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Mycoplasma genitalium

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https://www.readbyqxmd.com/read/27898565/quantitative-real-time-polymerase-chain-reaction-for-the-diagnosis-of-mycoplasma-genitalium-infection-in-south-african-men-with-and-without-symptoms-of-urethritis
#1
Marie Cecilia le Roux, Anwar Ahmed Hoosen
BACKGROUND: This study was done to diagnose Mycoplasma genitalium infection based on bacterial load in urine specimens from symptomatic and asymptomatic men. METHODS: Urine specimens from 94 men with visible urethral discharge, 206 with burning on micturition and 75 without symptoms presenting to a family practitioner were tested for M. genitalium as well as Neisseria gonorrhoeae, Chlamydia trachomatis, Trichomonas vaginalis by transcription-mediated amplification assays...
November 28, 2016: Sexually Transmitted Diseases
https://www.readbyqxmd.com/read/27894204/clinical-laboratory-assessments-for-mycoplasma-genitalium-in-a-high-prevalence-sexually-transmitted-infection-community-reveal-epidemiologic-dichotomies-with-trichomonas-vaginalis
#2
Erik Munson, Kimber L Munson, Ronald F Schell
Mycoplasma genitalium is an emerging agent of sexually-transmitted infection and is responsible for clinically-significant genital tract disease in both females and males. Similar to scenarios recently experienced with the urogenital flagellate Trichomonas vaginalis, an evolving molecular diagnostic reference standard based on transcription-mediated amplification allows for accurate detection of the organism, plus additional insight into disease epidemiology. Areas covered. The basis for this article includes primary peer-reviewed literature plus compilations of data derived from routine clinical laboratory screening of females and males for agents of sexually-transmitted infection...
November 29, 2016: Expert Review of Anti-infective Therapy
https://www.readbyqxmd.com/read/27893600/prevalence-and-correlates-of-genital-infections-among-newly-diagnosed-human-immunodeficiency-virus-infected-adults-entering-human-immunodeficiency-virus-care-in-windhoek-namibia
#3
Gaston Djomand, Madeleine Schlefer, Steve Gutreuter, Sarah Tobias, Roopal Patel, Nickolas DeLuca, Julia Hood, Souleymane Sawadogo, Cheng Chen, Alexinah Muadinohamba, David W Lowrance, Naomi Bock
BACKGROUND: Identifying and treating genital infections, including sexually transmitted infections (STI), among newly diagnosed human immunodeficiency virus (HIV)-infected individuals may benefit both public and individual health. We assessed prevalence of genital infections and their correlates among newly diagnosed HIV-infected individuals enrolling in HIV care services in Namibia. METHODS: Newly diagnosed HIV-infected adults entering HIV care at 2 health facilities in Windhoek, Namibia, were recruited from December 2012 to March 2014...
November 2016: Sexually Transmitted Diseases
https://www.readbyqxmd.com/read/27882384/prevalence-of-mycoplasma-genitalium-infection-and-relationship-with-symptoms-among-adults-attending-a-sexual-health-centre
#4
Maeva Lefebvre, Julie Coutherut, Sophie Gibaud, Charlotte Biron, Marine Chalopin, Claire Bernier, François Raffi
is missing (Short communication).
November 24, 2016: Acta Dermato-venereologica
https://www.readbyqxmd.com/read/27872239/experimental-infection-of-pig-tailed-macaques-macaca-nemestrina-with-mycoplasma-genitalium
#5
Gwendolyn E Wood, Dorothy L Patton, Peter K Cummings, Stefanie L Iverson-Cabral, Patricia A Totten
Mycoplasma genitalium is an underappreciated cause of human reproductive tract disease, characterized by persistent, often asymptomatic, infection. Building on our previous experiments using a single female pig-tailed macaque as a model for M. genitalium infection (G. E. Wood, S. L. Iverson-Cabral, D. L. Patton, P. K. Cummings, Y. T. Cosgrove Sweeney, and P. A. Totten, Infect Immun 81:2938-2951, 2013, doi: 10.1128/IAI.01322-12), we cervically inoculated eight additional animals, two of which were simultaneously inoculated in salpingeal tissue autotransplanted into abdominal pockets...
