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https://www.readbyqxmd.com/read/28525289/high-throughput-phenotyping-of-human-induced-pluripotent-stem-cell-derived-cardiomyocytes-and-neurons-using-electric-field-stimulation-and-high-speed-fluorescence-imaging
#1
Neil J Daily, Zhong-Wei Du, Tetsuro Wakatsuki
Electrophysiology of excitable cells, including muscle cells and neurons, has been measured by making direct contact with a single cell using a micropipette electrode. To increase the assay throughput, optical devices such as microscopes and microplate readers have been used to analyze electrophysiology of multiple cells. We have established a high-throughput (HTP) analysis of action potentials (APs) in highly enriched motor neurons and cardiomyocytes (CMs) that are differentiated from human induced pluripotent stem cells (iPSCs)...
May 19, 2017: Assay and Drug Development Technologies
https://www.readbyqxmd.com/read/28521159/distribution-and-genetic-diversity-of-microbial-populations-in-the-pilot-scale-biofilter-for-simultaneous-removal-of-ammonia-iron-and-manganese-from-real-groundwater
#2
Qingfeng Cheng, Lichao Nengzi, Linlin Bao, Yang Huang, Shengyu Liu, Xiuwen Cheng, Bo Li, Jie Zhang
A pilot-scale biofilter treating real groundwater was developed in this study, which showed that ammonia, iron and manganese were mainly removed at 0.4, 0.4 and 0.8 m of the filter bed, respectively, and the corresponding removal efficiencies were 90.82%, 95.48% and 95.90% in steady phase, respectively. The variation of microbial populations in the biofilter during start-up process was also investigated using high-throughput pyrosequencing (HTP). Results indicated that the main functional microbes for ammonia, iron and manganese removal were Nitrosomonas, Crenothrix and Crenothrix, respectively, which was mainly distributed at 0...
May 13, 2017: Chemosphere
https://www.readbyqxmd.com/read/28513694/atomic-scale-observation-of-a-defect-mediated-first-order-phase-transition-in-vo2-a
#3
Chao Jiang, Lu Chen, Shidong Ji, Jun Liu, Zhengfei Zhang, Ping Jin, Yong Wang, Ze Zhang
The study of first-order structural transformations has attracted extensive attention due to their significant scientific and industrial importance. However, it remains challenging to exactly determine the nucleation sites at the very beginning of the transformation. Here, we report the atomic scale real-time observation of a unique defect-mediated reversible phase transition between the low temperature phase (LTP) and the high temperature phase (HTP) of VO2(A). In situ Cs-corrected scanning transmission electron microscopy (STEM) images clearly indicate that both phase transitions (from the HTP to the LTP and from the LTP to the HTP) start at the defect sites in parent phases...
May 17, 2017: Nanoscale
https://www.readbyqxmd.com/read/28502117/passive-membrane-penetration-of-the-serotonin-precursor-5-hydroxytryptophan-is-controlled-by-its-zwitterion
#4
Károly Mazák, Béla Noszál
Species-specific partition coefficients in the octanol/water system were determined for the neurotransmitter serotonin (5-HT) and its precursor 5-hydroxytryptophan (5-HTP). The pH-independent partition coefficients (p) of the individual microspecies were determined by combination of experimentally measured distribution constants and a custom-tailored evaluation method, using highly similar auxiliary compounds. Experimental microscopic partition coefficients for triprotic molecules have only been reported before for thyroxine and its derivatives...
May 14, 2017: Chemistry & Biodiversity
https://www.readbyqxmd.com/read/28489830/-clinical-and-laboratory-characteristics-of-patients-with-pulmonary-hypertension-and-pulmonary-vascular-complications-hospitalized-at-the-instituto-nacional-de-salud-del-ni%C3%A3-o
#5
Alexis Jose Ormeño Julca, Carlos Melchor Alvarez Murillo, Pedro Miguel Amoretti Alvino, Angel Aladino Florian Florian, Rosa Aurora Castro Johanson, Maria Danisa Celi Perez, Olga Rocío Huamán Prado
The hepatopulmonary syndrome (HPS) and portopulmonary hypertension (PPHN) are distinct pulmonary vascular complications of portal hypertension (PHT) and are associated with increased morbidity and mortality. OBJECTIVES: To describe the clinical and laboratory characteristics of patients with pulmonary hypertension and pulmonary vascular complications hospitalized at the Instituto Nacional de Salud del Niño. MATERIALS AND METHODS: We included patients with HTP hospitalized from January 2012 to June 2013 and that during its evolution progressed with SHP or HTPP...
