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https://www.readbyqxmd.com/read/28100412/virome-of-us-bovine-calf-serum
#1
Mohammadreza Sadeghi, Beatrix Kapusinszky, Danielle M Yugo, Tung Gia Phan, Xutao Deng, Isis Kanevsky, Tanja Opriessnig, Amelia R Woolums, David J Hurley, Xiang-Jin Meng, Eric Delwart
Using viral metagenomics we analyzed four bovine serum pools assembled from 715 calves in the United States. Two parvoviruses, bovine parvovirus 2 (BPV2) and a previously uncharacterized parvovirus designated as bosavirus (BosaV), were detected in 3 and 4 pools respectively and their complete coding sequences generated. Based on NS1 protein identity, bosavirus qualifies as a member of a new species in the copiparvovirus genus. Also detected were low number of reads matching ungulate tetraparvovirus 2, bovine hepacivirus, and several papillomaviruses...
January 15, 2017: Biologicals: Journal of the International Association of Biological Standardization
https://www.readbyqxmd.com/read/28095684/effect-of-the-protein-corona-on-antibody-antigen-binding-in-nanoparticle-sandwich-immunoassays
#2
Helena de Puig, Irene Bosch, Marc Carré-Camps, Kimberly Hamad-Schifferli
We investigated the effect of the protein corona on the function of nanoparticle (NP) antibody (Ab) conjugates in dipstick sandwich immunoassays. Ab specific for Zika virus nonstructural protein 1 (NS1) were conjugated to gold NPs, and another anti-NS1 Ab was immobilized onto the nitrocellulose membrane. Sandwich immunoassay formation was influenced by whether the strip was run in corona forming conditions, i.e., in human serum. Strips run in buffer or pure solutions of bovine serum albumin exhibited false positives, but those run in human serum did not...
January 18, 2017: Bioconjugate Chemistry
https://www.readbyqxmd.com/read/28094237/a-multiplex-microsphere-immunoassay-for-zika-virus-diagnosis
#3
Susan J Wong, Andrea Furuya, Jing Zou, Xuping Xie, Alan P Dupuis, Laura D Kramer, Pei-Yong Shi
Rapid and accurate diagnosis of infectious agents is essential for patient care, disease control, and countermeasure development. The present serologic diagnosis of Zika virus (ZIKV) infection relies mainly on IgM-capture ELISA which is confounded with the flaw of cross-reactivity among different flaviviruses. In this communication, we report a multiplex microsphere immunoassay (MIA) that captures the diagnostic power of viral envelope protein (that elicits robust, yet cross-reactive antibodies to other flaviviruses) and the differential power of viral nonstructural proteins NS1 and NS5 (that induce more virus-type specific antibodies)...
January 10, 2017: EBioMedicine
https://www.readbyqxmd.com/read/28093537/dengue-epidemic-in-postconflict-swat-district-khyber-pakhtunkhwa-pakistan-2013
#4
Mamoona Chaudhry, Saeed Ahmad, Hamad Bin Rashid, Iftikhar Ud Din
Swat, a lush green valley of 1.3 million persons, remained under militant insurgency from 2007 to 2009, which damaged the health infrastructure. An outbreak of dengue fever (DF) was declared in the valley in 2013. To investigate this outbreak, we established active surveillance of national hospitals and private clinics, reviewed available clinical and laboratory records, and conducted entomological survey. From August to November 2013, 16,000 suspected patients with acute febrile illness were presented to health facilities...
January 16, 2017: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/28076660/influenza-a-virus-ns1-protein-induced-jnk-activation-and-apoptosis-are-not-functionally-linked
#5
Wolfgang Nacken, Viktor Wixler, Christina Ehrhardt, Stephan Ludwig
Expression of the influenza A virus (IAV) non-structural protein NS1 results in the activation of c-Jun N-terminal kinase (JNK). Both NS1 and JNK are involved in apoptosis induction. To investigate their interrelationship, we stably expressed a tamoxifen inducible NS1 oestrogen receptor fusion-protein (NS1ERT) in mammalian cells. Upon tamoxifen stimulation, NS1ERT expressing cells partially rescued the attenuated replication of NS1-deficient IAVs and also inhibited interferon up-regulation, confirming the functional competence of NS1ERT...
January 11, 2017: Cellular Microbiology
https://www.readbyqxmd.com/read/28062078/-coexistence-of-acute-appendicitis-and-dengue-fever-a-case-report
#6
Juan Fidel Osuna-Ramos, Carlos Silva-Gracia, Gerardo Joel Maya-Vacio, Alejandra Romero-Utrilla, Efrén Rafael Ríos-Burgueño, Jesús Salvador Velarde-Félix
BACKGROUND: Dengue is the most important human viral disease transmitted by mosquitoes. It can be asymptomatic or it can present in any of its 3clinical forms: Dengue fever, dengue haemorrhagic fever and dengue shock syndrome. However, some atypical manifestations have been reported in surgical emergencies caused by acute appendicitis in patients with dengue fever. CLINICAL CASE: We report the case of an 18-year-old Mexican male who presented to the emergency department of the General Hospital of Culiacan, Sinaloa, with symptoms of dengue fever, accompanied by crampy abdominal pain with positive Rovsing and Dunphy signs...
