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https://www.readbyqxmd.com/read/28186296/entamoeba-histolytica-encoded-homolog-of-macrophage-migration-inhibitory-factor-contributes-to-mucosal-inflammation-during-amebic-colitis
#1
Renay Ngobeni, Mayuresh M Abhyankar, Nona M Jiang, Laura A Farr, Amidou Samie, Rashidul Haque, Shannon N Moonah
No abstract text is available yet for this article.
February 10, 2017: Journal of Infectious Diseases
https://www.readbyqxmd.com/read/28182670/killing-of-diverse-eye-pathogens-acanthamoeba-spp-fusarium-solani-and-chlamydia-trachomatis-with-alcohols
#2
Yousuf Aqeel, Raquel Rodriguez, Aparajita Chatterjee, Robin R Ingalls, John Samuelson
BACKGROUND: Blindness is caused by eye pathogens that include a free-living protist (Acanthamoeba castellanii, A. byersi, and/or other Acanthamoeba spp.), a fungus (Fusarium solani), and a bacterium (Chlamydia trachomatis). Hand-eye contact is likely a contributor to the spread of these pathogens, and so hand washing with soap and water or alcohol-based hand sanitizers (when water is not available) might reduce their transmission. Recently we showed that ethanol and isopropanol in concentrations present in hand sanitizers kill walled cysts of Giardia and Entamoeba, causes of diarrhea and dysentery, respectively...
February 9, 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28182579/synthesis-and-biological-evaluation-of-newer-1-3-4-oxadiazoles-incorporated-with-benzothiazepine-and-benzodiazepine-moieties
#3
Patel Navin, Patel Sarvil, Purohit Amit, Patel Divyesh, Rajani Dhansukh, Rosa Moo-Puc, Gildardo Rivera
A series of thiazepines and diazepines having 1,3,4-oxadiazole moiety were synthesized, and they were analyzed for their in vitro antimicrobial activity against several bacteria (Staphylococcus aureus, Staphylococcus pyogenes, Escherichia coli, and Pseudomonas aeruginosa) and fungi (Candida albicans, Aspergillus niger, and Aspergillus Clavatus) and protozoa (Entamoeba histolytica, Giardia lamblia, Trypanosoma cruzi and Leishmania mexicana). Few of the selected compounds were tested for their antitubercular activity...
February 9, 2017: Zeitschrift Für Naturforschung. C, A Journal of Biosciences
https://www.readbyqxmd.com/read/28180910/-ambiguous-hepatic-mass-and-fever-after-stay-in-turkey
#4
J Mueller, S Dagenbach, B Riecken, C Klinger
We report the case of a 76-year old female patient with a hepatic mass after staying in eastern Turkey. There were no indices for malignancy or an infection with Echinococcus or Entamoeba histolytica. Finally we diagnosed a Fascioliasis (liver fluke) and cured the patient successfully.
February 8, 2017: Der Internist
https://www.readbyqxmd.com/read/28167597/epidemiology-of-soil-transmitted-helminth-and-intestinal-protozoan-infections-in-preschool-aged-children-in-the-amhara-region-of-ethiopia
#5
Kristen Aiemjoy, Sintayehu Gebresillasie, Nicole E Stoller, Ayalew Shiferaw, Zerihun Tadesse, Melsew Chanyalew, Solomon Aragie, Kelly Callahan, Jeremy D Keenan
Intestinal parasites are important contributors to global morbidity and mortality and are the second most common cause of outpatient morbidity in Ethiopia. This cross-sectional survey describes the prevalence of soil-transmitted helminths and intestinal protozoa in preschool children 0-5 years of age in seven communities in the Amhara region of Ethiopia, and investigates associations between infection, household water and sanitation characteristics, and child growth. Stool samples were collected from children 0-5 years of age, 1 g of sample was preserved in sodium acetate-acetic acid-formalin, and examined for intestinal helminth eggs and protozoa cysts ether-concentration method...
February 6, 2017: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/28167596/epidemiology-and-prevalence-of-blastocystis-spp-in-north-cyprus
#6
Ayse Seyer, Djursun Karasartova, Emrah Ruh, Ayse Semra Güreser, Ebru Turgal, Turgut Imir, Aysegul Taylan Ozkan
This study was conducted to investigate the prevalence of Blastocystis spp. and its subtypes (STs) in North Cyprus; and to evaluate the presence of this parasite and its STs with respect to demographic, socioeconomic, and epidemiological factors, as well as gastrointestinal symptoms. Stool samples were collected from 230 volunteers. Each participant also filled out a questionnaire. The samples were examined microscopically by native-Lugol and trichrome methods and further tested by polymerase chain reaction (PCR) and sequencing...
