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https://www.readbyqxmd.com/read/28340454/pea-cultivar-and-wheat-residues-affect-carbon-nitrogen-dynamics-in-pea-triticale-intercropping-a-microcosms-approach
#1
Antonella Scalise, Valentini A Pappa, Antonio Gelsomino, Robert M Rees
The underlying mechanisms by which legume cultivars contribute to nitrous oxide (N2O) generation are poorly understood. The aim of the present study was to explore the effects of two pea cultivars (Zero4 and Nitouche) intercropped with triticale, with or without wheat (Triticum aestivum) residues incorporation, on soil C and N dynamics, on bacterial community structure and their links with N2O emissions. Monocrops and bare soil (no plant) treatments were used as an additional control in order to account for the level of mineralisation between treatments...
March 21, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28336814/airway-smooth-muscle-dysfunction-in-pompe-gaa-mice
#2
Allison M Keeler, Donghai Liu, Marina Zieger, Lang Xiong, Jeffrey Salemi, Karl Bellve, Barry J Byrne, David D Fuller, Ronghua ZhuGe, Mai K ElMallah
Pompe disease is an autosomal recessive disorder caused by a deficiency of acid alpha-glucosidase (GAA) - an enzyme responsible for hydrolyzing lysosomal glycogen. Deficiency of GAA leads to systemic glycogen accumulation in the lysosomes of skeletal muscle, motor neurons and smooth muscle. Skeletal muscle and motor neuron pathology are known to contribute to respiratory insufficiency in Pompe disease, but the role of airway pathology has not been evaluated. Here we propose that GAA enzyme deficiency disrupts the function of the trachea and bronchi, and this lower airway pathology contributes to respiratory insufficiency in Pompe disease...
March 23, 2017: American Journal of Physiology. Lung Cellular and Molecular Physiology
https://www.readbyqxmd.com/read/28333147/solute-carrier-family-12-member-5-promotes-tumor-invasion-metastasis-of-bladder-urothelial-carcinoma-by-enhancing-nf-%C3%AE%C2%BAb-mmp-7-signaling-pathway
#3
J Y Liu, Y B Dai, X Li, K Cao, D Xie, Z T Tong, Z Long, H Xiao, M K Chen, Y L Ye, B Liu, J Tan, J Tang, Z Z Xu, Y Gan, Y H Zhou, F Deng, L Y He
Solute carrier family 12 member 5 (SLC12A5), an integral membrane KCl cotransporter, which maintains chloride homeostasis in neurons, is aberrantly expressed and involved in the tumorigenesis of certain cancers. However, the clinical significance and biological role of SLC12A5 in human bladder urothelial carcinoma (BUC) remains unclear. In this study, the expression of SLC12A5 was examined in clinical specimens of primary BUC and in BUC cell lines using quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR), western blot and immunohistochemistry (IHC)...
March 23, 2017: Cell Death & Disease
https://www.readbyqxmd.com/read/28330278/effects-of-the-culture-media-optimization-on-pectinase-production-by-enterobacter-sp-pstb-1
#4
M Purna Chandra Reddy, K V Saritha
In the present study, media composition for high production of pectinase by Enterobacter sp. PSTB-1 in submerged fermentation was optimized using response surface methodology (RSM). Mango fruit processing industrial waste (MIW) was used as substrate (carbon source) as it contains high amount of pectin. Enterobacter sp. PSTB-1 used in present study has given pectin clear zone (PCZ) of 34 mm is higher than other isolates. The experimental results made by statistical design for high pectinase production revealed that the suitable media components: NaNO3 2...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28325558/l-citrulline-ameliorates-cerebral-blood-flow-during-cortical-spreading-depression-in-rats-involvement-of-nitric-oxide-and-prostanoids-mediated-pathway
#5
Yuki Kurauchi, Koichi Mokudai, Asami Mori, Kenji Sakamoto, Tsutomu Nakahara, Masahiko Morita, Ayako Kamimura, Kunio Ishii
l-Citrulline is a potent precursor of l-arginine, and exerts beneficial effect on cardiovascular system via nitric oxide (NO) production. Migraine is one of the most popular neurovascular disorder, and imbalance of cerebral blood flow (CBF) observed in cortical spreading depression (CSD) contributes to the mechanism of migraine aura. Here, we investigated the effect of l-citrulline on cardiovascular changes to KCl-induced CSD. in rats. Intravenous injection of l-citrulline prevented the decrease in CBF, monitored by laser Doppler flowmetry, without affecting mean arterial pressure and heart rate during CSD...
