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https://www.readbyqxmd.com/read/27795362/novel-mirna-involved-in-host-response-to-avian-pathogenic-escherichia-coli-identified-by-deep-sequencing-and-integration-analysis
#1
Xinzheng Jia, Qinghua Nie, Xiquan Zhang, Lisa K Nolan, Susan J Lamont
Avian pathogenic Escherichia coli (APEC) causes one of the most common bacterial diseases of poultry worldwide. Effective control methods are, therefore, desirable and will be facilitated by a better understanding of the host response to the pathogen. Currently, miRNAs involved in host resistance to APEC are unknown. Here, we applied RNA sequencing to explore the changed miRNAs and deregulated genes in the spleen of broilers from non-challenged (NC), APEC challenged-mild pathology (CM), and APEC challenged-severe pathology (CS) groups...
October 17, 2016: Infection and Immunity
https://www.readbyqxmd.com/read/27738121/listeria-monocytogenes-infection-of-hd11-chicken-macrophage-like-cells
#2
N A Jarvis, J R Donaldson, C A O'Bryan, S C Ricke, P G Crandall
Listeria monocytogenes can be carried by and infect poultry, although the clinical disease in birds is rare. Escape from macrophage phagocytosis is a key step in pathogenesis for L. monocytogenes Therefore, we investigated the infection of the chicken macrophage-like cell line HD11 with 2 strains of L. monocytogenes EGD-e and Scott A. After infection, L. monocytogenes was quantified by spread plating and HD11 was quantified with trypan blue exclusion stain before enumeration. The standard macrophage killing protocols require washing the cell monolayers 3 times with PBS, which was found to negatively influence HD11 monolayers...
October 12, 2016: Poultry Science
https://www.readbyqxmd.com/read/27736938/heat-stress-and-lipopolysaccharide-stimulation-of-chicken-macrophage-like-cell-line-activates-expression-of-distinct-sets-of-genes
#3
Anna Slawinska, John C Hsieh, Carl J Schmidt, Susan J Lamont
Acute heat stress requires immediate adjustment of the stressed individual to sudden changes of ambient temperatures. Chickens are particularly sensitive to heat stress due to development of insufficient physiological mechanisms to mitigate its effects. One of the symptoms of heat stress is endotoxemia that results from release of the lipopolysaccharide (LPS) from the guts. Heat-related cytotoxicity is mitigated by the innate immune system, which is comprised mostly of phagocytic cells such as monocytes and macrophages...
2016: PloS One
https://www.readbyqxmd.com/read/27735192/influence-of-salmonella-enterica-serovar-pullorum-pathogenicity-island-2-on-type-iii-secretion-system-effetor-gene-expression-in-chicken-macrophage-hd11-cells
#4
Junlei Yin, Yun Chen, Xiaolei Xie, Jie Xia, Qiuchun Li, Shizhong Geng, Xinan Jiao
Salmonella pathogenicity island 2 (SPI2) can encode type III secretion system 2 (T3SS2) which plays an important role in systemic disease development through delivering different effector proteins into host cells. Here, the influence of Salmonella Pullorum pathogenicity island 2 on T3SS2 effetor gene expression was performed using qRT-PCR in chicken macrophage HD11 cells. Our results showed that all the detected genes (including pseudogenes sifB, sspH2 and steC) can express in HD11 cells of Salmonella Pullorum infection, deletion of SPI2 of Salmonella Pullorum did not significantly affect the expression of genes cigR, gtgA, slrP, sopD, sseK1, steB and steC, but had a significantly effect on the expression of genes pipB2, sifB, sopD2, sseJ, sseL, sspH2, steD, sifA, pipB and steA at different degrees...
