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https://www.readbyqxmd.com/read/29224733/validated-maldi-tof-ms-method-for-anthrax-lethal-factor-provides-early-diagnosis-and-evaluation-of-therapeutics
#1
Maribel Gallegos-Candela, Anne E Boyer, Adrian R Woolfitt, Judy Brumlow, Renato C Lins, Conrad P Quinn, Alex R Hoffmaster, Gabriel Meister, John R Barr
Anthrax lethal factor (LF) is a zinc-dependent endoprotease and a critical virulence factor for Bacillus anthracis, the causative agent of anthrax. The mass spectrometry (MS) method for total-LF quantification includes three steps; 1) LF specific antibody capture/concentration, 2) LF-specific hydrolysis of a peptide substrate, and 3) detection and quantification of LF-cleaved peptides by isotope-dilution MALDI-TOF/MS. Recombinant LF spiked plasma was used for calibration and quality control (QC) materials. Specificity was 100% from analysis of serum and plasma from 383 non-infected humans, 31 rabbits, and 24 rhesus macaques...
December 7, 2017: Analytical Biochemistry
https://www.readbyqxmd.com/read/29193200/rapid-identification-of-microorganisms-from-platelet-concentrates-by-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry-after-short-term-incubation-on-liquid-medium
#2
Yasmine Chetouane, Gregory Dubourg, Pierre Gallian, Christophe Flaudrops, Jacques Chiaroni, Eric Chabrière, Didier Raoult, Laurence Camoin-Jau
BACKGROUND: Platelets (PLTs) are especially affected by the risk of bacterial contamination. Matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) is an accurate method for the routine identification of bacterial isolates in microbiology laboratories. We directly applied the MALDI-TOF method to bacterial detection in PLTs. In this study, we evaluated the sensitivity, specificity, and speed of a direct MALDI-TOF approach compared to the conventional method BACTEC. STUDY DESIGN AND METHODS: Eight bacteria associated with PLT contamination, cited by the ISBT on transfusion-transmitted infectious diseases, were spiked into PLTs for a final concentration of approximately 100 CFU/bag (n = 5 for each strain)...
November 28, 2017: Transfusion
https://www.readbyqxmd.com/read/29076587/high-prevalence-of-candiduria-due-to-non-albicans-candida-species-among-diabetic-patients-a-matter-of-concern
#3
Alireza Esmailzadeh, Hossein Zarrinfar, AbdolMajid Fata, Tanuka Sen
BACKGROUND: Among the fungal pathogens, Candida species are the most common cause of urinary tract infection (UTI). Some predisposing factors such as diabetes mellitus, urinary retention, urinary stasis, renal transplantation, and hospitalization can increase the risk of candiduria. The aim of this cross-sectional study was to evaluate candiduria among type 2 diabetic patients and identification of the Candida isolates. METHOD: Four hundred clean-catch midstream urine specimens were obtained from patients with type 2 diabetes mellitus...
October 27, 2017: Journal of Clinical Laboratory Analysis
https://www.readbyqxmd.com/read/29055988/-association-of-hla-dq-and-ifnl4-polymorphisms-with-hepatitis-b-virus-infection-and-clearance
#4
H Chen, J H Fan, W X Chen, J J Guo, Y Huang
Objective: To confirmed the polymorphisms of HLA-DQ and IFNL4 were associated with HBV infection and clearance in a Chinese population. Methods: The Sequenom MassARRAY MALDI-TOF system was used to genotype the HLA-DQrs9275319 and IFNL4rs368234815, rs12971396, rs12979860, and rs8099917. A binary logistic regression test was conducted to estimate the relative risk of these SNPs with HBV infection and clearance. Haploview4.2 software and PHASE software (v2.0.2) were employed to analyze linkage disequilibrium (LD) and haplotype frequencies...
July 20, 2017: Zhonghua Gan Zang Bing za Zhi, Zhonghua Ganzangbing Zazhi, Chinese Journal of Hepatology
https://www.readbyqxmd.com/read/29022241/a-panel-of-protein-candidates-for-comprehensive-study-of-caprine-arthritis-encephalitis-cae-infection
#5
Rosivaldo Quirino Bezerra Júnior, Ângela Maria Xavier Eloy, João Ricardo Furtado, Raymundo Rizaldo Pinheiro, Alice Andrioli, Frederico Bruno Moreno, Marina Duarte Pinto Lobo, Ana Cristina O Monteiro-Moreira, Renato de Azevedo Moreira, Tatiana Maria Farias Pinto, Maria Fátima da Silva Teixeira
The caprine arthrite encephalite (CAE) is a disease that affects especially dairy goat. The virus shows compartmentalization features, that allows it to hide at certain times during the course of the disease, making it difficult to control. The present study was conducted to identify the major seminal plasma protein profile of goats infected by CAE and its associations with seroconversion using Western blotting. Two groups containing five males each, were used in this experiment. The first group was composed by seropositive animals and the control by seronegative confirmed by Western blotting and PCR...