February 17, 2017: Journal of Pharmacological Sciences
https://www.readbyqxmd.com/read/28322207/first-report-of-rahnella-aquatilis-infection-in-crucian-carp-carassius-auratus-in-china
#6
Ai-Jun Lü, Rui-Xia Wang, Xiu-Cai Hu, Jing-Feng Sun, Li Li, Chao Pei, Chao Zhang, Guo-Xing Nie
Rahnella aquatilis infection is rare in aquaculture. Here, a Gram-negative rod-shaped bacterium was isolated from diseased crucian carp Carassius auratus in Xuzhou City, Jiangsu Province, eastern China. The isolate was tentatively named strain KCL-5, and subsequently identified as R. aquatilis by biochemical properties and molecular techniques. The results showed that the isolate KCL-5 was most closely related to the type strain ATCC33071 (= DSM4594) of R. aquatilis, which shared 99.67, 96.26 and 99.58% nucleotide sequence identities for 16S rDNA, gyrB and toxin yhaV genes, respectively...
March 21, 2017: Diseases of Aquatic Organisms
https://www.readbyqxmd.com/read/28315477/magnetic-but-not-non-magnetic-reduced-graphene-oxide-in-spinal-cord-increases-nociceptive-neuronal-responsiveness
#7
Luis Paulo Manzo, Helder Ceragioli, Ivan José Bonet, Catarine Massucato Nishijima, Willians Fernando Vieira, Elaine Conceição Oliveira, João-Batista Destro-Filho, César Renato Sartori, Claudia Herrera Tambeli, Carlos Amilcar Parada
The interference between external magnetic fields and neurophysiology is not new, however, the role of the neuronal magnetic field remains unclear. This study aimed at investigating a possible role of the neuronal magnetic field in nociception. Highly and poorly magnetic reduced graphene oxide (rGO) were injected intrathecally in rats. Nociceptive responsiveness was greater in rats that received highly magnetic-rGO in von Frey electronic or intraplantar capsaicin tests. Furthermore, in vitro experiments demonstrated that the number of KCl-responsive DRG-neurons was greater when treated with highly magnetic-rGO when compared with non-magnetic-rGO...
March 14, 2017: Nanomedicine: Nanotechnology, Biology, and Medicine
https://www.readbyqxmd.com/read/28306357/asthma-causes-inflammation-of-human-pulmonary-arteries-and-decreases-vasodilatation-induced-by-prostaglandin-i2-analogs
#8
Nabil Foudi, Aouatef Badi, Mounira Amrane, Wassim Hodroj
OBJECTIVE: Asthma is a chronic inflammatory disease associated with increased cardiovascular events. This study assesses the presence of inflammation and the vascular reactivity of pulmonary arteries in patients with acute asthma. METHODS: Rings of human pulmonary arteries obtained from non-asthmatic and asthmatic patients were set up in organ bath for vascular tone monitoring. Reactivity was induced by vasoconstrictor and vasodilator agents. Protein expression of inflammatory markers was detected by western blot...
February 21, 2017: Journal of Asthma: Official Journal of the Association for the Care of Asthma
https://www.readbyqxmd.com/read/28304033/separation-of-charge-regulated-polyelectrolytes-by-ph-assisted-diffusiophoresis
#9
Jyh-Ping Hsu, Yen-Rei Hsu, Hsieh Shang-Hung, Shiojenn Tseng
The potential of separating colloidal particles through simultaneous application of a salt gradient and a pH gradient, or pH-assisted diffusiophoresis, is evaluated by considering the case of spherical polyelectrolytes (PEs) having different equilibrium dissociation constants in an aqueous solution with KCl as the background salt. The simulation results gathered reveal that the dependence of the particle velocity on pH is more sensitive than that in pH-assisted electrophoresis, where an electric field and a pH gradient are applied simultaneously...
March 17, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/28300777/functional-contributions-of-positive-charges-in-the-pore-lining-helix-3-of-the-bordetella-pertussis-cyaa-hemolysin-to-hemolytic-activity-and-ion-channel-opening
#10
Chattip Kurehong, Chalermpol Kanchanawarin, Busaba Powthongchin, Panchika Prangkio, Gerd Katzenmeier, Chanan Angsuthanasombat
The Bordetella pertussis CyaA-hemolysin (CyaA-Hly) domain was previously demonstrated to be an important determinant for hemolysis against target erythrocytes and ion-channel formation in planar lipid bilayers (PLBs). Here, net-charge variations in the pore-lining helix of thirteen related RTX cytolysins including CyaA-Hly were revealed by amino acid sequence alignments, reflecting their different degrees of hemolytic activity. To analyze possible functional effects of net-charge alterations on hemolytic activity and channel formation of CyaA-Hly, specific mutations were made at Gln(574) or Glu(581) in its pore-lining α3 of which both residues are highly conserved Lys in the three highly active RTX cytolysins (i...