October 13, 2016: Avian Pathology: Journal of the W.V.P.A
https://www.readbyqxmd.com/read/27565525/bioactive-extracts-from-berry-byproducts-on-the-pathogenicity-of-salmonella-typhimurium
#5
Serajus Salaheen, Ekta Jaiswal, Jungsoo Joo, Mengfei Peng, Ryan Ho, Danielle OConnor, Katrina Adlerz, Jose Helim Aranda-Espinoza, Debabrata Biswas
The aim of this study was to evaluate the phenotypic and genotypic properties of Salmonella enterica serovar Typhimurium (ST) in the presence of lethal and sublethal concentrations (SLC2LOG) of blackberry (Rubus fruticosus) and blueberry (Vaccinium corymbosum) pomace extracts. Antimicrobial susceptibility, physicochemical properties, motility, biofilm formation ability, virulence gene expression patterns, and the ability of ST to colonize in chick cecum were evaluated in the presence of these bioactive extracts...
November 21, 2016: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/27449156/initiation-and-regulation-of-immune-responses-to-immunization-with-whole-inactivated-vaccines-prepared-from-two-genetically-and-antigenically-distinct-lineages-of-egyptian-influenza-a-virus-subtype-h5n1
#6
Ahmed Samy, Mona I El-Enbaawy, Ahmed A El-Sanousi, Soad A Nasef, Hirokazu Hikono, Takehiko Saito
Following the introduction of highly pathogenic avian influenza (HPAI) virus subtype H5N1, the Egyptian government implemented a massive poultry vaccination campaign as the cornerstone of its policies to control the virus. The efficacy of vaccination has been evaluated primarily by measuring titers of antibodies inhibiting the hemagglutinating activity of the viral hemagglutinin (HA). However, other aspects of the host response remain poorly understood. In the present study, in addition to hemagglutination inhibition (HI) titers, cytokine profiles were examined and IFNγ concentrations were measured in vivo after immunization with a whole inactivated virus (WIV) prepared from a classical strain of clade 2...
October 2016: Archives of Virology
https://www.readbyqxmd.com/read/27325479/treatment-of-early-stage-unfavorable-hodgkin-lymphoma-efficacy-and-toxicity-of-4-versus-6-cycles-of-abvd-chemotherapy-with-radiation
#7
Jillian R Gunther, Michelle A Fanale, Jay P Reddy, Mani Akhtari, Grace L Smith, Chelsea C Pinnix, Sarah A Milgrom, Zeinab Abou Yehia, Pamela K Allen, Eleanor M Osborne, Osama Mawlawi, Bouthaina S Dabaja
PURPOSE: The German Hodgkin Study Group HD11 trial validated 4 cycles of doxorubicin, bleomycin, vinblastine, dacarbazine (ABVD) chemotherapy followed by involved field radiation therapy (IFRT) for early unfavorable Hodgkin lymphoma (HL) patients. However, practitioners often recommend 6 cycles followed by RT, especially for bulky disease. We compared patient outcomes after treatment with 4 or 6 cycles of ABVD followed by RT (IFRT and involved site RT [ISRT]). METHODS AND MATERIALS: We identified 128 patients treated for early unfavorable HL (GHSG criteria) between 2000 and 2013...
September 1, 2016: International Journal of Radiation Oncology, Biology, Physics
https://www.readbyqxmd.com/read/27163262/construction-and-characterization-of-a-cigr-deletion-mutant-of-salmonella-enterica-serovar-pullorum
#8
Junlei Yin, Jie Xia, Mingxin Tao, Lijuan Xu, Qiuchun Li, Shizhong Geng, Xinan Jiao
Salmonella enterica serovar Pullorum (S. Pullorum) is the causative agent of pullorum disease (PD) and results in severe economic losses to the poultry industry. As a Salmonella type III secretion system 2 (T3SS2) effector and predicted membrane protein, CigR is encoded by the cigR gene within Salmonella pathogenicity island 3 (SPI3). In order to research the influence of the cigR gene on S. Pullorum, a cigR mutant of S. Pullorum S06004 was constructed by the lambda Red recombination system, and then its characterization was analysed...
