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https://www.readbyqxmd.com/read/28919997/increased-infiltration-and-tolerised-antigen-specific-cd8-tem-cells-in-tumor-but-not-peripheral-blood-have-no-impact-on-survival-of-hcmv-glioblastoma-patients
#1
M Bahador, A Gras Navarro, M A Rahman, M Dominguez-Valentin, S Sarowar, E Ulvestad, G Njølstad, S A Lie, E K Kristoffersen, E Bratland, M Chekenya
Human cytomegalovirus (HCMV) antigens in glioblastoma (GBM) present opportunities for personalised immunotherapy. However, their presence in GBM tissue is still under debate, and evidence of their impact on functional immune responses and prognosis is sparse. Here, we investigated the presence of pp65 (UL83) and immediate early 1 (IE-1) HCMV antigens in a cohort of Norwegian GBM patients (n = 177), using qPCR, immunohistochemistry, and serology. HCMV status was then used to investigate whether viral antigens influenced immune cell phenotype, infiltration, activation and patient survival...
2017: Oncoimmunology
https://www.readbyqxmd.com/read/28892930/the-impact-of-three-month-training-programme-on-foot-care-and-self-efficacy-of-patients-with-diabetic-foot-ulcers
#2
Raziyeh Sadat Bahador, Seyedeh Sara Afrazandeh, Nezar Ghanbarzehi, Maryam Ebrahimi
INTRODUCTION: Patient's self-efficacy in disease management and foot care is considered as an important indicator in controlling the complications of diabetes. AIM: This study was aimed to determine the effect of three-month training programme on foot care and self-efficacy of patients with diabetic foot ulcers. MATERIALS AND METHODS: A quasi-experimental study was conducted on 60 patients with diabetic foot ulcers in Jiroft Imam Khomeini hospital from January 2016 to May 2016...
July 2017: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/28867453/the-effect-of-indocyanine-green-loaded-on-a-novel-nano-graphene-oxide-for-high-performance-of-photodynamic-therapy-against-enterococcus-faecalis
#3
Tayebeh Akbari, Maryam Pourhajibagher, Farzaneh Hosseini, Nasim Chiniforush, Elham Gholibegloo, Mehdi Khoobi, Sima Shahabi, Abbas Bahador
BACKGROUND: Recently developed photodynamic therapy (PDT) has gained attention for achieving effective root canal disinfection. Using an optimized nontoxic photosensitizer (PS), such as indocyanine green (ICG), is an imperative part of this technique. Therefore, the objective of the current study was to improve ICG photodynamic properties through incorporation of ICG into nano-graphene oxide (NGO) in order to produce NGO-ICG as a new PS and also to assess the antimicrobial effects of NGO-ICG against Enterococcus faecalis after photodynamic therapy...
August 31, 2017: Photodiagnosis and Photodynamic Therapy
https://www.readbyqxmd.com/read/28857113/myofibrils-in-cardiomyocytes-tend-to-assemble-along-the-maximal-principle-stress-directions
#4
Hongyan Yuan, Bahador Marzban, Kevin Kit Parker
The mechanisms underlying the spatial organization of self-assembled myofibrils in cardiac tissues remain incompletely understood. By modeling cells as elastic solids under active cytoskeletal contraction, we found a good correlation between the predicted maximal principal stress directions and the in vitro myofibril orientations in individual cardiomyocytes. This implies that actomyosin fibers tend to assemble along the maximal tensile stress directions. By considering the dynamics of focal adhesion and myofibril formation in the model, we showed that the different patterns of myofibril organizations in mature versus immature cardiomyocytes can be explained as the consequence of the different levels of force-dependent remodeling of focal adhesions...
August 31, 2017: Journal of Biomechanical Engineering
https://www.readbyqxmd.com/read/28797828/gene-expression-profiling-of-fima-gene-encoding-fimbriae-among-clinical-isolates-of-porphyromonas-gingivalis-in-response-to-photo-activated-disinfection-therapy
#5
Maryam Pourhajibagher, Abbas Bahador
BACKGROUND: The endodontic infection therapy majorly aims to eradicate the microbial pathogens. Photo-activated disinfection (PAD), also known as antimicrobial photodynamic therapy, is an alternative antimicrobial modality used to control the microorganisms causing endodontic infections. Notably, microorganisms may be exposed to sub-lethal doses of PAD (sPAD), influencing microbial virulence. The present study assessed the effects of sPAD on expression profiling of the gene associated with the biofilm formation being the most essential virulence factor in Porphyromonas gingivalis strain cells that survive the photodynamic reatment in vivo...
