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https://www.readbyqxmd.com/read/27894940/changes-in-the-mrna-expression-of-structural-proteins-hormone-synthesis-and-secretion-from-bovine-placentome-sections-after-ddt-and-dde-treatment
#1
A Wojciechowska, J Mlynarczuk, J Kotwica
Disorders in the barrier function and secretory activity of the placenta can be caused by xenobiotics (XB) present in the environment and their accumulation in tissues of living organisms. Thus, the aim of this study was to investigate the effect of 1,1,1-trichloro-2,2,-bis-4-chlorophenyl-ethane (DDT) and its metabolite 1,1-dichloro-2,2-bis-4-chlorophenyl-ethene (DDE) (for 24 or 48h) at doses of 1, 10 or 100ng/ml on the function of cow placentome sections in the second trimester of pregnancy. DDT and DDE affected neither (P>0...
November 25, 2016: Toxicology
https://www.readbyqxmd.com/read/27824861/identification-of-biomarkers-of-impaired-sensory-profiles-among-autistic-patients
#2
Afaf El-Ansary, Wail M Hassan, Hanan Qasem, Undurti N Das
BACKGROUND: Autism is a neurodevelopmental disorder that displays significant heterogeneity. Comparison of subgroups within autism, and analyses of selected biomarkers as measure of the variation of the severity of autistic features such as cognitive dysfunction, social interaction impairment, and sensory abnormalities might help in understanding the pathophysiology of autism. METHODS AND PARTICIPANTS: In this study, two sets of biomarkers were selected. The first included 7, while the second included 6 biomarkers...
2016: PloS One
https://www.readbyqxmd.com/read/27816460/cuprac-colorimetric-and-electroanalytical-methods-determining-antioxidant-activity-based-on-prevention-of-oxidative-dna-damage
#3
Seda Uzunboy, Sema Demirci Çekiç, Ece Eksin, Arzum Erdem, Reşat Apak
An unbalanced excess of oxygen/nitrogen species (ROS/RNS) can give oxidative hazard to DNA and other biomacromolecules under oxidative stress conditions. While the 'comet' assay for measuring DNA damage is neither specific nor practical, monitoring oxidative changes on individual DNA bases and other oxidation products needs highly specialized equipment and operators. Thus, we developed a modified CUPRAC (cupric ion reducing antioxidant capacity) colorimetric method to determine the average total damage on DNA produced by Fenton oxidation, taking advantage of the fact that the degradation products of DNA but not the original macromolecule is CUPRAC-responsive...
November 2, 2016: Analytical Biochemistry
https://www.readbyqxmd.com/read/27733774/time-lagging-interplay-effect-and-excess-risk-of-meteorological-mosquito-parameters-and-petrochemical-gas-explosion-on-dengue-incidence
#4
Ko Chang, Chaur-Dong Chen, Chien-Ming Shih, Tzu-Chi Lee, Ming-Tsang Wu, Deng-Chyang Wu, Yen-Hsu Chen, Chih-Hsing Hung, Meng-Chieh Wu, Chun-Chi Huang, Chien-Hung Lee, Chi-Kung Ho
In Kaohsiung, a metropolitan city in Taiwan at high risk of dengue epidemic, weather factors combined with an accidental petrochemical gas explosion (PGE) may affect mosquito‒human dynamics in 2014. Generalized estimating equations with lagged-time Poisson regression analyses were used to evaluate the effect of meteorological/mosquito parameters and PGE on dengue incidences (2000-2014) in Kaohsiung. Increased minimum temperatures rendered a 2- and 3-month lagging interactive effect on higher dengue risks, and higher rainfall exhibited a 1- and 2-month lagging interplay effect on lower risks (interaction, P ≤ 0...
October 13, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27563731/effects-of-tio2-and-tic-nanofillers-on-the-performance-of-dye-sensitized-solar-cells-based-on-the-polymer-gel-electrolyte-of-a-cobalt-redox-system
#5
Shanmuganathan Venkatesan, I-Ping Liu, Li-Tung Chen, Yi-Chen Hou, Chiao-Wei Li, Yuh-Lang Lee
Polymer gel electrolytes (PGEs) of cobalt redox system are prepared for dye sensitized solar cell (DSSC) applications. Poly(vinylidene fluoride-co-hexafluoropropylene) (PVDF-HFP) is used as a gelator of an acetonitrile (ACN) liquid electrolyte containing tris(2,2'-bipyridine)cobalt(II/III) redox couple. Titanium dioxide (TiO2) and titanium carbide (TiC) nanoparticles are utilized as nanofillers (NFs) of this PGE, and the effects of the two NFs on the conductivity of the PGEs, charge-transfer resistances at the electrode/PGE interface, and the performance of the gel-state DSSCs are studied and compared...
