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https://www.readbyqxmd.com/read/28103450/paper-based-analytical-devices-for-clinical-diagnosis-recent-advances-in-the-fabrication-techniques-and-sensing-mechanisms
#1
Mazhar Sher, Rachel Zhuang, Utkan Demirci, Waseem Asghar
There is a significant interest in developing inexpensive portable biosensing platforms for various applications including disease diagnostics, environmental monitoring, food safety, and water testing at the point-of-care (POC) settings. Current diagnostic assays available in the developed world require sophisticated laboratory infrastructure and expensive reagents. Hence, they are not suitable for resource-constrained settings with limited financial resources, basic health infrastructure, and few trained technicians...
January 19, 2017: Expert Review of Molecular Diagnostics
https://www.readbyqxmd.com/read/28102570/simultaneous-determination-of-free-and-total-prostate-specific-antigen-by-a-magnetic-particle-based-time-resolved-fluoroimmunoassay
#2
Bin Zhou, Jue Zhang, Zhongwei Lv, Jun Fan, Yi Zhang, Biao Huang
OBJECTIVES: In this paper, a novel, sensitive and rapid method to simultaneously determine the free and total prostate-specific antigen (fPSA and tPSA) in serum by combining a time-resolved fluoroimmunoassay (TRFIA) and immunomagnetic separation was described. METHODS: The new approach uses magnetic particles as an immobilization matrix and means of separation, whereas the luminescent europium (Eu) and samarium (Sm) chelates are used as probes. The proposed method was evaluated via a single-step, sandwich-type TRFIA immunoassay of tPSA and fPSA as model analytes in serum...
January 19, 2017: Journal of Clinical Laboratory Analysis
https://www.readbyqxmd.com/read/28101426/asymmetric-split-h-shape-nanoantennas-for-molecular-sensing
#3
I G Mbomson, S Tabor, B Lahiri, G Sharp, S G McMeekin, R M De La Rue, N P Johnson
In this paper we report on a very sensitive biosensor based on gold asymmetric nanoantennas that are capable of enhancing the molecular resonances of C-H bonds. The nanoantennas are arranged as arrays of asymmetric-split H-shape (ASH) structures, tuned to produce plasmonic resonances with reflectance double peaks within the mid-infrared vibrational resonances of C-H bonds for the assay of deposited films of the molecule 17β-estradiol (E2), used as an analyte. Measurements and numerical simulations of the reflectance spectra have enabled an estimated enhancement factor on the order of 10(5) to be obtained for a thin film of E2 on the ASH array...
January 1, 2017: Biomedical Optics Express
https://www.readbyqxmd.com/read/28099838/osteoarthritis-year-in-review-2016-biomarkers-biochemical-markers
#4
REVIEW
A Mobasheri, A-C Bay-Jensen, W E van Spil, J Larkin, M C Levesque
PURPOSE: The aim of this "Year in Review" article is to summarize and discuss the implications of biochemical marker related articles published between the Osteoarthritis Research Society International (OARSI) 2015 Congress in Seattle and the OARSI 2016 Congress in Amsterdam. METHODS: The PubMed/MEDLINE bibliographic database was searched using the combined keywords: 'biomarker' and 'osteoarthritis'. The PubMed/MEDLINE literature search was conducted using the Advanced Search Builder function (http://www...
January 2, 2017: Osteoarthritis and Cartilage
https://www.readbyqxmd.com/read/28095177/integrated-colony-imaging-analysis-and-selection-device-for-regenerative-medicine
#5
Edward Kwee, Edward E Herderick, Thomas Adams, James Dunn, Robert Germanowski, Frank Krakosh, Cynthia Boehm, James Monnich, Kimerly Powell, George Muschler
Stem and progenitor cells derived from human tissues are being developed as cell sources for cell-based assays and therapies. However, tissue-derived stem and progenitor cells are heterogeneous. Differences in observed clones of stem cells likely reflect important aspects of the underlying state of the source cells, as well as future potency for cell therapies. This paper describes a colony analysis and picking device that provides quantitative analysis of heterogeneous cell populations and precise tools for cell picking for research or biomanufacturing applications...
