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https://www.readbyqxmd.com/read/28330131/an-efficient-protocol-for-isolation-of-inhibitor-free-nucleic-acids-even-from-recalcitrant-plants
#1
Mohammad Hossein Rezadoost, Mojtaba Kordrostami, Hassan Hassani Kumleh
For fast and easy isolation of inhibitor-free genomic DNA even from the toughest plant leaf samples, including those high in polyphenols and polysaccharides, a protocol has been developed. To prevent the solubility of polysaccharides in the DNA extract, high salt concentration (1.4 M) was used in the extraction buffer. Polyvinylpyrrolidone (PVP) was used for the removal of polyphenols as polymerase chain reaction (PCR) inhibitors. Proteins like various enzymes were degraded by proteinase K and removed by centrifugation from plant extracts during the isolation process resulting in pure DNA and RNA ready to use in downstream applications including PCR, quantitative polymerase chain reaction (qPCR), ligation, restriction and sequencing...
June 2016: 3 Biotech
https://www.readbyqxmd.com/read/28329397/resveratrol-improves-vascular-function-and-mitochondrial-number-but-not-glucose-metabolism-in-older-adults
#2
Rena M Pollack, Nir Barzilai, Valentin Anghel, Ameya S Kulkarni, Aaron Golden, Pilib O'Broin, David A Sinclair, Michael S Bonkowski, Alexander J Coleville, Danielle Powell, Sharon Kim, Ruin Moaddel, Daniel Stein, Kehao Zhang, Meredith Hawkins, Jill P Crandall
Background: Resveratrol, a plant-derived polyphenol, has been reported to improve glucose metabolism and vascular function and to extend life span in animal models, but studies in humans have been inconclusive. Methods: In a randomized, double-blind crossover study, we treated older glucose-intolerant adults (n = 30) with resveratrol (2-3 g/daily) or placebo, each for 6 weeks. A standard mixed-meal test was used to assess insulin sensitivity (Matsuda index) and secretion (C-peptide deconvolution) and vascular function by reactive hyperemia peripheral arterial tonometry...
March 16, 2017: Journals of Gerontology. Series A, Biological Sciences and Medical Sciences
https://www.readbyqxmd.com/read/28329079/sleep-duration-and-cardiometabolic-risk-among-chinese-school-aged-children-do-adipokines-play-a-mediating-role
#3
Lujiao Li, Junling Fu, Xin Ting Yu, Ge Li, Lu Xu, Jinghua Yin, Hong Cheng, Dongqing Hou, Xiaoyuan Zhao, Shan Gao, Wenhui Li, Changhong Li, Struan F A Grant, Mingyao Li, Yi Xiao, Jie Mi, Ming Li
Objective: To assess the associations between sleep duration and cardiometabolic risk factors in Chinese school-aged children and to explore the possible mediating role of adipokines. Methods: Sleep duration was collected in 3,166 children from the Beijing Child and Adolescent Metabolic Syndrome study. Glucose homeostasis and other cardiometabolic risk factors were assessed. Serum adipokines including leptin, total and high-molecular-weight (HMW) adiponectin, resistin, fibroblast growth factor 21 (FGF21) and retinol binding protein 4 (RBP4) were determined...
March 9, 2017: Sleep
https://www.readbyqxmd.com/read/28328715/evaluating-injury-risk-and-gender-performance-on-health-and-skill-related-fitness-assessments
#4
Tyson L Grier, Michelle Canham-Chervak, Timothy T Bushman, Morgan K Anderson, William J North, Bruce H Jones
Grier, TL, Canham-Chervak, M, Bushman, TT, Anderson, MK, North, WJ, and Jones, BH. Evaluating injury risk and gender performance on health- and skill-related fitness assessments. J Strength Cond Res 31(4): 971-980, 2017-The purpose of this analysis was to investigate the association of injury risk and gender performance on health- and skill-related fitness assessments. A survey was used to collect personal characteristics and Army Physical Fitness Test scores (2-mile run, push-ups, and sit-ups). Within the same day, 9 physical fitness assessments were performed...
April 2017: Journal of Strength and Conditioning Research
https://www.readbyqxmd.com/read/28328503/fast-spin-echo-imaging-based-ept-with-k-space-weighting-via-t-sub-2-sub-relaxation-rept
#5
Jaewook Shin, Min-Oh Kim, Sungmin Cho, Dong-Hyun Kim
Magnetic resonance electrical property tomography (MREPT) is a technique used to extract the electrical properties of tissues (conductivity in particular) using a magnetic resonance imaging (MRI) system. In this work, we propose an improved data acquisition scheme for electrical property tomography technique by utilizing T2 modulation in fast spin echo (FSE) imaging. This technique was motivated by numerical analysis of conductivity reconstruction in the frequency domain; results reveal the spatial frequency-dependent noise texture of conventional methods...