October 12, 2017: Tropical Animal Health and Production
https://www.readbyqxmd.com/read/28950023/assessment-of-maldi-tof-ms-biotyping-for-borrelia-burgdorferi-sl-detection-in-ixodes-ricinus
#6
Pierre H Boyer, Nathalie Boulanger, Amira Nebbak, Elodie Collin, Benoit Jaulhac, Lionel Almeras
Matrix Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) has been demonstrated to be useful for tick identification at the species level. More recently, this tool has been successfully applied for the detection of bacterial pathogens directly in tick vectors. The present work has assessed the detection of Borrelia burgdorferi sensu lato in Ixodes ricinus tick vector by MALDI-TOF MS. To this aim, experimental infection model of I. ricinus ticks by B. afzelii was carried out and specimens collected in the field were also included in the study...
2017: PloS One
https://www.readbyqxmd.com/read/28936573/evaluation-of-beauveria-bassiana-infection-in-the%C3%A2-hemolymph-serum-proteins-of-the-housefly-musca-domestica-l-diptera-muscidae
#7
Sapna Mishra, Peeyush Kumar, Anushree Malik
Beauveria bassiana plays a prominent role in biocontrol of houseflies, Musca domestica (L.). Thus, a deeper insight into immune response of M. domestica during B. bassiana infection was warranted to assist the production of more efficient mycoinsecticides. The present study investigates changes in protein profile of M. domestica hemolymph serum post B. bassiana infection using two-dimensional difference gel electrophoresis (2D-DIGE) followed by identification of selected proteins by matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS)...
November 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28811086/proteomic-profiling-of-the-antifungal-drug-response-of-aspergillus-fumigatus-to-voriconazole
#8
Nansalmaa Amarsaikhan, Daniela Albrecht-Eckardt, Christoph Sasse, Gerhard H Braus, Zumrut B Ogel, Olaf Kniemeyer
Antifungal resistance is an emerging problem and one of the reasons for treatment failure of invasive aspergillosis (IA). Voriconazole has become a standard therapeutic for the treatment of this often fatal infection. We studied the differentially expressed proteins as a response of Aspergillus fumigatus to voriconazole by employing the two-dimensional difference gel electrophoresis (DIGE) technique. Due to addition of drug, a total of 135 differentially synthesized proteins were identified by MALDI-TOF/TOF-mass spectrometry...
August 5, 2017: International Journal of Medical Microbiology: IJMM
https://www.readbyqxmd.com/read/28712153/proteomic-analysis-reveals-the-enhancement-of-human-serum-apolipoprotein-a-1-apo-a-1-in-individuals-infected-with-multiple-dengue-virus-serotypes
#9
Nageswar Reddy Manchala, Ranjeet Dungdung, Rajendra Pilankatta
OBJECTIVES: Human serum protein profiling of the individual infected with multiple dengue virus serotypes for identifying the potential biomarkers and to investigate the cause for the severity of dengue virus infection. METHODS: Dengue virus NS1-positive serum samples were pooled into two groups (S2 and S3) based on the molecular serotyping and number of heterotypic infections. The pooled serum samples were subjected to two-dimensional gel electrophoresis (2DGE) to identify the differentially expressed proteins...
October 2017: Tropical Medicine & International Health: TM & IH
https://www.readbyqxmd.com/read/28646681/proteomic-analysis-of-developing-wheat-grains-infected-by-powdery-mildew-blumeria-graminis-f-sp-tritici
#10
Jie Li, Xi-Wen Yang, Yong-Chun Li, Ji-Shan Niu, De-Xian He
Blumeria graminis f.sp. tritici (Bgt) infection greatly interferes with the normal source-sink relationships and always causes tremendous loss of yield and quality in wheat. To better understand the impact of this pathogen on grain development, proteome characterization during grain development in susceptible wheat cultivar Xinong 979 infected by powdery mildew was investigated by 2-DE and tandem MALDI-TOF/TOF-MS. Identification of 111 differentially expressed protein spots representing 85 unique proteins and six expression patterns showed a chronological description of wheat grain formation...