November 1, 2016: Journal of Laboratory Automation
https://www.readbyqxmd.com/read/28094839/improved-fluorescence-assays-to-measure-the-defects-associated-with-f508del-cftr-allow-identification-of-new-active-compounds
#6
Emily Langron, Michela I Simone, Clémence M S Delalande, Jean-Louis Reymond, David L Selwood, Paola Vergani
BACKGROUND AND PURPOSE: Cystic fibrosis (CF) is a debilitating disease caused by mutations in the cystic fibrosis transmembrane conductance regulator (CFTR) gene, which codes for a Cl-/HCO3 - channel. F508del, the most common CF-associated mutation, causes both gating and biogenesis defects in the CFTR protein. This paper describes the optimisation of two fluorescence assays, capable of measuring CFTR function and cellular localisation, and their use in a pilot drug screen. EXPERIMENTAL APPROACH: HEK293 cells expressing YFP-F508del-CFTR, in which halide sensitive YFP is tagged to the N-terminal of CFTR, were used to screen a small library of compounds based on the VX-770 scaffold...
January 17, 2017: British Journal of Pharmacology
https://www.readbyqxmd.com/read/28092954/modeling-and-simulation-for-toxicity-assessment
#7
Cristina Anton, Jian Deng, Yau Shu Wong, Yile Zhang, Weiping Zhang, Stephan Gabos, Dorothy Yu Huang, Can Jin
The effect of various toxicants on growth/death and morphology of human cells is investigated using the xCELLigence Real-Time Cell Analysis High Troughput in vitro assay. The cell index is measured as a proxy for the number of cells, and for each test substance in each cell line, time-dependent concentration response curves (TCRCs) are generated. In this paper we propose a mathematical model to study the effect of toxicants with various initial concentrations on the cell index. This model is based on the logistic equation and linear kinetics...
June 1, 2017: Mathematical Biosciences and Engineering: MBE
https://www.readbyqxmd.com/read/28074962/crossed-flow-microfluidics-for-high-throughput-screening-of-bioactive-chemical-cell-interactions
#8
Ziqiu Tong, Angela Ivask, Keying Guo, Scott McCormick, Enzo Lombi, Craig Priest, Nicolas H Voelcker
This paper describes the use of crossed laminar flow microfluidics for the selective capture of multiple cell types on-chip aiming for high throughput screening of various cell treatment compounds. Parallel laminar streams containing different cell types were perfused and captured on a cell adhesion protein-functionalized reaction area. Thereafter, parallel streams containing cell treatment solutions were delivered orthogonally over the captured cells. Multiple cell types and a range of cell treatment conditions could therefore be assessed in a single experiment...
January 11, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28073118/in-vitro-metabolism-of-artepillin-c-by-rat-and-human-liver-microsomes
#9
Daniel Blascke Carrão, Nayara Cristina Perez de Albuquerque, Lucas Maciel Mauriz Marques, Antônio Eduardo Miller Crotti, Alan Cesar Pilon, Vanderlan Da Silva Bolzani, Andresa Aparecida Berretta, Anderson Rodrigo Moraes de Oliveira
Artepillin C, a natural product present in the Brazilian green propolis, has several biological properties. Among these properties, the antitumor action of this product is noteworthy and makes it a promising drug candidate for the treatment of several types of cancer. This paper describes the in vitro metabolism of Artepillin C in rat and human liver microsomes. The rat model suggested a sigmoidal profile for the metabolism, adapted to the Hill's kinetic model. The enzymatic kinetic parameters were as follows: maximal velocity = 0...
January 10, 2017: Planta Medica
https://www.readbyqxmd.com/read/28061542/an-efficient-method-for-native-protein-purification-in-the-selected-range-from-prostate-cancer-tissue-digests
#10
Rumana Ahmad, Carrie D Nicora, Anil K Shukla, Richard D Smith, Wei-Jun Qian, Alvin Y Liu
BACKGROUND: Prostate cancer (CP) cells differ from their normal counterpart in gene expression. Genes encoding secreted or extracellular proteins with increased expression in CP may serve as potential biomarkers. For their detection and quantification, assays based on monoclonal antibodies are best suited for development in the clinical setting. One approach to obtain antibodies is to use recombinant proteins as immunogen. However, the synthesis of recombinant protein for each identified candidate is time-consuming and expensive...
