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https://www.readbyqxmd.com/read/29753771/mir-199a-3p-regulates-brown-adipocyte-differentiation-through-mtor-signaling-pathway
#1
Yao Gao, Yan Cao, Xianwei Cui, Xingyun Wang, Yahui Zhou, Fangyan Huang, Xing Wang, Juan Wen, Kaipeng Xie, Pengfei Xu, Xirong Guo, Lianghui You, Chenbo Ji
Recent discoveries of functional brown adipocytes in mammals illuminates their therapeutic potential for combating obesity and its associated diseases. However, on account of the limited amount and activity in adult humans of brown adipocyte depots, identification of miRNAs and characterization their regulatory roles in human brown adipogenesis are urgently needed. This study focused on the role of microRNA (miR)-199a-3p in human brown adipocyte differentiation and thermogenic capacity. A decreased expression pattern of miR-199a-3p was consistently observed during the differentiation course of brown adipocytes in mice and humans...
May 10, 2018: Molecular and Cellular Endocrinology
https://www.readbyqxmd.com/read/29745990/an-il-10-dominant-polarization-of-monocytes-is-a-feature-of-indian-visceral-leishmaniasis
#2
Susmita Roy, Debanjan Mukhopadhyay, Shibabrata Mukherjee, Srija Moulik, Soumyadip Chatterji, Nayan Brahme, Netai Pramanik, Rama P Goswami, Bibhuti Saha, Mitali Chatterjee
BACKGROUND: Leishmania donovani, the causative parasite of Visceral Leishmaniasis (VL) deviously manipulates host monocytes/macrophages to ensure its survival. Although monocytes/macrophages from patients with VL have demonstrated an impaired oxidative burst and antigen presentation, an unanswered yet pertinent question remains as to whether they are deactivated or alternatively activated. METHODS AND RESULTS: The significantly raised plasma levels of IL-4/IL-13 and IL-10 in VL patients suggested a microenvironment conducive for alternative activation of monocytes/macrophages...
May 10, 2018: Parasite Immunology
https://www.readbyqxmd.com/read/29695257/comparative-omics-and-feeding-manipulations-in-chicken-indicate-a-shift-of-the-endocrine-role-of-visceral-fat-towards-reproduction
#3
Susanne Bornelöv, Eyal Seroussi, Sara Yosefi, Sharon Benjamini, Shoval Miyara, Mark Ruzal, Manfred Grabherr, Nima Rafati, Anna-Maja Molin, Ken Pendavis, Shane C Burgess, Leif Andersson, Miriam Friedman-Einat
BACKGROUND: The mammalian adipose tissue plays a central role in energy-balance control, whereas the avian visceral fat hardly expresses leptin, the key adipokine in mammals. Therefore, to assess the endocrine role of adipose tissue in birds, we compared the transcriptome and proteome between two metabolically different types of chickens, broilers and layers, bred towards efficient meat and egg production, respectively. RESULTS: Broilers and layer hens, grown up to sexual maturation under free-feeding conditions, differed 4...
April 26, 2018: BMC Genomics
https://www.readbyqxmd.com/read/29672421/human-versus-robot-a-propensity-matched-analysis-of-the-accuracy-of-free-hand-versus-robotic-guidance-for-placement-of-s2-alar-iliac-s2ai-screws
#4
Jamal N Shillingford, Joseph L Laratta, Paul J Park, Joseph M Lombardi, Alexander Tuchman, Comron S Saifi, Ronald A Lehman, Lawrence G Lenke
STUDY DESIGN: Retrospective matched cohort analysis. OBJECTIVE: To compare the accuracy of S2 alar-iliac (S2AI) screw placement by robotic guidance versus free hand technique. SUMMARY OF BACKGROUND DATA: Spinopelvic fixation utilizing S2AI screws provides optimal fixation across the lumbosacral junction allowing for solid fusion, especially in long segment fusion constructs. Traditionally, S2AI screw placement has required fluoroscopic guidance for accurate screw placement...
April 18, 2018: Spine
https://www.readbyqxmd.com/read/29608175/quantification-of-intracellular-growth-inside-macrophages-is-a-fast-and-reliable-method-for-assessing-the-virulence-of-leishmania-parasites
#5
Amrita Sarkar, Yousuf A Khan, Maria Fernanda Laranjeira-Silva, Norma W Andrews, Bidyottam Mittra
The lifecycle of Leishmania, the causative agent of leishmaniasis, alternates between promastigote and amastigote stages inside the insect and vertebrate hosts, respectively. While pathogenic symptoms of leishmaniasis can vary widely, from benign cutaneous lesions to highly fatal visceral disease forms depending on the infective species, all Leishmania species reside inside host macrophages during the vertebrate stage of their lifecycle. Leishmania infectivity is therefore directly related to its ability to invade, survive and replicate within parasitophorous vacuoles (PVs) inside macrophages...
