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phosphatidyl serin+treatment

Majid Mahdavi, Malihe Mohseni Lavi, Reza Yekta, Mohammad Amin Moosavi, Mahnaz Nobarani, Saeed Balalei, Sanam Arami, Mohammad Reza Rashidi
Previous studies have suggested that quinazolinone derivatives are potent apoptosis-inducing agents in various cancer cell lines. In the present study, we have investigated cytotoxic, apoptosis induction, and molecular docking activities of the spiroquinazolinone benzamide derivatives family on MCF-7 human breast cancer cells. The MTT cytotoxicity assays and docking studies showed that 4t-CHQB was the most active compound among the prepared spiroquinazolinone benzamide compounds with IC50 of 50 ± 1.2 μM and was selected for further assessments...
October 3, 2016: Chemico-biological Interactions
Nirmala Jagadish, Namita Gupta, Sumit Agarwal, Deepak Parashar, Aditi Sharma, Rukhsar Fatima, Amos Prashant Topno, Vikash Kumar, Anil Suri
Recently, we demonstrated the association of sperm-associated antigen 9 (SPAG9) expression with breast cancer. Among breast cancer, 15 % of the cancers are diagnosed as triple-negative breast cancers (TNBC) based on hormone receptor status and represent an important clinical challenge because of lack of effective available targeted therapy. Therefore, in the present investigation, plasmid-based small hairpin (small hairpin RNA (shRNA)) approach was used to ablate SPAG9 in aggressive breast cancer cell line model (MDA-MB-231) in order to understand the role of SPAG9 at molecular level in apoptosis, cell cycle, and epithelial-to-mesenchymal transition (EMT) signaling...
July 23, 2016: Tumour Biology: the Journal of the International Society for Oncodevelopmental Biology and Medicine
F J Scariot, L M Jahn, J P Maianti, A P L Delamare, S Echeverrigaray
Mancozeb (MZ), a mixture of ethylene-bis-dithiocarbamate manganese and zinc salts, is one of the most widely used fungicides in agriculture. Toxicologic studies in mammals and mammalian cells indicate that this fungicide can cause neurological and cytological disorders, putatively associated with pro-oxidant and apoptotic effects. Yeast adaptation to sub-inhibitory concentrations of MZ has been correlated with oxidative response, proteins degradation, and energy metabolism, and its main effect on yeast has been attributed to its high reactivity with thiol groups in proteins...
July 2016: Apoptosis: An International Journal on Programmed Cell Death
Malabika Banerjee, Subrata Chattopadhyay, Tathagata Choudhuri, Rammohan Bera, Sanjay Kumar, Biswajit Chakraborty, Samir Kumar Mukherjee
BACKGROUND: Breast cancer is considered as an increasing major life-threatening concern among the malignancies encountered globally in females. Traditional therapy is far from satisfactory due to drug resistance and various side effects, thus a search for complementary/alternative medicines from natural sources with lesser side effects is being emphasized. Andrographis paniculata, an oriental, traditional medicinal herb commonly available in Asian countries, has a long history of treating a variety of diseases, such as respiratory infection, fever, bacterial dysentery, diarrhea, inflammation etc...
2016: Journal of Biomedical Science
Jesmin Mondal, Asmita Samadder, Anisur Rahman Khuda-Bukhsh
OBJECTIVE: To provide in vitro evidence of Psorinum treatment against cancer cells in a controlled study. METHODS: Effects of homeopathic Psorinum 6× on cell viability were initially determined in several cancer cell lines, including A549, HepG2 and MCF-7, using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, and an ethanol 6× control. The cell line that exhibited highest inhibition was selected and used in the following experiments. A range of Psorinum 6× doses was used to explore treatment effects on cell cycle arrest, cell death (apoptosis), generation of reactive oxygen species (ROS) and change in mitochondrial membrane potential (MMP) using flow cytometry and fluorescence microscopy, respectively...
March 2016: Journal of Integrative Medicine
Josephraj Sophia, Kranthi Kiran Kishore T, Jaganathan Kowshik, Rajakishore Mishra, Siddavaram Nagini
Glycogen synthase kinase-3β (GSK-3β), a serine/threonine kinase is frequently inactivated by the oncogenic signalling kinases PI3K/Akt and MAPK/ERK in diverse malignancies. The present study was designed to investigate GSK-3β signalling circuits in the 7,12-dimethylbenz[a]anthracene (DMBA)-induced hamster buccal pouch (HBP) carcinogenesis model and the therapeutic potential of the neem limonoid nimbolide. Inactivation of GSK-3β by phosphorylation at serine 9 and activation of PI3K/Akt, MAPK/ERK and β-catenin was associated with increased cell proliferation and apoptosis evasion during stepwise evolution of HBP carcinomas...
