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https://www.readbyqxmd.com/read/29851454/cross-linked-fluorescent-supramolecular-nanoparticles-for-intradermal-controlled-release-of-antifungal-drug-a-therapeutic-approach-for-onychomycosis
#1
Fang Wang, Peng Yang, Jin-Sil Choi, Petar Antovski, Yazhen Zhu, Xiaobin Xu, Ting-Hao Kuo, Li-En Lin, Diane N H Kim, Pin-Cheng Huang, Haoxiang Xu, Chin-Fa Lee, Changchun Wang, Cheng-Chih Hsu, Kai Chen, Paul S Weiss, Hsian-Rong Tseng
The existing approaches to onychomycosis demonstrate limited success since the commonly used oral administration and topical cream only achieve temporary effective drug concentration at the fungal infection sites. An ideal therapeutic approach for onychomycosis should have (i) the ability to introduce anti-fungal drugs directly to the infected sites; (ii) finite intradermal sustainable release to maintain effective drug levels over prolonged time; (iii) a reporter system for monitoring maintenance of drug level; and (iv) minimum level of inflammatory responses at or around the fungal infection sites...
May 31, 2018: ACS Nano
https://www.readbyqxmd.com/read/29778469/communications-of-staphylococcus-aureus-and-non-aureus-staphylococcus-species-from-bovine-intramammary-infections-and-teat-apex-colonization
#2
Yasser S Mahmmod, Ilka Christine Klaas, Line Svennesen, Karl Pedersen, Hanne Ingmer
The role of non-aureus staphylococci (NAS) in the risk of acquisition of intramammary infections with Staphylococcus aureus is vague and still under debate. The objectives of this study were to (1) investigate the distribution patterns of NAS species from milk and teat skin in dairy herds with automatic milking systems, and (2) examine if the isolated NAS influences the expression of S. aureus virulence factors controlled by the accessory gene regulator (agr) quorum sensing system. In 8 herds, 14 to 20 cows with elevated somatic cell count were randomly selected for teat skin swabbing and aseptic quarter foremilk samples from right hind and left front quarters...
May 16, 2018: Journal of Dairy Science
https://www.readbyqxmd.com/read/29755726/-trueperella-pyogenes-and-brucella-abortus-coinfection-in-a-dog-and-a-cat-on-a-dairy-farm-in-egypt-with-recurrent-cases-of-mastitis-and-abortion
#3
Gamal Wareth, Mohamed El-Diasty, Falk Melzer, Jayaseelan Murugaiyan, Amir Abdulmawjood, Lisa D Sprague, Heinrich Neubauer
Trueperella pyogenes was isolated from a dog and a cat with a mixed infection with Brucella abortus . Both lived on a dairy cattle farm with a history of regular cases of abortion and mastitis. Identification of the bacteria was done by means of MALDI-TOF MS, loop-mediated isothermal amplification (LAMP) based on cpn 60, partial 16S rRNA sequencing, and growth on Loeffler Serum Medium. Isolation of Trueperella pyogenes on the dairy farm highlights its neglected role in reproduction failure and draws attention to its effects in the dairy industry in Egypt...
2018: Veterinary Medicine International
https://www.readbyqxmd.com/read/29740410/growth-and-extended-survival-of-escherichia-coli-o157-h7-in-soil-organic-matter
#4
Gitanjali NandaKafle, Amy A Christie, Sébastien Vilain, Volker S Brözel
Enterohaemorrhagic Escherichia coli , such as serotype O157:H7, are a leading cause of food-associated outbreaks. While the primary reservoir is associated with cattle, plant foods have been associated as sources of human infection. E. coli is able to grow in the tissue of food plants such as spinach. While fecal contamination is the primary suspect, soil has been underestimated as a potential reservoir. Persistence of bacterial populations in open systems is the product of growth, death, predation, and competition...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29738604/five-year-profile-of-candidemia-at-an-indian-trauma-center-high-rates-of-candida-auris-blood-stream-infections
#5
Purva Mathur, Fahmi Hasan, Pradeep Kumar Singh, Rajesh Malhotra, Kamini Walia, Anuradha Chowdhary
BACKGROUND: Candidemia is a potentially fatal infection with varied distribution of Candida species and their antifungal susceptibility profiles. The recent emergence of Candida auris in invasive candidiasis is a cause for concern. This study describes the profile of candidemia at an Indian tertiary care hospital and reports the emergence of Candida auris. METHODS: All patients diagnosed as having candidemia between 2012 to 2017 were studied. The isolates were identified using conventional methods, VITEK 2 and by the MALDI-TOF MS...