March 17, 2017: IEEE Transactions on Medical Imaging
https://www.readbyqxmd.com/read/28327566/wholegrain-rye-but-not-wholegrain-wheat-lowers-body-weight-and-fat-mass-compared-with-refined-wheat-a-6-week-randomized-study
#6
J Suhr, S Vuholm, K N Iversen, R Landberg, M Kristensen
BACKGROUND: Observational studies suggest inverse associations between wholegrain intake and body weight gain. Only few controlled intervention studies have supported this association and few compare effects of different grain varieties. OBJECTIVE: To investigate how wholegrain wheat (WGW) and rye compared with refined wheat (RW) affect body weight and composition and appetite sensation. DESIGN: Seventy overweight/obese adults participated in this 6-week randomized parallel study, in which they replaced their habitual cereal foods with RW, WGW or wholegrain rye (WGR)...
March 22, 2017: European Journal of Clinical Nutrition
https://www.readbyqxmd.com/read/28327563/health-benefits-and-consequences-of-the-eastern-orthodox-fasting-in-monks-of-mount-athos-a-cross-sectional-study
#7
S N Karras, A Persynaki, A Petróczi, E Barkans, H Mulrooney, M Kypraiou, T Tzotzas, K Tziomalos, K Kotsa, A A Tsioudas, C Pichard, D P Naughton
BACKGROUND/OBJECTIVES: Greek Orthodox fasting (OF), which involves 180-200 days of fasting per year, is dictated by the Christian Orthodox religion. For the first time, this cross-sectional study examines the characteristics and the effects of OF on anthropometry, cardiometabolic markers and calcium homeostasis in Athonian monks (AMs). SUBJECTS/METHODS: Daily intakes of energy, macro- and micronutrients of a day during a weekend of Nativity Fast, defined as non-restrictive day (NRD), and a weekday during Great Lent, labeled as restrictive day (RD) were recorded...
March 22, 2017: European Journal of Clinical Nutrition
https://www.readbyqxmd.com/read/28327464/monte-carlo-simulations-of-time-of-flight-pet-with-double-ended-readout-calibration-coincidence-resolving-times-and-statistical-lower-bounds
#8
Stephen E Derenzo
This paper demonstrates through Monte Carlo simulations that a practical positron emission tomograph with (1) deep scintillators for efficient detection, (2) double-ended readout for depth-of-interaction information, (3) fixed-level analog triggering, and (4) accurate calibration and timing data corrections can achieve a coincidence resolving time (CRT) that is not far above the statistical lower bound. One Monte Carlo algorithm simulates a calibration procedure that uses data from a positron point source. Annihilation events with an interaction near the entrance surface of one scintillator are selected, and data from the two photodetectors on the other scintillator provide depth-dependent timing corrections...
March 22, 2017: Physics in Medicine and Biology
https://www.readbyqxmd.com/read/28327432/feedback-from-arctic-charr-feed-flavour-stimulation-and-re-feeding-after-feed-deprivation-stimulate-genes-encoding-both-orexigenic-and-anorexigenic-neuropeptides
#9
Anja Striberny, Even H Jørgensen
Despite vast research attention, the knowledge about central mechanisms of appetite regulation in teleost remains inconclusive. A common strategy in studies on appetite regulating mechanisms is to measure the response to feed restriction or - deprivation, but responses vary between fish species and between experiments, and are also likely dependent on the degree of energy perturbation. The anadromous Arctic charr is an interesting model for studying appetite regulation as its feeding cycle comprises months of winter anorexia, and hyperphagia during summer...
March 18, 2017: General and Comparative Endocrinology
https://www.readbyqxmd.com/read/28325852/extended-multiplexing-of-tmt-labeling-reveals-age-and-high-fat-diet-specific-proteome-changes-in-mouse-epididymal-adipose-tissue
#10
Deanna L Plubell, Phillip A Wilmarth, Yuqi Zhao, Alexandra M Fenton, Jessica Minnier, Ashok P Reddy, John Klimek, Xia Yang, Larry L David, Nathalie Pamir
The lack of high-throughput methods to analyze the adipose tissue protein composition limits our understanding of the protein networks responsible for age and diet related metabolic response. We have developed an approach using multiple-dimension liquid chromatography tandem mass spectrometry and extended multiplexing (24 biological samples) with TMT labeling to analyze proteomes of epididymal adipose tissues isolated from mice fed either low or high fat diet for a short or a long-term, and from mice that aged on low vs...
