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trichosporon asahii

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https://www.readbyqxmd.com/read/27886119/synthesis-and-antifungal-screening-of-2-1-5-alkyl-arylalkylpyrazin-2-yl-ethylidene-hydrazono-1-3-thiazolidin-4-ones
#1
Veronika Opletalova, Jan Dolezel, Jiri Kunes, Vladimir Buchta, Marcela Vejsova, Marta Kucerova-Chlupacova
Two novel thiosemicarbazones and eight novel 2-{[1-(5-alkyl/arylalkylpyrazin-2-yl)ethylidene]hydrazono}-1,3-thiazolidin-4-ones were prepared and tested against a panel of eight fungal strains-Candida albicans ATCC 44859, Candida tropicalis 156, Candida krusei E 28, Candida glabrata 20/I, Trichosporon asahii 1188, Aspergillus fumigatus 231, Lichtheimia corymbifera 272, and Trichophyton interdigitale 445. 1,3-Thiazolidin-4-ones exhibited activity against all strains, the most potent derivative was 2-{[1-(5-butylpyrazin-2-yl)ethylidene]hydrazono}e-1,3-thiazolidin-4-one...
November 23, 2016: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/27862261/cell-surface-hydrophobicity-and-colony-morphology-of-trichosporon-asahii-clinical-isolates
#2
Tomoe Ichikawa, Chihiro Hirata, Mizuki Takei, Naoyuki Tagami, Hiromi Murasawa, Reiko Ikeda
Trichosporon asahii is a pathogenic basidiomycetous yeast. Individual strains of T. asahii have different colony morphologies. However, it is not clear whether cell surface phenotypes differ among the colony morphologies. Here we characterized the cell surface hydrophobicity and analysed the carbohydrate contents of the cell surface polysaccharides in T. asahii clinical isolates with various colony morphologies. Among the three distinctive colony morphologies obtained from one clinical isolate, the white-type morphology exhibited higher hydrophobicity...
November 8, 2016: Yeast
https://www.readbyqxmd.com/read/27816903/molecular-and-antifungal-susceptibility-study-on-trichosporonemia-and-emergence-of-trichosporon-mycotoxinivorans-as-a-bloodstream-pathogen
#3
Yubhisha Dabas, Immaculata Xess, Pratibha Kale
A total of 21 Trichosporon spp. isolates from blood over a period of 5 years (January 2009 to December 2013) were included in the study. The most common underlying diseases found were pancreatitis (33.3%) and cancer (33.3%). Trichosporon asahii (80.9%) was the commonest species followed by Trichosporon mycotoxinivorans (14.2%) and Trichosporon faecale (4.7%). On IGS1 region sequencing the most predominant T. asahii type in our region was genotype 1 (16/17 isolates; 94.1%) and one isolate belonged to genotype 4...
November 5, 2016: Medical Mycology: Official Publication of the International Society for Human and Animal Mycology
https://www.readbyqxmd.com/read/27799926/invasive-trichosporon-infection-a-systematic-review-on-a-re-emerging-fungal-pathogen
#4
REVIEW
João N de Almeida Júnior, Christophe Hennequin
Objectives: This review aimed to better depict the clinical features and address the issue of therapeutic management of Trichosporon deep-seated infections. Methods: We comprehensively reviewed the cases of invasive Trichosporon infection reported in the literature from 1994 (date of taxonomic modification) to 2015. Data from antifungal susceptibility testing (AST) studies were also analyzed. Results: Two hundred and three cases were retained and split into four groups: homeopathy (n = 79), other immunodeficiency conditions (n = 41), miscellaneous (n = 58) and newborns (n = 25)...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27794247/diversity-of-spoilage-fungi-associated-with-various-french-dairy-products
#5
Lucille Garnier, Florence Valence, Audrey Pawtowski, Lizaveta Auhustsinava-Galerne, Nicolas Frotté, Riccardo Baroncelli, Franck Deniel, Emmanuel Coton, Jérôme Mounier
Yeasts and molds are responsible for dairy product spoilage, resulting in significant food waste and economic losses. Yet, few studies have investigated the diversity of spoilage fungi encountered in dairy products. In the present study, 175 isolates corresponding to 105 from various spoiled dairy products and 70 originating from dairy production environments, were identified using sequencing of the ITS region, the partial β-tubulin, calmodulin and/or EFα genes, and the D1-D2 domain of the 26S rRNA gene for filamentous fungi and yeasts, respectively...
