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https://www.readbyqxmd.com/read/28539820/bifidobacterium-pseudocatenulatum-cect-7765-supplementation-restores-altered-vascular-function-in-an-experimental-model-of-obese-mice
#1
María D Mauricio, Eva Serna, María Leonor Fernández-Murga, Jesica Portero, Martín Aldasoro, Soraya L Valles, Yolanda Sanz, José M Vila
Aims.Bifidobacterium pseudocatenulatum CECT 7765 improves metabolic and immunological altered functions in high fat fed mice, however little is known about the effects of potential probiotics on vascular reactivity. The aim of the present study was to investigate the effects of a potential probiotic strain, Bifidobacterium pseudocatenulatum CECT 7765, on vascular response in obese mice. Methods. Aorta samples were obtained from mice, which were divided into three groups: a control group, receiving a standard diet; an obese group, receiving a high-fat diet; and an obese group receiving high-fat diet and a daily dose of B...
2017: International Journal of Medical Sciences
https://www.readbyqxmd.com/read/28537067/multifunctional-triple-decker-inverse-12-metallacrown-4-sandwiching-halides
#2
Ling-Yu Guo, Hai-Feng Su, Mohamedally Kurmoo, Xingpo Wang, Quan-Qin Zhao, Shui-Chao Lin, Chen-Ho Tung, Di Sun, Lan-Sun Zheng
The inclusion of halogen ions into inverse 12-metallacrown-4 (inv-[12-MCCu(I), pz-4]) array generates six triple-decker complexes of this family, {(MX)2[CuI4(pz)4]3}, (Hpz = 4-nitropyrazole, MX = NaCl, 1; NaBr, 2; NaI, 3; KCl, 4; KBr, 5 and KI, 6). Single-crystal X-ray crystallography of six members in this family reveals their similar structural feature that is four CuI ions are bonded by four pz to form a square metallomacrocycle comprising four metal centers and eight N atoms, thus giving an inv-[12-MCCu(I), pz-4] motif...
May 24, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28534679/laser-induced-photofragmentation-fluorescence-imaging-of-alkali-compounds-in-flames
#3
Tomas Leffler, Christian Brackmann, Marcus Aldén, Zhongshan Li
Laser-induced photofragmentation fluorescence has been investigated for the imaging of alkali compounds in premixed laminar methane-air flames. An ArF excimer laser, providing pulses of wavelength 193 nm, was used to photodissociate KCl, KOH, and NaCl molecules in the post-flame region and fluorescence from the excited atomic alkali fragment was detected. Fluorescence emission spectra showed distinct lines of the alkali atoms allowing for efficient background filtering. Temperature data from Rayleigh scattering measurements together with simulations of potassium chemistry presented in literature allowed for conclusions on the relative contributions of potassium species KOH and KCl to the detected signal...
June 2017: Applied Spectroscopy
https://www.readbyqxmd.com/read/28534100/dose-dependent-differential-effects-of-in-vivo-exposure-of-cadmium-on-myometrial-activity-in-rats-involvement-of-vdcc-and-ca-2-mimicking-pathways
#4
Vivek K Saroj, Udayraj P Nakade, Abhishek Sharma, Soumen Choudhury, Sunil W Hajare, Satish K Garg
Present study was undertaken to study the effect of 28-days exposure of female adult rats to cadmium (Cd) in drinking water @ 3, 10 and 30 parts per million (ppm) on myometrial responsiveness to different spasmogens and unravel the possible mechanism of alterations in myometrial activity. Cadmium and Ca(2+) levels in blood and uterus were measured by atomic absorption spectroscopy while isometric tension in myometrial strips was measured using data acquisition system-based physiograph. Dose-dependent increase in levels of cadmium was observed in both blood and uterus while calcium was increased only in the uterus as compared to those in control...
May 22, 2017: Biological Trace Element Research
https://www.readbyqxmd.com/read/28531853/methane-production-in-an-anaerobic-osmotic-membrane-bioreactor-using-forward-osmosis-effect-of-reverse-salt-flux
#5
Sheng Li, Youngjin Kim, Sherub Phuntsho, Laura Chekli, Ho Kyong Shon, TorOve Leiknes, Noreddine Ghaffour
This study investigated the impact of reverse salt flux (RSF) on microbe community and bio-methane production in a simulated fertilizer driven FO-AnMBR system using KCl, KNO3 and KH2PO4 as draw solutes. Results showed that KH2PO4 exhibited the lowest RSF in terms of molar concentration 19.1mM/(m(2).h), while for KCl and KNO3 it was 32.2 and 120.8mM/(m(2).h), respectively. Interestingly, bio-methane production displayed an opposite order with KH2PO4, followed by KCl and KNO3. Pyrosequencing results revealed the presence of different bacterial communities among the tested fertilizers...