August 7, 2017: Photodiagnosis and Photodynamic Therapy
https://www.readbyqxmd.com/read/28765624/metacognitive-deficiency-in-a-perceptual-but-not-a-memory-task-in-methadone-maintenance-patients
#6
Saeedeh Sadeghi, Hamed Ekhtiari, Bahador Bahrami, Majid Nili Ahmadabadi
Drug addiction has been associated with lack of insight into one's own abilities. However, the scope of metacognition impairment among drug users in general and opiate dependent individuals in particular is not fully understood. Investigating the impairments of metacognitive ability in Substance Dependent Individuals (SDIs) in different cognitive tasks could contribute to the ongoing debate over whether metacognition has domain-general or domain-specific neural substrates. We compared metacognitive self-monitoring ability of a group of SDIs during methadone maintenance treatment (n = 23) with a control group (n = 24) in a memory and a visual perceptual task...
August 1, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28764235/immunodiagnosis-of-human-fascioliasis-an-update-of-concepts-and-performances-of-the-serological-assays
#7
REVIEW
Bahador Sarkari, Samaneh Abdolahi Khabisi
Human Fascioliasis (HF) is a foodborne neglected parasitic disease caused by Fasciola hepatica and Fasciola gigantica. New epidemiological data suggest that the endemic areas of the disease are expanding and HF is being reported from areas where it was previously not observed. Diagnosis of HF is challenging. Performances of parasitological approaches, based on the detection of parasite's egg in the stool, are not satisfactory. Currently serological methods for the diagnosis of HF are mainly based on detection of anti-Fasciola antibodies in serum...
June 2017: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/28761484/comparison-of-three-different-dna-extraction-methods-for-linguatula-serrata-as-a-food-born-pathogen
#8
Gilda Eslami, Sepideh Khalatbari-Limaki, Mohammad Hasan Ehrampoush, Mostafa Gholamrezaei, Bahador Hajimohammadi, Ahmad Oryan
BACKGROUND: One of the most important items in molecular characterization of food-borne pathogens is high quality genomic DNA. In this study, we investigated three protocols and compared their simplicity, duration and costs for extracting genomic DNA from Linguatula serrata. METHODS: The larvae were collected from the sheep's visceral organs from the Yazd Slaughterhouse during May 2013. DNA extraction was done in three different methods, including commercial DNA extraction kit, Phenol Chloroform Isoamylalcohol (PCI), and salting out...
April 2017: Iranian Journal of Parasitology
https://www.readbyqxmd.com/read/28720314/antibiotic-susceptibility-of-helicobacter-pylori-strains-isolated-from-iranian-children-high-frequency-of-a2143g-point-mutation-associated-with-clarithromycin-resistance
#9
Shima Mahmoudi, Setareh Mamishi, Maryam Banar, Sepideh Keshavarz Valian, Abbas Bahador, Mehri Najafi, Fatemeh Farahmand, Babak Pourakbari
OBJECTIVE: Increasing antibiotic resistance in Helicobacter pylori is a global concern and is associated with treatment failure. The aim of this study was to evaluate antibiotic resistance of H. pylori to different antibiotics including amoxicillin, erythromycin, ciprofloxacin, furazolidone, tetracycline, metronidazole and clarithromycin. In addition, ​detection of A2143G mutation in clarithromycin resistant isolates was performed using real-time PCR technique. METHODS: Ninety patients with upper gastrointestinal symptoms were enrolled in this study...
September 2017: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/28707264/evaluation-of-propylene-glycol-nanoliposomes-containing-curcumin-on-burn-wound-model-in-rat-biocompatibility-wound-healing-and-anti-bacterial-effects
#10
Nooshin Kianvash, Abbas Bahador, Maryam Pourhajibagher, Homanaz Ghafari, Vahid Nikoui, Sayed Mehdi Rezayat, Ahmad Reza Dehpour, Alireza Partoazar
Curcumin is an effective wound healing agent in burn therapy, but due to its low bioavailability, it is required to be formulated for topical therapy. Liposomal nanocarriers are developed as stable and efficient dermal delivery systems. In this study, we prepared curcumin-propylene glycol liposomes (Cur-PgL) to treat animals subjected to second degree burns. The characterization tests confirmed the production of monodisperse nanoliposomes of average size of about 145 nm with high entrapment efficiency percentage and a sustained release behavior...