October 21, 2016: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/27784941/study-on-antioxidant-enzymatic-activities-of-trichosporon-asahii
#6
Yangmei Zhang, Haitao Li, Rongya Yang, Congmin Wang
Superoxide dismutase (SOD) and catalase are considered the most important antioxidant enzymes which protect fungus from the oxidant damage of reactive oxygen species. In this study, we collected 44 strains of Trichosporon asahii (T. asahii) from different sources and investigated their SOD and catalase activities. The results showed that the SOD and catalase activities of Clinical group were significantly higher than those of Environment group (p < 0.01). The SOD and catalase activities of T. asahii in Internal passage group went up gradually after passage in mice, and were significantly higher in 5th generation of Internal passage group (p < 0...
December 2016: Indian Journal of Microbiology
https://www.readbyqxmd.com/read/27697432/antifungal-activities-of-tacrolimus-in-combination-with-antifungal-agents-against-fluconazole-susceptible-and-fluconazole-resistant-trichosporon-asahii-isolates
#7
Thaís Felli Kubiça, Laura Bedin Denardi, Maria Isabel Azevedo, Vanessa Oliveira, Luiz Carlos Severo, Janio Morais Santurio, Sydney Hartz Alves
The antifungal activity of tacrolimus in combination with antifungal agents against different fungal species has been previously reported. Here we report the in vitro interactions between tacrolimus and amphotericin B, fluconazole, itraconazole, and caspofungin against 30 clinical isolates of both fluconazole-susceptible and fluconazole-resistant Trichosporon asahii. For these analyses, we used the broth microdilution method based on the M27-A3 technique and checkerboard microdilution method. Tacrolimus showed no activity against T...
September 30, 2016: Brazilian Journal of Infectious Diseases
https://www.readbyqxmd.com/read/27495321/fungemia-due-to-rare-opportunistic-yeasts-data-from-a-population-based-surveillance-in-spain
#8
Mario Fernández-Ruiz, Jesús Guinea, Mireia Puig-Asensio, Óscar Zaragoza, Benito Almirante, Manuel Cuenca-Estrella, José María Aguado
Fungemia due to rare yeasts constitutes an emerging but poorly investigated condition. Data on risk factors, clinical features, therapy, and outcome of episodes of fungemia due to rare (non-Candida, non-Cryptococcus) yeasts were analyzed in a population-based surveillance program conducted in 29 Spanish hospitals between May 2010 and April 2011. Species identification (DNA sequencing) and antifungal susceptibility testing (EUCAST and CLSI methods) were centrally performed. Fourteen out of 767 episodes of fungemia (1...
August 4, 2016: Medical Mycology: Official Publication of the International Society for Human and Animal Mycology
https://www.readbyqxmd.com/read/27494105/production-of-bioactive-protein-hydrolysate-using-the-yeasts-isolated-from-soft-chhurpi
#9
Amit Kumar Rai, Reena Kumari, Samurailatpam Sanjukta, Dinabandhu Sahoo
The aim of this work was to study the production of bioactive protein hydrolysates using yeasts isolated from chhurpi. For this, a total of 125 proteolytic yeasts were isolated and molecular identification was carried out by analysis of the restriction digestion pattern generated by digesting the PCR amplified internal transcribed spacer region and 5.8S rRNA gene (ITS1-5.8S-ITS2) using three endonucleases (HaeIII, CfoI and HinfI). The results obtained showed that different proteolytic yeasts were dominant in marketed products (Kluyveromyces marxianus and Issatchenkia orientalis) and samples from production centers (Trichosporon asahii, Saccharomyces cerevisiae and Exophiala dermatitidis)...
November 2016: Bioresource Technology
https://www.readbyqxmd.com/read/27317582/rapid-identification-of-moulds-and-arthroconidial-yeasts-from-positive-blood-cultures-by-maldi-tof-mass-spectrometry
#10
João N de Almeida, Jaques Sztajnbok, Afonso Rafael da Silva, Vinicius Adriano Vieira, Anne Layze Galastri, Leandro Bissoli, Nadia Litvinov, Gilda Maria Barbaro Del Negro, Adriana Lopes Motta, Flávia Rossi, Gil Benard
Moulds and arthroconidial yeasts are potential life-threatening agents of fungemia in immunocompromised patients. Fast and accurate identification (ID) of these pathogens hastens initiation of targeted antifungal therapy, thereby improving the patients' prognosis. We describe a new strategy that enabled the identification of moulds and arthroconidial yeasts directly from positive blood cultures by MALDI-TOF mass spectrometry (MS). Positive blood cultures (BCs) with Gram staining showing hyphae and/or arthroconidia were prospectively selected and submitted to an in-house protein extraction protocol...
