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https://www.readbyqxmd.com/read/29161268/nucleic-acid-purification-from-plants-animals-and-microbes-in-under-30-seconds
#1
Yiping Zou, Michael Glenn Mason, Yuling Wang, Eugene Wee, Conny Turni, Patrick J Blackall, Matt Trau, Jose Ramon Botella
Nucleic acid amplification is a powerful molecular biology tool, although its use outside the modern laboratory environment is limited due to the relatively cumbersome methods required to extract nucleic acids from biological samples. To address this issue, we investigated a variety of materials for their suitability for nucleic acid capture and purification. We report here that untreated cellulose-based paper can rapidly capture nucleic acids within seconds and retain them during a single washing step, while contaminants present in complex biological samples are quickly removed...
November 2017: PLoS Biology
https://www.readbyqxmd.com/read/29159711/treatment-of-richter-s-syndrome
#2
REVIEW
Adalgisa Condoluci, Davide Rossi
Based on the available literature, mostly derived from retrospective or non-randomized phase I or II studies, it is difficult to define an optimized treatment approach for patients developing Richter's syndrome (RS). Early recognition of chronic lymphocytic leukemia (CLL) patients presenting clinical features suspected for a transformation is useful to avoid exposing them to multiple lines of therapy that, being targeted to CLL progression, have poor efficacy against RS. Because of the low specificity (~ 50-60%) of clinical signs of RS (such as rapid and discordant bulky localized lymphadenopathies, elevated LDH levels, emergent physical deterioration, and/or fever in the absence of infection), a (18)FDG PET/CT and a biopsy are recommended to confirm RS...
November 21, 2017: Current Treatment Options in Oncology
https://www.readbyqxmd.com/read/29159273/a-health-economic-evaluation-of-needle-based-confocal-laser-endomicroscopy-for-the-diagnosis-of-pancreatic-cysts
#3
Claude Le Pen, Laurent Palazzo, Bertrand Napoléon
Background and study aims:  The low sensitivity of endoscopic ultrasound-guided fine-needle aspiration (EUS-FNA), especially for the diagnosis of serous cystadenomas (SCAs), can be associated with diagnostic uncertainty that can regularly lead to unnecessary surgical procedures. Needle-based confocal laser endomicroscopy (nCLE) used with EUS-FNA improves diagnostic accuracy, helping to reduce unnecessary surgery and patient follow-up. This study was conducted to evaluate the economic benefit of EUS-FNA + nCLE...
October 2017: Endoscopy International Open
https://www.readbyqxmd.com/read/29158552/care-and-cost-consequences-of-pediatric-whole-genome-sequencing-compared-to-chromosome-microarray
#4
Robin Z Hayeems, Jasmin Bhawra, Kate Tsiplova, M Stephen Meyn, Nasim Monfared, Sarah Bowdin, D James Stavropoulos, Christian R Marshall, Raveen Basran, Cheryl Shuman, Shinya Ito, Iris Cohn, Courtney Hum, Marta Girdea, Michael Brudno, Ronald D Cohn, Stephen W Scherer, Wendy J Ungar
The clinical use of whole-genome sequencing (WGS) is expected to alter pediatric medical management. The study aimed to describe the type and cost of healthcare activities following pediatric WGS compared to chromosome microarray (CMA). Healthcare activities prompted by WGS and CMA were ascertained for 101 children with developmental delay over 1 year. Activities following receipt of non-diagnostic CMA were compared to WGS diagnostic and non-diagnostic results. Activities were costed in 2016 Canadian dollars (CDN)...
November 20, 2017: European Journal of Human Genetics: EJHG
https://www.readbyqxmd.com/read/29155763/quantifying-microorganisms-at-low-concentrations-using-digital-holographic-microscopy-dhm
#5
Manuel Bedrossian, Casey Barr, Chris A Lindensmith, Kenneth Nealson, Jay L Nadeau
Accurately detecting and counting sparse bacterial samples has many applications in the food, beverage, and pharmaceutical processing industries, in medical diagnostics, and for life detection by robotic missions to other planets and moons of the solar system. Currently, sparse bacterial samples are counted by culture plating or epifluorescence microscopy. Culture plates require long incubation times (days to weeks), and epifluorescence microscopy requires extensive staining and concentration of the sample...
