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https://www.readbyqxmd.com/read/28239509/system-design-and-development-of-a-robotic-device-for-automated-venipuncture-and-diagnostic-blood-cell-analysis
#1
Max L Balter, Alvin I Chen, Alex Fromholtz, Alex Gorshkov, Tim J Maguire, Martin L Yarmush
Diagnostic blood testing is the most prevalent medical procedure performed in the world and forms the cornerstone of modern health care delivery. Yet blood tests are still predominantly carried out in centralized labs using large-volume samples acquired by manual venipuncture, and no end-to-end solution from blood draw to sample analysis exists today. Our group is developing a platform device that merges robotic phlebotomy with automated diagnostics to rapidly deliver patient information at the site of the blood draw...
October 2016: Proceedings of the ... IEEE/RSJ International Conference on Intelligent Robots and Systems
https://www.readbyqxmd.com/read/28238809/in-situ-protein-determination-to-monitor-contamination-in-a-centrifugal-partition-chromatograph
#2
Feriel Bouiche, Karine Faure
Centrifugal partition chromatography (CPC) works with biphasic liquid systems including aqueous two-phase systems. Metallic rotors are able to retain an aqueous stationary phase able to purify proteins. But the adhesion of proteins to solid surface may pose a cross-contamination risk during downstream processes. So it is of utmost importance to ensure the cleanliness of the equipment and detect possible protein contamination in a timely manner. Thereby, a direct method that allows the determination of the effective presence of proteins and the extent of contamination in the metallic CPC rotors was developed...
February 23, 2017: Analytical Biochemistry
https://www.readbyqxmd.com/read/28237255/high-affinity-truncated-dna-aptamers-for-the-development-of-fluorescence-based-progesterone-biosensors
#3
Hani Alhadrami, Raja Chinnappan, Shimaa Eissa, Anas Abdel Rahamn, Mohammed Zourob
Aptamers have shown a number of potential applications in sensing and therapeutic due to the high affinity and specificity towards their target molecules. Not all the nucleotides in the full length aptamers are involved in the binding with their targets. The non-binding domain of the aptamer may affect the binding affinity of the aptamer-target complex. Mapping the aptamer binding region could increase the affinity and the specificity. In this paper, we designed aptamer-based fluorescence sensors from a truncated progesterone (P4) aptamer...
February 22, 2017: Analytical Biochemistry
https://www.readbyqxmd.com/read/28227780/impact-of-prostate-biopsy-tumor-amount-on-imaging-based-prognostics-employing-transductive-semi-supervised-regression
#4
Faisal M Khan, Casimir A Kulikowski, Faisal M Khan, Casimir A Kulikowski, Casimir A Kulikowski, Faisal M Khan
For newly diagnosed prostate cancer patients with a positive biopsy, there are a variety of treatment options to consider. To aid physicians and patients in their decision making, a variety of predictive assays have emerged within the last decade, many of them imaging based. These assays build predictive models for survival analysis to provide personalized risk assessments for the patients. However, there have rarely been any published studies on how the amount of tumor in the positive prostate biopsy affects the predictive power of these imaging based assays...
August 2016: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/28225595/colorimetric-and-electrochemical-bacteria-detection-using-printed-paper-and-transparency-based-analytic-devices
#5
Jaclyn A Adkins, Katherine Boehle, Colin Friend, Briana Chamberlain, Bledar Bisha, Charles S Henry
The development of transparency-based electrochemical and paper-based colorimetric analytic detection platforms is presented as complimentary methods for food and waterborne bacteria detection from a single assay. Escherichia coli and Enterococcus species, both indicators of fecal contamination, were detected using substrates specific to enzymes produced by each species. β-galactosidase (β-gal) and β-glucosidase (β-gluco) are both produced by E. coli, while β-glucosidase (β-gluco) is produced by Enterococcus spp...
