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https://www.readbyqxmd.com/read/28416464/microbiological-quality-of-water-in-a-city-with-persistent-and-recurrent-waterborne-diseases-under-tropical-sub-rural-conditions-the-case-of-kikwit-city-democratic-republic-of-the-congo
#1
Alexis B Nienie, Periyasamy Sivalingam, Amandine Laffite, Patience Ngelinkoto, Jean-Paul Otamonga, Alphonse Matand, Crispin K Mulaji, Emmanuel M Biey, Pius T Mpiana, John Poté
The availability of safe drinking water in sub-Saharan countries remains a major challenge because poor sanitation has been the cause of various outbreaks of waterborne disease due to the poor microbiological quality of water used for domestic purposes. The faecal indicator bacteria (FIB) used in the present study included Escherichia coli (E. coli) and Enterococcus (ENT). FIB and aerobic mesophilic bacteria (AMB) were quantified during July 2015 (dry season) and November 2015 (rainy season) in order to assess the quality of drinking water from wells (n=3; P1-P3), and two rivers, the River Lukemi (RLK, n=3) and River Luini (RLN, n=2) in the city of Kikwit, which is located in the province of Kwilu in the Democratic Republic of the Congo...
March 30, 2017: International Journal of Hygiene and Environmental Health
https://www.readbyqxmd.com/read/28390155/employing-binol-phosphoroselenoyl-chloride-for-selective-inositol-phosphorylation-and-chemical-synthesis-of-glycosyl-inositol-phospholipid-from-entamoeba-histolytica
#2
Toshihiko Aiba, Sae Suehara, Siew-Ling Choy, Yuuki Maekawa, Hannelore Lotter, Toshiaki Murai, Shinsuke Inuki, Koichi Fukase, Yukari Fujimoto
Here we describe the chemical synthesis of glycosyl inositol phospholipids from Entamoeba histolytica. The key feature of this synthesis is a regioselective phosphorylation reaction that occurs via desymmetrization of a myo-inositol derivative with phosphoroselenoyl chloride. We also developed a new protecting group strategy that utilizes allyl and alloc groups to synthesize complex glycolipids bearing unsaturated lipids. These developments provided an efficient synthetic route for various complex inositol phospholipids and their analogues...
April 8, 2017: Chemistry: a European Journal
https://www.readbyqxmd.com/read/28380216/detection-and-differentiation-of-entamoeba-histolytica-and-entamoeba-dispar-in-clinical-samples-through-pcr-denaturing-gradient-gel-electrophoresis
#3
P López-López, M C Martínez-López, X M Boldo-León, Y Hernández-Díaz, T B González-Castro, C A Tovilla-Zárate, J P Luna-Arias
Amebiasis is one of the twenty major causes of disease in Mexico; however, the diagnosis is difficult due to limitations of conventional microscopy-based techniques. In this study, we analyzed stool samples using polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) to differentiate between Entamoeba histolytica (pathogenic) and E. dispar (non-pathogenic). The target for the PCR amplification was a small region (228 bp) of the adh112 gene selected to increase the sensitivity of the test...
April 3, 2017: Brazilian Journal of Medical and Biological Research, Revista Brasileira de Pesquisas Médicas e Biológicas
https://www.readbyqxmd.com/read/28378758/corrigendum-n-acetyl-ornithine-deacetylase-is-a-moonlighting-protein-and-is-involved-in-the-adaptation-of-entamoeba-histolytica-to-nitrosative-stress
#4
Preeti Shahi, Meirav Trebicz-Geffen, Shruti Nagaraja, Rivka Hertz, Sharon Baumel-Alterzon, Karen Methling, Michael Lalk, Mohit Mazumder, Gourinath Samudrala, Serge Ankri
No abstract text is available yet for this article.
April 5, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28375117/clinical-study-on-the-efficacy-of-amoebex-coded-herbal-drug-compared-with-metronidazole-for-the-treatment-of-amoebic-dysentery
#5
Syed MuhammadAli Shah, Khan Usmanghani, Naveed Akhtar, Muhammad Akram, Hafiz Muhammad Asif, Muhammad Mohtasheemul Hasan
Amoebiasisis an infectious disease, which originated with the single-celled parasitic protozoan Entamoeba histolytica. The parasitic amoeba infects the liver and intestine and may cause mild diarrhea and serious dysentery with bloody and mucoid stool. A study was conducted to evaluate the efficacy of Amoebex (400mg), a herbal formulation for the treatment of amoebiasis infections as compared to that of Metronidazole (400mg). The therapeutic evaluations of these medicines were carried out on 184 clinically diagnosed cases of the amoebiasis infection...