March 21, 2017: Molecular & Cellular Proteomics: MCP
https://www.readbyqxmd.com/read/28325448/diagnosis-of-autism-through-eeg-processed-by-advanced-computational-algorithms-a-pilot-study
#11
Enzo Grossi, Chiara Olivieri, Massimo Buscema
BACKGROUND: Multi-Scale Ranked Organizing Map coupled with Implicit Function as Squashing Time algorithm(MS-ROM/I-FAST) is a new, complex system based on Artificial Neural networks (ANNs) able to extract features of interest in computerized EEG through the analysis of few minutes of their EEG without any preliminary pre-processing. A proof of concept study previously published showed accuracy values ranging from 94%-98% in discerning subjects with Mild Cognitive Impairment and/or Alzheimer's Disease from healthy elderly people...
April 2017: Computer Methods and Programs in Biomedicine
https://www.readbyqxmd.com/read/28323999/metabolically-healthy-obesity-and-ischemic-heart-disease-a-10-year-follow-up-of-the-inter99-study
#12
Louise Hansen, Marie K Netterstrøm, Nanna B Johansen, Pernille F Rønn, Dorte Vistisen, Lise Ln Husemoen, Marit E Jørgensen, Naja H Rod, Kristine Færch
Context: Recent studies have suggested that a subgroup of obese individuals is not at increased risk of obesity-related complications. This subgroup has been referred to as metabolically healthy obese. Objective: To investigate whether obesity is a risk factor for development of ischemic heart disease (IHD) irrespective of metabolic health. Design: 6,238 men and women from the Danish prospective Inter99 study were followed during 10.6 (SD 1...
March 7, 2017: Journal of Clinical Endocrinology and Metabolism
https://www.readbyqxmd.com/read/28323972/neurosecretory-protein-gl-a-hypothalamic-small-secretory-protein-participates-in-energy-homeostasis-in-male-mice
#13
Daichi Matsuura, Kenshiro Shikano, Takaya Saito, Eiko Iwakoshi-Ukena, Megumi Furumitsu, Yuta Ochi, Manami Sato, George E Bentley, Lance J Kriegsfeld, Kazuyoshi Ukena
We have recently identified a novel cDNA encoding a small secretory protein termed neurosecretory protein GL (NPGL) from the avian hypothalamus. In chicks, NPGL increases body weight gain without affecting food intake. A database search reveals that NPGL is conserved throughout vertebrates. However, the central distribution and functional role of NPGL remains to be elucidated in mammals. In this study, we identified the precursor cDNA encoding NPGL from the mouse hypothalamus. Quantitative RT-PCR and morphological analyses revealed that NPGL precursor mRNA is robustly expressed in the mediobasal hypothalamus with NPGL neurons specifically localized to the lateroposterior part of the arcuate nucleus in the hypothalamus...
March 17, 2017: Endocrinology
https://www.readbyqxmd.com/read/28323951/assessing-energy-requirements-in-women-with-polycystic-ovary-syndrome-a-comparison-against-doubly-labeled-water
#14
Nicholas T Broskey, Monica C Klempel, L Anne Gilmore, Elizabeth F Sutton, Abby D Altazan, Jeffrey H Burton, Eric Ravussin, Leanne M Redman
Context: Weight loss is prescribed to offset the deleterious consequences of polycystic ovary syndrome (PCOS), but a successful intervention requires an accurate assessment of energy requirements. Objective: Describe energy requirements in women with PCOS and evaluate common prediction equations compared to doubly-labeled water (DLW). Design: Cross-sectional study. Setting: Academic research center. Participants: Twenty-eight weight-stable women with PCOS completed a 14-day DLW study along with measures of body composition, resting metabolic rate, and physical activity assessment by accelerometry...
March 8, 2017: Journal of Clinical Endocrinology and Metabolism
https://www.readbyqxmd.com/read/28322645/the-effects-of-a-health-partner-program-on-reducing-cardiovascular-risk-factors
#15
Tingting Liu
Previous lifestyle intervention research targeting prediabetes has primarily focused on weight loss, blood glucose, and lipid abnormalities. Far less is known whether lifestyle interventions lower other biological and physical risk factors for cardiovascular disease in overweight/obese adults with or without prediabetes. The aim of the study was to determine if a health partner program reduced biological and physical cardiovascular risk factors in overweight/obese adults with and without prediabetes at 1 year...