March 16, 2017: Toxins
https://www.readbyqxmd.com/read/28298357/how-important-is-the-%C3%AE-1-adrenoceptor-in-primate-and-rodent-proximal-urethra-sex-differences-in-the-contribution-of-%C3%AE-1-adrenoceptor-to-urethral-contractility
#11
Eduardo C Alexandre, Mariana G de Oliveira, Rafael Campos, Luiz Ricardo A Kiguti, Fabiano B Calmasini, Fábio H Silva, Andrew D Grant, Naoki Yoshimura, Edson Antunes
Urethral smooth muscle (USM) contributes to urinary continence by contracting during the urine storage phase, which is mainly mediated by activation of post-junctional α1-adrenoceptors. Males and females show differences in the functioning of the lower urinary tract and the most common urinary tract symptoms (LUTS). LUTS in men typically occur in association with bladder outlet obstruction, whereas in women urinary urge-incontinence symptoms are more common. Therefore, this study aimed to evaluate sex differences in α1-adrenoceptor subtype expression and their importance in proximal urethra contraction in mouse (C57BL6/J) and marmoset (Callithrix jacchus)...
March 15, 2017: American Journal of Physiology. Renal Physiology
https://www.readbyqxmd.com/read/28294502/the-role-of-anion-adsorption-in-the-effect-of-electrode-potential-on-spr-response
#12
Veronika K Laurinavichyute, Shavkat Nizamov, Vladimir M Mirsky
Surface plasmon resonance, being widely used in bioanalytics and biotechnology, is influenced by electrical potential of the resonant gold layer. To evaluate the mechanism of this effect, we have studied it in the solution of various inorganic electrolytes. The magnitude of the effect was decreased according to the series: KBr > KCl > KF > NaClO4. The data were treated using different models of the interface. Quantitative description was obtained for the model which takes into account local dielectric function of gold affected by free electron charge, diffuse ionic layer near gold / water interface and specific adsorption of halides to the gold surface with partial charge transfer...
March 11, 2017: Chemphyschem: a European Journal of Chemical Physics and Physical Chemistry
https://www.readbyqxmd.com/read/28290605/transplantation-of-betatrophin-expressing-adipose-derived-mesenchymal-stem-cells-induces-%C3%AE-cell-proliferation-in-diabetic-mice
#13
Liang-Liang Sun, Tian-Jin Liu, Limei Li, Wei Tang, Jun-Jie Zou, Xiang-Fang Chen, Jiao-Yang Zheng, Bei-Ge Jiang, Yong-Quan Shi
Recent progress in regenerative medicine has suggested that mesenchymal stem cell (MSC)-based therapy is a novel potential cure for diabetes. Betatrophin is a newly identified hormone that can increase the production and expansion of insulin-secreting β-cells when administered to mice. In this study, we evaluated the effect of betatrophin overexpression by human adipose-derived MSCs (ADMSCs) by in vitro experiments, as well as following their transplantation into a mice with streptozotocin (STZ)-induced diabetes...
March 10, 2017: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/28290326/emulsions-stabilized-by-high-acyl-gellan-and-kcl
#14
Joice Aline Pires Vilela, Rosiane Lopes da Cunha
Emulsions containing high acyl gellan (0.025 or 0.100% w/w) as emulsifier/stabilizer with KCl addition (1.6-15,633mg of KCl/g of gellan) were evaluated. In general a decrease in apparent viscosity and an increase of the droplets size were observed as KCl content rises. Addition of intermediate KCl concentration (62.6-1,563mg of KCl/g of gellan) could improve the stability to macroscopic phase separation of emulsions with the lower gellan concentration (0.025% w/w). However it was observed that KCl content above a critical value (1,563mg of KCl/g of gellan) caused the breakup of the emulsions containing 0...
January 2017: Food Research International
https://www.readbyqxmd.com/read/28287882/endothelium-independent-vasorelaxant-activity-of-trachyspermum-ammi-essential-oil-on-rat-aorta
#15
G Sargazi Zadeh, N Panahi
Several pharmacological activities of the essential oil of Trachyspermum ammi seeds (TAEO) have been previously studied. These include antitussive, antihypertensive, and antispasmodic effects. However, its action on isolated aorta has not yet been studied. This study was aimed to investigate the vasorelaxant activity of TAEO and characterize its mechanism of action. Extraction of TAEO was performed using Clevenger-type apparatus with the final content of 4.5% (v/w). To evaluate some probable mechanisms of action of TAEO, the action isometric tension was then measured in the aortic rings from Wistar rats which were precontracted with phenylephrine (PHE) (1 µM) or KCl (60 mM)...
