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https://www.readbyqxmd.com/read/28432659/current-role-of-blood-and-urine-biomarkers-in-the-clinical-care-of-adults-with-congenital-heart-disease
#1
REVIEW
Saurabh Rajpal, Laith Alshawabkeh, Alexander R Opotowsky
PURPOSE OF REVIEW: There is an increasing number of adult patients with congenital heart disease (CHD). While several biomarkers have been validated and integrated into general cardiology clinical practice, these tests are often applied to adults with CHD in the absence of disease-specific validation. Although these patients are often grouped into a single population, there is heterogeneous pathophysiology, variable disease chronicity, extensive multisystem involvement, and a low event rate relative to acquired heart disease...
June 2017: Current Cardiology Reports
https://www.readbyqxmd.com/read/28426961/endogenous-piezo1-can-confound-mechanically-activated-channel-identification-and-characterization
#2
Adrienne E Dubin, Swetha Murthy, Amanda H Lewis, Lucie Brosse, Stuart M Cahalan, Jörg Grandl, Bertrand Coste, Ardem Patapoutian
A gold standard for characterizing mechanically activated (MA) currents is via heterologous expression of candidate channels in naive cells. Two recent studies described MA channels using this paradigm. TMEM150c was proposed to be a component of an MA channel partly based on a heterologous expression approach (Hong et al., 2016). In another study, Piezo1's N-terminal "propeller" domain was proposed to constitute an intrinsic mechanosensitive module based on expression of a chimera between a pore-forming domain of the mechanically insensitive ASIC1 channel and Piezo1 (Zhao et al...
April 19, 2017: Neuron
https://www.readbyqxmd.com/read/28426178/multiplexing-n-glycan-analysis-by-dna-analyzer
#3
Hua-Tao Feng, Pingjing Li, Guo Rui, James Stray, Shaheer Khan, Shiaw-Min Chen, Sam F Y Li
Analysis of N-glycan structures has been gaining attentions over the years due to their critical importance to biopharma based applications and growing roles in biological research. Glycan profiling is also critical to the development of biosimilar drugs. The detailed characterization of N-glycosylation is mandatory because it is a non-template driven process and that significantly influences critical properties such as bio-safety and bio-activity. The ability to comprehensively characterize highly complex mixtures of N-glycans has been analytically challenging and stimulating because of the difficulties in both the structure complexity and time-consuming sample pretreatment procedures...
April 20, 2017: Electrophoresis
https://www.readbyqxmd.com/read/28424615/huangqin-decoction-attenuates-cpt-11-induced-gastrointestinal-toxicity-by-regulating-bile-acids-metabolism-homeostasis
#4
Xu Wang, Dong-Ni Cui, Xiao-Min Dai, Jing Wang, Wei Zhang, Zun-Jian Zhang, Feng-Guo Xu
Irinotecan (CPT-11) is a potent chemotherapeutic agent, however, its clinical usage is often limited by the induction of severe gastrointestinal (GI) toxicity, especially late-onset diarrhea. HuangQin Decoction (HQD), commonly used for the treatment of GI ailments, has been proved could significantly ameliorate the intestinal toxicity of CPT-11. To reveal the mechanisms of CPT-11-induced toxicity and the modulation effects of HQD, a previous untargeted metabolomics study was performed and the results indicated that HQD may protect the GI tract by altering the metabolism of bile acids (BAs)...
2017: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/28421401/liquid-marbles-as-bioreactors-for-the-study-of-three-dimensional-cell-interactions
#5
Raja K Vadivelu, Harshad Kamble, Ahmed Munaz, Nam-Trung Nguyen
Liquid marble as a bioreactor platform for cell-based studies has received significant attention, especially for developing 3D cell-based assays. This platform is particularly suitable for 3D in-vitro modeling of cell-cell interactions. For the first time, we demonstrated the interaction of olfactory ensheathing cells (OECs) with nerve debris and meningeal fibroblast using liquid marbles. As the transplantation of OECs can be used for repairing nerve injury, degenerating cell debris within the transplantation site can adversely affect the survival of transplanted OECs...
