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https://www.readbyqxmd.com/read/29785534/an-enzyme-free-homogenous-electrochemical-assay-for-sensitive-detection-of-the-plasmid-mediated-colistin-resistance-gene-mcr-1
#1
Bo Li, Zhixin Chai, Xiaohui Yan, Chunchen Liu, Bo Situ, Ye Zhang, Weilun Pan, Shihua Luo, Jianhua Liu, Lei Zheng
Antibiotic resistance associated with the mcr-1 gene of Gram-negative bacteria, which confers resistance to drugs of last resort and has the potential to spread via plasmids, is one of the most pressing issues facing global health today. Point-of-care testing for the mcr-1 gene is needed to aid in the identification of colistin resistance in the field and to control its horizontal transmission. Here, we report the successful development of an enzyme-free homogenous electrochemical strategy for sensitive detection of the antibiotic resistance gene mcr-1 using the hybridization chain reaction and mcr-1-specific toehold probe...
May 22, 2018: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/29782210/colonization-with-enterobacteriaceae-producing-esbls-ampcs-and-oxa-48-in-wild-avian-species-spain-2015-2016
#2
Jesús Oteo, Aida Mencía, Verónica Bautista, Natalia Pastor, Noelia Lara, Fernando González-González, Francisco Javier García-Peña, José Campos
Antibiotic resistance is a global threat of complex and changeable epidemiology. The role of wild birds in the dissemination of antibacterial resistance might be underestimated. We studied the cloacal colonization by cefotaxime-resistant Enterobacteriaceae in 668 wild birds in Spain. Eighty-eight wild birds (13.2%) of 28 species carried cefotaxime-resistant isolates; 58 of them (8.7%) carried extended-spectrum β-lactamases (ESBLs) and 15 (2.5%) plasmid-mediated AmpCs of the blaCIT family. The 58 ESBLs belonged to the CTX-M-1 group (63...
May 21, 2018: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/29778190/emergence-of-the-colistin-resistance-gene-mcr-1-and-its-variant-in-several-uncommon-species-of-enterobacteriaceae-from-commercial-poultry-farm-surrounding-environments
#3
Xiaoming Wang, Yao Wang, Yang Wang, Suxia Zhang, Zhangqi Shen, Shaolin Wang
The colistin resistance gene mcr-1 has been detected in multiple members of Enterobacteriaceae family. Here, we report the emergence of mcr-1 in Providencia alcalifaciens and a mcr-1 variant, named mcr-1.3, in Raoultella planticola. Both of the mcr-1-carrying plasmids in these two isolates belong to IncI2 type of plasmids, but they are different in sizes and genetic characteristics. We also detected the mcr-1 gene in one Enterobacter cloacae isolate, however, the mcr-1-carrying plasmid is distinct from the previous reports...
June 2018: Veterinary Microbiology
https://www.readbyqxmd.com/read/29774212/alkaline-peptone-water-based-enrichment-method-for-mcr-3-from-acute-diarrheic-outpatient-gut-samples
#4
Qiaoling Sun, Yanyan Hu, Hongwei Zhou, Lingbin Shu, Hanyu Wang, Zixian Huang, Rong Zhang
A third plasmid-mediated colistin resistance gene, mcr-3 , is increasingly being reported in Enterobacteriaceae and Aeromonas spp. from animals and humans. To investigate the molecular epidemiology of mcr in the gut flora of Chinese outpatients, 152 stool specimens were randomly collected from outpatients in our hospital from May to June, 2017. Stool specimens enriched in alkaline peptone water or Luria-Bertani (LB) broth were screened for mcr-1, mcr-2 , and mcr-3 using polymerase chain reaction (PCR)-based assays...
2018: Frontiers in Medicine
https://www.readbyqxmd.com/read/29768099/the-mcr-1-gene-has-no-effect-on-colistin-resistance-when-it-coexists-with-inactivated-mgrb-gene-in-klebsiella-pneumoniae
#5
Huichuan Zhang, Dongdong Zhao, Qiucheng Shi, Jingjing Quan, Xi Li, Yunsong Yu
The inactivated mgrB gene and the mcr-1 gene are important mechanisms of colistin resistance in Klebsiella pneumoniae and they are threats to the clinical use of colistin. In this study, mcr-1 gene was cloned into K. pneumoniae strains (XH209 and KP10) and their derived strains (XH209 M and KP10 M), which showed high-level resistance to colistin. The acquisition of the mcr-1 gene led to colistin resistance in XH209 and KP10, but the addition of mcr-1 gene did not cause change of colistin minimum inhibitory concentrations in the XH209 M and KP10 M...
