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https://www.readbyqxmd.com/read/29679605/circulating-cell-free-dna-in-patients-with-alveolar-echinococcosis
#1
Alice Baraquin, Eric Hervouet, Carine Richou, Pierre Flori, Paul Peixoto, Amel Azizi, Eric Delabrousse, Oleg Blagosklonov, Gerald Umhang, Solange Bresson-Hadni, Benoit Valot, Frédéric Grenouillet
Alveolar echinococcosis (AE) is a parasitic disease, due to Echinococcus multilocularis. Often compared to liver cancer, it develops by infiltration from its primary site to the surrounding tissue, and can then metastasize to other organs. Detection of circulating cell-free DNA (ccfDNA) is a useful analytical tool in oncology, for diagnosis, prognosis, and therapy monitoring. This study sought to investigate the presence of ccfDNA in patients with AE, and its potential usefulness for the evaluation of treatment efficiency...
April 18, 2018: Molecular and Biochemical Parasitology
https://www.readbyqxmd.com/read/29679564/frequencies-and-expression-levels-of-programmed-death-ligand-1-pd-l1-in-circulating-tumor-rna-ctrna-in-various-cancer-types
#2
Toshiyuki Ishiba, Andreas-Claudius Hoffmann, Joshua Usher, Yahya Elshimali, Todd Sturdevant, Mai Dang, Yolanda Jaimes, Rama Tyagi, Ronald Gonzalez, Mary Grino, Jacek K Pinski, Afsaneh Barzi, Luis E Raez, Wilfried E Eberhardt, Dirk Theegarten, Heinz-Josef Lenz, Hiroyuki Uetake, Peter V Danenberg, Kathleen Danenberg
BACKGROUND: Precision medicine and prediction of therapeutic response requires monitoring potential biomarkers before and after treatment. Liquid biopsies provide noninvasive prognostic markers such as circulating tumor DNA and RNA. Circulating tumor RNA (ctRNA) in blood is also used to identify mutations in genes of interest, but additionally, provides information about relative expression levels of important genes. In this study, we analyzed PD-L1 expression in ctRNA isolated from various cancer types...
April 18, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29678513/review-of-blood-based-colorectal-cancer-screening-how-far-are-circulating-cell-free-dna-methylation-markers-from-clinical-implementation
#3
REVIEW
Mai-Britt Worm Ørntoft
Colorectal cancer (CRC) is a leading cause of cancer related deaths worldwide, and late stages (III-IV) in particular have low 5-year survival rates. Stage shifting by CRC screening programs has proven effective by decreasing morbidity and mortality and in many countries national CRC screening programs have been implemented. Currently, European, Asian, and American authorities recommend screening for CRC using fecal occult blood testing, sigmoidoscopy, or colonoscopy. Because these approaches all have weaknesses (eg, poor compliance, high costs, test invasiveness), much effort has been put into the development of alternative screening approaches, many of which are blood-based...
March 2, 2018: Clinical Colorectal Cancer
https://www.readbyqxmd.com/read/29668342/sequencing-the-next-generation-of-glioblastomas
#4
Ivana Jovčevska
The most aggressive brain malignancy, glioblastoma, accounts for 60-70% of all gliomas and is uniformly fatal. According to the molecular signature, glioblastoma is divided into four subtypes (proneural, neural, classical, and mesenchymal), each with its own genetic background. The Cancer Genome Atlas project provides information about the most common genetic changes in glioblastoma. They involve mutations in TP53, TERT, and PTEN, and amplifications in EFGR, PDGFRA, CDK4, CDK6, MDM2, and MDM4. Recently, epigenetics was used to demonstrate the oncogenic roles of miR-124, miR-137, and miR-128...
April 18, 2018: Critical Reviews in Clinical Laboratory Sciences
https://www.readbyqxmd.com/read/29666058/early-detection-sequencing-assays-prove-highly-specific
#5
(no author information available yet)
Ultrasensitive DNA-sequencing methods proved more than 99% specific, according to initial findings from GRAIL's Circulating Cell-Free Genome Atlas Study. The data, presented at the American Association for Cancer Research Annual Meeting 2018, demonstrate the feasibility of designing a blood test for early cancer detection.
April 17, 2018: Cancer Discovery
https://www.readbyqxmd.com/read/29664127/cell-free-nucleic-acids-are-present-in-blood-products-and-regulate-genes-of-innate-immune-response
#6
Sophie Waldvogel Abramowski, Diderik Tirefort, Pierre Lau, Arthur Guichebaron, Sofiane Taleb, Christine Modoux, Coralie Lemoine Chaduc, Pascale Bruyere Cerdan, Pascale Roux Lombard, Thomas Lecompte, Olivier Preynat-Seauve
BACKGROUND: Extracellular nucleic acids circulate in plasma. They are expected to be present in manufactured blood products eligible for transfusion, but little is known about their biological activity on human cells. The aim of this study is to investigate whether cell-free nucleic acids (CFNAs) are present and biologically active in red blood cell units (RBCUs), fresh frozen plasmas, and platelet concentrates. STUDY DESIGN AND METHODS: CFNAs were extracted from RBCUs, fresh frozen plasma, and platelet concentrates...