January 3, 2017: Cirugia y Cirujanos
https://www.readbyqxmd.com/read/28060934/a-cohort-study-of-adult-patients-with-severe-dengue-in-taiwanese-intensive-care-units-the-elderly-and-aptt-prolongation-matter-for-prognosis
#7
Chih-Cheng Hsieh, Cong-Tat Cia, Jen-Chieh Lee, Junne-Ming Sung, Nan-Yao Lee, Po-Lin Chen, Te-Hui Kuo, Jo-Yen Chao, Wen-Chien Ko
BACKGROUND: There was a large dengue outbreak in Taiwan in 2015, in which the ages of the affected individuals were higher than those in other countries. The aim of this study was to explore the characteristics and prognostic factors for adults with severe dengue in intensive care units (ICUs). METHODS: All adults admitted to ICUs with dengue virus infection (DENV) at a medical center from July 1, 2015 to December 31, 2015 were enrolled. DENV was diagnosed by the presence of serum NS1 antigen, IgM antibodies to dengue virus, or dengue virus RNA by real-time reverse transcriptase polymerase chain reaction...
January 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28060552/the-root-extract-of-gentiana-macrophylla-pall-alleviates-b19-ns1-exacerbated-liver-injuries-in-nzb-w-f1-mice
#8
Ming-Jen Sheu, Chun-Ching Chiu, Deng-Jye Yang, Tsai-Ching Hsu, Bor-Show Tzang
The nonstructural protein NS1 of human parvovirus B19 (B19) is known to exacerbate disease activity in systemic lupus erythematosus (SLE). However, no specific medicine for B19 infection is available. The roots of Gentiana macrophylla Pall. (GM), the traditional Chinese medicine "Qinjiao," have been used for centuries to treat rheumatic disease, including SLE. Herein, we aimed to investigate the effects of GM root extract (100 and 300 mg/kg body weight) on B19-NS1-exacerbated liver injury in NZB/W F1 mice; liver tissues were assessed by hematoxylin-eosin staining and immunoblotting...
January 2017: Journal of Medicinal Food
https://www.readbyqxmd.com/read/28056773/transcriptomic-analysis-of-human-norovirus-ns1-2-protein-highlights-a-multifunctional-role-in-murine-monocytes
#9
Zabeen Lateef, Gregory Gimenez, Estelle S Baker, Vernon K Ward
BACKGROUND: The GII.4 Sydney 2012 strain of human norovirus (HuNoV) is a pandemic strain that is responsible for the majority of norovirus outbreaks in healthcare settings. The function of the non-structural (NS)1-2 protein from HuNoV is unknown. RESULTS: In silico analysis of human norovirus NS1-2 protein showed that it shares features with the murine NS1-2 protein, including a disordered region, a transmembrane domain and H-box and NC sequence motifs. The proteins also contain caspase cleavage and phosphorylation sites, indicating that processing and phosphorylation may be a conserved feature of norovirus NS1-2 proteins...
January 5, 2017: BMC Genomics
https://www.readbyqxmd.com/read/28052239/honeysuckle-aqueous-extract-and-induced-let-7a-suppress-dengue-virus-type-2-replication-and-pathogenesis
#10
Ying-Ray Lee, Siao-Fen Yeh, Xiao-Ming Ruan, Hao Zhang, Sheng-Da Hsu, Hsien-Da Huang, Chang-Chi Hsieh, Yee-Shin Lin, Trai-Ming Yeh, Hsiao-Sheng Liu, Dai-Di Gan
ETHNOPHARMACOLOGICAL RELEVANCE: Honeysuckle (Lonicera japonica Thunb.), a traditional Chinese herb, has widely been used to treat pathogen infection. However, the underlying-mechanism remains elusive. AIMS OF THE STUDY: To reveal the host microRNA (miRNA) profile with the anti-viral activity after honeysuckle treatment. MATERIALS AND METHODS: Here we reveal the differentially expressed miRNAs by Solexa(®) deep sequencing from the blood of human and mice after the aqueous extract treatment...
