keyword
MENU ▼
Read by QxMD icon Read
search

ards rat

keyword
https://www.readbyqxmd.com/read/29904430/expression-profile-of-micrornas-following-bone-marrow-derived-mesenchymal-stem-cell-treatment-in-lipopolysaccharide-induced-acute-lung-injury
#1
Joonhong Park, Sikyoung Jeong, Kicheol Park, Keumjin Yang, Soyoung Shin
Immunomodulatory or immunosuppressive properties of bone marrow-derived mesenchymal stem cells (BM-MSCs) facilitate the treatment of acute respiratory distress syndrome and acute lung injury (ALI). Dysregulated miRNA (miRNA or miR) expression associated with the effects of BM-MSCs was assessed in a rat model of lipopolysaccharide (LPS)-induced ALI. The present study performed biochemical tests to assess five analytes, including alanine aminotransferase (ALT), aspartate aminotransferase (AST), lactate, blood urea nitrogen (BUN), and creatinine (CREA)...
June 2018: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/29902384/abrupt-deflation-after-sustained-inflation-causes-lung-injury
#2
Bhushan H Katira, Doreen Engelberts, Gail Otulakowski, Regan E Giesinger, Takeshi Yoshida, Martin Post, Wolfgang M Kuebler, Kim A Connelly, Brian P Kavanagh
RATIONALE: Ventilator management in ARDS focuses on 'setting' parameters and not on ventilator disconnection. OBJECTIVE: To determine if abrupt deflation after sustained inflation causes lung injury. METHODS: Male Sprague-Dawley rats were ventilated (6 mL·kg-1) and randomized to Control (n=6, PEEP 3 cmH2O; 100 min) or Intervention (n=6, PEEP 3 to 11 cmH2O over 70 min; abrupt deflation to zero PEEP; ventilation for 30 mins). Lung function and injury was assessed, scanning electron microscopy performed, and microvascular leak timed by Evans Blue dye (n=4/group at 0, 2, 5, 10 & 20 min post deflation)...
June 14, 2018: American Journal of Respiratory and Critical Care Medicine
https://www.readbyqxmd.com/read/29883714/exosomes-derived-from-endothelial-progenitor-cells-ameliorate-acute-lung-injury-by-transferring-mir-126
#3
Xu Wu, Zilong Liu, Lijuan Hu, Wenyu Gu, Lei Zhu
Endothelial progenitor cell (EPC) has potential to attenuate pulmonary inflammation and injury. As a pivotal paracrine entity of stem cells, whether EPC-derived exosomes (EPC-Exos) contribute to acute lung injury (ALI) remains unknown. Exosomes were purified from conditional medium of EPCs, and then characterized by electron micrograph and immunoblotting. A model of ALI was induced by lipopolysaccharide (LPS) and then rats were transplanted with EPC-Exos. The underlying mechanisms of action of EPC-Exos were examined in vitro endothelial functional assays including the TEER, proliferation (CKK-8), angiogenesis and migration...
June 5, 2018: Experimental Cell Research
https://www.readbyqxmd.com/read/29850515/candidate-genes-as-biomarkers-in-lipopolysaccharide-induced-acute-respiratory-distress-syndrome-based-on-mrna-expression-profile-by-next-generation-rna-seq-analysis
#4
Qi-Quan Wan, Di Wu, Qi-Fa Ye
Up until now, the regulation mechanism at the level of gene during lipopolysaccharide- (LPS-) induced acute respiratory distress syndrome (ARDS) remains unclear. The discovery of differentially expressed genes (DEGs) between LPS-induced ARDS rats and normal rats by next-generation RNA sequencing analysis is of particular interest for the current study. These DEGs may help clinical diagnosis of ARDS and facilitate the selection of the optimal treatment strategy. Randomly, 20 rats were equally divided into 2 groups, the control group and the LPS group...