January 2017: Revista de Gastroenterología del Perú: órgano Oficial de la Sociedad de Gastroenterología del Perú
https://www.readbyqxmd.com/read/28485580/solution-processed-organic-and-halide-perovskite-transistors-on-hydrophobic-surfaces
#6
Jeremy W Ward, Hannah Smith, Andrew Zeidell, Peter Diemer, Stephen Baker, Hyunsu Lee, Marcia M Payne, John E Anthony, Martin Guthold, Oana D Jurchescu
Solution-processable electronic devices are highly desirable due to their low cost and compatibility with flexible substrates. However, they are often challenging to fabricate due to the hydrophobic nature of the surfaces of the constituent layers. Here, we use a protein solution to modify the surface properties and to improve the wettability of the fluoropolymer dielectric Cytop®. The engineered hydrophilic surface is successfully incorporated in bottom-gate solution deposited organic field-effect transistors (OFETs) and organic-inorganic halide perovskite field-effect transistors (HTP-FETs) fabricated on flexible substrates...
May 9, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28456683/patchouli-alcohol-ameliorates-dextran-sodium-sulfate-induced-experimental-colitis-and-suppresses-tryptophan-catabolism
#7
Chang Qu, Zhong-Wen Yuan, Xiu-Ting Yu, Yan-Feng Huang, Guang-Hua Yang, Jian-Nan Chen, Xiao-Ping Lai, Zi-Ren Su, Hui-Fang Zeng, Ying Xie, Xiao-Jun Zhang
Despite the increased morbidity of ulcerative colitis (UC) in recent years, available treatments remain unsatisfactory. Pogostemon cablin has been widely applied to treat a variety of gastrointestinal disorders in clinic for centuries, in which patchouli alcohol (PA, C15H26O) has been identified as the major active component. This study attempted to determine the bioactivity of PA on dextran sulfate sodium (DSS)-induced mice colitis and clarify the mechanism of action. Acute colitis was induced in mice by 3% DSS for 7 days...
April 27, 2017: Pharmacological Research: the Official Journal of the Italian Pharmacological Society
https://www.readbyqxmd.com/read/28447897/specific-hepatic-stellate-cell-penetrating-peptide-targeted-delivery-of-a-kla-peptide-reduces-collagen-accumulation-by-inducing-apoptosis
#8
Zhengrong Guo, Dong Li, Huanyan Peng, Jiwen Kang, Xiaoyu Jiang, Xiaoli Xie, Dianxing Sun, Huijing Jiang
Liver fibrosis is an aberrant wound-healing process to chronic hepatic inflammation and is characterized by excessive accumulation of extracellular matrix (ECM) that is produced by activated hepatic stellate cells (HSCs). Thus, activated HSCs play a key role in the pathogenesis of liver fibrosis and are a potential target for the treatment of liver fibrosis. Herein, we report that a specific HSC-penetrating peptide reduced collagen accumulation by inducing the apoptosis of HSC-T6 cells. We first screened HSC-specific transduction peptides and identified a novel HSC-targeted cell-penetrating peptide (HTP) that specifically interacted with HSC-T6 cells...
April 27, 2017: Journal of Drug Targeting
https://www.readbyqxmd.com/read/28434742/endocrine-and-metabolic-changes-in-transition-dairy-cows-are-affected-by-prepartum-infusions-of-a-serotonin-precursor
#9
Lorenzo E Hernández-Castellano, Laura L Hernandez, Helga Sauerwein, Rupert M Bruckmaier
Serotonin (5-HT) has been shown to be involved in calcium homeostasis, modulating calcium concentration in blood. In addition, 5-HT participates in a variety of metabolic pathways, mainly through the modulation of glucose and lipid metabolism. The hypothesis of the present study was that the prepartum administration of 5-hydroxy-l-tryptophan (5-HTP), a 5-HT precursor, would affect endocrine systems related to calcium homeostasis, and interact with other endocrine and metabolic pathways during the transition period...
April 20, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28423797/htp-nlp-a-new-nlp-system-for-high-throughput-phenotyping
#10
Daniel R Schlegel, Chris Crowner, Frank Lehoullier, Peter L Elkin
Secondary use of clinical data for research requires a method to quickly process the data so that researchers can quickly extract cohorts. We present two advances in the High Throughput Phenotyping NLP system which support the aim of truly high throughput processing of clinical data, inspired by a characterization of the linguistic properties of such data. Semantic indexing to store and generalize partially-processed results and the use of compositional expressions for ungrammatical text are discussed, along with a set of initial timing results for the system...