June 2017: Biomedical Microdevices
https://www.readbyqxmd.com/read/28419272/insecticidal-and-behavioral-avoidance-responses-of-anopheles-minimus-and-culex-quinquefasciatus-diptera-culicidae-to-three-synthetic-repellents
#6
Wasana Boonyuan, Sunaiyana Sathantriphop, Krajana Tainchum, Vithee Muenworn, Atchariya Prabaripai, Michael J Bangs, Theeraphap Chareonviriyaphap
Escape responses, knockdown (KD), and toxicity of laboratory strains of Anopheles minimus Theobald and Culex quinquefasciatus Say to three synthetic mosquito repellents, DEET (N, N-diethyl-3-methylbenzamide), IR3535, or picaridin, at 5% v/v concentrations, were evaluated using repellent-treated papers in standard WHO tube assays and an excito-repellency (ER) test chamber system. The tube assays recorded knockdown effects of each repellent immediately after 30-min exposure and the final morality following a 24-h holding period...
April 15, 2017: Journal of Medical Entomology
https://www.readbyqxmd.com/read/28418637/development-and-application-of-an-msall-based-approach-for-the-quantitative-analysis-of-linear-polyethylene-glycols-in-rat-plasma-by-liquid-chromatography-triple-quadrupole-time-of-flight-mass-spectrometry
#7
Xiaotong Zhou, Xiangjun Meng, Longmei Cheng, Chong Su, Yantong Sun, Lingxia Sun, Zhaohui Tang, John Paul Fawcett, Yan Yang, Jingkai Gu
Polyethylene glycols (PEGs) are synthetic polymers composed of repeating ethylene oxide subunits. They display excellent biocompatibility and are widely used as pharmaceutical excipients. To fully understand the biological fate of PEGs requires accurate and sensitive analytical methods for their quantitation. Application of conventional liquid chromatography-tandem mass spectrometry (LC-MS/MS) is difficult because PEGs have polydisperse molecular weights (MWs) and tend to produce multicharged ions in-source resulting in innumerable precursor ions...
April 18, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28417966/the-ecdysis-triggering-hormone-system-is-essential-for-successful-moulting-of-a-major-hemimetabolous-pest-insect-schistocerca-gregaria
#8
Cynthia Lenaerts, Dorien Cools, Rik Verdonck, Lina Verbakel, Jozef Vanden Broeck, Elisabeth Marchal
Insects are enclosed in a rigid exoskeleton, providing protection from desiccation and mechanical injury. To allow growth, this armour needs to be replaced regularly in a process called moulting. Moulting entails the production of a new exoskeleton and shedding of the old one and is induced by a pulse in ecdysteroids, which activates a peptide-mediated signalling cascade. In Holometabola, ecdysis triggering hormone (ETH) is the key factor in this cascade. Very little functional information is available in Hemimetabola, which display a different kind of development characterized by gradual changes...
April 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28417589/glutathione-stabilized-cu-nanocluster-based-fluorescent-probe-for-sensitive-and-selective-detection-of-hg-2-in-water
#9
Tingting Luo, Shiting Zhang, Yujue Wang, Meina Wang, Mei Liao, Xingming Kou
In this paper, an innovative and facile one-pot method for synthesizing water-soluble and stable fluorescent Cu nanoclusters (CuNCs), in which glutathione (GSH) served as protecting ligand and ascorbic acid (AA) as reducing agent was reported. The resultant CuNCs emitted blue-green fluorescence at 440 nm, with a quantum yield (QD) of about 3.08%. In addition, the prepared CuNCs exhibited excellent properties such as good water solubility, photostability and high stability toward high ionic strength. On the basis of the selective quenching of Hg(2+) on CuNCs fluorescence, which may be the result of Hg(2+) ion-induced aggregation of the CuNCs, the CuNCs was used for the selective and sensitive determination of Hg(2+) in aqueous solution...
April 18, 2017: Luminescence: the Journal of Biological and Chemical Luminescence
https://www.readbyqxmd.com/read/28417367/measuring-the-biomechanical-loosening-action-of-bacterial-expansins-on-paper-and-plant-cell-walls
#10
Daniel J Cosgrove, Nathan K Hepler, Edward R Wagner, Daniel M Durachko
Expansins are proteins that loosen plant cell walls but lack enzymatic activity. Here, we describe two protocols tailored to measure the biomechanical activity of bacterial expansin. The first assay relies on weakening of filter paper by expansin. The second assay is based on induction of creep (long-term, irreversible extension) of plant cell wall samples.
