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Mycoplasma genitalium

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https://www.readbyqxmd.com/read/28418319/increasing-macrolide-and-fluoroquinolone-resistance-in-mycoplasma-genitalium
#1
Gerald L Murray, Catriona S Bradshaw, Melanie Bissessor, Jennifer Danielewski, Suzanne M Garland, Jørgen S Jensen, Christopher K Fairley, Sepehr N Tabrizi
Escalating resistance to azithromycin and moxifloxacin is being reported for Mycoplasma genitalium in the Asia-Pacific region. Analyzing 140 infections, we found pretreatment fluoroquinolone-resistance mutations in parC (13.6%) and gyrA (5%). ParC S83 changes were associated with moxifloxacin failure. Combined macrolide/fluoroquinolone-resistance mutations were in 8.6% of specimens, for which recommended therapies would be ineffective.
May 2017: Emerging Infectious Diseases
https://www.readbyqxmd.com/read/28407014/prevalence-of-macrolide-and-fluoroquinolone-resistance-mediating-mutations-in-mycoplasma-genitalium-in-five-cities-in-russia-and-estonia
#2
Elena Shipitsyna, Tatiana Rumyantseva, Daniel Golparian, Guzel Khayrullina, Amaya C Lagos, Inna Edelstein, Kai Joers, Jörgen S Jensen, Alevtina Savicheva, Natalia Rudneva, Larisa Sukhanova, Roman Kozlov, Alexander Guschin, Magnus Unemo
BACKGROUND AND OBJECTIVE: Resistance in the sexually transmitted bacterium Mycoplasma genitalium to all recommended therapeutic antimicrobials have rapidly emerged. However, to date, internationally reported resistance surveillance data for M. genitalium strains circulating in Eastern Europe are entirely lacking. The aim of this study was to estimate the prevalence of macrolide and fluoroquinolone resistance-associated mutations in M. genitalium in four cities in Russia and one in Estonia, 2013-2016...
2017: PloS One
https://www.readbyqxmd.com/read/28381611/prospective-evaluation-of-resistanceplus%C3%A2-mg-a-new-multiplex-qpcr-assay-for-the-detection-of-mycoplasma-genitalium-and-macrolide-resistance
#3
S N Tabrizi, J Su, C S Bradshaw, C K Fairley, S Walker, L Y Tan, E Mokany, S M Garland
Mycoplasma genitalium is a significant pathogen for which first-line treatment is becoming less effective due to increased resistance to macrolides. As conventional culture and antimicrobial susceptibility testing is not feasible for routine detection of this pathogen, molecular markers such as detection of mutations in the 23S rRNA gene have been described to predict resistance. Recently a novel multiplex qPCR assay, ResistancePlus™ MG, has been described for the simultaneous detection of Mycoplasma genitalium and macrolide resistance...
April 5, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28377334/mycoplasma-genitalium-a-mycoplasma-still-underestimated
#4
Julie Plantamura, Christine Bigaillon, Aurore Bousquet, Déborah Delaune, Sébastien Larréché, Sarah Bugier, Audrey Mérens, Cécile Ficko
Three men referred to the emergency department with suspected sexually transmitted infection like urethritis. After collection of several clinical specimens, they are sent home with a probabilistic treatment. Mycoplasma genitalium research is performed in first line, as Neisseria gonorrhoeae and Chlamydia trachomatis, and comes back positive. Patients are recalled in order to evaluate probabilistic treatment efficiency. M. genitalium, still underestimated because of its recent discovery, is responsible for 10 to 35% of non gonococcal acute and chronical urethritis...
April 1, 2017: Annales de Biologie Clinique
https://www.readbyqxmd.com/read/28361246/multidrug-resistant-mycoplasma-genitalium-infections-in-europe
#5
J F Braam, L van Dommelen, C J M Henquet, J H B van de Bovenkamp, J G Kusters
In Japan and Australia, multidrug-resistant Mycoplasma genitalium infections are reported with increasing frequency. Although macrolide-resistant M. genitalium strains are common in Europe and North America, fluoroquinolone-resistant strains are still exceptional. However, an increase of multidrug-resistant M. genitalium in Europe and America is to be expected. The aim of this paper is to increase awareness on the rising number of multidrug-resistant M. genitalium strains. Here, one of the first cases of infection with a genetically proven multidrug-resistant M...