December 2016: Chinese Clinical Oncology
https://www.readbyqxmd.com/read/28056740/novel-colchicine-derivatives-and-their-anti-cancer-activity
#11
Lorelei Johnson, Ing Swie Goping, Aja Rieger, Jonathan Y Mane, Torin Huzil, Asok Banerjee, Richard Luduena, Bashar Hassani, Philip Winter, Jack A Tuszynski
In this paper we provide an overview of the status of various colchicine derivatives in pre-clinical development with special focus on their anti-cancer activity. We discuss several groups of compounds that have been designed to differentially bind with specific affinities for tubulin β isotypes, especially in regard to βIII, which is commonly over-expressed in cancer. Computational prediction, protein-based and cell-based assays are summarized as well as some animal tests conducted on these compounds. It is concluded that an untapped potential exists for exploiting the colchicine scaffold as a pharmacophore with the possibility of increasing its affinity for tubulin isotypes over-expressed in cancer and decreasing it for normal cells thereby widening the therapeutic window...
January 4, 2017: Current Topics in Medicinal Chemistry
https://www.readbyqxmd.com/read/28054006/western-blot-data-using-two-distinct-anti-o-glcnac-monoclonal-antibodies-showing-unique-glycosylation-status-on-cellular-proteins-under-2-deoxy-d-glucose-treatment
#12
Tetsuya Okuda
Protein modification by O-linked N-acetylglucosamine (O-GlcNAcylation) is one of the post transcriptional modifications occurring on cellular proteins. This paper provides a data set relating to the O-GlcNAcylation of cellular proteins detected by RL2 and CTD110.6 antibodies, which are commonly used for detection of protein O-GlcNAcylation, in 2-deoxy-d-glucose (2DG)-treated human teratocarcinoma NCCIT cells in support of the research article entitled "A novel, promoter-based, target-specific assay identifies 2-deoxy-d-glucose as an inhibitor of globotriaosylceramide biosynthesis" (Okuda et al...
February 2017: Data in Brief
https://www.readbyqxmd.com/read/28050984/evaluation-of-bacteriological-profile-in-the-apical-root-segment-of-the-patients-with-primary-apical-periodontitis
#13
Aravind Tatikonda, N Sudheep, Krishna P Biswas, K Gowtham, Sudarshan Pujari, Padam Singh
INTRODUCTION: Apical periodontitis usually results from bacterial accumulation and contamination occurring in the root-canal system, and extending beyond the apical foramen to involve the periapical tissues. Literature has a paucity of the studies that stress on the division and analysis of the pulp canal segments. The reason for this disparity might be the technique used for collecting the samples from the pulp canals. Hence, we carried out the present study to evaluate the microbial flora in the apical part of the roots with necrotic pulp canals...
January 1, 2017: Journal of Contemporary Dental Practice
https://www.readbyqxmd.com/read/28050904/paper-based-analytical-biosensor-chip-designed-from-graphene-nanoplatelet-amphiphilic-di-block-co-polymer-composite-for-cortisol-detection-in-human-saliva
#14
Muhammad Khan, Santosh K Misra, Zhen Wang, Enrique A Daza, Aaron Schwartz-Duval, Joseph Kus, Debanjan Pan, Dipanjan Pan
Cortisol has been identified as a biomarker in saliva to monitor psychological stress. In this work, we report label-free paper-based electrical biosensor chip to quantify salivary cortisol at a point-of-care (POC) level. A high specificity of the sensor chip to detect cortisol with detection limit of 3 pg/mL was achieved by conjugating anti-cortisol antibody (anti-CAB) on top of gold (Au) microelectrodes using 3,3'-Dithiodipropionic acid di(N-hydroxysuccinimide ester (DTSP) - as self-assembled monolayer (SAM) agent...
January 4, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28043893/a-novel-microstructural-interpretation-for-the-biomechanics-of-mouse-skin-derived-from-multiscale-characterization
#15
Barbara Lynch, Stéphane Bancelin, Christelle Bonod-Bidaud, Jean-Baptiste Gueusquin, Florence Ruggiero, Marie-Claire Schanne-Klein, Jean-Marc Allain
: Skin is a complex, multi-layered organ, with important functions in the protection of the body. The dermis provides structural support to the epidermal barrier, and thus has attracted a large number of mechanical studies. As the dermis is made of a mixture of stiff fibres embedded in a soft non-fibrillar matrix, it is classically considered that its mechanical response is based on an initial alignment of the fibres, followed by the stretching of the aligned fibres. Using a recently developed setup combining multiphoton microscopy with mechanical assay, we imaged the fibres network evolution during dermis stretching...
