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Eléonore M'baya-Moutoula, Loïc Louvet, Roland Molinié, Ida Chiara Guerrera, Catherine Cerutti, Ophélie Fourdinier, Virginie Nourry, Laurent Gutierrez, Patrice Morlière, François Mesnard, Ziad A Massy, Valérie Metzinger-le Meuth, Laurent Metzinger
No abstract text is available yet for this article.
May 17, 2018: Biochimica et Biophysica Acta
Wenjian Xie, Qian Yin, Mengran Zhang, Shengnan Li, Shaoliang Chen
To investigate the potential correlation between miR-223 level in leukocytes and platelet responses to clopidogrel in patients with coronary artery disease.
 Methods: A cohort of 188 outpatients, who conducted percutaneous coronary intervention (PCI) and received dual antiplatelet therapy, were recruited. The patient's electronic health data were collected, and their blood samples were obtained for measurement of adenosine diphosphate (ADP)-induced whole-blood platelet aggregation. Extreme cases of platelet responses to clopidogrel (ultra- vs...
April 28, 2018: Zhong Nan da Xue Xue Bao. Yi Xue Ban, Journal of Central South University. Medical Sciences
Yong Zhang, Ailiang Zeng, Shuheng Liu, Rui Li, Xiefeng Wang, Wei Yan, Hailin Li, Yongping You
MicroRNAs (miRNA) regulate a number of cellular processes. Recent studies have indicated that these molecules function in the epithelial-mesenchymal transition (EMT). However, the crucial systematic role of EMT and miRNAs together in glioblastoma (GBM) remains poorly understood. The present study demonstrated that EMT was closely associated with malignant progression and clinical outcome using three independent glioma databases (GSE16011, Rembrandt and The Cancer Genome Atlas). Furthermore, integrated analysis of miRNAs and mRNA profiling in 491 GBM samples revealed an EMT biological process associated with an miRNA profile (19 positively and 18 negatively correlated miRNAs)...
May 2018: Oncology Letters
Chundi Gao, Chao Zhou, Jing Zhuang, Lijuan Liu, Cun Liu, Huayao Li, Gongxi Liu, Junyu Wei, Changgang Sun
Growing evidence has shown that a large number of miRNAs are abnormally expressed in cervical cancer (CC) tissues and play irreplaceable roles in tumorigenesis, progression, and metastasis. This study aimed to identify new biomarkers and pivotal genes associated with CC prognosis through comprehensive bioinformatics analysis. At first, the data of gene expression microarray (GSE30656) was downloaded from GEO database and differential miRNAs were obtained. Additionally, 4 miRNAs associated with the survival time of patients with CC were screened through TCGA differential data analysis, Kaplan-Meier, and Landmark analysis...
May 8, 2018: Journal of Cellular Biochemistry
Cheng Zhang, Jiamin Zhang
Hepatocellular carcinoma (HCC) is one of the most harmful types of cancer. Previous studies have demonstrated that microRNA (miR)-223 is downregulated in the serum and tumor tissue of patients with HCC. The present study aimed to investigate the regulatory role of miR-223 on insulin-like growth factor-1 receptor (IGF-1R) and downstream factors in HCC. The Hep3B cell line was transfected with miR-223 mimic and inhibitor. Following transfection, cell proliferation was analyzed using a cell counting kit 8 assay and cellular apoptosis was assessed using flow cytometry...
May 2018: Experimental and Therapeutic Medicine
Vincenzo Grassia, Angela Lombardi, Hiromichi Kawasaki, Carmela Ferri, Letizia Perillo, Laura Mosca, Donatella Delle Cave, Ludovica Nucci, Marina Porcelli, Michele Caraglia
MicroRNAs (miRNAs) are endogenous non-coding RNAs of about twenty-two nucleotides that regulate gene expression through post-transcriptional control. The purpose of the present study was to identify and describe the salivary miRNAs in cleft lip and palate (CLP) patients comparing them with a control healthy group. Twelve patients (mean age 11.9 ± 2.42 years; 6M/6F) formed the study group. The control group was created selecting twelve healthy subjects matched for age and sex with study group. We recorded differences in miRNA expression profile between the saliva of CLP patients and the control group...
April 10, 2018: Oncotarget
Chao Liu, Zuozhang Yang, Zhiyong Deng, Youjun Zhou, Quan Gong, Ruilian Zhao, Ting Chen
In this study, we aimed at investigating effects of lncRNA ADAMTS9-AS2 on lung cancer progression through regulating miR-223-3p and TGFBR3 expressions. Expressions of ADAMTS9-AS2 in lung cancer tissues and cell lines were determined by reverse transcriptase polymerase chain reaction (qRT-PCR). TargetScan and miRcode were used to predict the targeting relationships, respectively. The luciferase reporter system was used to verify that the relationship among ADAMTS9-AS2, TGFBR3 and miR-223-3p. Western blot assay tested the protein level changes in TGFBR3...