2018: BioMed Research International
https://www.readbyqxmd.com/read/29771610/tracheal-acid-or-surfactant-instillation-raises-alveolar-surface-tension
#5
Tam L Nguyen, Carrie E Perlman
Whether alveolar liquid surface tension, T, is elevated in the acute respiratory distress syndrome (ARDS) has not been demonstrated in situ in the lungs. Neither is it known how exogenous surfactant, which has failed to treat ARDS, affects in situ T. We aim to determine T in an acid-aspiration ARDS model before and after exogenous surfactant administration. In isolated rat lungs, we combine servo-nulling pressure measurement and confocal microscopy to determine alveolar liquid T according to the Laplace relation...
May 17, 2018: Journal of Applied Physiology
https://www.readbyqxmd.com/read/29764547/-comparison-of-lung-protection-for-hydrochloric-acid-or-oleic-acid-induced-rat-acute-respiratory-distress-syndrome-models-pretreated-with-penehyclidine
#6
Yumei Cheng, Zhou Yang, Feng Shen, Bo Liu, Yahui Wang, Yanqi Wu, Ling Yao, Yuqing Liu
OBJECTIVE: To compare the lung protection roles of intraperitoneal pre-injection with penehyclidine for two kinds of rat models with pulmonary and extrapulmonary acute respiratory distress syndrome (ARDSp and ARDSexp). METHODS: Forty healthy adult Sprague-Dawley (SD) rats were randomly divided into five groups (each n = 8): the rats in sham group received only tracheotomy; the ARDS rat models were reproduced by intratracheal inhalation of 0.1 mol/L hydrochloric acid (HCl) 2 mL/kg to simulate ARDSexp (HCl group) and 0...
May 2018: Zhonghua Wei Zhong Bing Ji Jiu Yi Xue
https://www.readbyqxmd.com/read/29762415/distinct-biological-effects-of-time-controlled-adaptive-ventilation-in-pulmonary-and-extrapulmonary-acute-respiratory-distress-syndrome-one-small-step-for-rats-one-giant-leap-for-humans
#7
Matthieu Jabaudon, Raiko Blondonnet, Jean-Michel Constantin
No abstract text is available yet for this article.
June 2018: Critical Care Medicine
https://www.readbyqxmd.com/read/29700291/protectin-dx-increases-alveolar-fluid-clearance-in-rats-with-lipopolysaccharide-induced-acute-lung-injury
#8
Xiao-Jun Zhuo, Yu Hao, Fei Cao, Song-Fan Yan, Hui Li, Qian Wang, Bi-Huan Cheng, Bin-Yu Ying, Fang Gao Smith, Sheng-Wei Jin
Acute respiratory distress syndrome is a life-threatening critical syndrome resulting largely from the accumulation of and the inability to clear pulmonary edema. Protectin DX, an endogenously produced lipid mediator, is believed to exert anti-inflammatory and pro-resolution effects. Protectin DX (5 µg/kg) was injected i.v. 8 h after LPS (14 mg/kg) administration, and alveolar fluid clearance was measured in live rats (n = 8). In primary rat ATII epithelial cells, protectin DX (3.605 × 10-3  mg/l) was added to the culture medium with LPS for 6 h...
April 27, 2018: Experimental & Molecular Medicine
https://www.readbyqxmd.com/read/29670906/desflurane-attenuates-ventilator-induced-lung-injury-in-rats-with-acute-respiratory-distress-syndrome
#9
Xue Lin, Ying-Nan Ju, Wei Gao, Dong-Mei Li, Chang-Chun Guo
Ventilator-induced lung injury aggravates the existing lung injury. This study investigated the effect of desflurane on VILI in a rat model of acute respiratory distress syndrome. Forty-eight rats were randomized into a sham (S) group, control (C) group, lipopolysaccharide/ventilation (LV) group, lipopolysaccharide/ventilation/desflurane (LVD) group, or lipopolysaccharide/low ventilation with and without desflurane (LLV and LLVD) groups. Rats in the S group received anesthesia only. Rats in the LV and LVD groups received lipopolysaccharide and were ventilated with a high tidal volume...
