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https://www.readbyqxmd.com/read/27887604/variable-ventilation-improves-pulmonary-function-and-reduces-lung-damage-without-increasing-bacterial-translocation-in-a-rat-model-of-experimental-pneumonia
#1
Raquel F de Magalhães, Cynthia S Samary, Raquel S Santos, Milena V de Oliveira, Nazareth N Rocha, Cintia L Santos, Jamil Kitoko, Carlos A M Silva, Caroline L Hildebrandt, Cassiano F Goncalves-de-Albuquerque, Adriana R Silva, Hugo C Faria-Neto, Vanessa Martins, Vera L Capelozzi, Robert Huhle, Marcelo M Morales, Priscilla Olsen, Paolo Pelosi, Marcelo Gama de Abreu, Patricia R M Rocco, Pedro L Silva
BACKGROUND: Variable ventilation has been shown to improve pulmonary function and reduce lung damage in different models of acute respiratory distress syndrome. Nevertheless, variable ventilation has not been tested during pneumonia. Theoretically, periodic increases in tidal volume (VT) and airway pressures might worsen the impairment of alveolar barrier function usually seen in pneumonia and could increase bacterial translocation into the bloodstream. We investigated the impact of variable ventilation on lung function and histologic damage, as well as markers of lung inflammation, epithelial and endothelial cell damage, and alveolar stress, and bacterial translocation in experimental pneumonia...
November 25, 2016: Respiratory Research
https://www.readbyqxmd.com/read/27861182/cryopreserved-xeno-free-human-umbilical-cord-mesenchymal-stromal-cells-reduce-lung-injury-severity-and-bacterial-burden-in-rodent-escherichia-coli-induced-acute-respiratory-distress-syndrome
#2
Gerard F Curley, Mirjana Jerkic, Steve Dixon, Grace Hogan, Claire Masterson, Daniel O'Toole, James Devaney, John G Laffey
OBJECTIVE: Although mesenchymal stem/stromal cells represent a promising therapeutic strategy for acute respiratory distress syndrome, clinical translation faces challenges, including scarcity of bone marrow donors, and reliance on bovine serum during mesenchymal stem/stromal cell proliferation. We wished to compare mesenchymal stem/stromal cells from human umbilical cord, grown in xeno-free conditions, with mesenchymal stem/stromal cells from human bone marrow, in a rat model of Escherichia coli pneumonia...
November 16, 2016: Critical Care Medicine
https://www.readbyqxmd.com/read/27830717/effect-of-heme-oxygenase-1-on-mitofusin-1-protein-in-lps-induced-ali-ards-in-rats
#3
Jianbo Yu, Ying Wang, Zhen Li, Shuan Dong, Dan Wang, Lirong Gong, Jia Shi, Yuan Zhang, Daquan Liu, Rui Mu
Acute lung injury (ALI)/acute respiratory distress syndrome (ARDS) is a common and important oxidative stress in the lung. Mitochondrial fusion responds to the normal morphology and function of cells and is finely regulated by mitochondrial fusion proteins, such as mitofusin-1 protein (Mfn1), mitofusin-2 protein (Mfn2) and optical atrophy 1 (OPA1). Additionally, Mfn1 has been identified as the most important protein in mitochondrial fusion. Heme oxygenase-1 (HO-1) is a stress-inducible protein that plays a critical role in protecting against oxidative stress...
November 10, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27812430/synthetic-lung-surfactants-containing-sp-b-and-sp-c-peptides-plus-novel-phospholipase-resistant-lipids-or-glycerophospholipids
#4
Robert H Notter, Rohun Gupta, Adrian L Schwan, Zhengdong Wang, Mohanad Gh Shkoor, Frans J Walther
BACKGROUND: This study examines the biophysical and preclinical pulmonary activity of synthetic lung surfactants containing novel phospholipase-resistant phosphonolipids or synthetic glycerophospholipids combined with Super Mini-B (S-MB) DATK and/or SP-Css ion-lock 1 peptides that replicate the functional biophysics of surfactant proteins (SP)-B and SP-C. Phospholipase-resistant phosphonolipids used in synthetic surfactants are DEPN-8 and PG-1, molecular analogs of dipalmitoyl phosphatidylcholine (DPPC) and palmitoyl-oleoyl phosphatidylglycerol (POPG), while glycerophospholipids used are active lipid components of native surfactant (DPPC:POPC:POPG 5:3:2 by weight)...