April 29, 2018: IUBMB Life
Hua Wang, Pan Hao, Hu Zhang, Cuiping Xu, Junyan Zhao
This study was aimed to research the effect of miR-223 on the inflammatory responses induced by lipopolysaccharide (LPS) in nucleus pulposus (NP) cells of rat intervertebral disc. Isolated rat NP cells were induced by LPS. Reverse transcriptase quantitative real-time polymerase chain reaction was used to detect gene expression. To detect protein expression, Western blot and enzyme-linked immunosorbent assay experiments were applied. The putative targeting relationship between miR-223 and Irak1 was determined using dual-luciferase reporter gene assay...
April 29, 2018: IUBMB Life
Hiroaki Ishikawa, Hiroya Yamada, Kanako Kondo, Takeru Ota, Mirai Yamazaki, Yoshitaka Ando, Genki Mizuno, Eiji Munetsuna, Koji Suzuki, Ryoji Teradaira, Koji Ohashi
BACKGROUND: MicroRNAs are present not only in exosomes but also in high-density lipoprotein (HDL) and have the potential as biomarkers for various diseases. Various purification methods have been developed to quantify HDL-miRNAs; however, they are unsuitable for clinical applications. Therefore, we aimed to establish a simpler analytical method to quantify HDL-miRNAs for clinical applications. METHODS: We purified HDL fraction from pooled plasma using a three-step protocol consisting of ultracentrifugation, phosphotungstic acid/MgCl2 precipitation, and desalting/buffer exchange followed by the quantification of HDL-miRNAs by qRT-PCR...
January 1, 2018: Annals of Clinical Biochemistry
Jieqiong Ding, Zhiwei Zhao, Jia Song, Binghua Luo, Lingping Huang
Although doxorubicin has become a key drug in cancer treatment, the resistance of colorectal carcinoma to doxorubicin is a major problem in clinical practice. F-box and WD repeat domain-containing 7 (FBXW7) plays important roles in human cancers and is one of the major causes of drug resistance. The miR-223/FBXW7 pathway has been reported to be a crucial clue to the mechanism of chemoresistance in many human cancers, such as gastric cancer, breast cancer, and non-small cell lung cancer. However, it is unclear whether similar mechanisms of doxorubicin resistance are involved in colorectal cancer (CRC)...
April 26, 2018: Acta Biochimica et Biophysica Sinica
Xiaoxiao Liu, Yifeng Xu, Yunfei Deng, Hongli Li
BACKGROUND/AIMS: Percutaneous coronary intervention reduces acute myocardial infarction (MI)-induced mortality to a great extent, but effective treatments for MI-induced cardiac fibrosis and heart failure are still lacking. MicroRNAs (miRNAs) play a variety of roles in cells and have thus been investigated extensively. MicroRNA-223 (miR-223) expression has been reported to be altered in post-MI heart failure in humans; however, the roles of miR-223 in MI remain unknown. Our study aimed to elucidate the roles of miR-223 in cardiac fibrosis...
April 19, 2018: Cellular Physiology and Biochemistry
P Micó-Martínez, J-L García-Giménez, M Seco-Cervera, A López-Roldán, P-J Almiñana-Pastor, F Alpiste-Illueca, F-V Pallardó
BACKGROUND: The study and identification of new biomarkers for periodontal disease, such as microRNAs (miRNAs), may give us more information about the location and severity of the disease and will serve as a basis for treatment planning and disease-monitoring. miRNAs are a group of small RNAs which are involved in gene regulation by binding to their messenger RNA target (mRNA). In this pilot study, the procedure for purifying miRNAs from gingival crevicular fluid (GCF) was, for the first time, described...
May 1, 2018: Medicina Oral, Patología Oral y Cirugía Bucal
Jerome P Richie
No abstract text is available yet for this article.
May 2018: Journal of Urology
Katharina Schönauen, Nha Le, Ulrike von Arnim, Christian Schulz, Peter Malfertheiner, Alexander Link
Background: Assessment of the disease activity in inflammatory bowel disease (IBD) is essential for adequate treatment management and reliable noninvasive biomarkers for verification of mucosal healing are still needed. MicroRNAs (miRNAs) are differentially expressed in IBD and cancer. We aimed to evaluate the potential of circulating and fecal miRNAs as diagnostic biomarkers for IBD. Methods: In this proof-of-principle study we used 2 independent patient cohorts...