2018: BioMed Research International
https://www.readbyqxmd.com/read/29670537/impact-of-different-tidal-volume-levels-at-low-mechanical-power-on-ventilator-induced-lung-injury-in-rats
#10
Lillian Moraes, Pedro L Silva, Alessandra Thompson, Cintia L Santos, Raquel S Santos, Marcos V S Fernandes, Marcelo M Morales, Vanessa Martins, Vera L Capelozzi, Marcelo G de Abreu, Paolo Pelosi, Patricia R M Rocco
Tidal volume (VT ) has been considered the main determinant of ventilator-induced lung injury (VILI). Recently, experimental studies have suggested that mechanical power transferred from the ventilator to the lungs is the promoter of VILI. We hypothesized that, as long as mechanical power is kept below a safe threshold, high VT should not be injurious. The present study aimed to investigate the impact of different VT levels and respiratory rates (RR) on lung function, diffuse alveolar damage (DAD), alveolar ultrastructure, and expression of genes related to inflammation [interleukin (IL)-6], alveolar stretch (amphiregulin), epithelial [club cell secretory protein (CC)16] and endothelial [intercellular adhesion molecule (ICAM)-1] cell injury, and extracellular matrix damage [syndecan-1, decorin, and metalloproteinase (MMP)-9] in experimental acute respiratory distress syndrome (ARDS) under low-power mechanical ventilation...
2018: Frontiers in Physiology
https://www.readbyqxmd.com/read/29650044/human-amnion-derived-mesenchymal-stem-cells-alleviate-lung-injury-induced-by-white-smoke-inhalation-in-rats
#11
Pei Cui, Haiming Xin, Yongming Yao, Shichu Xiao, Feng Zhu, Zhenyu Gong, Zhiping Tang, Qiu Zhan, Wei Qin, Yanhua Lai, Xiaohui Li, Yalin Tong, Zhaofan Xia
BACKGROUND: White smoke inhalation (WSI) is an uncommon but potentially deadly cause of acute lung injury and acute respiratory distress syndrome for which no effective pharmaceutical treatment has been developed. This study aimed to determine the protective effects of human amnion-derived mesenchymal stem cells (hAMSCs) against WSI-induced lung injury in rats. METHODS: hAMSCs were injected into rats via the tail vein 4 h after WSI. At 1, 3, 7, 14, and 28 days after cell injection, hAMSCs labeled with PKH26 in lung, heart, liver, and kidney tissues were observed by fluorescence microscopy...
April 12, 2018: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29606872/porous-se-sio-2-nanocomposites-protect-the-femoral-head-from-methylprednisolone-induced-osteonecrosis
#12
Guoying Deng, Chenyun Dai, Jinyuan Chen, Anqi Ji, Jingpeng Zhao, Yue Zhai, Yingjie Kang, Xijian Liu, Yin Wang, Qiugen Wang
Background: Methylprednisolone (MPS) is an important drug used in therapy of many diseases. However, osteonecrosis of the femoral head is a serious damage in the MPS treatment. Thus, it is imperative to develop new drugs to prevent the serious side effect of MPS. Methods: The potential interferences Se@SiO2 nanocomposites may have to the therapeutic effect of methylprednisolone (MPS) were evaluated by classical therapeutic effect index of acute respiratory distress syndrome (ARDS), such as wet-to-dry weight ratio, inflammatory factors IL-1β and TNF-α...