May 8, 2018: Mycoses
https://www.readbyqxmd.com/read/29698754/hmw-profiling-using-maldi-tof-ms-a-screening-method-for-outbreaks-of-clostridioides-difficile
#6
Lucía Ortega, Anna Ryberg, Åsa Johansson
Clostridioides difficile (CD), previously known as Clostridium difficile, is an anaerobic Gram-positive rod-shaped bacterium that causes mild to severe diarrhea mainly in hospitalized patients. The bacteria are easily spread between patients and can persist in hospital wards due to its ability to form spores. An outbreak of CD causes great sufferings for patients and is in many aspects very expensive for the health care organization. Continuously monitoring circulating CD isolates in the hospital as well as being able to detect possible spread between patients at an early phase would be of great benefit...
April 24, 2018: Anaerobe
https://www.readbyqxmd.com/read/29671790/chemical-synthesis-and-functional-analysis-of-varva-cyclotide
#7
Claudio A Álvarez, Paula A Santana, Omar Luna, Constanza Cárdenas, Fernando Albericio, María S Romero, Fanny Guzmán
Cyclotides are circular peptides found in various plant families. A cyclized backbone, together with multiple disulfide bonds, confers the peptides’ exceptional stability against protease digestion and thermal denaturation. In addition, the features of these antimicrobial molecules make them suitable for use in animal farming, such as aquaculture. Fmoc solid phase peptide synthesis on 2-chlorotrityl chlorine (CTC) resin using the “tea-bag” approach was conducted to generate the VarvA cyclotide identified previously from Viola arvensis ...
April 19, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29617332/rapid-identification-of-intact-staphylococcal-bacteriophages-using-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry
#8
Dana Štveráková, Ondrej Šedo, Martin Benešík, Zbyněk Zdráhal, Jiří Doškař, Roman Pantůček
Staphylococcus aureus is a major causative agent of infections associated with hospital environments, where antibiotic-resistant strains have emerged as a significant threat. Phage therapy could offer a safe and effective alternative to antibiotics. Phage preparations should comply with quality and safety requirements; therefore, it is important to develop efficient production control technologies. This study was conducted to develop and evaluate a rapid and reliable method for identifying staphylococcal bacteriophages, based on detecting their specific proteins using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) profiling that is among the suggested methods for meeting the regulations of pharmaceutical authorities...
April 4, 2018: Viruses
https://www.readbyqxmd.com/read/29608117/clinical-impact-of-rapid-bacterial-identification-by-maldi-tof-ms-combined-with-the-b%C3%A3%C2%AAta-lacta%C3%A2-test-on-early-antibiotic-adaptation-by-an-antimicrobial-stewardship-team-in-bloodstream-infections
#9
A Mizrahi, J Amzalag, C Couzigou, G Péan De Ponfilly, B Pilmis, A Le Monnier
BACKGROUND: Bloodstream infections (BSI) can potentially be life-threatening infections and are associated with a high crude mortality, moreover with an inappropriate first-line antibiotic therapy. Bacterial resistance is more and more frequently observed. New strategies of BSI management are urgently needed. MATERIALS AND METHODS: During an 18-months period, we prospectively evaluated the clinical impact of rapid bacterial identification by MALDI-TOF MS technology combined with an antimicrobial stewardship team (AST) intervention...
April 2, 2018: Infectious Diseases
https://www.readbyqxmd.com/read/29534106/a-new-scheme-for-strain-typing-of-methicillin-resistant-staphylococcus-aureus-on-the-basis-of-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry-by-using-machine-learning-approach
#10
Hsin-Yao Wang, Tzong-Yi Lee, Yi-Ju Tseng, Tsui-Ping Liu, Kai-Yao Huang, Yung-Ta Chang, Chun-Hsien Chen, Jang-Jih Lu
Methicillin-resistant Staphylococcus aureus (MRSA), one of the most important clinical pathogens, conducts an increasing number of morbidity and mortality in the world. Rapid and accurate strain typing of bacteria would facilitate epidemiological investigation and infection control in near real time. Matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry is a rapid and cost-effective tool for presumptive strain typing. To develop robust method for strain typing based on MALDI-TOF spectrum, machine learning (ML) is a promising algorithm for the construction of predictive model...
