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https://www.readbyqxmd.com/read/28729092/annexin-a2-modulated-proliferation-of-pulmonary-arterial-smooth-muscle-cells-depends-on-caveolae-and-caveolin-1-in-hepatopulmonary-syndrome
#1
Lin Liao, Binwu Zheng, Bin Yi, Chang Liu, Lin Chen, Ziyang Zeng, Jing Gao
We have established that annexin A2 (ANXA2) is an important factor in the experimental hepatopulmonary syndrome (HPS) serum-induced proliferation of pulmonary arterial smooth muscle cells (PASMCs). However, the detailed mechanism remains unclear. ANXA2 translocated to the caveolin-enriched microdomains (caveolae) in PASMCs upon HPS serum stimulation. The disruption of caveolae by Methyl-β-cyclodextrin (MβCD) alleviated the caveolae recruitment of ANXA2 and the ANXA2-mediated activation of ERK1/2 and NF-κB, so that ANXA2-modulated PASMC proliferation was suppressed...
July 17, 2017: Experimental Cell Research
https://www.readbyqxmd.com/read/28726952/porphyra-polysaccharide-derived-carbon-dots-for-non-viral-co-delivery-of-different-gene-combinations-and-neuronal-differentiation-of-ectodermal-mesenchymal-stem-cells
#2
Jiaxin Chen, Qiang Wang, Jie Zhou, Wenwen Deng, Qingtong Yu, Xia Cao, Jianping Wang, Fengxia Shao, Yang Li, Ping Ma, Myron Spector, Jiangnan Yu, Ximing Xu
In this study, multifunctional fluorescent carbon dots (CDs) were synthesized using a one-pot hydrothermal carbonization reaction, with the naturally-occurring porphyra polysaccharide (PPS) serving as a single carbon source for the first time and ethylenediamine (Ed) acting as the surface passivation agent. The resulting CDs enjoyed a high quantum yield (56.3%), excitation-dependent fluorescence, small size (<10 nm), spherical shape, uniform distribution, positive surface charge, low cytotoxicity and excellent ability to condense macromolecular plasmid DNA...
July 20, 2017: Nanoscale
https://www.readbyqxmd.com/read/28725180/endothelial-nitric-oxide-synthase-is-present-in-dendritic-spines-of-neurons-in-primary-cultures
#3
Ariel Caviedes, Manuel Varas-Godoy, Carlos Lafourcade, Soledad Sandoval, Javiera Bravo-Alegria, Thilo Kaehne, Angela Massmann, Jorge P Figueroa, Francisco Nualart, Ursula Wyneken
Nitric oxide exerts important regulatory functions in various brain processes. Its synthesis in neurons has been most commonly ascribed to the neuronal nitric oxide synthase (nNOS) isoform. However, the endothelial isoform (eNOS), which is significantly associated with caveolae in different cell types, has been implicated in synaptic plasticity and is enriched in the dendrites of CA1 hippocampal neurons. Using high resolution microscopy and co-distribution analysis of eNOS with synaptic and raft proteins, we now show for the first time in primary cortical and hippocampal neuronal cultures, virtually devoid of endothelial cells, that eNOS is present in neurons and is localized in dendritic spines...
2017: Frontiers in Cellular Neuroscience
https://www.readbyqxmd.com/read/28724764/rab5-and-rab11-are-required-for-clathrin-dependent-endocytosis-of-japanese-encephalitis-virus-in-bhk-21-cells
#4
Chun-Chun Liu, Yun-Na Zhang, Zhao-Yao Li, Jin-Xiu Hou, Jing Zhou, Lin Kan, Bin Zhou, Pu-Yan Chen
During infection Japanese Encephalitis Virus generally enters host cells via receptor-mediated clathrin dependent endocytosis. The trafficking of JEV within endosomes is controlled by Rab GTPases, but which Rab proteins are involved in JEV entry into BHK-21 cells is unknown. In this study, entry and post-internalization of JEV were analyzed using biochemical inhibitors, RNA interference and dominant negative mutants. Our data demonstrate that JEV entry into BHK-21 cells depends on clathrin, dynamin, and cholesterol, but not caveolae or macropinocytosis...
July 19, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28700237/rgd-modified-albumin-nanoconjugates-for-targeted-delivery-of-a-porphyrin-photosensitizer
#5
Fang Li, Yan Zhao, Chengqiong Mao, Yi Kong, Xin Ming
Advances in photodynamic therapy of cancer have been restrained by lack of cancer specificity and side effects to normal tissues. Molecularly targeted photodynamic therapy can achieve higher cancer specificity by combination of active cancer targeting and localized laser activation. We aimed to use albumin as a carrier to prepare targeted nanoconjugates that are selective to cancer cells and smaller than conventional nanoparticles for superior tumor penetration. IRDye® 700DX (IR700), a porphyrin photosensitizer, was covalently conjugated to human serum albumin that was also linked with tumor-targeting RGD peptides...