May 16, 2018: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/29760138/co-location-of-the-polymyxin-resistance-gene-mcr-1-and-variant-of-mcr-3-on-a-plasmid-in-escherichia-coli-from-chicken-farm
#6
Rong Xiang, Bi-Hui Liu, An-Yun Zhang, Chang-Wei Lei, Xiao-Lan Ye, Yan-Xian Yang, Yan-Peng Chen, Hong-Ning Wang
A colistin-resistant Escherichia coli from commercial poultry farm in China carried two colistin-resistance genes mcr-1 and variant of mcr-3 in an IncP plasmid. The variant of mcr-3 gene, named mcr-3.11 , encoded two amino acid substitutions compared with the mcr-3 A novel genetic structure, IS Kpn40-mcr-3-dgkA - IS Kpn40 , might be the key element mediating translocation of mcr-3 through the formation of circular form. The mcr-1 and mcr-3 genes co-located on a plasmid might pose huge threat to public health...
May 14, 2018: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/29758020/defining-icr-mo-an-intrinsic-colistin-resistance-determinant-from-moraxella-osloensis
#7
Wenhui Wei, Swaminath Srinivas, Jingxia Lin, Zichen Tang, Shihua Wang, Saif Ullah, Vishnu Goutham Kota, Youjun Feng
Polymyxin is the last line of defense against severe infections caused by carbapenem-resistant gram-negative pathogens. The emergence of transferable MCR-1/2 polymyxin resistance greatly challenges the renewed interest in colistin (polymyxin E) for clinical treatments. Recent studies have suggested that Moraxella species are a putative reservoir for MCR-1/2 genetic determinants. Here, we report the functional definition of ICR-Mo from M. osloensis, a chromosomally encoded determinant of colistin resistance, in close relation to current MCR-1/2 family...
May 14, 2018: PLoS Genetics
https://www.readbyqxmd.com/read/29755416/complete-genomic-analysis-of-a-salmonella-enterica-serovar-typhimurium-isolate-cultured-from-ready-to-eat-pork-in-china-carrying-one-large-plasmid-containing-mcr-1
#8
Wei Wang, Zulqarnain Baloch, Mingyuan Zou, Yinping Dong, Zixin Peng, Yujie Hu, Jin Xu, Nafeesa Yasmeen, Fengqin Li, Séamus Fanning
One mcr-1 -carrying ST34-type Salmonella Typhimurium WW012 was cultured from 3,200 ready-to-eat (RTE) pork samples in 2014 in China. Broth dilution method was applied to obtain the antimicrobial susceptibility of Salmonella Typhimurium WW012. Broth matting assays were carried out to detect transferability of this phenotype and whole-genome sequencing was performed to analyze its genomic characteristic. Thirty out of 3,200 RTE samples were positive for Salmonella and the three most frequent serotypes were identified as S ...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29741287/longitudinal-study-on-the-occurrence-in-pigs-of-colistin-resistant-e-coli-carrying-mcr-1-following-the-cessation-of-use-of-colistin
#9
Luke P Randall, Robert A Horton, Fabrizio Lemma, Francesca Martelli, Nick A D Duggett, Richard P Smith, Miranda J Kirchner, Richard J Ellis, Jon P Rogers, Susanna M Williamson, Robin R L Simons, Camilla M Brena, Sarah J Evans, Muna F Anjum, Christopher J Teale
AIMS: In 2015, colistin-resistant Escherichia coli and Salmonella with the mcr-1 gene were isolated from a pig farm in Great Britain. Pigs were subsequently monitored over a ~ 20 month period for the occurrence of mcr-1 mediated colistin resistance and the risk of mcr-1 E. coli entering the food chain was assessed. METHODS AND RESULTS: Pig faeces and slurry were cultured for colistin resistant E. coli and Salmonella, tested for the mcr-1 gene by PCR, and selected isolates were further analysed...
May 9, 2018: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/29731740/evidence-for-environmental-dissemination-of-antibiotic-resistance-mediated-by-wild-birds
#10
Jiao Wu, Ye Huang, Dawei Rao, Yongkui Zhang, Kun Yang
The aquatic bird, egret, could carry antibiotic resistance (AR) from a contaminated waterway (Jin River, Chengdu, China) into the surrounding environment (Wangjianglou Park). A systematic study was carried out on the unique environmental dissemination mode of AR mediated by birds. The minimum inhibitory concentrations of various antibiotics against the environmental Escherichia coli isolates were used to evaluate the bacterial AR at the environmental locations where these isolates were recovered, i.e., the Jin River water, the egret feces, the park soil, and the campus soil...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29718423/efflux-pump-inhibitor-cccp-to-rescue-colistin-susceptibility-in-mcr-1-plasmid-mediated-colistin-resistant-strains-and-gram-negative-bacteria
#11
Sophie A Baron, Jean-Marc Rolain
Objectives: Efflux in bacteria is a ubiquitous mechanism associated with resistance to antimicrobials agents. Efflux pump inhibitors (EPIs) have been developed to inhibit efflux mechanisms and could be a good alternative to reverse colistin resistance, but only CCCP has shown good activity. The aim of our study was to identify CCCP activity in a collection of 93 Gram-negative bacteria with known and unknown colistin resistance mechanisms including isolates with mcr-1 plasmid-mediated colistin resistance...