March 30, 2017: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/28301989/routine-testing-of-mycoplasma-genitalium-and-trichomonas-vaginalis
#6
Marieke L M Jenniskens, Jan H W Veerbeek, Koen L Deurloo, Erik J van Hannen, Steven F T Thijsen
BACKGROUND: Mycoplasma genitalium (MG) and Trichomonas vaginalis (TV) are common sexual transmitted infections (STI). However, most STI screening programmes do not include routinely detection of these pathogens. Consequently, epidemiological data about MG and TV in the general population is lacking. The current study aims to give insight into the prevalence of both infections, thereby guiding decisions whether testing for these pathogens should be included routinely. METHODS: Between February 2013 and August 2015, all samples sent to the laboratory of Diakonessenhuis Utrecht for STI testing (i...
June 2017: Infectious Diseases
https://www.readbyqxmd.com/read/28291669/mycoplasma-genitalium-in-spain-prevalence-of-genital-infection-and-frequency-of-resistance-to-macrolides
#7
Alejandra Asenjo, Johannes G Kusters, Tim T Severs, Juan-Ignacio Alós
INTRODUCTION: The aim of this study was to determine the prevalence of Mycoplasma genitalium infection and the resistance to macrolides within a general population in Madrid in 2015. METHODS: We collected 359 urine samples from a general population with symptoms of sexually transmitted infections (STIs). All samples underwent a real-time PCR. For the detection of macrolide resistance, a 283bp fragment of region V of the 23S rRNA gene of M. genitalium was amplified and sequenced...
March 11, 2017: Enfermedades Infecciosas y Microbiología Clínica
https://www.readbyqxmd.com/read/28268197/microbial-invasion-of-the-amniotic-cavity-in-midtrimester-pregnancies-using-molecular-microbiology
#8
Shelley Rowlands, Jennifer A Danielewski, Sepehr N Tabrizi, Susan P Walker, Suzanne M Garland
OBJECTIVE: The objective of the study was to determine the frequency of microbial invasion of the amniotic cavity in the midtrimester of pregnancy in patients undergoing amniocentesis for clinical indications. STUDY DESIGN: This was a prospective investigation of the amniotic fluid of 344 asymptomatic women recruited in midpregnancy for the presence of microbial DNA. Amniotic samples obtained at the time of amniocentesis for genetic testing on women between 15 and 22 weeks of gestation were tested specifically for the presence of Ureaplasma urealyticum, Ureaplasma parvum, Mycoplasma hominis, and Mycoplasma genitalium as well as for other bacteria and fungi using broad-range polymerase chain reaction only...
March 6, 2017: American Journal of Obstetrics and Gynecology
https://www.readbyqxmd.com/read/28263902/mollicutes-in-vaginal-microbiology-mycoplasma-hominis-ureaplasma-urealyticum-ureaplasma-parvum-and-mycoplasma-genitalium
#9
David Taylor-Robinson
Mycoplasma hominis was isolated in 1937 from the human genital tract, followed 17 years later by Ureaplasma urealyticum and 27 years after that by Mycoplasma genitalium. The first two proved relatively easy to culture but the latter required a polymerase chain reaction assay for further studies. In sexually mature women, M. hominis may be found in the vagina/cervix of about 20-50%, ureaplasmas in 40-80% and M. genitalium in 0-5%. Some heterogeneity has been found among strains of all these species, sufficient to divide ureaplasmas into two species, namely U...
March 2, 2017: Research in Microbiology
https://www.readbyqxmd.com/read/28260146/simultaneous-detection-and-identification-of-sti-pathogens-by-multiplex-real-time-pcr-in-genital-tract-specimens-in-a-selected-area-of-apulia-a-region-of-southern-italy
#10
Raffaele Del Prete, Luigi Ronga, Mirella Lestingi, Grazia Addati, Umberto Filippo Angelotti, Domenico Di Carlo, Giuseppe Miragliotta
PURPOSE: Genital tract infections are globally a major cause of morbidity in sexually active individuals. The aim of this study was to investigate the prevalence and associations of co-infections of Chlamydia trachomatis, Neisseria gonorrhoeae, Trichomonas vaginalis, Mycoplasma hominis (MH), Mycoplasma genitalium, Ureaplasma urealyticum (UU) and Ureaplasma parvum (UP) in specimens collected from female (SF) and male (SM) patients. METHODS: 1575 samples from 1575 individuals from the geographical area around Bari, Apulia region in Southern Italy, were collected and analyzed by a multiplex Real-Time PCR (mRT-PCR) (Anyplex(TM) II STI-7, Seegene, Inc...