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28415435/developing-an-electrochemical-sensor-based-on-a-carbon-paste-electrode-modified-with-nano-composite-of-reduced-graphene-oxide-and-cufe2o4-nanoparticles-for-determination-of-hydrogen-peroxide
#11
Ali Benvidi, Mohammad Taghi Nafar, Shahriar Jahanbani, Marzieh Dehghan Tezerjani, Masoud Rezaeinasab, Sudabeh Dalirnasab
In this paper, a highly sensitive voltammetric sensor based on a carbon paste electrode with CuFe2O4 nanoparticle (RGO/CuFe2O4/CPE) was designed for determination of hydrogen peroxide (H2O2). The electrocatalytic reduction of H2O2 was examined using various techniques such as cyclic voltammetry (CV), chronoamperometry, amperometry and differential pulse voltammetry (DPV). CuFe2O4 nanoparticles were synthesized by co-precipitation method and characterized with scanning electron microscopy (SEM), Transmission electron microscopy (TEM), X-ray diffraction (XRD), and Fourier transform infrared (FTIR) techniques...
June 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28411630/an-immunochromatographic-assay-for-carcinoembryonic-antigen-on-cotton-thread-using-a-composite-of-carbon-nanotubes-and-gold-nanoparticles-as-reporters
#12
Xiaobo Jia, Tingting Song, Yan Liu, Lili Meng, Xun Mao
This paper describes a low-cost, sensitive, visual and rapid immunochromatographic assay method on cotton thread for carcinoembryonic antigen (CEA) detection by using novel carbon nanotube/gold nanoparticles (CNT/GNPs) nanocomposite reporter probe. CEA, a lung cancer protein biomarker, was used as analyte to demonstrate the principle of the immunochromatographic assay on cotton thread biosensor. In the presence of target CEA, the decreasing aggregation amount of CNT/GNPs nanocomposite reporter probes on the test zone induced directly readout by naked eye...
May 29, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28407817/preliminary-study-of-the-ul55-gene-based-on-infectious-chinese-virulent-duck-enteritis-virus-bacterial-artificial-chromosome-clone
#13
Ying Wu, Yangguang Li, Mingshu Wang, Kunfeng Sun, Renyong Jia, Shun Chen, Dekang Zhu, Mafeng Liu, Qiao Yang, Xinxin Zhao, Xiaoyue Chen, Anchun Cheng
BACKGROUND: Lethal Duck Enteritis Virus (DEV) infection can cause high morbidity and mortality of many species of waterfowl within the order Anseriformes. However, little is known about the function of viral genes including the conserved UL55 gene among alpha herpes virus due to the obstacles in maintenance and manipulation of DEV genome in host cells. METHODS: In this paper, we constructed an infectious bacteria artificial chromosome (BAC) clone of the lethal clinical isolate duck enteritis virus Chinese virulent strain (DEV CHv) by inserting a transfer vector containing BAC mini-F sequence and selection marker EGFP into UL23 gene using homologous recombination...
April 13, 2017: Virology Journal
https://www.readbyqxmd.com/read/28407808/nested-pcr-assay-for-detection-of-schistosoma-japonicum-infection-in-domestic-animals
#14
Xin Zhang, Chuan-Chuan He, Jin-Ming Liu, Hao Li, Ke Lu, Zhi-Qiang Fu, Chuan-Gang Zhu, Yi-Ping Liu, Lai-Bao Tong, De-Bao Zhou, Li Zha, Yang Hong, Ya-Mei Jin, Jiao-Jiao Lin
BACKGROUND: Schistosomiasis japonica is a common zoonosis. Domestic animals are the primary source of infection and play an important role in disease transmission. The prevalence and infectivity of this disease in domestic animals in China have significantly decreased and, for this reason, diagnostics with a higher sensitivity have become increasingly necessary. It was reported that polymerase chain reaction (PCR)-based methods could be used to detect schistosome infection in humans and animals and presented a high sensitivity and specificity...
April 13, 2017: Infectious Diseases of Poverty
https://www.readbyqxmd.com/read/28406607/beyond-wicking-expanding-the-role-of-patterned-paper-as-the-foundation-for-an-analytical-platform
#15
Syrena C Fernandes, Jenna A Walz, Daniel J Wilson, Jessica C Brooks, Charles R Mace
This Feature article discusses the use of patterned paper as a platform for the development of low-cost microfluidic devices and analytical tests that are designed for use in limited-resource settings. While a number of assays for soluble analytes have been developed using paper-based microfluidic devices, the detection and analysis of blood cells has remained an outstanding challenge. Expanding the utility of paper to determine cell counts would address a critical disparity that exists in managing healthcare and diagnosing diseases at the point-of-care...