November 1, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29153368/diagnostic-value-of-cone-beam-computed-tomography-cbct-in-occult-scaphoid-and-wrist-fractures
#6
Christophe Borel, Ahmed Larbi, Stephanie Delclaux, Franck Lapegue, Helene Chiavassa-Gandois, Nicolas Sans, Marie Faruch-Bilfeld
OBJECTIVE: Evaluate the diagnostic value of cone beam computed tomography (CBCT) for scaphoid and wrist fractures that are missed on standard radiographs. MATERIALS AND METHODS: Between September 2014 and October 2015, we prospectively enrolled 49 patients with a clinically suspected scaphoid fracture following an acute injury but had normal radiographs. Each patients underwent radiographs, CBCT and (magnetic resonance imaging) MRI within 7days of the initial injury event...
December 2017: European Journal of Radiology
https://www.readbyqxmd.com/read/29153075/is-whole-colon-investigation-by-colonoscopy-computerised-tomography-colonography-or-barium-enema-necessary-for-all-patients-with-colorectal-cancer-symptoms-and-for-which-patients-would-flexible-sigmoidoscopy-suffice-a-retrospective-cohort-study
#7
Wendy Atkin, Kate Wooldrage, Urvi Shah, Kate Skinner, Jeremy P Brown, Willie Hamilton, Ines Kralj-Hans, Michael R Thompson, Karen G Flashman, Steve Halligan, Siwan Thomas-Gibson, Margaret Vance, Amanda J Cross
BACKGROUND: For patients referred to hospital with suspected colorectal cancer (CRC), it is current standard clinical practice to conduct an examination of the whole colon and rectum. However, studies have shown that an examination of the distal colorectum using flexible sigmoidoscopy (FS) can be a safe and clinically effective investigation for some patients. These findings require validation in a multicentre study. OBJECTIVES: To investigate the links between patient symptoms at presentation and CRC risk by subsite, and to provide evidence of whether or not FS is an effective alternative to whole-colon investigation (WCI) in patients whose symptoms do not suggest proximal or obstructive disease...
November 2017: Health Technology Assessment: HTA
https://www.readbyqxmd.com/read/29152734/access-to-diagnostic-and-therapeutic-facilities-for-psychogenic-nonepileptic-seizures-an-international-survey-by-the-ilae-pnes-task-force-2nd-revision
#8
Coraline Hingray, Wissam El-Hage, Rod Duncan, David Gigineishvili, Kousuke Kanemoto, W Curt LaFrance, Alejandro de Marinis, Ravi Paul, Chrisma Pretorius, José F Téllez Zenteno, Hannah Wiseman, Markus Reuber
OBJECTIVE: Studies from a small number of countries suggest that patients with psychogenic nonepileptic seizures (PNES) have limited access to diagnostic and treatment services. The PNES Task Force of the International League Against Epilepsy (ILAE) carried out 2 surveys to explore the diagnosis and treatment of PNES around the world. METHODS: A short survey (8 questions) was sent to all 114 chapters of the ILAE. A longer survey (36 questions) was completed by healthcare professionals who see patients with seizures...
November 20, 2017: Epilepsia
https://www.readbyqxmd.com/read/29149993/label-free-determination-of-prostate-specific-membrane-antigen-in-human-whole-blood-at-nanomolar-levels-by-magnetically-assisted-surface-enhanced-raman-spectroscopy
#9
Zuzana Chaloupková, Anna Balzerová, Jitka Bařinková, Zdenka Medříková, Pavel Šácha, Petr Beneš, Václav Ranc, Jan Konvalinka, Radek Zbořil
Prostate cancer is one of the most common cancers among men and can in its later stages cause serious medical problems. Due to the limited suitability of current diagnostic biochemical markers, new biomarkers for the detection of prostate cancer are highly sought after. An ideal biomarker should serve as a reliable prognostic marker, be applicable for early diagnosis, and be applicable for monitoring of therapeutic response. One potential candidate is glutamate carboxypeptidase II (GCPII), also known as prostate specific membrane antigen (PSMA), which has a promising role for direct imaging...
January 2, 2018: Analytica Chimica Acta
https://www.readbyqxmd.com/read/29146902/single-molecule-multiplexed-nanopore-protein-screening-in-human-serum-using-aptamer-modified-dna-carriers
#10
Jasmine Y Y Sze, Aleksandar P Ivanov, Anthony E G Cass, Joshua B Edel
The capability to screen a range of proteins at the single-molecule level with enhanced selectivity in biological fluids has been in part a driving force in developing future diagnostic and therapeutic strategies. The combination of nanopore sensing and nucleic acid aptamer recognition comes close to this ideal due to the ease of multiplexing, without the need for expensive labelling methods or extensive sample pre-treatment. Here, we demonstrate a fully flexible, scalable and low-cost detection platform to sense multiple protein targets simultaneously by grafting specific sequences along the backbone of a double-stranded DNA carrier...
