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https://www.readbyqxmd.com/read/28935988/a-smart-thermo-and-ph-responsive-microfiltration-membrane-based-on-three-dimensional-inverse-colloidal-crystals
#1
Bing Yu, Qianqian Song, Hailin Cong, Xiaodan Xu, Dongwei Han, Zhongmin Geng, Xiaoyan Zhang, Muhammad Usman
In this paper, a thermo- and pH-responsive microfiltration membrane was prepared based on three-dimensional (3D) inverse colloidal crystals (ICC). To manufacture the smart ICC membrane, the typical thermo-responsive N-isopropylacrylamide (NIPAM) and pH-responsive methacrylic acid (MAA) were polymerized inside silica colloidal crystals. The smart ICC membranes were characterized by SEM, IR and contact angle measurements. Moreover, the permeability of smart microfiltration membrane was carried out by the KCl diffusion tests...
September 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28934276/enhanced-pyruvate-dehydrogenase-activity-improves-cardiac-outcomes-in-a-murine-model-of-cardiac-arrest
#2
Lin Piao, Yong-Hu Fang, Manfred M Kubler, Michael W Donnino, Willard W Sharp
RATIONALE: Post-ischemic changes in cellular metabolism alter myocardial and neurological function. Pyruvate dehydrogenase (PDH), the limiting step in mitochondrial glucose oxidation, is inhibited by increased expression of PDH kinase (PDK) during ischemia/reperfusion injury. This results in decreased utilization of glucose to generate cellular ATP. Post-cardiac arrest (CA) hypothermia improves outcomes and alters metabolism, but its influence on PDH and PDK activity following CA are unknown...
2017: PloS One
https://www.readbyqxmd.com/read/28931751/essential-role-of-kir5-1-channels-in-renal-salt-handling-and-blood-pressure-control
#3
Oleg Palygin, Vladislav Levchenko, Daria V Ilatovskaya, Tengis S Pavlov, Oleh M Pochynyuk, Howard J Jacob, Aron M Geurts, Matthew R Hodges, Alexander Staruschenko
Supplementing diets with high potassium helps reduce hypertension in humans. Inwardly rectifying K+ channels Kir4.1 (Kcnj10) and Kir5.1 (Kcnj16) are highly expressed in the basolateral membrane of distal renal tubules and contribute to Na+ reabsorption and K+ secretion through the direct control of transepithelial voltage. To define the importance of Kir5.1 in blood pressure control under conditions of salt-induced hypertension, we generated a Kcnj16 knockout in Dahl salt-sensitive (SS) rats (SSKcnj16-/-). SSKcnj16-/- rats exhibited hypokalemia and reduced blood pressure, and when fed a high-salt diet (4% NaCl), experienced 100% mortality within a few days triggered by salt wasting and severe hypokalemia...
September 21, 2017: JCI Insight
https://www.readbyqxmd.com/read/28930433/high-resolution-graphene-films-for-electrochemical-sensing-via-inkjet-maskless-lithography
#4
John A Hondred, Loreen R Stromberg, Curtis L Mosher, Jonathan C Claussen
Solution-phase printing of nanomaterial-based graphene inks are rapidly gaining interest for fabrication of flexible electronics. However, scalable manufacturing techniques for high-resolution printed graphene circuits are still lacking. Here, we report a patterning technique [i.e., inkjet maskless lithography (IML)] to form high resolution, flexible, graphene films (line widths down to 20 µm) that significantly exceeds the current inkjet printing resolution of graphene (line widths ~ 60 µm). IML uses an inkjet printed polymer lacquer as a sacrificial pattern, viscous spin coated graphene, and a subsequent graphene lift-off to patterned films without the need for pre-fabricated stencils, templates or cleanroom technology (e...
September 20, 2017: ACS Nano
https://www.readbyqxmd.com/read/28930339/controlled-electrochemical-doping-of-graphene-based-3d-nanoarchitecture-electrodes-for-supercapacitors-and-capacitive-deionisation
#5
A M Abdelkader, D J Fray
Chemically-doped graphenes are promising electrode materials for energy storage and electrosorption applications. Here, an affordable electrochemical green process is introduced to dope graphene with nitrogen. The process is based on reversing the polarity of two identical graphene oxide (GO) electrodes in molten KCl-LiCl-Li3N. During the cathodic step, the oxygen functional groups on the GO surface are removed through direct electro-deoxidation reactions or a reaction with the deposited lithium. In the anodic step, nitrogen is adsorbed onto the surface of graphene and subsequently reacts to form nitrogen-doped graphene...
