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https://www.readbyqxmd.com/read/29150029/-fungal-keratitis
#1
REVIEW
T Bourcier, A Sauer, A Dory, J Denis, M Sabou
Fungal keratitis, or keratomycoses, are corneal infections which must be considered in cases of corneal trauma, prior corneal surgery, chronic ocular surface disease, topical corticosteroids or contact lens wear. Filamentous fungi or yeasts may be involved. Presenting clinical features such as corneal infiltrates with feathery edges and/or raised surface, intact epithelium with deep stromal involvement, satellite lesions, endothelial plaques, lack of improvement with antibiotics and worsening with steroids are suggestive of fungal keratitis...
November 14, 2017: Journal Français D'ophtalmologie
https://www.readbyqxmd.com/read/29133630/non-contact-lens-related-acanthamoeba-keratitis
#2
REVIEW
Prashant Garg, Paavan Kalra, Joveeta Joseph
The purpose of the study is to describe epidemiology, clinical features, diagnosis, and treatment of Acanthamoeba keratitis (AK) with special focus on the disease in nonusers of contact lenses (CLs). This study was a perspective based on authors' experience and review of published literature. AK accounts for 2% of microbiology-proven cases of keratitis. Trauma and exposure to contaminated water are the main predisposing factors for the disease. Association with CLs is seen only in small fraction of cases. Contrary to classical description experience in India suggests that out of proportion pain, ring infiltrate, and radial keratoneuritis are seen in less than a third of cases...
November 2017: Indian Journal of Ophthalmology
https://www.readbyqxmd.com/read/29097064/acanthamoeba-t4-trophozoites-cross-the-mdck-epithelium-without-cell-damage-but-increase-paracellular-permeability-and-transepithelial-resistance-by-modifying-tight-junction-composition
#3
Catalina Flores-Maldonado, Arturo González-Robles, Lizbeth Salazar-Villatoro, Maritza Omaña-Molina, Juan Manuel Gallardo, Mónica González-Lázaro, Verónica Ivonne Hernández-Ramírez, Patricia Talamás-Rohana, Jacob Lorenzo-Morales, Adolfo Martínez-Palomo
Free-living amoebae of the genus Acanthamoeba are protozoa ubiquitously found in nature. Some species of the genus are potentially pathogenic for humans provoking keratitis in healthy individuals, often in contact lens wearers and opportunistic infections such as pneumonitis, fatal granulomatous encephalitis and skin infections, particularly in immunocompromised individuals. The pathogenic mechanisms of these amoebae are poorly understood, however it had been suggested that contact dependent mechanisms are important during invasion, regardless of the epithelia type, since amoebae penetrate epithelia separating tight junction (TJ)...
October 31, 2017: Experimental Parasitology
https://www.readbyqxmd.com/read/29080613/phylogeography-genetic-variability-and-structure-of-acanthamoeba-metapopulations-in-iran-inferred-by-18s-ribosomal-rna-sequences-a-systematic-review-and-meta-analysis
#4
REVIEW
Adel Spotin, Hamid Reza Moslemzadeh, Mahmoud Mahami-Oskouei, Ehsan Ahmadpour, Maryam Niyyati, Seyed Hossein Hejazi, Fatemeh Memari, Jafar Noori
OBJECTIVE: To verify phylogeography and genetic structure of Acanthamoeba populations among the Iranian clinical isolates and natural/artificial environments distributed in various regions of the country. METHODS: We searched electronic databases including Medline, PubMed, Science Direct, Scopus and Google Scholar from 2005 to 2016. To explore the genetic variability of Acanthamoeba sp, 205 sequences were retrieved from keratitis patients, immunosuppressed cases and environmental sources as of various geographies of Iran...
September 2017: Asian Pacific Journal of Tropical Medicine
https://www.readbyqxmd.com/read/29044738/overview-of-mechanisms-of-antibiotic-resistance-in-pseudomonas-aeruginosa-an-ocular-perspective
#5
REVIEW
Dinesh Subedi, Ajay Kumar Vijay, Mark Willcox
Treatment of Pseudomonas aeruginosa eye infections often becomes a challenge due to the ability of this bacterium to be resistant to antibiotics via intrinsic and acquired mechanisms. Transfer of resistance due to interchangeable genetic elements is an important mechanism for the rapid transfer of antibiotic resistance in this pathogen. As a result, drug-resistant strains are becoming increasingly prevalent worldwide. This review systematically analyses data from recent publications to describe the global prevalence and antibiotic sensitivity of ocular P...