June 10, 2017: Journal of Plant Physiology
https://www.readbyqxmd.com/read/28566082/-femoral-hemodialysis-catheter-related-bacteremia-due-to-globicatella-sanguinis-challenges-in-species-identification
#11
Elif Aktaş, Nafia Canan Gürsoy, Tamer Sakacı, Yener Koç, Aziz Ahmad Hamidi, Emin Bulut, Duygu Erdemir, Barış Otlu
In this case, catheter-related bacteremia due to Globicatella sanguinis in a 43 years old female patient undergoing hemodialysis with the diagnosis of diabetic nephropathy was presented and the methods in the laboratory diagnosis of the rare opportunistic pathogen, Globicatella cins, were nvestigated. A set of peripheral blood cultures and simultaneous catheter culture was obtained from the patient in third of May 2016. Biochemical tests, Phoenix (Becton Dickinson, USA) and MicroScan (Beckman Coulter, USA) automated systems and matrix assisted laser desorption/ionization-time of flight-mass spectrometry (MALDI-TOF MS) based Microflex MS (Bruker, Daltonics, Germany) and VITEK MS (database v2...
April 2017: Mikrobiyoloji Bülteni
https://www.readbyqxmd.com/read/28566081/-a-rarely-isolated-bacterium-in-microbiology-laboratories-streptococcus-uberis
#12
Canan Eryıldız, Şebnem Bukavaz, Şaban Gürcan, Osman Hatipoğlu
Streptococcus uberis is a gram-positive bacterium that is mostly responsible for mastitis in cattle. The bacterium rarely has been associated with human infections. Conventional phenotyphic methods can be inadequate for the identification of S.uberis; and in microbiology laboratories S.uberis is confused with the other streptococci and enterococci isolates. Recently, molecular methods are recommended for the accurate identification of S.uberis isolates. The aim of this report is to present a lower respiratory tract infection case caused by S...
April 2017: Mikrobiyoloji Bülteni
https://www.readbyqxmd.com/read/28430811/antimicrobial-resistance-in-coagulase-positive-staphylococci-isolated-from-companion-animals-in-australia-a-one-year-study
#13
Sugiyono Saputra, David Jordan, Kate A Worthing, Jacqueline M Norris, Hui S Wong, Rebecca Abraham, Darren J Trott, Sam Abraham
Methicillin-resistant coagulase-positive staphylococci (CoPS) have become increasingly recognised as opportunistic pathogens that limit therapeutic options in companion animals. The frequency of methicillin resistance amongst clinical isolates on an Australia-wide level is unknown. This study determined antimicrobial susceptibility patterns for CoPS isolated from clinical infections in companion animals (dogs, cats and horses) as part of the first nation-wide survey on antimicrobial resistance in animal pathogens in Australia for a one-year period (January 2013 to January 2014)...
2017: PloS One
https://www.readbyqxmd.com/read/28404678/antifungal-susceptibility-testing-of-aspergillus-spp-by-using-a-composite-correlation-index-cci-based-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry-method-appears-to-not-offer-benefit-over-traditional-broth-microdilution-testing
#14
Melissa R Gitman, Lisa McTaggart, Joanna Spinato, Rahgavi Poopalarajah, Erin Lister, Shahid Husain, Julianne V Kus
Aspergillus spp. cause serious invasive lung infections, and Aspergillus fumigatus is the most commonly encountered clinically significant species. Voriconazole is considered to be the drug of choice for treating A. fumigatus infections; however, rising resistance rates have been reported. We evaluated a matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS)-based method for the differentiation between wild-type and non-wild-type isolates of 20 Aspergillus spp. (including 2 isolates of Aspergillus ustus and 1 of Aspergillus calidoustus that were used as controls due their intrinsic low azole susceptibility with respect to the in vitro response to voriconazole)...
July 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28397482/genetic-polymorphism-of-epidermal-growth-factor-rs4444903-influences-susceptibility-to-hcv-related-liver-cirrhosis-and-hepatocellular-carcinoma-in-a-chinese-han-population
#15
Shitian Zhang, Kunyan Qiao, Congdoanh Trieu, Zhixiao Huo, Qinghai Dai, Yanan Du, Wei Lu, Wei Hou
BACKGROUND: Genetic polymorphism in the epidermal growth factor (EGF, rs4444903) gene has been demonstrated to be associated with the clinical deterioration in hepatitis C virus (HCV)-related liver cirrhosis (LC) and the development of hepatocellular carcinoma (HCC). Whether this single nucleotide polymorphism (SNP) influences susceptibility to HCV-related LC and HCC in the Chinese Han population is largely unknown. METHODS: In this case-control study, a total of 187 Chinese Han patients with chronic HCV infection were enrolled, including 62 HCV-related LC patients, 46 HCV-related HCC patients, and 79 chronic hepatitis C (CHC) patients without LC and HCC, and the genetic polymorphism was genotyped via a matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) assay...