2016: PeerJ
https://www.readbyqxmd.com/read/27779587/the-renin-inhibitor-aliskiren-protects-rat-lungs-from-the-histopathological-effects-of-fat-embolism
#5
Amanda N Fletcher, Agostino Molteni, Rakesh Ponnapureddy, Chirag Patel, Mark Pluym, Alan M Poisner
BACKGROUND: Fat embolism (FE) and the consequent fat embolism syndrome (FES) occurring after trauma or surgery can lead to serious pulmonary injury, including ARDS and death. Current treatment of FES is limited to supportive therapy. We have shown in a rat model that the renin angiotensin system (RAS) plays a significant role in the pathophysiology of FE since drugs interfering with the RAS, captopril and losartan, reduce the histopathologic pulmonary damage. The purpose of the current study was to determine if inhibition of renin by aliskiren, an FDA-approved drug for treating hypertension, would produce effective protection in the same model...
October 25, 2016: Journal of Trauma and Acute Care Surgery
https://www.readbyqxmd.com/read/27778032/activation-of-necroptosis-in-a-rat-model-of-acute-respiratory-distress-syndrome-induced-by-oleic-acid
#6
Long Pan, Dun-Chen Yao, Yu-Zhong Yu, Bing-Jun Chen, Sheng-Jie Li, Gui-He Hu, Chang Xi, Zi-Hui Wang, Jian-Hua Li, Jie Long, Yong-Sheng Tu
The present study was aimed to investigate the role of necroptosis in the pathogenesis of acute respiratory distress syndrome (ARDS). The rat model of ARDS was induced by intravenous injection of oleic acid (OA), and observed for 4 h. The lung injury was evaluated by arterial blood gas, lung wet-dry weight ratio (W/D) and histological analyses. Simultaneously, bronchoalveolar lavage fluid (BALF) was collected for total and differential cell analysis and total protein determination. Tumor necrosis factor alpha (TNF-α) level in BALF was determined with a rat TNF-α ELISA kit...
October 25, 2016: Sheng Li Xue Bao: [Acta Physiologica Sinica]
https://www.readbyqxmd.com/read/27765761/nur77-attenuates-endothelin-1-expression-via-downregulation-of-nf-%C3%AE%C2%BAb-and-p38-mapk-in-a549-cells-and-in-an-ards-rat-model
#7
Yujie Jiang, Yi Zeng, Xia Huang, Yueqiu Qin, Weigui Luo, Shulin Xiang, Suren R Sooranna, Liao Pinhu
Acute respiratory distress syndrome (ARDS) is characterized by inflammatory injury to the alveolar and capillary barriers that results in impaired gas exchange and severe acute respiratory failure. Nuclear orphan receptor Nur77 has emerged as a regulator of gene expression in inflammation, and its role in the pathogenesis of ARDS is not clear. The objective of this study is to investigate the potential role of Nur77 and its underlying mechanism in the regulation of endothelin-1 (ET-1) expression in lipopolysaccharide (LPS)-induced A549 cells and an ARDS rat model...
December 1, 2016: American Journal of Physiology. Lung Cellular and Molecular Physiology
https://www.readbyqxmd.com/read/27701117/role-of-acid-sphingomyelinase-and-il-6-as-mediators-of-endotoxin-induced-pulmonary-vascular-dysfunction
#8
Rachele Pandolfi, Bianca Barreira, Enrique Moreno, Victor Lara-Acedo, Daniel Morales-Cano, Andrea Martínez-Ramas, Beatriz de Olaiz Navarro, Raquel Herrero, José Ángel Lorente, Ángel Cogolludo, Francisco Pérez-Vizcaíno, Laura Moreno
BACKGROUND: Pulmonary hypertension (PH) is frequently observed in patients with acute respiratory distress syndrome (ARDS) and it is associated with an increased risk of mortality. Both acid sphingomyelinase (aSMase) activity and interleukin 6 (IL-6) levels are increased in patients with sepsis and correlate with worst outcomes, but their role in pulmonary vascular dysfunction pathogenesis has not yet been elucidated. Therefore, the aim of this study was to determine the potential contribution of aSMase and IL-6 in the pulmonary vascular dysfunction induced by lipopolysaccharide (LPS)...