2017: Clinical and Experimental Hypertension: CHE
https://www.readbyqxmd.com/read/28286950/diffuse-layer-effect-on-electron-transfer-kinetics-measured-by-scanning-electrochemical-microscopy-secm
#16
Je Hyun Bae, Yun Yu, Michael V Mirkin
Recent theoretical and experimental studies revealed strong effects of the electrical double layer (EDL) on mass transfer at nanometer-sized electrodes and in electrochemical nanogaps. Although the EDL effect is much stronger in weakly supported media, it can significantly influence the kinetics of electron-transfer processes involving multicharged ionic redox species, even at high concentrations of supporting electrolyte. We measured the kinetics of Fe(CN)6(4-) oxidation in 1 M KCl solution at the Pt nanoelectrode used as a tip in the scanning electrochemical microscope...
March 13, 2017: Journal of Physical Chemistry Letters
https://www.readbyqxmd.com/read/28284847/a-survey-on-some-biochemical-and-pharmacological-activities-of-venom-from-two-colombian-colubrid-snakes-erythrolamprus-bizona-double-banded-coral-snake-mimic-and-pseudoboa-neuwiedii-neuwied-s-false-boa
#17
Kristian A Torres-Bonilla, Rafael S Floriano, Raphael Schezaro-Ramos, Léa Rodrigues-Simioni, Maria Alice da Cruz-Höfling
Colombian colubrid snake venoms have been poorly studied. They represent a great resource of biological, ecological, toxinological and pharmacological research. We assessed some enzymatic properties and neuromuscular effects of Erythrolamprus bizona and Pseudoboa neuwiedii venoms from Colombia. Proteolytic, amidolytic and phospholipase A2 (PLA2) activities were analyzed using colorimetric assays and the neuromuscular activity was analyzed in chick biventer cervicis (BC) preparations. The venom of both species showed very low PLA2 and amidolytic activities; however, both exhibited high proteolytic activity, which in E...
March 9, 2017: Toxicon: Official Journal of the International Society on Toxinology
https://www.readbyqxmd.com/read/28284123/the-impact-of-high-hydrostatic-pressure-on-the-functionality-and-consumer-acceptability-of-reduced-sodium-naturally-cured-wieners
#18
Z Pietrasik, N J Gaudette, S P Johnston
The effects of high pressure processing (HPP; 600MPa for 3min at 8°C) on the quality and shelf life of reduced sodium naturally-cured wieners was studied. HPP did not negatively impact processing characteristics and assisted in extending shelf life of all wiener treatments up to a 12week storage period. At week 8, HPP wieners received higher acceptability scores, indicating HPP can effectively extend the sensory quality of products, including sodium reduced formulations containing natural forms of nitrite...
February 24, 2017: Meat Science
https://www.readbyqxmd.com/read/28274925/salt-supplementation-ameliorates-developmental-kidney-defects-in-cox-2-mice
#19
Patrick Slattery, Stefanie Frölich, Itamar Goren, Rolf Michael Nüsing
Deficiency of cyclooxygenase-2 (COX-2) activity in the early postnatal time course causes impairment of kidney development leading to kidney insufficiency. We hypothesize that impaired NaCl reabsorption during the first days of life is a substantial cause for nephrogenic defects observed in COX-2-/- mice and that salt supplementation corrects these defects. Daily injections of 0.8 mg/g/d NaCl for the first 10 days after birth ameliorated impaired kidney development in COX-2-/- pups resulting in an increase in glomerular size and reduction of immature superficial glomeruli...
March 8, 2017: American Journal of Physiology. Renal Physiology
https://www.readbyqxmd.com/read/28274112/partitioning-behavior-of-bisphenol-alternatives-bps-and-bpaf-compared-to-bpa
#20
Youn Jeong Choi, Linda S Lee
With the pressure to ban or limit the use of bisphenol A (BPA), production of alternatives such as bisphenol AF (BPAF) and bisphenol S (BPS) are increasing, but little is known on their partitioning behavior for use in assessing distribution in the ecosystem. Octanol-water (DowpH) and soil-water partitioning were measured at several pH values for BPA, BPAF, and BPS. Sorption isotherms were constructed from measured aqueous and soil phase concentrations and were fit sufficiently well with a linear sorption model...
March 9, 2017: Environmental Science & Technology
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