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https://www.readbyqxmd.com/read/28323816/an-ns-segment-exonic-splicing-enhancer-regulates-influenza-a-virus-replication-in-mammalian-cells
#1
Xiaofeng Huang, Min Zheng, Pui Wang, Bobo Wing-Yee Mok, Siwen Liu, Siu-Ying Lau, Pin Chen, Yen-Chin Liu, Honglian Liu, Yixin Chen, Wenjun Song, Kwok-Yung Yuen, Honglin Chen
Influenza virus utilizes host splicing machinery to process viral mRNAs expressed from both M and NS segments. Through genetic analysis and functional characterization, we here show that the NS segment of H7N9 virus contains a unique G540A substitution, located within a previously undefined exonic splicing enhancer (ESE) motif present in the NEP mRNA of influenza A viruses. G540A supports virus replication in mammalian cells while retaining replication ability in avian cells. Host splicing regulator, SF2, interacts with this ESE to regulate splicing of NEP/NS1 mRNA and G540A substitution affects SF2-ESE interaction...
March 21, 2017: Nature Communications
https://www.readbyqxmd.com/read/28316881/occurrence-of-concurrent-infections-with-multiple-serotypes-of-dengue-viruses-during-2013-2015-in-northern-kerala-india
#2
Manchala Nageswar Reddy, Ranjeet Dungdung, Lathika Valliyott, Rajendra Pilankatta
BACKGROUND: Dengue is a global human public health threat, causing severe morbidity and mortality. The occurrence of sequential infection by more than one serotype of dengue virus (DENV) is a major contributing factor for the induction of Dengue Hemorrhagic Fever (DHF) and Dengue Shock Syndrome (DSS), two major medical conditions caused by DENV infection. However, there is no specific drug or vaccine available against dengue infection. There are reports indicating the increased incidence of concurrent infection of dengue in several tropical and subtropical regions...
2017: PeerJ
https://www.readbyqxmd.com/read/28295400/paper-based-point-of-care-testing-for-cost-effective-diagnosis-of-acute-flavivirus-infections
#3
F Bedin, L Boulet, E Voilin, G Theillet, A Rubens, C Rozand
Flavivirus infections are a serious healthcare concern in tropical and subtropical countries. Although well-established laboratory tests can provide early diagnosis of acute dengue or Zika infections, access to these tests is limited in developing countries, presenting an urgent need to develop simple, rapid, and robust diagnostic tools. Microfluidic Paper-based Analytical Devices (µPAD), are typically rapid, cost-effective, user-friendly, and they can be used as diagnostic tools for the diagnosis of these infections at Point of Care settings...
March 11, 2017: Journal of Medical Virology
https://www.readbyqxmd.com/read/28293273/mathematical-modelling-of-immune-parameters-in-the-evolution-of-severe-dengue
#4
M K Premaratne, S S N Perera, G N Malavige, Saroj Jayasinghe
Aims. Predicting the risk of severity at an early stage in an individual patient will be invaluable in preventing morbidity and mortality caused by dengue. We hypothesized that such predictions are possible by analyzing multiple parameters using mathematical modeling. Methodology. Data from 11 adult patients with dengue fever (DF) and 25 patients with dengue hemorrhagic fever (DHF) were analyzed. Multivariate statistical analysis was performed to study the characteristics and interactions of parameters using dengue NS1 antigen levels, dengue IgG antibody levels, platelet counts, and lymphocyte counts...
2017: Computational and Mathematical Methods in Medicine
https://www.readbyqxmd.com/read/28284209/increased-sensitivity-of-ns1-elisa-by-heat-dissociation-in-acute-dengue-4-cases
#5
Sibelle Nogueira Buonora, Flavia Barreto Dos Santos, Regina Paiva Daumas, Sonia Regina Lambert Passos, Manoela Heringer da Silva, Monique Rocha de Lima, Rita Maria Ribeiro Nogueira
BACKGROUND: Dengue is an acute febrile illness considered the major arboviral disease in terms of morbidity, mortality, economic impact and dissemination worldwide. Brazil accounts for the highest notification rate, with circulation of all four dengue serotypes. The NS1 antigen is a dengue highly conserved specific soluble glycoprotein essential for viral replication and viability that can be detected 0 to 18 days from the onset of fever (peak first 3 days). It induces a strong humoral response and is known as a complement-fixing antigen...