May 10, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28527981/an-identification-of-the-soft-polyelectrolyte-gel-like-layer-on-silica-colloids-using-atomic-force-and-electron-microscopy
#6
Jiří Škvarla, Juraj Škvarla
A procedure is introduced for measuring the radius of spherical colloid particles from the curvature of upper parts of their central cross-sectional profiles obtained by atomic force microscopy (AFM). To minimize the possible compression and displacement of the spheres, AFM is operated in a mode rendering a constant ultralow pN force on the tip. The procedure allows us to evaluate the mean radius of nearly monodisperse submicrometer spheres of silica in their natively hydrated state in aqueous electrolyte solutions, irrespective of whether they are coagulated or not...
May 16, 2017: Ultramicroscopy
https://www.readbyqxmd.com/read/28526859/evaluation-of-arteriolar-smooth-muscle-cell-function-in-an-ex-vivo-microvascular-network-model
#7
Jessica M Motherwell, Mohammad S Azimi, Kristine Spicer, Natascha G Alves, Nicholas A Hodges, Jerome W Breslin, Prasad V G Katakam, Walter L Murfee
An emerging challenge in tissue engineering biomimetic models is recapitulating the physiological complexity associated with real tissues. Recently, our laboratory introduced the rat mesentery culture model as an ex vivo experimental platform for investigating the multi-cellular dynamics involved in angiogenesis within an intact microvascular network using time-lapse imaging. A critical question remains whether the vessels maintain their functionality. The objective of this study was to determine whether vascular smooth muscle cells in cultured microvascular networks maintain the ability to constrict...
May 19, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28515572/a-family-of-fe-n-c-oxygen-reduction-electrocatalysts-for-microbial-fuel-cell-mfc-application-relationships-between-surface-chemistry-and-performances
#8
Carlo Santoro, Alexey Serov, Rohan Gokhale, Santiago Rojas-Carbonell, Lydia Stariha, Jonathan Gordon, Kateryna Artyushkova, Plamen Atanassov
Different iron-based cathode catalysts have been studied for oxygen reduction reaction (ORR) in neutral media and then applied into microbial fuel cells (MFC). The catalysts have been synthesized using sacrificial support method (SSM) using eight different organic precursors named Niclosamide, Ricobendazole, Guanosine, Succinylsulfathiazole, Sulfacetamide, Quinine, Sulfadiazine and Pyrazinamide. Linear Sweep Voltammetry (LSV) curves were obtained for the catalysts using a O2 saturated in 0.1 M potassium phosphate buffer and 0...
May 15, 2017: Applied Catalysis. B, Environmental
https://www.readbyqxmd.com/read/28514167/quantum-dot-peptide-fullerene-bioconjugates-for-visualization-of-in-vitro-and-in-vivo-cellular-membrane-potential
#9
Okhil Kumar Nag, Michael H Stewart, Jeffrey R Deschamps, Kimihiro Susumu, Eunkeu Oh, Vassiliy Tsytsarev, Qinggong Tang, Alexander L Efros, Roman Vaxenburg, Bryan J Black, Yungchia Chen, Thomas J O'Shaughnessy, Stella H North, Lauren D Field, Philip E Dawson, Joseph J Pancrazio, Igor L Medintz, Yu Chen, Reha S Erzurumlu, Alan L Huston, James B Delehanty
We report the development of a quantum dot (QD)-peptide-fullerene (C60) electron transfer (ET)-based nanobioconjugate for the visualization of membrane potential in living cells. The bioconjugate is comprised of (1) a central QD electron donor, (2) a membrane-inserting peptidyl linker, and (3) a C60 electron acceptor. The photoexcited QD donor engages in ET with the C60 acceptor resulting in quenching of QD photoluminescence (PL) that tracks positively with the number of C60 moieties arrayed around the QD. The nature of the QD-capping ligand also modulates the quenching efficiency; a neutral ligand coating facilitates greater QD quenching than a negatively charged carboxylated ligand...
