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https://www.readbyqxmd.com/read/29131418/stereoselective-nanozyme-based-on-ceria-nanoparticles-engineered-with-amino-acids
#1
Xiaogang Qu, Yuhuan Sun, Chuanqi Zhao, Nan Gao, Jinsong Ren
Stereoselectivity towards substrate is one of the most important characteristics of enzymes. Amino acids, as cofactors of many enzymes, play important roles in stereochemistry. Herein, chiral nanozymes were constructed by grafting a series of D- or L-amino acids on surfaces of ceria (cerium oxide) nanoparticles. We selected the most commonly used drug for combating Parkinson's disease 3, 4-dihydroxyphenylalanine (DOPA) enantiomers as examples for chiral catalysis. Through detailed kinetic studies of eight amino acids modified cerium oxide nanoparticles (CeNP), we found that phenylalanine-modified CeNP was optimal for the DOPA oxidation reaction and showed excellent stereoselectivity towards its enantiomers...
November 12, 2017: Chemistry: a European Journal
https://www.readbyqxmd.com/read/29119119/an-exploration-of-the-impact-of-anticentromere-antibody-on-early-stage-embryo
#2
Ying Ying, Xi Guo, Yiping Zhong, Canquan Zhou
Background: Previously, we found women with positive anticentromere antibody showed impaired potential of oocyte maturation and embryo cleavage; the possible mechanism behind this phenomenon was still unknown. Objective: Thus, the present study aimed to preliminarily explore whether ACA could penetrate into the living embryos and impair their developmental potential via in vitro coculture with mouse embryos. Methods: Mouse embryos were collected and used for in vitro culture with polyclonal anticentromere protein A (CENP-A) antibody; then, immunofluorescence assay was performed to determine the penetration of antibody into embryos, and embryo development potential was observed...
2017: Journal of Immunology Research
https://www.readbyqxmd.com/read/29074736/molecular-basis-of-cenp-c-association-with-the-cenp-a-nucleosome-at-yeast-centromeres
#3
Hua Xiao, Feng Wang, Jan Wisniewski, Alexey K Shaytan, Rodolfo Ghirlando, Peter C FitzGerald, Yingzi Huang, Debbie Wei, Shipeng Li, David Landsman, Anna R Panchenko, Carl Wu
Histone CENP-A-containing nucleosomes play an important role in nucleating kinetochores at centromeres for chromosome segregation. However, the molecular mechanisms by which CENP-A nucleosomes engage with kinetochore proteins are not well understood. Here, we report the finding of a new function for the budding yeast Cse4/CENP-A histone-fold domain interacting with inner kinetochore protein Mif2/CENP-C. Strikingly, we also discovered that AT-rich centromere DNA has an important role for Mif2 recruitment. Mif2 contacts one side of the nucleosome dyad, engaging with both Cse4 residues and AT-rich nucleosomal DNA...
October 26, 2017: Genes & Development
https://www.readbyqxmd.com/read/29061732/sld5-ensures-centrosomal-resistance-to-congression-forces-by-preserving-centriolar-satellites
#4
Manpreet Kaur, Raksha Devi, Tanushree Ghosh, Md Muntaz Khan, Praveen Kumar, Priyanka, Ananya Kar, Aparna Sharma, Akhil Varshney, Vipin Kumar, Sandeep Saxena
The migration of chromosomes during mitosis is mediated primarily by kinesins that bind to the chromosomes and move along the microtubules, exerting pulling and pushing forces on the centrosomes. We report that a DNA replication protein, Sld5, localizes to the centrosomes resisting the microtubular pulling forces experienced during chromosome congression. In the absence of Sld5, centriolar satellites, which normally cluster around the centrosomes, are dissipated throughout the cytoplasm, resulting in the loss of their known function of recruiting the centrosomal protein, pericentrin...
October 23, 2017: Molecular and Cellular Biology
https://www.readbyqxmd.com/read/29046335/molecular-basis-for-inner-kinetochore-configuration-through-rwd-domain-peptide-interactions
#5
Florian Schmitzberger, Magdalena M Richter, Yuliya Gordiyenko, Carol V Robinson, Michał Dadlez, Stefan Westermann
Kinetochores are dynamic cellular structures that connect chromosomes to microtubules. They form from multi-protein assemblies that are evolutionarily conserved between yeasts and humans. One of these assemblies-COMA-consists of subunits Ame1(CENP-U), Ctf19(CENP-P), Mcm21(CENP-O) and Okp1(CENP-Q) A description of COMA molecular organization has so far been missing. We defined the subunit topology of COMA, bound with inner kinetochore proteins Nkp1 and Nkp2, from the yeast Kluyveromyces lactis, with nanoflow electrospray ionization mass spectrometry, and mapped intermolecular contacts with hydrogen-deuterium exchange coupled to mass spectrometry...