March 11, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28281773/b-cell-epitopes-of-african-horse-sickness-virus-serotype-4-recognised-by-immune-horse-sera
#6
Evans M Mathebula, Frederika E Faber, Wouter Van Wyngaardt, Antoinette Van Schalkwyk, Alri Pretorius, Jeanni Fehrsen
Identifying antigenic proteins and mapping their epitopes is important for the development of diagnostic reagents and recombinant vaccines. B-cell epitopes of African horse sickness virus (AHSV) have previously been mapped on VP2, VP5, VP7 and NS1, using mouse, rabbit and chicken monoclonal antibodies. A comprehensive study of the humoral immune response of five vaccinated horses to AHSV-4 antigenic peptides was undertaken. A fragmented-genome phage display library expressing a repertoire of AHSV-4 peptides spanning the entire genome was constructed...
February 24, 2017: Onderstepoort Journal of Veterinary Research
https://www.readbyqxmd.com/read/28273991/acute-renal-failure-in-dengue-infection
#7
Girish Pamappa Vakrani, Nambakam Tanuja Subramanyam
INTRODUCTION: Acute Renal Failure (RF) is a rare but well recognized complication of Dengue Infection (DI). There has been paucity of published data regarding renal involvement in DI. AIM: The aim of the present study was to elucidate different clinical presentations, disease outcomes of DI. To study the frequency, severity and predictors of RF in DI. MATERIALS AND METHODS: Patients diagnosed either as Dengue Fever (DF) or Dengue Haemorrhagic Fever/Dengue Shock Syndrome (DHF/DSS) respectively were enrolled for this study...
January 2017: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/28269669/pca-criterion-for-svm-mlp-classifier-for-flavivirus-biomarker-from-salivary-sers-spectra-at-febrile-stage
#8
A R M Radzol, Khuan Y Lee, W Mansor, I S Omar
Non-structural protein (NS1) has been conceded as one of the biomarkers for flavivirus that causes diseases with life threatening consequences. NS1 is an antigen that allows detection of the illness at febrile stage, mostly from blood samples currently. Our work here intends to define an optimum model for PCA-SVM with MLP kernel for classification of flavivirus biomarker, NS1 molecule, from SERS spectra of saliva, which to the best of our knowledge has never been explored. Since performance of the model depends on the PCA criterion and MLP parameters, both are examined in tandem...
August 2016: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/28267794/the-interdependencies-of-viral-load-the-innate-immune-response-and-clinical-outcome-in-children-presenting-to-the-emergency-department-with-respiratory-syncytial-virus-associated-bronchiolitis
#9
Felipe-Andrés Piedra, Minghua Mei, Vasanthi Avadhanula, Reena Mehta, Letisha Aideyan, Roberto P Garofalo, Pedro A Piedra
Respiratory syncytial virus (RSV) causes significant infant morbidity and mortality. For decades severe RSV-induced disease was thought to result from an uncontrolled host response to viral replication, but recent work suggests that a strong innate immune response early in infection is protective. To shed light on host-virus interactions and the viral determinants of disease, copy numbers of five RSV genes (NS1, NS2, N, G, F) were measured by quantitative real-time polymerase chain reaction (qPCR) in nasal wash samples from children with RSV-associated bronchiolitis...
2017: PloS One
https://www.readbyqxmd.com/read/28264028/parvovirus-b19-ns1-protein-induces-cell-cycle-arrest-at-g2-phase-by-activating-the-atr-cdc25c-cdk1-pathway
#10
Peng Xu, Zhe Zhou, Min Xiong, Wei Zou, Xuefeng Deng, Safder S Ganaie, Steve Kleiboeker, Jianxin Peng, Kaiyu Liu, Shengqi Wang, Shui Qing Ye, Jianming Qiu
Human parvovirus B19 (B19V) infection of primary human erythroid progenitor cells (EPCs) arrests infected cells at both late S-phase and G2-phase, which contain 4N DNA. B19V infection induces a DNA damage response (DDR) that facilitates viral DNA replication but is dispensable for cell cycle arrest at G2-phase; however, a putative C-terminal transactivation domain (TAD2) within NS1 is responsible for G2-phase arrest. To fully understand the mechanism underlying B19V NS1-induced G2-phase arrest, we established two doxycycline-inducible B19V-permissive UT7/Epo-S1 cell lines that express NS1 or NS1mTAD2, and examined the function of the TAD2 domain during G2-phase arrest...
