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https://www.readbyqxmd.com/read/29237841/mammalian-adaptation-of-an-avian-influenza-a-virus-involves-stepwise-changes-in-ns1
#1
C Chauché, A Nogales, H Zhu, D Goldfarb, A I Ahmad Shanizza, Q Gu, C R Parrish, L Martínez-Sobrido, J F Marshall, P R Murcia
Influenza A viruses (IAVs) are common pathogens of birds that occasionally establish endemic infections in mammals. The processes and mechanisms that result in IAV mammalian adaptation are poorly understood. The viral non-structural 1 (NS1) protein counteracts the interferon (IFN) response, a central component of the host-species barrier.We characterised the NS1 proteins of equine influenza virus (EIV), a mammalian IAV lineage of avian origin. We showed that evolutionary distinct NS1s counteract the IFN response using different and mutually exclusive mechanisms: while the NS1s of early EIVs block general gene expression by binding to the cellular polyadenylation specific factor 30 (CPSF30), NS1s from more evolved EIVs specifically block the induction of IFN-stimulated genes by interfering with the JAK/STAT pathway...
December 13, 2017: Journal of Virology
https://www.readbyqxmd.com/read/29237829/unexpected-functional-divergence-of-bat-influenza-virus-ns1-proteins
#2
Hannah L Turkington, Mindaugas Juozapaitis, Nikos Tsolakos, Eugenia Corrales-Aguilar, Martin Schwemmle, Benjamin G Hale
Recently, two influenza A virus (FLUAV) genomes were identified in Central and South American bats. These sequences exhibit notable divergence from classical FLUAV counterparts, and functionally, bat FLUAV glycoproteins lack canonical receptor binding and destroying activity. Nevertheless, other features that distinguish these viruses from classical FLUAVs have yet to be explored. Here, we studied the viral non-structural protein, NS1, a virulence factor that modulates host signaling to promote efficient propagation...
December 13, 2017: Journal of Virology
https://www.readbyqxmd.com/read/29236357/live-attenuated-influenza-virus-vaccine-reduces-virus-shedding-of-newborn-piglets-in-the-presence-of-maternal-antibody
#3
Marika Genzow, Christa Goodell, Troy J Kaiser, Wesley Johnson, Marc Eichmeyer
BACKGROUND: Influenza A virus in swine (IAV-S) causes an acute respiratory disease of swine which results in great economic losses in pig production. Major control strategies include the use of killed vaccines (KV) in breeding females to confer passive immunity to their offspring. A bivalent H1N1 and H3N2 NS1-truncated live attenuated IAV-S vaccine has recently become available, which showed promising results in young pigs. OBJECTIVE: The aim of this study was to investigate the effect of an intranasal vaccination of newborn pigs with or without maternally derived antibodies (MDA) on virus shedding (via nasal swabs tested by virus isolation)...
December 13, 2017: Influenza and Other Respiratory Viruses
https://www.readbyqxmd.com/read/29226082/the-novel-mitochondria-localization-of-influenza-a-virus-ns1-visualized-by-flash-labeling
#4
Chuan-Fu Tsai, Hsin-Yi Lin, Wei-Li Hsu, Ching-Hsiu Tsai
The nonstructural protein 1 (NS1) of the influenza A virus (IAV) is a multifunctional protein that counteracts host cell antiviral responses and inhibits host cell pre-mRNA processing. NS1 contains two nuclear localization signals that facilitate NS1 shuttling between cytoplasm and nucleus. In this study, we initially observed the novel mitochondria localization of NS1 in a subset of transfected cells. We then further monitored the localization dynamics of the NS1 protein in live cells infected with IAV expressing NS1 with insertion of a tetracysteine-tag...
December 2017: FEBS Open Bio
https://www.readbyqxmd.com/read/29219127/dengue-fever-in-a-municipality-of-west-bengal-india-2015-an-outbreak-investigation
#5
Falguni Debnath, Manickam Ponnaiah, Pralay Acharya
BACKGROUND: In November 2015, death due to fever and increased number of fever cases were reported from Baranagar Municipality of North 24 Parganas district of West Bengal. OBJECTIVES: The episode was investigated with the objective to (1) confirm the existence of an outbreak, (2) describe it in terms of time, place, and person, (3) determine the cause of outbreak, and (4) recommend control measures. METHODS: Monthly incidence of dengue from 2012 to 2014 was calculated and compared with 2015 to confirm the outbreak...