March 4, 2017: Infection
https://www.readbyqxmd.com/read/28178118/microscopy-of-stained-urethral-smear-in-male-urethritis-which-cutoff-should-be-used
#11
Harald Moi, Usha Hartgill, Kristin Helene Skullerud, Elina J Reponen, Line Syvertsen, Amir Moghaddam
BACKGROUND: The microscopical diagnosis of male urethritis was recently questioned by Rietmeijer and Mettenbrink, lowering the diagnostic criteria of the diagnosis to ≥2 polymorphonuclear leucocytes (PMNL) per high power field (HPF), and adopted by Centers for Disease Control and Prevention in their 2015 STD Treatment Guidelines. The European Non-Gonococcal Urethritis Guideline advocates a limit of ≥5 PMNL/HPF. OBJECTIVE: To determine if syndromic treatment of urethritis should be considered with a cutoff value of ≥2 PMNL/HPF in urethral smear...
March 2017: Sexually Transmitted Diseases
https://www.readbyqxmd.com/read/28159916/impact-of-deploying-multiple-point-of-care-tests-with-a-sample-first-approach-on-a-sexual-health-clinical-care-pathway-a-service-evaluation
#12
Emma M Harding-Esch, Achyuta V Nori, Aseel Hegazi, Marcus J Pond, Olanike Okolo, Anthony Nardone, Catherine M Lowndes, Phillip Hay, S Tariq Sadiq
OBJECTIVES: To assess clinical service value of STI point-of-care test (POCT) use in a 'sample first' clinical pathway (patients providing samples on arrival at clinic, before clinician consultation). Specific outcomes were: patient acceptability; whether a rapid nucleic acid amplification test (NAAT) for Chlamydia trachomatis/Neisseria gonorrhoeae (CT/NG) could be used as a POCT in practice; feasibility of non-NAAT POCT implementation for Trichomonas vaginalis (TV) and bacterial vaginosis (BV); impact on patient diagnosis and treatment...
February 3, 2017: Sexually Transmitted Infections
https://www.readbyqxmd.com/read/28158595/high-prevalence-of-the-a2058t-macrolide-resistance-associated-mutation-in-mycoplasma-genitalium-strains-from-the-netherlands
#13
Joyce F Braam, Brenda Slotboom, Sebastian Van Marm, Tim T Severs, Noortje M Van Maarseveen, Ton Van Zwet, Edwin C H Boel, Hanneke Berkhout, Ferry Hagen, Jeroen H B Van De Bovenkamp, Laura Van Dommelen, Johannes G Kusters
No abstract text is available yet for this article.
February 3, 2017: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/28118803/meta-analysis-of-the-efficacy-of-moxifloxacin-in-treating-mycoplasma-genitalium-infection
#14
Yang Li, Wen-Jing Le, Sai Li, Yu-Ping Cao, Xiao-Hong Su
Mycoplasma genitalium is an important pathogen that is transmitted through sexual contact. For patients diagnosed with M. genitalium infection, the current guidelines recommend 1 g of azithromycin as the first-line treatment. Moxifloxacin is used as a second-line drug due to its remarkable efficacy; however, increased use of moxifloxacin to treat M. genitalium infections has caused the emergence of cases of moxifloxacin treatment failure. This meta-analysis aims to estimate the treatment efficacy of moxifloxacin for M...
January 1, 2017: International Journal of STD & AIDS
https://www.readbyqxmd.com/read/28114309/superior-multiplexing-capacity-of-plexprimers-enables-sensitive-and-specific-detection-of-snps-and-clustered-mutations-in-qpcr
#15
Lit Yeen Tan, Samantha Michelle Walker, Tina Lonergan, Nicole Elizabeth Lima, Alison Velyian Todd, Elisa Mokany
BACKGROUND: Whilst qPCR provides an extremely powerful tool for genetic analysis, some applications such as multiplexing variant alleles (eg SNPs, point mutations or deletions), remain challenging using current primer/probe systems. The novel design features of PlexPrimers allow sensitive, multiplexed analysis of variant alleles even when these are tightly clustered. METHOD: PlexPrimers were combined with PlexZymes in qPCR assays for the detection of SNPs in human absorption, distribution, metabolism, and excretion (ADME) genes; clustered mutations in the 23S rRNA gene which confer antibiotic resistance to Mycoplasma genitalium; and deletions within the human epidermal growth factor receptor (EGFR) gene...
