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https://www.readbyqxmd.com/read/28434061/the-sources-of-calcium-for-noradrenaline-induced-contraction-in-the-human-thoracic-internal-artery
#1
Leszek Buzun, Beata Modzelewska, Anna Kostrzewska, Ewa Kleszczewska, Tomasz Kleszczewski
The aim of the present study was to examine the contribution of intracellular and extracellular calcium sources in contraction caused by noradrenaline (NA) of the human internal thoracic artery (ITA) in vitro. Distal segments of ITA were obtained from 20 patients (aged 38-73, at the time of routine coronary artery surgical revascularization (CABG)). Contractile responses to 10(-6) mol/L NA in the physiological salt solution and in Ca(2+)-free solution without and after incubation with 10(-6) mol/L thapsigargin (TSG) were recorded under isometric conditions...
April 22, 2017: Pflügers Archiv: European Journal of Physiology
https://www.readbyqxmd.com/read/28433399/simultaneous-68-ga-dotatate-positron-emission-tomography-magnetic-resonance-imaging-in-meningioma-target-contouring-feasibility-and-impact-upon-interobserver-variability-versus-positron-emission-tomography-computed-tomography-and-computed-tomography-magnetic
#2
J Maclean, N Fersht, K Sullivan, I Kayani, J Bomanji, J Dickson, C O'Meara, S Short
AIMS: The increasing use of highly conformal radiation techniques to treat meningioma confers a greater need for accurate targeting. Several groups have shown that positron emission tomography/computed tomography (PET/CT) information alters meningioma targets contoured by single observers, but whether this translates into improved accuracy has not been defined. As magnetic resonance imaging (MRI) is the cornerstone of meningioma target contouring, simultaneous PET/MRI may be superior to PET/CT...
April 19, 2017: Clinical Oncology: a Journal of the Royal College of Radiologists
https://www.readbyqxmd.com/read/28432808/mechanism-of-the-vasorelaxant-effect-induced-by-trans-4-methyl-%C3%AE-nitrostyrene-a-synthetic-nitroderivative-in-rat-thoracic-aorta
#3
Taylena Maria Teófilo, Loeste Arruda-Barbosa, Jussara Mathyelle Rodrigues-Silva, Joyce Karen Lima Vale, Rosivaldo Dos Santos Borges, Gloria Pinto Duarte, Pedro Jorge Caldas Magalhães, Saad Lahlou
Mechanisms underlying the vasorelaxant effects of trans-4-methyl-β-nitrostyrene (T4MeN) were studied in rat aortic rings. In endothelium-intact preparations, T4MeN fully and similarly relaxed contractions induced by phenylephrine (PHE) (IC50 = 61.41 [35.40-87.42] μM) and KCl (IC50 = 83.50 [56.63-110.50] μM). The vasorelaxant effect of T4MeN was unchanged by endothelium removal, pretreatment with L-NAME, indomethacin, tetraethylammonium, ODQ or MDL-12,330A. Under Ca(2+) -free conditions, T4MeN significantly reduced with a similar potency: (i) phasic contractions induced by PHE, but not by caffeine; (ii) contractions due to CaCl2 in aortic preparations stimulated with PHE (in the presence of verapamil) or high KCl; (iii) contractions evoked by the restoration of external Ca(2+) levels after depletion of intracellular Ca(2+) stores in the presence of thapsigargin...
April 22, 2017: Clinical and Experimental Pharmacology & Physiology
https://www.readbyqxmd.com/read/28432475/a-shinella-%C3%AE-n-acetylglucosaminidase-of-glycoside-hydrolase-family-20-displays-novel-biochemical-and-molecular-characteristics
#4
Junpei Zhou, Zhifeng Song, Rui Zhang, Caihong Chen, Qian Wu, Junjun Li, Xianghua Tang, Bo Xu, Junmei Ding, Nanyu Han, Zunxi Huang
β-N-Acetylglucosaminidases (GlcNAcases) are important for many biological functions and industrial applications. In this study, a glycoside hydrolase family 20 GlcNAcase from Shinella sp. JB10 was expressed in Escherichia coli BL21 (DE3). Compared to many GlcNAcases, the purified recombinant enzyme (rJB10Nag) exhibited a higher specificity activity (538.8 µmol min(-1) mg(-1)) or V max (1030.0 ± 82.1 µmol min(-1) mg(-1)) toward p-nitrophenyl β-N-acetylglucosaminide and N,N'-diacetylchitobiose (specificity activity of 35...
