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https://www.readbyqxmd.com/read/28936177/withaferin-a-induces-oxidative-stress-mediated-apoptosis-and-dna-damage-in-oral-cancer-cells
#1
Hsueh-Wei Chang, Ruei-Nian Li, Hui-Ru Wang, Jing-Ru Liu, Jen-Yang Tang, Hurng-Wern Huang, Yu-Hsuan Chan, Ching-Yu Yen
Withaferin A (WFA) is one of the most active steroidal lactones with reactive oxygen species (ROS) modulating effects against several types of cancer. ROS regulation involves selective killing. However, the anticancer and selective killing effects of WFA against oral cancer cells remain unclear. We evaluated whether the killing ability of WFA is selective, and we explored its mechanism against oral cancer cells. An MTS tetrazolium cell proliferation assay confirmed that WFA selectively killed two oral cancer cells (Ca9-22 and CAL 27) rather than normal oral cells (HGF-1)...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/28931748/participation-of-bmi-1-protein-in-cancer
#2
Agnieszka Zaczek, Paweł Jóźwiak, Anna Krześlak
BMI-1 (B-lymphoma Mo-MLV insertion region 1) protein is a constituent of Polycomb Repressive Complex 1 (PRC1) that via ubiquitination of histone H2A affects expression of many genes. BMI-1 is involved in cellular processes such as DNA repair, proliferation, growth, senescence and apoptosis. BMI-1 plays a key role in biology of stem cells including cancer stem cells by regulation of their self-renewal and differentiation. Accumulating evidence has revealed that overexpression of BMI-1 in many human cancers correlates with disease progression and therapy failure...
September 20, 2017: Postȩpy Higieny i Medycyny Doświadczalnej
https://www.readbyqxmd.com/read/28928118/know-your-histone-zip-code-flowering-time-and-phosphorylation-of-histone-h2a-on-serine-95
#3
Patrice A Salomé
No abstract text is available yet for this article.
September 19, 2017: Plant Cell
https://www.readbyqxmd.com/read/28925810/ube3a-mediated-regulation-of-imprinted-genes-and-epigenome-wide-marks-in-human-neurons
#4
S Jesse Lopez, Keith Dunaway, M Saharul Islam, Charles Mordaunt, Annie Vogel Ciernia, Makiko Meguro-Horike, Shin-Ichi Horike, David J Segal, Janine LaSalle
The dysregulation of genes in neurodevelopmental disorders that lead to social and cognitive phenotypes is a complex, multilayered process involving both genetics and epigenetics. Parent-of-origin effects of deletion and duplication of the 15q11-q13 locus leading to Angelman, Prader-Willi, and Dup15q syndromes are due to imprinted genes, including UBE3A, which is maternally expressed exclusively in neurons. UBE3A encodes a ubiquitin E3 ligase protein with multiple downstream targets, including RING1B, which in turn monoubiquitinates histone variant H2A...
September 19, 2017: Epigenetics: Official Journal of the DNA Methylation Society
https://www.readbyqxmd.com/read/28922417/lingering-single-strand-breaks-trigger-rad51-independent-homology-directed-repair-of-collapsed-replication-forks-in-the-polynucleotide-kinase-phosphatase-mutant-of-fission-yeast
#5
Arancha Sanchez, Mariana C Gadaleta, Oliver Limbo, Paul Russell
The DNA repair enzyme polynucleotide kinase/phosphatase (PNKP) protects genome integrity by restoring ligatable 5'-phosphate and 3'-hydroxyl termini at single-strand breaks (SSBs). In humans, PNKP mutations underlie the neurological disease known as MCSZ, but these individuals are not predisposed for cancer, implying effective alternative repair pathways in dividing cells. Homology-directed repair (HDR) of collapsed replication forks was proposed to repair SSBs in PNKP-deficient cells, but the critical HDR protein Rad51 is not required in PNKP-null (pnk1Δ) cells of Schizosaccharomyces pombe...
