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https://www.readbyqxmd.com/read/29338152/generation-and-characterization-of-siglec-f-specific-monoclonal-antibodies
#1
Sima Shahmohammadi-Farid, Roya Ghods, Mahmood Jeddi-Tehrani, Ali-Ahmad Bayat, Nazanin Mojtabavi, Alireza Razavi, Amir-Hassan Zarnani
Siglec-F (SF) is a surface glycoprotein expressed by mouse eosinophils and induces caspase- and mitochondria-dependent apoptosis after engagement with its cognate ligand or specific antibodies. This targeting eosinophils by monoclonal antibodies may help diverse diseases associated with increased frequency of eosinophils including allergy and asthma. In this paper, production of murine and rat monoclonal antibodies (mAbs) against Siglec-F has been addressed. Balb/c mice were immunized with siglec-F1 (SF1) and siglec-F2 (SF2) synthetic peptides conjugated to a carrier protein...
December 2017: Iranian Journal of Allergy, Asthma, and Immunology
https://www.readbyqxmd.com/read/29337224/an-experimental-study-for-rapid-detection-and-quantification-of-endodontic-microbiota-following-photo-activated-disinfection-via-new-multiplex-real-time-pcr-assay
#2
Maryam Pourhajibagher, Reza Raoofian, Roghayeh Ghorbanzadeh, Abbas Bahador
BACKGROUND: The infected root canal system harbors one of the highest accumulations of polymicrobial infections. Since the eradication of endopathogenic microbiota is a major goal in endodontic infection therapy, photo-activated disinfection (PAD) can be used as an alternative therapeutic method in endodontic treatment. Compared to cultivation-based approaches, molecular techniques are more reliable for identifying microbial agents associated with endodontic infections. The purpose of this study was to evaluate the ability of designed multiplex real-time PCR protocol for the rapid detection and quantification of six common microorganisms involved in endodontic infection before and after the PAD...
January 11, 2018: Photodiagnosis and Photodynamic Therapy
https://www.readbyqxmd.com/read/29336725/adaptability-of-mitosporic-stage-in-sphaerodes-mycoparasitica-towards-its-mycoparasitic-polyphagous-lifestyle
#3
Vladimir Vujanovic, Seon Hwa Kim
Sphaerodes mycoparasitica Vuj. is a Fusarium-specific mycoparasite. Some recent discoveries recognize its biotrophic polyphagous lifestyle as an interesting biocontrol property against a broad spectrum of mycotoxigenic Fusarium hosts. Secondary metabolites such as mycotoxins produced by Fusarium spp. may play an important role in the signaling process, allowing an early mycoparasite-host recognition. A multiple-paper-disc assay has been conducted to test S. mycoparasitica hyphal adaptability to filtrates of 12 Fusarium spp...
November 17, 2017: Mycologia
https://www.readbyqxmd.com/read/29335459/publisher-correction-discovery-of-targetable-genetic-alterations-in-advanced-non-small-cell-lung-cancer-using-a-next-generation-sequencing-based-circulating-tumor-dna-assay
#4
Helei Hou, Xiaonan Yang, Jinping Zhang, Zhe Zhang, Xiaomei Xu, Xiaoping Zhang, Chuantao Zhang, Dong Liu, Weihua Yan, Na Zhou, Hongmei Zhu, Zhaoyang Qian, Zhuokun Li, Xiaochun Zhang
A correction to this article has been published and is linked from the HTML version of this paper. The error has been fixed in the paper.
January 15, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29334739/microfluidic-assisted-production-of-size-controlled-spions-loaded-pmma-nanohybrids
#5
Shukai Ding, Mohamed F Attia, Justine Wallyn, Chiara Taddei, Christophe A Serra, Nicolas Anton, Mohamad Kassem, Marc Schmutz, Meriem Er-Rafik, Nadia Messaddeq, Alexandre Collard, Wei Yu, Michele Giordano, Thierry F Vandamme
In this paper, superparamagnetic iron oxide nanoparticles (SPIONs, around 6 nm) encapsulated in poly(methyl methacrylate) nanoparticles (PMMA NPs) with controlled sizes ranging from 100 nm to 200 nm have been successfully produced. The hybrid polymeric nanoparticles were prepared following two different methods: (1) nanoprecipitation and (2) nanoemulsification-evaporation. These two methods were implemented in two different microprocesses based on the use of an impact jet micromixer and an elongational-flow microemulsifier respectively...
