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https://www.readbyqxmd.com/read/28098547/mcr-1-harboring-salmonella-enterica-serovar-typhimurium-sequence-type-34-in-pigs-china
#1
Linxian Yi, Jing Wang, Yanling Gao, Yiyun Liu, Yohei Doi, Renjie Wu, Zhenling Zeng, Zisen Liang, Jian-Hua Liu
We detected the mcr-1 gene in 21 (14.8%) Salmonella isolates from pigs at slaughter; 19 were serovar Typhimurium sequence type 34. The gene was located on IncHI2-like plasmids that also harbored IncF replicons and lacked a conjugative transfer region. These findings highlight the need to prevent further spread of colistin resistance in animals and humans.
February 2017: Emerging Infectious Diseases
https://www.readbyqxmd.com/read/28073965/spread-of-mcr-1-carrying-enterobacteriaceae-in-sewage-water-from-spain
#2
C M Ovejero, J F Delgado-Blas, W Calero-Caceres, M Muniesa, B Gonzalez-Zorn
OBJECTIVES: The mobile colistin resistance gene mcr-1 has been identified worldwide in human and animal sources, while its occurrence in the environment is still largely unknown. The aim of this study was to investigate the presence of mcr-1-harbouring Enterobacteriaceae in water samples obtained from rivers and waste water treatment plants in the area of Barcelona, Spain. METHODS: The presence of mcr-1 was detected by PCR. Bacterial identification was performed via MALDI-TOF MS...
January 10, 2017: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/28073961/genetic-characterization-of-mcr-1-bearing-plasmids-to-depict-molecular-mechanisms-underlying-dissemination-of-the-colistin-resistance-determinant
#3
Ruichao Li, Miaomiao Xie, Jinfei Zhang, Zhiqiang Yang, Lizhang Liu, Xiaobo Liu, Zhiwei Zheng, Edward Wai-Chi Chan, Sheng Chen
OBJECTIVES: To analyse and compare mcr-1-bearing plasmids from animal Escherichia coli isolates, and to investigate potential mechanisms underlying dissemination of mcr-1. METHODS: Ninety-seven ESBL-producing E. coli strains isolated from pig farms in China were screened for the mcr-1 gene. Fifteen mcr-1-positive strains were subjected to molecular characterization and bioinformatic analysis of the mcr-1-bearing plasmids that they harboured. RESULTS: Three major types of mcr-1-bearing plasmids were recovered: IncX4 (∼33 kb), IncI2 (∼60 kb) and IncHI2 (∼216-280 kb), among which the IncX4 and IncI2 plasmids were found to harbour the mcr-1 gene only, whereas multiple resistance elements including blaCTX-M, blaCMY, blaTEM, fosA, qnrS, floR and oqxAB were detected, in various combinations, alongside mcr-1 in the IncHI2 plasmids...
September 28, 2016: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/28069644/a-high-incidence-of-escherichia-coli-strains-co-harboring-mcr-1-and-blandm-from-chickens
#4
Bao-Tao Liu, Feng-Jing Song, Ming Zou, Qi-Di Zhang, Hu Shan
This study investigated the characteristics of E. coli isolates carrying mcr-1-blaNDM from a chicken farm in China. Of the 78 E. coli isolates, 21 clonally unrelated isolates carried mcr-1-blaNDM Diverse IncI2 plasmids disseminated mcr-1, while the dissemination of blaNDM was mediated by diverse IncB/O plasmids. More striking is the co-localization of resistance genes mcr-1 and blaNDM-4 on an IncHI2/ST3 plasmid and this might pose a great challenge for public health.
January 9, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28059088/insights-into-the-mechanistic-basis-of-plasmid-mediated-colistin-resistance-from-crystal-structures-of-the-catalytic-domain-of-mcr-1
#5
Philip Hinchliffe, Qiu E Yang, Edward Portal, Tom Young, Hui Li, Catherine L Tooke, Maria J Carvalho, Neil G Paterson, Jürgen Brem, Pannika R Niumsup, Uttapoln Tansawai, Lei Lei, Mei Li, Zhangqi Shen, Yang Wang, Christopher J Schofield, Adrian J Mulholland, Jianzhong Shen, Natalie Fey, Timothy R Walsh, James Spencer
The polymixin colistin is a "last line" antibiotic against extensively-resistant Gram-negative bacteria. Recently, the mcr-1 gene was identified as a plasmid-mediated resistance mechanism in human and animal Enterobacteriaceae, with a wide geographical distribution and many producer strains resistant to multiple other antibiotics. mcr-1 encodes a membrane-bound enzyme catalysing phosphoethanolamine transfer onto bacterial lipid A. Here we present crystal structures revealing the MCR-1 periplasmic, catalytic domain to be a zinc metalloprotein with an alkaline phosphatase/sulphatase fold containing three disulphide bonds...