April 21, 2017: Extremophiles: Life Under Extreme Conditions
https://www.readbyqxmd.com/read/28430615/a-novel-mechanism-of-angiotensin-ii-regulated-placental-vascular-tone-in-the-development-of-hypertension-in-preeclampsia
#5
Qinqin Gao, Jiaqi Tang, Na Li, Xiuwen Zhou, Yongmei Li, Yanping Liu, Jue Wu, Yuxian Yang, Ruixiu Shi, Axin He, Xiang Li, Yingying Zhang, Jie Chen, Lubo Zhang, Miao Sun, Zhice Xu
The present study tested the hypothesis that angiotensin II plays a role in the regulation of placental vascular tone, which contributes to hypertension in preeclampsia. Functional and molecular assays were performed in large and micro placental and non-placental vessels from humans and animals. In human placental vessels, angiotensin II induced vasoconstrictions in 78.7% vessels in 155 tests, as referenced to KCl-induced contractions. In contrast, phenylephrine only produced contractions in 3.0% of 133 tests...
February 16, 2017: Oncotarget
https://www.readbyqxmd.com/read/28429218/modification-of-calcium-activated-chloride-currents-in-cerebellar-purkinje-neurons
#6
E A Vikhareva, V L Zamoyski, V V Grigoriev
The whole-cell voltage clamp technique was employed to record the total ionic currents in rat cerebellar Purkinje neurons. When intrapipette solution contained 120 mM KCl, replacement of the standard external physiological saline with Na-free solution resulted in appearance of inward tail current after the end of the depolarizing pulse. When intrapipette potassium ions were replaced for cesium ones, the tail currents were observed even in the presence of normal Na(+) concentration (140 mM) in the external solution...
April 21, 2017: Bulletin of Experimental Biology and Medicine
https://www.readbyqxmd.com/read/28429021/preparation-and-characterization-of-metastable-trigonal-layered-msb2o6-phases-m-co-ni-cu-zn-and-mg-and-considerations-on-fesb2o6
#7
A Yu Nikulin, E A Zvereva, V B Nalbandyan, I L Shukaev, A I Kurbakov, M D Kuchugura, G V Raganyan, Yu V Popov, V D Ivanchenko, A N Vasiliev
MSb2O6 compounds (M = Mg, Co, Ni, Cu, Zn) are known in the tetragonal trirutile forms, slightly distorted monoclinically with M = Cu due to the Jahn-Teller effect. In this study, using a low-temperature exchange reaction between ilmenite-type NaSbO3 and molten MSO4-KCl (or MgCl2-KCl) mixtures, these five compositions were prepared for the first time as trigonal layered rosiaite (PbSb2O6)-type phases. Upon heating, they irreversibly transform to the known phases via amorphous intermediates, in contrast to previously studied isostructural MnSb2O6, where the stable phase is structurally related to the metastable phase...
April 21, 2017: Dalton Transactions: An International Journal of Inorganic Chemistry
https://www.readbyqxmd.com/read/28428070/no-production-and-potassium-channels-activation-induced-by-crotalus-durissus-cascavella-underlie-mesenteric-artery-relaxation
#8
S S Santos, R L C Jesus, L O Simões, W P Vasconcelos, I A Medeiros, R C Veras, L L Casais-E-Silva, D F Silva
Animal toxins are natural resources for pharmacological studies. The venom of Crotalus durissus cascavella (C.d. cascavella) may be a source in the bio-prospecting of new anti-hypertensive agents. The aim of this study was to investigate vascular effects of the venom of C.d. cascavella in normotensive rats. Studies were performed using isolated mesenteric artery segments and aortic endothelial cells. The cumulative administration of the venom of C.d. cascavella (0.001-30 μg/mL) on phenylephrine (Phe; 10 μM) pre-contracted rings induced a concentration-dependent vasorelaxation in the presence of vascular endothelium (Emax = 47...
April 17, 2017: Toxicon: Official Journal of the International Society on Toxinology
https://www.readbyqxmd.com/read/28424671/functional-characterization-and-conditional-regulation-of-the-type-vi-secretion-system-in-vibrio-fluvialis
#9
Yuanming Huang, Pengcheng Du, Meng Zhao, Wei Liu, Yu Du, Baowei Diao, Jie Li, Biao Kan, Weili Liang
Vibrio fluvialis is an emerging foodborne pathogen of increasing public health concern. The mechanism(s) that contribute to the bacterial survival and disease are still poorly understood. In other bacterial species, type VI secretion systems (T6SSs) are known to contribute to bacterial pathogenicity by exerting toxic effects on host cells or competing bacterial species. In this study, we characterized the genetic organization and prevalence of two T6SS gene clusters (VflT6SS1 and VflT6SS2) in V. fluvialis. VflT6SS2 harbors three "orphan" hcp-vgrG modules and was more prevalent than VflT6SS1 in our isolates...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28420519/simultaneous-cul-de-sac-and-tubal-pregnancy-following-in%C3%A2-vitro-fertilization
#10
Cheng-En Hsieh, Yuh-Ming Hwu, Sheng-Hsiang Li, Robert Kuo-Kuang Lee
OBJECTIVE: The aim of this study is to share a valuable experience of heterotopic pregnancy following in vitro fertilization. CASE REPORT: A 37-year-old, gravida 3, para 2 (cesarean section 2 times), woman underwent in vitro fertilization with three embryos transferred. On Day 23 after the embryo transfer, right tubal pregnancy with a 0.7-cm gestational sac was found by ultrasound, and her serum β-human chorionic gonadotropin level was 81,388 mIU/mL. She underwent a laparotomy with right salpingectomy...