November 16, 2017: Nature Communications
https://www.readbyqxmd.com/read/29144379/a-digital-microfluidics-platform-for-loop-mediated-isothermal-amplification-detection
#11
Beatriz Jorge Coelho, Bruno Veigas, Hugo Águas, Elvira Fortunato, Rodrigo Martins, Pedro Viana Baptista, Rui Igreja
Digital microfluidics (DMF) arises as the next step in the fast-evolving field of operation platforms for molecular diagnostics. Moreover, isothermal schemes, such as loop-mediated isothermal amplification (LAMP), allow for further simplification of amplification protocols. Integrating DMF with LAMP will be at the core of a new generation of detection devices for effective molecular diagnostics at point-of-care (POC), providing simple, fast, and automated nucleic acid amplification with exceptional integration capabilities...
November 16, 2017: Sensors
https://www.readbyqxmd.com/read/29143620/optimising-diagnosis-of-viraemic-hepatitis-c-infection-the-development-of-a-target-product-profile
#12
Elena Ivanova Reipold, Philippa Easterbrook, Alessandra Trianni, Nivedha Panneer, Douglas Krakower, Stefano Ongarello, Teri Roberts, Veronica Miller, Claudia Denkinger
BACKGROUND: The current low access to virological testing to confirm chronic viraemic HCV infection in low- and middle-income countries (LMIC) is limiting the rollout of hepatitis C (HCV) care. Existing tests are complex, costly and require sophisticated laboratory infrastructure. Diagnostic manufacturers need guidance on the optimal characteristics a virological test needs to have to ensure the greatest impact on HCV diagnosis and treatment in LMIC. Our objective was to develop a target product profile (TPP) for diagnosis of HCV viraemia using a global stakeholder consensus-based approach...
November 1, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/29143611/one-or-two-serological-assay-testing-strategy-for-diagnosis-of-hbv-and-hcv-infection-the-use-of-predictive-modelling
#13
John V Parry, Philippa Easterbrook, Anita R Sands
BACKGROUND: Initial serological testing for chronic hepatitis B virus (HBV) and hepatitis C virus (HCV) infection is conducted using either rapid diagnostic tests (RDT) or laboratory-based enzyme immunoassays (EIA)s for detection of hepatitis B surface antigen (HBsAg) or antibodies to HCV (anti-HCV), typically on serum or plasma specimens and, for certain RDTs, capillary whole blood. WHO recommends the use of standardized testing strategies - defined as a sequence of one or more assays to maximize testing accuracy while simplifying the testing process and ideally minimizing cost...
November 1, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/29143368/re-thinking-the-role-of-radiometal-isotopes-towards-a-future-concept-for-theranostic-radiopharmaceuticals
#14
Johannes Notni, Hans-Jürgen Wester
The potential and future role of certain metal radionuclides, for example, (44) Sc, (89) Zr, (86) Y, (64) Cu, (68) Ga, (177) Lu, (225) Ac, (213) Bi, and several terbium isotopes has been controversely discussed in the past decades. Furthermore, the possible benefits of "matched pairs" of isotopes for tandem applications of diagnostics and therapeutics (theranostics) have been emphasized, while such approaches still haven't made their way into routine clinical practice. Analysis of bibliographical data illustrates how popularity of certain nuclides has been promoted by cycles of availability and applications...
November 16, 2017: Journal of Labelled Compounds & Radiopharmaceuticals
https://www.readbyqxmd.com/read/29142796/impact-of-depression-on-hospitalization-and-related-outcomes-for-parkinson-s-disease-patients-a-nationwide-inpatient-sample-based-retrospective-study
#15
Rikinkumar S Patel, Ramkrishna Makani, Zeeshan Mansuri, Upenkumar Patel, Rupak Desai, Amit Chopra
Background Major Depressive Disorder (MDD) is a common comorbidity that significantly affects the quality of life and disease outcomes in Parkinson's disease (PD) patients. No studies have been conducted to our knowledge to address the health care utilization and its outcomes in these patients. The aim of this study is to analyze and discern the differences in the hospitalization outcomes, comorbid conditions, and utilization of procedures in PD patients versus patients with comorbid MDD. Methods We used the Nationwide Inpatient Sample from the Healthcare Cost and Utilization Project from year's 2010-2014...