March 2017: PLoS Pathogens
https://www.readbyqxmd.com/read/28263312/diagnosis-of-zika-virus-infection-on-a-nanotechnology-platform
#11
Bo Zhang, Benjamin A Pinsky, Jeyarama S Ananta, Su Zhao, Shylaja Arulkumar, Hao Wan, Malaya K Sahoo, Janaki Abeynayake, Jesse J Waggoner, Clay Hopes, Meijie Tang, Hongjie Dai
We developed a multiplexed assay on a plasmonic-gold platform for measuring IgG and IgA antibodies and IgG avidity against both Zika virus (ZIKV) and dengue virus (DENV) infections. In contrast to IgM cross-reactivity, IgG and IgA antibodies against ZIKV nonstructural protein 1 (NS1) antigen were specific to ZIKV infection, and IgG avidity revealed recent ZIKV infection and past DENV-2 infection in patients in dengue-endemic regions. This assay could enable specific diagnosis of ZIKV infection over other flaviviral infections...
March 6, 2017: Nature Medicine
https://www.readbyqxmd.com/read/28261244/continuous-spectrum-leds-promote-seedling-quality-traits-and-performance-of-quercus-ithaburensis-var-macrolepis
#12
Sonia Smirnakou, Theoharis Ouzounis, Kalliopi M Radoglou
Regulation of the growth, development, and quality of plants by the control of light quality has attracted extensive attention worldwide. The aim of this study was to examine the effects of continuous LED spectrum for indoor plant pre-cultivation and to investigate the morphological and physiological responses of a common broadleaved tree species in Mediterranean environment, Quercus ithaburensis var. macrolepis at seedling developmental stage. Thus, the seedlings were pre-cultivated for 28 days, under five different LED light qualities: (1) Fluorescent (FL) as control light (2) L20AP67 (high in green and moderate in far-red), (3) AP673L (high in green and red), (4) G2 (highest in red and far-red), AP67 (high in blue, red, and far-red), and (5) NS1 (highest in blue and green and lowest in far-red) LEDs...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28261172/the-complement-system-in-flavivirus-infections
#13
Jonas N Conde, Emiliana M Silva, Angela S Barbosa, Ronaldo Mohana-Borges
The incidence of flavivirus infections has increased dramatically in recent decades in tropical and sub-tropical climates worldwide, affecting hundreds of millions of people each year. The Flaviviridae family includes dengue, West Nile, Zika, Japanese encephalitis, and yellow fever viruses that are typically transmitted by mosquitoes or ticks, and cause a wide range of symptoms, such as fever, shock, meningitis, paralysis, birth defects, and death. The flavivirus genome is composed of a single positive-sense RNA molecule encoding a single viral polyprotein...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28254680/enhancing-the-sensitivity-of-dengue-virus-serotype-detection-by-rt-pcr-among-infected-children-in-india
#14
Syed Fazil Ahamed, Rosario Vivek, Shalini Kotabagi, Kaustuv Nayak, Anmol Chandele, Murali-Krishna Kaja, Anita Shet
Dengue surveillance relies on reverse transcription-polymerase chain reaction (RT-PCR), for confirmation of dengue virus (DENV) serotypes. We compared efficacies of published and modified primer sets targeting envelope (Env) and capsid-premembrane (C-prM) genes for detection of circulating DENV serotypes in southern India. Acute samples from children with clinically-diagnosed dengue were used for RT-PCR testing. All samples were also subjected to dengue serology (NS1 antigen and anti-dengue-IgM/IgG rapid immunochromatographic assay)...
February 28, 2017: Journal of Virological Methods
https://www.readbyqxmd.com/read/28250123/influenza-a-virus-virulence-depends-on-two-amino-acids-in-the-n-terminal-domain-of-its-ns1-protein-facilitating-inhibition-of-pkr
#15
Kristina L Schierhorn, Fabian Jolmes, Julia Bespalowa, Sandra Saenger, Christin Peteranderl, Julia Dzieciolowski, Matthias Budt, Stephan Pleschka, Andreas Herrmann, Susanne Herold, Thorsten Wolff
The RNA-dependent protein kinase (PKR) has broad antiviral activity inducing translational shut-down of viral and cellular genes and is therefore targeted by various viral proteins to facilitate pathogen propagation. The pleiotropic NS1 protein of influenza A virus acts as silencer of PKR activation and ascertains high level viral replication and virulence. However, the exact way of this inhibition remains controversial. To elucidate the structural requirements within the NS1 protein for PKR inhibition, we generated a set of mutant viruses identifying highly conserved arginine residues 35 and 46 within the NS1 N-terminus as being most critical not only for binding to and blocking activation of PKR, but also for efficient virus propagation...