February 22, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28225117/gold-nanoparticles-and-the-corresponding-filter-membrane-as-chemosensors-and-adsorbents-for-dual-signal-amplification-detection-and-fast-removal-of-mercury-ii
#6
Gaosong Chen, Jun Hai, Hao Wang, Weisheng Liu, Fengjuan Chen, Baodui Wang
Nowadays, the development of a multifunction system for the simultaneous multiple signal amplification detection and fast removal of Hg(2+) remains a major challenge. Herein, we for the first time used gold nanoparticles (Au NPs) and the corresponding filter membrane as chemosensors and adsorbents for dual signal amplification detection and fast removal of Hg(2+). Such a system was based on the formation of gold amalgam and a gold amalgam-based reaction between rhodamine B (RhB) and NaBH4 with fluorescence and colorimetric sensing functions...
February 22, 2017: Nanoscale
https://www.readbyqxmd.com/read/28223418/papers-of-note-in-science-translational-medicine9-377
#7
Leslie K Ferrarelli
This week's articles report an integrated cell-based assay to test cardiac toxicity of anticancer drugs and discover a pathogen recognized by autoimmune antibodies involved in a form of epilepsy.
February 21, 2017: Science Signaling
https://www.readbyqxmd.com/read/28222780/advanced-biosensors-for-detection-of-pathogens-related-to-livestock-and-poultry
#8
REVIEW
Jasmina Vidic, Marisa Manzano, Chung-Ming Chang, Nicole Jaffrezic-Renault
Infectious animal diseases caused by pathogenic microorganisms such as bacteria and viruses threaten the health and well-being of wildlife, livestock, and human populations, limit productivity and increase significantly economic losses to each sector. The pathogen detection is an important step for the diagnostics, successful treatment of animal infection diseases and control management in farms and field conditions. Current techniques employed to diagnose pathogens in livestock and poultry include classical plate-based methods and conventional biochemical methods as enzyme-linked immunosorbent assays (ELISA)...
February 21, 2017: Veterinary Research
https://www.readbyqxmd.com/read/28218726/biomarkers-in-pediatric-community-acquired-pneumonia
#9
REVIEW
Nicola Principi, Susanna Esposito
Community-acquired pneumonia (CAP) is an infectious disease caused by bacteria, viruses, or a combination of these infectious agents. The severity of the clinical manifestations of CAP varies significantly. Consequently, both the differentiation of viral from bacterial CAP cases and the accurate assessment and prediction of disease severity are critical for effectively managing individuals with CAP. To solve questionable cases, several biomarkers indicating the etiology and severity of CAP have been studied...
February 19, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28214915/evaluation-of-the-gard-assay-in-a-blind-cosmetics-europe-study
#10
Henrik Johansson, Robin Gradin, Andy Forreryd, Maria Agemark, Kathrin Zeller, Angelica Johansson, Olivia Larne, Erwin van Vliet, Carl Borrebaeck, Malin Lindstedt
Chemical hypersensitivity is an immunological response towards foreign substances, commonly referred to as sensitizers, which gives rise primarily to the clinical symptoms known as allergic contact dermatitis. For the purpose of mitigating risks associated with consumer products, chemicals are screened for sensitizing effects. Historically, such predictive screenings have been performed using animal models. However, due to industrial and regulatory demand, animal models for the purpose of sensitization assessment are being replaced by animal-free testing methods, a global trend that is spreading across industries and market segments...
February 17, 2017: ALTEX
https://www.readbyqxmd.com/read/28212001/rapid-method-for-the-quantification-of-reduced-and-oxidized-glutathione-in-human-plasma-and-saliva
#11
Kamonwad Ngamchuea, Christopher Batchelor-McAuley, Richard G Compton
A new method is developed to determine the concentrations of reduced (GSH) and oxidized glutathione (GSSG), and it enables the calculation of the GSH:GSSG ratios in human plasma and saliva samples. The assay is based on the masking of GSH in a GSH and GSSG mixture via a 1,4-addition reaction with p-benzoquinone (BQ), followed by enzymatic kinetic measurement. The enzyme, glutathione reductase, is highly specific to glutathione. Excess BQ can thus be easily removed by the addition of non-GSH thiols. The assay takes less than 2 min, is suitable for a short-time-scale study, and minimizes the in vitro underestimation of the GSH:GSSG ratio arising from the degradation of GSH and formation of GSSG...