October 18, 2017: EMBO Journal
https://www.readbyqxmd.com/read/29040671/nanoscale-dynamics-of-centromere-nucleosomes-and-the-critical-roles-of-cenp-a
#6
Micah P Stumme-Diers, Siddhartha Banerjee, Mohtadin Hashemi, Zhiqiang Sun, Yuri L Lyubchenko
In the absence of a functioning centromere, chromosome segregation becomes aberrant, leading to an increased rate of aneuploidy. The highly specific recognition of centromeres by kinetochores suggests that specific structural characteristics define this region, however, the structural details and mechanism underlying this recognition remains a matter of intense investigation. To address this, high-speed atomic force microscopy was used for direct visualization of the spontaneous dynamics of CENP-A nucleosomes at the sub-second time scale...
October 10, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/28980868/exploring-the-role-of-cenp-a-ser18-phosphorylation-in-cin-and-tumorigenesis
#7
Weiguo Zhang, Gary H Karpen, Qing Zhang
No abstract text is available yet for this article.
October 5, 2017: Cell Cycle
https://www.readbyqxmd.com/read/28980861/budding-yeast-cenp-a-cse4-interacts-with-the-n-terminus-of-sgo1-and-regulates-its-association-with-centromeric-chromatin
#8
Prashant K Mishra, Kriti S Thapa, Panyue Chen, Suyu Wang, Tony R Hazbun, Munira A Basrai
Shugoshin is an evolutionarily conserved protein, which is involved in tension sensing on mitotic chromosomes, kinetochore biorientation, and protection of centromeric (CEN) cohesin for faithful chromosome segregation. Interaction of the C-terminus of Sgo1 with phosphorylated histone H2A regulates its association with CEN and pericentromeric (peri-CEN) chromatin, whereas mutations in histone H3 selectively compromise the association of Sgo1 with peri-CEN but not CEN chromatin. Given that histone H3 is absent from CEN and is replaced by a histone H3 variant CENP-A(Cse4), we investigated if CENP-A(Cse4) interacts with Sgo1 and promotes its association with the CEN chromatin...
October 5, 2017: Cell Cycle
https://www.readbyqxmd.com/read/28977935/overexpression-of-the-e2f-target-gene-cenpi-promotes-chromosome-instability-and-predicts-poor-prognosis-in-estrogen-receptor-positive-breast-cancer
#9
Pulari U Thangavelu, Cheng-Yu Lin, Srividya Vaidyanathan, Thu H M Nguyen, Eloise Dray, Pascal H G Duijf
During cell division, chromosome segregation is facilitated by the mitotic checkpoint, or spindle assembly checkpoint (SAC), which ensures correct kinetochore-microtubule attachments and prevents premature sister-chromatid separation. It is well established that misexpression of SAC components on the outer kinetochores promotes chromosome instability (CIN) and tumorigenesis. Here, we study the expression of CENP-I, a key component of the HIKM complex at the inner kinetochores, in breast cancer, including ductal, lobular, medullary and male breast carcinomas...
September 22, 2017: Oncotarget
https://www.readbyqxmd.com/read/28977643/regulation-of-mitotic-recombination-between-dna-repeats-in-centromeres
#10
Faria Zafar, Akiko K Okita, Atsushi T Onaka, Jie Su, Yasuhiro Katahira, Jun-Ichi Nakayama, Tatsuro S Takahashi, Hisao Masukata, Takuro Nakagawa
Centromeres that are essential for faithful segregation of chromosomes consist of unique DNA repeats in many eukaryotes. Although recombination is under-represented around centromeres during meiosis, little is known about recombination between centromere repeats in mitotic cells. Here, we compared spontaneous recombination that occurs between ade6B/ade6X inverted repeats integrated at centromere 1 (cen1) or at a non-centromeric ura4 locus in fission yeast. Remarkably, distinct mechanisms of homologous recombination (HR) were observed in centromere and non-centromere regions...