September 18, 2017: PLoS Genetics
https://www.readbyqxmd.com/read/28919888/the-sporothrix-schenckii-gene-encoding-for-the-ribosomal-protein-l6-has-constitutive-and-stable-expression-and-works-as-an-endogenous-control-in-gene-expression-analysis
#6
Elías Trujillo-Esquivel, José A Martínez-Álvarez, Diana M Clavijo-Giraldo, Nahúm V Hernández, Alberto Flores-Martínez, Patricia Ponce-Noyola, Héctor M Mora-Montes
Sporothrix schenckii is one of the causative agents of sporotrichosis, a worldwide-distributed mycosis that affects humans and other mammals. The interest in basic and clinical features of this organism has significantly increased in the last years, yet little progress in molecular aspects has been reported. Gene expression analysis is a set of powerful tools that helps to assess the cell response to changes in the extracellular environment, the genetic networks controlling metabolic pathways, and the adaptation to different growth conditions...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28913935/induction-of-cancer-cell-stemness-by-depletion-of-macrohistone-h2a1-in-hepatocellular-carcinoma
#7
Oriana Lo Re, Caterina Fusilli, Francesca Rappa, Matthias Van Haele, Julien Douet, Jana Pindjakova, Sura Wanessa Rocha, Illar Pata, Barbora Valčíková, Stjepan Uldrijan, Raymond S Yeung, Christina Alves Peixoto, Tania Roskams, Marcus Buschbeck, Tommaso Mazza, Manlio Vinciguerra
Hepatocellular carcinomas (HCC) contain a sub-population of cancer stem cells (CSCs), which exhibit stem-cell like features and are responsible for tumor relapse, metastasis, and chemoresistance. The development of effective treatments for HCC will depend on a molecular-level understanding of the specific pathways driving CSC emergence and stemness. MacroH2A1 is a variant of the histone H2A and an epigenetic regulator of stem cell function, where it promotes differentiation and, conversely, acts as a barrier to somatic cell reprogramming...
September 15, 2017: Hepatology: Official Journal of the American Association for the Study of Liver Diseases
https://www.readbyqxmd.com/read/28898261/aged-blood-factors-decrease-cellular-responses-associated-with-delayed-gingival-wound-repair
#8
María Paz Saldías, Christian Fernández, Alejandra Morgan, Catalina Díaz, Diego Morales, Fabián Jaña, Alvaro Gómez, Alonso Silva, Fernanda Briceño, Alejandro Oyarzún, Felipe Maldonado, Oscar Cerda, Patricio C Smith, Mónica Cáceres
Aging is a gradual biological process characterized by a decrease in cell and organism functions. Gingival wound healing is one of the impaired processes found in old rats. Here, we studied the in vivo wound healing process using a gingival repair rat model and an in vitro model using human gingival fibroblast for cellular responses associated to wound healing. To do that, we evaluated cell proliferation of both epithelial and connective tissue cells in gingival wounds and found decreased of Ki67 nuclear staining in old rats when compared to their young counterparts...
2017: PloS One
https://www.readbyqxmd.com/read/28894150/melatonin-enhances-the-developmental-competence-of-porcine-somatic-cell-nuclear-transfer-embryos-by-preventing-dna-damage-induced-by-oxidative-stress
#9
Shuang Liang, Yong-Xun Jin, Bao Yuan, Jia-Bao Zhang, Nam-Hyung Kim
Melatonin has antioxidant and scavenger effects in the cellular antioxidant system. This research investigated the protective effects and underlying mechanisms of melatonin action in porcine somatic cell nuclear transfer (SCNT) embryos. The results suggested that the developmental competence of porcine SCNT embryos was considerably enhanced after melatonin treatment. In addition, melatonin attenuated the increase in reactive oxygen species levels induced by oxidative stress, the decrease in glutathione levels, and the mitochondrial dysfunction...
September 11, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28893714/transcriptional-regulation-of-the-ambient-temperature-response-by-h2a-z-nucleosomes-and-hsf1-transcription-factors-in-arabidopsis
#10
Sandra Cortijo, Varodom Charoensawan, Anna Brestovitsky, Ruth Buning, Charles Ravarani, Daniela Rhodes, John van Noort, Katja E Jaeger, Philip A Wigge
Temperature influences the distribution, range and phenology of plants. The key transcriptional activators of the heat shock response in eukaryotes, the heat shock factors (HSFs), have undergone a large-scale gene amplification in plants. While HSFs are central in heat stress responses, their role in ambient temperature changes is less well understood. We show here that the warm ambient temperature transcriptome is dependent upon the HSFA1 clade of Arabidopsis HSFs, which cause a rapid and dynamic eviction of H2A...
September 8, 2017: Molecular Plant
https://www.readbyqxmd.com/read/28893254/skip-controls-flowering-time-via-the-alternative-splicing-of-sef-pre-mrna-in-arabidopsis
#11
Zhibo Cui, Aizi Tong, Yiqiong Huo, Zhiqiang Yan, Weiqi Yang, Xianli Yang, Xiao-Xue Wang
BACKGROUND: Similar to other eukaryotes, splicing is emerging as an important process affecting development and stress tolerance in plants. Ski-interacting protein (SKIP), a splicing factor, is essential for circadian clock function and abiotic stress tolerance; however, the mechanisms whereby it regulates flowering time are unknown. RESULTS: In this study, we found that SKIP is required for the splicing of serrated leaves and early flowering (SEF) pre-messenger RNA (mRNA), which encodes a component of the ATP-dependent SWR1 chromatin remodeling complex (SWR1-C)...
