Yang C Zeng, Olivia J Young, Christopher M Wintersinger, Frances M Anastassacos, James I MacDonald, Giorgia Isinelli, Maxence O Dellacherie, Miguel Sobral, Haiqing Bai, Amanda R Graveline, Andyna Vernet, Melinda Sanchez, Kathleen Mulligan, Youngjin Choi, Thomas C Ferrante, Derin B Keskin, Geoffrey G Fell, Donna Neuberg, Catherine J Wu, David J Mooney, Ick Chan Kwon, Ju Hee Ryu, William M Shih
Multivalent presentation of ligands often enhances receptor activation and downstream signalling. DNA origami offers a precise nanoscale spacing of ligands, a potentially useful feature for therapeutic nanoparticles. Here we use a square-block DNA origami platform to explore the importance of the spacing of CpG oligonucleotides. CpG engages Toll-like receptors and therefore acts to activate dendritic cells. Through in vitro cell culture studies and in vivo tumour treatment models, we demonstrate that square blocks induce Th1 immune polarization when CpG is spaced at 3...
March 15, 2024: Nature Nanotechnology