March 16, 2018: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29557586/flexible-robotic-catheters-in-the-visceral-segment-of-the-aorta-advantages-and-limitations
#6
Mimi M Li, Mohamad S Hamady, Colin D Bicknell, Celia V Riga
Flexible robotic catheters are an emerging technology which provide an elegant solution to the challenges of conventional endovascular intervention. Originally developed for interventional cardiology and electrophysiology procedures, remotely steerable robotic catheters such as the Magellan system enable greater precision and enhanced stability during target vessel navigation. These technical advantages facilitate improved treatment of disease in the arterial tree, as well as allowing execution of otherwise unfeasible procedures...
March 19, 2018: Journal of Cardiovascular Surgery
https://www.readbyqxmd.com/read/29499728/the-effect-of-a-six-week-osteopathic-visceral-manipulation-in-patients-with-non-specific-chronic-low-back-pain-and-functional-constipation-study-protocol-for-a-randomized-controlled-trial
#7
Walkyria Vilas Boas Fernandes, Cleofás Rodríguez Blanco, Fabiano Politti, Fernanda de Cordoba Lanza, Paulo Roberto Garcia Lucareli, João Carlos Ferrari Corrêa
BACKGROUND: The aim of the proposed study is to analyze the effect of a six-week osteopathic visceral manipulation (OVM) program on the flexion-relaxation phenomenon in individuals with non-specific chronic low back pain (LBP) and functional constipation. METHODS/DESIGN: An assessor-blinded, two-arm, randomized, placebo-controlled trial will be conducted. The sample will comprise 76 individuals with non-specific chronic LBP who have functional intestinal constipation, aged 18-65 years...
March 2, 2018: Trials
https://www.readbyqxmd.com/read/29497032/de-novo-adipocyte-differentiation-from-pdgfr%C3%AE-preadipocytes-protects-against-pathologic-visceral-adipose-expansion-in-obesity
#8
Mengle Shao, Lavanya Vishvanath, Napoleon C Busbuso, Chelsea Hepler, Bo Shan, Ankit X Sharma, Shiuhwei Chen, Xinxin Yu, Yu A An, Yi Zhu, William L Holland, Rana K Gupta
Pathologic expansion of white adipose tissue (WAT) in obesity is characterized by adipocyte hypertrophy, inflammation, and fibrosis; however, factors triggering this maladaptive remodeling are largely unknown. Here, we test the hypothesis that the potential to recruit new adipocytes from Pdgfrβ+ preadipocytes determines visceral WAT health in obesity. We manipulate levels of Pparg, the master regulator of adipogenesis, in Pdgfrβ+ precursors of adult mice. Increasing the adipogenic capacity of Pdgfrβ+ precursors through Pparg overexpression results in healthy visceral WAT expansion in obesity and adiponectin-dependent improvements in glucose homeostasis...
March 1, 2018: Nature Communications
https://www.readbyqxmd.com/read/29490092/blueberry-supplementation-influences-the-gut-microbiota-inflammation-and-insulin-resistance-in-high-fat-diet-fed-rats
#9
Sunhye Lee, Katherine I Keirsey, Rebecca Kirkland, Zachary I Grunewald, Joan G Fischer, Claire B de La Serre
Background: Gut microbiota dysbiosis has been linked to obesity-associated chronic inflammation. Microbiota manipulation may therefore affect obesity-related comorbidities. Blueberries are rich in anthocyanins, which have anti-inflammatory properties and may alter the gut microbiota. Objective: We hypothesized that blueberry supplementation would alter the gut microbiota, reduce systemic inflammation, and improve insulin resistance in high-fat (HF)-diet-fed rats...