September 21, 2016: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/27536525/anthropogenic-platinum-group-element-pt-pd-rh-concentrations-in-pm10-and-pm2-5-from-kolkata-india
#6
Huey Ting Diong, Reshmi Das, Bahareh Khezri, Bijayen Srivastava, Xianfeng Wang, Pradip K Sikdar, Richard D Webster
This study investigates platinum group elements (PGEs) in the breathable (PM10) and respirable (PM2.5) fractions of air particulates from a heavily polluted Indian metro city. The samples were collected from traffic junctions at the heart of the city and industrial sites in the suburbs during winter and monsoon seasons of 2013-2014. PGE concentrations were determined by inductively coupled plasma-mass spectrometry (ICP-MS). The PGE concentrations in the samples from traffic junctions are within the range of 2...
2016: SpringerPlus
https://www.readbyqxmd.com/read/27494359/local-brain-activity-persists-during-apparently-generalized-postictal-eeg-suppression
#7
Dirk-Matthias Altenmüller, Andreas Schulze-Bonhage, Christian E Elger, Rainer Surges
OBJECTIVES: Postictal generalized EEG suppression (PGES) frequently occurs after generalized convulsive seizures (GCS) and may be involved in the pathophysiology of sudden unexpected death in epilepsy (SUDEP). It is usually determined using conventional scalp EEG which is likely to miss cerebral activity in deeper brain structures. Here, we examined intracranial EEG activity after GCS to unravel the pattern and extent of local brain activity during apparent PGES on scalp EEG (s-PGES)...
September 2016: Epilepsy & Behavior: E&B
https://www.readbyqxmd.com/read/27488032/the-effect-of-coumestrol-on-progesterone-and-prostaglandin-production-in-the-mare-in-vitro-and-in-vivo-studies
#8
Anna Z Szóstek, Agnieszka Sadowska, Katarzyna K Piotrowska-Tomala, Marta Botelho, Maria João Fradinho, Maria Rosa Rebordão, Graça M Ferreira-Dias, Dariusz J Skarzynski
Coumestrol (Cou) is a plant-derived phytoestrogen that induces various pathologies in the female reproductive tract. Although effects of phytoestrogens on reproductive function in other species are well documented, their influence on progesterone (P4) and prostaglandin (PG) secretion in the mare is unknown. The aim of this study was to determine if Cou directly affects P4 and PG concentrations (in vivo) and endometrial PG secretion (in vitro) in the mare. In Experiment 1, the mares (n=4) were fed for 14 days with a diet containing increasing proportions of alfalfa pellets (250 g to 1 kg/day)...
August 3, 2016: Biology of Reproduction
https://www.readbyqxmd.com/read/27477116/ppar-ligand-association-with-prostaglandin-f2%C3%AE-and-e2-synthesis-in-the-pig-corpus-luteum-an-in-vitro-study
#9
A Kurzynska, K Chojnowska, M Bogacki, I Bogacka
The present study evaluated the involvement of PPARs in prostaglandin (PG) E2 and F2α production in the corpus luteum (CL) of pigs on days 10-12 and 14-16 of the estrous cycle or pregnancy. The tissue explants were incubated for 6h in the presence of PPARα, PPARβ, PPARγ ligands. The concentration of PGs in the incubation media was determined by radioimmunoassay, while mRNA abundance of PG synthetases (PGES and PGFS) was analyzed by quantitative real-time PCR. It was found that L-165,045 and rosiglitazone stimulated PGES synthesis on days 10-12 of the estrous cycle, whereas all factors that were assessed did not affect PGE2 release...
September 2016: Animal Reproduction Science
https://www.readbyqxmd.com/read/27431225/emergence-and-plasmid-analysis-of-klebsiella-pneumoniae-kp01-carrying-blages-5-from-guangzhou-china
#10
Ding-Qiang Chen, Ai-Wu Wu, Ling Yang, Dan-Hong Su, Yong-Ping Lin, Yan-Wei Hu, Lei Zheng, Qian Wang
Klebsiella pneumoniae strain KP01 carrying blaGES-5 was identified from a patient in Guangzhou, China. High-throughput sequencing assigned blaGES-5 to a 28.5-kb nonconjugative plasmid, pGES-GZ. A 13-kb plasmid backbone sequence on pGES-GZ was found to share high sequence identities with plasmids from Gram-negative nonfermenters. A novel class 1 integron carrying a gene cassette array of orf28-orf28-blaGES-5 was identified on pGES-GZ, within which orf28 encoded a hypothetical protein possibly correlated to fosfomycin resistance...