November 2016: Pakistan Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/28372729/development-of-a-thermostabilised-triplex-lamp-assay-with-dry-reagent-four-target-lateral-flow-dipstick-for-detection-of-entamoeba-histolytica-and-non-pathogenic-entamoeba-spp
#6
Phiaw Chong Foo, Yean Yean Chan, Maizan Mohamed, Weng Kin Wong, A B Nurul Najian, Boon Huat Lim
This study highlighted the development of a four target nitrocellulose-based nucleic acid lateral flow immunoassay biosensor in a dry-reagent strip format for interpretation of double-labelled double-stranded amplicons from thermostabilised triplex loop-mediated isothermal amplification assay. The DNA biosensor contained two test lines which captured biotin and texas red labelled amplicons; a LAMP internal amplification control line that captured digoxigenin labelled amplicon; and a chromatography control line that validated the functionality of the conjugated gold nanoparticles and membrane...
May 8, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28366172/the-effects-of-phosphanegold-i-thiolates-on-the-biological-properties-of-acanthamoeba-castellanii-belonging-to-the-t4-genotype
#7
Ruqaiyyah Siddiqui, Farhat Abjani, Chien Ing Yeo, Edward R T Tiekink, Naveed Ahmed Khan
BACKGROUND: Gold compounds have shown promise in the treatment of non-communicable diseases such as rheumatoid arthritis and cancer, and are considered of value as anti-microbial agents against Gram-negative and Gram-positive bacteria, and have anti-parasitic properties against Schistosoma mansoni, Trypanosoma brucei, Plasmodium falciparum, Leishmania infantinum, Giardia lamblia, and Entamoeba histolytica. They are known to affect enzymatic activities that are required for the cellular respiration processes...
April 3, 2017: Journal of Negative Results in Biomedicine
https://www.readbyqxmd.com/read/28359328/stem-loop-mediated-isothermal-amplification-test-comparative-analysis-with-classical-lamp-and-pcr-in-detection-of-entamoeba-histolytica-in-kenya
#8
COMPARATIVE STUDY
Fridah Mwendwa, Cecilia K Mbae, Johnson Kinyua, Erastus Mulinge, Gitonga Nkanata Mburugu, Zablon K Njiru
BACKGROUND: Entamoeba histolytica, the causative agent for amoebiasis is a considerable burden to population in the developing countries where it accounts for over 50 million infections. The tools for detection of amoebiasis are inadequate and diagnosis relies on microscopy which means a significant percent of cases remain undiagnosed. Moreover, tests formats that can be rapidly applied in rural endemic areas are not available. METHODS: In this study, a loop-mediated isothermal test (LAMP) based on 18S small subunit ribosomal RNA gene was designed with extra reaction accelerating primers (stem primers) and compared with the published LAMP and PCR tests in detection of E...
March 31, 2017: BMC Research Notes
https://www.readbyqxmd.com/read/28348756/amoeboma-in-a-saudi-resident-a-case-report
#9
Sanaa Al Rehily, Reham Kaki, Fahad Al Ghamdi, Dalia El-Hossary
INTRODUCTION: Amoebiasis is the third most frequent cause of mortality after malaria and schistosomiasis. In developed countries, amebiasis is also seen in migrants who have travelled to endemic areas. The factors responsible for its progression from intestinal amebiasis to an amebic liver abscess are not fully understood. CASE PRESENTATION: A 54-year-old man presented with abdominal pain, fever and diarrhoea. Laparotomy confirmed an inflammatory mass involving the right colon, and he underwent a right hemicolectomy...
June 2016: JMM Case Reports
https://www.readbyqxmd.com/read/28335696/development-and-initial-evaluation-of-a-lateral-flow-dipstick-test-for-antigen-detection-of-entamoeba-histolytica-in-stool-sample
#10
Syazwan Saidin, Muhammad Hafiznur Yunus, Nurulhasanah Othman, Yvonne Ai-Lian Lim, Zeehaida Mohamed, Nik Zairi Zakaria, Rahmah Noordin
Entamoeba histolytica infection remains a public health concern in developing countries. Early diagnosis of amoebiasis can avoid disease complications, thus this study was aimed at developing a test that can rapidly detect the parasite antigens in stool samples. Rabbits were individually immunized with recombinant pyruvate phosphate dikinase (rPPDK) and E. histolytica excretory-secretory antigens to produce polyclonal antibodies. A rapid dipstick test was produced using anti-rPPDK PAb lined on the dipstick as capture reagent and anti-EhESA PAb conjugated to colloidal gold as the detector reagent...
