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https://www.readbyqxmd.com/read/28818423/design-and-synthesis-of-multiple-antigenic-peptides-and-their-application-for-dengue-diagnosis
#1
Reeta Rai, Sameer Dubey, K V Santosh, Ashutosh Biswas, Vinit Mehrotra, D N Rao
Major difficulty in development of dengue diagnostics is availability of suitable antigens. To overcome this, we made an attempt to develop a peptide based diagnosis which offers significant advantage over other methods. With the help of in silico methods, two epitopes were selected from envelope protein and three from NS1 protein of dengue virus. These were synthesized in combination as three multiple antigenic peptides (MAPs). We have tested 157 dengue positive sera confirmed for NS1 antigen. MAP1 showed 96...
August 14, 2017: Biologicals: Journal of the International Association of Biological Standardization
https://www.readbyqxmd.com/read/28818105/severe-neutropenia-revealing-a-rare-presentation-of-dengue-fever-a-case-report
#2
J Shourick, A Dinh, M Matt, J Salomon, B Davido
BACKGROUND: Arboviruses are a common cause of fever in the returned traveler often associated with leucopenia, especially lymphopenia and thrombocytopenia. Transient neutropenia has been described in a few cases of arboviruses. However, prolonged and severe neutropenia (<500/mm(3)) has rarely been reported in dengue fever, especially in the returned traveler in Europe. CASE PRESENTATION: A 26-year-old healthy female without any medical past history, flying back from Thailand, presented a transient fever with severe neutropenia (<500/mm(3))...
August 17, 2017: BMC Research Notes
https://www.readbyqxmd.com/read/28814525/structure-guided-functional-annotation-of-the-influenza-a-virus-ns1-protein-reveals-dynamic-evolution-of-the-p85%C3%AE-binding-site-during-circulation-in-humans
#3
Antonio M Lopes, Patricia Domingues, Roland Zell, Benjamin G Hale
Rational characterization of virulence and host-adaptive markers in the multifunctional influenza A virus NS1 protein is hindered by a lack of comprehensive knowledge about NS1-host protein-protein interfaces. Here, we surveyed the impact of amino-acid variation in NS1 at its structurally-defined binding site for host p85β, a regulator of phosphoinositide-3 kinase (PI3K) signaling. Structure-guided alanine-scanning of all viral residues at this interface defined 10 positions contributing to the interaction, with residues 89, 95, 98, 133, 145 and 162 being most important...
August 16, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28814522/characterization-of-cis-acting-rna-elements-of-zika-virus-by-using-a-self-splicing-ribozyme-dependent-infectious-clone
#4
Zhong-Yu Liu, Jiu-Yang Yu, Xing-Yao Huang, Hang Fan, Xiao-Feng Li, Yong-Qiang Deng, Xue Ji, Meng-Li Cheng, Qing Ye, Hui Zhao, Jian-Feng Han, Xiao-Ping An, Tao Jiang, Bo Zhang, Yi-Gang Tong, Cheng-Feng Qin
Zika virus (ZIKV) has caused significant outbreaks and epidemics in the Americas recently, raising global concern due to its ability to cause microcephaly and other neurological complications. A stable and efficient infectious clone of ZIKV is urgently needed. However, the instability and toxicity of flavivirus cDNA clones in E. coli hosts has hindered the development of ZIKV infectious clones. Herein, using a novel self-splicing ribozyme-based strategy, we have generated a stable infectious cDNA clone of a contemporary ZIKV strain imported from Venezuela to China in 2016...
August 16, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28806616/parvovirus-b19-integration-into-human-cd36-erythroid-progenitor-cells
#5
Tyler Janovitz, Susan Wong, Neal S Young, Thiago Oliveira, Erik Falck-Pedersen
The pathogenic autonomous human parvovirus B19 (B19V) productively infects erythroid progenitor cells (EPCs). Functional similarities between B19V nonstructural protein (NS1), a DNA binding endonuclease, and the Rep proteins of Adeno-Associated Virus (AAV) led us to hypothesize that NS1 may facilitate targeted nicking of the human genome and B19 vDNA integration. We adapted an integration capture sequencing protocol (IC-Seq) to screen B19V infected human CD36+ EPCs for viral integrants, and discovered 40,000 unique B19V integration events distributed throughout the human genome...