October 2016: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/27357326/automated-video-detection-of-epileptic-convulsion-slowing-as-a-precursor-for-post-seizure-neuronal-collapse
#11
Stiliyan N Kalitzin, Prisca R Bauer, Robert J Lamberts, Demetrios N Velis, Roland D Thijs, Fernando H Lopes Da Silva
Automated monitoring and alerting for adverse events in people with epilepsy can provide higher security and quality of life for those who suffer from this debilitating condition. Recently, we found a relation between clonic slowing at the end of a convulsive seizure (CS) and the occurrence and duration of a subsequent period of postictal generalized EEG suppression (PGES). Prolonged periods of PGES can be predicted by the amount of progressive increase of interclonic intervals (ICIs) during the seizure. The purpose of the present study is to develop an automated, remote video sensing-based algorithm for real-time detection of significant clonic slowing that can be used to alert for PGES...
April 4, 2016: International Journal of Neural Systems
https://www.readbyqxmd.com/read/27179861/novel-concepts-on-the-role-of-prostaglandins-on-luteal-maintenance-and-maternal-recognition-and-establishment-of-pregnancy-in-ruminants
#12
Joe A Arosh, Sakhila K Banu, John A McCracken
In ruminants, the corpus luteum (CL) of early pregnancy is resistant to luteolysis. Prostaglandin (PG)E2 is considered a luteoprotective mediator. Early studies indicate that during maternal recognition of pregnancy (MRP) in ruminants, a factor(s) from the conceptus or gravid uterus reaches the ovary locally through the utero-ovarian plexus (UOP) and protects the CL from luteolysis. The local nature of the embryonic antiluteolytic or luteoprotective effect precludes any direct effect of a protein transported or acting between the gravid uterus and CL in ruminants...
July 2016: Journal of Dairy Science
https://www.readbyqxmd.com/read/27043176/non-microbial-sources-of-microbial-volatile-organic-compounds
#13
Hyunok Choi, Norbert Schmidbauer, Carl-Gustaf Bornehag
BACKGROUND: The question regarding the true sources of the purported microbial volatile organic compounds (MVOCs) remains unanswered. OBJECTIVE: To identify microbial, as well as non-microbial sources of 28 compounds, which are commonly accepted as microbial VOCs (i.e. primary outcome of interest is Σ 28 VOCs). METHODS: In a cross-sectional investigation of 390 homes, six building inspectors assessed water/mold damage, took air and dust samples, and measured environmental conditions (i...
July 2016: Environmental Research
https://www.readbyqxmd.com/read/27035829/effects-of-1-25-dihydroxy-vitamin-d3-on-endometriosis
#14
Mariko Miyashita, Kaori Koga, Gentaro Izumi, Fusako Sue, Tomoko Makabe, Ayumi Taguchi, Miwako Nagai, Yoko Urata, Masashi Takamura, Miyuki Harada, Tetsuya Hirata, Yasushi Hirota, Osamu Wada-Hiraike, Tomoyuki Fujii, Yutaka Osuga
CONTEXT: Endometriosis is an estrogen-dependent, chronic inflammatory disease. Recent studies have shown that vitamin D (VD) is an effective modulator of the immune system and plays an important role in controlling many inflammatory diseases. OBJECTIVE: The objective of the study was to clarify the in vitro effects of 1,25-dihydroxy vitamin D3 (1,25[OH]2D3) on human endometriotic stromal cells (ESCs) and to determine the serum levels of VD in endometriosis patients...
June 2016: Journal of Clinical Endocrinology and Metabolism
https://www.readbyqxmd.com/read/27033056/misoprostol-reverse-hippocampal-neuron-cyclooxygenase-2-downstream-signaling-imbalance-in-aluminum-overload-rats
#15
Yuanxin Guo, Wenjuan Lei, Jianfeng Wang, Xinyue Hu, Yuling Wei, Chaonan Ji, Junqing Yang
Although COX-2 inhibition in animal models of neurodegenerative diseases has shown neuroprotection, recent studies have revealed some serious side effects (ulcers, bleeding, fatal cerebrovascular diseases etc.) and the limited benefits of COX-2 inhibitors. A more focused approach is necessary to explore the therapeutic effect of the COX downstream signaling pathway in neurological research. The aim of this study was to explore the alterations of the PGES-PGE2-EP signal pathway and the effect of misoprostol on neurodegeneration by chronic aluminum-overload in rats...