November 1, 2016: Medical Mycology: Official Publication of the International Society for Human and Animal Mycology
https://www.readbyqxmd.com/read/27275608/in-vitro-interactions-between-non-steroidal-anti-inflammatory-drugs-and-antifungal-agents-against-planktonic-and-biofilm-forms-of-trichosporon-asahii
#11
Suteng Yang, Yong Liao, Lin Cong, Xuelian Lu, Rongya Yang
Increasing drug resistance has brought enormous challenges to the management of Trichosporon spp. infections. The in vitro antifungal activities of non-steroidal anti-inflammatory drugs (NSAIDs) against Candida spp. and Cryptococcus spp. were recently discovered. In the present study, the in vitro interactions between three NSAIDs (aspirin, ibuprofen and diclofenac sodium) and commonly used antifungal agents (fluconazole, itraconazole, voriconazole, caspofungin and amphotericin B) against planktonic and biofilm cells of T...
2016: PloS One
https://www.readbyqxmd.com/read/27144725/molecular-characterisation-and-antifungal-susceptibility-of-clinical-trichosporon-isolates-in-india
#12
Vijaylatha Rastogi, Prasanna Honnavar, Shivaprakash M Rudramurthy, Umabala Pamidi, Anup Ghosh, Arunaloke Chakrabarti
In Asian countries, Trichosporon infection is a well-known disease in Japan. In India, the infection is increasingly recognised. The study was conducted to characterise the clinical Trichosporon isolates from India by phenotypic and molecular techniques. A total of 31 Trichosporon clinical isolates, recovered from patients of 14 hospitals across India were sequenced (ITS and IGS1 regions of rDNA). In vitro drug susceptibility testing of the isolates was performed against amphotericin-B, fluconazole, itraconazole, voriconazole and posaconazole...
August 2016: Mycoses
https://www.readbyqxmd.com/read/27105313/sequencer-based-capillary-gel-electrophoresis-scge-targeting-the-rdna-internal-transcribed-spacer-its-regions-for-accurate-identification-of-clinically-important-yeast-species
#13
Xin Hou, Meng Xiao, Sharon C-A Chen, He Wang, Li Zhang, Xin Fan, Zhi-Peng Xu, Jing-Wei Cheng, Fanrong Kong, Yu-Pei Zhao, Ying-Chun Xu
Accurate species identification of Candida, Cryptococcus, Trichosporon and other yeast pathogens is important for clinical management. In the present study, we developed and evaluated a yeast species identification scheme by determining the rDNA internal transcribed spacer (ITS) region length types (LTs) using a sequencer-based capillary gel electrophoresis (SCGE) approach. A total of 156 yeast isolates encompassing 32 species were first used to establish a reference SCGE ITS LT database. Evaluation of the ITS LT database was then performed on (i) a separate set of (n = 97) clinical isolates by SCGE, and (ii) 41 isolates of 41 additional yeast species from GenBank by in silico analysis...
2016: PloS One
https://www.readbyqxmd.com/read/27048799/deacetylation-of-fungal-exopolysaccharide-mediates-adhesion-and-biofilm-formation
#14
Mark J Lee, Alexander M Geller, Natalie C Bamford, Hong Liu, Fabrice N Gravelat, Brendan D Snarr, François Le Mauff, Joseé Chabot, Benjamin Ralph, Hanna Ostapska, Mélanie Lehoux, Robert P Cerone, Stephanie D Baptista, Evgeny Vinogradov, Jason E Stajich, Scott G Filler, P Lynne Howell, Donald C Sheppard
UNLABELLED: The mold Aspergillus fumigatus causes invasive infection in immunocompromised patients. Recently, galactosaminogalactan (GAG), an exopolysaccharide composed of galactose and N-acetylgalactosamine (GalNAc), was identified as a virulence factor required for biofilm formation. The molecular mechanisms underlying GAG biosynthesis and GAG-mediated biofilm formation were unknown. We identified a cluster of five coregulated genes that were dysregulated in GAG-deficient mutants and whose gene products share functional similarity with proteins that mediate the synthesis of the bacterial biofilm exopolysaccharide poly-(β1-6)-N-acetyl-D-glucosamine (PNAG)...
2016: MBio
https://www.readbyqxmd.com/read/26930469/selective-disruption-of-disulphide-bonds-lowered-activation-energy-and-improved-catalytic-efficiency-in-talipb-from-trichosporon-asahii-msr54-md-simulations-revealed-flexible-lid-and-extended-substrate-binding-area-in-the-mutant
#15
Yogesh Singh, Namita Gupta, Ved Vrat Verma, Manisha Goel, Rani Gupta
TALipB (33 kDa) is a solvent stable, enantioselective lipase from Trichosporon asahii MSR54. It is cysteine-rich and shows activation in presence of thiol reducing agents. DIANNA server predicted three disulphide bridges C53-C195 (S1), C89-C228 (S2) and C164-C254 (S3) in the enzyme. Selective disruption of disulphide bonds by cysteine to alanine mutations at Cys53 and Cys89 of S1 and S2 bonds resulted in enzyme activation. Mutant mTALipB (S1+S2) showed increase in specific activity by ∼4-fold (834 mM/mg) and improved Vmax of 6...