July 13, 2017: Drug Delivery and Translational Research
https://www.readbyqxmd.com/read/28645784/the-effect-of-antimicrobial-photodynamic-therapy-on-the-expression-of-novel-methicillin-resistance-markers-determined-using-cdna-aflp-approach-in-staphylococcus-aureus
#11
Mohammad Neshvan Hoorijani, Hosein Rostami, Maryam Pourhajibagher, Nasim Chiniforush, Mansour Heidari, Babak Pourakbari, Hossein Kazemian, Kambiz Davari, Vahid Amini, Reza Raoofian, Abbas Bahador
BACKGROUND: Widespread methicillin resistant Staphylococcus aureus (MRSA) and absence of effective antimicrobial agents has led to limited therapeutic options for treating MRSA infection. We aimed to evaluate the effect of antimicrobial photodynamic therapy (aPDT) on the expression of novel identified methicillin resistance markers (NIMRMs) in S. aureus using complementary DNA-Amplified Fragment Length Polymorphism (cDNA-AFLP) approaches to address the therapeutic alternatives for MRSA infections...
September 2017: Photodiagnosis and Photodynamic Therapy
https://www.readbyqxmd.com/read/28630383/balb-c-mice-immunity-to-hydatidosis-induced-by-in-vitro-reared-echinococcus-granulosus-adult-worm-antigens
#12
Hamid Reza Rahimi, Tahereh Mohammadzadeh, Seyed Mahmoud Sadjjadi, Bahador Sarkari, Farzaneh Zahabiun
BACKGROUND: Echinococcosis is a zoonotic parasitic disease caused by the larval stage of Echinococcus granulosus. Several native and recombinant antigens, derived from different stages of E. granulosus life cycle, have been used for vaccine trials. In vitro reared adult worms are good candidates for vaccination as they do not produce fertile egg/s and do not have any risk of contamination for researchers. OBJECTIVE: To evaluate different antigens derived from in vitro reared E...
June 2017: Iranian Journal of Immunology: IJI
https://www.readbyqxmd.com/read/28630382/immunodiagnostic-value-of-echinococcus-granulosus-recombinant-b8-1-subunit-of-antigen-b
#13
Amir Savardashtaki, Bahador Sarkari, Farzane Arianfar, Zohreh Mostafavi-Pour
BACKGROUND: Cystic echinococcosis (CE), as a chronic parasitic disease, is a major health problem in many countries. The performance of the currently available serodiagnostic tests for the diagnosis of CE is unsatisfactory. OBJECTIVE: The current study aimed at sub-cloning a gene, encoding the B8/1 subunit of antigen B (AgB) from Echinococcus granulosus, using gene optimization for the immunodiagnosis of human CE. METHODS: The coding sequence for AgB8/1 subunit of Echinococcus granulosus was selected from GenBank and was gene-optimized...
June 2017: Iranian Journal of Immunology: IJI
https://www.readbyqxmd.com/read/28489857/vitamin-d-supplementation-lowers-thrombospondin-1-levels-and-blood-pressure-in-healthy-adults
#14
Anjalee T Amarasekera, Bahador Assadi-Khansari, Saifei Liu, Marilyn Black, Greer Dymmott, Natasha M Rogers, Aaron L Sverdlov, John D Horowitz, Doan T M Ngo
INTRODUCTION: Vitamin D insufficiency, defined as 25-hydroxyvitamin D (25(OH)D) levels < 75nmol/L is associated with cardio-metabolic dysfunction. Vitamin D insufficiency is associated with inflammation and fibrosis, but it remains uncertain whether these anomalies are readily reversible. Therefore, we aimed to determine the effects of vitamin D supplementation on markers of: 1) nitric oxide (NO) signaling, 2) inflammation, and 3) fibrosis, in healthy volunteers with mild hypovitaminosis...
2017: PloS One
https://www.readbyqxmd.com/read/28485708/molecular-study-of-carbapenemase-genes-in-clinical-isolates-of-enterobacteriaceae-resistant-to-carbapenems-and-determining-their-clonal-relationship-using-pulsed-field-gel-electrophoresis
#15
Fereshteh Shahcheraghi, Mohammad Mehdi Aslani, Hassan Mahmoudi, Zahra Karimitabar, Hamid Solgi, Abbas Bahador, Mohammad Yousef Alikhani
PURPOSE: Enterobacteriaceae is a large family of Gram-negative bacteria that are considered as normal gut flora. They are the most common human pathogens. The main objective of this study was to investigate the carbapenemase genes in clinical isolates of Enterobacteriaceae resistant to carbapenem antibiotics and determine their clonal relationship using pulsed-field gel electrophoresis (PFGE). METHODOLOGY: In the present study, bacteria were isolated and identified via conventional biochemical tests and API 20NE...