October 18, 2017: Clinical & Experimental Optometry: Journal of the Australian Optometrical Association
https://www.readbyqxmd.com/read/29033270/a-survey-of-contact-lens-related-complications-in-a-tertiary-hospital-in-china
#6
Weiwei Li, Xuguang Sun, Zhiqun Wang, Yang Zhang
PURPOSE: To describe the type of complications related to contact lens (CL) wear in a tertiary hospital in XX, China. METHODS: A retrospective study of 141 patients who complained discomfort after wearing contact lens on an outpatient basis of XX Hospital were conducted from the January 2012 to December 2015. The data included patients' demographics, lens type, history, slit-lamp examination, reports of corneal scrapings, culture, and examination of in vivo confocal microscopy...
October 12, 2017: Contact Lens & Anterior Eye: the Journal of the British Contact Lens Association
https://www.readbyqxmd.com/read/29022964/nocardia-arthritidis-keratitis-case-report-and-review-of-the-literature
#7
Andrew Gieger, Stephen Waller, Joseph Pasternak
INTRODUCTION: Keratitis due to Nocardia infection is not commonly encountered in clinical practice and may therefore be mistaken for fungal or viral keratitis leading to delayed treatment and increased risk of permanent visual impairment. CASE: An otherwise healthy 27 years old Caucasian Active Duty military member presented to the clinic with three days of light sensitivity and irritation of his right eye. He carried a history of PRK in both eyes six years prior and admitted to recent contact lens overuse...
January 2017: Nepalese Journal of Ophthalmology
https://www.readbyqxmd.com/read/28993355/bilateral-fungal-keratitis-with-ring-infiltrates-a-rare-scenario
#8
Manthan Hasmukhbhai Chaniyara, Amar Pujari, Jayanand Urkude, Namrata Sharma
A 12-year-old boy presented to the emergency department with chief complaints of pain, redness, discharge and diminution of vision in both eyes over the previous 20 days. There was no history of preceding trauma, contact lens use, any eye drop usage or ocular surgery. Systemic history was not significant. Presenting uncorrected visual acuity in his right eye was counting fingers at 1 m and 20/200 in the left eye, with accurate projection of rays in both eyes. Slit lamp biomicroscopy showed the presence of bilateral diffuse conjunctival congestion, corneal ring infiltrates and epithelial defect with corneal oedema...
October 9, 2017: BMJ Case Reports
https://www.readbyqxmd.com/read/28987448/fungal-keratitis
#9
REVIEW
T Bourcier, A Sauer, A Dory, J Denis, M Sabou
Fungal keratitis, or keratomycoses, are corneal infections which must be considered in cases of corneal trauma, prior corneal surgery, chronic ocular surface disease, topical corticosteroids or contact lens wear. Filamentous fungi or yeasts may be involved. Presenting clinical features such as corneal infiltrates with feathery edges and/or raised surface, intact epithelium with deep stromal involvement, satellite lesions, endothelial plaques, lack of improvement with antibiotics and worsening with steroids are suggestive of fungal keratitis...
November 2017: Journal Français D'ophtalmologie
https://www.readbyqxmd.com/read/28965795/acanthamoeba-keratitis-in-noncompliant-soft-contact-lenses-users-genotyping-and-risk-factors-a-study-from-cairo-egypt
#10
Eman E Taher, Eman M H Méabed, Islam Abdallah, Wafaa Y Abdel Wahed
Acanthamoeba keratitis (AK) is a severe corneal infection that may occur as a serious outcome of improper use of contact lenses (CL). OBJECTIVES: The study aimed to diagnose AK in soft CL users presenting with infectious keratitis, and to identify the prevalent genotypes isolated from different cases. Another aim was to determine the CL hygiene-related risk behaviors, and to explore the risk of water exposure for developing AK. METHODS: A cross sectional study was performed...
