keyword
MENU ▼
Read by QxMD icon Read
search

NS1

keyword
https://www.readbyqxmd.com/read/29655694/tatanan-a-from-the-acorus-calamus-l-root-inhibited-dengue-virus-proliferation-and-infections
#1
Xingang Yao, Yun Ling, Songxin Guo, Wenyu Wu, Shijun He, Qing Zhang, Min Zou, Kutty Selva Nandakumar, Xiaoguang Chen, Shuwen Liu
BACKGROUND: Acorus calamus l. (Acoraceae) is a well-known traditional Chinese medicinal plant, whose root are historically mainly used to treat neurodegenerative diseases, and for cholera treatment. This datum strongly indicates the antimicrobial activity of A. calamus. PURPOSE: Our goal is to find the active constituents of A. calamus to treat dengue virus (DENV) infections, and to study the effects and mechanisms of these active substances. METHODS: The root of A...
March 15, 2018: Phytomedicine: International Journal of Phytotherapy and Phytopharmacology
https://www.readbyqxmd.com/read/29643248/a-single-amino-acid-at-the-polymerase-acidic-protein-determines-the-pathogenicity-of-influenza-b-viruses
#2
Joon-Yong Bae, Ilseob Lee, Jin Il Kim, Sehee Park, Kirim Yoo, Miso Park, Gayeong Kim, Mee Sook Park, Joo-Yeon Lee, Chun Kang, Kisoon Kim, Man-Seong Park
Influenza B virus (IBV) is one of the human respiratory viruses and the targets of seasonal vaccination. However, the bifurcation of two antigenically distinct lineages of IBVs makes it difficult to arrange proper medical countermeasures. Moreover, compared with pathogenicity-related molecular markers known for influenza A virus, little has been known for IBVs. To understand pathogenicity caused by IBVs, we investigated the molecular determinants of IBV pathogenicity in animal models. After serial lung-to-lung passages of Victoria lineage B/Brisbane/60/2008 (Vc_BR60) and Yamagata lineage B/Wisconsin/01/2010 (Ym_WI01) viruses in BALB/c mice, we identified the mouse-adapted Vc_BR60 (maVc_BR60) and Ym_WI01 (maYm_WI01) viruses, respectively...
April 11, 2018: Journal of Virology
https://www.readbyqxmd.com/read/29624615/efficacy-and-synergy-of-live-attenuated-and-inactivated-influenza-vaccines-in-young-chickens
#3
Hyesun Jang, Mohamed Elaish, Mahesh Kc, Michael C Abundo, Amir Ghorbani, John M Ngunjiri, Chang-Won Lee
Outbreaks of novel highly pathogenic avian influenza viruses have been reported in poultry species in the United States since 2014. These outbreaks have proven the limitations of biosecurity control programs, and new tools are needed to reinforce the current avian influenza control arsenal. Some enzootic countries have implemented inactivated influenza vaccine (IIV) in their control programs, but there are serious concerns that a long-term use of IIV without eradication may result in the selection of novel antigenically divergent strains...
2018: PloS One
https://www.readbyqxmd.com/read/29621534/diversity-of-dengue-virus-3-genotype-iii-in-jeddah-saudi-arabia
#4
Anwar M Hashem, Sayed S Sohrab, Sherif A El-Kafrawy, Adly M M Abd-Alla, Saeid Abo El-Ela, Turki S Abujamel, Ahmed M Hassan, Suha A Farraj, Noura A Othman, Remi N Charrel, Esam I Azhar
BACKGROUND: Dengue is the most important arboviral disease in tropical and subtropical countries. Dispersal of the vector and an increase in migratory flow between countries have led to large epidemics and severe clinical outcomes. Over the past 20 years, dengue epidemics have become more wide-spread and frequent. Previous studies have shown that dengue is endemic in Jeddah, Makkah and Al-Madinah in western Saudi Arabia as well as in Jazan region in the southern part of the country. The four serotypes of dengue virus (DENV) have been reported from western Saudi Arabia...