July 27, 2016: Thorax
https://www.readbyqxmd.com/read/27664487/budesonide-attenuates-ventilator-induced-lung-injury-in-a-rat-model-of-inflammatory-acute-respiratory-distress-syndrome
#9
Wei Gao, Ying-Nan Ju
BACKGROUND AND AIMS: Patients with acute respiratory distress syndrome (ARDS) are particularly susceptible to ventilator-induced lung injury (VILI). This study investigated the effect of budesonide on VILI in a rat model of inflammatory ARDS. METHODS: Forty eight rats were randomized into three groups (n = 16 each): sham group (S), endotoxin/ventilation group (LV), endotoxin/ventilation/budesonide group (LVB). Rats in the S group received anesthesia only. Rats in the LV and LVB groups received endotoxin to simulate ARDS and were mechanically ventilated for 4 h (tidal volume 30 mL/kg)...
May 2016: Archives of Medical Research
https://www.readbyqxmd.com/read/27586277/necrostatin-1-protects-against-oleic-acid-induced-acute-respiratory-distress-syndrome-in-rats
#10
Long Pan, Dun-Chen Yao, Yu-Zhong Yu, Sheng-Jie Li, Bing-Jun Chen, Gui-He Hu, Chang Xi, Zi-Hui Wang, Hong-Yan Wang, Jian-Hua Li, Yong-Sheng Tu
Necroptosis is a recently discovered necrotic cell death which is regulated by receptor interacting protein kinase 1 (RIPK1) and RIPK3 under the stimulus of death signal and can be inhibited by necrostatin-1 (Nec-1) specifically. Therefore, the aim was to investigate the role of necroptosis in a rat model of acute respiratory distress syndrome (ARDS) induced by oleic acid (OA) and assess the effect of Nec-1 on lung injury in ARDS. Our results found that RIPK1, RIPK3 and mixed lineage kinase domain-like protein (MLKL) were abundantly expressed in rat lung tissues of OA-induced ARDS...
September 30, 2016: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/27513359/age-dependent-changes-in-the-pulmonary-renin-angiotensin-system-are-associated-with-severity-of-lung-injury-in-a-model-of-acute-lung-injury-in-rats
#11
Laura R A Schouten, Hendrik J F Helmerhorst, Gerry T M Wagenaar, Tom Haltenhof, René Lutter, Joris J T H Roelofs, Job B M van Woensel, Anton H L C van Kaam, Albert P Bos, Marcus J Schultz, Thomas Walther, Roelie M Wösten-van Asperen
OBJECTIVES: A growing body of evidence suggests that age affects the main pathophysiologic mechanisms of the acute respiratory distress syndrome. This may imply the need for developing age-tailored therapies for acute respiratory distress syndrome. However, underlying molecular mechanisms governing age-related susceptibility first need to be unraveled. In a rat model of acute lung injury, we investigated whether age affects the balance between the two key enzymes of the pulmonary renin-angiotensin system, angiotensin-converting enzyme, and angiotensin-converting enzyme 2...
December 2016: Critical Care Medicine
https://www.readbyqxmd.com/read/27499930/strong-correlation-between-lung-ultrasound-and-chest-computerized-tomography-imaging-for-the-detection-of-acute-lung-injury-acute-respiratory-distress-syndrome-in-rats
#12
Huan Ma, Daozheng Huang, Liheng Guo, Quanfu Chen, Wenzhao Zhong, Qingshan Geng, Minzhou Zhang
BACKGROUND: Lung ultrasound (LUS) is a clinical imaging technique for diagnosing acute lung injury (ALI) and acute respiratory distress syndrome (ARDS). In humans and several large animals, LUS demonstrates similar specificity and sensitivity to computerized tomography (CT) scanning. Current study evaluated the degree of agreement between LUS and CT imaging in characterizing ALI/ARDS in rats. METHODS: Thirty male Sprague-Dawley rats were imaged by LUS before randomization into three groups to receive intratracheal saline, 3 or 6 mg/kg LPS respectively (n=10)...