February 6, 2017: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/28151567/first-molecular-epidemiology-of-entamoeba-histolytica-e-dispar-and-e-moshkovskii-infections-in-yemen-different-species-specific-associated-risk-factors
#7
Mona A Al-Areeqi, Hany Sady, Hesham M Al-Mekhlafi, Tengku Shahrul Anuar, Abdulelah H Al-Adhroey, Wahib M Atroosh, Salwa Dawaki, Fatin Nur Elyana, Nabil A Nasr, Init Ithoi, Yee-Ling Lau, Johari Surin
OBJECTIVES: To investigate the molecular epidemiology of Entamoeba histolytica, E. dispar and E. moshkovskii infections among rural communities in Yemen. METHODS: In a community-based study, faecal samples were collected from 605 participants and examined by wet mount, formalin-ether sedimentation, trichrome staining and nested multiplex PCR techniques. Demographic, socioeconomic and environmental information was collected by using a pre-tested questionnaire. RESULTS: Overall, 324 (53...
February 2, 2017: Tropical Medicine & International Health: TM & IH
https://www.readbyqxmd.com/read/28134428/prevalence-and-seasonal-variation-of-human-intestinal-parasites-in-patients-attending-hospital-with-abdominal-symptoms-in-northern-jordan
#8
A S Jaran
This study was carried out to determine the prevalence of intestinal parasites and their seasonal variation in northern Jordan. A total of 21 906 stool samples were collected over a period of 4 years (2009- 2013) from 5 government hospitals in 3 cities. Samples were processed and examined microscopically and by concentration methods. Parasitic infection was found in 9611 samples (44%). Giardia lamblia was the most prevalent parasite (41%) followed by Entamoeba histolytica (31%) and Ent. coli (13%); the least prevalent parasites were Ascaris lumbricoides (1%), Hymenolepis nana, Taenia sp...
January 23, 2017: Eastern Mediterranean Health Journal, la Revue de Santé de la Méditerranée Orientale
https://www.readbyqxmd.com/read/28131659/sine-polymorphism-reveals-distinct-strains-of-entamoeba-histolytica-from-north-india
#9
Shraddha Sharma, Neha Banyal, Mukul Singh, A K Mandal, Sudha Bhattacharya, Jaishree Paul
Entamoeba histolytica is an intestinal parasite causing significant morbidity and mortality in the developing world. More tools are needed to understand the epidemiology and molecular pathogenesis of amebiasis. Virulence pattern of E. histolytica could be linked with the genotype of a strain. Several loci showing insertion polymorphism of retrotransposable short interspersed nuclear elements EhSINE1 and EhSINE2 have been reported among laboratory strains of E. histolytica. The present study was undertaken to validate this observation in clinical isolates from north India...
January 26, 2017: Experimental Parasitology
https://www.readbyqxmd.com/read/28130101/intestinal-parasitic-infections-in-iranian-preschool-and-school-children-a-systematic-review-and-meta-analysis
#10
REVIEW
Ahmad Daryani, Saeed Hosseini-Teshnizi, Seyed-Abdollah Hosseini, Ehsan Ahmadpour, Shahabeddin Sarvi, Afsaneh Amouei, Azadeh Mizani, Sara Gholami, Mehdi Sharif
Parasitic infections are a serious public health problem because they cause anemia, growth retardation, aggression, weight loss, and other physical and mental health problems, especially in children. Numerous studies have been performed on intestinal parasitic infections in Iranian preschool and school children. However, no study has gathered and analyzed this information systematically. The aim of this study was to provide summary estimates for the available data on intestinal parasitic infections in Iranian children...
January 24, 2017: Acta Tropica
https://www.readbyqxmd.com/read/28127364/prevalence-of-strongyloides-stercoralis-and-other-intestinal-parasites-among-institutionalized-mentally-disabled-individuals-in-rasht-northern-iran
#11
Amin Saeidinia, Ilnaz Tavakoli, Mohammad Reza Naghipour, Behnaz Rahmati, Hossein Ghavami Lahiji, Omid Salkhori, Keyhan Ashrafi
BACKGROUND: We aimed to determine the status of strongyloidiasis in mentally disabled population in the institutional places in Rasht City, the capital of Guilan Province, northern Iran. METHODS: This cross-sectional study was conducted in 8 institutions for mentally retarded population in Rasht in 2013. Before collecting the samples, a questionnaire was filled out for each participant by an expert person. A single stool sample was obtained from each of the 173 subjects and examined using direct wet mount, formalin-ether concentration technique and agar plate culture method...