2018: PloS One
https://www.readbyqxmd.com/read/29451890/detection-of-bartonella-spp-in-fleas-by-maldi-tof-ms
#11
Basma El Hamzaoui, Maureen Laroche, Lionel Almeras, Jean-Michel Bérenger, Didier Raoult, Philippe Parola
BACKGROUND: Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) has recently emerged in the field of entomology as a promising method for the identification of arthropods and the detection of associated pathogens. METHODOLOGY/PRINCIPAL FINDINGS: An experimental model of Ctenocephalides felis (cat fleas) infected with Bartonella quintana and Bartonella henselae was developed to evaluate the efficacy of MALDI-TOF MS in distinguishing infected from uninfected fleas, and its ability to distinguish fleas infected with Bartonella quintana from fleas infected with Bartonella henselae...
February 2018: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/29421668/first-report-of-sporadic-cases-of-candida-auris-in-colombia
#12
Claudia M Parra-Giraldo, Sandra L Valderrama, Gloria Cortes-Fraile, Javier R Garzón, Beatriz E Ariza, Florent Morio, Melva Y Linares-Linares, Andrés Ceballos-Garzón, Alejandro de la Hoz, Catalina Hernandez, Carlos Alvarez-Moreno, Patrice Le Pape
BACKGROUND: Candida auris is a recently reported Candida species that is phenotypically similar to Candida haemulonii and related to hospital outbreaks. This organism can be misidentified as Candida haemulonii, Candida famata, Candida catenulata, or Rhodotorula glutinis by phenotypic approaches. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and DNA sequence analysis using internal transcribed spacer rDNA bar-coding provide an accurate identification...
April 2018: International Journal of Infectious Diseases: IJID
https://www.readbyqxmd.com/read/29345117/candida-auris-a-systematic-review-and-meta-analysis-of-current-updates-on-an-emerging-multidrug-resistant-pathogen
#13
John Osei Sekyere
From 2009, Candida auris has emerged as a multidrug-resistant ascomycete yeast pathogen with the capacity for easy transmission between patients and hospitals, as well as persistence on environmental surfaces. Its association with high mortalities, breakthrough and persistent candidaemia, inconsistencies in susceptibility testing results, misidentification by available commercial identification systems and treatment failure, complicates its management and detection. Within the last nine years, C. auris has been increasingly reported from far-Eastern Asia, the Middle East, Africa, Europe, South and North America with substantial fatalities and misidentification...
January 18, 2018: MicrobiologyOpen
https://www.readbyqxmd.com/read/29344802/protein-profiling-of-acanthamoeba-species-using-maldi-tof-ms-for-specific-identification-of-acanthamoeba-genotype
#14
Kirti Megha, Megha Sharma, Amit Gupta, Rakesh Sehgal, Sumeeta Khurana
Acanthamoeba spp. are ubiquitous in the environment and have the potential to cause severe infections. The different genotypes of Acanthamoeba have been shown to influence the severity of the disease and response to therapy. Characterizing Acanthamoeba spp. upto genotype can aid in infection control practices. Twenty-five Acanthamoeba isolates, characterized by 18S rDNA sequencing, were subjected to MALDI-TOF MS analysis by creating a database for the individual genotypes. The differentiating features of the various spectra were observed; the coded samples were then tested against the created database...
January 17, 2018: Parasitology Research
https://www.readbyqxmd.com/read/29285820/maldi-imaging-detects-endogenous-digoxin-in-glioblastoma-cells-infected-by-zika-virus-would-it-be-the-oncolytic-key
#15
Estela de O Lima, Tatiane M Guerreiro, Carlos Fernando O R Melo, Diogo N de Oliveira, Daisy Machado, Marcelo Lancelloti, Rodrigo R Catharino
No abstract text is available yet for this article.
March 2018: Journal of Mass Spectrometry: JMS
https://www.readbyqxmd.com/read/29224733/validated-maldi-tof-ms-method-for-anthrax-lethal-factor-provides-early-diagnosis-and-evaluation-of-therapeutics
#16
Maribel Gallegos-Candela, Anne E Boyer, Adrian R Woolfitt, Judy Brumlow, Renato C Lins, Conrad P Quinn, Alex R Hoffmaster, Gabriel Meister, John R Barr
Anthrax lethal factor (LF) is a zinc-dependent endoprotease and a critical virulence factor for Bacillus anthracis, the causative agent of anthrax. The mass spectrometry (MS) method for total-LF quantification includes three steps; 1) LF specific antibody capture/concentration, 2) LF-specific hydrolysis of a peptide substrate, and 3) detection and quantification of LF-cleaved peptides by isotope-dilution MALDI-TOF/MS. Recombinant LF spiked plasma was used for calibration and quality control (QC) materials. Specificity was 100% from analysis of serum and plasma from 383 non-infected humans, 31 rabbits, and 24 rhesus macaques...