2017: Studies in Health Technology and Informatics
https://www.readbyqxmd.com/read/28413824/perturbation-of-serotonin-homeostasis-during-adulthood-affects-serotonergic-neuronal-circuitry
#11
Marta Pratelli, Sara Migliarini, Barbara Pelosi, Francesco Napolitano, Alessandro Usiello, Massimo Pasqualetti
Growing evidence shows that the neurotransmitter serotonin (5-HT) modulates the fine-tuning of neuron development and the establishment of wiring patterns in the brain. However, whether serotonin is involved in the maintenance of neuronal circuitry in the adult brain remains elusive. Here, we use a Tph2(fl)°(x) conditional knockout (cKO) mouse line to assess the impact of serotonin depletion during adulthood on serotonergic system organization. Data show that the density of serotonergic fibers is increased in the hippocampus and decreased in the thalamic paraventricular nucleus (PVN) as a consequence of brain serotonin depletion...
March 2017: ENeuro
https://www.readbyqxmd.com/read/28413401/parkinsonism-in-association-with-dihydropteridine-reductase-deficiency
#12
Yoshiaki Takahashi, Yasuhiro Manabe, Yumiko Nakano, Taijun Yunoki, Syoichiro Kono, Hisashi Narai, Mahoko Furujo, Koji Abe
We report a 16-year-old man with disorders of tetrahydrobiopterin metabolism due to dihydropteridine reductase (DHPR) deficiency. He revealed moderate mental retardation, parkinsonism, and spastic paralysis with levodopa and 5-hydroxytryptophan (5-HTP) supplementation from the age of 2 months. Brain MRI showed high intensity areas in bilateral frontal and posterior deep white matter on fluid-attenuated inversion recovery (FLAIR). Coronal FLAIR image showed a high signal in bilateral pyramidal tracts. Single photon computed tomography (SPECT) imaging of the dopamine transporter was normal...
January 2017: Case Reports in Neurology
https://www.readbyqxmd.com/read/28412215/involvement-of-serotonergic-noradrenergic-and-dopaminergic-systems-in-the-antidepressant-like-effect-of-ginsenoside-rb1-a-major-active-ingredient-of-panax-ginseng-c-a-meyer
#13
Guo-Li Wang, Zhong-Mei He, Hong-Yan Zhu, Yu-Gang Gao, Yan Zhao, He Yang, Liangxue Zhang
ETHNOPHARMACOLOGY RELEVANCE: Ginsenoside Rb1, a 20 (S)-protopanaxadiol, is a major active ingredient of Panax ginseng C.A. Meyer, which as the King of Chinese herbs, has been wildly used for the treatment of central nervous system diseases. Previous studies have shown that 20 (S)-protopanaxadiol possesses a novel antidepressant-like effect in the treatment of depression, whereas ginsenoside Rb1 in depression has been rarely reported. AIM OF THE REVIEW: The present study was to investigate the antidepressant-like effect of ginsenoside Rb1 and its relevant mechanisms...
April 13, 2017: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/28377625/revealing-the-structure-and-distribution-changes-of-eucalyptus-lignin-during-the-hydrothermal-and-alkaline-pretreatments
#14
Chenzhou Wang, Hanyin Li, Mingfei Li, Jing Bian, Runcang Sun
An integrated pretreatment process based on hydrothermal pretreatment (HTP) followed by alkaline pretreatment has been applied to treat Eucalyptus. The chemical composition and structure changes of lignin during the pretreatment were comprehensively characterized. The surface morphology of the cell walls and lignin distribution of the pretreated Eucalyptus were detected by scanning electron and confocal Raman microscopies. It was found that the chemical bonds between lignin and hemicelluloses were cleaved during the pretreatment...
April 4, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28362293/temperature-induced-first-order-displacive-phase-transition-of-isonicotinamide-4-methoxybenzoic-acid-co-crystal
#15
Tze Shyang Chia, Ching Kheng Quah
Isonicotinamide-4-methoxybenzoic acid co-crystal (1), C6H6N2O·C8H8O3, is formed through slow evaporation from methanol solution and it undergoes a first-order isosymmetry (monoclinic I2/a ↔ monoclinic I2/a) structural phase transition at Tc = 142.5 (5) K, which has been confirmed by an abrupt jump of crystallographic interaxial angle β from variable-temperature single-crystal XRD and small heat hysteresis (6.25 K) in differential scanning calorimetry measurement. The three-dimensional X-ray crystal structures of (1) at the low-temperature phase (LTP) (100, 140 and 142 K) and the high-temperature phase (HTP) (143, 150, 200, 250 and 300 K) were solved and refined as a simple non-disordered model with final R[F(2) > 2σ(F(2))] ≃ 0...