October 2017: Indian Journal of Public Health
https://www.readbyqxmd.com/read/29218438/two-novel-bocaparvovirus-species-identified-in-wild-himalayan-marmots
#6
Yuanyun Ao, Xiaoyue Li, Lili Li, Xiaolu Xie, Dong Jin, Jiemei Yu, Shan Lu, Zhaojun Duan
Bocaparvovirus (BOV) is a genetically diverse group of DNA viruses and a possible cause of respiratory, enteric, and neurological diseases in humans and animals. Here, two highly divergent BOVs (tentatively named as Himalayan marmot BOV, HMBOV1 and HMBOV2) were identified in the livers and feces of wild Himalayan marmots in China, by viral metagenomic analysis. Five of 300 liver samples from Himalayan marmots were positive for HMBOV1 and five of 99 fecal samples from these animals for HMBOV2. Their nearly complete genome sequences are 4,672 and 4,887 nucleotides long, respectively, with a standard genomic organization and containing protein-coding motifs typical for BOVs...
December 5, 2017: Science China. Life Sciences
https://www.readbyqxmd.com/read/29218433/s8orf2-protein-of-infectious-salmon-anaemia-virus-is-a-rna-silencing-suppressor-and-interacts-with-salmon-salar-mov10-ssmov10-of-the-host-rnai-machinery
#7
Vandana Thukral, Bhavna Varshney, Rimatulhana B Ramly, Sanket S Ponia, Sumona Karjee Mishra, Christel M Olsen, Akhil C Banerjea, Sunil K Mukherjee, Rana Zaidi, Espen Rimstad, Sunil K Lal
The infectious salmon anaemia virus (ISAV) is a piscine virus, a member of Orthomyxoviridae family. It encodes at least 10 proteins from eight negative-strand RNA segments. Since ISAV belongs to the same virus family as Influenza A virus, with similarities in protein functions, they may hence be characterised by analogy. Like NS1 protein of Influenza A virus, s8ORF2 of ISAV is implicated in interferon antagonism and RNA-binding functions. In this study, we investigated the role of s8ORF2 in RNAi suppression in a well-established Agrobacterium transient suppression assay in stably silenced transgenic Nicotiana xanthi...
December 7, 2017: Virus Genes
https://www.readbyqxmd.com/read/29212246/the-interaction-between-nolc1-and-iav-ns1-protein-promotes-host-cell-apoptosis-and-reduces-virus-replication
#8
Chunyu Zhu, Fangliang Zheng, Junfeng Zhu, Meichen Liu, Na Liu, Xue Li, Li Zhang, Zaidong Deng, Qi Zhao, Hongsheng Liu
NS1 of the influenza virus plays an important role in the infection ability of the influenza virus. Our previous research found that NS1 protein interacts with the NOLC1 protein of host cells, however, the function of the interaction is unknown. In the present study, the role of the interaction between the two proteins in infection was further studied. Several analyses, including the use of a pull-down assay, Co-IP, western blot analysis, overexpression, RNAi, flow cytometry, etc., were used to demonstrate that the NS1 protein of H3N2 influenza virus interacts with host protein NOLC1 and reduces the quantity of NOLC1...
November 7, 2017: Oncotarget
https://www.readbyqxmd.com/read/29209371/corrigendum-to-dengue-virus-ns1-protein-as-a-diagnostic-marker-commercially-available-elisa-and-comparison-to-qrt-pcr-and-serological-diagnostic-assays-currently-used-by-the-state-of-florida
#9
Jason H Ambrose, Shamala Devi Sekaran, Azliyati Azizan
[This corrects the article DOI: 10.1155/2017/8072491.].
2017: Journal of Tropical Medicine
https://www.readbyqxmd.com/read/29197806/immunofluorescence-based-biosensor-for-the-determination-of-dengue-virus-ns1-in-clinical-samples
#10
Nadiya T Darwish, Shamala D Sekaran, Yatimah Alias, Sook Mei Khor
The sharp increase in incidence of dengue infection has necessitated the development of methods for the rapid diagnosis of this deadly disease. Here we report the design and development of a reliable, sensitive, and specific optical immunosensor for the detection of the dengue nonstructural protein 1 (NS1) biomarker in clinical samples obtained during early stages of infection. The present optical NS1 immunosensor comprises a biosensing surface consisting of specific monoclonal NS1 antibody for immunofluorescence-based NS1 antigen determination using fluorescein isothiocyanate (FITC) conjugated to IgG antibody...
