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Junqiang Zhang, Xianbin Song, Wei Cao, Jinseng Lu, Xiaoqing Wang, Gaoyuan Wang, Zhicheng Wang, Xiaoyu Chen
Resveratrol is a polyphenol derivatives which exhibits a pro-apoptotic effect in a variety of human cancers by triggering mitochondria apoptosis pathway and autophagy. However, there are scarcely reports on its apoptosis-promoting effect in abnormal proliferation fibroblast-like synoviocytes (FLSs). In this study, we investigated the underlying mechanism and apoptosis-inducing effects of resveratrol on the abnormal proliferation of FLSs in adjuvant-arthritis (AA) rats. Since using resveratrol for 12 days resulted in a significant decreasing the swelling degree of the paw, reducing malondialdehyde (MDA) content and enhancing superoxide dismutase (SOD) activity, antioxidant capacity, glutathione peroxidase and glutathione reductase ratio in AA rats...
2016: Scientific Reports
Md Razia Sultana, Pankaj K Bagul, Parameshwar B Katare, Soheb Anwar, Raju Padiya, Sanjay K Banerjee
BACKGROUND: Cardiac complications are major contributor in the mortality of diabetic people. Mitochondrial dysfunctioning is a crucial contributor for the cardiac complications in diabetes, and SIRT-3 remains the major mitochondrial deacetylase. We hypothesized whether garlic has any role on SIRT-3 to prevent mitochondrial dysfunction in diabetic heart. METHODS: Rats with developed hyperglycemia after STZ injection were divided into two groups; diabetic (Dia) and diabetic+garlic (Dia+Garl)...
August 30, 2016: Life Sciences
Sneha Sagarkar, Deepa Gandhi, S Saravana Devi, Amul Sakharkar, Atya Kapley
OBJECTIVE: Chronic exposure to atrazine and other pesticides is reported to cause metabolic disorders, yet information on effects of atrazine on expression of genes relevant to mitochondrial function is largely missing. In the present study, therefore, we investigated the expression of a battery of nuclear- and mitochondrial-encoded genes involved in oxidative phosphorylation (OXPHOS) in human liver (HepG2) and rat muscle (L6) cell lines due to short-term atrazine exposure. MATERIALS AND METHODS: We have determined the EC50 values of atrazine for cytotoxicity and mitochondrial toxicity (mitotoxicity) in terms of adenosine triphosphate (ATP) content in HepG2 and L6 cells...
March 2016: Indian Journal of Pharmacology
Yu-Xuan Luo, Xiaoqiang Tang, Xi-Zhou An, Xue-Min Xie, Xiao-Feng Chen, Xiang Zhao, De-Long Hao, Hou-Zao Chen, De-Pei Liu
AIMS: Oxidative stress contributes to the development of cardiac hypertrophy and heart failure. One of the mitochondrial sirtuins, Sirt4, is highly expressed in the heart, but its function remains unknown. The aim of the present study was to investigate the role of Sirt4 in the pathogenesis of pathological cardiac hypertrophy and the molecular mechanism by which Sirt4 regulates mitochondrial oxidative stress. METHODS AND RESULTS: Male C57BL/6 Sirt4 knockout mice, transgenic (Tg) mice exhibiting cardiac-specific overexpression of Sirt4 (Sirt4-Tg) and their respective controls were treated with angiotensin II (Ang II, 1...
April 20, 2016: European Heart Journal
Jing-Yi Zhang, Yong-Ning Deng, Meng Zhang, Hua Su, Qiu-Min Qu
SIRT3 is a member of Sirtuins family, which belongs to NAD(+) dependent class III histone deacetylases. Emerging evidence suggests that SIRT3 plays a pivotal role in regulating mitochondrial function. Mitochondrial dysfunction is a main pathogenesis of Parkinson's disease (PD). Here, we have investigated the protective effect of SIRT3 for PD cell model. The rotenone-induced human neuroblastoma SH-SY5Y cells damage was used as PD cell model. The lentiviral vectors were used to over-expression or knockdown SIRT3 expression...
July 2016: Neurochemical Research
I Marques-Aleixo, E Santos-Alves, M M Balça, P I Moreira, P J Oliveira, J Magalhães, A Ascensão
Doxorubicin (DOX) is a highly effective anti-neoplastic agent, whose clinical use is limited by a dose-dependent mitochondrial toxicity in non-target tissues, including the brain. Here we analyzed the effects of distinct exercise modalities (12-week endurance treadmill-TM or voluntary free-wheel activity-FW) performed before and during sub-chronic DOX treatment on brain cortex and cerebellum mitochondrial bioenergetics, oxidative stress, permeability transition pore (mPTP), and proteins involved in mitochondrial biogenesis, apoptosis and auto(mito)phagy...
