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https://www.readbyqxmd.com/read/28430811/antimicrobial-resistance-in-coagulase-positive-staphylococci-isolated-from-companion-animals-in-australia-a-one-year-study
#1
Sugiyono Saputra, David Jordan, Kate A Worthing, Jacqueline M Norris, Hui S Wong, Rebecca Abraham, Darren J Trott, Sam Abraham
Methicillin-resistant coagulase-positive staphylococci (CoPS) have become increasingly recognised as opportunistic pathogens that limit therapeutic options in companion animals. The frequency of methicillin resistance amongst clinical isolates on an Australia-wide level is unknown. This study determined antimicrobial susceptibility patterns for CoPS isolated from clinical infections in companion animals (dogs, cats and horses) as part of the first nation-wide survey on antimicrobial resistance in animal pathogens in Australia for a one-year period (January 2013 to January 2014)...
2017: PloS One
https://www.readbyqxmd.com/read/28404678/antifungal-susceptibility-testing-of-aspergillus-spp-by-using-a-cci-based-maldi-tof-ms-method-appears-to-not-offer-benefit-over-the-traditional-broth-microdilution-testing
#2
Melissa R Gitman, Lisa McTaggart, Joanna Spinato, Rahgavi Poopalarajah, Erin Lister, Shahid Husain, Julianne V Kus
Aspergillus spp. cause serious invasive lung infections and Aspergillus fumigatus is the most commonly encountered clinically significant species. Voriconazole is considered to be the drug of choice for A. fumigatus infections, however rising resistance rates have been reported. We evaluated a MALDI-ToF MS-based method for the differentiation between wild type and non-wild type isolates of 20 Aspergillus spp. (including 3 isolates 2 A. ustus, and 1 A. calidoustus that were used as controls due their intrinsic low azole susceptibility with respect to the in vitro response to voriconazole)...
April 12, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28397482/genetic-polymorphism-of-epidermal-growth-factor-rs4444903-influences-susceptibility-to-hcv-related-liver-cirrhosis-and-hepatocellular-carcinoma-in-a-chinese-han-population
#3
Shitian Zhang, Kunyan Qiao, Congdoanh Trieu, Zhixiao Huo, Qinghai Dai, Yanan Du, Wei Lu, Wei Hou
BACKGROUND: Genetic polymorphism in the epidermal growth factor (EGF, rs4444903) gene has been demonstrated to be associated with the clinical deterioration in hepatitis C virus (HCV)-related liver cirrhosis (LC) and the development of hepatocellular carcinoma (HCC). Whether this single nucleotide polymorphism (SNP) influences susceptibility to HCV-related LC and HCC in the Chinese Han population is largely unknown. METHODS: In this case-control study, a total of 187 Chinese Han patients with chronic HCV infection were enrolled, including 62 HCV-related LC patients, 46 HCV-related HCC patients, and 79 chronic hepatitis C (CHC) patients without LC and HCC, and the genetic polymorphism was genotyped via a matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) assay...
April 1, 2017: Clinical Laboratory
https://www.readbyqxmd.com/read/28397475/evaluation-of-maldi-tof-ms-for-the-identification-of-yeast-isolates-causing-bloodstream-infection
#4
Ozge Turhan, Betil Ozhak-Baysan, Oscar Zaragoza Hernandez, Halil Er, Gozde Ongut, Dilara Ogunc, Dilek Colak, Manuel Cuenca-Estrella
BACKGROUND: Infections due to Candida species are major causes of morbidity and mortality in humans, causing a diverse spectrum of clinical disease ranging from superficial and mucosal infections to invasive disease. Several authors have demonstrated that mortality is closely linked to both timing of therapy and/or source control. The rapid identification of pathogenic species is helpful to start timely and effective antifungal therapy. The aim of this study was to assess the performance of the matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) system for the correct and rapid identification of yeast isolates causing bloodstream infection...
April 1, 2017: Clinical Laboratory
https://www.readbyqxmd.com/read/28396286/altered-mitochondrial-and-peroxisomal-integrity-in-lipocalin-2-deficient-mice-with-hepatic-steatosis
#5
Anastasia Asimakopoulou, Annabelle Fülöp, Erawan Borkham-Kamphorst, Eddy Van de Leur, Nikolaus Gassler, Thorsten Berger, Birte Beine, Helmut E Meyer, Tak W Mak, Carsten Hopf, Corinna Henkel, Ralf Weiskirchen
Lipocalin-2 (LCN2) is a secreted adipokine that transports small hydrophobic molecules such as fatty acids and steroids. LCN2 limits bacterial growth by sequestering iron-containing siderophores and in mammalian liver protects against inflammation, infection, injury and other stressors. Because LCN2 modulates hepatic fat metabolism and homeostasis, we performed a comparative profiling of proteins and lipids of wild type (WT) and Lcn2-deficient mice fed either standard chow or a methionine- and choline-deficient (MCD) diet...