January 16, 2018: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/29332836/fluorescent-and-colorimetric-dual-mode-aptasensor-for-thrombin-detection-based-on-target-induced-conjunction-of-split-aptamer-fragments
#6
Wenna Duan, Xiuzhong Wang, Haixia Wang, Feng Li
Since the lack of detection diversity of the single-signal readout strategy, it is urgent to develop fast and multisignal assay strategies. A highly selective and sensitive assay method with colorimetric and fluorometric dual signals readouts is presented in this paper. It is based on the principle that the target induced conjunction of split aptamer fragments assembled on the surface of Au nanoparticles (AuNPs). In the presence of targets, the color of solution changed from wine red to blue and can be measured both visual inspection and spectrophotometry because of the aggregation of AuNPs...
April 1, 2018: Talanta
https://www.readbyqxmd.com/read/29332792/silver-ions-enhanced-auncs-fluorescence-as-a-turn-off-nanoprobe-for-ultrasensitive-detection-of-iodide
#7
Wenli Hou, Yuan Chen, Qiujun Lu, Meiling Liu, Youyu Zhang, Shouzhuo Yao
Fluorescence nanoprobes are frequently employed to construct sensitive biosensors via turn-on and turn-off strategy. In this paper, a novel strategy for ultrasensitive detection of iodide was firstly constructed based on Ag+ regulated photoluminescence enhancement of gold nanoclusters (AuNCs) as a turn-off nanoplatform. In the presence of Ag+, the fluorescence (FL) intensity of AuNCs can be enhanced obviously. When adding iodide ions (I-) in the Ag+-AuNCs, Ag+ can be pulled down from AuNCs and results in quenching of the fluorescent effectively owing to the combination between Ag+ and I-...
April 1, 2018: Talanta
https://www.readbyqxmd.com/read/29331429/paper-based-fluorescent-sensor-via-aggregation-induced-emission-fluorogen-for-facile-and-sensitive-visual-detection-of-hydrogen-peroxide-and-glucose
#8
Jiafu Chang, Haiyin Li, Ting Hou, Wenna Duan, Feng Li
Hydrogen peroxide (H2O2), an important reactive oxygen species (ROS), is related to the oxidative stress in organisms, and plays important roles in a variety of cellular activities as well. So it is of crucial importance to develop sensitive and accurate sensing strategies to detect H2O2 in biological systems. Herein, by taking advantage of the unique emission characteristics of aggregation induced emission (AIE) fluorogens, we proposed a non-enzymatic fluorescence platform for facile and sensitive detection of H2O2, both in solution state using fluorescence spectrometer and on paper-based sensor via visual inspection...
January 6, 2018: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29330381/paper-based-inkjet-bioprinting-to-detect-fluorescence-resonance-energy-transfer-for-the-assessment-of-anti-inflammatory-activity
#9
Annie Agnes Suganya Samson, Jungmi Lee, Joon Myong Song
For the first time, a paper-based fluorescence resonance energy transfer (FRET) determination with cyclic AMP (cAMP)-specific phosphodiesterase 4B (PDE4B) inhibitory assay using an inkjet-printing technique is proposed. Non-fabricated parchment paper is found to constitute a unique substrate to measure fluorescent energy transfer, due to its insignificant self-absorption, and enables efficient sample interaction. Here, we report the responsive FRET signals generated on paper, upon sequentially printing reaction components on parchment paper using a conventional inkjet printer equipped with four cartridges...
January 12, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29326990/a-fluorescent-microbead-based-microfluidic-immunoassay-chip-for-immune-cell-cytokine-secretion-quantification
#10
Xin Cui, Ya Liu, Dinglong Hu, Weiyi Qian, Chung Tin, Dong Sun, Weiqiang Chen, Raymond H W Lam
Quantitative and dynamic analyses of immune cell secretory cytokines are essential for precise determination and characterization of the "immune phenotype" of patients for clinical diagnosis and treatment of immune-related diseases. Although multiple methods including the enzyme-linked immunosorbent assay (ELISA) have been applied for cytokine detection, such measurements remain very challenging in real-time, high-throughput, and high-sensitivity immune cell analysis. In this paper, we report a highly integrated microfluidic device that allows for on-chip isolation, culture, and stimulation, as well as sensitive and dynamic cytokine profiling of immune cells...