November 21, 2016: Infection and Immunity
https://www.readbyqxmd.com/read/27836808/mycoplasma-genitalium-and-trichomonas-vaginalis-in-france-a-point-prevalence-study-in-people-screened-for-sexually-transmitted-diseases
#6
Sabine Pereyre, Cécile Laurier Nadalié, Cécile Bébéar
OBJECTIVES: Mycoplasma genitalium and Trichomonas vaginalis are common causes of sexually transmitted infections but limited prevalence data are available in France. We aimed to evaluate the prevalence of M. genitalium and T. vaginalis infections and to assess prevalence by gender, age, sample collection sites and clinical symptoms. A multicentre collection of specimens was intended to obtain a nationwide overview of the epidemiology. METHODS: Between September 2014 and January 2015, 2652 consecutive urogenital specimens submitted to the microbiology diagnostic departments of 16 French university hospitals for Chlamydia trachomatis and Neisseria gonorrhoeae detection were collected...
November 8, 2016: Clinical Microbiology and Infection
https://www.readbyqxmd.com/read/27829780/bacterial-load-in-daily-urine-samples-of-patients-infected-with-mycoplasma-genitalium-mutation-analysis-and-response-to-treatment
#7
M Gossé, S A Nordbø, B Pukstad
Objective. Increasing macrolide resistant strains of Mycoplasma genitalium is a challenge, and to differentiate between treatment failure and reinfection a timely test of cure (TOC) is warranted. The aim of this study was to evaluate the best time for TOC after five days' treatment of Mycoplasma genitalium infection with azithromycin. Methods. Nineteen patients with positive PCR for Mycoplasma genitalium in urine provided urine samples daily for 2 weeks and on days 21, 28, and 35. Samples were tested by a commercial qPCR and by sequencing of the 23S rRNA gene...
2016: Infectious Diseases in Obstetrics and Gynecology
https://www.readbyqxmd.com/read/27812493/a-case-control-study-on-the-relationship-between-mycoplasma-genitalium-infection-in-women-with-normal-pregnancy-and-spontaneous-abortion-using-polymerase-chain-reaction
#8
Rashid Ramazanzadeh, Mazaher Khodabandehloo, Fariba Farhadifar, Samaneh Rouhi, Amjad Ahmadi, Shaho Menbari, Fariba Fallahi, Reza Mirnejad
OBJECTIVES: Mycoplasma genitalium infections are suggested as causes of a number of pathological outcomes in pregnant women. The aim of this study was to evaluate the frequency of M. genitalium infections among pregnant women and its association with spontaneous abortion. METHODS: In this case-control study we included 109 women with spontaneous abortion with a gestational age of 10-20 weeks (patients), and 109 women with normal pregnancy with a gestational age of 20-37 weeks (controls) in Sanandaj, Iran...
October 2016: Osong Public Health and Research Perspectives
https://www.readbyqxmd.com/read/27797534/thermodynamics-of-macromolecular-association-in-heterogeneous-crowding-environments-theoretical-and-simulation-studies-with-a-simplified-model
#9
Tadashi Ando, Isseki Yu, Michael Feig, Yuji Sugita
The cytoplasm of a cell is crowded with many different kinds of macromolecules. The macromolecular crowding affects the thermodynamics and kinetics of biological reactions in a living cell, such as protein folding, association, and diffusion. Theoretical and simulation studies using simplified models focus on the essential features of the crowding effects and provide a basis for analyzing experimental data. In most of the previous studies on the crowding effects, a uniform crowder size is assumed, which is in contrast to the inhomogeneous size distribution of macromolecules in a living cell...
October 31, 2016: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/27773499/-mycoplasma-genitalium
#10
I Alcaraz, N Dupin, M Janier, C Derancourt, B Milpied, A Bertolotti
No abstract text is available yet for this article.