September 11, 2017: BMC Biology
https://www.readbyqxmd.com/read/28883625/redundant-functions-for-nap1-and-chz1-in-h2a-z-deposition
#12
Raghuvar Dronamraju, Srinivas Ramachandran, Deepak K Jha, Alexander T Adams, Julia V DiFiore, Michael A Parra, Nikolay V Dokholyan, Brian D Strahl
H2A.Z is a histone H2A variant that contributes to transcriptional regulation, DNA damage response and limits heterochromatin spreading. In Saccharomyces cerevisiae, H2A.Z is deposited by the SWR-C complex, which relies on several histone chaperones including Nap1 and Chz1 to deliver H2A.Z-H2B dimers to SWR-C. However, the mechanisms by which Nap1 and Chz1 cooperate to bind H2A.Z and their contribution to H2A.Z deposition in chromatin is not well understood. Using structural modeling and molecular dynamics simulations, we identify a series of H2A...
September 7, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28881649/identification-of-deubiquitinase-targets-of-isothiocyanates-using-silac-assisted-quantitative-mass-spectrometry
#13
Ann P Lawson, Daniel W Bak, D Alexander Shannon, Marcus J C Long, Tushara Vijaykumar, Runhan Yu, Farid El Oualid, Eranthie Weerapana, Lizbeth Hedstrom
Cruciferous vegetables such as broccoli and kale have well documented chemopreventative and anticancer effects that are attributed to the presence of isothiocyanates (ITCs). ITCs modulate the levels of many oncogenic proteins, but the molecular mechanisms of ITC action are not understood. We previously reported that phenethyl isothiocyanate (PEITC) inhibits two deubiquitinases (DUBs), USP9x and UCH37. DUBs regulate many cellular processes and DUB dysregulation is linked to the pathogenesis of human diseases including cancer, neurodegeneration, and inflammation...
August 1, 2017: Oncotarget
https://www.readbyqxmd.com/read/28871473/role-of-peroxiredoxin-2-in-the-protection-against-ferrous-sulfate-induced-oxidative-and-inflammatory-injury-in-pc12-cells
#14
Wenzhe Xu, Feng Li, Zhenkuan Xu, Bin Sun, Jingwei Cao, Yuguang Liu
Peroxiredoxin 2 (Prdx2) is a ubiquitous antioxidant enzyme in mammalian brain. Although a protective role of Prdx2 has been established in cerebral ischemia and several neurodegenerative diseases, its contribution against iron-induced neurocytotoxicity still remains to be determined. Accordingly, in this study, we aimed to investigate the effects of Prdx2 on iron-induced cytotoxicity using an in vitro model in which PC12 cells are exposed to ferrous sulfate (FS). The FS treatment increased Prdx2 expression, and promoted lactate dehydrogenase (LDH) release and cell apoptosis in PC12 cells, accompanied by the increase in the Bax/Bcl2 ratio, cytochrome c release, and caspase-3 cleavage...
September 4, 2017: Cellular and Molecular Neurobiology
https://www.readbyqxmd.com/read/28867704/evaluation-of-retinal-function-in-streptozotocin-induced-diabetic-rats-by-using-the-electroretinography-and-immunohistochemistry-methods
#15
Noriko Hiramatsu, Saori Deguchi, Chiaki Yoshioka, Hiroko Otake, Naoki Yamamoto, Noriaki Nagai
 Streptozotocin-induced diabetic rat (STZ rat) was used in many studies for the diabetic mellitus. In this study, we demonstrated whether the electroretinograms (ERG) was changed in the retina of STZ rats. In addition, we investigated the histopathological alteration in the retina of STZ rats by using the immunological method. The 100 mg/kg of STZ was injected continuously for 2 d (100 mg/kg×2). The insulin level was decreased, and the glucose level was enhanced 14 d after the injection of STZ. Moreover, the levels of a-wave, b-wave and OP amplitude were decreased in the rat at 14 d after the injection of STZ...
2017: Yakugaku Zasshi: Journal of the Pharmaceutical Society of Japan
https://www.readbyqxmd.com/read/28862252/genome-wide-identification-of-histone-h2a-and-histone-variant-h2a-z-interacting-proteins-by-bppi-seq
#16
Yi Zhang, Wai Lim Ku, Shuai Liu, Kairong Cui, Wenfei Jin, Qingsong Tang, William Lu, Bing Ni, Keji Zhao
H2A is a nucleosome core subunit involved in organizing DNA into a chromatin structure that is often inaccessible to regulatory enzymes. Replacement of H2A by its variant H2A.Z renders chromatin accessible at enhancers and promoters. However, it remains unclear how H2A.Z functions so differently from canonical H2A. Here we report the genome-wide identification of proteins that directly interact with H2A and H2A.Z in vivo using a novel strategy, bPPI-seq. We show that bPPI-seq is a sensitive and robust technique to identify protein-protein interactions in vivo...