2016: Scientific Reports
Shelly Gupta, G V R Krishna Prasad, Arunika Mukhopadhaya
Porins, a major class of outer membrane proteins in Gram-negative bacteria, primarily act as transport channels. OmpU is one of the major porins of human pathogen, Vibrio cholerae. In the present study, we show that V. cholerae OmpU has the ability to induce target cell death. Although OmpU-mediated cell death shows some characteristics of apoptosis, such as flipping of phosphatidylserine in the membrane as well as cell size shrinkage and increased cell granularity, it does not show the caspase-3 activation and DNA laddering pattern typical of apoptotic cells...
December 25, 2015: Journal of Biological Chemistry
Yan Zhang, Ming-xue Zheng, Zhi-yong Xu, Huan-cheng Xu, Xiao-zhen Cui, Sha-sha Yang, Wen-long Zhao, Shan Li, Qiang-hua Lv, Rui Bai
Coccidiosis causes considerable economic losses in the poultry industry. At present, the pathology of coccidiosis is preventable with anticoccidials and vaccination, although at considerable cost to the international poultry industry. The purpose of the present study was to elucidate the relationship between Eimeria tenella development and host cell apoptosis in chickens, which provides a theoretical basis for further study of the injury mechanism of E. tenella and the prevention and treatment of coccidiosis...
December 2015: Poultry Science
A-Rum Yoon, Jinwoo Hong, Chae-Ok Yun
Utility of traditional oncolytic adenovirus (Ad) has been limited due to low expression of coxsackie and adenovirus receptor (CAR) in cancer cells which results in poor infectivity of Ads. Here with an aim of improving the efficiency of Ad's entry to the cell, we generated a novel tropism-expanded oncolytic Ad which contains the epitope of vesicular stomatitis virus glycoprotein (VSVG) at the HI-loop of Ad fiber. We generated 9 variants of oncolytic Ads with varying linkers and partial deletion to the fiber...
October 27, 2015: Oncotarget
Desmond N Jackson, Lin Yang, ShiBiao Wu, Edward J Kennelly, Peter N Lipke
Xanthochymol and garcinol, isoprenylated benzophenones purified from Garcinia xanthochymus fruits, showed multiple activities against Candida albicans biofilms. Both compounds effectively prevented emergence of fungal germ tubes and were also cytostatic, with MICs of 1 to 3 μM. The compounds therefore inhibited development of hyphae and subsequent biofilm maturation. Xanthochymol treatment of developing and mature biofilms induced cell death. In early biofilm development, killing had the characteristics of apoptosis, including externalization of phosphatidyl serine and DNA fragmentation, as evidenced by terminal deoxynucleotidyltransferase-mediated dUTP-biotin nick end labeling (TUNEL) fluorescence...
October 2015: Antimicrobial Agents and Chemotherapy
Majid Ghasemian, Majid Mahdavi, Payman Zare, Mohammad Ali Hosseinpour Feizi
Spiroquinazolinone compounds have been considered as a new series of potent apoptosis-inducing agents. In this study, anti-proliferative and apoptotic effects of the derivatives from the spiroquinazolinone family were investigated in the human chronic myeloid leukemia K562 cells. The K562 cells were treated with various concentrations of the spiroquinazolinone (10-300 µM) for 3 days and cell viability was determined by MTT growth inhibition assay. 4t-QTC was more active among these compounds with IC50 of 50 ± 3...
February 2015: Journal of Toxicological Sciences
Sivanesan Dhandayuthapani, Hasan Azad, Appu Rathinavelu
Tulsi (Ocimum sanctum Linn), commonly known as "holy basil," has been used for the treatment of a wide range of ailments in many parts of the world. This study focuses on apoptosis-inducing ability of tulsi extract on prostate cancer cells. For this purpose LNCaP prostate cancer cells were treated with different concentrations of 70% ethanolic extract of tulsi (EET) and then the cytotoxicity was determined after 24 and 48 h. After treatment with EET externalization of phosphatidyl serine (PS) from the inner membrane to outer leaflet of the plasma membrane was clearly evidenced by the results obtained from both flow cytometry analysis with Annexin V-FITC and pSIVA-IANBD binding fluorescence microscopy assay...
July 2015: Journal of Medicinal Food
Wilfredo Quiñones, Ana J Cáceres, Maria Tibisay Ruiz, Juan Luis Concepción
Constituents of the glycosomal membrane from Leishmania mexicana should play a critical role in the coordination of metabolic processes occurring in the cytosol and those compartmentalized within glycosomes. We have made an inventory of glycosomal membrane-associated proteins using approaches specific for enriching both integral and peripheral membrane proteins. Surprisingly, 70% of the proteins were recovered in the hydrophobic fraction of membranes solubilized with Triton X-114, while 20% were present in the soluble fraction obtained upon treatment with Na2CO3...