September 20, 2017: Nanoscale
https://www.readbyqxmd.com/read/28929535/extracellular-acidosis-selectively-inhibits-pharmacomechanical-coupling-induced-by-carbachol-in-strips-of-rat-gastric-fundus
#6
Daniel Maia Nogueira de Oliveira, Francisco José Batista-Lima, Emanuella Carvalho Feitosa, Alexandre Havt, Moisés Tolentino Bento da Silva, Armênio Aguiar Dos Santos, Pedro Jorge Caldas Magalhães
Acute acidosis that results from short-term exercise delays gastric emptying in rats and decreases the responsiveness to carbachol in gastric fundus strips. The regulation of cytosolic Ca(2+) levels appears to be a mechanism of action of acidosis. The present study investigated the way in which acidosis interferes with gastric smooth muscle contractions. Rat gastric fundus isolated strips at pH 6.0 presented a lower magnitude of carbachol-induced contractions compared with preparations at pH 7.4. This lower magnitude was absent in carbachol-stimulated duodenum and KCl-stimulated gastric fundus strips...
September 19, 2017: Experimental Physiology
https://www.readbyqxmd.com/read/28928787/the-effect-of-schinus-terebinthifolius-raddi-anacardiaceae-bark-extract-on-histamine-induced-paw-edema-and-ileum-smooth-muscle-contraction
#7
Paulo Alexandre Nunes-Neto, Tadeu José da Silva Peixoto-Sobrinho, Edilson Dantas da Silva Júnior, Jamilka Leopoldina da Silva, Alisson Rodrigo da Silva Oliveira, André Sampaio Pupo, Alice Valença Araújo, João Henrique da Costa-Silva, Almir Gonçalves Wanderley
Schinus terebinthifolius Raddi (Anacardiaceae), popularly known as red aroeira, is used in traditional medicine to treat inflammatory, gastric, and respiratory disorders. The aim of this study was to evaluate the antihistaminic activity of S. terebinthifolius (St) bark extract by using in vivo and in vitro experimental models. The effects of St were investigated on contractions induced by histamine, carbachol, and potassium chloride in isolated guinea pig ileum. St was also studied in response to hind paw edema induced by histamine in rats...
2017: Evidence-based Complementary and Alternative Medicine: ECAM
https://www.readbyqxmd.com/read/28926857/the-amelioration-of-insulin-resistance-in-salt-loading-subjects-by-potassium-supplementation-is-associated-with-a-reduction-in-plasma-il-17a-levels
#8
Wen Wen, Zhaofei Wan, Dong Zhou, Juan Zhou, Zuyi Yuan
Background High dietary salt intake contributes to the development of autoimmune/inflammatory diseases including metabolic syndrome (MetS) which potassium supplementation can potentially reverse. T helper (Th) 17 cells as well as its production interleukin (IL)-17A are involved in the pathogenesis of MetS. The polarization of Th17 cells and enhanced IL-17A production induced by high salt might increase the risk of autoimmune/inflammatory diseases. Methods 45 normotensive subjects (aged 29 to 65 years) were enrolled from a rural community of Northern China at random...
September 2017: Experimental and Clinical Endocrinology & Diabetes
https://www.readbyqxmd.com/read/28926039/tuning-linkage-isomerism-and-magnetic-properties-of-bi-and-tri-metallic-cage-silsesquioxanes-by-cation-and-solvent-effects
#9
Alexey N Bilyachenko, Alexander A Korlyukov, Anna V Vologzhanina, Victor N Khrustalev, Alena N Kulakova, Jérôme Long, Joulia Larionova, Yannick Guari, Marina S Dronova, Ulyana S Tsareva, Pavel V Dorovatovskii, Elena S Shubina, Mikhail M Levitsky
Herein, the effect of replacement of the surrounding solvent and/or the partial substitution of sodium ions in the cage-like copper, sodium phenylsilsesquioxane [(PhSiO1.5)12(CuO)4(NaO0.5)4(n-BuOH)6] 1 with a globular structure was investigated; the synthesis of ten new derivatives of complex 1 was performed, and their crystal structures were determined. Solvate replacement of n-butanol in 1 with dimethyl sulfoxide, acetonitrile, 1,4-dioxane/EtOH, and 1,4-dioxane/PhCN afforded the complexes [(PhSiO1.5)12(CuO)4(NaO0...
September 19, 2017: Dalton Transactions: An International Journal of Inorganic Chemistry
https://www.readbyqxmd.com/read/28922508/single-oxidative-collision-events-of-silver-nanoparticles-understanding-the-rate-determining-chemistry
#10
Kamonwad Ngamchuea, Richard O D Clark, Stanislav V Sokolov, Neil P Young, Christopher Batchelor-McAuley, Richard G Compton
The oxidative dissolution of citrate-capped silver nanoparticles (AgNPs) is investigated herein by two electrochemical techniques: nano-impacts and anodic stripping voltammetry. Nano-impacts or single nanoparticle-electrode collisions allow the detection of individual nanoparticles. The technique offers an advantage over surface-immobilized methods such as anodic stripping voltammetry as it eliminates the effects of particle agglomeration/ aggregation. The electrochemical studies are performed in different electrolytes (KNO3, KCl, KBr and KI) at varied concentrations ( 20 mM)...