January 12, 2018: Lab on a Chip
https://www.readbyqxmd.com/read/29323478/simultaneous-detection-of-antibiotic-resistance-genes-on-paper-based-chip-using-ru-phen-2dppz-2-turn-on-fluorescence-probe
#11
Bofan Li, Xiaoming Zhou, Hongxing Liu, Huaping Deng, Ru Huang, Da Xing
Antibiotic resistance, the ability of some bacteria to resist antibiotic drug, has been a major global health burden due to the extensive use of antibiotic agents. Antibiotic resistance is encoded via respective genes, hence the specific detection of these genes is necessary for diagnostic and treat of antibiotic resistance cases. Conventional methods for monitoring antibiotic resistance genes require sample to be transported to a central laboratory for tedious and sophisticated tests, which is grueling and time-consuming...
January 11, 2018: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29321466/an-instrument-free-detection-of-antioxidant-activity-using-paper-based-analytical-devices-coated-with-nanoceria
#12
Thirada Piyanan, Anan Athipornchai, Charles S Henry, Yupaporn Sameenoi
This work reports a portable distance-based detection paper device that has a thermometer-like shape for rapid, instrument-free determination of antioxidant activity using a nanoceria assay. The assay is based on partial reduction of cerium ion from Ce4+ to Ce3+ on nanoceria deposited along the detection channel by antioxidants present in food, giving highly reactive oxidation products. Either these products or the parent antioxidant compounds could then bind to the OH-rich ceria nanoparticles and generate charge transfer ceria-antioxidant complexes resulting in a yellow to brown color change...
2018: Analytical Sciences: the International Journal of the Japan Society for Analytical Chemistry
https://www.readbyqxmd.com/read/29321462/microfluidic-paper-based-analytical-device-for-the-determination-of-hexavalent-chromium-by-photolithographic-fabrication-using-a-photomask-printed-with-3d-printer
#13
Hitoshi Asano, Yukihide Shiraishi
This article describes a simple and inexpensive microfluidic paper-based analytical device (μPAD) for the determination of hexavalent chromium (CrVI) in water samples. The μPADs were fabricated on paper by photolithography using a photomask printed with a 3D printer and functionalized with reagents for a colorimetric assay. In the μPAD, CrVI reacts with 1,5-diphenylcarbazide to form a violet-colored complex. Images of μPADs were captured with a digital camera; then the red, green, and blue color intensity of each detection zone were measured using images processing software...
2018: Analytical Sciences: the International Journal of the Japan Society for Analytical Chemistry
https://www.readbyqxmd.com/read/29321459/practical-high-performance-lateral-flow-assay-based-on-autonomous-microfluidic-replacement-on-a-film
#14
Yusuke Fuchiwaki, Kenji Goya, Masato Tanaka
Although paper-based microfluidic devices are an ideal platform for point-of-care (POC) diagnostics, it is difficult to achieve microfluidic control required for sensitive analyses such as ELISA on a paper substrate. Here, we present a novel lateral-flow test chip that can perform operations similar to a pump, such as flowing, stopping, and replacing a solution, just by adding the solution onto an inlet port. The chip was fabricated by laminating paper, film, and adhesive tape. For sensitive and accurate detection in an immunoassay, the transparency and flatness of the substrate is crucial for precise analysis of weak light generated by a specific antigen-antibody reaction; however, paper is not flat and is opaque...
2018: Analytical Sciences: the International Journal of the Japan Society for Analytical Chemistry
https://www.readbyqxmd.com/read/29321458/wax-assisted-one-step-enzyme-linked-immunosorbent-assay-on-lateral-flow-test-devices
#15
Masanori Ishii, Pattarachaya Preechakasedkit, Kentaro Yamada, Orawon Chailapakul, Koji Suzuki, Daniel Citterio
Lateral flow tests (LFTs) are widely used analytical tools characterized by portability, operator simplicity and short analysis times. A remaining challenge is their limited analytical sensitivity, which in classical immunoassay formats is overcome by enzyme-linked immunosorbent assay (ELISA) formats. The implementation of ELISA to an LFT format however, is hampered by the complexity of the procedure requiring the enzyme substrate addition after sample addition. In this work, a simple method for automation of this procedure without user interference is presented...