January 1, 2017: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/28042949/recombinant-parvoviruses-armed-to-deliver-cxcl4l1-and-cxcl10-are-impaired-in-their-antiangiogenic-and-antitumoral-effects-in-a-kaposi-sarcoma-tumor-model-due-to-the-chemokines-interference-with-the-virus-cycle
#11
Christiane Dinsart, Kalliopi Pervolaraki, Alexandra Stroh-Dege, Muriel Lavie, Isabelle Ronsse, Jean Rommelaere, Jo Van Damme, Katrien Van Raemdonck, Sofie Struyf
Application of oncolytic viruses is a valuable option to broaden the armament of anti-cancer therapies as these combine specific cytotoxic effects and immune-stimulating properties. The self-replicating H-1 parvovirus (H-1PV) is a prototypical oncolytic virus that besides targeting tumor cells also infects endothelial cells, thus combining oncolytic and angiostatic traits. To increase its therapeutic value, H-1PV can be armed with cytokines or chemokines to enhance the immunological response. Some chemokines, more specifically the CXCR3 ligands CXCL4L1 and CXCL10 combine immune-stimulating properties with angiostatic activity...
January 2, 2017: Human Gene Therapy
https://www.readbyqxmd.com/read/28042883/utility-of-dengue-ns1-antigen-rapid-diagnostic-test-for-use-in-difficult-to-reach-areas-and-its-comparison-with-dengue-ns1-elisa-and-qrt-pcr
#12
Mohan K Shukla, Neeru Singh, Ravendra K Sharma, Pradip V Barde
The objective of this study was to demonstrate the utility of dengue virus (DENV) non structural protein 1 (NS1) based rapid diagnostic test (RDT) for use in tribal and difficult to reach areas for early dengue (DEN) diagnosis in acute phase patients and evaluate its sensitivity and specificity against DENV NS1 enzyme linked immune sorbent assay (ELISA) and real time reverse transcriptase polymerase chain reaction (qRT-PCR) The DENV NS1 RDT was used for preliminary diagnosis during outbreaks in difficult to reach rural and tribal areas...
January 2, 2017: Journal of Medical Virology
https://www.readbyqxmd.com/read/28040064/klf5-is-involved-in-regulation-of-ifitm1-2-and-3-genes-during-h5n1-virus-infection-in-a549-cells
#13
H-F Wang, L Chen, J Luo, H-X He
The interferon-induced transmembrane (IFITM) proteins usually protect cells from many virus infections by viral entry and replication. Thus, the present study aimed to identification novel regulation factors of IFITM genes. Our data showed up-regulation of IFITM1, IFITM2, and IFITM3 genes in A549 by H5N1 virus infection. However, IFITM1 was not affected, IFITM2 and IFITM3 increases expression following different concentration H5N1 virus infection in HEK293T cells. This is the first time to find differences of IFITM1 transcription in different cells with the H5N1 virus infection...
November 30, 2016: Cellular and Molecular Biology
https://www.readbyqxmd.com/read/28036247/intracellular-degradation-and-localization-of-ns1-of-tbev-affects-its-protective-properties
#14
Yulia Kuzmenko, Elizaveta Starodubova, Anastasia Shevtsova, Lubov Chernokhaeva, Anastasia Latanova, Olga Preobrazhenskaia, Andrey Timofeev, Galina Karganova, Vadim Karpov
Currently many DNA vaccines against infectious diseases are in clinical trials however their efficacy is needed to be improved. Potency of DNA immunogen can be optimized by targeting technologies. In a current study to increase the efficacy of NS1encoded by plasmid the proteasome targeting was applied. NS1 variants with or without translocation sequence and with signal of proteasomal degradation of ornithine decarboxylase were tested for expression, localization, protein turnover, proteasomal degradation and protection properties...
December 30, 2016: Journal of General Virology
https://www.readbyqxmd.com/read/28034883/an-evidence-based-algorithm-for-early-prognosis-of-severe-dengue-in-the-outpatient-setting
#15
Minh Tuan Nguyen, Thi Nhan Ho, Van Vinh Chau Nguyen, Thanh Hung Nguyen, Manh Tuan Ha, Van Tram Ta, Le Da Ha Nguyen, Loi Phan, Khoi Quang Han, Thi Hue Kien Duong, Nguyen Bich Chau Tran, Wills Bridget, Marcel Wolbers, Cameron P Simmons
BACKGROUND: Early prediction of severe dengue could significantly assist patient triage and case management METHODS: We prospectively investigated 7563 children with ≤3 days of fever recruited in the outpatient departments of six hospitals in southern Vietnam between 2010 and 2013. The primary endpoint of interest was severe dengue (2009 WHO Guidelines) and pre-defined risk variables were collected at the time of enrolment to enable prognostic model development. RESULTS: The analysis population comprised 7544 patients, of whom 2060 (27...