May 17, 2017: ACS Nano
https://www.readbyqxmd.com/read/28513236/enhanced-gap-junctional-channel-activity-between-vascular-smooth-muscle-cells-in-cerebral-artery-of-spontaneously-hypertensive-rats
#10
Li-Jie Wang, Ke-Tao Ma, Wen-Yan Shi, Ying-Zi Wang, Lei Zhao, Xin-Yan Chen, Xin-Zhi Li, Xue-Wei Jiang, Zhong-Shuang Zhang, Li Li, Jun-Qiang Si
The aim of the present study is to investigate the effects of hypertension on the gap junctions between vascular smooth muscle cells (VSMCs) in the cerebral arteries (CAs) of spontaneously hypertensive rats (SHRs). The functions of gap junctions in the CAs of VSMCs in SHRs and control normotensive Wistar-Kyoto (WKY) rats were studied using whole-cell patch clamp recordings and pressure myography, and the expression levels of connexins were analyzed using reverse transcription-quantitative polymerase chain reaction and Western blot analyses...
May 17, 2017: Clinical and Experimental Hypertension: CHE
https://www.readbyqxmd.com/read/28512759/effect-of-ph-on-rheotaxis-of-bull-sperm-using-microfluidics
#11
T M El-Sherry, M A Abdel-Ghani, N S Abou-Khalil, M Elsayed, M Abdelgawad
The aim of the present research is to study the effect of pH values on the sperm rheotaxis properties. Semen collected from bulls was diluted with SOF medium (1:10). pH of the medium was adjusted using a digital pH meter to the following pH values: 6.0, 6.2, 6.4, 6.4, 6.8, 7.0. All kinetic parameters of sperm (n = 3,385) were determined through a computer-assisted sperm analysis (CASA) system using microfluidic devices with controlled flow velocity. The following parameters were determined: total motility (TM%), positive rheotaxis (PR%), straightline velocity (VSL, μm/s), average path velocity (VAP, μm/s), linearity (LIN, as VSL/VCL, %), beat cross-frequency (BCF, Hz) and curvilinear velocity (VCL, μm/s)...
May 16, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28509876/optimization-of-eps-production-and-characterization-by-a-halophilic-bacterium-kocuria-rosea-zjuqh-from-chaka-salt-lake-with-response-surface-methodology
#12
Di Gu, Yingchun Jiao, Jianan Wu, Zhengjie Liu, Qihe Chen
With the rising awareness of microbial exopolysaccharides (EPSs) application in various fields, halophilic microorganisms which produce EPSs have received broad attention. A newly identified Kocuria rosea ZJUQH CCTCC M2016754 was determined to be a moderate halobacterium on account of its successful adaption to the environment containing 10% NaCl. The optimal combination of fermentation medium compositions on EPS production was studied. In this work, a fractional factorial design was adopted to investigate the significant factors that affected EPS production...
May 16, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28507513/appetitive-olfactory-learning-and-long-term-associative-memory-in-caenorhabditis-elegans
#13
Saori Nishijima, Ichiro N Maruyama
Because of the relative simplicity of its nervous system, Caenorhabditis elegans is a useful model organism to study learning and memory at cellular and molecular levels. For appetitive conditioning in C. elegans, food has exclusively been used as an unconditioned stimulus (US). It may be difficult to analyze neuronal circuits for associative memory since food is a multimodal combination of olfactory, gustatory, and mechanical stimuli. Here, we report classical appetitive conditioning and associative memory in C...
2017: Frontiers in Behavioral Neuroscience
https://www.readbyqxmd.com/read/28504250/curcumin-enhances-vascular-contractility-via-induction-of-myocardin-in-mouse-smooth-muscle-cells
#14
Shao-Wei Sun, Wen-Juan Tong, Zi-Fen Guo, Qin-Hui Tuo, Xiao-Yong Lei, Cai-Ping Zhang, Duan-Fang Liao, Jian-Xiong Chen
A variety of cardiovascular diseases is accompanied by the loss of vascular contractility. This study sought to investigate the effects of curcumin, a natural polyphenolic compound present in turmeric, on mouse vascular contractility and the underlying mechanisms. After mice were administered curcumin (100 mg·kg-1·d-1, ig) for 6 weeks, the contractile responses of the thoracic aorta to KCl and phenylephrine were significantly enhanced compared with the control group. Furthermore, the contractility of vascular smooth muscle (SM) was significantly enhanced after incubation in curcumin (25 μmol/L) for 4 days, which was accompanied by upregulated expression of SM marker contractile proteins SM22α and SM α-actin...
May 1, 2017: Acta Pharmacologica Sinica
https://www.readbyqxmd.com/read/28504158/influence-of-osmotic-and-weight-pressure-on-water-release-from-polysaccharide-ionic-gels
#15
Komla Ako
Kappa-carrageenan (kC) is a polyelectrolyte biopolymer that forms gels if the kC concentration, the salt and the temperature are adequately chosen. Actually, under certain conditions, kC gels release water in a process called syneresis. In this contribution, the syneresis of gels containing 2g/L of kC has been studied as a function of potassium chloride concentration [KCl]. The syneresis decreases if the [KCl] is increased in the biopolymer solution (sol) before the gelation but increases if the [KCl] is increased after gelation by immersing the gels in KCl solution...