July 2016: Journal of Thoracic Disease
https://www.readbyqxmd.com/read/27488006/dual-hit-lipopolysaccharide-oleic-acid-combination-induced-rat-model-of-acute-lung-injury-acute-respiratory-distress-syndrome
#13
T N Hagawane, R V Gaikwad, N A Kshirsagar
BACKGROUND & OBJECTIVES: Despite advances in therapy and overall medical care, acute lung injury (ALI)/acute respiratory distress syndrome (ARDS) management remains a problem. Hence the objective of this study was to develop a rat model that mimics human ALI/ARDS. METHODS: Four groups of Wistar rats, 48 per group were treated with (i) intratracheal (IT) lipopolysaccharide (LPS) (5 mg/kg) dissolved in normal saline (NS), (ii) intravenous (iv) oleic acid (OA) (250 μl/kg) suspension in bovine serum albumin (BSA), (iii) dual hit: IT LPS (2 mg/kg) dissolved in NS and iv OA (100 μl/kg) and (iv) control group: IT NS and iv BSA...
May 2016: Indian Journal of Medical Research
https://www.readbyqxmd.com/read/27473159/the-effects-of-dexamethasone-and-l-name-on-acute-lung-injury-in-rats-with-lung-contusion
#14
Ahmet Kozan, Nermin Kilic, Hasan Alacam, Ahmet Guzel, Tolga Guvenc, Mehmet Acikgoz
The therapeutic efficiency of an anti-inflammatory agent, dexamethasone (DXM), and a nitric oxide synthase (NOS) inhibitor, Nitro-L-arginine methyl ester (L-NAME), in lung tissue injury after lung contusion was investigated. Serum levels of tumor necrosis factor-alpha (TNF-α), interleukin-10 (IL-10), YKL-40, an inflammatory peptide, inducible NOS (iNOS), and Clara cell protein 16 (CC-16) were evaluated. Immunohistochemical analyses were also performed, and the lung tissue was examined histopathologically. The study consisted of eight groups of Sprague-Dawley rats (n = 10 in each group), weighing 250-300 g: (1) control, (2) contusion, (3) control + DXM, (4) contusion + DXM, (5) control + L-NAME (6) contusion + L-NAME, (7) control + DXM + L-NAME, and (8) contusion + DXM + L-NAME...
October 2016: Inflammation
https://www.readbyqxmd.com/read/27411461/inhibition-of-c-jun-n-terminal-kinase-signaling-pathway-alleviates-lipopolysaccharide-induced-acute-respiratory-distress-syndrome-in-rats
#15
Jian-Bo Lai, Chun-Fang Qiu, Chuan-Xi Chen, Min-Ying Chen, Juan Chen, Xiang-Dong Guan, Bin Ouyang
BACKGROUND: An acute respiratory distress syndrome (ARDS) is still one of the major challenges in critically ill patients. This study aimed to investigate the effect of inhibiting c-Jun N-terminal kinase (JNK) on ARDS in a lipopolysaccharide (LPS)-induced ARDS rat model. METHODS: Thirty-six rats were randomized into three groups: control, LPS, and LPS + JNK inhibitor. Rats were sacrificed 8 h after LPS treatment. The lung edema was observed by measuring the wet-to-dry weight (W/D) ratio of the lung...
July 20, 2016: Chinese Medical Journal
https://www.readbyqxmd.com/read/27343405/activation-of-angiotensin-converting-enzyme-2-ace2-attenuates-allergic-airway-inflammation-in-rat-asthma-model
#16
Vaibhav Shrirang Dhawale, Venkateswara Rao Amara, Pinakin Arun Karpe, Vajir Malek, Deep Patel, Kulbhushan Tikoo
Angiotensin-I converting enzyme (ACE) is positively correlated to asthma, chronic obstructive pulmonary disease (COPD), acute respiratory distress syndrome (ARDS) and is highly expressed in lungs. ACE2, the counteracting enzyme of ACE, was proven to be protective in pulmonary, cardiovascular diseases. In the present study we checked the effect of ACE2 activation in animal model of asthma. Asthma was induced in male wistar rats by sensitization and challenge with ovalbumin and then treated with ACE2 activator, diminazene aceturate (DIZE) for 2weeks...