2018: Analytical Sciences: the International Journal of the Japan Society for Analytical Chemistry
https://www.readbyqxmd.com/read/29321449/a-simple-paper-based-colorimetric-device-for-rapid-and-sensitive-urinary-oxalate-determinations
#16
Piyakorn Worramongkona, Kanyarat Seeda, Phatharatanathorn Phansomboon, Nalin Ratnarathorn, Orawon Chailapakul, Wijitar Dungchai
In this work, a simple and inexpensive paper-based colorimetric device (cPAD) was developed for oxalate measurements. The colorimetric assay is based on the formation of formazan via the reduction of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) by oxalate decarboxylase and formate dehydrogenase on the paper device. After the sample was spotted on the device, MTT changed color from yellow to purple, and the purple color intensity correlated with the oxalate concentration. The quantitative detection of oxalate ranged from 10 - 1000 μM, with a linear equation, y = 0...
2018: Analytical Sciences: the International Journal of the Japan Society for Analytical Chemistry
https://www.readbyqxmd.com/read/29317167/sirt1-activator-isolated-from-artificial-gastric-juice-incubate-of-total-saponins-in-stems-and-leaves-of-panax-ginseng
#17
Xiu-Wei Yang, Li-Yuan Ma, Qi-Le Zhou, Wei Xu, You-Bo Zhang
Panax ginseng as a traditional Chinese medicine has been extensively used for the treatment of many diseases, especially in prolonging life and anti-tumor. Dammarane-type triterpenoids from P. ginseng have diverse beneficial effects and their chemical structures can be modified in the gastrointestinal tract after oral administration. In this paper, the dammarane-type triterpenoids were isolated from artificial gastric juice incubate of total saponins in the stems and leaves of P. ginseng through column chromatographic methods and their chemical structures were determined based on spectral data...
December 30, 2017: Bioorganic & Medicinal Chemistry Letters
https://www.readbyqxmd.com/read/29316529/vancomycin-assisted-green-synthesis-of-reduced-graphene-oxide-for-antimicrobial-applications
#18
Li Qun Xu, Yan Biao Liao, Ning Ning Li, Yi Jian Li, Jie Yu Zhang, Yun Bing Wang, Xue Feng Hu, Chang Ming Li
Chemical reduction of graphene oxide (GO) is a simple and inexpensive method for the large-scale production of graphene-based materials. A suitable reducing agent, especially a green reductant, is in high demand for the production of reduced GO (RGO). Vancomycin, a glycopeptide antibiotic used to treat a variety of Gram-positive bacterial infections, was used to chemically reduce GO at a weak alkaline pH. As far as we know, this is the first report of reduction of GO by a glycopeptide antibiotic. The resulting vancomycin-decorated RGO (RGO-Van) was characterized by UV-visible adsorption and Raman spectroscopies, X-ray diffraction pattern and atomic force microscopy (AFM)...
January 4, 2018: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/29315269/mining-the-potential-of-label-free-biosensors-for-in-vitro-antipsychotic-drug-screening
#19
Tugba Kilic, Maria Soler, Nafiseh Fahimi-Kashani, Hatice Altug, Sandro Carrara
The pharmaceutical industry is facing enormous challenges due to high drug attribution rates. For the past decades, novel methods have been developed for safety and efficacy testing, as well as for improving early development stages. In vitro screening methods for drug-receptor binding are considered to be good alternatives for decreasing costs in the identification of drug candidates. However, these methods require lengthy and troublesome labeling steps. Biosensors hold great promise due to the fact that label-free detection schemes can be designed in an easy and low-cost manner...
January 9, 2018: Biosensors
https://www.readbyqxmd.com/read/29310287/a-novel-enzyme-linked-immunosorbent-assay-based-on-the-catalysis-of-auncs-bsa-induced-signal-amplification-for-the-detection-of-dibutyl-phthalate
#20
Zhen Zhang, Nuanfei Zhu, Yanmin Zou, Xiangyang Wu, Guangbo Qu, Jianbo Shi
A novel, competitive, enzyme-linked immunosorbent assay (ELISA) was presented in this paper based on the inhibition of catalysis of AuNCs@BSA triggered by dissolved Ag+ for the detection of dibutyl phthalate (DBP). In the immunoassay system, numerous Ag+ was released from AgNPs (labelled on the second antibody, AgNPs@Ab2) in the presence of H2O2 after the competition step, preventing AuNCs@BSA from inducing a color change of 3,3',5,5'-tetramethylbenzidine (TMB) to blue. Due to the signal amplification by the principle, the sensitivity of the modified ELISA was improved with the low limit of detection (LOD) of 4...
March 1, 2018: Talanta
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