2018: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/29565500/expression-of-nrf2-keap1-are-signal-pathway-in-traumatic-lung-injury-and-functional-study
#13
L-Y Hu, J-B Cui, X-M Xu, Z-H Huang, H-T Jiao
OBJECTIVE: Traumatic lung injury (TLI) can cause inflammation and oxidative stress, or even leads to acute respiratory distress syndrome (ARDS) and death. Nuclear factor erythroid-2 related factor 2 (Nrf2)-Kelch-like ECH-associated protein 1 (Keap1)-antioxidant response element (ARE) signal pathway participates in disease occurrence and progression via regulating inflammatory and oxidative stress response, but with its expression and functional roles in TLI largely unknown. MATERIALS AND METHODS: Wistar rats were randomly divided into control group, TLI group by crushing method, and Nrf2 activation group which received Nrf2 specific agonist sulforaphane 30 min before TLI treatment...
March 2018: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/29554114/synthesis-of-phosphatidylcholine-in-rats-with-oleic-acid-induced-pulmonary-edema-and-effect-of-exogenous-pulmonary-surfactant-on-its-de-novo-synthesis
#14
Xiwen Gao, Peiyu Qian, Dong Cen, Weijun Hong, Qing Peng, Min Xue
In mammals, oleic acid (OA) induces pulmonary edema (PE), which can initiate acute lung injury (ALI) and lead to acute respiratory distress syndrome (ARDS). Pulmonary surfactant (PS) plays a key role in a broad range of treatments for ARDS. The aim of the present investigation was to assess changes in the synthesis of phosphatidylcholine (PC) from choline and determine the effect of exogenous PS on its de novo synthesis in rats with OA-induced PE. Experimental rats were randomized into three groups, including a control group, OA-induced PE group, and OA-induced group treated with exogenous PS (OA-PS)...
2018: PloS One
https://www.readbyqxmd.com/read/29545858/effect-of-tlr4-myd88-signaling-pathway-on-sepsis-associated-acute-respiratory-distress-syndrome-in-rats-via-regulation-of-macrophage-activation-and-inflammatory-response
#15
Shujun Zhou, Gui Wang, Wenbin Zhang
The present study aimed to investigate the effects of the Toll-like receptor (TLR)4/myeloid differentiation primary response (MyD)88 signaling pathway on sepsis-associated acute respiratory distress syndrome (ARDS) in rats, and the involvement of macrophage activation and the inflammatory response. A total of 36 specific pathogen-free male Sprague-Dawley rats were selected to establish the rat model of sepsis-associated ARDS using cecal ligation and puncture (CLP). Rats were assigned into the Ab (anti-TLR4 monoclonal antibody)-CLP, CLP and Sham groups...
April 2018: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/29522858/glucocorticoid-receptor-gene-expression-in-a-clp-induced-ards-like-rat-model-treated-with-dexamethasone-and-metyrapone
#16
Roseli Soncini, Joseana Vieira, Ana Carolina Ramos Lopes, Silvia Graciela Ruginsk, Erika K Incerpi, Angel Roberto Barchuk
Glucocorticoids (GCs) are used for acute respiratory distress syndrome (ARDS) to improve or prevent lung injury. The mechanisms underlying the effects of GCs involve inadequate GC-receptor (GR)-mediated downregulation of pro-inflammatory factors despite elevated levels of cortisol. Within this context, knowledge of the transcriptional pattern of the GR gene in response to variations in physiological parameters may shed light on this issue. We addressed this problem by measuring plasmatic corticosterone (CCT) levels and assessing GR expression at transcript and protein levels in rats with caecal ligation and puncture (CLP)-induced ARDS-like syndrome treated with dexamethasone and metyrapone...
March 6, 2018: Molecular and Cellular Endocrinology
https://www.readbyqxmd.com/read/29519276/-effects-of-acute-respiratory-distress-syndrome-induced-by-endotoxin-on-the-right-ventricular-function-in-rats
#17
Shaolei Ma, Yujie Wang, Xiangrong Zuo, Jing Yao, Quan Cao
OBJECTIVE: To explore the effect of acute respiratory distress syndrome (ARDS) induced by endotoxin on the right ventricular function in rats. METHODS: Sixty male Sprague-Dawley (SD) rats were randomly divided into normal saline (NS) control group and lipopolysaccharide (LPS) model group with 30 rats in each group. The rat model of ARDS was reproduced by intratracheal instillation of LPS 10 mg/kg after tracheotomy, and the rats in NS control group was intratracheally given the same volume of NS instead of LPS...
