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paper based assay

Chiara Ciccarese, Rodolfo Montironi, Michelangelo Fiorentino, Guido Martignoni, Matteo Brunelli, Roberto Iacovelli, Antonio Lopez-Beltran, Liang Cheng, Marina Scarpelli, Holger Moch, Giampaolo Tortora, Francesco Massari
Prostate cancer is a common malignancy with highly molecular heterogeneity responsible for a wide spectrum of clinical behavior. To date, several treatment options are available, whose selection is currently based mainly on clinical criteria. Given the weakness of conventional imaging and PSA assay, the identification of a prognostic and predictive biomarker for choosing the appropriate treatment and monitoring its efficacy is a very topical issue in prostate cancer management. Circulating tumor cells (CTCs) have substantial promise for early tumor diagnosis, disease recurrence and metastatic spread monitoring as well as for biological tumor characterization, thus representing a reliable translational real-time biomarkers of prostate cancer...
May 18, 2017: Current Drug Metabolism
Cui Liu, Dianhua Ning, Cheng Zhang, Zhengjie Liu, Ruilong Zhang, Jun Zhao, Tingting Zhao, Bianhua Liu, Zhongping Zhang
Classical pH test papers are widely employed to measure the acid-base degree of media in a qualitative and semi-quantitative way. However, the extension of the portable and inexpensive methods to a wide range of analytes so as to liberate us from the tediousness of instrumental assays remains unsuccessful. Here, we report a novel kind of dual-colored carbon dots (CDs) ratiometric-fluorescent test paper for the semi-quantitative assay of copper ions (Cu2+) by a dosage-sensitive color evolution. The creation of the test paper is based on the two interesting findings: on the one hand, the residual p-phenylenediamine (p-PDA) at the surface of as-synthesized red-emissive CDs (r-CDs) efficiently binds Cu2+ ions to produce a strong visible absorption that overlaps with the emission of blue CDs (b-CDs), on the other hand, Cu2+ ions render small b-CDs adsorbed onto the surface of larger-sized r-CDs through its dual-coordinating interactions with the surface ligands of both r-CDs and b-CDs...
May 18, 2017: ACS Applied Materials & Interfaces
Xudong Zhang, William Wang, Fang Li, Ioana Voiculescu
This paper presents the fabrication and testing of a novel stretchable electric cell-substrate impedance sensing (ECIS) lab on a chip device. This is the first time that ECIS electrodes were fabricated on a stretchable polydimethylsiloxane (PDMS) substrate and ECIS measurements were performed on mammalian cells exposed to cyclic strain. The stretchable ECIS biosensors simulate in vitro the dynamic environment of organisms, such as pulsation, bending and stretching, which enables investigations on cell behavior that undergoes mechanical stimuli in biological tissue...
May 17, 2017: Lab on a Chip
Myeong-Sub Song, Simranjeet Singh Sekhon, Woo-Ri Shin, Hyung Cheol Kim, Jiho Min, Ji-Young Ahn, Yang-Hoon Kim
In this paper, a Whole-Bacteria SELEX (WB-SELEX) strategy was adopted to isolate specific aptamers against Shigella sonnei. Real-time PCR amplification and post-SELEX experiment revealed that the selected aptmers possessed a high binding affinity and specificity for S. sonnei. Of the 21 aptamers tested, the C(t) values of the SS-3 and SS-4 aptamers (Ct = 13.89 and Ct = 12.23, respectively) had the lowest value compared to other aptamer candidates. The SS-3 and SS-4 aptamers also displayed a binding affinity (KD) of 39...
May 17, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
Haiyan Fu, Juanhua Yang, Lin Guo, Jinfang Nie, Qiaobo Yin, Lang Zhang, Yun Zhang
In this article, we describe a facile method named as Rubik's Cube stamping (RCS) for equipment-free fabrication of microfluidic paper-based analytical devices (μPADs). RCS is inspired by the worldwide ubiquitous RC toy and requires no specialized electric equipment other than a classical six-faced RC that is assembled with home-made small iron components. It can pattern various rosin microstructures in paper simply by either using different functional faces of the modified RC or applying its internal pivot mechanism to adjust the components' patterning forms on one functional face...
May 6, 2017: Biosensors & Bioelectronics
Caitlin E Anderson, Carly A Holstein, Eva-Maria Strauch, Steven Bennett, Aaron Chevalier, Jorgen W Nelson, Elain Fu, David Baker, Paul Yager
Influenza is a ubiquitous and recurring infection that results in approximately 500,000 deaths globally each year. Commercially available rapid diagnostic tests are based upon detection of the influenza nucleoprotein, which are limited in that they are unable to differentiate by species and require an additional viral lysis step. Sample pre-processing can be minimized or eliminated by targeting the intact influenza virus, thereby reducing assay complexity and leveraging the large number of hemagglutinin proteins on the surface of each virus...