January 2016: Mitochondrion
Lichang Zhao, Hui Zhang, Jingfang Bao, Jun Liu, Zhongning Ji
Saikosaponin-d (Ssd) is one of the major pharmacologically active molecules present in Bupleurum falcatum L, a medical herb against inflammatory diseases in the traditional Chinese medicine. In the current study, we investigated the protective activity of Ssd on diabetic nephropathy along with the underlying mechanisms using renal tubular epithelial cell line (NRK-52E). Our study showed that high glucose stimulation significantly increased NRK-52E cell proliferation. Ssd administration dramatically inhibited high glucose-induced proliferation and DNA synthesis in NRK-52E cell...
2015: International Journal of Clinical and Experimental Medicine
I Marques-Aleixo, E Santos-Alves, M M Balça, D Rizo-Roca, P I Moreira, P J Oliveira, J Magalhães, A Ascensão
We here investigate the effects of two exercise modalities (endurance treadmill training-TM and voluntary free-wheel activity-FW) on the brain cortex and cerebellum mitochondrial bioenergetics, permeability transition pore (mPTP), oxidative stress, as well as on proteins involved in mitochondrial biogenesis, apoptosis, and quality control. Eighteen male rats were assigned to sedentary-SED, TM and FW groups. Behavioral alterations and ex vivo brain mitochondrial function endpoints were assessed. Proteins involved in oxidative phosphorylation (OXPHOS, including the adenine nucleotide translocator), oxidative stress markers and regulatory proteins (SIRT3, p66shc, UCP2, carbonyls, MDA, -SH, aconitase, Mn-SOD), as well as proteins involved in mitochondrial biogenesis (PGC1α, TFAM) were evaluated...
August 20, 2015: Neuroscience
Kuo-Yuan Cheng, Fei Guo, Jia-Qi Lu, Yuan-Zhao Cao, Tian-Chang Wang, Qi Yang, Qing Xia
AIMS: Oxidative stress is a direct cause of injury in various neural diseases. Manganese porphyrins (MnPs), a large category of superoxide dismutase (SOD) mimics, shown universally to have effects in numerous neural disease models in vivo. Given their complex intracellular redox activities, detailed mechanisms underlying the biomedical efficacies are not fully elucidated. This study sought to investigate the regulation of endogenous antioxidant systems by a MnP (MnTM-4-PyP) and its role in the protection against neural oxidative stress...
May 2015: CNS Neuroscience & Therapeutics
Inês Marques-Aleixo, Estela Santos-Alves, Diogo Mariani, David Rizo-Roca, Ana I Padrão, Sílvia Rocha-Rodrigues, Ginés Viscor, J Ramon Torrella, Rita Ferreira, Paulo J Oliveira, José Magalhães, António Ascensão
Doxorubicin (DOX) is an anti-cancer agent whose clinical usage results in a cumulative and dose-dependent cardiotoxicity. We have previously shown that exercise performed prior to DOX treatment reduces the resulting cardiac(mito) toxicity. We sought to determine the effects on cardiac mitochondrial toxicity of two distinct chronic exercise models (endurance treadmill training-TM and voluntary free-wheel activity-FW) when used prior and during DOX treatment. Male-young Sprague-Dawley rats were divided into six groups (n=6 per group): SAL+SED (saline sedentary), SAL+TM (12-weeks TM), SAL+FW (12-weeks FW), DOX+SED (7-weeks of chronic DOX treatment 2mg/kg per week), DOX+TM and DOX+FW...
January 2015: Mitochondrion
Eun-Seok Park, Jun Chul Kang, Yong Chang Jang, Jong Seok Park, Shin Yi Jang, Dae-Eun Kim, Bokyung Kim, Hwa-Sup Shin
ETHNOPHARMACOLOGICAL RELEVANCE: Many studies have emphasized that flavonoids, found in various fruits, vegetables, and seeds, as well as tea and red wine, have potential health-promoting and disease-preventing effects. Rhamnetin is a flavonoid that exhibits antioxidant capabilities. However, little is known about its effect on cardiac myocytes under oxidative stress and the underlying mechanisms. MATERIALS AND METHODS: H9c2 cardiomyoblast cells were subjected to H2O2, to study the protective effect of rhamnetin on cell viability, apoptosis, and ROS production...
May 14, 2014: Journal of Ethnopharmacology
Luena Papa, Mary Hahn, Ellen L Marsh, Bradley S Evans, Doris Germain
Cancer cells are characterized by elevated levels of reactive oxygen species, which are produced mainly by the mitochondria. The dismutase SOD2 localizes in the matrix and is a major antioxidant. The activity of SOD2 is regulated by the deacetylase SIRT3. Recent studies indicated that SIRT3 is decreased in 87% of breast cancers, implying that the activity of SOD2 is compromised. The resulting elevation in reactive oxygen species was shown to be essential for the metabolic reprograming toward glycolysis. Here, we show that SOD2 itself is down-regulated in breast cancer cell lines...