August 1, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28501417/prevalence-of-frailty-assessed-by-fried-and-kihon-checklist-indexes-in-a-prospective-cohort-study-design-and-demographics-of-the-kyoto-kameoka-longitudinal-study
#16
Yosuke Yamada, Hinako Nanri, Yuya Watanabe, Tsukasa Yoshida, Keiichi Yokoyama, Aya Itoi, Heiwa Date, Miwa Yamaguchi, Motoko Miyake, Emi Yamagata, Hajime Tamiya, Miho Nishimura, Mami Fujibayashi, Naoyuki Ebine, Mitsuyoshi Yoshida, Takeshi Kikutani, Eiichi Yoshimura, Kazuko Ishikawa-Takata, Minoru Yamada, Tomoki Nakaya, Yasuko Yoshinaka, Yoshinori Fujiwara, Hidenori Arai, Misaka Kimura
OBJECTIVE: The Kyoto-Kameoka Study was launched in 2011-2012 to identify the associations among food intake, nutritional status, physical activity, oral function, quality of life or social capital, the use of long-term care insurance (LTCI) system, and healthy lifespan in community-dwelling older people as a part of the World Health Organization Safe Community program. DESIGN: A prospective cohort study, reporting baseline demographics (cross-sectional data). SETTING AND PARTICIPANTS: We conducted 2 mailed self-administered questionnaire surveys; one is a complete population survey with a comprehensive survey of needs in the sphere of daily life (NSDL) that included 2 different frailty indexes, the Kihon Checklist (KCL) and the Fried phenotype, socioeconomic status, general and psychological health, and social relationships; followed by the more detailed Health and Nutrition Survey...
May 11, 2017: Journal of the American Medical Directors Association
https://www.readbyqxmd.com/read/28500670/coupled-effects-of-salt-and-pressure-on-catalytic-ability-of-rhizopus-chinensis-lipase
#17
Gang Chen, Lu Wang, Ming Miao, Chengsheng Jia, Biao Feng
BACKGROUND: Both the high pressure and environmental factors could influence the catalytic abilities of enzymes. This work investigated coupled effects of pressure and salts on Rhizopus chinensis lipase (RCL) to provide significative information for its further applications. RESULTS: The maximum activity of RCL was observed under 200 MPa at 40 °C. The highest activity was achieved at concentrations of 0.06 ~ 0.1 mol L(-1) for tested salts. The effect of monovalent cations on RCL activity followed the Hofmeister series (K(+)  > Na(+)  > Li(+) ) at 0...
May 13, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/28500449/concentration-polarization-phenomenon-in-the-case-of-mechanical-pressure-difference-on-the-membrane
#18
Sławomir Grzegorczyn, Andrzej Ślęzak, Brygida Przywara-Chowaniec
We analyzed the transport of KCl solutions through the bacterial cellulose membrane and concentration boundary layers (CBLs) near membrane with pressure differences on the membrane. The membrane was located in horizontal-plane between two chambers with different KCL solutions. The membrane was located in horizontal-plane between two chambers with different KCL solutions. As results from the elaborated model, gradient of KCL concentration in CBLs is maximal at membrane surfaces in the case when pressure difference on the membrane equals zero...
May 12, 2017: Journal of Biological Physics
https://www.readbyqxmd.com/read/28499248/influence-of-fertilisation-with-sewage-sludge-derived-preparation-on-selected-soil-properties-and-prairie-cordgrass-yield
#19
Ewa Ociepa, Maciej Mrowiec, Joanna Lach
The aim of the study was to evaluate the effect of using a fertilizer obtained from waste substances on selected physical and chemical properties of soil and biomass yield Spartina pectinate. The fertilizer used for soil (C) fertilisation contained sewage sludge (SS), waste soil fractions of brown coal (BC), brown coal ash (BCA) enriched with mineral potassium (K) fertilizer (C+SS+BC+BCA+K). The composition of the preparation was developed by the authors and adjusted to the quality of the fertilised soil and the individual characteristics of the plant...
May 9, 2017: Environmental Research
https://www.readbyqxmd.com/read/28497981/express-measurement-of-cerium-and-gadolinium-in-solid-licl-kcl-salt-via-laser-induced-breakdown-spectroscopy-libs
#20
Ammon N Williams, Keith C Bryce, Supathorn Phongikaroon
No abstract text is available yet for this article.
January 1, 2017: Applied Spectroscopy
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