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Renjie Wu, Ling-Xian Yi, Lin-Feng Yu, Jing Wang, Yiyun Liu, Xiaojie Chen, Luchao Lv, Jun Yang, Jian-Hua Liu
The objective of this study was to assess the impact of diverse plasmids bearing colistin resistance gene mcr-1 on host fitness. Forty-seven commensal E. coli isolates recovered from the pig farm where mcr-1 was first identified were screened for mcr-1. mcr-1 -bearing plasmids were characterized by sequencing. The fitness impact of mcr-1 -bearing plasmids was evaluated by in vitro competition assays. Twenty-seven (57.5%) E. coli isolates were positive for mcr-1 . The mcr-1 genes were mainly located on plasmids belonging to IncI2 ( n = 5), IncX4 ( n = 11), IncHI2/ST3 ( n = 8), IncFII ( n = 2), and IncY ( n = 2)...
2018: Frontiers in Microbiology
Congming Wu, Yingchao Wang, Xiaomin Shi, Shuang Wang, Hongwei Ren, Zhangqi Shen, Yang Wang, Juchun Lin, Shaolin Wang
Extended-spectrum beta-lactamase-producing Escherichia coli (ESBL-EC) strains are emerging around the world as a source of resistance to β-lactam antibiotics such as ampicillin, cefotaxime, and ceftazidime. mcr-1 is a novel plasmid-mediated gene conferring resistance to colistin. The aim of this study was to investigate the prevalence of ESBL-EC mcr-1 of chicken origin in the different provinces of China during 2008-2014. Overall, 341 of 821 isolates were determined to be ESBL-EC strains, and the proportion of ESBL-positive strains almost doubled from 2008 to 2014...
March 14, 2018: Emerging Microbes & Infections
Giancarlo Ripabelli, Manuela Tamburro, Giuliana Guerrizio, Incoronata Fanelli, Romeo Flocco, Massimiliano Scutellà, Michela L Sammarco
Antimicrobial-resistant Klebsiella pneumoniae represent a global public health concern. K. pneumoniae strains isolated during 2010 and 2014-2016 within a single hospital of Molise Region, Central Italy, were analyzed testing antimicrobial susceptibility, clonality by pulsed-field gel electrophoresis (PFGE) and random amplified polymorphic DNA (RAPD)-PCR, and prevalence of carbapenem resistance genes by PCR. Forty isolates (23 wild-type in 2010 and 17 non-wild-type in 2014-2016) were collected from hospitalized patients (65...
March 9, 2018: Current Microbiology
Ulises Garza-Ramos, Elsa Tamayo-Legorreta, Doris María Arellano-Quintanilla, Nadia Rodriguez-Medina, Jesús Silva-Sanchez, Juan Catalan-Najera, Marisol Karina Rocha-Martínez, María Asunción Bravo-Díaz, Celia Alpuche-Aranda
A colistin-resistant mcr-1 -carrying Escherichia coli strain, RC2-007, was isolated from a swine farm in Mexico. This extraintestinal and uropathogenic strain of E. coli belongs to serotype O89:H9 and sequence type 744. Assembly and annotation resulted in a 4.9-Mb draft genome that revealed the presence of plasmid-mediated mcr-1 -IS ApI1 genes as part of a prophage.
March 8, 2018: Genome Announcements
Konrad Gwozdzinski, Saina Azarderakhsh, Can Imirzalioglu, Linda Falgenhauer, Trinad Chakraborty
The plasmid-located colistin resistance gene ( mcr - 1 ) confers low-level resistance to colistin, a last-line antibiotic against multidrug-resistant Gram-negative bacteria. Current EUCAST/CLSI recommendations require the use of a broth microdilution (BMD) method in cation-adjusted Mueller-Hinton (CA-MH) for colistin susceptibility testing, but approximately 15% of all MCR-1-producers classify as sensitive in this medium. Here we report on an improved calcium-enhanced Mueller-Hinton (CE-MH) medium that permits simple and reliable determination of mcr-1 -producing Enterobacteriaceae Colistin susceptibility for 50 mcr-1 -encoding isolates of E...