April 2, 2018: Acta Tropica
https://www.readbyqxmd.com/read/29616396/genomic-analysis-of-dengue-virus-serotype-1-denv-1-genotypes-from-surabaya-indonesia
#5
Benediktus Yohan, Puspa Wardhani, Hidayat Trimarsanto, A Aryati, R Tedjo Sasmono
Dengue has caused a significant public health impact globally. With the diverse genetic of the causative viruses, analysis of dengue virus (DENV) genomes is important to supplement epidemiological data with information that can be used to reconstruct the history of epidemics in time and space. We have reported the clinical and virological characteristics of dengue in Surabaya, Indonesia and revealed the presence of all four DENV serotypes and the predominance of DENV-1. The further classification of Surabaya DENV-1 into two different genotypes warrants in-depth genomic analysis to study the dynamics of both genotypes and their contribution to virus evolution, virus transmission, and disease...
April 3, 2018: Virus Genes
https://www.readbyqxmd.com/read/29608995/coat-protein-complex-i-facilitates-dengue-virus-production
#6
Nopprarat Tongmuang, Umpa Yasamut, Pucharee Songprakhon, Thanyaporn Dechtawewat, Shilu Malakar, Sansanee Noisakran, Pa-Thai Yenchitsomanus, Thawornchai Limjindaporn
Dengue hemorrhagic fever is a life-threatening disease caused by the dengue virus (DENV). After DENV enters into host cells, it replicates to generate viral particles to infect other cells. DENV exploits components of the cellular trafficking pathway to achieve effective virion production. Understanding of the proteins required for this trafficking process is essential for revealing the pathogenesis of DENV infection. Coat protein complex and soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs), two host protein families in the cellular trafficking pathway, were investigated to elucidate their respective roles during DENV infection...
March 30, 2018: Virus Research
https://www.readbyqxmd.com/read/29607366/blood-group-ab-is-associated-with-severe-forms-of-dengue-virus-infection
#7
Kalamathy Murugananthan, Sivaganesh Subramaniyam, Thirunavukarasu Kumanan, Leigh Owens, Natkunam Ketheesan, Faseeha Noordeen
Several viral and host factors are believed to contribute to the development of severe forms of dengue such as dengue haemorrhagic fever (DHF) and dengue shock syndrome (DSS) following a dengue virus (DENV) infection. The pathogenesis of DHF/DSS is not fully understood, however, host factors like ABO blood groups have been shown to contribute to the severity of DENV infection. The present study investigated the association of blood groups with severity of DENV infection in the northern region of Sri Lanka. The blood-groups of 405 patients positive for DENV NS1 antigen and anti-DENV IgM/IgG were determined using the standard haemagglutination assay recommended by the national blood bank/s...
March 2018: Virusdisease
https://www.readbyqxmd.com/read/29607357/simultaneous-detection-and-serotyping-of-dengue-infection-using-single-tube-multiplex-cdc-dengue-real-time-rt-pcr-from-india
#8
Shashi Sharma, Kundan Tandel, Surabhi Danwe, Puneet Bhatt, P K Dash, Praveer Ranjan, K R Rathi, Rajiv Mohan Gupta, M M Parida
Four antigenically different dengue virus serotypes (DENV-1, DENV-2, DENV-3 and DENV-4) are known to cause infections in humans. Some of these are known to cause more severe disease than the others. Chances for developing Dengue hemorrhagic fever-dengue shock syndrome (DHF-DSS) increases significantly with history of previous infection with one of the four serotypes. Therefore, early diagnosis, serotyping and providing early warning of dengue fever epidemics to concerned authorities becomes very important for better patient outcome and to curb the rapid spread in the community...
March 2018: Virusdisease
https://www.readbyqxmd.com/read/29593218/identification-and-characterization-of-a-novel-rodent-bocavirus-from-different-rodent-species-in-china
#9
Chi Zhang, Fenglin Song, Leshan Xiu, Yang Liu, Jian Yang, Lisi Yao, Junping Peng
Members in the genus Bocaparvovirus are closely related to human health and have a wide host range. The diverse hosts raise the possibility of crossing species barrier, which is a feature of emerging viruses. Among the mammalian hosts, rodents are generally acknowledged to be important reservoirs of emerging viruses. Here, rodent samples collected from six provinces and autonomous regions of China (Liaoning, Inner Mongolia, Tibet, Xinjiang, Guangxi and Yunnan) were used to investigate the prevalence and distribution of bocaparvoviruses...