September 1, 2016: Toxicology and Applied Pharmacology
https://www.readbyqxmd.com/read/27313398/the-vascular-endothelial-growth-factors-expressing-character-of-mesenchymal-stem-cells-plays-a-positive-role-in-treatment-of-acute-lung-injury-in-vivo
#17
Yi Yang, Shuling Hu, Xiuping Xu, Jinze Li, Airan Liu, Jibin Han, Songqiao Liu, Ling Liu, Haibo Qiu
Recently, mesenchymal stem cells (MSC) have been proved to be beneficial in acute respiratory distress syndrome (ARDS). Vascular endothelial growth factor (VEGF) is an important angiogenesis factor that MSC release. However, the precise role of VEGF-expressing character of MSC in the MSC treatment for ARDS remains obscure. Here, we firstly knocked down the gene VEGF in MSC (MSC-ShVEGF) with lentiviral transduction. Then we injected the MSC-ShVEGF to rats with lipopolysaccharide-induced acute lung injury (ALI) via the tail vein...
2016: Mediators of Inflammation
https://www.readbyqxmd.com/read/27302421/angiotensin-converting-enzyme-2-prevents-lipopolysaccharide-induced-rat-acute-lung-injury-via-suppressing-the-erk1-2-and-nf-%C3%AE%C2%BAb-signaling-pathways
#18
Yingchuan Li, Zhen Zeng, Yongmei Cao, Yujing Liu, Feng Ping, Mengfan Liang, Ying Xue, Caihua Xi, Ming Zhou, Wei Jiang
Acute respiratory distress syndrome (ARDS) caused by severe sepsis remains a major challenge in intensive care medicine. ACE2 has been shown to protect against lung injury. However, the mechanisms of its protective effects on ARDS are largely unknown. Here, we report that ACE2 prevents LPS-induced ARDS by inhibiting MAPKs and NF-κB signaling pathway. Lentiviral packaged Ace2 cDNA or Ace2 shRNA was intratracheally administrated into the lungs of male SD rats. Two weeks after gene transfer, animals received LPS (7...
2016: Scientific Reports
https://www.readbyqxmd.com/read/27129877/the-hepatocyte-growth-factor-expressing-character-is-required-for-mesenchymal-stem-cells-to-protect-the-lung-injured-by-lipopolysaccharide-in-vivo
#19
Shuling Hu, Jinze Li, Xiuping Xu, Airan Liu, Hongli He, Jingyuan Xu, Qihong Chen, Songqiao Liu, Ling Liu, Haibo Qiu, Yi Yang
BACKGROUND: Acute respiratory distress syndrome (ARDS) is a life-threatening condition in critically ill patients. Recently, we have found that mesenchymal stem cells (MSC) improved the permeability of human lung microvascular endothelial cells by secreting hepatocyte growth factor (HGF) in vitro. However, the properties and functions of MSC may change under complex circumstances in vivo. Here, we sought to determine the role of the HGF-expressing character of MSC in the therapeutic effects of MSC on ARDS in vivo...
2016: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/27058048/preactivated-and-disaggregated-shape-changed-platelets-protected-against-acute-respiratory-distress-syndrome-complicated-by-sepsis-through-inflammation-suppression
#20
Yuan-Ji Day, Kuan-Hung Chen, Yi-Ling Chen, Tien-Hung Huang, Pei-Hsun Sung, Fan-Yen Lee, Chih-Hung Chen, Han-Tan Chai, Tsung-Cheng Yin, Hsin-Ju Chiang, Sheng-Ying Chung, Hsueh-Wen Chang, Hon-Kan Yip
BACKGROUND: This study tested the hypothesis that preactivated and disaggregated shape-changed platelet (PreD-SCP) therapy attenuates lung injury from acute respiratory distress syndrome (ARDS) induced by 100% oxygen inhalation and complicated by sepsis through peritoneal administration of 1.5 mg/kg lipopolysaccharide (LPS). METHODS: Adult male Sprague-Dawley rats, weighing 325-350 g, were randomized into group 1 [normal controls (NC)], group 2 [NC + PreD-SCP (3...
March 30, 2016: Shock
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