December 28, 2016: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
https://www.readbyqxmd.com/read/28031845/dengue-shock-syndrome
#16
Sreenivasa Rao Sudulagunta, Mahesh Babu Sodalagunta, Mona Sepehrar, Shiva Kumar Bangalore Raja, Aravinda Settikere Nataraju, Mounica Kumbhat, Deepak Sathyanarayana, Siddharth Gummadi, Hemanth Kumar Burra
Dengue fever is a mosquito-borne arthropod-borne viral (arboviral) tropical disease in humans affecting 50-528 million people worldwide. The acute abdominal complications of dengue fever are acute appendicitis, acute pancreatitis, acute acalculous cholecystitis and non-specific peritonitis. Acute pancreatitis with new onset diabetes in dengue shock syndrome (DSS) is very rarely reported. We describe a case of 30-year-old man admitted in intensive care unit and was diagnosed with DSS with RT-PCR, NS1 antigen and dengue IgM antibody being positive...
November 2016: Oxford Medical Case Reports
https://www.readbyqxmd.com/read/28027928/dengue-virus-non-structural-protein-1-expression-and-associated-risk-factors-among-febrile-patients-attending-university-of-abuja-teaching-hospital-nigeria
#17
Idris Abdullahi Nasir, Olubunmi Olajide Agbede, Amos Dangana, Marycelin Baba, Abubakar Shehu Haruna
BACKGROUND: Dengue is a mosquito-borne and neglected tropical viral disease that has been reported to be hyper-endemic in Nigeria. However, this is the first dengue study in Abuja. OBJECTIVE: This hospital-based cross-sectional study investigated the prevalence of Dengue virus (DENV) non-structural protein-1 (NS1) antigenaemia, anti-Dengue virus IgG and their associated risk factors among febrile patients attending the University of Abuja Teaching Hospital (UATH), Nigeria...
December 24, 2016: Virus Research
https://www.readbyqxmd.com/read/28018746/identifying-candidate-targets-of-immune-responses-in-zika-virus-based-on-homology-to-epitopes-in-other-flavivirus-species
#18
Xiaojun Xu, Kerrie Vaughan, Daniela Weiskopf, Alba Grifoni, Michael S Diamond, Alessandro Sette, Bjoern Peters
INTRODUCTION: The current outbreak of Zika virus has resulted in a massive effort to accelerate the development of ZIKV-specific diagnostics and vaccines. These efforts would benefit greatly from the definition of the specific epitope targets of immune responses in ZIKV, but given the relatively recent emergence of ZIKV as a pandemic threat, few such data are available. METHODS: We used a large body of epitope data for other Flaviviruses that was available from the IEDB for a comparative analysis against the ZIKV proteome in order to project targets of immune responses in ZIKV...
November 15, 2016: PLoS Currents
https://www.readbyqxmd.com/read/28012140/imported-dengue-in-two-tertiary-italian-hospitals-use-of-rapid-diagnostic-tests
#19
G Calleri, I Torta, F Gobbi, A Angheben, F Lipani, A Lucchini, E Burdino, V Ghisetti, P Caramello
Dengue fever is growing at a global level both as number of cases and as geographic area of endemicity. Italy is not in endemic area, but the competent vector Aedes albopictus is widespread in this country, so that the possibility of introduction of the infection cannot be ruled out. We retrospectively collected demographic, clinical, and laboratory data about consecutive cases diagnosed in Torino and Negrar-Verona in the period 2010-2015. One hundred thirteen cases of dengue were observed, with an increasing trend during years...
December 23, 2016: Bulletin de la Société de Pathologie Exotique
https://www.readbyqxmd.com/read/28006649/serodiagnosis-of-zika-virus-zikv-infections-by-a-novel-ns1-based-elisa-devoid-of-cross-reactivity-with-dengue-virus-antibodies-a-multicohort-study-of-assay-performance-2015-to-2016
#20
Katja Steinhagen, Christian Probst, Christiane Radzimski, Jonas Schmidt-Chanasit, Petra Emmerich, Marjan van Esbroeck, Janke Schinkel, Martin P Grobusch, Abraham Goorhuis, Jens M Warnecke, Erik Lattwein, Lars Komorowski, Andrea Deerberg, Sandra Saschenbrecker, Winfried Stöcker, Wolfgang Schlumberger
Serological diagnosis of Zika virus (ZIKV) infections is challenging due to high cross-reactivity between flaviviruses. We evaluated the diagnostic performance of a novel anti-ZIKV ELISA based on recombinant ZIKV non-structural protein 1 (NS1). Assay sensitivity was examined using sera from 27 patients with reverse transcription (RT)-PCR-confirmed and 85 with suspected ZIKV infection. Specificity was analysed using sera from 1,015 healthy individuals. Samples from 252 patients with dengue virus (n = 93), West Nile virus (n = 34), Japanese encephalitis virus (n = 25), chikungunya virus (n = 19) or Plasmodium spp...
December 15, 2016: Euro Surveillance: Bulletin Européen sur les Maladies Transmissibles, European Communicable Disease Bulletin
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