April 2017: Taiwanese Journal of Obstetrics & Gynecology
https://www.readbyqxmd.com/read/28420122/the-effect-of-salt-intake-and-potassium-supplementation-on-serum-gastrin-levels-in-chinese-adults-a-randomized-trial
#11
Yuan-Yuan Wang, Wen-Wen He, Yan-Chun Liu, Yi-Feng Lin, Lu-Fei Hong
Excess dietary salt is strongly correlated with cardiovascular disease, morbidity, and mortality. Conversely, potassium likely elicits favorable effects against cardiovascular disorders. Gastrin, which is produced by the G-cells of the stomach and duodenum, can increase renal sodium excretion and regulate blood pressure by acting on the cholecystokinin B receptor. The aim of our study was to assess the effects of altered salt and potassium supplementation on serum gastrin levels in humans. A total of 44 subjects (38-65 years old) were selected from a rural community in northern China...
April 14, 2017: Nutrients
https://www.readbyqxmd.com/read/28419939/the-5-ht4-receptor-agonist-prucalopride-does-not-facilitate-cholinergic-neurotransmission-in-circular-and-longitudinal-smooth-muscle-preparations-of-equine-mid-jejunum
#12
Romain Adelin Lefebvre, Chana Callens, Inge Van Colen, Catherine John Ghislaine Delesalle
BACKGROUND: Postoperative ileus (POI) remains an important cause of death in horses. The recently developed selective 5-HT4 receptor agonists such as prucalopride target 5-HT4 receptors on myenteric cholinergic neurons to enhance acetylcholine release and GI motility. No clearcut in vitro evaluation whether highly selective 5-HT4 receptor agonists enhance submaximal cholinergic neurotransmission towards the muscle layer has been performed in horses. OBJECTIVES: To identify functional 5-HT4 receptors in equine jejunum...
April 8, 2017: Research in Veterinary Science
https://www.readbyqxmd.com/read/28418247/high-and-stable-ionic-conductivity-in-2d-nanofluidic-ion-channels-between-boron-nitride-layers
#13
Si Qin, Dan Liu, Guang Wang, David Portehault, Christopher J Garvey, Yury Gogotsi, Weiwei Lei, Ying Chen
Achieving a high rate of ionic transport through porous membranes and ionic channels is important in numerous applications ranging from energy storage to water desalination, but still remains a challenge. Herein we show that ions can quickly pass through interlayer spaces in hydrated boron nitride (BN) membranes. Measurements of surface-charge governed ionic currents through BN conduits in a variety of salt solutions (KCl, NaCl and CaCl2) at low salt concentrations (<10-4 M) showed several orders of magnitude higher ionic conductivity compared to the bulk solution...
April 18, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/28416926/the-role-of-cyclooxygenase-enzymes-in-the-effects-of-losartan-and-lisinopril-on-the-contractions-of-rat-thoracic-aorta
#14
Fikriye Yasemin Ozatik, Bilgin Kaygisiz, Kevser Erol
OBJECTIVE: It was suggested that prostaglandins which are synthesized by cyclooxygenase (COX) enzymes contribute to the actions of angiotensin-converting enzyme (ACE) inhibition and angiotensin AT1 receptor antagonism and there is an interaction between ACE signaling pathway and COX enzymes. We aim to investigate the role of COX enzymes in the effects of losartan, an angiotensin II (Ang II) receptor antagonist or lisinopril, an ACE inhibitor, on the contractions of rat thoracic aorta in isolated tissue bath...