March 29, 2018: Emerging Microbes & Infections
https://www.readbyqxmd.com/read/29590589/molecular-mechanisms-of-tight-binding-through-fuzzy-interactions
#10
Qingliang Shen, Jie Shi, Danyun Zeng, Baoyu Zhao, Pingwei Li, Wonmuk Hwang, Jae-Hyun Cho
Many intrinsically disordered proteins (IDPs) form fuzzy complexes upon binding to their targets. Although many IDPs are weakly bound in fuzzy complexes, some IDPs form high-affinity complexes. One example is the nonstructural protein 1 (NS1) of the 1918 Spanish influenza A virus, which hijacks cellular CRKII through the strong binding affinity (Kd ∼10 nM) of its proline-rich motif (PRMNS1 ) to the N-terminal Src-homology 3 domain of CRKII. However, its molecular mechanism remains elusive. Here, we examine the interplay between structural disorder of a bound PRMNS1 and its long-range electrostatic interactions...
March 27, 2018: Biophysical Journal
https://www.readbyqxmd.com/read/29588874/clinical-and-laboratory-profile-of-zika-and-dengue-infected-patients-lessons-learned-from-the-co-circulation-of-dengue-zika-and-chikungunya-in-brazil
#11
Elzinandes Leal Azeredo, Flavia Barreto Dos Santos, Luciana Santos Barbosa, Thiara Manuele Alves Souza, Jessica Badolato-Corrêa, Juan Camilo Sánchez-Arcila, Priscila Conrado Guerra Nunes, Luzia Maria de-Oliveira-Pinto, Ana Maria de Filippis, Márcia Dal Fabbro, Izilyanne Hoscher Romanholi, Rivaldo Venancio da Cunha
BACKGROUND: The current triple epidemic caused by dengue, zika and chikungunya constitutes a serious health problem in Brazil. The aim of this study was to investigate acute samples (up to the 7 days of symptoms) from patients presenting acute fever syndrome suspected as arboviral infection and characterize the clinical and laboratorial profile during the co-circulation of dengue, zika and chikungunya in Campo Grande, Mato Grosso do Sul (MS), midwest region of Brazil. METHODS: All suspected cases (n=134) were tested by using serological and molecular diagnostic tests including DENV, ZIKV and CHIKV RT-PCR, Dengue nonstructural protein 1 (NS1) antigen capture ELISA, anti- DENV IgM ELISA and anti-CHIKV IgM ELISA...
February 15, 2018: PLoS Currents
https://www.readbyqxmd.com/read/29587792/major-contribution-of-the-rna-binding-domain-of-ns1-in-the-pathogenicity-and-replication-potential-of-an-avian-h7n1-influenza-virus-in-chickens
#12
Sascha Trapp, Denis Soubieux, Alexandra Lidove, Evelyne Esnault, Adrien Lion, Vanaique Guillory, Alan Wacquiez, Emmanuel Kut, Pascale Quéré, Thibaut Larcher, Mireille Ledevin, Virginie Nadan, Christelle Camus-Bouclainville, Daniel Marc
BACKGROUND: Non-structural protein NS1 of influenza A viruses harbours several determinants of pathogenicity and host-range. However it is still unclear to what extent each of its two structured domains (i.e. RNA-binding domain, RBD, and effector domain, ED) contribute to its various activities. METHODS: To evaluate the respective contributions of the two domains, we genetically engineered two variants of an H7N1 low pathogenicity avian influenza virus harbouring amino-acid substitutions that impair the functionality of either domain...