April 26, 2018: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/29718144/genomic-surveillance-reveals-diversity-of-multidrug-resistant-organism-colonization-and-infection-a-prospective-cohort-study-in-liver-transplant-recipients
#12
Nenad Macesic, Angela Gomez-Simmonds, Sean B Sullivan, Marla J Giddins, Samantha A Ferguson, Gautam Korakavi, David Leeds, Sarah Park, Kevin Shim, Madeleine G Sowash, Melanie Hofbauer, Ryan Finkel, Yue Hu, Jared West, Nora C Toussaint, William G Greendyke, Benjamin A Miko, Marcus R Pereira, Susan Whittier, Elizabeth C Verna, Anne-Catrin Uhlemann
Background: Multidrug-resistant organisms (MDROs) are an important cause of morbidity and mortality after solid organ transplantation. We aimed to characterize MDRO colonization dynamics and infection in liver transplant (LT) recipients through innovative use of active surveillance and whole-genome sequencing (WGS). Methods: We prospectively enrolled consecutive adult patients undergoing LT from March 2014 to March 2016. Fecal samples were collected at multiple timepoints from time of enrollment to 12 months posttransplant...
April 28, 2018: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
https://www.readbyqxmd.com/read/29712655/genetic-and-functional-characterization-of-an-mcr-3-like-producing-escherichia-coli-recovered-from-swine-brazil
#13
Nicolas Kieffer, Patrice Nordmann, Andrea Micke Moreno, Luisa Zanolli Moreno, Richard Chaby, Aude Breton, Pierre Tissières, Laurent Poirel
A collection of 126 pigs were screened for carriage of colistin-resistant Enterobacteriaceae in a farm in Minas Gerais, Brazil. Out of this collection, eigth colistin-resistant Escherichia coli isolates were recovered, including one from Minas Gerais State, producing a new MCR-3 variant (MCR-3.12). Analysis of the lipopolysaccharide revealed that MCR-3.12 had a similar function as MCR-1 and MCR-2 by adding a phosphoethanolamine group to the lipid A. Genetic analysis showed that the mcr-3.12 gene was carried by an IncA/C2 plasmid and was embedded in an original genetic environment...
April 30, 2018: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/29696722/the-first-human-report-of-mobile-colistin-resistance-gene-mcr-1-in-finland
#14
Kirsi Gröndahl-Yli-Hannuksela, Emilia Lönnqvist, Teemu Kallonen, Laura Lindholm, Jari Jalava, Kaisu Rantakokko-Jalava, Jaana Vuopio
Colistin resistance mediated by mobile mcr-1 gene has raised concern during the last years. After steep increase in mcr-1 reports, other mcr-gene variants (mcr-2 to mcr-5) have been revealed as well. In 2016, a clinical study was conducted on asymptomatic stool carriage of extended spectrum beta-lactamase (ESBL) producing Escherichia coli and Klebsiella pneumoniae among Finnish adults. All suspected ESBL producing bacterial isolates were first tested by phenotypic ESBL-confirmation methods, and then further analyzed with whole genome sequencing to identify the resistance genes...
May 2018: APMIS: Acta Pathologica, Microbiologica, et Immunologica Scandinavica
https://www.readbyqxmd.com/read/29688801/genomic-characterization-of-nonclonal-mcr-1-positive-multidrug-resistant-klebsiella-pneumoniae-from-clinical-samples-in-thailand
#15
Apichai Srijan, Katie R Margulieux, Sirigade Ruekit, Erik Snesrud, Rosslyn Maybank, Oralak Serichantalergs, Rosarin Kormanee, Prawet Sukhchat, Jossin Sriyabhaya, Mary Hinkle, John M Crawford, Patrick McGann, Brett E Swierczewski
Multidrug-resistant Klebsiella pneumoniae strains are one of the most prevalent causes of nosocomial infections and pose an increasingly dangerous public health threat. The lack of remaining treatment options has resulted in the utilization of older drug classes, including colistin. As a drug of last resort, the discovery of plasmid-mediated colistin resistance by mcr-1 denotes the potential development of pandrug-resistant bacterial pathogens. To address the emergence of the mcr-1 gene, 118 gram-negative Enterobacteriaceae isolated from clinical samples collected at Queen Sirikit Naval Hospital in Chonburi, Thailand were screened for colistin resistance using automated antimicrobial susceptibility testing and conventional PCR screening...