March 2018: Zhonghua Wei Zhong Bing Ji Jiu Yi Xue
https://www.readbyqxmd.com/read/29495169/-the-comparison-of-heparan-sulfate-and-its-fragments-on-the-protection-against-extracellular-histones-during-the-pathogenesis-of-acute-respiratory-distress-syndrome
#18
Y L Zhang, L Guan, Y M Zheng, Z M Zhao, L J Mao, S Q Li, J Y Zhao
Objective: In order to explore the role of heparan sulfate (HS) during the pathogenesis of acute respiratory distress syndrome (ARDS) , the protective effect of HS and its fragments against extracellular histones was compared. Methods: Calf thymus histones (CTH) were injected via femoral vein to induce ARDS in rats. HS, HS fragments or saline was intraperitoneally injected (10mg/kg, Q6h, 24h) to test the protective effect against CTH. The ratio of wet/dry lung weight, protein content in bronchoalveolar lavage fluid (BALF) , total leukocyte and neutrophil count in BALF were measured...
January 20, 2018: Chinese Journal of Industrial Hygiene and Occupational Diseases
https://www.readbyqxmd.com/read/29489470/biologic-impact-of-mechanical-power-at-high-and-low-tidal-volumes-in-experimental-mild-acute-respiratory-distress-syndrome
#19
Raquel S Santos, Ligia de A Maia, Milena V Oliveira, Cíntia L Santos, Lillian Moraes, Eliete F Pinto, Cynthia Dos S Samary, Joana A Machado, Anna Carolinna Carvalho, Marcos Vinícius de S Fernandes, Vanessa Martins, Vera L Capelozzi, Marcelo M Morales, Thea Koch, Marcelo Gama de Abreu, Paolo Pelosi, Pedro L Silva, Patricia R M Rocco
BACKGROUND: The authors hypothesized that low tidal volume (VT) would minimize ventilator-induced lung injury regardless of the degree of mechanical power. The authors investigated the impact of power, obtained by different combinations of VT and respiratory rate (RR), on ventilator-induced lung injury in experimental mild acute respiratory distress syndrome (ARDS). METHODS: Forty Wistar rats received Escherichia coli lipopolysaccharide intratracheally. After 24 h, 32 rats were randomly assigned to be mechanically ventilated (2 h) with a combination of different VT (6 ml/kg and 11 ml/kg) and RR that resulted in low and high power...
February 28, 2018: Anesthesiology
https://www.readbyqxmd.com/read/29485489/biological-response-to-time-controlled-adaptive-ventilation-depends-on-acute-respiratory-distress-syndrome-etiology
#20
Pedro Leme Silva, Fernanda Ferreira Cruz, Cynthia Dos Santos Samary, Lillian Moraes, Raquel Ferreira de Magalhães, Marcos Vinicius de S Fernandes, Rebeca Bose, Vitor B Pelegati, Hernandes F Carvalho, Vera Luiza Capelozzi, Joshua Satalin, Louis Gatto, Penny Andrews, Nader Habashi, Gary Nieman, Patricia R M Rocco
OBJECTIVES: To compare a time-controlled adaptive ventilation strategy, set in airway pressure release ventilation mode, versus a protective mechanical ventilation strategy in pulmonary and extrapulmonary acute respiratory distress syndrome with similar mechanical impairment. DESIGN: Animal study. SETTING: Laboratory investigation. SUBJECTS: Forty-two Wistar rats. INTERVENTIONS: Pulmonary acute respiratory distress syndrome and extrapulmonary acute respiratory distress syndrome were induced by instillation of Escherichia coli lipopolysaccharide intratracheally or intraperitoneally, respectively...
February 26, 2018: Critical Care Medicine
keyword
keyword
85128
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"