August 11, 2017: Virology
https://www.readbyqxmd.com/read/28796576/antibody-affinity-against-2009-a-h1n1-influenza-and-pandemrix-vaccine-nucleoproteins-differs-between-childhood-narcolepsy-patients-and-controls
#6
Alexander Lind, Eva Freyhult, Anita Ramelius, Tomas Olsson, Lisen Arnheim-Dahlström, Favelle Lamb, Mohsen Khademi, Aditya Ambati, Markus Maeurer, Izaura Lima Bomfim, Katharina Fink, Malin Fex, Carina Törn, Helena Elding Larsson, Åke Lernmark
Increased narcolepsy incidence was observed in Sweden following the 2009 influenza vaccination with Pandemrix(®). A substitution of the 2009 nucleoprotein for the 1934 variant has been implicated in narcolepsy development. The aims were to determine (a) antibody levels toward wild-type A/H1N1-2009[A/California/04/2009(H1N1)] (NP-CA2009) and Pandemrix-[A/Puerto Rico/8/1934(H1N1)] (NP-PR1934) nucleoproteins in 43 patients and 64 age-matched controls; (b) antibody affinity in reciprocal competitive assays in 11 childhood narcolepsy patients compared with 21 age-matched controls; and (c) antibody levels toward wild-type A/H1N1-2009[A/California/04/2009(H1N1)] (H1N1 NS1), not a component of the Pandemrix vaccine...
August 10, 2017: Viral Immunology
https://www.readbyqxmd.com/read/28791003/dengue-virus-glycosylation-what-do-we-know
#7
REVIEW
Sally S L Yap, Terry Nguyen-Khuong, Pauline M Rudd, Sylvie Alonso
In many infectious diseases caused by either viruses or bacteria, pathogen glycoproteins play important roles during the infection cycle, ranging from entry to successful intracellular replication and host immune evasion. Dengue is no exception. Dengue virus glycoproteins, envelope protein (E) and non-structural protein 1 (NS1) are two popular sub-unit vaccine candidates. E protein on the virion surface is the major target of neutralizing antibodies. NS1 which is secreted during DENV infection has been shown to induce a variety of host responses through its binding to several host factors...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28780429/pro-inflammatory-cytokine-responses-in-human-gingival-epithelial-cells-after-stimulation-with-cell-wall-extract-of-aggregatibacter-actinomycetemcomitans-subtypes
#8
Nuntiya Pahumunto, Pareena Chotjumlong, Anupong Makeudom, Suttichai Krisanaprakornkit, Gunnar Dahlen, Rawee Teanpaisan
Varying cytokine responses of human gingival epithelial cells (HGECs) by Aggregatibacter actinomycetemcomitans subtypes have been found. Most studies have used reference strains, whereas a few has evaluated the cytokine expression in response to clinical subtypes of this bacterial species. This study aimed to examine whether there was any difference in cytokine responses of HGECs stimulated with cell wall extract (CWE) from A. actinomycetemcomitans subtypes included clinical strains from Thai adult periodontitis, various serotypes and non-serotypeable strains, strains from deep or shallow pockets, and reference serotype strains...
August 3, 2017: Anaerobe
https://www.readbyqxmd.com/read/28777951/human-bocavirus-1-infection-of-caco-2-cell-line-cultures
#9
Lucía María Ghietto, Ana Paola Toigo D'Angelo, Franco Agustin Viale, María Pilar Adamo
Human bocavirus 1 (HBoV1) is a parvovirus associated with pneumonia in infants. It has been detected in different tissues, including colorectal tumors. In this study, we investigated whether Caco-2 cell line, derived from human colon cancer, can be utilized as a model for HBoV1 replication. We demonstrate HBoV1 replication in Caco-2 cultures supplemented with DEAE-dextran after inoculation with respiratory material from infected patients presenting with acute respiratory infection. A viral cycle of rapid development is displayed...
August 1, 2017: Virology
https://www.readbyqxmd.com/read/28770247/age-of-initial-cohort-of-dengue-patients-could-explain-the-origin-of-disease-outbreak-in-a-setting-a-case-control-study-in-rajasthan-india
#10
Annette Angel, Bennet Angel, Karuna Yadav, Neha Sharma, Vinod Joshi, Indu Thanvi, Sharad Thanvi
Dengue fever (DF) and dengue hemorrhagic fever (DHF) is a public health problem with 390 million cases reported in world annually. In Rajasthan, DF with DHF is being reported for about two decades. For undertaking interventions into disease transmission, locating origin of transmission is very important. Present paper reports retrospective analysis of the hospital reported cases of dengue during the year 2013-2014 undertaken in Barmer, Rajasthan. To address task of investigating outbreak, detailed analysis of the data on serological test results (Mac-ELISA assay of NS1, IgG and IgM) performed by local hospital, Balotra was made...