April 24, 2018: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/29686149/retained-activity-of-an-o25b-specific-monoclonal-antibody-against-mcr-1-producing-escherichia-coli-st131
#16
Luis M Guachalla, Katharina Ramoni, Cecilia Varga, Michele Mutti, Akela Ghazawi, Tibor Pál, Eszter Nagy, Ágnes Sonnevend, Gábor Nagy, Valéria Szijártó
Plasmid-encoded colistin resistance is emerging among extra-intestinal pathogenic Escherichia coli including the epidemic clone ST131-H30. Mcr-1 transfers a phosphoethanolamine to the lipid A portion of LPS conferring resistance to polymyxins. We investigated whether this modification changed the activity of the monoclonal antibody, ASN-4 specific to the O25b side-chain of ST131 LPS. We confirmed that, unlike colistin, ASN-4 retained its bactericidal and endotoxin neutralizing activity and therefore offer a treatment option against extremely drug-resistant ST131 isolates...
April 23, 2018: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/29684127/the-presence-of-colisitn-resistance-gene-mcr-1-and-3-in-esbl-producing-escherichia-coli-isolated-from-food-in-ho-chi-minh-city-vietnam
#17
Takahiro Yamaguchi, Ryuji Kawahara, Kazuo Harada, Shihono Teruya, Tatsuya Nakayama, Daisuke Motooka, Shota Nakamura, Phuc Do Nguyen, Yuko Kumeda, Chinh Van Dang, Kazumasa Hirata, Yoshimasa Yamamoto
Colistin is indicated for the treatment of multidrug-resistant gram-negative bacterial infections. However, the spread of colistin-resistant bacteria harboring a mcr gene has become a serious concern. This study investigated local foods in Vietnam for contamination with colistin-resistant bacteria. A total of 261 extended-spectrum β-lactamase (ESBL)- and AmpC-producing Escherichia coli isolates from 330 meat and seafood products were analyzed for colistin susceptibility and the presence of mcr genes. Approximately 24% (62/261) of ESBL- or AmpC-producing E...
April 19, 2018: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/29667494/discovery-of-a-mcr-1-bearing-plasmid-in-commensal-colistin-resistant-escherichia-coli-from-healthy-broilers-in-faisalabad-pakistan
#18
Jiali Lv, Mashkoor Mohsin, Sheng Lei, Swaminath Srinivas, Raja Talish Wiqar, Jingxia Lin, Youjun Feng
No abstract text is available yet for this article.
April 18, 2018: Virulence
https://www.readbyqxmd.com/read/29665883/plasmid-mediated-colistin-resistance-in-animals-current-status-and-future-directions
#19
Jian Sun, Ximin Zeng, Xing-Ping Li, Xiao-Ping Liao, Ya-Hong Liu, Jun Lin
Colistin, a peptide antibiotic belonging to the polymyxin family, is one of the last effective drugs for the treatment of multidrug resistant Gram-negative infections. Recent discovery of a novel mobile colistin resistance gene, mcr-1, from people and food animals has caused a significant public health concern and drawn worldwide attention. Extensive usage of colistin in food animals has been proposed as a major driving force for the emergence and transmission of mcr-1; thus, there is a worldwide trend to limit colistin usage in animal production...
December 2017: Animal Health Research Reviews
https://www.readbyqxmd.com/read/29661868/spread-of-clonally-related-escherichia-coli-harboring-an-inca-c-1-plasmid-encoding-imp-8-and-its-recruitment-into-an-unrelated-mcr-1-containing-isolate
#20
Alan Elena, Daniela Cejas, Francisco Magariños, Virginia Jewtuchowicz, Andrea Facente, Gabriel Gutkind, José Di Conza, Marcela Radice
Ten IMP-8-producing Escherichia coli isolates were recovered from the surveillance cultures of a neonatal intensive care unit, of which eight were clonally related. A 168.2-kb- bla IMP-8 plasmid was fully sequenced, and it corresponded to the recently described IncA/C1-ST13. This plasmid was detected in all isolates, even in those no clonally related. One unrelated isolate was also resistant to colistin and positive for mcr-1. This marker was located in a 62.7-kb-IncI2 plasmid, which was also fully sequenced...
April 16, 2018: Antimicrobial Agents and Chemotherapy
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