April 7, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28371911/molecular-epidemiology-of-a-malassezia-pachydermatis-neonatal-unit-outbreak
#6
Amin Ilahi, Inès Hadrich, Sabrina Goudjil, Guy Kongolo, Christèle Chazal, André Léké, Ali Ayadi, Taieb Chouaki, Stéphane Ranque
The non-lipid-dependent yeast Malassezia pachydermatis is predominantly zoophilic but occasionally colonizes the human skin. This yeast caused an outbreak in a neonatal iIntensive care unit (NICU). This study aimed to describe the molecular epidemiology of this M. pachydermatis outbreak. All the M. pachydermatis isolates collected at a French University Hospital from January 2012 to April 2013 were included in the study. M. pachydermatis isolates, sampled from various biological samples sites in 25 patients, were identified via MALDI-TOF mass spectrometry and typed using intergenic-spacer 1 (IGS1) nucleotide sequence polymorphisms analysis...
March 24, 2017: Medical Mycology: Official Publication of the International Society for Human and Animal Mycology
https://www.readbyqxmd.com/read/28341192/potential-infection-control-risks-associated-with-ultrasound-equipment-a-bacterial-perspective
#7
Susan Campbell Westerway, Jocelyne M Basseal, Adam Brockway, Jon A Hyett, Dee A Carter
Ultrasound equipment used in trans-abdominal (TA) and trans-vaginal (TV) examination may carry bacterial contamination and pose risks to infection control during ultrasound examination. We aimed to describe the prevalence of bacterial contamination on ultrasound probes, gel, machine keyboard and cords and examined the effectiveness of low- and high-level disinfection techniques. This study was performed at a public hospital and a private practice. A total of 171 swabs were analyzed and bacterial species were identified using matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) analysis and polymerase chain reaction (PCR)...
February 2017: Ultrasound in Medicine & Biology
https://www.readbyqxmd.com/read/28303824/matrix-assisted-laser-desorption-ionisation-time-of-flight-mass-spectrometry-rapid-detection-of-carbapenamase-activity-in-acinetobacter-baumannii-isolates
#8
Noha Abouseada, May Raouf, Eman El-Attar, Pacinte Moez
INTRODUCTION: Carbapenamase-producing Acinetobacter baumannii are an increasing threat in hospitals and Intensive Care Units. Accurate and rapid detection of carbapenamase producers has a great impact on patient improvement and aids in implementation of infection control measures. AIM: In this study, we describe the use of matrix-assisted laser desorption ionisation time-of-flight mass spectrometry (MALDI TOF MS) to identify carbapenamase-producing A. baumannii isolates in up to 3 h...
January 2017: Indian Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28285502/potential-vaccine-targets-against-rabbit-coccidiosis-by-immunoproteomic-analysis
#9
Hongyan Song, Ronglian Dong, Baofeng Qiu, Jin Jing, Shunxing Zhu, Chun Liu, Yingmei Jiang, Liucheng Wu, Shengcun Wang, Jin Miao, Yixiang Shao
The aim of this study was to identify antigens for a vaccine or drug target to control rabbit coccidiosis. A combination of 2-dimensional electrophoresis, immunoblotting, and mass spectrometric analysis were used to identify novel antigens from the sporozoites of Eimeria stiedae. Protein spots were recognized by the sera of New Zealand rabbits infected artificially with E. stiedae. The proteins were characterized by matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF/TOF-MS) analysis in combination with bioinformatics...
February 2017: Korean Journal of Parasitology
https://www.readbyqxmd.com/read/28257640/risk-factors-for-candida-parapsilosis-bloodstream-infection-in-a-neonatal-intensive-care-unit-a-case-control-study
#10
Carmine Garzillo, Maria Bagattini, Lidija Bogdanović, Anna Di Popolo, Vita Dora Iula, Maria Rosaria Catania, Francesco Raimondi, Maria Triassi, Raffaele Zarrilli
BACKGROUND: Candida parapsilosis is increasingly responsible for invasive candidiasis in neonates. This study investigates phenotypic and genotypic features of C. parapsilosis microbial isolates and underlying clinical conditions associated with acquisition of C. parapsilosis in a neonatal intensive care unit (NICU) in Italy. METHODS: Identification of C. parapsilosis was performed by VITEK® 2 and MALDI TOF and confirmed by analysis of internal transcribed spacer ribosomal DNA sequences...