March 7, 2018: Journal of Clinical Microbiology
Sue C Nang, Faye C Morris, Michael J McDonald, Mei-Ling Han, Jiping Wang, Richard A Strugnell, Tony Velkov, Jian Li
Objectives: The discovery of mobile colistin resistance mcr-1, a plasmid-borne polymyxin resistance gene, highlights the potential for widespread resistance to the last-line polymyxins. In the present study, we investigated the impact of mcr-1 acquisition on polymyxin resistance and biological fitness in Klebsiella pneumoniae. Methods: K. pneumoniae B5055 was used as the parental strain for the construction of strains carrying vector only (pBBR1MCS-5) and mcr-1 recombinant plasmids (pmcr-1)...
March 5, 2018: Journal of Antimicrobial Chemotherapy
Qun Li, Hong Wang, Yanmei Xu, Xiangning Bai, Jianping Wang, Zhengdong Zhang, Xiang Liu, Yimao Miao, Ling Zhang, Xinqiong Li, Nianli Zou, Guodong Yan, Xi Chen, Jie Zhang, Shanshan Fu, Ruyue Fan, Jianguo Xu, Juan Li, Yanwen Xiong
Escherichia albertii is an emerging member of the Enterobacteriaceae causing human and animal enteric infections. Antimicrobial resistance among enteropathogens has been reported to be increasing in the past years. The purpose of this study was to investigate antibiotic resistance and resistance genes in E. albertii isolated from Zigong city, Sichuan province, China. The susceptibility to 21 antimicrobial agents was determined by Kirby-Bauer disk diffusion method. The highest prevalence was tetracycline resistance with a rate of 62...
2018: Frontiers in Microbiology
Qiuxia Ma, Yong Huang, Jian Wang, Xuebin Xu, Jane Hawkey, Chaojie Yang, Beibei Liang, Xiaofeng Hu, Fuli Wu, Xiaoxia Yang, Jinyan Wang, Rongrui Li, Peng Li, Jing Xie, Leili Jia, Ligui Wang, Rongzhang Hao, Yigang Tong, Kathryn E Holt, Shaofu Qiu, Yansong Sun, Hongbin Song
Since the plasmid-mediated polymyxin resistance gene mcr-1 was first reported in Escherichia coli and Klebsiella pneumoniae in China, only one mcr-1-positive isolate of Shigella sonnei, containing inactivated mcr-1, has been reported worldwide. Here, we screened 1650 historical S. sonnei strains isolated from 2003 to 2015 in China for mcr-1. We determined the antimicrobial susceptibilities and resistance genes of mcr-1-positive isolates and investigated the transferability of polymyxin resistance by plasmid conjugation...
February 28, 2018: International Journal of Antimicrobial Agents
Beibei Liang, Adam P Roberts, Xuebin Xu, Chaojie Yang, Xiaoxia Yang, Jinyan Wang, Shengjie Yi, Yongrui Li, Qiuxia Ma, Fuli Wu, Shaofu Qiu, Hongbin Song
Since its initial discovery in an Escherichia coli isolate from China, mcr-1 has also been detected in Klebsiella pneumoniae and Salmonella enterica , and is rarely reported in other Enterobacteriaceae. Here, we report the isolation and identification of a Shigella flexneri strain harboring mcr-1 from stool samples in a pig farm in China from 2009. The minimum inhibitory concentration (MIC) to colistin of the isolate is 4 μg/mL. Conjugation assays showed the donor S. flexneri strain has functional and transferable colistin resistance...