May 12, 2017: Analytical Chemistry
Ali Hossein Rezayan, Negar Firoozi, Somayyeh Kheirjou, Seyed Jamal Tabatabaei Rezaei, Mohammad Reza Nabid
In this paper, the focus is on a new kind of biodegradable semi-interpenetrating polymer networks, which is derived from ɛ-caprolactone, lactide, 1,4-butane diisocyanate and ethylenediamine and also its potential has been investigated in soft tissue engineering applications. The polymers were characterized by nuclear magnetic resonance (NMR) spectrometry, Fourier transform infrared spectroscopy (FT-IR), differential thermal analysis (DTA), differential scanning calorimetry (DSC), and thermogravimetric analysis (TGA)...
2017: Iranian Journal of Pharmaceutical Research: IJPR
Muhamad Noor Alfarizal Kamarudin, Md Moklesur Rahman Sarker, Habsah Abdul Kadir, Long Chiau Ming
ETHNOPHARMACOLOGICAL RELEVANCE: Clinacanthus nutans (Burm. f.) Lindau, a widely used medicinal plant, is extensively grown in tropical Asia and Southeast Asian countries. C. nutans, with its broad spectrum of pharmacological activities, has been traditionally used to treat cancer, inflammatory disorders, diabetes, insect bites, and skin problems, consumed as a vegetable, mixed with fresh juices, in concoctions, and as a whole plant. The present review analyzes the advances in the ethnopharmacology, phytochemistry, pharmacology, and toxicology of C...
May 8, 2017: Journal of Ethnopharmacology
Florian Roeber, Ebrahim Bani Hassan, Philip Skuce, Alison Morrison, Edwin Claerebout, Stijn Casaert, David R Homer, Simon Firestone, Mark Stevenson, Lee Smith, John Larsen
Detecting the genera and species of gastrointestinal (GI) nematode infections in faecal samples obtained from cattle requires the incubation of faeces ('larval culture') followed by identification of the third-stage larvae that are harvested after 10-14days. Substantial research in the development of PCR-based methods for the rapid and specific identification GI nematodes has been conducted for small ruminants, whilst only few such assays have been developed for cattle. In the present paper we describe the development of an automated, robotic PCR platform for the detection and genus and/or species-specific identification of GI nematodes from bovine faecal samples...
May 30, 2017: Veterinary Parasitology
Younggeun Park, Byunghoon Ryu, Bo-Ram Oh, Yujing Song, Xiaogan Liang, Katsuo Kurabayashi
Monitoring of the time-varying immune status of a diseased host often requires rapid and sensitive detection of cytokines. Metallic nanoparticle-based localized surface plasmon resonance (LSPR) biosensors hold promise to meet this clinical need by permitting label-free detection of target biomolecules. These biosensors, however, continue to suffer from relatively low sensitivity as compared to conventional immunoassay methods that involve labeling processes. Their response speeds also need to be further improved to enable rapid cytokine quantification for critical care in a timely manner...
May 16, 2017: ACS Nano
Claudia Zani, Franco Bisceglie, Francesco Maria Restivo, Donatella Feretti, Marianna Pioli, Francesca Degola, Serena Montalbano, Serena Galati, Giorgio Pelosi, Gaia V C Viola, Mauro Carcelli, Dominga Rogolino, Elisabetta Ceretti, Annamaria Buschini
Aflatoxins represent a serious problem for a food economy based on cereal cultivations used to fodder animal and for human nutrition. The aims of our work are two-fold: first, to perform an evaluation of the activity of newly synthesized thiosemicarbazone compounds as antifungal and anti-mycotoxin agents and, second, to conduct studies on the toxic and genotoxic hazard potentials with a battery of tests with different endpoints. In this paper we report an initial study on two molecules: S-4-isopropenylcyclohexen-1-carbaldehydethiosemicarbazone and its metal complex, bis(S-4-isopropenylcyclohexen-1-carbaldehydethiosemicarbazonato)nickel (II)...
May 5, 2017: Food and Chemical Toxicology
Katherine E Boehle, Jake Gilliand, Christopher R Wheeldon, Amethyst Holder, Jaclyn A Adkins, Brian J Geiss, Elizabeth P Ryan, Charles S Henry
Antimicrobial resistance (AMR), the ability of a bacterial species to resist the action of an antimicrobial drug, has been on the rise due to the widespread use of antimicrobial agents. Per the World Health Organization, AMR has an estimated annual cost of USD 34 billion in the US and is predicted to be the number one cause of death worldwide by 2050. One way AMR bacteria can spread, and by which individuals can contract AMR infections, is through contaminated water. Monitoring AMR bacteria in the environment currently requires that samples be transported to a central laboratory for slow and labor intensive tests...
May 5, 2017: Angewandte Chemie
Bowei Li, Lijuan Yu, Ji Qi, Longwen Fu, Peiqing Zhang, Lingxin Chen
This paper describes a novel strategy for fabricating the movable valve on paper-based microfluidic devices to manipulate capillary-driven fluids. The movable valve fabrication is first realized using hollow rivets as the holding center to control the paper channel in different layer movement that results in the channel's connection or disconnection. The relatively simple valve fabrication procedure is robust, versatile, and compatible with microfluidic paper-based analytical devices (μPADs) with differing levels of complexity...