February 15, 2018: Analytical Biochemistry
https://www.readbyqxmd.com/read/29193200/rapid-identification-of-microorganisms-from-platelet-concentrates-by-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry-after-short-term-incubation-on-liquid-medium
#17
Yasmine Chetouane, Gregory Dubourg, Pierre Gallian, Christophe Flaudrops, Jacques Chiaroni, Eric Chabrière, Didier Raoult, Laurence Camoin-Jau
BACKGROUND: Platelets (PLTs) are especially affected by the risk of bacterial contamination. Matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) is an accurate method for the routine identification of bacterial isolates in microbiology laboratories. We directly applied the MALDI-TOF method to bacterial detection in PLTs. In this study, we evaluated the sensitivity, specificity, and speed of a direct MALDI-TOF approach compared to the conventional method BACTEC. STUDY DESIGN AND METHODS: Eight bacteria associated with PLT contamination, cited by the ISBT on transfusion-transmitted infectious diseases, were spiked into PLTs for a final concentration of approximately 100 CFU/bag (n = 5 for each strain)...
March 2018: Transfusion
https://www.readbyqxmd.com/read/29076587/high-prevalence-of-candiduria-due-to-non-albicans-candida-species-among-diabetic-patients-a-matter-of-concern
#18
Alireza Esmailzadeh, Hossein Zarrinfar, AbdolMajid Fata, Tanuka Sen
BACKGROUND: Among the fungal pathogens, Candida species are the most common cause of urinary tract infection (UTI). Some predisposing factors such as diabetes mellitus, urinary retention, urinary stasis, renal transplantation, and hospitalization can increase the risk of candiduria. The aim of this cross-sectional study was to evaluate candiduria among type 2 diabetic patients and identification of the Candida isolates. METHOD: Four hundred clean-catch midstream urine specimens were obtained from patients with type 2 diabetes mellitus...
October 27, 2017: Journal of Clinical Laboratory Analysis
https://www.readbyqxmd.com/read/29055988/-association-of-hla-dq-and-ifnl4-polymorphisms-with-hepatitis-b-virus-infection-and-clearance
#19
H Chen, J H Fan, W X Chen, J J Guo, Y Huang
Objective: To confirmed the polymorphisms of HLA-DQ and IFNL4 were associated with HBV infection and clearance in a Chinese population. Methods: The Sequenom MassARRAY MALDI-TOF system was used to genotype the HLA-DQrs9275319 and IFNL4rs368234815, rs12971396, rs12979860, and rs8099917. A binary logistic regression test was conducted to estimate the relative risk of these SNPs with HBV infection and clearance. Haploview4.2 software and PHASE software (v2.0.2) were employed to analyze linkage disequilibrium (LD) and haplotype frequencies...
July 20, 2017: Zhonghua Gan Zang Bing za Zhi, Zhonghua Ganzangbing Zazhi, Chinese Journal of Hepatology
https://www.readbyqxmd.com/read/29022241/a-panel-of-protein-candidates-for-comprehensive-study-of-caprine-arthritis-encephalitis-cae-infection
#20
Rosivaldo Quirino Bezerra Júnior, Ângela Maria Xavier Eloy, João Ricardo Furtado, Raymundo Rizaldo Pinheiro, Alice Andrioli, Frederico Bruno Moreno, Marina Duarte Pinto Lobo, Ana Cristina O Monteiro-Moreira, Renato de Azevedo Moreira, Tatiana Maria Farias Pinto, Maria Fátima da Silva Teixeira
The caprine arthrite encephalite (CAE) is a disease that affects especially dairy goat. The virus shows compartmentalization features, that allows it to hide at certain times during the course of the disease, making it difficult to control. The present study was conducted to identify the major seminal plasma protein profile of goats infected by CAE and its associations with seroconversion using Western blotting. Two groups containing five males each, were used in this experiment. The first group was composed by seropositive animals and the control by seronegative confirmed by Western blotting and PCR...
January 2018: Tropical Animal Health and Production
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