April 12, 2018: Inflammatory Bowel Diseases
She-Jun Feng, Xue-Qiang Zhang, Jun-Tao Li, Xiao-Min Dai, Fei Zhao
Cerebral ischemia injury seriously endangers human health and its molecular mechanism is still not fully understood. microRNA-223 (miR-223) has been reported to be involved in many physiological functions but the specific role of miRNA-223 in ischemic neuronal injury is still unclear. An oxygen-glucose deprivation and simulated reperfusion (OGD/R) model was constructed here to investigate the possible role miR-223 played in ischemic neuronal injury. The expression of miRNA-223 in the OGD/R model and its effect on cell proliferation were studied by qPCR and CCK8 assay...
2018: Folia Neuropathologica
Li-Juan Wei, Jun-An Li, Dong-Mei Bai, Ying Song
MicroRNAs (miRNAs) can function as tumor suppressor or oncogenic genes. The putative targets of miR-223 include tumor suppressor gene, RhoB. Here we sought to investigate the role of miR-223-RhoB signaling pathway in proliferation of colon cancer. We used Western blot, immunofluorescence staining, or RT-PCR to detect expression levels of miR-223 and RhoB in colon adenocarcinoma and adjacent non-cancerous tissue samples, or in human colon adenocarcinoma cell lines. MTT assay was used to determine proliferation and apoptosis in cell lines...
April 13, 2018: Chemico-biological Interactions
Quanbo Ji, Xiaojie Xu, Qi Song, Yameng Xu, Yanhong Tai, Stuart B Goodman, Wenzhi Bi, Meng Xu, Shunchang Jiao, William J Maloney, Yan Wang
Cadherin-6 (CDH6) is aberrantly expressed in cancer and closely associated with tumor progression. However, the functions of CDH6 in human osteosarcoma and the molecular mechanisms underlying CDH6 in osteosarcoma oncogenesis remain poorly understood. In this work, we assessed the role of CDH6 in human osteosarcoma and identified that the expression of CDH6 was closely related with the overall survival and poor prognosis of osteosarcoma patients. MicroRNAs (miRNAs) have been implicated as important epigenetic regulators during the progression of osteosarcoma...
March 14, 2018: Molecular Therapy: the Journal of the American Society of Gene Therapy
Justyna Pordzik, Katarzyna Pisarz, Salvatore De Rosa, Axel Dyve Jones, Ceren Eyileten, Ciro Indolfi, Lukasz Malek, Marek Postula
Platelet activation plays a pivotal role in the development and progression of atherosclerosis, which often leads to potentially fatal ischemic events at later stages of the disease. Platelets and platelet microvesicles (PMVs) contain large amounts of microRNA (miRNA), which contributes largely to the pool of circulating miRNAs. Hence, they represent a promising option for the development of innovative diagnostic biomarkers, that can be specific for the underlying etiology. Circulating miRNAs can be responsible for intracellular communication and may have a biological effect on target cells...
2018: Frontiers in Endocrinology
Liyan Dou, Kaiyu Han, Mochao Xiao, Fuzhen Lv
MiR-223-5p has been demonstrated to regulate the development and progression of various cancers, such as hepatocellularcarcinoma, breast cancer and gastric carcinoma. However, the role of miR-223-5p in non-small cell lung cancer (NSCLC) requires further investigation. In this study, we found that the expression of miR-223-5p was significantly down-regulated in NSCLC tissues and cell lines. Moreover, the expression level of miR223-5p is negatively correlated with the malignance of NSCLC. Besides, we found that overexpression of miR-223-5p remarkably suppressed the proliferation of NSCLC cells in vitro and in vivo ...
April 3, 2018: Oncology Research
Xiaoxiao Liu, Yunfei Deng, Yifeng Xu, Wei Jin, Hongli Li
Myocardial infarction (MI), characterized by interruption of blood and oxygen to myocardium, is a common yet fatal cardiovascular event that causes progressive damage to myocardial tissue and eventually leads to heart failure. Previous studies have shown increased expression of microRNA-223 (miR-223) in infarcted myocardial tissues of humans and in rat models of MI. However, the role of miR-223 in cell survival during MI has not been elucidated. Thus, we aimed to investigate whether miR-223 participates in the regulation of cardiac ischemia-induced injury and to elucidate the underlying mechanisms of this process...
May 2018: Journal of Molecular and Cellular Cardiology
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