March 2, 2018: Applied and Environmental Microbiology
Jilei Zhang, Li Chen, Jiawei Wang, Afrah Kamal Yassin, Patrick Butaye, Patrick Kelly, Jiansen Gong, Weina Guo, Jing Li, Min Li, Feng Yang, Zhixing Feng, Ping Jiang, Chunlian Song, Yaoyao Wang, Jinfeng You, Yi Yang, Stuart Price, Kezong Qi, Yuan Kang, Chengming Wang
Antimicrobial resistance against colistin has emerged worldwide and is threatening the efficacy of colistin treatment of multi-resistant Gram-negative bacteria. In this study, PCRs were used to detect mcr genes (mcr-1, mcr-2, mcr-3) in 213 anal and 1,339 nasal swabs from pigs (n = 1,454) in nine provinces of China, and 1,696 cloacal and 1,647 oropharyngeal samples from poultry (n = 1,836) at live-bird markets in 24 provinces. The mcr-1 prevalences in pigs (79.2%) and geese (71.7%) were significantly higher than in chickens (31...
February 27, 2018: Scientific Reports
Ji-Young Lee, Suk-Kyung Lim, Yujin Choi, Dong-Chan Moon, Juyoun Shin, Kwan Soo Ko
Of 11 mcr-1-harboring plasmids previously identified from livestock in Korea, we performed whole plasmid sequencing on 3 plasmids and determined the genetic structure surrounding mcr-1 for all 11 plasmids. Transconjugation frequencies were measured for all mcr-1-harboring plasmids and competitive growth experiments were performed to investigate the fitness cost of each plasmid. Although they belong to different clones, the mcr-1-harboring plasmids, pEC006 and pEC019, were highly similar to the first identified mcr-1-carrying Incl2-type plasmid, pHNSHP45...
February 27, 2018: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
Francis Onwugamba, J Ross Fitzgerald, Kateryn Rochon, Luca Guardabassi, Abraham Alabi, Stefan Kühne, Martin P Grobusch, Frieder Schaumburg
BACKGROUND: 'Filth flies' feed and develop in excrement and decaying matter and can transmit enteric pathogens to humans and animals, leading to colonization and infection. Considering these characteristics, 'filth flies' are potential vectors for the spread of antimicrobial resistance (AMR). This review defines the role of flies in the spread of AMR and identifies knowledge gaps. METHODS: The literature search (original articles, reviews indexed for PubMed) was restricted to the English language...
February 23, 2018: Travel Medicine and Infectious Disease
D Lepelletier, R Bonnet, P Plésiat, M H Nicolas-Chanoine, A Berger-Carbonne, C Chidiac, B Grandbastien
The increasing use of colistin has contributed to the emergence of resistant bacteria and to an increase in the frequency of infections caused by naturally resistant Enterobacteriaceae strains such as Proteus, Providencia, Morganella, and Serratia. In August 2016, the French High Council for Public Health (French acronym HCSP) received a request from the Ministry of Health on the advice of the French National Public Health agency (Santé publique France) with regard to measures that should be taken to tackle the emergence of plasmid-mediated colistin resistance among Enterobacteriaceae strains...
February 20, 2018: Médecine et Maladies Infectieuses
Dongdong Zhao, Zhihui Zhou, Xiaoting Hua, Huichuan Zhang, Jingjing Quan, Xi Li, Yunsong Yu
Here we report the first clinical Escherichia coli isolate co-harboring mcr-1, blaKPC-2 and two copies of fosA3 from China. The five plasmids of the isolate were completely sequenced and analyzed. Gene mcr-1 and blaKPC-2 were located on IncI2 and IncR plasmid, respectively. A variety of other resistance determinants such as fosA3 (two copies), blaCTX-M-123 , blaOXA-1 and blaCTX-M-65 were also identified from the rest plasmids.
February 19, 2018: Infection, Genetics and Evolution
Bin Huang, Yuting He, Xingyan Ma, Renxin Cai, Jianming Zeng, Yang Lu, Weizheng Zhang, Kai Lan, Sunmei E, Yi-Wei Tang, Barry N Kreiswirth, Cha Chen, Liang Chen
Next generation sequencing of six mcr-1- harboring Escherichia coli and Klebsiella pneumoniae isolates collected from a tertiary care hospital in China revealed significant sequence variations in the regions flanking the mcr-1 gene. While sequence variations significantly affect the expression and promoter activity of mcr-1 , the mcr-1 gene expressions do not correlate with the in vitro colistin resistance levels, which warrants further in-depth investigations.