April 17, 2018: Transfusion
https://www.readbyqxmd.com/read/29658031/fully-automated-on-site-isolation-of-cfdna-from-whole-blood-for-cancer-therapy-monitoring
#7
Chi-Ju Kim, Juhee Park, Vijaya Sunkara, Tae-Hyeong Kim, Yongjin Lee, Kyusang Lee, Mi-Hyun Kim, Yoon-Kyoung Cho
The potential utility of circulating tumour DNA (ctDNA) in patient blood for cancer diagnostics and real-time monitoring of disease progression is highly recognized. However, the lack of automated and efficient methods for cell-free DNA (cfDNA) isolation from peripheral blood has remained a challenge for broader acceptance of liquid biopsy in general clinical settings. Here, we demonstrate a lab-on-a-disc system equipped with newly developed, electromagnetically actuated, and reversible diaphragm valves that allows fully automated and rapid (<30 min) isolation of cfDNA from whole blood (>3 ml) to achieve high detection sensitivity by minimizing the degradation of fragile ctDNA as well as contamination of wild-type DNA from abundant blood cells...
April 16, 2018: Lab on a Chip
https://www.readbyqxmd.com/read/29656868/dynamics-of-egfr-mutation-load-in-plasma-for-prediction-of-treatment-response-and-disease-progression-in-patients-with-egfr-mutant-lung-adenocarcinoma
#8
Álvaro Taus, Laura Camacho, Pedro Rocha, Max Hardy-Werbin, Lara Pijuan, Gabriel Piquer, Eva López, Alba Dalmases, Raquel Longarón, Sergi Clavé, Marta Salido, Joan Albanell, Beatriz Bellosillo, Edurne Arriola
BACKGROUND: The assessment of epidermal growth factor receptor (EGFR) mutations is crucial for the management of patients with lung adenocarcinoma. Circulating tumor DNA (ctDNA)-based assessment offers advantages over tumor as a minimally invasive method able to capture tumor heterogeneity. PATIENTS AND METHODS: Consecutive patients diagnosed with EGFR-mutant lung adenocarcinoma in tumor biopsy were included in this study. Plasma samples were obtained at different time points during the course of the disease...
March 23, 2018: Clinical Lung Cancer
https://www.readbyqxmd.com/read/29653118/acute-high-intensity-interval-exercise-induces-comparable-levels-of-circulating-cell-free-dna-and-interleukin-6-in-obese-and-normal-weight-individuals
#9
Peter J Ferrandi, Brandon G Fico, Michael Whitehurst, Michael C Zourdos, Fanchen Bao, Katelyn M Dodge, Alexandra A Rodriguez, Gabriel Pena, Chun-Jung Huang
AIMS: Obesity is associated with lipid aggregation in adipocytes and macrophage infiltration, leading to increased oxidative stress and inflammation. Increased cell-free DNA (cfDNA) concentrations have been observed in clinical conditions of systemic inflammation. While the beneficial effects of regular physical activity on the release of circulating cfDNA still remain unknown, acute intense exercise has been shown to increase inflammatory cytokines and cfDNA concentrations in normal-weight individuals...
April 10, 2018: Life Sciences
https://www.readbyqxmd.com/read/29644378/a-simple-and-ultrasensitive-fluorescence-assay-for-single-nucleotide-polymorphism
#10
Qian Ma, Zhiqiang Gao
In this report, a simple, label-free and highly efficient nucleic acid amplification technique is developed for ultrasensitive detection of single-nucleotide polymorphism (SNP). Briefly, a designed padlock probe is first circularized by a DNA ligase when it perfectly complements to a mutant gene. Then, the mutant gene functions as a primer to initiate branched rolling circle amplification reaction (BRCA), generating a large number of branched DNA strands and a lot of pyrophosphate molecules which is equivalent to the number of nucleotides consumed...