February 28, 2014: Journal of Biological Chemistry
Carolyn M Roos, Michael Hagler, Bin Zhang, Elise A Oehler, Arman Arghami, Jordan D Miller
The purpose of this study was to characterize changes in antioxidant and age-related gene expression in aorta and aortic valve with aging, and test the hypothesis that increased mitochondrial oxidative stress accelerates age-related endothelial and aortic valve dysfunction. Wild-type (MnSOD(+/+)) and manganese SOD heterozygous haploinsufficient (MnSOD(+/-)) mice were studied at 3 and 18 mo of age. In aorta from wild-type mice, antioxidant expression was preserved, although there were age-associated increases in Nox2 expression...
November 15, 2013: American Journal of Physiology. Heart and Circulatory Physiology
Jian-mei Gao, Ran Li, Lei Zhang, Li-long Jia, Xi-xiang Ying, De-qiang Dou, Jian-chun Li, Hai-bo Li
ETHNOPHARMACOLOGICAL RELEVANCE: Cuscuta chinensis (C. chinensis) is a well-known traditional Chinese herb that has been used to treat heart disease, diabetes, liver injury, cancer, and aging. MATERIALS AND METHODS: Murine osteoblastic MC3T3-E1 cells were treated with various concentrations of C. chinensis water extraction at different time intervals. The antioxidant effect of C. chinensis on MC3T3-E1 cells was evaluated using MTT and TUNEL assays. The effect of C...
July 9, 2013: Journal of Ethnopharmacology
Zsolt Radak, Zoltan Bori, Erika Koltai, Ioannis G Fatouros, Athanasios Z Jamurtas, Ioannis I Douroudos, Gerasimos Terzis, Michalis G Nikolaidis, Athanasios Chatzinikolaou, Apostolos Sovatzidis, Shuzo Kumagai, Hisahi Naito, Istvan Boldogh
8-Oxo-7,8-dihydroguanine (8-oxoG) accumulates in the genome over time and is believed to contribute to the development of aging characteristics of skeletal muscle and various aging-related diseases. Here, we show a significantly increased level of intrahelical 8-oxoG and 8-oxoguanine-DNA glycosylase (OGG1) expression in aged human skeletal muscle compared to that of young individuals. In response to exercise, the 8-oxoG level was lastingly elevated in sedentary young and old subjects, but returned rapidly to preexercise levels in the DNA of physically active individuals independent of age...
July 15, 2011: Free Radical Biology & Medicine
Gabriella Marfe, Marco Tafani, Bruna Pucci, Carla Di Stefano, Manuela Indelicato, Angela Andreoli, Matteo Antonio Russo, Paola Sinibaldi-Salimei, Vincenzo Manzi
BACKGROUND: A large body of evidence shows that a single bout of strenuous exercise induces oxidative stress in circulating human lymphocytes leading to lipid peroxidation, DNA damage, mitochondrial perturbations, and protein oxidation.In our research, we investigated the effect of physical load on the extent of apoptosis in primary cells derived from blood samples of sixteen healthy amateur runners after marathon (a.m.). RESULTS: Blood samples were collected from ten healthy amateur runners peripheral blood mononuclear cells (PBMCs) were isolated from whole blood and bcl-2, bax, heat shock protein (HSP)70, Cu-Zn superoxide dismutase (SOD), Mn-SOD, inducible nitric oxide synthase (i-NOS), SIRT1, SIRT3 and SIRT4 (Sirtuins) RNA levels were determined by Northern Blot analysis...
2010: BMC Physiology
Gabriella Marfe, Marco Tafani, Manuela Indelicato, Paola Sinibaldi-Salimei, Valentina Reali, Bruna Pucci, Massimo Fini, Matteo Antonio Russo
Kaempferol (3,4',5,7-tetrahydroxyflavone) is a flavonoid with anti- and pro-oxidant activity present in various natural sources. Kaempferol has been shown to posses anticancer properties through the induction of the apoptotic program. Here we report that treatment of the chronic myelogenous leukemia cell line K562 and promyelocitic human leukemia U937 with 50 microM kaempferol resulted in an increase of the antioxidant enzymes Mn and Cu/Zn superoxide dismutase (SOD). Kaempferol treatment induced apoptosis by decreasing the expression of Bcl-2 and increasing the expressions of Bax...
March 1, 2009: Journal of Cellular Biochemistry
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