April 1, 2017: Acta Crystallographica Section B, Structural Science, Crystal Engineering and Materials
https://www.readbyqxmd.com/read/28347587/effect-of-hydrothermal-pre-treatment-htp-on-poultry-slaughterhouse-waste-psw-sludge-for-the-enhancement-of-the-solubilization-physical-properties-and-biogas-production-through-anaerobic-digestion
#16
Seyong Park, Young-Man Yoon, Seong Kuk Han, Daegi Kim, Ho Kim
This study is an assessment of the hydrothermal pre-treatment (HTP) of poultry slaughterhouse waste (PSW) sludge for the enhancement of the solubilization, physical properties, and biogas production through anaerobic digestion. This assessment was carried out to ascertain the optimal HTP temperature. The solubilization and physical properties efficacy was investigated by capillary suction time (CST), time to filter (TTF), and particle size. In addition, the anaerobic digestion was investigated through biochemical methane potential (BMP) tests and subsequent statistical analysis using the modified Gompertz model...
March 24, 2017: Waste Management
https://www.readbyqxmd.com/read/28327990/estimating-the-total-number-of-phosphoproteins-and-phosphorylation-sites-in-eukaryotic-proteomes
#17
Panayotis Vlastaridis, Pelagia Kyriakidou, Anargyros Chaliotis, Yves Van de Peer, Stephen G Oliver, Grigoris D Amoutzias
BACKGROUND: Phosphorylation is the most frequent post-translational modification made to proteins and may regulate protein activity as either a molecular digital switch or a rheostat. Despite the cornucopia of high-throughput (HTP) phosphoproteomic data in the last decade, it remains unclear how many proteins are phosphorylated and how many phosphorylation sites (p-sites) can exist in total within a eukaryotic proteome. We present the first reliable estimates of the total number of phosphoproteins and p-sites for four eukaryotes (human, mouse, Arabidopsis, and yeast)...
February 1, 2017: GigaScience
https://www.readbyqxmd.com/read/28327452/sensitive-detection-of-l-5-hydroxytryptophan-based-on-molecularly-imprinted-polymers-with-graphene-amplification
#18
Lu Chen, Hui-Ting Lian, Xiang-Ying Sun, Bin Liu
A novel electrochemical sensor was presented for the determination of L-5-hydroxytryptophan (L-5-HTP) based on a graphene-chitosan molecularly imprinted film modified on the surface of glassy carbon electrode (GR-MIP/GCE). The morphology and composition of the imprinted film were observed in field emission scanning electron microscopy (FESEM), raman spectroscopy and fourier transform infrared (FTIR). The properties of the sensor were evaluated by electrochemical techniques. Under the optimal conditions, the peak currents of L-5-HIP were found to be linear in the concentration range of 0...
June 1, 2017: Analytical Biochemistry
https://www.readbyqxmd.com/read/28295406/lipid-based-submicron-capsules-as-a-strategy-to-include-high-concentrations-of-a-hydrophobic-lightening-agent-in-a-hydrogel
#19
H T P Nguyen, M Soucé, X Perse, F Vial, T Perrier, F Yvergnaux, I Chourpa, E Munnier
OBJECTIVE: This study aimed at increasing the concentration of a hydrophobic lightening agent, Omegalight(®) , in a hydrophilic cosmetic product by means of encapsulation in lipid-based submicron capsules. The core of these capsules is entirely made of the commercial lightening agent. METHODS: Lipid-based encapsulation systems (LNC) were prepared by the PIT method. Their physicochemical properties were followed over 6 months by dynamic light scattering and zeta potential measurements, and in parallel, the potential degradation of the active ingredient was monitored by HPLC...
March 15, 2017: International Journal of Cosmetic Science
https://www.readbyqxmd.com/read/28266211/triphosphate-ion-selective-electrode-based-on-zr-porphyrin-complex
#20
Yuma Yagi, Shuhei Masaki, Tetsuki Iwata, Daisuke Nakane, Takashi Yasui, Akio Yuchi
Ion-selective electrode using zirconium(IV) complex with octaethylporphin (H2oep) as a carrier showed high selectivity to triphosphate (TP, H5tp) against other hydrophilic anions including diphosphate and phosphate. The electroactive species was identified to be [(Zr4(oep)4(Htp)2] (TP/Zr ratio of 0.5) of the unique structure; triphosphates are recognized by one Zr atom through three O atoms on three different P atoms and by another Zr atom through two O atoms on two terminal P atoms and are also involved in complementary intermolecular hydrogen bonding to be surrounded by four porphyrin complexes...
March 16, 2017: Analytical Chemistry
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