November 30, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/29182305/point-of-use-nanobiosensor-for-detection-of-dengue-virus-ns1-antigen-in-adult-aedes-aegypti-a-potential-tool-for-improved-dengue-surveillance
#11
Daniel Aaron Wasik, Ashok Mulchandani, Marylynn V Yates
Dengue virus (DENV) and its primary mosquito vectors Aedes spp., have spread to every continent except Antarctica, causing outbreaks and autochthonous transmission in previously disease-free regions. Recently, the spread of other arboviruses carried by invasive Aedes spp., such as Chikungunya and Zika, seem to be following similar trends as DENV and have renewed interest in monitoring and modelling arboviruses within mosquito vectors. Unfortunately, current commercially available detection methods are designed for the diagnosis of DENV in humans or are too expensive and complex for sustainable monitoring...
November 28, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/29181006/protective-effect-of-panax-notoginseng-root-water-extract-against-influenza-a-virus-infection-by-enhancing-antiviral-interferon-mediated-immune-responses-and-natural-killer-cell-activity
#12
Jang-Gi Choi, Young-Hee Jin, Heeeun Lee, Tae Woo Oh, Nam-Hui Yim, Won-Kyung Cho, Jin Yeul Ma
Influenza is an acute respiratory illness caused by the influenza A virus, which causes economic losses and social disruption mainly by increasing hospitalization and mortality rates among the elderly and people with chronic diseases. Influenza vaccines are the most effective means of preventing seasonal influenza, but can be completely ineffective if there is an antigenic mismatch between the seasonal vaccine virus and the virus circulating in the community. In addition, influenza viruses resistant to antiviral drugs are emerging worldwide...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/29176643/development-of-a-quantitative-ns1-capture-enzyme-linked-immunosorbent-assay-for-early-detection-of-yellow-fever-virus-infection
#13
Taissa Ricciardi-Jorge, Juliano Bordignon, Andrea Koishi, Camila Zanluca, Ana Luiza Mosimann, Claudia Nunes Duarte Dos Santos
Yellow fever is an arboviral disease that causes thousands of deaths every year in Africa and the Americas. However, few commercial diagnostic kits are available. Non-structural protein 1 (NS1) is an early marker of several flavivirus infections and is widely used to diagnose dengue virus (DENV) infection. Nonetheless, little is known about the dynamics of Yellow fever virus (YFV) NS1 expression and secretion, to encourage its use in diagnosis. To tackle this issue, we developed a quantitative NS1-capture ELISA specific for YFV using a monoclonal antibody and recombinant NS1 protein...
November 24, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29170830/genetic-analysis-of-three-porcine-bocaparvoviruses-and-identification-of-a-natural-recombinant-breakpoint-in-ns1
#14
Yu Zhou, Jia Xu, Shi-Kang Zhu, Qing-Feng Meng, Zhi-Xiong Lin, Ru Chen, Ai-Dong Qian
In this study, we obtained the whole genomes of three porcine bocaparvovirus (PBoV) strains (GD6, GD10, and GD23) by polymerase chain reaction. Sequence analysis showed that all three field strains belonged to PBoV group 3 (G3). The phylogenetic trees based on NS1, NP1, and VP1 differed to the extent that these PBoVs were potentially more closely related to bocaparvoviruses known to infect other animals than to other PBoVs. GD6, GD10, and GD23 all included the conserved sequences YLGPF and HDXXY, with known phospholipase A2 activity...
November 23, 2017: Archives of Virology
https://www.readbyqxmd.com/read/29169429/-preparation-and-application-of-polyclonal-antibody-against-non-structural-protein-1-ns1-of-duck-tembusu-virus
#15
Qi Zhou, Guanzhi Su, Xue Xing, Xiaoya Zhou, Yipeng Shi, Zhongwen Tang, Xiangjun Song, Hongmei Liu, Guijun Wang
Objective To express prokaryotically the non-structural protein 1 (NS1) of duck Tembusu virus (DTMUV) and prepare NS1-specific polyclonal antibodies. Methods The NS1 gene of DTMUV strain AH-F10 was amplified by PCR, followed by subcloning and expression in the prokaryotic vector pET-32a. The recombinant NS1 protein was successfully expressed in Escherichia coli BL21 (DE3), and purified with hydroxymethyl urea and renatured by gradient centrifugation. The BALB/c mice were immunized with the purified recombinant NS1 protein to prepare polyclonal antibodies against the NS1 protein...