February 1, 2018: Journal of Nutrition
https://www.readbyqxmd.com/read/29462037/the-free-hand-technique-for-s2-alar-iliac-screw-placement-a-safe-and-effective-method-for-sacropelvic-fixation-in-adult-spinal-deformity
#10
Jamal N Shillingford, Joseph L Laratta, Lee A Tan, Nana O Sarpong, James D Lin, Charla R Fischer, Ronald A Lehman, Yongjung J Kim, Lawrence G Lenke
BACKGROUND: Spinopelvic fixation is an integral part of achieving solid fusion across the lumbosacral junction, especially in deformity procedures requiring substantial correction or long-segment constructs. Traditional S2-alar-iliac (S2AI) screw-placement techniques utilize fluoroscopy, increasing operative time and radiation exposure to the patient and surgeon. We describe a novel free-hand technique for S2AI screw placement in patients with adult spinal deformity. METHODS: We reviewed the records of 45 consecutive patients who underwent spinopelvic fixation performed with use of S2AI screws by the senior surgeon and various fellows or residents over a 12-month period (2015 to 2016)...
February 21, 2018: Journal of Bone and Joint Surgery. American Volume
https://www.readbyqxmd.com/read/29413734/accuracy-of-s2-alar-iliac-screw-placement-under-robotic-guidance
#11
Joseph L Laratta, Jamal N Shillingford, Joseph M Lombardi, Rami G Alrabaa, Barlas Benkli, Charla Fischer, Lawrence G Lenke, Ronald A Lehman
STUDY DESIGN: Case series. OBJECTIVES: To determine the safety and feasibility of S2 alar-iliac (S2AI) screw placement under robotic guidance. SUMMARY OF BACKGROUND DATA: Similar to standard iliac fixation, S2AI screws aid in achieving fixation across the sacropelvic junction and decreasing S1 screw strain. Fortunately, the S2AI technique minimizes prominent instrumentation and the need for offset connectors to the fusion construct. Herein, we present an analysis of the largest series of robotic-guided S2AI screws in the literature without any significant author conflicts of interest with the robotics industry...
March 2018: Spine Deformity
https://www.readbyqxmd.com/read/29395522/reduced-pathogenicity-of-fructose-1-6-bisphosphatase-deficient-leishmania-donovani-and-its-use-as-an-attenuated-strain-to-induce-protective-immunogenicity
#12
Savita Saini, Ayan Kumar Ghosh, Sushmita Das, Ruby Singh, Kumar Abhishek, Sudha Verma, Ajay Kumar, Abhishek Mandal, Bidyut Purkait, Kislay Kumar Sinha, Pradeep Das
Currently, there is no approved vaccine for visceral leishmaniasis (VL) caused by L. donovani. The ability to manipulate Leishmania genome by eliminating or introducing genes necessary for parasites' survival considered as the powerful strategy to generate the live attenuated vaccine. In the present study fructose-1,6-bisphosphatase (LdFBPase) gene deleted L. donovani (Δfbpase) was generated using homologous gene replacement strategy. Though LdFBPase gene deletion (Δfbpase) does not affect the growth of parasite in the promastigote form but axenic amastigotes display a marked reduction in their capacity to multiply in vitro inside macrophages and in vivo in Balb/c mice...
February 21, 2018: Vaccine
https://www.readbyqxmd.com/read/29359951/spinal-manipulative-therapies-in-visceral-conditions
#13
Barclay W Bakkum, Gregory D Cramer
No abstract text is available yet for this article.
February 2018: Journal of Alternative and Complementary Medicine: Research on Paradigm, Practice, and Policy
https://www.readbyqxmd.com/read/29349762/advances-in-endocrine-therapy-for-postmenopausal-metastatic-breast-cancer
#14
Lisa E Flaum, William J Gradishar
A majority of breast cancers are hormone receptor (HR) positive and are responsive to various types of hormone manipulation. Endocrine therapy is the preferred first-line therapy for patients with advanced estrogen receptor (ER) positive, HER2-negative breast cancer who do not have symptomatic visceral disease. Endocrine therapy is often continued in the second- and third-line setting, with chemotherapy deferred until tumor becomes endocrine therapy refractory and/or a visceral crisis in imminent. Therapeutic options vary based on clinical presentation and include single-agent therapies such as tamoxifen, aromatase inhibitors and fulvestrant, and combination therapies options...
2018: Cancer Treatment and Research
https://www.readbyqxmd.com/read/29118214/central-network-dynamics-regulating-visceral-and-humoral-functions
#15
REVIEW
Rita J Valentino, Patrice Guyenet, Xun Helen Hou, Melissa Herman
The brain processes information from the periphery and regulates visceral and immune activity to maintain internal homeostasis, optimally respond to a dynamic external environment, and integrate these functions with ongoing behavior. In addition to its relevance for survival, this integration underlies pathology as evidenced by diseases exhibiting comorbid visceral and psychiatric symptoms. Advances in neuroanatomical mapping, genetically specific neuronal manipulation, and neural network recording are overcoming the challenges of dissecting complex circuits that underlie this integration and deciphering their function...