March 24, 2017: Pathogens and Global Health
https://www.readbyqxmd.com/read/28317870/structural-and-functional-studies-of-a-noncanonical-dicer-from-entamoeba-histolytica
#11
Xiang Yu, Xuhang Li, Lina Zheng, Jinbiao Ma, Jianhua Gan
RNaseIII proteins are dsRNA-specific endonucleases involved in many important biological processes, such as small RNA processing and maturation in eukaryotes. Various small RNAs have been identified in a protozoan parasite Entamoeba histolytica. EhRNaseIII is the only RNaseIII endonuclease domain (RIIID)-containing protein in E. histolytica. Here, we present three crystal structures that reveal several unique structural features of EhRNaseIII, especially the interactions between the two helixes (α1 and α7) flanking the RIIID core domain...
March 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28302528/association-between-enteric-protozoan-parasites-and-gastrointestinal-illness-among-hiv-and-tuberculosis-infected-individuals-in-the-chowke-district-southern-mozambique
#12
María José Irisarri-Gutiérrez, Marta Hernández-de Mingo, Aida de Lucio, Horacio Gil, Lucía Morales, Raimundo Seguí, Edy Nacarapa, Carla Muñoz-Antolí, Fernando Jorge Bornay-Llinares, José Guillermo Esteban, David Carmena
Human immune deficiency virus (HIV) and tuberculosis (TB) infections remain major public health issues globally, particularly in sub-Saharan Africa. Impairment of both cell-mediated and humoral immunity by HIV and/or TB infections may limit the host's defences against other pathogens, including the diarrheagenic protozoan Cryptosporidium spp., Giardia intestinalis, and Entamoeba histolytica. During September-December 2015 a cross-sectional study was conducted to assess the prevalence and molecular diversity of these enteric parasites among HIV- and/or TB-infected patients at a medical reference centre in Chowke district, southern Mozambique...
March 14, 2017: Acta Tropica
https://www.readbyqxmd.com/read/28293543/the-tudor-staphylococcal-nuclease-protein-of-entamoeba-histolytica-participates-in-transcription-regulation-and-stress-response
#13
Javier Cázares-Apátiga, Christian Medina-Gómez, Bibiana Chávez-Munguía, Mercedes Calixto-Gálvez, Esther Orozco, Carlos Vázquez-Calzada, Aarón Martínez-Higuera, Mario A Rodríguez
Entamoeba histolytica is the protozoa parasite responsible of human amoebiasis, disease that causes from 40,000 to 100,000 deaths annually worldwide. However, few are known about the expression regulation of molecules involved in its pathogenicity. Transcription of some virulence-related genes is positively controlled by the cis-regulatory element named URE1. Previously we identified the transcription factor that binds to URE1, which displayed a nuclear and cytoplasmic localization. This protein belongs to the Tudor Staphyococcal nuclease (TSN) family, which in other systems participates in virtually all pathways of gene expression, suggesting that this amoebic transcription factor (EhTSN; former EhURE1BP) could also play multiple functions in E...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28293411/a-strange-manifestation-of-malaria-in-a-native-nigerian-boy
#14
Paola Magro, Ilaria Izzo, Barbara Saccani, Salvatore Casari, Silvio Caligaris, Lina Rachele Tomasoni, Alberto Matteelli, Annamaria Lombardi, Antonella Meini, Francesco Castelli
The protective role of Sickle Cell Trait (SCT) in malaria endemic areas has been proved, and prevalence of HbS gene in malaria endemic areas is high. Splenic infarction is a well-known complication of SCT, while the association with malaria is considered rare. A Nigerian boy was admitted to our ward after returning from his country of origin, for P. falciparum malaria. He underwent abdominal ultrasound for upper right abdominal pain, showing cholecystitis and multiple splenic lesions suggestive of abscesses...
2017: Mediterranean Journal of Hematology and Infectious Diseases
https://www.readbyqxmd.com/read/28287148/behavior-of-dna-lacking-mitochondria-in-entamoeba-histolytica-revealed-by-organelle-transplant
#15
Makoto Kazama, Sanae Ogiwara, Takashi Makiuchi, Kazuhiro Yoshida, Kumiko Nakada-Tsukui, Tomoyoshi Nozaki, Hiroshi Tachibana
The anaerobic protozoan parasite Entamoeba histolytica has mitosomes that are mitochondria lacking some canonical functions and organelle DNA. Mitosomes play an important role in the life cycle of the parasite. The distribution of proteins in mitosomes is not uniform, and how mitosomes are maintained and retained is unknown. To answer these questions, we developed a transplant method for mitosomes with hemagglutinin-tagged protein into recipient cells containing mitosomes with Myc-tagged protein. Immunofluorescence staining showed that the two protein tags colocalized in single mitosomes in some recipient cells...