October 20, 2016: Annales de Dermatologie et de Vénéréologie
https://www.readbyqxmd.com/read/27756249/ureaplasma-parvum-genotype-combined-vaginal-colonisation-with-candida-albicans-and-spontaneous-preterm-birth-in-an-australian-cohort-of-pregnant-women
#11
Matthew S Payne, Demelza J Ireland, Rory Watts, Elizabeth A Nathan, Lucy L Furfaro, Matthew W Kemp, Jeffrey A Keelan, John P Newnham
BACKGROUND: Detection of Ureaplasma, Mycoplasma and Candida spp. in the vagina during pregnancy has previously been associated with preterm birth (PTB). However, the prevalence of these microorganisms and the associated obstetric risks (likely to be population-specific) have not been determined in Australian women; furthermore, in the case of Ureaplasma spp., very few studies have attempted characterisation at the species level and none have examined genotype/serovar status to further refine risk assessment...
October 18, 2016: BMC Pregnancy and Childbirth
https://www.readbyqxmd.com/read/27752188/bacterial-etiology-of-sexually-transmitted-infections-at-a-sti-clinic-in-ghana-use-of-multiplex-real-time-pcr
#12
Augustina A Sylverken, Ellis Owusu-Dabo, Denis D Yar, Samson P Salifu, Nana Yaa Awua-Boateng, John H Amuasi, Portia B Okyere, Thomas Agyarko-Poku
BACKGROUND: Most sexually transmitted infection (STI) management efforts focus on the syndromic approach to diagnose and treat patients. However, most women with STIs have been shown to be entirely asymptomatic, or if symptoms exist, are often missed when either clinical or conventional bacteriologic diagnostic tools are employed. METHODS: We assessed the performance of a multiplex real time PCR assay to describe other potential pathogens that could be missed by conventional bacteriological techniques in 200 women attending a routine STI clinic in Kumasi, Ghana...
September 2016: Ghana Medical Journal
https://www.readbyqxmd.com/read/27707988/successful-outcome-of-macrolide-resistant-mycoplasma-genitalium-urethritis-after-spectinomycin-treatment-a-case-report
#13
Lars Falk, Jørgen Skov Jensen
No abstract text is available yet for this article.
September 28, 2016: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/27679992/antimicrobial-susceptibility-patterns-of-recent-cuban-mycoplasma-genitalium-isolates-determined-by-a-modified-cell-culture-based-method
#14
Brian A Mondeja, Nadia M Rodríguez, Brenda Barroto, Orestes Blanco, Jørgen S Jensen
Isolation of Mycoplasma genitalium from clinical specimens remains difficult and few strains are available for antimicrobial susceptibility testing. We describe the antimicrobial susceptibility of M. genitalium strains grown in Vero cell culture with first- and second- line antibiotics, using a modified cell-culture-based method. Macrolide- and -fluoroquinolone resistance determinants were detected by sequencing of the 23S and parC genes, respectively. Seven strains were examined, including three new, genetically distinct M...
2016: PloS One
https://www.readbyqxmd.com/read/27629896/expansion-of-comprehensive-screening-of-male-sexually-transmitted-infection-clinic-attendees-with-mycoplasma-genitalium-and-trichomonas-vaginalis-molecular-assessment-a-retrospective-analysis
#15
Erik Munson, David Wenten, Sheila Jhansale, Mary Kay Schuknecht, Nicki Pantuso, Joshua Gerritts, Aaron Steward, Kimber L Munson, Maureen Napierala, Deb Hamer
Of 1493 male sexually-transmitted infection (STI) clinic encounters in a high-prevalence STI community, 8.7% revealed detection of Chlamydia trachomatis and 6.6% revealed detection of Neisseria gonorrhoeae Additional Trichomonas vaginalis and Mycoplasma genitalium screening resulted in 17.4% and 23.9% of encounters positive for STI, respectively. STI agents were detected in 13.7% of urine specimens; addition of pharyngeal and rectal collections resulted in detection of STI agents in 19.0% and 23.9% of encounters, respectively...
September 14, 2016: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/27617108/recruitment-of-minority-adolescents-and-young-adults-into-randomised-clinical-trials-testing-the-design-of-the-technology-enhanced-community-health-nursing-tech-n-pelvic-inflammatory-disease-trial
#16
Maria Trent, Shang-En Chung, Charlotte Gaydos, Kevin D Frick, Jennifer Anders, Steven Huettner, Richard Rothman, Arlene Butz
PURPOSE: Pelvic inflammatory disease (PID) disproportionately affects adolescent and young adult (AYA) women and can negatively influence reproductive health trajectories. Few randomized controlled trials (RCTs) have focused on strategies to improve outpatient adherence or to reduce reproductive morbidity in this population. This paper describes the research methods and preliminary effectiveness of recruitment, retention, and intervention strategies employed in a novel RCT designed to test a technology-enhanced community-health nursing (TECH-N) intervention among urban AYA with PID...