August 29, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/28974540/the-fission-yeast-nucleoporin-alm1-is-required-for-proteasomal-degradation-of-kinetochore-components
#11
Silvia Salas-Pino, Paola Gallardo, Ramón R Barrales, Sigurd Braun, Rafael R Daga
Kinetochores (KTs) are large multiprotein complexes that constitute the interface between centromeric chromatin and the mitotic spindle during chromosome segregation. In spite of their essential role, little is known about how centromeres and KTs are assembled and how their precise stoichiometry is regulated. In this study, we show that the nuclear pore basket component Alm1 is required to maintain both the proteasome and its anchor, Cut8, at the nuclear envelope, which in turn regulates proteostasis of certain inner KT components...
November 6, 2017: Journal of Cell Biology
https://www.readbyqxmd.com/read/28957864/meet-ancc-s-new-executive-leaders
#12
Loressa Cole, Jeffrey N Doucette
In October 2016, the American Nurses Credentialing Center (ANCC) announced the appointment of Loressa Cole, DNP, MBA, RN, FACHE, NEA-BC, as its new executive director and executive vice president and Jeffrey Doucette, DNP, RN, CENP, FACHE, NEA-BC, LNHA, as its new vice president of the Magnet Recognition® and Pathway to Excellence® Programs. In this Magnet® Perspectives column, we learn more about these 2 dynamic leaders at the helm of ANCC.
October 2017: Journal of Nursing Administration
https://www.readbyqxmd.com/read/28951328/autoantibodies-of-igm-and-igg-classes-show-differences-in-recognition-of-multiple-autoantigens-in-chronic-obstructive-pulmonary-disease
#13
Reham Shindi, Amna Almehairi, Ola H Negm, Noor Kalsheker, Nichola S Gale, Dennis J Shale, Timothy W Harrison, Charlotte E Bolton, Michelle John, Ian Todd, Patrick J Tighe, Lucy C Fairclough
Autoimmunity occurs in chronic obstructive pulmonary disease (COPD). We describe an antigen microarray for detecting serum autoantibodies (AAbs) to determine how IgM, as well as IgG, AAbs distinguish patients with COPD from controls with a history of smoking without COPD. All COPD patients' sera contained elevated levels of AAbs to some of 30 autoantigens. There were significant differences in the autoantigenic specificities of IgM AAbs compared to IgG AAbs in the COPD sera: for example, AAbs to histone and scl-70 were mainly IgG, whereas AAbs to CENP-B and La/ssB were mainly IgM; by contrast, IgM and IgG AAbs to collagen-V were equally prevalent...
October 2017: Clinical Immunology: the Official Journal of the Clinical Immunology Society
https://www.readbyqxmd.com/read/28940121/anti-cenp-b-and-anti-topo-1-containing-sera-from-systemic-sclerosis-related-diseases-with-raynaud-s-phenomenon-induce-vascular-endothelial-cell-senescence-not-via-classical-p53-p21-pathway
#14
Chieh-Yu Shen, Ko-Jen Li, Pei-Hsuan Lai, Chia-Li Yu, Song-Chou Hsieh
Raynaud's phenomenon (RP) is the earliest and most common clinical manifestation in patients with systemic sclerosis (SSc) and its related diseases containing anti-TOPO-1 and/or anti-CENP-B autoantibodies in the sera. However, the cause-effect relationship between the two autoantibodies and RP remains elucidation. Sera containing anti-CENP-B and anti-TOPO-1 autoantibodies were obtained from SSc-related diseases manifesting RP. The polyclonal auto-antibodies were purified from pooled sera by affinity chromatography...
September 23, 2017: Clinical Rheumatology
https://www.readbyqxmd.com/read/28920929/structural-investigation-of-nucleophosmin-interaction-with-the-tumor-suppressor-fbw7%C3%AE
#15
A Di Matteo, M Franceschini, A Paiardini, A Grottesi, S Chiarella, S Rocchio, C Di Natale, D Marasco, L Vitagliano, C Travaglini-Allocatelli, L Federici
Nucleophosmin (NPM1) is a multifunctional nucleolar protein implicated in ribogenesis, centrosome duplication, cell cycle control, regulation of DNA repair and apoptotic response to stress stimuli. The majority of these functions are played through the interactions with a variety of protein partners. NPM1 is frequently overexpressed in solid tumors of different histological origin. Furthermore NPM1 is the most frequently mutated protein in acute myeloid leukemia (AML) patients. Mutations map to the C-terminal domain and lead to the aberrant and stable localization of the protein in the cytoplasm of leukemic blasts...