September 28, 2017: Journal of Infection and Public Health
https://www.readbyqxmd.com/read/28954958/role-of-fatty-acid-salts-as-anti-acanthamoeba-agents-for-disinfecting-contact-lens
#11
Aya Tanaka, Mariko Era, Yumeho Obata, Manami Masuda, Takayoshi Kawahara, Takahide Kanyama, Hiroshi Morita
 Acanthamoeba is found in seawater, fresh water, and soil and is an opportunistic pathogen that causes a potentially blinding corneal infection known as Acanthamoeba keratitis. The anti-amoeba activity of 9 fatty acid salts (potassium butyrate (C4K), caproate (C6K), caprylate (C8K), caprate (C10K), laurate (C12K), myristate (C14K), oleate (C18:1K), linoleate (C18:2K), and linolenate (C18:3K)) was tested on Acanthamoeba castellanii ATCC 30010 (trophozoites and cysts). Fatty acid salts (350 mM and pH 10.5) were prepared by mixing fatty acids with the appropriate amount of KOH...
2017: Biocontrol Science
https://www.readbyqxmd.com/read/28919334/failure-of-molecular-diagnostics-of-a-keratitis-inducing-acanthamoeba-strain
#12
Patrick L Scheid, Carsten Balczun
An otherwise healthy 49-year-old female patient presented at the local hospital with severe keratitis in both inflamed eyes. She was a contact lens wearer and had no history of a corneal trauma. In our laboratory for medical parasitology Acanthamoebae were detected microscopically from the cornea scraping and from the fluid of the contact lens storage case after xenical culture and showed the typical cyst morphology of Acanthamoebae group II. The diagnosis of "Acanthamoeba keratitis" was established and successful therapy was provided...
September 14, 2017: Experimental Parasitology
https://www.readbyqxmd.com/read/28919333/status-of-the-effectiveness-of-contact-lens-disinfectants-in-malaysia-against-keratitis-causing-pathogens
#13
Farhat Abjani, Naveed Ahmed Khan, Suk Yul Jung, Ruqaiyyah Siddiqui
The aim of this study was (i) to assess the antimicrobial effects of contact lens disinfecting solutions marketed in Malaysia against common bacterial eye pathogens and as well as eye parasite, Acanthamoeba castellanii, and (ii) to determine whether targeting cyst wall would improve the efficacy of contact lens disinfectants. Using ISO 14729 Stand-Alone Test for disinfecting solutions, bactericidal and amoebicidal assays of six different contact lens solutions including Oxysept(®), AO SEPT PLUS, OPTI-FREE(®) pure moist(®), Renu(®) fresh™, FreshKon(®) CLEAR and COMPLETE RevitaLens™ were performed using Manufacturers Minimum recommended disinfection time (MRDT)...
September 15, 2017: Experimental Parasitology
https://www.readbyqxmd.com/read/28902722/diagnostic-approach-to-ocular-infections-using-various-techniques-from-conventional-culture-to-next-generation-sequencing-analysis
#14
Hiroshi Eguchi, Fumika Hotta, Tomomi Kuwahara, Haruyuki Imaohji, Chika Miyazaki, Miou Hirose, Shunji Kusaka, Masahiko Fukuda, Yoshikazu Shimomura
Ocular infection is caused by both endogenous (resident) and exogenous (environmental) microbes. As the ocular surface interacts with both outer environment and its own resident microbiota, clinical ocular samples are predicted to contain a diverse set of microorganisms. Microscopy of sample smears is an important step in the diagnostic process of infectious diseases to interpret the culture results. Traditional culture techniques have several limitations in the detection and/or identification of uncharacterized bacteria of environmental origin...
November 2017: Cornea
https://www.readbyqxmd.com/read/28873175/development-of-a-poly-%C3%AE%C2%B5-lysine-contact-lens-as-a-drug-delivery-device-for-the-treatment-of-fungal-keratitis
#15
Andrew G Gallagher, Keri McLean, Rosalind M K Stewart, Don A Wellings, Heather E Allison, Rachel L Williams
Purpose: The purpose of this study was to develop a more efficient drug delivery device to overcome the limitations of current drop therapy for the treatment of fungal keratitis. Methods: Amphotericin B (AmpB), 0 to 30 μg/mL, was associated with a poly-ε-lysine (pεK) hydrogel. Fungicidal effect against Candida albicans was assessed at 18 and 42 hours by optical density (OD600) and growth on agar. Tear film dilution effect was mimicked by storage of AmpB pεK gels in 3...