August 2016: European Medical Journal. Reproductive Health
https://www.readbyqxmd.com/read/27605499/background-review-for-the-2016-european-guideline-on-mycoplasma-genitalium-infections
#17
J S Jensen, M Cusini, M Gomberg, H Moi
Mycoplasma genitalium is a cause of 10-35% of non-chlamydial non-gonococcal urethritis in men and in women, and is associated with cervicitis and pelvic inflammatory disease (PID). Transmission of M. genitalium occurs through direct mucosal contact. In women, symptoms include vaginal discharge, dysuria or symptoms of PID - abdominal pain and dyspareunia. In men, urethritis, dysuria and discharge predominates. Asymptomatic infections are frequent. In this review, we present the evidence base for the recommendations in the 2016 European guideline on M...
September 7, 2016: Journal of the European Academy of Dermatology and Venereology: JEADV
https://www.readbyqxmd.com/read/27599076/sexually-transmitted-pathogens-coinfections-and-risk-factors-in-patients-attending-obstetrics-and-gynecology-clinics-in-jalisco-mexico
#18
Néstor Casillas-Vega, Rayo Morfín-Otero, Santos García, Jorge Llaca-Díaz, Eduardo Rodríguez-Noriega, Adrián Camacho-Ortiz, Ma de la Merced Ayala-Castellanos, Soraya Mendoza-Olazarán, Samantha Flores-Treviño, Santiago Petersen-Morfín, Héctor J Maldonado-Garza, Francisco J Bosques-Padilla, Elvira Garza-González
OBJECTIVE: To determine the frequency of nine sexually transmitted pathogens, coinfections and risk factors in patients attending obstetrics and gynecology clinics in Jalisco, Mexico. MATERIALS AND METHODS: Samples from 662 patients attending obstetrics and gynecology clinics were analyzed. Treponema pallidum, HIV, and HCV were detected by serology. HPV was detected by Polimerase Chain Reaction (PCR), and its genotype was determined by Restriction Fragment Length Polymorphism (RFLP)...
August 2016: Salud Pública de México
https://www.readbyqxmd.com/read/27588273/study-of-the-prevalence-and-association-of-ocular-chlamydial-conjunctivitis-in-women-with-genital-infection-by-chlamydia-trachomatis-mycoplasma-genitalium-and-candida-albicans-attending-outpatient-clinic
#19
Rania Abdelmonem Khattab, Maha Mohssen Abdelfattah
AIM: To determine the association between chlamydial conjunctivitis and genital infection by Chlamydia trachomatis, Mycoplasma genitalium and Candida albicans, in addition to the possible relationship between cultured bacterial pathogens and oculogenital chlamydial infection. METHODS: This study was performed on 100 (50 symptomatic and 50 asymptomatic) women attending the Gynecological and Obstetric outpatient clinic of Alzahra hospital, Alazhar University. Simultaneously a conjunctival swab was taken from these patients...
2016: International Journal of Ophthalmology
https://www.readbyqxmd.com/read/27558163/urethral-inflammatory-response-to-ureaplasma-is-significantly-lower-than-to-mycoplasma-genitalium-and-chlamydia-trachomatis
#20
Harald Moi, Nils Reinton, Ivana Randjelovic, Elina J Reponen, Line Syvertsen, Amir Moghaddam
A non-syndromic approach to treatment of people with non-gonococcal urethritis (NGU) requires identification of pathogens and understanding of the role of those pathogens in causing disease. The most commonly detected and isolated micro-organisms in the male urethral tract are bacteria belonging to the family of Mycoplasmataceae, in particular Ureaplasma urealyticum and Ureaplasma parvum To better understand the role of these Ureaplasma species in NGU, we have performed a prospective analysis of male patients voluntarily attending a drop in STI clinic in Oslo...
August 24, 2016: International Journal of STD & AIDS
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