March 27, 2018: Virology Journal
https://www.readbyqxmd.com/read/29587786/effects-of-the-s42-residue-of-the-h1n1-swine-influenza-virus-ns1-protein-on-interferon-responses-and-virus-replication
#13
Jinghua Cheng, Chunling Zhang, Jie Tao, Benqiang Li, Ying Shi, Huili Liu
BACKGROUND: The influenza A virus non-structural protein 1 (NS1) is a multifunctional protein that plays an important role in virus replication, virulence and inhibition of the host antiviral immune response. In the avian influenza virus or human influenza virus, specific amino acids of NS1 have been shown to be important for the virus to antagonize host antiviral defenses and promote viral replication. However, little research has been reported regarding the swine influenza virus (SIV) NS1 protein...
March 27, 2018: Virology Journal
https://www.readbyqxmd.com/read/29584637/mutations-in-the-non-structural-protein-coding-sequence-of-protoparvovirus-h-1pv-enhance-the-fitness-of-the-virus-and-show-key-benefits-regarding-the-transduction-efficiency-of-derived-vectors
#14
Hamidreza Hashemi, Alexandra-Larisa Condurat, Alexandra Stroh-Dege, Nadine Weiss, Carsten Geiss, Jill Pilet, Carles Cornet Bartolomé, Jean Rommelaere, Nathalie Salomé, Christiane Dinsart
Single nucleotide changes were introduced into the non-structural (NS) coding sequence of the H-1 parvovirus (PV) infectious molecular clone and the corresponding virus stocks produced, thereby generating H1-PM-I, H1-PM-II, H1-PM-III, and H1-DM. The effects of the mutations on viral fitness were analyzed. Because of the overlapping sequences of NS1 and NS2, the mutations affected either NS2 (H1-PM-II, -III) or both NS1 and NS2 proteins (H1-PM-I, H1-DM). Our results show key benefits of PM-I, PM-II, and DM mutations with regard to the fitness of the virus stocks produced...
March 27, 2018: Viruses
https://www.readbyqxmd.com/read/29574656/differences-in-type-i-interferon-response-in-human-lung-epithelial-cells-infected-by-highly-pathogenic-h5n1-and-low-pathogenic-h11n1-avian-influenza-viruses
#15
Milind M Thube, Pratip Shil, Rewati Kasbe, Avinash A Patil, Shailesh D Pawar, Jayati Mullick
Influenza A virus infection induces type I interferons (IFNs α/β) which activate host antiviral responses through a cascade of IFN signaling events. Herein, we compared highly pathogenic H5N1 and low pathogenic H11N1 avian influenza viruses isolated from India, for their replication kinetics and ability to induce IFN-β and interferon-stimulating genes (ISGs). The H5N1 virus showed a higher replication rate and induced less IFN-β and ISGs compared to the H11N1 virus when grown in the human lung epithelial A549 cells, reflecting the generation of differential innate immune responses during infection by these viruses...
March 24, 2018: Virus Genes
https://www.readbyqxmd.com/read/29570266/enhancing-antibody-serodiagnosis-using-a-controlled-peptide-co-immobilization-strategy
#16
Laura Sola, Paola Gagni, Ilda D'Annessa, Riccardo Capelli, Camilla Bertino, Alessandro Romanato, Francesco Damin, Greta Bergamaschi, Edoardo Marchisio, Angela Cuzzocrea, Mauro Bombaci, Renata Grifantini, Marcella Chiari, Giorgio Colombo, Alessandro Gori, Marina Cretich
Antigen immunoreactivity is often determined by surface regions defined by the 3D juxtapositions of amino acids stretches that are not continuous in the linear sequence. As such, mimicking an antigen immunoreactivity by means of putative linear peptide epitopes for diagnostic purposes is not trivial. Here we present a straightforward and robust method to extend the reach of immune-diagnostic probes design by co-presenting peptides belonging to the same antigenic surface. In this case study focused on a computationally predicted Zika virus NS1 protein putative antigenic region, we reached a diagnostic confidence by the oriented and spatially controlled co-immobilization of peptide sequences found adjacent within the protein fold, that cooperatively interacted to provide enhanced immunoreactivity with respect to single linear epitopes...