February 17, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28207238/real-time-monitoring-of-post-surgical-and-post-traumatic-eye-injuries-using-multi-layered-electrical-biosensor-chip
#12
Muhammad Shuja Khan, Santosh K Misra, Aaron Schwartz-Duval, Enrique A Daza, Fatemeh Ostadhossein, Matthew Bowman, Akshay Jain, Gina Taylor, Deana McDonagh, Leanne T Labriola, Dipanjan Pan
Lack of current techniques for the early monitoring of bleb leaks and other post-traumatic or post-surgical ocular injury has posed an unmet clinical need for the development of new techniques. Present evaluation techniques use either subjective or non-quantitative approaches. At present, there are no FDA approved ocular devices that can directly measure ascorbic acid (AA) concentration levels for both tear film (TF) and aqueous humor (AH) at point-of-care (POC) level. Toward this aim, we present a novel point-of-care (POC) quantitative assay, called the Ocular Biosensor device, which can be used to evaluate the integrity of the anterior surface of the eye by measuring the concentration of AA in TF and AH...
February 16, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28197602/automating-multi-step-paper-based-assays-using-integrated-layering-of-reagents
#13
Sana Jahanshahi-Anbuhi, Balamurali Kannan, Kevin Pennings, M Monsur Ali, Vincent Leung, Karen Giang, Jingyun Wang, Dawn White, Yingfu Li, Robert H Pelton, John D Brennan, Carlos D M Filipe
We describe a versatile and simple method to perform sequential reactions on paper analytical devices by stacking dry pullulan films on paper, where each film contains one or more reagents or acts as a delay layer. Exposing the films to an aqueous solution of the analyte leads to sequential dissolution of the films in a temporally controlled manner followed by diffusive mixing of the reagents, so that sequential reactions can be performed. The films can be easily arranged for lateral flow assays or for spot tests (reactions take place sequentially in the z-direction)...
February 15, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28194457/disposable-cartridge-platform-for-rapid-detection-of-viral-hemorrhagic-fever-viruses
#14
Steven M Scherr, David S Freedman, Krystle N Agans, Alexandru Rosca, Erik Carter, Melody Kuroda, Helen E Fawcett, Chad E Mire, Thomas W Geisbert, M Selim Ünlü, John H Connor
Light microscopy is a straightforward and highly portable imaging approach that is used for the detection of parasites, fungi, and bacteria. The detection of individual virus particles has historically not been possible through this approach. Thus, characterization of virus particles is typically performed using high-energy approaches such as electron microscopy. These approaches require purification of virions away from its normal milieu, significant levels of expertise, and only count a small number of particles at a time...
February 14, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28194453/a-paper-based-platform-for-detection-of-viral-rna
#15
Daohong Zhang, David Broyles, Eric A Hunt, Emre Dikici, Sylvia Daunert, Sapna K Deo
Viral detection presents a host of challenges for even the most sensitive analytical techniques, and the complexity of common detection platforms typically preclude portability. With these considerations in mind, we designed a paper microzone plate-based virus detection system for the detection of viral genetic material that can be performed with simple instruments. The sensing system can detect viral cDNA reverse-transcribed from total RNA extraction by utilizing a biotinylated capture probe and an Alexa Fluor® 647-labeled reporter probe...
February 14, 2017: Analyst
https://www.readbyqxmd.com/read/28193356/multivalent-foldamer-based-affinity-assay-for-selective-recognition-of-a%C3%AE-oligomers
#16
Gábor Olajos, Éva Bartus, Ildikó Schuster, Gergely Lautner, Róbert E Gyurcsányi, Titanilla Szögi, Lívia Fülöp, Tamás A Martinek
Mimicking the molecular recognition functionality of antibodies is a great challenge. Foldamers are attractive candidates because of their relatively small size and designable interaction surface. This paper describes a sandwich type enzyme-linked immunoassay with a tetravalent β-peptide foldamer helix array as capture element and enzyme labeled tracer antibodies. The assay was found to be selective to β-amyloid oligomeric species with surface features transiently present in ongoing aggregation. In optimized conditions, with special emphasis on the foldamer immobilization, a detection limit of 5 pM was achieved with a linear range of 10-500 pM...