March 13, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28273080/geospatial-distribution-of-intestinal-parasitic-infections-in-rio-de-janeiro-brazil-and-its-association-with-social-determinants
#16
Clarissa Perez Faria, Graziela Maria Zanini, Gisele Silva Dias, Sidnei da Silva, Marcelo Bessa de Freitas, Ricardo Almendra, Paula Santana, Maria do Céu Sousa
BACKGROUND: Intestinal parasitic infections remain among the most common infectious diseases worldwide. This study aimed to estimate their prevalence and provide a detailed analysis of geographical distribution of intestinal parasites in the metropolitan region of Rio de Janeiro, considering demographic, socio-economic, and epidemiological contextual factors. METHODS/PRINCIPAL FINDINGS: The cross-section survey was conducted among individuals attending the Evandro Chagas National Institute of Infectious Diseases (FIOCRUZ, RJ) during the period from April 2012 to February 2015...
March 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28270802/molecular-epidemiology-of-giardia-blastocystis-and-cryptosporidium-among-indigenous-children-from-the-colombian-amazon-basin
#17
Angie Sánchez, Marina Munoz, Natalia Gómez, Juan Tabares, Laura Segura, Ángela Salazar, Cristian Restrepo, Miguel Ruíz, Patricia Reyes, Yuchen Qian, Lihua Xiao, Myriam C López, Juan D Ramírez
The incidence and prevalence of intestinal parasites in children is most likely due to lack of natural or acquired resistance and differences in behavior and habits closely related to environmental and socioeconomic determinants. The most important protozoa that parasitize humans are Giardia, Entamoeba, Blastocystis, and Cryptosporidium. These parasites present wide intraspecific genetic diversity and subsequently classified into assemblages and subtypes. The Amazon basin is the largest in the world and is the fifth freshwater reserve on the planet...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28246365/role-of-eosinophils-and-tumor-necrosis-factor-alpha-in-interleukin-25-mediated-protection-from-amebic-colitis
#18
Zannatun Noor, Koji Watanabe, Mayuresh M Abhyankar, Stacey L Burgess, Erica L Buonomo, Carrie A Cowardin, William A Petri
The parasite Entamoeba histolytica is a cause of diarrhea in infants in low-income countries. Previously, it was shown that tumor necrosis factor alpha (TNF-α) production was associated with increased risk of E. histolytica diarrhea in children. Interleukin-25 (IL-25) is a cytokine that is produced by intestinal epithelial cells that has a role in maintenance of gut barrier function and inhibition of TNF-α production. IL-25 expression was decreased in humans and in the mouse model of amebic colitis. Repletion of IL-25 blocked E...
February 28, 2017: MBio
https://www.readbyqxmd.com/read/28229045/occurrence-of-giardia-cryptosporidium-and-entamoeba-in-wild-rhesus%C3%A2-macaques-macaca-mulatta-living-in-urban-and-semi-rural-north-west-india
#19
John J Debenham, Kristoffer Tysnes, Sandhya Khunger, Lucy J Robertson
Giardia duodenalis, Cryptosporidium spp., and Entamoeba spp. are intestinal protozoa capable of infecting a range of host species, and are important causes of human morbidity and mortality. Understanding their epidemiology is important, both for public health and for the health of the animals they infect. This study investigated the occurrence of these protozoans in rhesus macaques (Macaca mulatta) in India, with the aim of providing preliminary information on the potential for transmission of these pathogens between macaques and humans...
April 2017: International Journal for Parasitology. Parasites and Wildlife
https://www.readbyqxmd.com/read/28229042/an-investigation-of-parasitic-infections-and-review-of-molecular-characterization-of-the-intestinal-protozoa-in-nonhuman-primates-in-china-from-2009-to-2015
#20
Junqiang Li, Haiju Dong, Rongjun Wang, Fuchang Yu, Yayun Wu, Yankai Chang, Chenrong Wang, Meng Qi, Longxian Zhang
Parasites are a well-known threat to nonhuman primate (NHP) populations, and potentially cause zoonotic diseases in humans. In this study, the basic data was provided of the parasites in NHPs and the molecular characterization of the Enterocytozoon bieneusi, Giardia duodenalis, Cryptosporidium spp., and Entamoeba spp. were reviewed, which were found in these samples. A total of 3349 fecal samples were collected from 34 species reared at 17 districts in zoos, farms, free-range, or research laboratories, and examined microscopically...
April 2017: International Journal for Parasitology. Parasites and Wildlife
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