Manuel A Ortega, Dillon P Cogan, Subha Mukherjee, Neha Garg, Bo Li, Gabrielle N Thibodeaux, Sonia I Maffioli, Stefano Donadio, Margherita Sosio, Jerome Escano, Leif Smith, Satish K Nair, Wilfred A van der Donk
Lantibiotics are ribosomally synthesized and post-translationally modified antimicrobial peptides containing thioether rings. In addition to these cross-links, the clinical candidate lantibiotic NAI-107 also possesses a C-terminal S-[(Z)-2-aminovinyl]-d-cysteine (AviCys) and a unique 5-chloro-l-tryptophan (ClTrp) moiety linked to its potent bioactivity. Bioinformatic and genetic analyses on the NAI-107 biosynthetic gene cluster identified mibH and mibD as genes encoding flavoenzymes responsible for the formation of ClTrp and AviCys, respectively...
February 17, 2017: ACS Chemical Biology