February 20, 2018: Antimicrobial Agents and Chemotherapy
Jilei Zhang, Li Chen, Jiawei Wang, Patrick Butaye, Ke Huang, Haixiang Qiu, Xiaomei Zhang, Weijuan Gong, Chengming Wang
OBJECTIVE: Colistin resistance has emerged worldwide and has been threatening the efficacy of one of the last-resort antimicrobials used for treatment of multidrug resistant Gram-negative bacteria. While five colistin resistance genes (mcr-1, mcr-2, mcr-3, mcr-4 and mcr-5) have been described, few data are available on the prevalence of mcr-genes other than mcr-1 in human samples. RESULTS: In this study, the presence of five currently described colistin resistance genes (mcr 1-5) in vaginal swabs of women undergoing infertility evaluation was reported...
February 20, 2018: BMC Research Notes
Yongchang Xu, Jingxia Lin, Tao Cui, Swaminath Srinivas, Youjun Feng
Polymyxins such as colistin are antibiotics used as a final line of defense in the management of infections with multidrug-resistant Gram-negative bacteria. Although natural resistance to polymyxins is rare, the discovery of a mobilized colistin resistance gene ( mcr-1 ) in gut bacteria has raised significant concern. As an intramembrane enzyme, MCR-1 catalyzes the transfer of phosphoethanolamine (PEA) to the 1 (or 4')-phosphate group of the lipid A moiety of lipopolysaccharide (LPS), thereby conferring colistin resistance...
February 9, 2018: Journal of Biological Chemistry
F Del Bianco, M Morotti, M F Pedna, P Farabegoli, V Sambri
OBJECTIVES: To start a surveillance program to investigate the possible diffusion of mobilized colistin resistance genes in Enterobacteriaceae strains isolated in the Unit of Microbiology of the Great Romagna Hub Laboratory. METHODS: All the colistin resistant Enterobacteriaceae, isolated from August 1st 2016 to July 31st 2017, were prospectively evaluated for mcr-1 a mcr-2. Backdated survey of mcr-3, mcr-4 and mcr-5 was performed on the same group of isolates. Species identification was achieved by Vitek MS and the antibiotic susceptibility testing was performed both with Vitek-2 and Sensititre systems...
February 12, 2018: International Journal of Infectious Diseases: IJID
Wai-Sing Chan, Chun-Hang Au, Dona N Ho, Tsun-Leung Chan, Edmond Shiu-Kwan Ma, Bone Siu-Fai Tang
BACKGROUND: Human fecal carriage of Enterobacteriaceae possessing mobilized colistin resistance genes (mcr-1 and mcr-2) remains obscure in Hong Kong. As part of routine surveillance on emerging antibiotic resistance, we conducted a prospective study on this topic in a regional hospital in Hong Kong. METHODS: From October 31 to November 25, 2016, all fecal specimens submitted for routine analysis were included in this surveillance study. These comprised 672 consecutive routine fecal specimens collected from 616 individuals...
February 13, 2018: BMC Infectious Diseases
Yi-Ming Wang, Ling-Cong Kong, Jie Liu, Hong-Xia Ma
Background: Bacterial infections have become more challenging to treat due to the emergence of multidrug-resistant pathogenic bacteria. Combined antibiotics prove to be a relatively effective method to control such resistant strains. This study aim to investigate synergistic activity of eugenol combined with colistin against a collection of clinical isolated Escherichia coli (E.coli) strains, and to evaluate potential interaction. Methods: Antimicrobial susceptibility, minimum inhibitory concentration (MIC) and fractional inhibitory concentration index (FICI) of the bacteria were determined by disk diffusion assay, broth microdilution method and checkerboard assay, respectively...
2018: Antimicrobial Resistance and Infection Control
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