January 19, 2017: Italian Journal of Pediatrics
https://www.readbyqxmd.com/read/28246552/decreased-tissue-cox5b-expression-and-mitochondrial-dysfunction-during-sepsis-induced-kidney-injury-in-rats
#11
Jochen Hinkelbein, Lennert Böhm, Stefan Braunecker, Christoph Adler, Edoardo De Robertis, Fabrizio Cirillo
Background. Sepsis is defined as a life-threatening organ dysfunction due to a dysregulated host response to infection. Sepsis is the dominant cause of acute kidney injury (AKI), accounting for nearly 50% of episodes of acute renal failure. Signaling cascades and pathways within the kidney are largely unknown and analysis of these molecular mechanisms may enhance knowledge on pathophysiology and possible therapeutic options. Material and Methods. 26 male Wistar rats were assigned to either a sham group (control, N = 6) or sepsis group (N = 20; cecal ligature and puncture model, 24 and 48 hours after CLP)...
2017: Oxidative Medicine and Cellular Longevity
https://www.readbyqxmd.com/read/28228491/maldi-tof-alone-versus-maldi-tof-combined-with-real-time-antimicrobial-stewardship-interventions-on-time-to-optimal-antimicrobial-therapy-in-patients-with-positive-blood-cultures
#12
Maya Beganovic, Michael Costello, Sarah M Wieczorkiewicz
Introduction: Matrix-assisted laser desorption ionization time of flight (MALDI-TOF) decreases time to organism identification and improves clinical and financial outcomes. The purpose of this study was to evaluate the impact of MALDI-TOF alone vs MALDI-TOF combined with real-time, pharmacist-driven, antimicrobial stewardship (AMS) intervention on patient outcomes.Methods: This single-center, pre-post quasi-experimental study evaluated hospitalized patients with positive blood cultures identified via MALDI-TOF combined with prospective AMS intervention, compared to a control cohort with MALDI-TOF identification without AMS intervention...
February 22, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28219823/proteomic-analysis-of-a-carbapenem-resistant-klebsiella-pneumoniae-strain-in-response-to-meropenem-stress
#13
Arbab Khan, Divakar Sharma, Mohammad Faheem, Deepa Bisht, Asad U Khan
OBJECTIVES: Antibiotic resistance has become a major problem in treating bacterial infections. The aim of this study was to elucidate the effects of meropenem on a blaKPC-2-harbouring multidrug-resistant clinical strain of Klebsiella pneumoniae through a proteomics approach in order to attain a deeper understanding of bacterial resistance strategies. METHODS: Analysis was performed by two-dimensional gel electrophoresis of whole-cell extracts of bacteria exposed to a sublethal concentration of meropenem compared with the untreated control...
February 20, 2017: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/28124968/-accurate-diagnosis-of-pseudomonas-luteola-in-routine-microbiology-laboratory-on-the-occasion-of-two-isolates
#14
Muharrem Çiçek, Gülşen Hasçelik, H Kaan Müştak, K Serdar Diker, Burçin Şener
Pseudomonas luteola which was previously known as Chryseomonas luteola; is a gram-negative, non-fermentative, aerobic, motile, non-spore-forming bacillus. It is frequently found as a saprophyte in soil, water and other damp environments and is an opportunistic pathogen in patients with underlying medical disorders or with indwelling catheters. It has been reported as an uncommon cause of bacteremia, sepsis, septic arthritis, meningitis, endocarditis, and peritonitis. Thus, early and accurate identification of this rare species is important for the treatment and also to provide information about the epidemiology of P...
October 2016: Mikrobiyoloji Bülteni
https://www.readbyqxmd.com/read/28049524/maldi-tof-ms-as-an-innovative-tool-for-detection-of-plasmodium-parasites-in-anopheles-mosquitoes
#15
Maureen Laroche, Lionel Almeras, Emilie Pecchi, Yassina Bechah, Didier Raoult, Angèle Viola, Philippe Parola
BACKGROUND: Malaria is still a major public health issue worldwide, and one of the best approaches to fight the disease remains vector control. The current methods for mosquito identification include morphological methods that are generally time-consuming and require expertise, and molecular methods that require laboratory facilities with relatively expensive running costs. Matrix-Assisted Laser Desorption Ionization Time-Of-Flight Mass Spectrometry (MALDI-TOF MS) technology, routinely used for bacterial identification, has recently emerged in the field of entomology...