May 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28450261/monitoring-gene-expression-of-rcpa-from-aggregatibacter-actinomycetemcomitans-versus-antimicrobial-photodynamic-therapy-by-relative-quantitative-real-time-pcr
#16
Maryam Pourhajibagher, Nasim Chiniforush, Sima Shahabi, Shaghayegh Sobhani, Mohammad Moein Monzavi, Abbas Monzavi, Abbas Bahador
BACKGROUND: Aggregatibacter actinomycetemcomitans is an important pathogen that is frequently found in various infections, particularly aggressive periodontitis. In this study, we described the outcome of the expression level of A. actinomycetemcomitans virulence factor following treatment by antimicrobial photodynamic therapy (aPDT) with indocyanine green (ICG) as a photosensitizing agent. MATERIALS AND METHODS: To determine the aPDT effect on the cell-surviving assay and expression ratio of the rcpA gene in A...
September 2017: Photodiagnosis and Photodynamic Therapy
https://www.readbyqxmd.com/read/28448443/the-effect-of-thermal-fluctuation-on-the-receptor-mediated-adhesion-of-a-cell-membrane-to-an-elastic-substrate
#17
Bahador Marzban, Hongyan Yuan
Mechanics of the bilayer membrane play an important role in many biological and bioengineering problems such as cell-substrate and cell-nanomaterial interactions. In this work, we study the effect of thermal fluctuation and the substrate elasticity on the cell membrane-substrate adhesion. We model the adhesion of a fluctuating membrane on an elastic substrate as a two-step reaction comprised of the out-of-plane membrane fluctuation and the receptor-ligand binding. The equilibrium closed bond ratio as a function of substrate rigidity was computed by developing a coupled Fourier space Brownian dynamics and Monte Carlo method...
April 27, 2017: Membranes
https://www.readbyqxmd.com/read/28438509/effect-of-photodynamic-therapy-based-on-indocyanine-green-on-expression-of-apoptosis-related-genes-in-human-gingival-fibroblast-cells
#18
Samira Gharesi, Maryam Pourhajibagher, Nasim Chiniforush, Reza Raoofian, Mehrdad Hashemi, Sima Shahabi, Abbas Bahador
BACKGROUND: Periodontal diseases refer to inflammation of the gingiva, induction of apoptosis in human gingival fibroblast cells, destruction of the surrounding tissues, and early bone loss resulting in infections due to the pathogenic activity of the microorganisms and the host immune inflammatory responses. Recent investigations have suggested that antimicrobial photodynamic therapy (aPDT) can be an adjunct treatment therapy for periodontal infections. AIM: To prove the lack of side effects of PDT on periodontal tissues, we investigated the expression of BAX and BCL-2 genes that are involved in apoptosis after the PDT on human gingival fibroblast (HGF) cells...
September 2017: Photodiagnosis and Photodynamic Therapy
https://www.readbyqxmd.com/read/28430524/mirna-related-polymorphisms-in-mir-423-rs6505162-and-pex6-rs1129186-and-risk-of-esophageal-squamous-cell-carcinoma-in-an-iranian-cohort
#19
Ziba Nariman-Saleh-Fam, Milad Bastami, Mohammad Hossein Somi, Farkhondeh Behjati, Yaser Mansoori, Abdolreza Daraei, Zahra Saadatian, Lida Nariman-Saleh-Fam, Habibollah Mahmoodzadeh, Yashar Makhdoumi, Fatemeh Varshoee Tabrizi, Bahador Ebrahimi-Sharif, Azam Hezarian, Shahnaz Naghashi, Mohammad Reza Abbaszadegan, Javad Tavakkoly-Bazzaz
AIMS: Iran is located in the Asian esophageal cancer belt. It is a high-risk region for esophageal squamous cell carcinoma (ESCC). The extent to which genetic components, especially variants within miRNAs or their binding sites, contribute to risk of ESCC in the region is not yet fully understood. Herein, tests were done on an Iranian cohort to evaluate the association of miRNA-related polymorphisms in miR-423 (rs6505162) and peroxisomal biogenesis factor 6 (PEX6) (rs1129186 within a miR-149-5p-binding site) with the risk of ESCC risk...
June 2017: Genetic Testing and Molecular Biomarkers
https://www.readbyqxmd.com/read/28415483/an-investigation-of-electrospun-henna-leaves-extract-loaded-chitosan-based-nanofibrous-mats-for-skin-tissue-engineering
#20
Iman Yousefi, Mehdi Pakravan, Hoda Rahimi, Abbas Bahador, Zahra Farshadzadeh, Ismael Haririan
Wound healing characteristics of some plant extracts have been well known for many years, and they have been utilized for such applications in traditional way. Recently electrospun nanofibrous mats showed promising properties for tissue engineering and especially for skin repair. It is expected that incorporation of plant extracts into such structures could provide higher performance and synergistic effect for biomedical and wound healing applications. The final purpose of this study is to fabricate chitosan based nanofiber mats loaded with a traditional plant extract of Lawsonia inermis (Henna) leaves to enhance the antibacterial efficacy and wound healing of the precursor nanofibers...
June 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
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