March 25, 2016: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/26916812/genotype-antifungal-susceptibility-and-biofilm-formation-of-trichosporon-asahii-isolated-from-the-urine-of-hospitalized-patients
#16
Adriana Araújo de Almeida, Bruno do Amaral Crispim, Alexéia Barufatti Grisolia, Terezinha Inez Estivalet Svidzinski, Lais Gonçalvez Ortolani, Kelly Mari Pires de Oliveira
In this study, the culture analysis of urine samples from patients hospitalized in the Central-West region of Brazil was performed, and the isolated microorganisms were phylogenetically identified as Trichosporon asahii. Maximum parsimony analysis of the IGS1 sequences revealed three novel genotypes that have not been described. The minimum inhibitory concentrations of the nine isolates identified were in the range of 0.06-1 μg/ml for amphotericin B, 0.25-4 μg/ml for fluconazole, and 0.03-0.06 μg/ml for itraconazole...
January 2016: Revista Argentina de Microbiología
https://www.readbyqxmd.com/read/26887254/the-dynamic-structure-of-spitzenk%C3%A3-rpers-of-trichosporon-asahii-examined-by-the-fluorescent-probe-fm4-64
#17
Yuntong Lv, Zhikuan Xia, Wenling Wang
The Spitzenkörper is a dynamic and specialized multicomponent cell complex present in the tips of hyphal cells. The amphiphilic styryl dye FM4-64 was found to be ideal for imaging the dynamic changes of the apical vesicle cluster within growing hyphal tips. It is widely used as a marker of endocytosis and to visualize vacuolar membranes. Here we performed uptake experiments using FM4-64 to study the dynamic of the Spitzenkörper in Trichosporon asahii. We observed that Spitzenkörpers were present at the tip of the budding site of the spore, blastospore, and the germ tube of T...
January 2016: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/26887224/azole-resistance-in-candida-spp-isolated-from-cat%C3%A3%C2%BA-lake-cear%C3%A3-brazil-an-efflux-pump-mediated-mechanism
#18
Raimunda S N Brilhante, Manoel A N Paiva, Célia M S Sampaio, Débora S C M Castelo-Branco, Carlos E C Teixeira, Lucas P de Alencar, Tereza J P G Bandeira, André J Monteiro, Rossana A Cordeiro, Waldemiro A Pereira-Neto, José J C Sidrim, José L B Moreira, Marcos F G Rocha
Since, there is no study reporting the mechanism of azole resistance among yeasts isolated from aquatic environments; the present study aims to investigate the occurrence of antifungal resistance among yeasts isolated from an aquatic environment, and assess the efflux-pump activity of the azole-resistant strains to better understand the mechanism of resistance for this group of drugs. For this purpose, monthly water and sediment samples were collected from Catú Lake, Ceará, Brazil, from March 2011 to February 2012...
January 2016: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/26787003/occurrence-of-mycotoxins-and-yeasts-and-moulds-identification-in-corn-silages-in-tropical-climate
#19
B F Carvalho, C L S Ávila, P M Krempser, L R Batista, M N Pereira, R F Schwan
AIMS: This study was aimed to identify yeasts and moulds as well as to detect mycotoxin in corn silages in southern Minas Gerais, Brazil. METHODS AND RESULTS: Corn silages from 36 farms were sampled to analyse dry matter, crude protein, ether extract, ash, neutral detergent fibre, nonfibre carbohydrates and mycotoxins contents, yeasts and moulds population, pH and temperature values. The mycotoxins found in high frequency were aflatoxin in 77·7% of analysed samples, ochratoxin (33·3%) and zearalenone (22·2%)...
May 2016: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/26777248/novel-s-enantioselective-lipase-talipb-from-trichosporon-asahii-msr54-heterologous-expression-characterization-conformational-stability-and-homology-modeling
#20
Yogesh Singh, Rani Gupta
A novel lipase encoding gene, TALipB from Trichosporon asahii MSR54 was heterologously expressed in Escherichia coli using three vectors, pET22b, pET28a & pEZZ18. The three recombinant proteins, viz. C-hexahistidine fused HLipB, N and C-hexahistidine fused HLipBH and ZZ-fused ZZLipB were purified using affinity chromatography. All the three enzymes were mid to long fatty acyl chain selective on p-NP esters and S-enantioselective irrespective of tags. HLipB had lowest activation energy (3.5 Kcal mol(-1)) and highest catalytic efficiency (254 mM(-1) min(-1)) on p-NP caprate followed by HLipBH and ZZLipB...
February 2016: Enzyme and Microbial Technology
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