April 1, 2017: Clinical Laboratory
https://www.readbyqxmd.com/read/28397475/evaluation-of-maldi-tof-ms-for-the-identification-of-yeast-isolates-causing-bloodstream-infection
#16
Ozge Turhan, Betil Ozhak-Baysan, Oscar Zaragoza, Halil Er, Zubeyde Eres Sarıtas, Gozde Ongut, Dilara Ogunc, Dilek Colak, Manuel Cuenca-Estrella
BACKGROUND: Infections due to Candida species are major causes of morbidity and mortality in humans, causing a diverse spectrum of clinical disease ranging from superficial and mucosal infections to invasive disease. Several authors have demonstrated that mortality is closely linked to both timing of therapy and/or source control. The rapid identification of pathogenic species is helpful to start timely and effective antifungal therapy. The aim of this study was to assess the performance of the matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) system for the correct and rapid identification of yeast isolates causing bloodstream infection...
April 1, 2017: Clinical Laboratory
https://www.readbyqxmd.com/read/28396286/altered-mitochondrial-and-peroxisomal-integrity-in-lipocalin-2-deficient-mice-with-hepatic-steatosis
#17
Anastasia Asimakopoulou, Annabelle Fülöp, Erawan Borkham-Kamphorst, Eddy Van de Leur, Nikolaus Gassler, Thorsten Berger, Birte Beine, Helmut E Meyer, Tak W Mak, Carsten Hopf, Corinna Henkel, Ralf Weiskirchen
Lipocalin-2 (LCN2) is a secreted adipokine that transports small hydrophobic molecules such as fatty acids and steroids. LCN2 limits bacterial growth by sequestering iron-containing siderophores and in mammalian liver protects against inflammation, infection, injury and other stressors. Because LCN2 modulates hepatic fat metabolism and homeostasis, we performed a comparative profiling of proteins and lipids of wild type (WT) and Lcn2-deficient mice fed either standard chow or a methionine- and choline-deficient (MCD) diet...
April 7, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28371911/molecular-epidemiology-of-a-malassezia-pachydermatis-neonatal-unit-outbreak
#18
Amin Ilahi, Inès Hadrich, Sabrina Goudjil, Guy Kongolo, Christèle Chazal, André Léké, Ali Ayadi, Taieb Chouaki, Stéphane Ranque
The non-lipid-dependent yeast Malassezia pachydermatis is predominantly zoophilic but occasionally colonizes the human skin. This yeast caused an outbreak in a neonatal iIntensive care unit (NICU). This study aimed to describe the molecular epidemiology of this M. pachydermatis outbreak. All the M. pachydermatis isolates collected at a French University Hospital from January 2012 to April 2013 were included in the study. M. pachydermatis isolates, sampled from various biological samples sites in 25 patients, were identified via MALDI-TOF mass spectrometry and typed using intergenic-spacer 1 (IGS1) nucleotide sequence polymorphisms analysis...
March 24, 2017: Medical Mycology: Official Publication of the International Society for Human and Animal Mycology
https://www.readbyqxmd.com/read/28341192/potential-infection-control-risks-associated-with-ultrasound-equipment-a-bacterial-perspective
#19
Susan Campbell Westerway, Jocelyne M Basseal, Adam Brockway, Jon A Hyett, Dee A Carter
Ultrasound equipment used in trans-abdominal (TA) and trans-vaginal (TV) examination may carry bacterial contamination and pose risks to infection control during ultrasound examination. We aimed to describe the prevalence of bacterial contamination on ultrasound probes, gel, machine keyboard and cords and examined the effectiveness of low- and high-level disinfection techniques. This study was performed at a public hospital and a private practice. A total of 171 swabs were analyzed and bacterial species were identified using matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) analysis and polymerase chain reaction (PCR)...
February 2017: Ultrasound in Medicine & Biology
https://www.readbyqxmd.com/read/28303824/matrix-assisted-laser-desorption-ionisation-time-of-flight-mass-spectrometry-rapid-detection-of-carbapenamase-activity-in-acinetobacter-baumannii-isolates
#20
Noha Abouseada, May Raouf, Eman El-Attar, Pacinte Moez
INTRODUCTION: Carbapenamase-producing Acinetobacter baumannii are an increasing threat in hospitals and Intensive Care Units. Accurate and rapid detection of carbapenamase producers has a great impact on patient improvement and aids in implementation of infection control measures. AIM: In this study, we describe the use of matrix-assisted laser desorption ionisation time-of-flight mass spectrometry (MALDI TOF MS) to identify carbapenamase-producing A. baumannii isolates in up to 3 h...
January 2017: Indian Journal of Medical Microbiology
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