January 6, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28056227/detection-and-dissemination-of-the-colistin-resistance-gene-mcr-1-from-isolates-and-fecal-samples-in-china
#6
Xia Chen, Xiaofei Zhao, Jie Che, Yanwen Xiong, Yanmei Xu, Lifeng Zhang, Ruiting Lan, Lining Xia, Timothy R Walsh, Jianguo Xu, Jinxing Lu, Juan Li
A recently identified colistin resistance gene, mcr-1, has been reported in many countries. In this study, we established a new real-time PCR method to detect the mcr-1 gene in a variety of isolates and fecal samples from 20 provinces and municipal cities in China. Of the 2330 isolates (from 10 species) screened, 54 (2.3%) isolates were positive for mcr-1. All of the mcr-1-positive isolates that were identified belonged to Escherichia coli (E. coli) strains, among which 9, 1, and 44 were identified as enteropathogenic E...
January 5, 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28031275/mcr-1-in-esbl-producing-escherichia-coli-responsible-for-human-infections-in-new-caledonia
#7
Frederic Robin, Racha Beyrouthy, Julien Colot, Pierre Saint-Sardos, Anne Berger-Carbonne, Guillaume Dalmasso, Julien Delmas, Richard Bonnet
No abstract text is available yet for this article.
December 28, 2016: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/28031274/escherichia-coli-carrying-incx4-plasmid-mediated-mcr-1-and-blactx-m-genes-in-infected-migratory-magellanic-penguins-spheniscus-magellanicus
#8
Fábio P Sellera, Miriam R Fernandes, Luciana Sartori, Marcelo P N Carvalho, Fernanda Esposito, Cristiane L Nascimento, Gustavo H P Dutra, Elsa M Mamizuka, Paula J Pérez-Chaparro, John A McCulloch, Nilton Lincopan
No abstract text is available yet for this article.
December 28, 2016: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/28031273/complete-genome-sequencing-revealed-novel-genetic-contexts-of-the-mcr-1-gene-in-escherichia-coli-strains
#9
Choo Yee Yu, Geik Yong Ang, Teik-Min Chong, Pui San Chin, Yun Fong Ngeow, Wai-Fong Yin, Kok-Gan Chan
No abstract text is available yet for this article.
December 28, 2016: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/28031198/a-phage-like-incy-plasmid-carrying-the-mcr-1-gene-in-escherichia-coli-from-a-pig-farm-in-china
#10
Chunping Zhang, Yuqing Feng, Fei Liu, Hui Jiang, Zhina Qu, Meng Lei, Jianfeng Wang, Bing Zhang, Yongfei Hu, Jiabo Ding, Baoli Zhu
We report here a new type of plasmid that carries the mcr-1 gene, the pMCR-1-P3 plasmid, harbored in an Escherichia coli strain isolated from a pig farm in China. pMCR-1-P3 belongs to the IncY incompatibility group, and is a phage-like plasmid that contains a large portion of phage-related sequences. The backbone of this plasmid is different from other mcr-1-carrying plasmids reported previously.
December 28, 2016: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28018876/frequency-of-mcr-1-mediated-colistin-resistance-among-escherichia-coli-clinical-isolates-obtained-from-patients-in-canadian-hospitals-canward-2008-2015
#11
Andrew Walkty, James A Karlowsky, Heather J Adam, Philippe Lagacé-Wiens, Melanie Baxter, Michael R Mulvey, Melissa McCracken, Susan M Poutanen, Diane Roscoe, George G Zhanel
BACKGROUND: Colistin is often used as an antimicrobial of last resort for the treatment of infections caused by multidrug-resistant gram-negative bacilli. In 2015, plasmid-mediated colistin resistance in Escherichia coli due to MCR-1 was described. The purpose of this study was to evaluate the frequency of colistin resistance among E. coli clinical isolates obtained from patients in Canadian hospitals as part of the Canadian Ward Surveillance Study (CANWARD) and to determine how often the mcr-1 gene is detected among the colistin-resistant subset...
October 2016: CMAJ Open
https://www.readbyqxmd.com/read/28000749/high-resolution-crystal-structure-of-the-catalytic-domain-of-mcr-1
#12
Guixing Ma, Yifan Zhu, Zhicheng Yu, Ashfaq Ahmad, Hongmin Zhang
The newly identified mobile colistin resistant gene (mcr-1) rapidly spread among different bacterial strains and confers colistin resistance to its host, which has become a global concern. Based on sequence alignment, MCR-1 should be a phosphoethanolamine transferase, members of the YhjW/YjdB/YijP superfamily and catalyze the addition of phosphoethanolamine to lipid A, which needs to be validated experimentally. Here we report the first high-resolution crystal structure of the C-terminal catalytic domain of MCR-1 (MCR-1C) in its native state...
December 21, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27999050/complete-genetic-analysis-of-plasmids-carrying-mcr-1-and-other-resistance-genes-in-an-escherichia-coli-isolate-of-animal-origin
#13
Ruichao Li, Miaomiao Xie, Jingzhang Lv, Edward Wai-Chi Chan, Sheng Chen
OBJECTIVES: To investigate the genetic features of three plasmids recovered from an MCR-1 and ESBL-producing Escherichia coli strain, HYEC7, and characterize the transmission mechanism of mcr-1 METHODS: The genetic profiles of three plasmids were determined by PCR, S1-PFGE, Southern hybridization and WGS analysis. The ability of the mcr-1-bearing plasmid to undergo conjugation was also assessed. The mcr-1-bearing transposon Tn6330 was characterized by PCR and DNA sequencing. RESULTS: Complete sequences of three plasmids were obtained...