October 2016: Avian Pathology: Journal of the W.V.P.A
https://www.readbyqxmd.com/read/27141235/the-salmonella-pathogenicity-island-13-contributes-to-pathogenesis-in-streptomycin-pre-treated-mice-but-not-in-day-old-chickens
#9
Jacob R Elder, Kim Lam Chiok, Narayan C Paul, Gary Haldorson, Jean Guard, Devendra H Shah
BACKGROUND: Salmonella enterica serovar Enteritidis (S. Enteritidis) is a human and animal pathogen that causes gastroenteritis characterized by inflammatory diarrhea and occasionally an invasive systemic infection. Salmonella pathogenicity islands (SPIs) are horizontally acquired genomic segments known to contribute to Salmonella pathogenesis. The objective of the current study was to determine the contribution of SPI-13 to S. Enteritidis pathogenesis. METHODS: We deleted the entire SPI-13 (∆SPI-13) from the genome of S...
2016: Gut Pathogens
https://www.readbyqxmd.com/read/26848845/immunomodulatory-and-anti-inflammatory-activities-of-chicken-cathelicidin-2-derived-peptides
#10
Albert van Dijk, Mandy van Eldik, Edwin J A Veldhuizen, Hanne L M Tjeerdsma-van Bokhoven, Marcel R de Zoete, Floris J Bikker, Henk P Haagsman
Host Defence Peptides and derived peptides are promising classes of antimicrobial and immunomodulatory lead compounds. For this purpose we examined whether chicken cathelicidin-2 (CATH-2)-derived peptides modulate the function and inflammatory response of avian immune cells. Using a chicken macrophage cell line (HD11) we found that full-length CATH-2 dose-dependently induced transcription of chemokines CXCLi2/IL-8, MCP-3 and CCLi4/RANTES, but not of pro-inflammatory cytokine IL-1β. In addition, CATH-2 efficiently inhibited IL-1β and nitric oxide production by HD11 cells induced by different sources of lipopolysaccharides (LPS)...
2016: PloS One
https://www.readbyqxmd.com/read/26502907/fimbria-encoding-gene-yadc-has-a-pleiotropic-effect-on-several-biological-characteristics-and-plays-a-role-in-avian-pathogenic-escherichia-coli-pathogenicity
#11
Renu Verma, Thaís Cabrera Galvão Rojas, Renato Pariz Maluta, Janaína Luisa Leite, Livia Pilatti Mendes da Silva, Gerson Nakazato, Wanderley Dias da Silveira
The extraintestinal pathogen termed avian pathogenic Escherichia coli (APEC) is known to cause colibacillosis in chickens. The molecular basis of APEC pathogenesis is not fully elucidated yet. In this work, we deleted a component of the Yad gene cluster (yadC) in order to understand the role of Yad in the pathogenicity of the APEC strain SCI-07. In vitro, the transcription level of yadC was upregulated at 41°C and downregulated at 22°C. The yadC expression in vivo was more pronounced in lungs than in spleen, suggesting a role in the early steps of the infection...
January 2016: Infection and Immunity
https://www.readbyqxmd.com/read/26392283/role-of-chicken-melanoma-differentiation-associated-gene-5-in-induction-and-activation-of-innate-and-adaptive-immune-responses-to-infectious-bursal-disease-virus-in-cultured-macrophages
#12
Chih-Chun Lee, Ching Ching Wu, Tsang Long Lin
The objective of the present study was to determine if chicken melanoma-differentiation-associated gene 5 (MDA5) senses infectious bursal disease virus infection to induce innate immunity that bridges to adaptive immunity. During IBDV infection in HD11 cells, IBDV titers and RNA loads increased up to 3.4 × 10(7) plaque-forming units (PFU)/mL and 1114 ng/µL, respectively, at 24 hours postinfection (hpi). IBDV infection in HD11 cells induced significantly upregulated (p < 0.05) expression levels of chicken MDA5 (59-fold), interferon-β (IFN-β) (693-fold), dsRNA-dependent protein kinase (PKR) (4-fold), 2', 5'-oligoadenylate synthetase (OAS) (286-fold), myxovirus resistance gene (Mx) (22-fold), interleukin-1β (IL-1β) (5-fold), IL-6 (146-fold), IL-8 (4-fold), IL-10 (4-fold), inducible nitric oxide synthase (iNOS) (15-fold), and major histocompatibility complex class I (MHC class I) (4-fold)...