2016: Current Alzheimer Research
https://www.readbyqxmd.com/read/27013739/chondroitin-sulfate-immobilized-on-a-biomimetic-scaffold-modulates-inflammation-while-driving-chondrogenesis
#16
Bruna Corradetti, Francesca Taraballi, Silvia Minardi, Jeffrey Van Eps, Fernando Cabrera, Lewis W Francis, Salvatore A Gazze, Mauro Ferrari, Bradley K Weiner, Ennio Tasciotti
UNLABELLED: Costs associated with degenerative inflammatory conditions of articular cartilage are exponentially increasing in the aging population, and evidence shows a strong clinical need for innovative therapies. Stem cell-based therapies represent a promising strategy for the treatment of innumerable diseases. Their regenerative potential is undeniable, and it has been widely exploited in many tissue-engineering approaches, especially for bone and cartilage repair. Their immune-modulatory capacities in particular make stem cell-based therapeutics an attractive option for treating inflammatory diseases...
May 2016: Stem Cells Translational Medicine
https://www.readbyqxmd.com/read/26994879/impact-of-periictal-nurse-interventions-on-postictal-generalized-eeg-suppression-in-generalized-convulsive-seizures
#17
Shasha Wu, Naoum P Issa, Sandra L Rose, Ahmer Ali, James X Tao
OBJECTIVES: The objective of this study was to determine the impact of periictal nurse interventions on postictal generalized EEG suppression (PGES) in generalized convulsive seizures (GCS). METHODS: We retrospectively reviewed the video-EEG recordings of patients during long-term video-EEG monitoring. We compared the duration of seizures, seizure phases (tonic, clonic, and tonic-clonic phases), and the occurrence and duration of postictal generalized EEG suppression (PGES) in patients with and without periictal interventions (e...
May 2016: Epilepsy & Behavior: E&B
https://www.readbyqxmd.com/read/26992524/high-specific-surface-gold-electrode-on-polystyrene-substrate-characterization-and-application-as-dna-biosensor
#18
Zhiliu Yang, Yichen Liu, Wei Lu, Qingpan Yuan, Wei Wang, Qiaosheng Pu, Bo Yao
In the past decades, many efforts have been made to improve the sensitivity and specificity of electrochemical DNA biosensors. However, it is still strongly required to develop disposable and reliable DNA biosensors for wide and practical application. In this article, we reported superior electrochemical properties of an integrated plastic-gold electrode (PGE) fabricated in-house by chemical plating on polystyrene substrate. PGEs were found having extremely high capacity of DNA immobilization compared with gold electrodes fabricated by standard sputtering based photolithography...
May 15, 2016: Talanta
https://www.readbyqxmd.com/read/26923046/intramammary-lipopolysaccharide-infusion-alters-gene-expression-but-does-not-induce-lysis-of-the-bovine-corpus-luteum
#19
J Lüttgenau, O Wellnitz, D Kradolfer, E Kalaitzakis, S E Ulbrich, R M Bruckmaier, H Bollwein
Data from various studies indicate that the ovarian function in dairy cows can be compromised during intramammary infections. Therefore, in this study, we investigated if an experimentally induced mastitis has an effect on corpus luteum (CL) function in 14 lactating cows. On d 9 of the estrous cycle (d 1=ovulation), cows received a single dose of 200 μg of Escherichia coli lipopolysaccharide (LPS; dissolved in 10 mL of NaCL; n=8) or 10 mL of saline (control; n=6) into one quarter of the mammary gland. Measurements included plasma cortisol, haptoglobin, and progesterone (P4) concentrations, as well as luteal size (LTA) and relative luteal blood flow (rLBF)...
May 2016: Journal of Dairy Science
https://www.readbyqxmd.com/read/26874614/the-ph-dependent-release-of-platinum-group-elements-pges-from-gasoline-and-diesel-fuel-catalysts-implication-for-weathering-in-soils
#20
Veronika Suchá, Martin Mihaljevič, Vojtěch Ettler, Ladislav Strnad
Powdered samples of new and old gasoline catalysts (Pt, Pd, Rh) and new and old diesel (Pt) catalysts were subjected to a pH-static leaching procedure (pH 2-9) coupled with thermodynamic modeling using PHREEQC-3 to verify the release and mobility of PGEs (platinum group elements). PGEs were released under acidic conditions, mostly exhibiting L-shaped leaching patterns: diesel old: 5.47, 0.005, 0.02; diesel new: 68.5, 0.23, 0.11; gasoline old: 0.1, 11.8, 4.79; gasoline new 2.6, 25.2, 35.9 in mg kg(-1) for Pt, Pd and Rh, respectively...
April 15, 2016: Journal of Environmental Management
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