September 1, 2017: Cell Research
https://www.readbyqxmd.com/read/28856239/histone-hypervariants-h2a-z-1-and-h2a-z-2-play-independent-and-context-specific-roles-in-neuronal-activity-induced-transcription-of-arc-arg3-1-and-other-immediate-early-genes
#17
Carissa J Dunn, Pushpita Sarkar, Emma R Bailey, Shannon Farris, Meilan Zhao, James M Ward, Serena M Dudek, Ramendra N Saha
The histone variant H2A.Z is an essential and conserved regulator of eukaryotic gene transcription. However, the exact role of this histone in the transcriptional process remains perplexing. In vertebrates, H2A.Z has two hypervariants, H2A.Z.1 and H2A.Z.2, that have almost identical sequences except for three amino acid residues. Due to such similarity, functional specificity of these hypervariants in neurobiological processes, if any, remain largely unknown. In this study with dissociated rat cortical neurons, we asked if H2A...
July 2017: ENeuro
https://www.readbyqxmd.com/read/28855339/role-of-remodeling-and-spacing-factor-1-in-histone-h2a-ubiquitination-mediated-gene-silencing
#18
Zhuo Zhang, Amanda E Jones, Wei Wu, Jinman Kim, Yue Kang, Xiaobao Bi, Yue Gu, Ivan K Popov, Matthew B Renfrow, Marina N Vassylyeva, Dmitry G Vassylyev, Keith E Giles, Dongquan Chen, Ashwath Kumar, Yuhong Fan, Yufeng Tong, Chuan-Fa Liu, Woojin An, Chenbei Chang, Jianjun Luo, Louise T Chow, Hengbin Wang
Posttranslational histone modifications play important roles in regulating chromatin-based nuclear processes. Histone H2AK119 ubiquitination (H2Aub) is a prevalent modification and has been primarily linked to gene silencing. However, the underlying mechanism remains largely obscure. Here we report the identification of RSF1 (remodeling and spacing factor 1), a subunit of the RSF complex, as a H2Aub binding protein, which mediates the gene-silencing function of this histone modification. RSF1 associates specifically with H2Aub, but not H2Bub nucleosomes, through a previously uncharacterized and obligatory region designated as ubiquitinated H2A binding domain...
August 30, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28855255/single-molecule-force-spectroscopy-on-histone-h4-tail-cross-linked-chromatin-reveals-fiber-folding
#19
Artur Kaczmarczyk, Abdollah Allahverdi, Thomas B Brouwer, Lars Nordenskiöld, Nynke H Dekker, John van Noort
The eukaryotic genome is highly compacted into a protein-DNA complex called chromatin. The cell controls access of transcriptional regulators to chromosomal DNA via several mechanisms that act on chromatin-associated proteins and provide a rich spectrum of epigenetic regulation. Elucidating the mechanisms that fold chromatin fibers into higher-order structures is therefore key to understanding the epigenetic regulation of DNA accessibility. Here, using histone H4-V21C and histone H2A-E64C mutations, we employed single-molecule force spectroscopy to measure the unfolding of individual chromatin fibers that are reversibly cross-linked through the histone H4 tail...
August 30, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28854417/effects-of-blue-light-emitting-diode-irradiation-on-the-proliferation-apoptosis-and-differentiation-of-bone-marrow-derived-mesenchymal-stem-cells
#20
Ye Yuan, Gege Yan, Rui Gong, Lai Zhang, Tianyi Liu, Chao Feng, Weijie Du, Ying Wang, Fan Yang, Yuan Li, Shuyuan Guo, Fengzhi Ding, Wenya Ma, Elina Idiiatullina, Valentin Pavlov, Zhenbo Han, Benzhi Cai, Lei Yang
BACKGROUND/AIMS: Blue light emitting diodes (LEDs) have been proven to affect the growth of several types of cells. The effects of blue LEDs have not been tested on bone marrow-derived mesenchymal stem cells (BMSCs), which are important for cell-based therapy in various medical fields. Therefore, the aim of this study was to determine the effects of blue LED on the proliferation, apoptosis and osteogenic differentiation of BMSCs. METHODS: BMSCs were irradiated with a blue LED light at 470 nm for 1 min, 5 min, 10 min, 30 min and 60 min or not irradiated...
August 30, 2017: Cellular Physiology and Biochemistry
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