September 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/28844988/risk-factors-demographics-and-clinical-profile-of-acanthamoeba-keratitis-in-melbourne-an-18-year-retrospective-study
#16
Matthew Hao Lee, Robin Geoffrey Abell, Biswadev Mitra, Merv Ferdinands, Rasik B Vajpayee
PURPOSE: To assess incidence, risk factors, presentation and final visual outcome of patients with Acanthamoebakeratitis (AK) treated at the Royal Victorian Eye and Ear Hospital (RVEEH), Melbourne, Australia, over an 18-year period. METHODS: A retrospective review of all cases of AK managed at RVEEH between January 1998 and May 2016 was performed. Data collected included age, gender, affected eye, signs and symptoms, time between symptoms and diagnosis, risk factors, presenting and final visual acuity (VA), investigations, medical treatment, surgical interventions and length of follow-up...
August 27, 2017: British Journal of Ophthalmology
https://www.readbyqxmd.com/read/28834815/effect-of-multipurpose-solution-combined-with-autophagy-inhibitors-on-adhesion-of-acanthamoeba-trophozoites-to-silicone-hydrogel-contact-lenses
#17
Seung-Mok Lee, Da-In Lee, Sung-Hee Park, Hak-Sun Yu, Ji-Eun Lee, Jong-Soo Lee
PURPOSE: The aim of the study was to evaluate the effect of multipurpose contact lens (CL) solution (MPS) combined with autophagy inhibitors on the adhesion properties of Acanthamoeba castellanii (AC) trophozoites to silicone hydrogel CLs. METHODS: AC trophozoites were inoculated onto discs trimmed from a silicone hydrogel CL with plasma surface treatment. After 18 hours, the number of adherent AC trophozoites on the CL treated with MPS was counted under phase-contrast microscopy...
December 2017: Cornea
https://www.readbyqxmd.com/read/28815736/the-diagnosis-and-management-of-contact-lens-related-microbial-keratitis
#18
REVIEW
Nicole Carnt, Chameen Samarawickrama, Andrew White, Fiona Stapleton
Contact lens-associated microbial keratitis poses a diagnostic dilemma for optometrists on two fronts. The distinction between sterile inflammation and microbial infection is often blurred. In addition, there is a requirement with nearly 50 per cent of the Australian and New Zealand optometric profession being therapeutically endorsed, to distinguish between cases of infection that can be managed in the community verses those that require escalation to public hospitals that have access to laboratory diagnostic tools and advanced imaging techniques, such as in vivo confocal microscopy...
August 16, 2017: Clinical & Experimental Optometry: Journal of the Australian Optometrical Association
https://www.readbyqxmd.com/read/28813424/risk-factors-and-causative-organisms-in-microbial-keratitis-in-daily-disposable-contact-lens-wear
#19
MULTICENTER STUDY
Fiona Stapleton, Thomas Naduvilath, Lisa Keay, Cherry Radford, John Dart, Katie Edwards, Nicole Carnt, Darwin Minassian, Brien Holden
PURPOSE: This study investigated independent risk factors and causative organisms in microbial keratitis in daily disposable contact lens (CL)-wearers. METHODS: A multisite prospective case-control study was undertaken. Cases were daily disposable CL-wearers attending Moorfields Eye Hospital with microbial keratitis and those reported through a one-year surveillance study in Australia and in New Zealand. A population-based telephone survey identified daily disposable CL-wearing controls...
2017: PloS One
https://www.readbyqxmd.com/read/28747368/fusarium-keratitis-in-germany
#20
Grit Walther, Serena Stasch, Kerstin Kaerger, Axel Hamprecht, Mathias Roth, Oliver A Cornely, Gerd Geerling, Colin R Mackenzie, Oliver Kurzai, Marie von Lilienfeld-Toal
Fusarium keratitis is a destructive eye infection that is difficult to treat and results in poor outcome. In tropical and subtropical areas, the infection is relatively common and associated with trauma or chronic eye diseases. However, in recent years, an increased incidence has been reported in temperate climate regions. At the German National Reference Center, we have observed a steady increase in case numbers since 2014. Here, we present the first German case series of eye infections with Fusarium species...
October 2017: Journal of Clinical Microbiology
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