July 12, 2017: Molecular Pharmaceutics
https://www.readbyqxmd.com/read/28698382/phosphatidylserine-dictates-the-assembly-and-dynamics-of-caveolae-in-the-plasma-membrane
#6
Takashi Hirama, Raibatak Das, Yanbo Yang, Charles Ferguson, Amy Won, Christopher M Yip, Jason G Kay, Sergio Grinstein, Robert G Parton, Gregory D Fairn
Caveolae are bulb-shaped nanodomains of the plasma membrane that are enriched in cholesterol and sphingolipids. They have many physiological functions, including endocytic transport, mechanosensing, and regulation of membrane and lipid transport. Caveola formation relies on integral membrane proteins termed caveolins (CAVs) and the cavin family of peripheral proteins. Both protein families bind anionic phospholipids, but the precise roles of these lipids are unknown. Here, we studied the effects of phosphatidylserine (PtdSer), phosphatidylinositol 4-phosphate (PtdIns4P), and phosphatidylinositol 4,5-bisphosphate (PtdIns4,5P2) on caveolar formation and dynamics...
July 11, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28696684/development-of-beta-carotene-loaded-organogel-based-nanoemulsion-with-improved-in-vitro-and-in-vivo-bioaccessibility
#7
Yuting Fan, Luyu Gao, Jiang Yi, Yuzhu Zhang, Wallace Yokoyama
Beta-carotene (BC), a naturally occurring lipophilic carotenoid, is beneficial for human health. However, its water solubility and bioavailability is low. In this study, organogel-based nanoemulsion was successfully prepared to improve BC's loading amount, solubility, and bioavailability. Corn oil was selected as oil phase for organogel due to the greatest release amount of BC. Tween 20 was optimized as the emulsifier based on the highest extent of lipolysis and BC bioaccessibility. The nanoemulsion was a better alternative than organogel according to both the extent of lipolysis and BC bioaccessibility...
July 11, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28686970/endocytic-pathways-of-optimized-resveratrol-cubosomes-capturing-into-human-hepatoma-cells
#8
Hend Mohamed Abdel-Bar, Rania Abd El Basset Sanad
Resveratrol (RSV) is a natural polyphenolic compound with high affinity to hepatocytes. It has numerous benefits as anticancer, antioxidant, immunomodulatory and cardioprotective actions. Nevertheless, RSV therapeutic applications are hindered by its low solubility, light sensitivity and extensive first-pass metabolism. Cubosomes are colloidally stable dispersed liquid crystalline nanoparticles. The incorporation of RSV into cubosomes could overcome some of its physicochemical limitations. A Design-Expert(®) software was applied to optimize cubosomes in terms of particle size and encapsulation efficiency (EE%)...
July 4, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28662517/caveolae-depletion-contributes-to-vasorelaxant-effects-of-chenodeoxycholic-acid
#9
Zhongchao Wang, Qiang Lv, Huan Liu, Yue Wu, Yungang Bai, Yaoping Cheng, Yuting Su, Yue Cai, Jinwen Yu, Jin Ma, Junxiang Bao
BACKGROUND/AIMS: High concentration of bile acids (BAs) induces hydrophobicity-dependent vasorelaxtant effects with hydrophobic BAs showing greater responses than hydrophilic BAs, of which the underlying mechanisms are still unclear. Caveolae are invaginations on membranes of endothelial cells (ECs) entraping endothelial nitric oxide synthase (eNOS) to prevent its activation, which plays a critical role in regulation of vascular function. The purpose of the present study was to investigate the role of caveolae in vasorelaxant effects of BAs...
June 28, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28656597/adherens-junctions-and-desmosomes-are-damaged-by-entamoeba-histolytica-participation-of-ehcpadh-complex-and-ehcp112-protease
#10
Elizabeth Hernández-Nava, Patricia Cuellar, Porfirio Nava, Bibiana Chávez-Munguía, Michael Schnoor, Esther Orozco, Abigail Betanzos
Entamoeba histolytica trophozoites adhere to epithelium at the cell-cell contact and perturb tight junctions (TJs) disturbing the transepithelial electrical resistance. Behind TJs are the adherens junctions (AJs) that reinforce them; and the desmosomes (DSMs) that maintain the epithelium integrity. The damage produced to AJs and DMSs by this parasite is unknown. Here, we studied the effect of the trophozoites, the EhCPADH complex and the EhCP112 recombinant enzyme (rEhCP112) on AJ and DSM proteins. We found that trophozoites degraded β-cat, E-cad, Dsp l/ll and Dsg-2 with the participation of EhCPADH and EhCP112...