February 2017: Eurasian Journal of Medicine
https://www.readbyqxmd.com/read/28411813/voltammetric-determination-of-trace-amounts-of-diacetyl-at-a-mercury-meniscus-modified-silver-solid-amalgam-electrode-following-gas-diffusion-microextraction
#15
Rui M Ramos, Luís M Gonçalves, Vlastimil Vyskočil, José A Rodrigues
A new approach was developed for the determination of trace amounts of diacetyl in food products using gas-diffusion microextraction (GDME) and subsequent detection by differential pulse voltammetry (DPV) at a mercury meniscus modified silver solid amalgam electrode (m-AgSAE). Diacetyl is a vicinal diketone responsible for the buttery aroma in many fermented foods and beverages. Its determination is important not only for evaluation of the final product quality (note of mention: health related concerns were associated with continuous diacetyl exposure) but also to monitor fermentation...
July 1, 2017: Talanta
https://www.readbyqxmd.com/read/28407435/sl15-a-seminal-plasma-derived-lectin-from-the-sperm-of-llama-lama-glama
#16
Renato Zampini, Sabrina Sequeira, Martin Eduardo Argañaraz, Silvana Andrea Apichela
The oviductal sperm reservoir of South American camelids is formed when sperm bind to N-acetylgalactosamine (GalNAc) on the surface of oviductal epithelium. The aim of this study was to characterize the GalNAc-binding proteins on llama sperm, and to establish their origin. Sperm-adsorbed proteins were extracted with 0.5 M KCl in Hepes-balanced salts. Sperm-adsorbed and seminal plasma proteins were then subjected to ligand blotting for their GalNAc affinity, and the labeled bands were identified by mass spectrometry...
April 13, 2017: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/28407038/emmaw-computing-minimal-absent-words-in-external-memory
#17
Alice Heliou, Solon P Pissis, Simon J Puglisi
Motivation: The biological significance of minimal absent words has been investigated in genomes of organisms from all domains of life. For instance, three minimal absent words of the human genome were found in Ebola virus genomes (Silva et al., Bioinf., 2015). There exists an O(n)-time and O(n)-space algorithm for computing all minimal absent words of a sequence of length n on a fixed-sized alphabet based on suffix arrays (Barton et al., BMC Bioinf., 2014). A standard implementation of this algorithm, when applied to a large sequence of length n, requires more than 20n bytes of RAM...
April 12, 2017: Bioinformatics
https://www.readbyqxmd.com/read/28402412/effects-of-neutral-salts-and-ph-on-the-activity-and-stability-of-human-rnase-h2
#18
Misato Baba, Kenji Kojima, Rihoko Nakase, Shota Imai, Tomomi Yamasaki, Teisuke Takita, Robert J Crouch, Kiyoshi Yasukawa
Ribonuclease H (RNase H) specifically degrades the RNA of RNA/DNA hybrid. Recent study has shown that a single ribonucleotide is embedded in DNA double strand at every few thousand base pairs in human genome, and human RNase H2 is involved in its removal. Here, we examined the effects of neutral salts and pH on the activity and stability of human RNase H2. NaCl, KCl, RbCl, and NaBr increased the activity to 170-390% at 10-60 mM, while LiCl, LiBr, and CsCl inhibited it, suggesting that species of cation, but not anion, is responsible for the effect on activity...
April 11, 2017: Journal of Biochemistry
https://www.readbyqxmd.com/read/28399455/dioxins-degradation-and-reformation-during-mechanochemical-treatment
#19
Zhiliang Chen, Qiongjing Mao, Shengyong Lu, Alfons Buekens, Shuaixi Xu, Xu Wang, Jianhua Yan
Mechanochemical dechlorination and destruction of polychlorinated dibenzo-p-dioxins and -furans (PCDD/F) on fly ash from Municipal Solid Waste Incineration was tested with and without additives (CaO and CaO/aluminium powder). The first results disappointed because of obvious PCDD/F-reformation and a second test series was conducted after removing soluble salts (NaCl, KCl …) by thorough two-stage water washing. This second test series was successful and demonstrated good destruction results, especially with combined CaO/aluminium powder as additive...
April 4, 2017: Chemosphere
https://www.readbyqxmd.com/read/28398555/biophysical-and-functional-characterization-of-hippocalcin-mutants-responsible-for-human-dystonia
#20
Nordine Helassa, Svetlana V Antonyuk, Lu-Yun Lian, Lee P Haynes, Robert D Burgoyne
Dystonia is a neurological movement disorder that forces the body into twisting, repetitive movements or sometimes painful abnormal postures. With the advent of next-generation sequencing technologies, the homozygous mutations T71N and A190T in the neuronal calcium sensor (NCS) hippocalcin were identified as the genetic cause of primary isolated dystonia (DYT2 dystonia). However, the effect of these mutations on the physiological role of hippocalcin has not yet been elucidated. Using a multidisciplinary approach, we demonstrated that hippocalcin oligomerises in a calcium-dependent manner and binds to voltage-gated calcium channels...
April 7, 2017: Human Molecular Genetics
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