March 1, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28240747/antibodies-against-nonstructural-protein-1-protect-mice-from-dengue-virus-induced-mast-cell-activation
#16
Ya-Ting Chu, Shu-Wen Wan, Yu-Chang Chang, Chien-Kuo Lee, Betty A Wu-Hsieh, Robert Anderson, Yee-Shin Lin
Dengue virus (DENV) infection causes dengue fever, dengue hemorrhagic fever (DHF), and dengue shock syndrome (DSS). DHF/DSS patients have been reported to have increased levels of urinary histamine, chymase, and tryptase, which are major granule-associated mediators from mast cells. Previous studies also showed that DENV-infected human mast cells induce production of proinflammatory cytokines and chemokines, suggesting a role played by mast cells in vascular perturbation as well as leukocyte recruitment. In this study, we show that DENV but not UV-inactivated DENV enhanced degranulation of mast cells and production of chemokines (MCP-1, RANTES, and IP-10) in a mouse model...
February 27, 2017: Laboratory Investigation; a Journal of Technical Methods and Pathology
https://www.readbyqxmd.com/read/28230408/psychometric-properties-of-adolescent-time-inventory-time-attitude-ati-ta-scores-in-three-waves-of-longitudinal-data
#17
Frank C Worrell, Michael T McKay, James R Andretta
Within the broad area of time perspective, there are numerous narrower or more specific constructs. One of these, time attitudes, is said to refer to an individual's emotional and evaluative feelings toward the past, the present, and the future. Scores on the Adolescent Time Inventory-Time Attitudes Scale (ATI-TA) have been shown to be psychometrically sound in a range of cross-sectional studies in different cultural contexts. The present study is the first longitudinal (3 waves) examination of these scores...
February 23, 2017: Psychological Assessment
https://www.readbyqxmd.com/read/28227928/pca-criterion-for-svm-mlp-classifier-for-flavivirus-biomarker-from-salivary-sers-spectra-at-febrile-stage
#18
A R M Radzol, Khuan Y Lee, W Mansor, I S Omar, A R M Radzol, Khuan Y Lee, W Mansor, I S Omar, Khuan Y Lee, A R M Radzol, I S Omar, W Mansor
Non-structural protein (NS1) has been conceded as one of the biomarkers for flavivirus that causes diseases with life threatening consequences. NS1 is an antigen that allows detection of the illness at febrile stage, mostly from blood samples currently. Our work here intends to define an optimum model for PCA-SVM with MLP kernel for classification of flavivirus biomarker, NS1 molecule, from SERS spectra of saliva, which to the best of our knowledge has never been explored. Since performance of the model depends on the PCA criterion and MLP parameters, both are examined in tandem...
August 2016: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/28221100/intracellular-degradation-and-localization-of-ns1-of-tick-borne-encephalitis-virus-affect-its-protective-properties
#19
Yulia V Kuzmenko, Elizaveta S Starodubova, Anastasia S Shevtsova, Liubov L Chernokhaeva, Anastasia A Latanova, Olga V Preobrazhenskaia, Andrey V Timofeev, Galina G Karganova, Vadim L Karpov
Currently, many DNA vaccines against infectious diseases are in clinical trials; however, their efficacy needs to be improved. The potency of DNA immunogen can be optimized by targeting technologies. In the current study, to increase the efficacy of NS1 encoded by plasmid, proteasome targeting was applied. NS1 variants with or without translocation sequence and with ornithine decarboxylase as a signal of proteasomal degradation were tested for expression, localization, protein turnover, proteasomal degradation and protection properties...
January 2017: Journal of General Virology
https://www.readbyqxmd.com/read/28220810/dengue-virus-ns1-protein-activates-immune-cells-via-tlr4-but-not-tlr2-or-tlr6
#20
Naphak Modhiran, Daniel Watterson, Antje Blumenthal, Alan G Baxter, Paul R Young, Katryn J Stacey
The secreted hexameric form of the dengue virus (DENV) non-structural protein 1 (NS1) has recently been shown to elicit inflammatory cytokine release and disrupt endothelial cell monolayer integrity. This suggests that circulating NS1 contributes to the vascular leak that plays a major role in the pathology of dengue haemorrhagic fever and shock. Pathways activated by NS1 are thus of great interest as potential therapeutic targets. Recent works have separately implicated both toll-like receptor 4 (TLR4) and the TLR2/6 heterodimer in immune cell activation by NS1...
February 21, 2017: Immunology and Cell Biology
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