June 2017: Virusdisease
https://www.readbyqxmd.com/read/28765967/influenza-virus-non%C3%A2-structural-protein-1-inhibits-the-production-of-interferon-%C3%AE-of-alveolar-epithelial-cells-upon-the-infection-of-influenza-a-h1n1
#11
Hao Jiang, Si-Mei Shen, Jie Yin, Peng-Peng Zhang, Yi Shi
Influenza A affects a large population worldwide. Influenza virus evades immune responses via various mechanisms, including through the modification of the immune microenvironment. Influenza virus non‑structural protein 1 (NS1) encoded by the virus genome inhibits type I interferon (IFN) signaling pathways, which is essential for viral clearance. However, the precise mechanisms of NS1‑mediated immune suppression remain poorly understood. The results of the present study demonstrated that mice infected with NS1‑expressing influenza A H1N1 virus had lower expression levels of IFNβ in the lung...
August 2, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28765561/antibodies-against-modified-ns1-wing-domain-peptide-protect-against-dengue-virus-infection
#12
Yen-Chung Lai, Yung-Chun Chuang, Ching-Chuan Liu, Tzong-Shiann Ho, Yee-Shin Lin, Robert Anderson, Trai-Ming Yeh
Dengue is the most common mosquito-transmitted viral infection for which an improved vaccine is still needed. Although nonstructural protein-1 (NS1) immunization can protect mice against dengue infection, molecular mimicry between NS1 and host proteins makes NS1-based vaccines challenging to develop. Based on the epitope recognized by the anti-NS1 monoclonal Ab (mAb) 33D2 which recognizes a conserved NS1 wing domain (NS1-WD) region but not host proteins, we synthesized a modified NS1-WD peptide to immunize mice...
August 1, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28758625/the-dengue-virus-non-structural-protein-1-ns1-is-secreted-from-infected-mosquito-cells-via-a-non-classical-caveolin-1-dependent-pathway
#13
Ana C Alcalá, Raiza Hernández-Bravo, Fernando Medina, David S Coll, Jose L Zambrano, Rosa M Del Angel, Juan E Ludert
Dengue virus NS1 is a glycoprotein of 46-50 kDa that is conserved among flaviviruses, associates as a dimer to cell membranes and is secreted as a hexamer to the extracellular milieu. Recent evidence showed that NS1 is secreted efficiently from infected mosquito cells. To explore the secretory route of NS1 in mosquito cells, infected cells were treated with brefeldin A (BFA) and methyl-beta-cyclodextrin (MβCD). The results showed that MβCD, but not BFA, significantly reduced the release of NS1. Moreover, silencing the expression of caveolin-1 (CAV1; a key component of the caveolar system that transports cholesterol inside the cell), but not SAR1 (a GTPase that participates in the classical secretory pathway), also results in a significant reduction of the secretion of NS1...
July 31, 2017: Journal of General Virology
https://www.readbyqxmd.com/read/28757142/influenza-virus-ns1-protein-mutations-at-position-171-impact-innate-interferon-responses-by-respiratory-epithelial-cells
#14
Ewan P Plant, Natalia A Ilyushina, Faruk Sheikh, Raymond P Donnelly, Zhiping Ye
The influenza virus NS1 protein interacts with a wide range of proteins to suppress the host cell immune response and facilitate virus replication. The amino acid sequence of the 2009 pandemic virus NS1 protein differed from sequences of earlier related viruses. The functional impact of these differences has not been fully defined. Therefore, we made mutations to the NS1 protein based on these sequence differences, and assessed the impact of these changes on host cell interferon (IFN) responses. We found that viruses with mutations at position 171 replicated efficiently but did not induce expression of interferon genes as effectively as wild-type viruses in A459 lung epithelial cells...
July 27, 2017: Virus Research
https://www.readbyqxmd.com/read/28756561/evidence-of-dengue-and-chikungunya-virus-co-infection-and-circulation-of-multiple-dengue-serotypes-in-a-recent-indian-outbreak
#15
S Mukherjee, S K Dutta, S Sengupta, A Tripathi
In India, dengue endemic areas overlap with chikungunya-affected areas and both the viruses are transmitted by same vector, Aedes aegypti - thereby increasing opportunity of co-infection by both viruses. Present study was carried out to understand the DENV-CHIKV infection dynamics during recent outbreaks in eastern India (West Bengal state) and its implication on disease manifestations. Blood was collected from 326 symptomatic febrile patients. Patients' serum was subjected to serological diagnosis for presence of anti-dengue-IgM, anti-chikungunya-IgM antibodies and dengue-NS1 antigen by ELISA...
