin-susceptible/colistin-resistant (ColS /ColR ) A...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29482032/carnosine-graphene-oxide-conjugates-decorated-with-hydroxyapatite-as-promising-nanocarrier-for-icg-loading-with-enhanced-antibacterial-effects-in-photodynamic-therapy-against-streptococcus-mutans
#16
Elham Gholibegloo, Ashkan Karbasi, Maryam Pourhajibagher, Nasim Chiniforush, Ali Ramazani, Tayebeh Akbari, Abbas Bahador, Mehdi Khoobi
Antimicrobial photodynamic therapy (aPDT) has been emerged as a noninvasive strategy to remove bacterial contaminants such as S. mutans from the tooth surface. Photosensitizer (PS), like indocyanine green (ICG), plays a key role in this technique which mainly suffers from the poor stability and concentration-dependent aggregation. An appropriate nanocarrier (NC) with enhanced antibacterial effects could overcome these limitations and improve the efficiency of ICG as a PS. In this study, various ICG-loaded NCs including graphene oxide (GO), GO-carnosine (Car) and GO-Car/Hydroxyapatite (HAp) were synthesized and characterized by Fourier Transform Infrared Spectroscopy (FT-IR), X-ray Diffraction (XRD), Filed Emission Scanning Electron Microscopy (FE-SEM), Energy Dispersive Spectroscopy (EDS), Zeta Potential and Ultraviolet-Visible spectrometry (UV-Vis)...
April 2018: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/29473691/assessing-cognitive-dysfunction-in-parkinson-s-disease-an-online-tool-to-detect-visuo-perceptual-deficits
#17
Rimona S Weil, Dietrich S Schwarzkopf, Bahador Bahrami, Stephen M Fleming, Ben M Jackson, Tristam J C Goch, Ayse P Saygin, Luke E Miller, Katerina Pappa, Ivanna Pavisic, Rachel N Schade, Alastair J Noyce, Sebastian J Crutch, Aidan G O'Keeffe, Anette E Schrag, Huw R Morris
BACKGROUND: People with Parkinson's disease (PD) who develop visuo-perceptual deficits are at higher risk of dementia, but we lack tests that detect subtle visuo-perceptual deficits and can be performed by untrained personnel. Hallucinations are associated with cognitive impairment and typically involve perception of complex objects. Changes in object perception may therefore be a sensitive marker of visuo-perceptual deficits in PD. OBJECTIVE: We developed an online platform to test visuo-perceptual function...
April 2018: Movement Disorders: Official Journal of the Movement Disorder Society
https://www.readbyqxmd.com/read/29460180/solidification-of-hydatid-cyst-fluid-with-an-injectable-chitosan-carboxymethylcellulose-%C3%AE-glycerophosphate-hydrogel-for-effective-control-of-spillage-during-aspiration-of-hydatid-cysts
#18
Mostafa D A Azadi, Shadi Hassanjili, Khalil Zarrabi, Bahador Sarkari
Cystic echinococcosis (CE)/hydatid cyst is one of the most important helminthic diseases in the world. The treatment of hydatid cyst ranges from surgical intervention to chemotherapy, although the efficacy of chemotherapy is still unclear. Postoperative complication which results from the spillage of cysts during surgical operation is one of the most important concerns in surgical treatment of hydatid cyst. The aim of the current study was to solidify the hydatid cyst fluid (HCF) with an injectable and thermosensitive chitosan (CS)/carboxymethyl cellulose (CMC)/β-glycerol phosphate (BGP) hydrogel for effective control of spillage during the aspiration of hydatid cysts...
March 2018: Progress in Biomaterials
https://www.readbyqxmd.com/read/29446261/enhanced-linear-array-photoacoustic-beamforming-using-modified-coherence-factor
#19
Moein Mozaffarzadeh, Yan Yan, Mohammad Mehrmohammadi, Bahador Makkiabadi
Photoacoustic imaging (PAI) is a promising medical imaging modality providing the spatial resolution of ultrasound imaging and the contrast of optical imaging. For linear-array PAI, a beamformer can be used as the reconstruction algorithm. Delay-and-sum (DAS) is the most prevalent beamforming algorithm in PAI. However, using DAS beamformer leads to low-resolution images as well as high sidelobes due to nondesired contribution of off-axis signals. Coherence factor (CF) is a weighting method in which each pixel of the reconstructed image is weighted, based on the spatial spectrum of the aperture, to mainly improve the contrast...
February 2018: Journal of Biomedical Optics
https://www.readbyqxmd.com/read/29442884/hydrothermal-growth-of-zno-nanowires-on-uv-nanoimprinted-polymer-structures
#20
Sooyeon Park, Sean A Moore, Jaejong Lee, In-Hyouk Song, Bahador Farshchian, Namwon Kim
Integration of zinc oxide (ZnO) nanowires on miniaturized polymer structures can broaden its application in multi-functional polymer devices by taking advantages of unique physical properties of ZnO nanowires and recent development of polymer microstructures in analytical systems. In this paper, we demonstrate the hydrothermal growth of ZnO nanowires on polymer microstructures fabricated by UV nanoimprinting lithography (NIL) using a polyurethane acrylate (PUA). Since PUA is a siloxane-urethane-acrylate compound containing the alpha-hydroxyl ketone, UV-cured PUA include carboxyl groups, which inhibit and suppress the nucleation and growth of ZnO nanowires on polymer structures...
May 1, 2018: Journal of Nanoscience and Nanotechnology
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