April 2015: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
Jun-O Jin, Qing Yu
Although some immune modulatory effects of fucoidan have been elucidated, the effects of fucoidan on the apoptosis and activation of human neutrophils have not been investigated. In this study, we demonstrated that fucoidan purified from the brown seaweed Undaria pinnatifilda delays spontaneous apoptosis of human neutrophils and induces their activation. Fucoidan treatment inhibited apoptotic nuclei changes and phosphatidyl serine (PS) exposure on neutrophils cultured in vitro for 24h. The delay in neutrophil apoptosis mediated by fucoidan was associated with increased levels of the anti-apoptotic protein Mcl-1 and decreased levels of activated caspase-3...
February 2015: International Journal of Biological Macromolecules
Pamela Ovadje, Dennis Ma, Phillip Tremblay, Alessia Roma, Matthew Steckle, Jose-Antonio Guerrero, John Thor Arnason, Siyaram Pandey
BACKGROUND: Currently chemotherapy is limited mostly to genotoxic drugs that are associated with severe side effects due to non-selective targeting of normal tissue. Natural products play a significant role in the development of most chemotherapeutic agents, with 74.8% of all available chemotherapy being derived from natural products. OBJECTIVE: To scientifically assess and validate the anticancer potential of an ethanolic extract of the fruit of the Long pepper (PLX), a plant of the piperaceae family that has been used in traditional medicine, especially Ayurveda and investigate the anticancer mechanism of action of PLX against cancer cells...
2014: PloS One
Jesmin Mondal, Ashis Kumar Panigrahi, Anisur Rahman Khuda-Bukhsh
OBJECTIVE: Conium maculatum extract is used as a traditional medicine for cervix carcinoma including homeopathy. However, no systematic work has so far been carried out to test its anti-cancer potential against cervix cancer cells in vitro. Thus, in this study, we investigated whether ethanolic extract of conium is capable of inducing cytotoxicity in different normal and cancer cell lines including an elaborate study in HeLa cells. MATERIALS AND METHODS: Conium's effects on cell cycle, reactive oxygen species (ROS) accumulation, mitochondrial membrane potential (MMP) and apoptosis, if any, were analyzed through flow cytometry...
August 2014: Pharmacognosy Magazine
G Mátis, A Kulcsár, V Turowski, H Fébel, Zs Neogrády, K Huber
The influence of butyrate on insulin signaling in chickens was studied because butyrate is produced during microbial fermentation in the large intestine of birds, and butyrate is widely used as a feed additive in animal production. Ross 308 broiler chickens received a daily intraingluvial bolus of sodium butyrate (0.25 g/kg body weight) on days 20-24 of life (n = 10). Plasma butyrate concentration increased after receiving oral butyrate treatment (P < 0.001). Oral butyrate application was associated with decreased protein expression of insulin receptor β subunit (IRβ) in liver (P = 0...
January 2015: Domestic Animal Endocrinology
Shreyasi Palit, Susanta Kar, Gunjan Sharma, Pijush K Das
Hesperetin, a flavanone glycoside predominantly found in citrus fruits, exhibits a wide array of biological properties. In the present study hesperetin exhibited a significant cytotoxic effect in human breast carcinoma MCF-7 cells in a concentration- and time-dependent manner without affecting normal (HMEC) as well as immortalized normal mammary epithelial cells (MCF-10A). The cytotoxic effect of hesperetin was due to the induction of apoptosis as evident from the phosphatidyl-serine externalization, DNA fragmentation, caspase-7 activation, and PARP cleavage...
August 2015: Journal of Cellular Physiology
Radhika Kapoor, Shruti Singh, Madhulika Tripathi, Priyanka Bhatnagar, Poonam Kakkar, Kailash Chand Gupta
Nanotized phytochemicals are being explored by researchers for promoting their uptake and effectiveness at lower concentrations. In this study, O-hexadecyl-dextran entrapped berberine chloride nanoparticles (BC-HDD NPs) were prepared, and evaluated for their cytoprotective efficacy in high glucose stressed primary hepatocytes and the results obtained compared with bulk berberine chloride (BBR) treatment. The nanotized formulation treated primary hepatocytes that were exposed to high glucose (40 mM), showed increased viability compared to the bulk BBR treated cells...
2014: PloS One
S J Coughlan, R G Wyn Jones
In secondary leaves from spinach plants pretreated in vermiculite for 24 h with 300 mM NaCl, glycinebetaine accumulated at a rate of circa 0.16 μmol 100 μg(-1) Chl d(-1) (2 μmol g(-1) FW d(-1)), about three times the rate of control plants. The soluble carbohydrate and free amino acid contents did not increase significantly following salinisation until after 4 d when the relative growth rate also decreased. Leaf proline levels remained very low throughout the experimental period. K(+) on a tissue water basis remained constant at 200 mM while Cl(-) and Na(+) levels increased linearly to reach 175 and 100 mM respectively after 5 d of saline treatment...
March 1982: Planta
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