January 3, 2017: Malaria Journal
https://www.readbyqxmd.com/read/27955639/control-of-carbapenem-resistant-acinetobacter-baumannii-outbreak-in-an-intensive-care-unit-of-a-teaching-hospital-in-southern-italy
#16
Aida Bianco, Angela Quirino, Mariavalentina Giordano, Vito Marano, Claudia Rizzo, Maria Carla Liberto, Alfredo Focà, Maria Pavia
BACKGROUND: Acinetobacter baumannii is an opportunistic pathogen that has become a major cause of concern, since it is a frequent cause of healthcare-associated infections (HAIs). The aim of the study was to describe the occurrence, the management and the control of an outbreak that occurred in an intensive care unit (ICU) of a teaching hospital in Southern Italy caused by multiple strains of extensively drug-resistant A. baumannii (XDRAB). METHODS: Case-patient was defined as a patient with an healthcare-associated infection caused by an XDRAB isolate identified in a clinically significant culture...
December 12, 2016: BMC Infectious Diseases
https://www.readbyqxmd.com/read/27942042/faecal-carriage-of-gram-negative-multidrug-resistant-bacteria-among-patients-hospitalized-in-two-centres-in-ulaanbaatar-mongolia
#17
Bayaraa Baljin, Ganbaatar Baldan, Battogtokh Chimeddorj, Khosbayar Tulgaa, Batbaatar Gunchin, Tsogtsaikhan Sandag, Klaus Pfeffer, Colin R MacKenzie, Andreas F Wendel
Gram-negative multidrug-resistant organisms (GN-MDRO) producing β-lactamases (ESBL, plasmid-mediated AmpC β-lactamases and carbapenemases) are increasingly reported throughout Asia. The aim of this surveillance study was to determine the rate of bacterial colonization in patients from two hospitals in the Mongolian capital Ulaanbaatar. Rectal swabs were obtained from patients referred to the National Traumatology and Orthopaedics Research Centre (NTORC) or the Burn Treatment Centre (BTC) between July and September 2014, on admission and again after 14 days...
2016: PloS One
https://www.readbyqxmd.com/read/27936983/molecular-diagnosis-of-invasive-mycoses-of-the-central-nervous-system
#18
Matthew William McCarthy, Thomas J Walsh
In September 2012, the Centers for Disease Control and Prevention (CDC) began investigating an outbreak of fungal meningitis among patients who had received contaminated preservative-free methyl prednisolone acetate injections from the New England Compounding Center in Framingham, Massachusetts. Thousands of patients were potentially exposed to tainted corticosteroids, but establishing the diagnosis of fungal meningitis during the nationwide outbreak was difficult because little was known about the natural history of the disease...
February 2017: Expert Review of Molecular Diagnostics
https://www.readbyqxmd.com/read/27936381/a-proteomic-approach-for-rapid-identification-of-weissella-species-isolated-from-korean-fermented-foods-on-maldi-tof-ms-supplemented-with-an-in-house-database
#19
Eiseul Kim, Youngjae Cho, Yoonju Lee, Sun-Kyung Han, Chang-Gyeom Kim, Dong-Won Choo, Young-Rok Kim, Hae-Yeong Kim
Weissella are obligate heterofermentative lactic acid bacteria belonging to the Leuconostocaceae family. Some Weissella can be found in salted and fermented foods, such as kimchi and jeotgal, and plays an important role in the fermentation process. In the present study, for the first time, a rapid and accurate identification method for Weissella species from kimchi and jeotgal was developed based on MALDI-TOF MS, supplemented with an in-house database. Of the 135 Weissella spectra aligned with the MALDI bioTyper database, 56 isolates (41...
February 21, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/27896481/high-perineal-and-overall-frequency-of-staphylococcus-aureus-in-people-who-inject-drugs-compared-to-non-injectors
#20
Disa Dahlman, Farnaz Jalalvand, Marianne Alanko Blomé, Anders Håkansson, Håkan Janson, Susanne Quick, Anna C Nilsson
To investigate the prevalence, distribution, and colonization burden of Staphylococcus aureus (S. aureus) and MRSA in different body sites among people who inject drugs (PWID) and compare it to a control group consisting of non-injectors. In this cross-sectional survey, 49 active PWID from the needle exchange program (NEP) in Malmö, Sweden, and 60 non-injecting controls from an emergency psychiatric inpatient ward at Malmö Addiction Centre were tested for S. aureus (including MRSA) by culture, PCR, and MALDI-TOF...
February 2017: Current Microbiology
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