December 1, 2016: Western Journal of Nursing Research
https://www.readbyqxmd.com/read/28322513/site-of-metabolism-prediction-based-on-ab-initio-derived-atom-representations
#16
Arndt R Finkelmann, Andreas H Göller, Gisbert Schneider
Machine learning models for site of metabolism (SoM) prediction offer the ability to identify metabolic soft spots in low molecular weight drug molecules at low computational cost and enable data-based reactivity prediction. SoM prediction is an atom classification problem. Successful construction of machine learning models requires atom representations that capture the reactivity-determining features of a potential reaction site. We have developed a descriptor scheme that characterizes an atom's steric and electronic environment and its relative location in the molecular structure...
March 21, 2017: ChemMedChem
https://www.readbyqxmd.com/read/28321904/rotating-single-shot-acquisition-rosa-with-composite-reconstruction-for-fast-high-resolution-diffusion-imaging
#17
Qiuting Wen, Chandana Kodiweera, Brian M Dale, Giri Shivraman, Yu-Chien Wu
PURPOSE: To accelerate high-resolution diffusion imaging, rotating single-shot acquisition (RoSA) with composite reconstruction is proposed. Acceleration was achieved by acquiring only one rotating single-shot blade per diffusion direction, and high-resolution diffusion-weighted (DW) images were reconstructed by using similarities of neighboring DW images. A parallel imaging technique was implemented in RoSA to further improve the image quality and acquisition speed. RoSA performance was evaluated by simulation and human experiments...
March 20, 2017: Magnetic Resonance in Medicine: Official Journal of the Society of Magnetic Resonance in Medicine
https://www.readbyqxmd.com/read/28321031/role-of-ketotifen-on-metabolic-profiles-inflammation-and-oxidative-stress-in-diabetic-rats
#18
Zimiao Chen, Hongwei Sun, Jian Wang, Liansong Ni, Xuejiang Gu, Shengjie Ge, Qiong Chen, Liangshan Mu, Xingbo Cheng
We aim to explore effects of Ketotifen on metabolic profiles, inflammation and oxidative stress. Sprague Dawley (SD) male rats were randomly divided into normal control group (NC) and experimental groups, and experimental group rats were fed with high-sugar and fat diet for 6 weeks. Then, experimental group rats were divided into diabetes group (DM) and ketotifen treatment group (KT). KT group was given ketotifen via Intragastric for 8 weeks with the dosage of 0.09 mg/kg•d. Fasting plasma glucose (FPG) was measured using glucose oxidase-phenol amino phenazone method...
March 17, 2017: Endocrine Journal
https://www.readbyqxmd.com/read/28321030/pancreas-volume-reduction-and-metabolic-effects-in-japanese-patients-with-severe-obesity-following-laparoscopic-sleeve-gastrectomy
#19
Akira Umemura, Akira Sasaki, Hiroyuki Nitta, Shigeaki Baba, Taro Ando, Takashi Kajiwara, Yasushi Ishigaki
This study aimed to assess the relationship between the metabolic effect after laparoscopic sleeve gastrectomy (LSG) in morbidly obese Japanese patients, with or without type 2 diabetes mellitus (T2DM), and improved pancreatic steatosis (PS). The study enrolled 27 morbidly obese Japanese patients who were undergoing LSG. Their clinical and metabolic effects were evaluated at baseline and six months after LSG. Pancreas volume (PV), pancreatic attenuation (PA), and splenic attenuation (SA) were measured using a 64-row computed tomography (CT)...
March 17, 2017: Endocrine Journal
https://www.readbyqxmd.com/read/28320522/-image-guided-bone-consolidation-in-oncology-cementoplasty-and-percutaneous-screw-fixation
#20
REVIEW
Xavier Buy, Roberto Luigi Cazzato, Vittorio Catena, Guilhem Roubaud, Michele Kind, Jean Palussiere
Bone metastases are a common finding in oncology. They often induce pain but also fractures which impair quality of life, especially when involving weight-bearing bones. Percutaneous image-guided consolidation techniques play a major role for the management of bone metastases. Cementoplasty aims to stabilize bone and control pain by injecting acrylic cement into a weakened bone. This minimally invasive technique has proven its efficacy for bones submitted to compression forces: vertebra, acetabular roof, and condyles...
March 17, 2017: Bulletin du Cancer
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