December 2015: Archives of Virology
https://www.readbyqxmd.com/read/26380970/phop-spi1-spi2-and-aroa-mutants-of-salmonella-enteritidis-induce-a-different-immune-response-in-chickens
#13
Marta Elsheimer-Matulova, Karolina Varmuzova, Kamila Kyrova, Hana Havlickova, Frantisek Sisak, Masudur Rahman, Ivan Rychlik
Poultry is the most frequent reservoir of non-typhoid Salmonella enterica for humans. Understanding the interactions between chickens and S. enterica is therefore important for vaccine design and subsequent decrease in the incidence of human salmonellosis. In this study we therefore characterized the interactions between chickens and phoP, aroA, SPI1 and SPI2 mutants of S. Enteritidis. First we tested the response of HD11 chicken macrophage-like cell line to S. Enteritidis infection monitoring the transcription of 36 genes related to immune response...
2015: Veterinary Research
https://www.readbyqxmd.com/read/26115276/effect-of-in-vitro-and-in-vivo-25-hydroxyvitamin-d-treatment-on-macrophages-t-cells-and-layer-chickens-during-a-coccidia-challenge
#14
RANDOMIZED CONTROLLED TRIAL
A Morris, R Shanmugasundaram, J McDonald, R K Selvaraj
This article describes the in vitro and in vivo effects of a 25-hydroxycholecalciferol (25[OH]D) treatment in layer hens during a mixed coccidia challenge. HD11 cells (chicken macrophage cell line) were treated in vitro with a coccidia antigen or in a medium supplemented with either 1,25-dihydroxycholecalciferol (1,25[OH]2D) or 25(OH)D. HD11 cells treated in vitro with 200 nM of 1,25(OH)2D had increased nitrite production (P < 0.01) compared with HD11 cells treated with 0 or 200 nM of 25(OH)D. Treating HD11 cells with 25(OH)D decreased IL-10 mRNA by 1...
June 2015: Journal of Animal Science
https://www.readbyqxmd.com/read/26046914/gene-expression-profiles-of-chicken-embryo-fibroblasts-in-response-to-salmonella-enteritidis-infection
#15
Ama Szmolka, Zoltán Wiener, Marta Elsheimer Matulova, Karolina Varmuzova, Ivan Rychlik
The response of chicken to non-typhoidal Salmonella infection is becoming well characterised but the role of particular cell types in this response is still far from being understood. Therefore, in this study we characterised the response of chicken embryo fibroblasts (CEFs) to infection with two different S. Enteritidis strains by microarray analysis. The expression of chicken genes identified as significantly up- or down-regulated (≥3-fold) by microarray analysis was verified by real-time PCR followed by functional classification of the genes and prediction of interactions between the proteins using Gene Ontology and STRING Database...
2015: PloS One
https://www.readbyqxmd.com/read/26024595/histone-deacetylase-hdac1-downregulates-transcription-of-the-serotonin-transporter-5-htt-gene-in-tumor-cells
#16
Daniel K Phi van, Eckhard Mühlbauer, Loc Phi-van
Serotonin (5-HT) has been reported to be involved in cancer progression by stimulating angiogenesis and cell growth. In this study, we examined the expression of the serotonin transporter (5-HTT) and the role of histone deacetylases (HDACs) in regulating the 5-HTT gene in tumor cells. The 5-HTT gene expression was almost silenced in chicken lymphoma DT40, myelomonocytic tumor HD11 and hepatoma DU249 cells, compared to their physiological counterpart. In contrast, HDAC1 mRNA expression was increased in these cell lines...