May 15, 2017: Analytical Chemistry
Tomoyuki Fujii, Akira Oka, Ichiro Morioka, Hiroyuki Moriuchi, Shin Koyano, Hideto Yamada, Shigeru Saito, Hiroshi Sameshima, Takeshi Nagamatsu, Shinya Tsuchida, Naoki Inoue
OBJECTIVES: To establish a strategy for congenital cytomegalovirus (cCMV) screening and to establish confirmatory assays approved as In Vitro diagnostics (IVD) by the regulatory authorities, we evaluated the clinical risks and performance of diagnostic assays developed by commercial companies, since cCMV infection has significant clinical consequences. STUDY DESIGN: Newborns with clinical manifestations considered to be consequences of cCMV infection (n=575) were screened for the presence of CMV DNA in urine specimens collected onto filter paper placed in their diapers using the PCR-based assay reported previously...
May 3, 2017: Pediatric Infectious Disease Journal
Ron VAN DER Oost, Giulia Sileno, Theo Janse, Mai Thao Nguyen, Harrie Besselink, Abraham Brouwer
Since it is impossible to chemically analyze all relevant micropollutants, the implementation of bioanalytical tools is essential to estimate ecological risks of chemical mixtures in regular water monitoring programs. The first tier of the Smart Integrated Monitoring (SIMONI) strategy, which was described in part I, is based on the combination of passive sampling and bioanalytical measurements. Bioassay responses are compared to effect-based trigger values (EBT) and an overall SIMONI score on all bioassay data was designed to indicate environmental risks...
May 4, 2017: Environmental Toxicology and Chemistry
Hui Jiang, Rong Ma, Shubiao Zou, Yongzhong Wang, Zhuqing Li, Weiping Li
Rheumatoid arthritis (RA) is an autoimmune disease with an unknown etiology, occurring in approximately 1.0% of general population. More and more studies have suggested that long non-coding RNAs (lncRNAs) could play important roles in various biological processes and be associated with the pathogenesis of different kinds of diseases including RA. Although a large number of lncRNAs have been found, our knowledge of their function and physiological/pathological significance is still in its infancy. In order to reveal functional lncRNAs and identify the key lncRNAs in RA, we reconstructed a global triple network based on the competitive endogenous RNA (ceRNA) theory using the data from National Center for Biotechnology Information Gene Expression Omnibus and our previous paper...
May 4, 2017: Molecular BioSystems
Thomas Schneider, Martin Westermann, Michael Glei
In this paper, we investigated the toxicological behavior of metal nanoparticles (gold, silver) and metal oxide nanoparticles (copper oxide, zinc oxide, titanium dioxide) in vitro in human colorectal adenocarcinoma cells (HT29). We analyzed the cellular uptake by ICP-MS and TEM, the influence on cell viability by MTT assay and trypan blue exclusion test, their effect on DNA damage and/or generation of oxidized bases by alkaline comet assay, and their potential to induce apoptosis by flow cytometry after 24-h nanoparticle treatment with concentrations between 2 and 10 µg/ml...
May 2, 2017: Archives of Toxicology
Yun Xing, Xiao-Zhen Feng, Lipeng Zhang, Jiating Hou, Guo-Cheng Han, Zhencheng Chen
Soluble beta-amyloid (Aβ) oligomer is believed to be the most important toxic species in the brain of Alzheimer's disease (AD) patients. Thus, it is critical to develop a simple method for the selective detection of Aβ oligomer with low cost and high sensitivity. In this paper, we report an electrochemical method for the detection of Aβ oligomer with a peptide as the bioreceptor and silver nanoparticle (AgNP) aggregates as the redox reporters. This strategy is based on the conversion of AgNP-based colorimetric assay into electrochemical analysis...
2017: International Journal of Nanomedicine
Lyudmyla G Glushakova, Barry W Alto, Myong Sang Kim, Andrea Bradley, Ozlem Yaren, Steven A Benner
Chikungunya virus (CHIKV) represents a growing and global concern for public health that needs inexpensive and convenient methods to collect mosquitoes as potential carriers so that they can be preserved, stored and transported for later and/or remote analysis. Reported here is a cellulose-based paper, derivatized with quaternary ammonium groups ("Q-paper") that meets these needs. In a series of tests, infected mosquito bodies were squashed directly on Q-paper. Aqueous ammonia was then added on the mosquito bodies to release viral RNA that adsorbed on the cationic surface via electrostatic interactions...
April 27, 2017: Journal of Virological Methods
Marek Kozinski, Magdalena Krintus, Jacek Kubica, Grazyna Sypniewska
Implementation of cardiac troponin (cTn) assays has revolutionized the diagnosis, risk stratification, triage and management of patients with suspected myocardial infarction (MI). The Universal Definition of MI brought about a shift in the diagnostics of MI, from an approach primarily based on electrocardiography (ECG) to one primarily based on biomarkers. Currently, detection of a rise and/or fall in concentration or activity of myocardial necrosis biomarkers, preferentially cTns, with at least one value above the 99th percentile upper reference limit (URL), is the essential component for the diagnosis of MI...
May 2017: Critical Reviews in Clinical Laboratory Sciences
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