April 1, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28188826/convenient-expression-purification-and-quantitative-liquid-chromatography-tandem-mass-spectrometry-based-analysis-of-tet2-5-methylcytosine-demethylase
#17
Mohit Jaiswal, Subhradeep Bhar, Harika Vemula, Swami Prakash, V K Chaithanya Ponnaluri, William G Gutheil, Mridul Mukherji
5-Methylcytosine within CpG islands in DNA plays a crucial role in epigenetic transcriptional regulation during metazoan development. Recently, it has been established that the Ten-Eleven Translocation (TET) family, Fe(II)- and 2-oxoglutarate (2OG/αKG)-dependent oxygenases initiate 5-methylcytosine demethylation by iterative oxidation reactions. Mutations in the TET2 gene are frequently detected in patients with myeloid malignancies. Here, we describe the cloning of untagged human TET2 demethylase using Gateway technology and its efficient expression in E...
February 8, 2017: Protein Expression and Purification
https://www.readbyqxmd.com/read/28182587/evolution-of-quality-assurance-for-clinical-immunohistochemistry-in-the-era-of-precision-medicine-part-2-immunohistochemistry-test-performance-characteristics
#18
Emina E Torlakovic, Carol C Cheung, Corrado D'Arrigo, Manfred Dietel, Glenn D Francis, C Blake Gilks, Jacqueline A Hall, Jason L Hornick, Merdol Ibrahim, Antonio Marchetti, Keith Miller, J Han van Krieken, Soren Nielsen, Paul E Swanson, Mogens Vyberg, Xiaoge Zhou, Clive R Taylor
All laboratory tests have test performance characteristics (TPCs), whether or not they are explicitly known to the laboratorian or the pathologist. TPCs are thus also an integral characteristic of immunohistochemistry (IHC) tests and other in situ, cell-based molecular assays such as DNA or RNA in situ hybridization or aptamer-based testing. Because of their descriptive, in situ, cell-based nature, IHC tests have a limited repertoire of appropriate TPCs. Although only a few TPCs are relevant to IHC, proper selection of informative TPCs is nonetheless essential for the development of and adherence to appropriate quality assurance measures in the IHC laboratory...
February 2017: Applied Immunohistochemistry & Molecular Morphology: AIMM
https://www.readbyqxmd.com/read/28181281/tolerance-limits-and-tolerance-intervals-for-ratios-of-normal-random-variables-using-a-bootstrap-calibration
#19
Marilena Flouri, Shuyan Zhai, Thomas Mathew, Ionut Bebu
This paper addresses the problem of deriving one-sided tolerance limits and two-sided tolerance intervals for a ratio of two random variables that follow a bivariate normal distribution, or a lognormal/normal distribution. The methodology that is developed uses nonparametric tolerance limits based on a parametric bootstrap sample, coupled with a bootstrap calibration in order to improve accuracy. The methodology is also adopted for computing confidence limits for the median of the ratio random variable. Numerical results are reported to demonstrate the accuracy of the proposed approach...
February 9, 2017: Biometrical Journal. Biometrische Zeitschrift
https://www.readbyqxmd.com/read/28179996/a-quantitative-real-time-approach-for-discriminating-apoptosis-and-necrosis
#20
Asha Lekshmi, Shankara Narayanan Varadarajan, Santhik Subhasingh Lupitha, Deepa Indira, Krupa Ann Mathew, Aneesh Chandrasekharan Nair, Mydhily Nair, Tilak Prasad, Hari Sekar, Anurup Kochucherukkan Gopalakrishnan, Abitha Murali, Thankayyan Retnabai Santhoshkumar
Apoptosis and necrosis are the two major forms of cell death mechanisms. Both forms of cell death are involved in several physiological and pathological conditions and also in the elimination of cancer cells following successful chemotherapy. Large number of cellular and biochemical assays have evolved to determine apoptosis or necrosis for qualitative and quantitative purposes. A closer analysis of the assays and their performance reveal the difficulty in using any of these methods as a confirmatory approach, owing to the secondary induction of necrosis in apoptotic cells...
2017: Cell Death Discovery
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