September 18, 2017: Chemistry: a European Journal
https://www.readbyqxmd.com/read/28916033/ca-2-detection-utilising-algan-gan-transistors-with-ion-selective-polymer-membranes
#11
Mohsen Asadnia, Matthew Myers, Gilberto A Umana-Membreno, Tarun M Sanders, Umesh K Mishra, Brett D Nener, Murray V Baker, Giacinta Parish
We demonstrate highly selective and sensitive potentiometric ion sensors for calcium ion detection, operated without the use of a reference electrode. The sensors consist of AlGaN/GaN heterostructure-based transistor devices with chemical functionalisation of the gate area using poly (vinylchloride)-based (PVC) membranes having high selectivity towards calcium ions, Ca(2+). The sensors exhibited stable and rapid responses when introduced to various concentrations of Ca(2+). In both 0.01 M KCl and 0.01 M NaCl ionic strength buffer solutions, the sensors exhibited near Nernstian responses with detection limits of less than 10(-7) M, and a linear response range between 10(-7)-10(-2) M...
September 22, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28912106/vascular-activation-of-k-channels-and-na-k-atpase-activity-of-estrogen-deficient-female-rats
#12
Rogério Faustino Ribeiro, Jonaina Fiorim, Vinicius Bermond Marques, Karoline de Sousa Ronconi, Tatiani Botelho, Marcella D Grando, Lusiane M Bendhack, Dalton Valentim Vassallo, Ivanita Stefanon
The goal of the present study was to evaluate vascular potassium channels and Na(+)-K(+)-ATPase activity in estrogen deficient female rats. Female rats that underwent ovariectomy were assigned to receive daily treatment with placebo (OVX) or estrogen replacement (OVX+E2, 1mg/kg, once a week, i.m.). Aortic rings were used to examine the involvement of K(+) channels and Na(+)-K(+)-ATPase in vascular reactivity. Acetylcholine (ACh)-induced relaxation was analyzed in the presence of L-NAME (100μM) and K(+) channels blockers: tetraethylammonium (TEA, 5mM), 4-aminopyridine (4-AP, 5mM), iberiotoxin (IbTX, 30nM), apamin (0...
September 11, 2017: Vascular Pharmacology
https://www.readbyqxmd.com/read/28911118/multimerization-rules-for-g-quadruplexes
#13
Sofia Kolesnikova, Martin Hubálek, Lucie Bednárová, Josef Cvacka, Edward A Curtis
G-quadruplexes can multimerize under certain conditions, but the sequence requirements of such structures are not well understood. In this study, we investigated the ability of all possible variants of the central tetrad in a monomeric, parallel-strand G-quadruplex to form higher-order structures. Although most of these 256 variants existed primarily as monomers under the conditions of our screen, ∼10% formed dimers or tetramers. These structures could form in a wide range of monovalent and divalent metal ions, and folding was highly cooperative in both KCl and MgCl2...
September 6, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/28905087/a-droplet-based-microfluidic-chip-as-a-platform-for-leukemia-cell-lysate-identification-using-surface-enhanced-raman-scattering
#14
Mohamed Hassoun, Jan Rüger, Tatiana Kirchberger-Tolstik, Iwan W Schie, Thomas Henkel, Karina Weber, Dana Cialla-May, Christoph Krafft, Jürgen Popp
A new approach is presented for cell lysate identification which uses SERS-active silver nanoparticles and a droplet-based microfluidic chip. Eighty-nanoliter droplets are generated by injecting silver nanoparticles, KCl as aggregation agent, and cell lysate containing cell constituents, such as nucleic acids, carbohydrates, metabolites, and proteins into a continuous flow of mineral oil. This platform enables accurate mixing of small volumes inside the meandering channels of the quartz chip and allows acquisition of thousands of SERS spectra with 785 nm excitation at an integration time of 1 s...
September 13, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28902504/synthesis-of-altrose-poly-amido-saccharides-with-%C3%AE-n-1%C3%A2-2-d-amide-linkages-a-right-handed-helical-conformation-engineered-in-at-the-monomer-level
#15
Ruiqing Xiao, Eric L Dane, Jialiu Zeng, Christopher James McKnight, Mark W Grinstaff
The design and synthesis of amide-linked saccharide oligomers and polymers, which are predisposed to fold into specific ordered secondary structures, is of significant interest. Herein, right-handed helical poly-amido-saccharides (PASs) with β-N-(1→2)-D-amide linkages are synthesized by the anionic ring-opening polymerization of an altrose β-lactam monomer (alt-lactam). The right-handed helical conformation is engineered into the polymer by pre-installing the β configuration of the lactam ring in the monomer via the stereospecific [2+2] cycloaddition of trichloroacetyl isocyanate with a D-glycal possessing a 3-benzyloxyl group oriented to the α-face of the pyranose...