July 29, 2017: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/28749765/generation-and-characterization-of-antinonstructural-protein-1-monoclonal-antibodies-and-development-of-diagnostics-for-dengue-virus-serotype-2
#16
Yin-Liang Tang, I-Ju Liu, Pi-Chun Li, Chien-Yu Chiu, Chun-Yu Lin, Chung-Hao Huang, Yen-Hsu Chen, Chi-Yu Fu, Day-Yu Chao, Chwan-Chuen King, Han-Chung Wu
Dengue virus (DENV) circulates in tropical and subtropical areas around the world, where it causes high morbidity and mortality. There is no effective treatment of infection, with supportive care being the only option. Furthermore, early detection and diagnosis are important to facilitate clinical decisions. In this study, seven monoclonal antibodies (mAbs) recognizing nonstructural protein 1 (NS1) of DENV were generated by hybridoma techniques. These antibodies can be divided into two groups: serotype-specific (DB6-1, DB12-3, and DB38-1) and nonspecific (consisting of antibodies DB16-1, DB20-6, DB29-1, and DB41-2)...
July 24, 2017: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/28746401/identification-of-one-b-cell-epitope-from-ns1-protein-of-duck-tembusu-virus-with-monoclonal-antibodies
#17
Jinfeng Ti, Zhijie Li, Xiuli Li, Yunjian Lu, Youxiang Diao, Fang Li
This study describes the identification of one linear B-cell epitope on TMUV NS1 protein with monoclonal antibody (mAb) 3G2 by indirect enzyme-linked immunosorbent assay (ELISA). In this study, NS1 protein was expressed in prokaryotic expression system and purified. One mAb against NS1 protein was generated from Balb/c mice immunized with recombinant protein NS1. A set of 35 partially-overlapping polypeptides covering the entire NS1 protein was expressed with PGEX-6P-1 vector and screened with mAb 3G2. One polypeptide against the mAb was acquired and identified by indirect ELISA and western-blot...
2017: PloS One
https://www.readbyqxmd.com/read/28744070/mri-features-in-dengue-encephalitis-a-case-series-in-south-indian-tertiary-care-hospital
#18
Brijesh K Soni, Debasheesh S R Das, R A George, R Aggarwal, R Sivasankar
Dengue virus, a RNA virus of family Flaviviradae is considered non-neurotropic. Increasing studies and case reports reveal neurological manifestations of dengue virus. In our case series, we have evaluated magnetic resonance imaging (MRI) findings of 3 patients with dengue fever diagnosed by positive dengue NS1 antigen with neurological symptoms, which revealed nonspecific imaging features of dengue encephalitis in two cases and dengue meningoencephalitis in one case. Autopsy findings are also correlated in 2 patients who succumbed to their disease...
April 2017: Indian Journal of Radiology & Imaging
https://www.readbyqxmd.com/read/28740517/dengue-virus-ns1-protein-as-a-diagnostic-marker-commercially-available-elisa-and-comparison-to-qrt-pcr-and-serological-diagnostic-assays-currently-used-by-the-state-of-florida
#19
Jason H Ambrose, Shamala Devi Sekaran, Azliyati Azizan
BACKGROUND: The proper management of patients infected with dengue virus requires early detection. Here, real-time molecular assays have proven useful but have limitations, whereas ELISAs that detect antibodies are still favored but results are obtained too late to be of clinical value. The production of DENV NS1 peaks early during infection and its detection can combine the advantages of both diagnostic approaches. METHODS: This study compared assays currently used for detecting DENV infection at the Florida Department of Health including anti-DENV IgM and IgG ELISAs as well as qRT-PCR, against a commercially available DENV NS1 ELISA...
2017: Journal of Tropical Medicine
https://www.readbyqxmd.com/read/28726595/co-circulation-and-co-infections-of-all-dengue-virus-serotypes-in-hyderabad-india-2014
#20
K Vaddadi, C Gandikota, P K Jain, V S V Prasad, M Venkataramana
The burden of dengue virus infections increased globally during recent years. Though India is considered as dengue hyper-endemic country, limited data are available on disease epidemiology. The present study includes molecular characterization of dengue virus strains occurred in Hyderabad, India, during the year 2014. A total of 120 febrile cases were recruited for this study, which includes only children and 41 were serologically confirmed for dengue positive infections using non-structural (NS1) and/or IgG/IgM ELISA tests...
September 2017: Epidemiology and Infection
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