October 2016: Iranian Journal of Parasitology
https://www.readbyqxmd.com/read/28126902/the-monomeric-gtpase-rab35-regulates-phagocytic-cup-formation-and-phagosomal-maturation-in-entamoeba-histolytica
#12
Kuldeep Verma, Sunando Datta
One of the hallmarks of amoebic colitis is the detection of Entamoeba histolytica (Eh) trophozoites with ingested erythrocytes. Therefore, erythrophagocytosis is traditionally considered as one of the most important criteria to identify the pathogenic behavior of the amoebic trophozoites. Phagocytosis is an essential process for the proliferation and virulence of this parasite. Phagocytic cargo, upon internalization, follows defined trafficking route to amoebic lysosomal degradation machinery. Here, we demonstrated the role of EhRab35 in the early and late phase of erythrophagocytosis by the amoeba...
January 26, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28100304/iron-responsive-like-elements-in-the-parasite-entamoeba-histolytica
#13
Liliana Soto-Castro, Laura Yuliana Plata-Guzman, Elisa Elvira Figueroa-Angulo, Jaeson Calla-Choque, Magda Reyes-Lopez, Mireya DE LA Garza, Nidia Leon-Sicairos, Jose Antonio Garzon-Tiznado, Rossana Arroyo, Claudia Leon-Sicairos
In Entamoeba histolytica, iron modulates virulence and gene expression via unknown regulatory mechanisms. The existence of a posttranscriptional iron regulatory system parallel to the IRE/IRP (Iron-Responsive Element/Iron Regulatory Protein) system in the protozoan Trichomonas vaginalis has recently been reported. Due to their evolutionary closeness and the importance of iron for growth and virulence in these protozoa, we hypothesized the existence of an IRE/IRP-like mechanism in E. histolytica. To determine the presence of IRE-like elements in some mRNAs from this parasite, we performed in silico analyses of the 5'- and 3'-UTRs of mRNAs encoding virulence factors and cytoskeleton, ribosomal, and metabolism proteins...
January 18, 2017: Microbiology
https://www.readbyqxmd.com/read/28093543/intestinal-parasite-infections-and-accuracy-of-direct-thin-and-thick-smear-formol-ether-sedimentation-centrifugal-flotation-and-mini-flotac-techniques-among-patients-with-gastrointestinal-tract-disorders-from-the-greater-cairo-region-egypt
#14
Atef H Hussein, Samia M Rashed, Ibrahim A El-Hayawan, Nagwa S Ali, Eman A Abou Ouf, Amira T Ali
The aim of the present study was to assess the frequency of intestinal parasitic infection among patients with gastrointestinal tract disorders from the Greater Cairo region, Egypt. In addition, a comparison was made of the accuracy of direct thin and thick smear, formol-ether sedimentation (FEC), centrifugal flotation (CF), and mini-FLOTAC techniques in the diagnosis of infection. Out of 100 patients, the overall prevalence of parasitic infection was 51%. Only 6% had dual infection. Giardia lamblia was the most common parasite (26%), followed by Hymenolepis nana (20%), Entamoeba coli (8%), and Enterobius vermicularis (3%)...
January 16, 2017: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/28089548/nanoformulation-of-synergistic-tlr-ligands-to-enhance-vaccination-against-entamoeba-histolytica
#15
Mayuresh M Abhyankar, Zannatun Noor, Mark A Tomai, James Elvecrog, Christopher B Fox, William A Petri
Diarrheal infectious diseases represent a major cause of global morbidity and mortality. There is an urgent need for vaccines against diarrheal pathogens, especially parasites. Modern subunit vaccines rely on combining a highly purified antigen with an adjuvant to increase their efficacy. In the present study, we evaluated the ability of a nanoliposome adjuvant system to trigger a strong mucosal immune response to the Entamoeba histolytica Gal/GalNAc lectin LecA antigen. CBA/J mice were immunized with alum, emulsion or liposome based formulations containing synthetic TLR agonists...
