Amrita Salvi, Alexandria N Young, Andrew C Huntsman, Melissa R Pergande, Melissa A Korkmaz, Rathnayake A Rathnayake, Brittney K Mize, A Douglas Kinghorn, Xiaoli Zhang, Kiira Ratia, Markus Schirle, Jason R Thomas, Scott M Brittain, Claude Shelton, Leslie N Aldrich, Stephanie M Cologna, James R Fuchs, Joanna E Burdette
PHY34 is a synthetic small molecule, inspired by a compound naturally occurring in tropical plants of the Phyllanthus genus. PHY34 was developed to have potent in vitro and in vivo anticancer activity against high grade serous ovarian cancer (HGSOC) cells. Mechanistically, PHY34 induced apoptosis in ovarian cancer cells by late-stage autophagy inhibition. Furthermore, PHY34 significantly reduced tumor burden in a xenograft model of ovarian cancer. In order to identify its molecular target/s, we undertook an unbiased approach utilizing mass spectrometry-based chemoproteomics...
January 10, 2022: Cell Death & Disease