June 27, 2017: Cellular Microbiology
https://www.readbyqxmd.com/read/28652618/intracellular-targeting-of-annexin-a2-inhibits-tumor-cell-adhesion-migration-and-in-vivo-grafting
#11
Daniela I Staquicini, Roberto Rangel, Liliana Guzman-Rojas, Fernanda I Staquicini, Andrey S Dobroff, Christy A Tarleton, Michelle A Ozbun, Mikhail G Kolonin, Juri G Gelovani, Serena Marchiò, Richard L Sidman, Katherine A Hajjar, Wadih Arap, Renata Pasqualini
Cytoskeletal-associated proteins play an active role in coordinating the adhesion and migration machinery in cancer progression. To identify functional protein networks and potential inhibitors, we screened an internalizing phage (iPhage) display library in tumor cells, and selected LGRFYAASG as a cytosol-targeting peptide. By affinity purification and mass spectrometry, intracellular annexin A2 was identified as the corresponding binding protein. Consistently, annexin A2 and a cell-internalizing, penetratin-fused version of the selected peptide (LGRFYAASG-pen) co-localized and specifically accumulated in the cytoplasm at the cell edges and cell-cell contacts...
June 26, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28651541/the-agglomeration-state-of-nanoparticles-can-influence-the-mechanism-of-their-cellular-internalisation
#12
Blanka Halamoda-Kenzaoui, Mara Ceridono, Patricia Urbán, Alessia Bogni, Jessica Ponti, Sabrina Gioria, Agnieszka Kinsner-Ovaskainen
BACKGROUND: Significant progress of nanotechnology, including in particular biomedical and pharmaceutical applications, has resulted in a high number of studies describing the biological effects of nanomaterials. Moreover, a determination of so-called "critical quality attributes", that is specific physicochemical properties of nanomaterials triggering the observed biological response, has been recognised as crucial for the evaluation and design of novel safe and efficacious therapeutics...
June 26, 2017: Journal of Nanobiotechnology
https://www.readbyqxmd.com/read/28648824/sheath-cell-invasion-and-trans-differentiation-repair-mechanical-damage-caused-by-loss-of-caveolae-in-the-zebrafish-notochord
#13
Jamie Garcia, Jennifer Bagwell, Brian Njaine, James Norman, Daniel S Levic, Susan Wopat, Sara E Miller, Xiaojing Liu, Jason W Locasale, Didier Y R Stainier, Michel Bagnat
The notochord, a conserved axial structure required for embryonic axis elongation and spine development, consists of giant vacuolated cells surrounded by an epithelial sheath [1-3]. During morphogenesis, vacuolated cells maintain their structural integrity despite being under constant mechanical stress [4]. We hypothesized that the high density of caveolae present in vacuolated cells [5, 6] could buffer mechanical tension. Caveolae are 50- to 80-nm membrane invaginations lined by cage-like polygonal structures [7, 8] formed by caveolin 1 (Cav1) or Cav3 and one of the cavin proteins [6, 9-11]...
July 10, 2017: Current Biology: CB
https://www.readbyqxmd.com/read/28648821/caveolae-protect-notochord-cells-against-catastrophic-mechanical-failure-during-development
#14
Ye-Wheen Lim, Harriet P Lo, Charles Ferguson, Nick Martel, Jean Giacomotto, Guillermo A Gomez, Alpha S Yap, Thomas E Hall, Robert G Parton
The embryonic notochord is a flexible structure present during development that serves as scaffold for formation of the vertebrate spine. This rod-like organ is thought to have evolved in non-vertebrate chordates to facilitate locomotion by providing a rigid but flexible midline structure against which the axial muscles can contract. This hydrostatic "skeleton" is exposed to a variety of mechanical forces during oscillation of the body. There is evidence that caveolae, submicroscopic cup-shaped plasma membrane pits, can buffer tension in cells that undergo high levels of mechanical stress...
July 10, 2017: Current Biology: CB
https://www.readbyqxmd.com/read/28648645/functional-interaction-of-the-two-pore-domain-potassium-channel-task-1-and-caveolin-3
#15
Chen Kang, Victor A Hernandez, Keli Hu
The two-pore domain potassium channel TASK-1 is strongly expressed in the heart and has been shown to modulate the resting membrane potential and action potential. However, little is known about the regulation of TASK-1 channels. The present study was designed to determine whether TASK-1 is modulated by caveolin-3, a primary structural protein of cardiac caveolae. Functional studies with the whole-cell voltage clamp technique showed that the expression of caveolin-3 decreased recombinant TASK-1 currents significantly in HEK293T cells, and this effect was prevented by co-expressing the dominant negative mutant caveolin-3 P104L...