April 13, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28402653/host-cell-protein-profiling-by-targeted-and-untargeted-analysis-of-data-independent-acquisition-mass-spectrometry-data-with-parallel-reaction-monitoring-verification
#16
Simion Kreimer, Yuanwei Gao, Somak Ray, Mi Jin, Zhijun Tan, Nesredin A Mussa, Li Tao, Zhengjian Li, Alexander R Ivanov, Barry L Karger
Host cell proteins (HCPs) are process-related impurities of biopharmaceuticals that remain at trace levels despite multiple stages of downstream purification. Currently, there is interest in implementing LC-MS in biopharmaceutical HCP profiling alongside conventional ELISA, because individual species can be identified and quantitated. Conventional data-dependent LC-MS is hampered by the low concentration of HCP-derived peptides, which are 5-6 orders of magnitude less abundant than the biopharmaceutical-derived peptides...
April 12, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28401754/improving-sample-distribution-homogeneity-in-three-dimensional-microfluidic-paper-based-analytical-devices-by-rational-device-design
#17
Giorgio Gianini Morbioli, Thiago Mazzu-Nascimento, Luis Aparecido Milan, Amanda M Stockton, Emanuel Carrilho
Paper-based devices are a portable, user-friendly, and affordable technology that is one of the best analytical tools for inexpensive diagnostic devices. Three-dimensional microfluidic paper-based analytical devices (3D-μPADs) are an evolution of single layer devices and they permit effective sample dispersion, individual layer treatment, and multiplex analytical assays. Here, we present the rational design of a wax-printed 3D-μPAD that enables more homogeneous permeation of fluids along the cellulose matrix than other existing designs in the literature...
April 19, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28400066/development-of-a-fast-workflow-to-screen-the-charge-variants-of-therapeutic-antibodies
#18
Elsa Wagner-Rousset, Szabolcs Fekete, Laura Morel-Chevillet, Olivier Colas, Nathalie Corvaïa, Sarah Cianférani, Davy Guillarme, Alain Beck
Chemical or enzymatic modifications of therapeutic monoclonal antibodies (mAbs) having high risk towards safety and efficacy are defined as critical quality attributes (CQAs). During therapeutic mAbs process development, a variety of analytical techniques have to be used for the thorough characterization and quantitative monitoring of CQAs. This paper describes the development of a rapid analytical platform to assess and rank charge variants of mAbs. The workflow is first based on a cation exchange chromatography (CEX) comparative analysis of intact IgGs versus F(ab)'2 and Fc sub-domains generated by IdeS digestion...
February 27, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/28397548/pre-natal-diagnosis-of-thalassaemia-in-sri-lanka-a-ten-year-review
#19
Kalinga Khemal Nanayakkara, Undugodage Ganganath Rodrigo, Kuda Liyanage Nandika Perera, Chinthani Deepthi Nanayakkara
Thalassaemia is the commonest monogenic disease in Sri Lanka, affecting over 3500 children and half-a-million thalassaemia carriers. This is a review of 82 amniocenteses performed from 2006 to 2016, in the largest prenatal diagnoses study for thalassaemia carried out in Sri Lanka. Amniocenteses were performed between 11 and 12 weeks of ultrasonically confirmed gestation, on mothers with previous thalassaemia major children pregnant for the second time and nulliparous thalassaemia trait women married to trait partners...
April 11, 2017: Journal of Obstetrics and Gynaecology: the Journal of the Institute of Obstetrics and Gynaecology
https://www.readbyqxmd.com/read/28395246/electrochemical-and-optical-study-of-metallothionein-interactions-with-prion-proteins
#20
Alzbeta Cardova, Pavlina Adam, Stefano Mariani, Lukas Richtera, Zbynek Heger, Jan Labuda, Maria Minunni, Vojtech Adam
The prion protein (PrP(C)) can be structurally shifted to its PrP(Sc) isoform causing a wide range of neurodegenerative diseases, which are currently incurable. There is an evidence that metallothioneins (MTs), and especially MT-3, are associated with neurodegenerative diseases. PrP(C) and MTs play pivotal roles in maintaining metal homeostasis; therefore, it is conceivable that each of them has its own significance in prion diseases. In this paper, we study the nature of interactions between PrP(C), MT, and copper ions, Cu(II), using the method of differential pulse voltammetry (DPV) coupled with adsorptive transfer stripping technique (AdTS)...
March 23, 2017: Journal of Pharmaceutical and Biomedical Analysis
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