November 8, 2017: Journal of Neuroscience: the Official Journal of the Society for Neuroscience
https://www.readbyqxmd.com/read/29057349/clinical-evaluation-of-microendoscopy-assisted-extreme-lateral-interbody-fusion
#16
Tomohide Segawa, Hirohiko Inanami, Hisashi Koga
BACKGROUND: Extreme lateral interbody fusion (XLIF) has rapidly become a popular operative procedure for lumbar canal stenosis and scoliosis. This approach provides direct access to the lateral aspect of the disc with minimal disruption of spinal structures, including ligaments and muscles. However, it involves risk of injuries to the lumbar nerve plexus, segmental artery and intestinal tract because of the limited surgical field. This study aimed to clarify the benefit of using a microendoscope to prevent these injuries...
September 2017: Journal of Spine Surgery (Hong Kong)
https://www.readbyqxmd.com/read/28969770/a-ready-to-use-duplex-qpcr-to-detect-leishmania-infantum-dna-in-naturally-infected-dogs
#17
Rita de Cássia Pontello Rampazzo, Manuela da Silva Solcà, Liliane Celestino Sales Santos, Lais de Novaes Pereira, José Carlos Oliveira Guedes, Patrícia Sampaio Tavares Veras, Deborah Bittencourt Mothé Fraga, Marco Aurélio Krieger, Alexandre Dias Tavares Costa
Canine visceral leishmaniasis (CVL) is a systemic disease caused by Leishmania infantum. A precise CVL diagnosis would allow for a faster and more specific treatment. Quantitative PCR (qPCR) is a sensitive and specific technique that can diagnose CVL and also monitor parasite load in the animal during the course of the infection or treatment. The aim of this study was to develop a ready-to-use (gelified and freezer-free) duplex qPCR for the identification of infected animals. We combined a new qPCR protocol that detects the canine 18S rRNA gene with an existing protocol for L...
November 15, 2017: Veterinary Parasitology
https://www.readbyqxmd.com/read/28884145/sensory-experience-shapes-the-integration-of-adult-born-neurons-into-the-olfactory-bulb
#18
Elizabeth Hanson, Jessica Swanson, Benjamin R Arenkiel
Olfaction is an ancient sensory modality which is heavily involved in viscerally-important tasks like finding food and identifying mates. Olfactory processing involves interpreting stimuli from a non-continuous odor space, and translating them into an organized pattern of neuronal activity in the olfactory bulb. Additionally, olfactory processing is rapidly modulated by behavioral states and vice versa. This implies strong bidirectional neuromodulation between the olfactory bulb and other brain regions that include the cortex, hippocampus, and basal forebrain...
August 2017: Journal of Nature and Science
https://www.readbyqxmd.com/read/28874926/craniosacral-therapy-and-visceral-manipulation-a-new-treatment-intervention-for-concussion-recovery
#19
Gail Wetzler, Melinda Roland, Sally Fryer-Dietz, Dee Dettmann-Ahern
Background: Military service members and veterans face health issues related to traumatic brain injury (TBI), especially during combat, use of heavy equipment, and exposures to environmental hazards and explosives. There were 400,000 TBIs reported in deployed U.S. troops in 2012. Athletes are also subject to TBI. Studies have indicated that some manual therapies could be helpful for treating patients who have post-concussive syndrome. Objective: This case series report describes the effects of CranioSacral Therapy (CST), Visceral Manipulation (VM), and Neural Manipulation (NM) modalities for treating patients who have post-concussion syndrome...
August 1, 2017: Medical Acupuncture
https://www.readbyqxmd.com/read/28844209/the-indeterminable-resilience-of-the-fascial-system
#20
Bruno Bordoni, Fabiola Marelli, Bruno Morabito, Beatrice Sacconi
The most recent information on fascial tissue indicates that there are not fascial layers, but polyhedral microvacuoles of connective tissue, which connect the body systems and, by hosting specialized cells, permit several functions, such as motor, nervous, vascular and visceral. These microvacuoles (a repetition of polyhedral units of connective fibrils) under internal or external tension change shape and can manage the movement variations, regulating different body functions and ensuring the maintenance of efficiency of the body systems...
September 2017: Journal of Integrative Medicine
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