February 7, 2017: Vaccine
https://www.readbyqxmd.com/read/28087959/a-study-on-hla-dr-dq-typing-in-adult-malay-patients-with-acute-amoebic-liver-abscess
#16
Z Nazli, N M S Azira, M Zeehaida, Z Nik Zairi, Y Nurul Khaiza, Y Maya Mazuwin
INTRODUCTION: Amoebiasis is a parasitic disease caused by Entamoeba histolytica that may lead to death in developing countries. Few important risk factors have been identified in the development of amoebic liver abscess (ALA). There are limited reports that suggest an association between antigens of the major histocompatibility complex (MHC) particularly class II antigens and ALA development. This present work aimed at studying the possible association of HLA antigens with ALA and disease severity...
December 2016: Medical Journal of Malaysia
https://www.readbyqxmd.com/read/28070012/prevalence-and-risk-factors-for-intestinal-parasitic-infection-in-schoolchildren-in-battambang-cambodia
#17
Chien-Wei Liao, Kuan-Chih Chiu, I-Chen Chiang, Po-Ching Cheng, Ting-Wu Chuang, Juo-Han Kuo, Yun-Hung Tu, Chia-Kwung Fan
Most intestinal parasitic infections (IPIs), commonly endemic in tropical resource-poor developing countries, are neglected tropical diseases. Parasitic infections and malnutrition are most commonly found in children. We determined the prevalence of IPIs and the risk factors in Battambang Province, northwestern Cambodia, from August to September 2015. This study collected 308 valid questionnaires and specimens from Dontri (173, 56.2%) and Kon Kaêk (135, 43.8%) primary schools. All stool samples were examined using Chang's Feces Examination Apparatus through the merthiolate-iodine-formaldehyde technique...
January 9, 2017: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/28069814/muc2-mucin-and-butyrate-contribute-to-the-synthesis-of-the-antimicrobial-peptide-cathelicidin-in-response-to-e-histolytica-and-dss-induced-colitis
#18
Eduardo R Cobo, Vanessa Kissoon-Singh, France Moreau, Ravi Holani, Kris Chadee
Embedded in the colonic mucus are cathelicidins, small cationic peptides secreted by colonic epithelial cells. Humans and mice have one cathelicidin-related antimicrobial peptide (CRAMP), LL-37/hCAP-18 and Cramp respectively, with related structure and functions. Altered production of MUC2 mucin and antimicrobial peptides is characteristic of intestinal amebiasis. The interactions between MUC2 mucin and cathelicidins in conferring innate immunity against Entamoeba histolytica (Eh) are not well characterized...
January 9, 2017: Infection and Immunity
https://www.readbyqxmd.com/read/28061872/amoebic-liver-abscess-in-northern-sri-lanka-first-report-of-immunological-and-molecular-confirmation-of-aetiology
#19
Selvam Kannathasan, Arumugam Murugananthan, Thirunavukarasu Kumanan, Devika Iddawala, Nilanthi Renuka de Silva, Nadarajah Rajeshkannan, Rashidul Haque
BACKGROUND: Since 1985, amoebic liver abscess (ALA) has been a public health problem in northern Sri Lanka. Clinicians arrive at a diagnosis based on clinical and ultrasonographic findings, which cannot differentiate pyogenic liver abscess (PLA) from ALA. As the treatment and outcome of the ALA and PLA differs, determining the etiological agent is crucial. METHODS: All clinically diagnosed ALA patients admitted to the Teaching Hospital (TH) in Jaffna during the study period were included and the clinical features, haematological parameters, and ultrasound scanning findings were obtained...
January 7, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/28030342/molecular-diagnosis-of-entamoeba-spp-versus-microscopy-in-the-great-cairo
#20
Mohamed H Roshdy, Nour M Abd El-Kader, Marwa Ali-Tammam, Isabel Fuentes, Magdy M Mohamed, Nabila A El-Sheikh, Jose Miguel Rubio
Amoebiasis is a human disease produced by Entamoeba histolytica which causes widespread mortality and morbidity worldwide through diarrheal disease and abscess establishment in parenchymal tissues such as liver, lung, and brain. The true prevalence of infection is unknown for most areas of the world due to the difficulty to characterise Entamoeba histolytica versus other non-pathogenic amoebas with identical morphology, as Entamoeba dispar, and Entamoeba moshkovskii. To overcome microscopy misidentification issues, we tested a nested multiplex polymerase chain reaction (PCR) and a real-time PCR on 194 stool samples collected from incoming dysentery patients in Cairo hospitals diagnosed with E...
March 1, 2017: Acta Parasitologica
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