September 3, 2017: Curēus
https://www.readbyqxmd.com/read/29140309/biosensors-to-diagnose-chagas-disease-a-brief-review
#16
REVIEW
María-Isabel Rocha-Gaso, Luis-Jesús Villarreal-Gómez, Denis Beyssen, Frédéric Sarry, Marco-Antonio Reyna, Carlos-Napoleón Ibarra-Cerdeña
Chagas disease (CD), which mostly affects those living in deprived areas, has become one of Latin America's main public health problems. Effective prevention of the disease requires early diagnosis, initiation of therapy, and regular blood monitoring of the infected individual. However, the majority of the Trypanosoma cruzi infections go undiagnosed because of mild symptoms, limited access to medical attention and to a high variability in the sensitivity and specificity of diagnostic tests. Consequently, more affordable and accessible detection technologies capable of providing early diagnosis and T...
November 15, 2017: Sensors
https://www.readbyqxmd.com/read/29136911/one-step-detection-for-two-serological-biomarker-species-to-improve-the-diagnostic-accuracy-of-hepatocellular-carcinoma
#17
Tao Gao, Jun Zhi, Chaoli Mu, Shiyu Gu, Jing Xiao, Jie Yang, Zhaoxia Wang, Yang Xiang
At present, the accuracy of clinical hepatocellular carcinoma (HCC) diagnosis needs to be further improved. In this work, two kinds of serological biomarker species, microRNA and protein biomarker, have been detected simultaneously to identify HCC. Herein, a dual-aptamer hairpin DNA oligonucleotide is designed as the electrochemical sensing probe (ESP) to achieve this goal. The hairpin-structured DNA probe consists microRNA-16 (miR-16) complementary sequence and alpha fetoprotein (AFP) aptamer sequence, so it can both capture miR-16 and AFP...
February 1, 2018: Talanta
https://www.readbyqxmd.com/read/29136589/nucleic-acid-aptamer-based-methods-for-diagnosis-of-infections
#18
REVIEW
Ki Soo Park
Infectious diseases are a serious global problem, which not only take an enormous human toll but also incur tremendous economic losses. In combating infectious diseases, rapid and accurate diagnostic tests are required for pathogen identification at the point of care (POC). In this review, investigations of diagnostic strategies for infectious diseases that are based on aptamers, especially nucleic acid aptamers, oligonucleotides that have high affinities and specificities toward their targets, are described...
November 8, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29135619/the-heart-pathway-and-hospital-cost-savings
#19
Amy A Yau, Long T Nguyendo, LaKeesha L Lockett, Edward Michaud
Chest pain is a common complaint in emergency departments. Several guidelines and tools exist to help the clinician determine need for hospitalization. For low-risk patients, clinical judgment can underestimate a patient's risk of major adverse cardiac event. Implementation of an advanced diagnostic protocol with the HEART Pathway can reduce hospital cost. For our academic institution, we saw an approximate $1 million in total savings during the initial implementation year along with increased outpatient visits...
December 2017: Critical Pathways in Cardiology
https://www.readbyqxmd.com/read/29135109/18f-fdg-pet-ct-versus-99tcm-mibi-spect-which-is-better-for-detection-of-solitary-pulmonary-nodules
#20
Guoliang Xia, Chuanguo An, Zonghua Ming, Hong Guo, Lihong Liu, Yufeng Li
PURPOSE: To compare the diagnostic value of 99Tcm-MIBI- SPECT and 18F-FDG-PET/CT in differentiating benign from malignant solitary pulmonary nodules (SPNs). METHODS: 170 SPNs were involved in this study (78 with 99Tcm-MIBI-SPECT and 92 with 18F-FDG-PET/CT). Definite diagnosis of SPNs was determined by biopsy. The diagnostic efficiency of 99Tcm-MIBI-SPECT and 18F-FDGPET/ CT in differentiating benign from malignant SPNs was analyzed and compared. RESULTS: Seventy-eight patients with SPNs were examined with 99Tcm-MIBI-SPECT (26 with malignant SPNs and 52 with benign SPNs)...
September 2017: Journal of B.U.ON.: Official Journal of the Balkan Union of Oncology
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