September 13, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/28902447/synthesis-of-monodisperse-silica-microspheres-and-modification-with-diazoresin-for-mixed-mode-ultra-high-performance-liquid-chromatography-separations
#16
Hailin Cong, Bing Yu, Chao Tian, Shuai Zhang, Hua Yuan
Monodisperse silica particles with average diameters of 1.9-2.9 μm were synthesized by a modified Stöber method, in which tetraethyl orthosilicate was continuously supplied to the reaction mixture containing KCl electrolyte, water, ethanol and ammonia. The obtained silica particles were modified by self-assembly with positively charged photosensitive diazoresin on the surface. After treatment with ultraviolet light, the ionic bonding between silica and diazoresin was converted into covalent bonding through a unique photochemistry reaction of diazoresin...
September 13, 2017: Journal of Separation Science
https://www.readbyqxmd.com/read/28900594/adsorption-and-electronic-properties-of-pentacene-on-thin-dielectric-decoupling-layers
#17
Sebastian Koslowski, Daniel Rosenblatt, Alexander Kabakchiev, Klaus Kuhnke, Klaus Kern, Uta Schlickum
With the increasing use of thin dielectric decoupling layers to study the electronic properties of organic molecules on metal surfaces, comparative studies are needed in order to generalize findings and formulate practical rules. In this paper we study the adsorption and electronic properties of pentacene deposited onto h-BN/Rh(111) and compare them with those of pentacene deposited onto KCl on various metal surfaces. When deposited onto KCl, the HOMO and LUMO energies of the pentacene molecules scale with the work functions of the combined KCl/metal surface...
2017: Beilstein Journal of Nanotechnology
https://www.readbyqxmd.com/read/28895737/migration-of-phospholipid-vesicles-can-be-selectively-driven-by-concentration-gradients-of-metal-chloride-solutions
#18
Atsuji Kodama, Yuka Sakuma, Masayuki Imai, Toshihiro Kawakatsu, Nicolas Puff, Miglena I Angelova
We have investigated migrations of phospholipid vesicles under the concentration gradients of metal ions. We micro-injected metal chloride solutions, monovalent (NaCl and KCl), divalent (CaCl2 and MgCl2), and trivalent (LaCl3) salts, toward phospholipid giant vesicles (GVs) composed of 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC). For NaCl, CaCl2, and MgCl2 solutions, the GVs migrated straight toward the tip of the micro-pipette in response to the concentration gradients, whereas for KCl and LaCl3, GVs moved to the opposite direction...
September 12, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28893640/agmatine-attenuates-intestinal-ischemia-and-reperfusion-injury-by-reducing-oxidative-stress-and-inflammatory-reaction-in-rats
#19
Inci Turan, Hale Sayan Ozacmak, V Haktan Ozacmak, Figen Barut, Mehmet Araslı
AIMS: Oxidative stress and inflammatory response are major factors causing several tissue injuries in intestinal ischemia and reperfusion (I/R). Agmatine has been reported to attenuate I/R injury of various organs. The present study aims to analyze the possible protective effects of agmatine on intestinal I/R injury in rats. MAIN METHODS: Four groups were designed: sham control, agmatine-treated control, I/R control, and agmatine-treated I/R groups. IR injury of small intestine was induced by the occlusion of the superior mesenteric artery for half an hour to be followed by a 3-hour-long reperfusion...
September 8, 2017: Life Sciences
https://www.readbyqxmd.com/read/28889056/voltammetric-behavior-of-mammeisin-ma-at-a-glassy-carbon-electrode-and-its-interaction-with-bovine-serum-albumin-bsa
#20
Jules-Blaise Mabou Leuna, Sergeot Kungo Sop, Suzanne Makota, Evangeline Njanja, Thiery Christophe Ebelle, Anatole Guy Azebaze, Emmanuel Ngameni, Achille Nassi
The electrochemical oxidation of Mammeisin (MA) was studied in a solution containing acetone and 0.1M phosphate buffer +0.1M KCl (pH=5.3) at a glassy carbon electrode (GCE), using cyclic (CV) and square wave voltammetry (SWV). MA showed a quasi-reversible process, which is pH dependent and that involves the exchange of two electrons and two protons. The oxidation product was adsorbed by the electrode surface to form a film that blocks active sites over repetitive cyclic. Moreover, the interaction of MA and bovine serum albumin (BSA) was studied by CV and SWV at different pHs (5...
September 7, 2017: Bioelectrochemistry
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