September 18, 2017: Oncogenesis
https://www.readbyqxmd.com/read/28916652/lsd1-mediated-epigenetic-reprogramming-drives-cenpe-expression-and-prostate-cancer-progression
#16
Yi Liang, Musaddeque Ahmed, Haiyang Guo, Fraser Soares, Junjie T Hua, Shuai Gao, Catherine Lu, Christine Poon, Wanting Han, Jens Langstein, Muhammad B Ekram, Brian Li, Elai Davicioni, Mandeep Takhar, Nicholas Erho, R Jeffrey Karnes, Dianne Chadwick, Theodorus van der Kwast, Paul C Boutros, Cheryl H Arrowsmith, Felix Y Feng, Anthony M Joshua, Amina Zoubeidi, Changmeng Cai, Housheng H He
Androgen receptor (AR) signaling is a key driver of prostate cancer, and androgen-deprivation therapy (ADT) is a standard treatment for patients with advanced and metastatic disease. However, patients receiving ADT eventually develop incurable castration-resistant prostate cancer (CRPC). Here, we report that the chromatin modifier LSD1, an important regulator of AR transcriptional activity, undergoes epigenetic reprogramming in CRPC. LSD1 reprogramming in this setting activated a subset of cell-cycle genes, including CENPE, a centromere binding protein and mitotic kinesin...
October 15, 2017: Cancer Research
https://www.readbyqxmd.com/read/28904333/the-ino80-complex-mediates-epigenetic-centromere-propagation-via-active-removal-of-histone-h3
#17
Eun Shik Choi, Youngseo Cheon, Keunsoo Kang, Daeyoup Lee
The centromere is the chromosomal locus at which the kinetochore is assembled to direct chromosome segregation. The histone H3 variant, centromere protein A (CENP-A), is known to epigenetically mark active centromeres, but the mechanism by which CENP-A propagates at the centromere, replacing histone H3, remains poorly understood. Using fission yeast, here we show that the Ino80 adenosine triphosphate (ATP)-dependent chromatin-remodeling complex, which removes histone H3-containing nucleosomes from associated chromatin, promotes CENP-A(Cnp1) chromatin assembly at the centromere in a redundant manner with another chromatin-remodeling factor Chd1(Hrp1)...
September 13, 2017: Nature Communications
https://www.readbyqxmd.com/read/28857608/proteomic-alterations-of-fibroblasts-induced-by-ovarian-cancer-cells-reveal-potential-cancer-targets
#18
X Y Zhang, S S Hong, M Zhang, Q Q Cai, M X Zhang, C J Xu
The common spread pattern of ovarian cancer is peritoneal implantation. The growth of the shed ovarian cancer cells in the peritoneal cavity is closely related to the tumor microenvironment. Cancer-associated fibroblasts are vital in the tumor microenvironment. It is not clearly defined that the protein expression alters during the activating process of fibroblasts. This study detected the protein alterations in fibroblasts induced by ovarian cancer cells and explored the potential biological relevance through two-dimensional gel electrophoresis and mass spectrometry...
August 31, 2017: Neoplasma
https://www.readbyqxmd.com/read/28840245/centromere-dynamics-in-male-and-female-germ-cells
#19
Elaine M Dunleavy, Caitríona M Collins
In sexually reproducing organisms the germ line is the cellular lineage that gives rise to gametes. All germ cells originate from germline stem cells that divide asymmetrically to generate gonial pre-cursors, which are amplified in number by mitotic divisions, undergo meiosis and eventually differentiate into mature gametes (haploid eggs and sperm). Information transmitted with gametes is inherited by offspring, and potentially by subsequent generations, instructing in organismal development and beyond. Meiosis comprises one round of DNA replication, followed by two rounds of chromosome segregation; homologous chromosomes segregate in the first division (meiosis I) and sister chromatids segregate in the second division (meiosis II)...
2017: Progress in Molecular and Subcellular Biology
https://www.readbyqxmd.com/read/28840243/dna-sequences-in-centromere-formation-and-function
#20
M Dumont, D Fachinetti
Faithful chromosome segregation during cell division depends on the centromere, a complex DNA/protein structure that links chromosomes to spindle microtubules. This chromosomal domain has to be marked throughout cell division and its chromosomal localization preserved across cell generations. From fission yeast to human, centromeres are established on a series of repetitive DNA sequences and on specialized centromeric chromatin. This chromatin is enriched with the histone H3 variant, named CENP-A, that was demonstrated to be the epigenetic mark that maintains centromere identity and function indefinitely...
2017: Progress in Molecular and Subcellular Biology
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