April 12, 2018: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/29630031/elevated-cell-free-mitochondrial-dna-in-filtered-plasma-is-associated-with-hiv-infection-and-inflammation
#11
Omair Arshad, Izabella Gadawska, Beheroze Sattha, Hélène C F Côté, Anthony Y Y Hsieh
BACKGROUND: Increased cell-free DNA levels are associated with poor health outcomes, and cell-free mitochondrial DNA (cf-mtDNA) has proinflammatory properties. Given that HIV infection is associated with chronic inflammation, we investigated the relationship between cf-mtDNA and proinflammatory cytokine interleukin-6 (IL-6) in the context of HIV infection. We also optimized separation of cell-free plasma from blood. SETTING: In this retrospective cross-sectional study, we collected blood, demographic information, and clinical data from 99 HIV-infected and 103 HIV-uninfected adults and children enrolled in the Children and Women: AntiRetrovirals and Markers of Aging pan-Canadian (CARMA) cohort...
May 1, 2018: Journal of Acquired Immune Deficiency Syndromes: JAIDS
https://www.readbyqxmd.com/read/29628508/a-pilot-study-of-ultra-deep-targeted-sequencing-of-plasma-dna-identifies-driver-mutations-in-hepatocellular-carcinoma
#12
Ismail Labgaa, Carlos Villacorta-Martin, Delia D'Avola, Amanda J Craig, Johann von Felden, Sebastiao N Martins-Filho, Daniela Sia, Ashley Stueck, Stephen C Ward, M Isabel Fiel, Milind Mahajan, Parissa Tabrizian, Swan N Thung, Celina Ang, Scott L Friedman, Josep M Llovet, Myron Schwartz, Augusto Villanueva
Cellular components of solid tumors including DNA are released into the bloodstream, but data on circulating-free DNA (cfDNA) in hepatocellular carcinoma (HCC) are still scarce. This study aimed at analyzing mutations in cfDNA and their correlation with tissue mutations in patients with HCC. We included 8 HCC patients treated with surgical resection for whom we collected paired tissue and plasma/serum samples. We analyzed 45 specimens, including multiregional tumor tissue sampling (n = 24), peripheral blood mononuclear cells (PMBC, n = 8), plasma (n = 8) and serum (n = 5)...
April 9, 2018: Oncogene
https://www.readbyqxmd.com/read/29627879/significance-of-circulating-epstein-barr-virus-dna-monitoring-after-remission-in-patients-with-extranodal-natural-killer-t-cell-lymphoma
#13
Jinhyun Cho, Seok Jin Kim, Silvia Park, Kwai Han Yoo, Chang-Seok Ki, Younghyeh Ko, Won Seog Kim
Circulating Epstein-Barr virus (EBV)-DNA has been established as a useful parameter for diagnosis and predicting prognosis in patients with extranodal natural killer T cell lymphoma (ENKTL); however, the role of monitoring of circulating EBV-DNA after complete remission (CR) is not well established. From January 2008 to August 2016, 328 ENKTL patents were enrolled in 2 lymphoma cohorts. Of 171 patients achieved a CR, 81 had available monitoring data for circulating EBV-DNA with negative post-treatment EBV-DNA...
April 8, 2018: Annals of Hematology
https://www.readbyqxmd.com/read/29627453/role-of-circulating-tumor-dna-in-the-management-of-patients-with-colorectal-cancer
#14
Emilie Moati, Valérie Taly, Audrey Didelot, Géraldine Perkins, Hélène Blons, Julien Taieb, Pierre Laurent-Puig, Aziz Zaanan
Colorectal cancer is a major health burden with a prognosis that has been improved with the progresses in diagnosis and the advance of chemotherapy and personalized medicine. However, because of intra-tumor heterogeneity, clonal evolution and selection, tumors often develop resistance to treatments. "Liquid biopsy" is a minimally invasive method, based on analysis of tumor-specific material in peripheral blood samples of patients. Analysis of tumor specific genetic or epigenetic alterations in cell-free circulating nucleic acids may reflect the molecular heterogeneity of the underlying disease process and serial testing could allow to monitor its temporal genomic changing without using re-biopsy...
April 4, 2018: Clinics and Research in Hepatology and Gastroenterology
https://www.readbyqxmd.com/read/29627389/plasma-msept9-a-novel-circulating-cell-free-dna-based-epigenetic-biomarker-to-diagnose-hepatocellular-carcinoma
#15
Abderrahim Oussalah, Susann Rischer, Mouni Bensenane, Guillaume Conroy, Pierre Filhine-Tresarrieu, Renée Debard, Denise Forest-Tramoy, Thomas Josse, Dana Reinicke, Matthieu Garcia, Amandine Luc, Cédric Baumann, Ahmet Ayav, Valérie Laurent, Marcus Hollenbach, Cristina Ripoll, Rosa-Maria Guéant-Rodriguez, Fares Namour, Alexander Zipprich, Michael Fleischhacker, Jean-Pierre Bronowicki, Jean-Louis Guéant
BACKGROUND: Patients with cirrhosis are at high risk of hepatocellular carcinoma (HCC). The SEPT9 gene is a key regulator of cell division and tumor suppressor whose hypermethylation is associated with liver carcinogenesis. The primary aim of this study was to evaluate the diagnostic accuracy of a PCR-based assay for the analysis of SEPT9 promoter methylation in circulating cell-free DNA (mSEPT9) for diagnosing HCC among cirrhotic patients. METHODS: We report two phase II biomarker studies that included cirrhotic patients with or without HCC from France (initial study) and Germany (replication study)...