2017: PloS One
https://www.readbyqxmd.com/read/28112332/-molecular-detection-of-sexually-transmitted-agents-in-a-symptomatic-group-of-men-and-its-relationship-with-sexual-behavior
#16
Daniela León, Javier Retamal, Ramón Silva, Carmen Ili, Stephanie Mieville, Pablo Guzmán, Gastón Briceño, Priscilla Brebi
BACKGROUND: Sexually transmitted infections (STIs) affect sexual and reproductive health of millions of men. Pathogens such as human papillomavirus (HPV), herpes simplex virus type 1 and 2 (HSV-1 y HSV-2), Chlamydia trachomatis,Mycoplasmagenitalium,Mycoplasma hominis and Ureaplasma urealyticum are associated with STIs. AIM: To detect pathogens associated with STIs in symptomatic men and its relationship with sexual behavior. METHODOLOGY: DNA was obtained from exfoliated cells of penis from 20 symptomatic men...
October 2016: Revista Chilena de Infectología: órgano Oficial de la Sociedad Chilena de Infectología
https://www.readbyqxmd.com/read/28099135/mycoplasma-ureaplasma-infection-in-pregnancy-to-screen-or-not-to-screen
#17
Gilbert G G Donders, Kateryna Ruban, Gert Bellen, Ljubomir Petricevic
Mycoplasmata have been linked to pregnancy complications and neonatal risk. While formerly a limited number of species could be discovered by cultures, molecular biology nowadays discovers both lower quantities and more diverse species, making us realize that mycoplasmata are ubiquitous in the vaginal milieu and do not always pose a danger for pregnant women. As the meaning of mycoplasmata in pregnancy is not clear to many clinicians, we summarized the current knowledge about the meaning of different kinds of mycoplasmata in pregnancy and discuss the potential benefits and disadvantages of treatment...
January 18, 2017: Journal of Perinatal Medicine
https://www.readbyqxmd.com/read/28079749/should-female-partners-of-men-with-non-gonococcal-urethritis-negative-for-chlamydia-trachomatis-and-mycoplasma-genitalium-be-informed-and-treated-clinical-outcomes-from-a-partner-study-of-heterosexual-men-with-ngu
#18
Jason J Ong, Angela Sarumpaet, Eric P F Chow, Catriona Bradshaw, Marcus Chen, Tim Read, Christopher K Fairley
BACKGROUND: To determine if female partners of men with pathogen-negative non-gonococcal urethritis (NGU) are at risk of genital infection. METHODS: Secondary data analysis using health records from a large sexually transmitted disease clinic in Melbourne of 1710 men and their female partners attending on the same day from January 2006 to April 2015. Proportions of female partners with symptoms suggesting genital infection or pelvic inflammatory disease (PID) were determined for: (1) men with NGU and no Chlamydia trachomatis or Mycoplasma genitalium (referred to as pathogen-negative NGU) (n = 91); 2) men with urethral C...
February 2017: Sexually Transmitted Diseases
https://www.readbyqxmd.com/read/28072403/antimicrobial-resistant-sexually-transmitted-infections-gonorrhoea-and-mycoplasma-genitalium
#19
REVIEW
Magnus Unemo, Jorgen S Jensen
The emergence of antimicrobial resistance (AMR) is a major concern worldwide and already compromises treatment effectiveness and control of several bacterial sexually transmitted infections (STIs). Neisseria gonorrhoeae and Mycoplasma genitalium are evolving into so-called superbugs that can become resistant, both in vitro and clinically, to essentially all antimicrobials available for treatment, causing exceedingly difficult-to-treat or untreatable STIs and threatening global public health. Widespread AMR in these bacteria is likely to persist and even worsen in the future, owing to the high number of infections, widespread and uncontrolled use of antimicrobials, limited surveillance of AMR and clinical failures, as well as the extraordinary capacity of these bacteria to develop AMR...
January 10, 2017: Nature Reviews. Urology
https://www.readbyqxmd.com/read/28032361/relationship-between-chlamydia-trachomatis-and-mycoplasma-genitalium-infection-and-pregnancy-rate-and-outcome-in-iranian-infertile-couples
#20
L Dehghan Marvast, A Aflatoonian, A R Talebi, A Eley, A A Pacey
The study was performed to investigate the prevalence of Chlamydia trachomatis and Mycoplasma genitalium in a population of infertile couples from Iran and how this relates to tubal factor infertility, pregnancy rate and outcome of pregnancy. Blood, semen and first-void urine samples were obtained from 250 infertile couples and 250 fertile women as a control. Infertile couples were followed up after 24 months to determine diagnosis, referral for assisted conception, any pregnancy and pregnancy outcome. Data were analysed with regard to the results of (i) serological analysis for specific antibodies to C...
December 29, 2016: Andrologia
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