June 23, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28641181/the-caveolae-dress-code-structure-and-signaling
#16
REVIEW
Christophe Lamaze, Nicolas Tardif, Melissa Dewulf, Stéphane Vassilopoulos, Cédric M Blouin
Over the past decade, interest in caveolae biology has peaked. These small bulb-shaped plasma membrane invaginations of 50-80nm diameter present in most cell types have been upgraded from simple membrane structures to a more complex bona fide organelle. However, although caveolae are involved in several essential cellular functions and pathologies, the underlying molecular mechanisms remain poorly defined. Following the identification of caveolins and cavins as the main caveolae constituents, recent studies have brought new insight into their structural organization as a coat...
June 19, 2017: Current Opinion in Cell Biology
https://www.readbyqxmd.com/read/28637676/editorial-focus-on-balance-between-s-nitrosylation-and-denitrosylation-modulates-myoblast-proliferation-independently-of-soluble-guanylyl-cyclase-activation
#17
Hugo P Monteiro, Fernando T Ogata
Myogenesis involves a complex series of signaling events that will result in the formation of muscle fibers. The participation of nitric oxide (NO) in myogenesis is well established. NO generation in skeletal muscle comes from the neuronal isoform of the NO synthase (nNOS). NO signals through cGMP production and s-nitrosylation of proteins in skeletal muscle. In skeletal muscle, nNOS interacts with two domains of caveolin-3 which is part of caveolae, and forms a signaling complex through additional interactions with Src kinase and p21Ras...
June 21, 2017: American Journal of Physiology. Cell Physiology
https://www.readbyqxmd.com/read/28604134/impact-of-exposure-time-particle-size-and-uptake-pathway-on-silver-nanoparticle-effects-on-circulating-immune-cells-in-mytilus-galloprovincialis
#18
Younes Bouallegui, Ridha Ben Younes, Faten Turki, Ridha Oueslati
Nanomaterials have increasingly emerged as potential pollutants to aquatic organisms. Nanomaterials are known to be taken up by hemocytes of marine invertebrates including Mytilus galloprovincialis. Indeed, assessments of hemocyte-related parameters are a valuable tool in the determination of potentials for nanoparticle (NP) toxicity. The present study assessed the effects from two size types of silver nanoparticles (AgNP: <50 nm and <100 nm) on the frequency of hemocytes subpopulations as immunomodulation biomarkers exposed in a mollusk host...
December 2017: Journal of Immunotoxicology
https://www.readbyqxmd.com/read/28593998/the-ubiquitin-ligase-znrf1-promotes-caveolin-1-ubiquitination-and-degradation-to-modulate-inflammation
#19
Chih-Yuan Lee, Ting-Yu Lai, Meng-Kun Tsai, Yung-Chi Chang, Yu-Hsin Ho, I-Shing Yu, Tzu-Wen Yeh, Chih-Chang Chou, You-Sheng Lin, Toby Lawrence, Li-Chung Hsu
Caveolin-1 (CAV1), the major constituent of caveolae, plays a pivotal role in various cellular biological functions, including cancer and inflammation. The ubiquitin/proteasomal pathway is known to contribute to the regulation of CAV1 expression, but the ubiquitin ligase responsible for CAV1 protein stability remains unidentified. Here we reveal that E3 ubiquitin ligase ZNRF1 modulates CAV1 protein stability to regulate Toll-like receptor (TLR) 4-triggered immune responses. We demonstrate that ZNRF1 physically interacts with CAV1 in response to lipopolysaccharide and mediates ubiquitination and degradation of CAV1...
June 8, 2017: Nature Communications
https://www.readbyqxmd.com/read/28587148/the-role-of-caveolin-1-in-hiv-infection-and-pathogenesis
#20
REVIEW
Ayalew Mergia
Caveolin 1 (Cav-1) is a major component of the caveolae structure and is expressed in a variety of cell types including macrophages, which are susceptible to human immunodeficiency virus (HIV) infection. Caveolae structures are present in abundance in mechanically stressed cells such as endothelial cells and adipocytes. HIV infection induces dysfunction of these cells and promotes pathogenesis. Cav-1 and the caveolae structure are believed to be involved in multiple cellular processes that include signal transduction, lipid regulation, endocytosis, transcytosis, and mechanoprotection...
May 26, 2017: Viruses
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