October 2017: Xi Bao Yu Fen Zi Mian Yi Xue za Zhi, Chinese Journal of Cellular and Molecular Immunology
https://www.readbyqxmd.com/read/29167907/definitive-tests-for-dengue-fever-when-and-which-should-i-use
#16
Hui Bin Yvonne Chan, Choon How How, Chung Wai Mark Ng
Dengue is a mosquito-borne viral disease that has established itself globally in both endemic and epidemic transmission cycles. While diagnostic decision-making for dengue is often guided by clinical judgement, definitive laboratory tests, including rapid point-of-care tests, have many advantages in the primary care setting. These include providing epidemiological data and diagnostic clarity for atypical cases, as well as contributing to patient education and compliance. This article discussed the types of diagnostic methods for dengue, when to use them and the appropriateness of each test...
November 2017: Singapore Medical Journal
https://www.readbyqxmd.com/read/29166950/global-phylogenetic-analysis-of-contemporary-aleutian-mink-disease-viruses-amdvs
#17
P Ryt-Hansen, E E Hagberg, M Chriél, T Struve, A G Pedersen, L E Larsen, C K Hjulsager
BACKGROUND: Aleutian mink disease has major economic consequences on the mink farming industry worldwide, as it causes a disease that affects both the fur quality and the health and welfare of the mink. The virus causing this disease is a single-stranded DNA virus of the genus Amdoparvovirus belonging to the family of Parvoviridae. In Denmark, infection with AMDV has largely been restricted to a region in the northern part of the country since 2001, affecting only 5% of the total Danish mink farms...
November 22, 2017: Virology Journal
https://www.readbyqxmd.com/read/29158907/chikungunya-outbreak-in-delhi-india-2016-report-on-coinfection-status-and-comorbid-conditions-in-patients
#18
N Kaur, J Jain, A Kumar, M Narang, M K Zakaria, A Marcello, D Kumar, R Gaind, S Sunil
Chikungunya fever is a major public health issue in India affecting billions. After 2010, the infection was in a decline until in 2016, when a massive outbreak affected the country. In this report, we present serologic and molecular investigations of 600 patient samples for chikungunya and dengue viruses along with clinical and comorbidity features. We recruited 600 patients during this outbreak and evaluated them for chikungunya and dengue virus antibodies and virus RNA through IgM, NS1 antigen and quantitative real-time PCR (qPCR)...
November 2017: New Microbes and New Infections
https://www.readbyqxmd.com/read/29152905/the-gait-translational-control-system
#19
REVIEW
Abul Arif, Peng Yao, Fulvia Terenzi, Jie Jia, Partho Sarothi Ray, Paul L Fox
The interferon (IFN)-γ-activated inhibitor of translation (GAIT) system directs transcript-selective translational control of functionally related genes. In myeloid cells, IFN-γ induces formation of a multiprotein GAIT complex that binds structural GAIT elements in the 3'-untranslated regions (UTRs) of multiple inflammation-related mRNAs, including ceruloplasmin and VEGF-A, and represses their translation. The human GAIT complex is a heterotetramer containing glutamyl-prolyl tRNA synthetase (EPRS), NS1-associated protein 1 (NSAP1), ribosomal protein L13a (L13a), and glyceraldehyde-3-phosphate dehydrogenase (GAPDH)...
November 20, 2017: Wiley Interdisciplinary Reviews. RNA
https://www.readbyqxmd.com/read/29141913/multiplexed-biomarker-panels-discriminate-zika-and-dengue-virus-infection-in-humans
#20
Guang Song, Hee-Sool Rho, Jianbo Pan, Pedro Ramos, Ki-Jun Yoon, Freddy A Medina, Emily M Lee, Daniel J Eichinger, Guo-Li Ming, Jorge L Muñoz-Jordan, Hengli Tang, Ignacio Pino, Hongjun Song, Jiang Qian, Heng Zhu
Zika virus (ZIKV) and dengue virus (DENV) are closely related flaviviruses that cause widespread, acute febrile illnesses, notably microcephaly for fetuses of infected pregnant women. Detecting the viral cause of these illnesses is paramount to determine risks to patients, counsel pregnant women, and help fight outbreaks. A combined diagnostic algorithm for ZIKV and DENV requires Reverse transcription polymerase chain reaction (RT-PCR) and IgM antibody detection. RT-PCR differentiates between DENV and ZIKV infections during the acute phases of infection, but differentiation based on IgM antibodies is currently nearly impossible in endemic areas...
November 15, 2017: Molecular & Cellular Proteomics: MCP
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