August 2015: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/25997976/equol-inhibits-lps-induced-oxidative-stress-and-enhances-the-immune-response-in-chicken-hd11-macrophages
#17
Zhongyong Gou, Shouqun Jiang, Chuntian Zheng, Zhimei Tian, Xiajing Lin
BACKGROUND/AIMS: There has been increasing recent attention on the antioxidative capacity of equol. This study tested the effect of equol on oxidative stress induced by lipopolysaccharide (LPS) and regulation of immunity in chicken macrophages. METHODS: Chicken HD11 macrophages were challenged with LPS (100 ng/mL) alone or with LPS (100 ng/mL) and (±)equol (10, 20, 40, 80, 160 μmol/L) together for 24h. Evaluated responses included the contents of malondialdehyde (MDA) and reduced glutathione (GSH), activities of total superoxide dismutase (T-SOD) and inducible nitric oxide synthase (iNOS), transcript abundance of superoxide dismutase 2 (SOD2), catalase (CAT), glutathione transferase (GST), Toll-like receptor 4 (TLR4), tumor necrosis factor alpha (TNFα) and interleukin-1 beta (IL-1β), and contents of the cytokines TNFα, IL-1β, interleukin-2 (IL-2) and interferon beta (IFNβ)...
2015: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/25938551/both-microrna-155-and-virus-encoded-mir-155-ortholog-regulate-tlr3-expression
#18
Xuming Hu, Jianqiang Ye, Aijian Qin, Haitao Zou, Hongxia Shao, Kun Qian
MicroRNA-155 (miR-155) has been as an important controller of TLR3 signalling. However, the interactions between miR-155 and TLR3 are poorly understood. Here, we focused on the regulation of the relationship between miR-155 and TLR3. Sequence analyses and firefly luciferase reporter assay revealed that miR-155 target were present in the coding sequences (CDS) of TLR3. And the expression of the TLR3 protein could be inhibited by a miR-155 mimic or by a virally encoded orthologue in chick embryo fibroblast cells...
2015: PloS One
https://www.readbyqxmd.com/read/25884204/differential-modulation-of-immune-response-and-cytokine-profiles-in-the-bursae-and-spleen-of-chickens-infected-with-very-virulent-infectious-bursal-disease-virus
#19
Mehdi Rasoli, Swee Keong Yeap, Sheau Wei Tan, Kiarash Roohani, Ye Wen Kristeen-Teo, Noorjahan Banu Alitheen, Yasmin Abd Rahaman, Ideris Aini, Mohd Hair Bejo, Pete Kaiser, Abdul Rahman Omar
BACKGROUND: Very virulent infectious bursal disease virus (vvIBDV) induces immunosuppression and inflammation in young birds, which subsequently leads to high mortality. In addition, infectious bursal disease (IBD) is one of the leading causes of vaccine failure on farms. Therefore, understanding the immunopathogenesis of IBDV in both the spleen and the bursae could help effective vaccine development. However, previous studies only profiled the differential expression of a limited number of cytokines, in either the spleen or the bursae of Fabricius of IBDV-infected chickens...
2015: BMC Veterinary Research
https://www.readbyqxmd.com/read/25863754/low-dose-consolidation-radiation-therapy-for-early-stage-unfavorable-hodgkin-lymphoma
#20
Jordan A Torok, Yuan Wu, Leonard R Prosnitz, Grace J Kim, Anne W Beaven, Louis F Diehl, Chris R Kelsey
PURPOSE: The German Hodgkin Study Group (GHSG) trial HD11 established 4 cycles of doxorubicin, bleomycin, vinblastine, and dacarbazine (ABVD) and 30 Gy of radiation therapy (RT) as a standard for early stage (I, II), unfavorable Hodgkin lymphoma (HL). Additional cycles of ABVD may allow for a reduction in RT dose and improved toxicity profile. METHODS AND MATERIALS: Patients treated with combined modality therapy at the Duke Cancer Institute for early stage, unfavorable HL by GHSG criteria from 1994 to 2012 were included...
May 1, 2015: International Journal of Radiation Oncology, Biology, Physics
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