December 30, 2016: Acta Biomaterialia
https://www.readbyqxmd.com/read/28040514/the-development-of-a-multiplex-real-time-rt-pcr-for-the-detection-of-adenovirus-astrovirus-rotavirus-and-sapovirus-from-stool-samples
#16
Susan Bennett, Rory N Gunson
Viral gastroenteritis is a major health problem with significant morbidity and economic consequences. Viral gastroenteritis is caused by a number of viruses, including norovirus, rotavirus, adenovirus, astrovirus, and sapovirus. Conventional diagnosis is based on direct antigen detection and electron microscopy, however enzyme immunoassay's are insensitive and not available for all relevant pathogens, and electron microscope (EM) is no longer routinely carried out in most laboratories. Most laboratories now offer norovirus real-time PCR testing however the availability of other assays is variable...
December 28, 2016: Journal of Virological Methods
https://www.readbyqxmd.com/read/28038327/synthesis-of-new-sarsasapogenin-derivatives-with-cytotoxicity-and-apoptosis-inducing-activities-in-human-breast-cancer-mcf-7%C3%A2-cells
#17
Wenbao Wang, Di Wang, Zedan Wang, Guodong Yao, Xue Li, Pinyi Gao, Lingzhi Li, Yan Zhang, Shaojie Wang, Shaojiang Song
Based on the fact that Timosaponin A-III, a saponin isolated from the rhizome of Anemarrhena asphodeloides, is a promising bioactive lead compound in the treatment of cancer, structural modification at the C3 and C26 positions of sarsasapogenin has always been the focus of our structure-activity investigations. In this paper, we describe the synthesis of a range of new derivatives 5a-5o and the evaluation of their antitumor activities in a panel of six human cancer cell lines using the MTT assay in vitro. The results obtained showed that compounds 5h, 5i, and 5n exhibited significant cytotoxic activities against the six cell lines, being more potent than their parent compound sarsasapogenin...
December 20, 2016: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/28034659/toxins-and-pharmacologically-active-compounds-from-species-of-the-family-bufonidae-amphibia-anura
#18
REVIEW
Candelario Rodríguez, Louise Rollins-Smith, Roberto Ibáñez, Armando A Durant-Archibold, Marcelino Gutiérrez
ETHNOPHARMACOLOGICAL RELEVANCE: Among amphibians, 15 of the 47 species reported to be used in traditional medicines belong to the family Bufonidae, which demonstrates their potential in pharmacological and natural products research. For example, Asian and American tribes use the skin and the parotoid gland secretions of some common toads in the treatment of hemorrhages, bites and stings from venomous animals, skin and stomach disorders, as well as several types of cancers. OVERARCHING OBJECTIVE: In addition to reviewing the occurrence of chemical constituents present in the family Bufonidae, the cytotoxic and biomedical potential of the active compounds produced by different taxa are presented...
December 26, 2016: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/28032464/discovery-of-macrocyclic-pyrimidines-as-mertk-specific-inhibitors
#19
Andrew McIver, Weihe Zhang, Qingyang Liu, Xinpeng Jiang, Michael Stashko, James Nichols, Michael Miley, Jacqueline Norris-Drouin, Mischa Machius, Deborah DeRyckere, Edgar Wood, Douglas Graham, H Shelton Earp, Dmitri Kireev, Stephen Frye, Xiaodong Wang
Macrocycles have attracted significant attention in drug discovery recently. In fact, a few de novo designed macrocyclic kinases inhibitors are currently in clinical trials with good potency and selectivity for their intended target. In this paper, we have successfully engaged a structure-based drug design approach to discover macrocyclic pyrimidines as potent Mer tyrosine kinase (MerTK)-specific inhibitors. An enzyme-linked immunosorbent assay (ELISA) in a 384-well format was employed to evaluate the inhibitory activity of macrocycles in a cell-based assay assessing tyrosine phosphorylation of MerTK...
December 28, 2016: ChemMedChem
https://www.readbyqxmd.com/read/28029031/inkjet-printing-enzyme-inhibitory-assay-based-on-determination-of-ejection-volume
#20
Jungmi Lee, Annie Agnes Suganya Samson, Joon Myong Song
An accurate, rapid, and cost-effective methodology for enzyme inhibitor assays is highly needed for large-scale screening to evaluate the efficacy of drugs at the molecular level. For the first time, we have developed an inkjet printing-based enzyme inhibition assay for the assessment of drug activity using a conventional inkjet printer composed of four cartridges. The methodology is based on the determination of the number of mole of the drug on the printed surface. The number of mole was quantified through the volume of substance ejected onto the printed surface...
December 27, 2016: Analytical Chemistry
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