March 23, 2018: ACS Infectious Diseases
https://www.readbyqxmd.com/read/29567514/development-of-an-anti-dengue-ns1-igg-elisa-to-evaluate-exposure-to-dengue-virus
#17
Eduardo J M Nascimento, James K George, Melissa Velasco, Matthew I Bonaparte, Lingyi Zheng, Carlos DiazGranados, Ernesto T A Marques, James W Huleatt
Dengue virus infection elicits immune responses to multiple viral antigens including antibodies to dengue non-structural protein 1 (NS1) which are rapidly induced and detected within days of infection. The recombinant, live, attenuated, tetravalent dengue vaccine (CYD-TDV; Sanofi Pasteur) uses the yellow fever vaccine virus as a back-bone but expresses dengue virus pre-membrane and envelop proteins. Since CYD-TDV does not express dengue NS1, we evaluated the utility of dengue NS1-specific IgG antibodies as biomarkers of dengue exposure in CYD-TDV recipients and controls...
March 19, 2018: Journal of Virological Methods
https://www.readbyqxmd.com/read/29563291/a-naturally-occurring-deletion-in-the-effector-domain-of-h5n1-swine-influenza-virus-nonstructural-protein-1-regulates-viral-fitness-and-host-innate-immunity
#18
Junyong Wang, Yan Zeng, Shuai Xu, Jiayun Yang, Wanbing Wang, Bo Zhong, Jinying Ge, Lei Yin, Zhigao Bu, Hong-Bing Shu, Hualan Chen, Cao-Qi Lei, Qiyun Zhu
Nonstructural protein 1 (NS1) of influenza A virus regulates innate immune responses via various mechanisms. We previously showed that a naturally occurring deletion (the EALQR motif) in the NS1 effector domain of an H5N1 swine-origin avian influenza virus impairs the inhibition of type I interferon (IFN) in chicken fibroblasts and attenuates virulence in chickens. Here, we found that the virus bearing this deletion in its NS1 effector domain showed diminished inhibition of IFN-related cytokine expression and attenuated virulence in mice...
March 21, 2018: Journal of Virology
https://www.readbyqxmd.com/read/29558158/interactome-analysis-of-ns1-protein-encoded-by-influenza-a-h7n9-virus-reveals-an-inhibitory-role-of-ns1-in-host-mrna-maturation
#19
Rei-Lin Kuo, Chi-Jene Chen, Ee-Hong Tam, Chung-Guei Huang, Li-Hsin Li, Zong-Hua Li, Pei-Chia Su, Hao-Ping Liu, Chih-Ching Wu
Influenza A virus infections can result in severe respiratory diseases. The H7N9 subtype of avian influenza A virus has been transmitted to humans and caused severe disease and death. Nonstructural protein 1 (NS1) of influenza A virus is a virulence determinant during viral infection. To elucidate the functions of the NS1 encoded by influenza A H7N9 virus (H7N9 NS1), interaction partners of H7N9 NS1 in human cells were identified with immunoprecipitation followed by SDS-PAGE coupled with liquid chromatography-tandem mass spectrometry (GeLC-MS/MS)...
March 20, 2018: Journal of Proteome Research
https://www.readbyqxmd.com/read/29557328/dengue-fever-as-an-emerging-infection-in-southeast-iran
#20
Mostafa Heydari, Maliheh Metanat, Mohammad-Ali Rouzbeh-Far, Seyed Mehdi Tabatabaei, Mohammad Rakhshani, Nahid Sepehri-Rad, Maryam Keshtkar-Jahromi
Dengue fever (DF) is a mosquito-borne acute viral disease presenting with hemorrhagic manifestations in severe cases. Southeast Iran is in close proximity to Pakistan, an endemic country for DF. This cross-sectional study was conducted in the Sistan and Baluchestan province in the southeast of Iran to investigate possibility of DF (immunoglobulin M [IgM], immunoglobulin G [IgG], and nonstructural protein 1 [NS1] antigen tests) in 60 clinically suspected patients (April 2013 to August 2015). NS1 protein was detected in 5% ( N = 3), at least one of the antibodies (IgM and/ or IgG) was detected in 11% ( N = 7) of the samples...
March 19, 2018: American Journal of Tropical Medicine and Hygiene
keyword
keyword
8539
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"