March 28, 2018: EBioMedicine
https://www.readbyqxmd.com/read/29626711/non-invasive-prenatal-diagnosis-of-fetal-aneuploidy-using-cell-free-fetal-dna
#16
REVIEW
Barbara Renga
The discovery of cell free fetal DNA in maternal plasma has stimulated a rapid development of non-invasive prenatal testing. The recent advent of massively parallel sequencing has allowed the analysis of circulating cell-free fetal DNA to be performed with unprecedented sensitivity and precision. It is thus expected that plasma DNA-based non invasive prenatal testing will play an increasingly important role in the future of obstetric care. The present review summarizes recent advances in non invasive prenatal testing using cell free fetal DNA...
March 20, 2018: European Journal of Obstetrics, Gynecology, and Reproductive Biology
https://www.readbyqxmd.com/read/29626548/clinical-indications-for-and-the-future-of-circulating-tumor-cells
#17
Dominic H Moon, Daniel P Lindsay, Seungpyo Hong, Andrew Z Wang
Circulating tumor cells (CTCs) are cells that have detached from the primary tumor and entered circulation with potential to initiate a site of metastasis. Currently, CTC detection using CellSearch is cleared by the Food and Drug Administration for monitoring metastatic breast, prostate, and colorectal cancers as a prognostic biomarker for progression-free and overall survival. Accumulating evidence suggests CTCs have similar prognostic value in other metastatic and non-metastatic settings. Current research efforts are focused on extending the utility of CTCs beyond a prognostic biomarker to help guide clinical decision-making...
April 4, 2018: Advanced Drug Delivery Reviews
https://www.readbyqxmd.com/read/29625922/new-records-of-tick-associated-spotted-fever-group-rickettsia-in-an-amazon-savannah-ecotone-brazil
#18
A A R Aguirre, Marcos Valério Garcia, Ivaneide Nunes da Costa, Bárbara Guimarães Csordas, Vinícius da Silva Rodrigues, Jansen Fernandes Medeiros, Renato Andreotti
Human rickettsiosis has been recorded in the Amazon Biome. However, the epidemiological cycle of causative rickettsiae has not been fully accounted for in the Amazon region. This study investigates the presence of spotted fever group (SFG) Rickettsia spp. in free-living unfed ticks of the Amblyomma genus. The study was conducted in seven municipalities in Rondonia State, Brazil, where the main biomes are Amazon forest, Brazilian Savannah and their ecotones (areas of ecological tension between open ombrophilous forest and savannah)...
March 22, 2018: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/29625863/circulating-cell-free-dna-for-non-invasive-cancer-management
#19
REVIEW
Caitlin M Stewart, Dana W Y Tsui
Cell-free DNA (cfDNA) was first identified in human plasma in 1948 and is thought to be released from cells throughout the body into the circulatory system. In cancer, a portion of the cfDNA originates from tumour cells, referred to as circulating-tumour DNA (ctDNA), and can contain mutations corresponding to the patient's tumour, for instance specific TP53 alleles. Profiling of cfDNA has recently become an area of increasing clinical relevance in oncology, in particular due to advances in the sensitivity of molecular biology techniques and development of next generation sequencing technologies, as this allows tumour mutations to be identified and tracked non-invasively...
March 11, 2018: Cancer Genetics
https://www.readbyqxmd.com/read/29623586/comparison-of-plasma-ctdna-and-tissue-cytology-based-techniques-for-the-detection-of-egfr-mutation-status-in-advanced-nsclc-spanish-data-subset-from-assess
#20
E Arriola, A Paredes-Lario, R García-Gomez, P Diz-Tain, M Constenla, C García-Girón, G Márquez, M Reck, G López-Vivanco
PURPOSE: The analysis of epidermal growth factor receptor (EGFR) mutations in many patients with advanced non-small-cell lung cancer (aNSCLC) has provided the opportunity for successful treatment with specific, targeted EGFR tyrosine kinase inhibitors. However, this therapeutic decision may be challenging when insufficient tumor tissue is available for EGFR mutation testing. Therefore, blood surrogate samples for EGFR mutation analysis have been suggested. METHODS: Data were collected from the Spanish cohort of patients in the large, non-interventional, diagnostic ASSESS study (NCT01785888) evaluating the utility of circulating free tumor-derived DNA from plasma for EGFR mutation testing...
April 5, 2018: Clinical & Translational Oncology
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