December 20, 2016: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/27999032/occurrence-and-characterization-of-mcr-1-harbouring-escherichia-coli-isolated-from-pigs-in-great-britain-from-2013-to-2015
#14
Nicholas A Duggett, Ellie Sayers, Manal AbuOun, Richard J Ellis, Javier Nunez-Garcia, Luke Randall, Robert Horton, Jon Rogers, Francesca Martelli, Richard P Smith, Camilla Brena, Susanna Williamson, Miranda Kirchner, Robert Davies, Derrick Crook, Sarah Evans, Chris Teale, Muna F Anjum
OBJECTIVES: To determine the occurrence of mcr-1-harbouring Escherichia coli in archived pig material originating in Great Britain (GB) from 2013 to 2015 and characterize mcr-1 plasmids. METHODS: Enrichment and selective culture of 387 archived porcine caecal contents and recovery from archive of 1109 E. coli isolates to identify colistin-resistant bacteria by testing for the presence of mcr-1 by PCR and RT-PCR. mcr-1-harbouring E. coli were characterized by WGS and compared with other available mcr-1 WGS...
December 20, 2016: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/27993847/various-sequence-types-of-escherichia-coli-co-harboring-blandm-5-and-mcr-1-genes-from-a-commercial-swine-farm-in-china
#15
Ling-Han Kong, Chang-Wei Lei, Su-Zhen Ma, Wei Jiang, Bi-Hui Liu, Yong-Xiang Wang, Ru Guan, Shuai Men, Qi-Wu Yuan, Guang-Yang Cheng, Wen-Cheng Zhou, Hong-Ning Wang
Sixteen different sequence types of Escherichia coli isolates from a commercial swine farm in China were confirmed co-harboring carbapenems resistance gene blaNDM-5 and colistin resistance gene mcr-1 Whole genome sequencing revealed that blaNDM-5 and mcr-1 were located on a 46-kb IncX3 plasmid and a 32-kb IncX4 plasmid, respectively. The two plasmids could transfer together with low fitness cost, which might be responsible for the presence of various STs of E. coli co-harboring blaNDM-5 and mcr-1.
December 19, 2016: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/27958270/crystal-structure-of-escherichia-coli-originated-mcr-1-a-phosphoethanolamine-transferase-for-colistin-resistance
#16
Menglong Hu, Jiubiao Guo, Qipeng Cheng, Zhiqiang Yang, Edward Wai Chi Chan, Sheng Chen, Quan Hao
MCR-1 is a phosphoethanolamine (pEtN) transferase that modifies the pEtN moiety of lipid A, conferring resistance to colistin, which is an antibiotic belonging to the class of polypeptide antibiotics known as polymyxins and is the last-line antibiotic used to treat multidrug resistant bacterial infections. Here we determined the crystal structure of the catalytic domain of MCR-1 (MCR-1-ED), which is originated in Escherichia coli (E. coli). MCR-1-ED was found to comprise several classical β-α-β-α motifs that constitute a "sandwich" conformation...
December 13, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27940944/colistin-resistance-in-carbapenem-resistant-klebsiella-pneumoniae-laboratory-detection-and-impact-on-mortality
#17
Laura J Rojas, Madiha Salim, Eric Cober, Sandra S Richter, Federico Perez, Robert A Salata, Robert C Kalayjian, Richard R Watkins, Steve Marshall, Susan D Rudin, T Nicholas Domitrovic, Andrea M Hujer, Kristine M Hujer, Yohei Doi, Keith S Kaye, Scott Evans, Vance G Fowler, Robert A Bonomo, David van Duin
BACKGROUND: Polymyxins including colistin are an important "last-line" treatment for infections caused by carbapenem-resistant Klebsiella pneumoniae (CRKp). Increasing use of colistin has led to resistance to this cationic antimicrobial peptide. METHODS: A cohort nested within the Consortium on Resistance against Carbapenems in Klebsiella pneumoniae (CRACKLE) was constructed of patients with infection, or colonization with CRKp isolates tested for colistin susceptibility during the study period of December, 2011 to October, 2014...
December 10, 2016: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
https://www.readbyqxmd.com/read/27917926/clonal-spread-of-mcr-1-in-pmqr-carrying-st34-salmonella-isolates-from-animals-in-china
#18
Xing-Ping Li, Liang-Xing Fang, Jia-Qi Song, Jing Xia, Wei Huo, Jin-Tao Fang, Xiao-Ping Liao, Ya-Hong Liu, Youjun Feng, Jian Sun
Since initial identification in China, the widespread geographical occurrence of plasmid-mediated colistin resistance gene mcr-1 in Enterobacteriaceae has been of great concern. In this study, a total of 22 Salmonella enterica were resistant to colistin, while only five isolates which belonged to ST34 Salmonella enterica serovar Typhimurium (S. Typhimurium) were mcr-1 positive. Four of them shared nearly identical PFGE type, although they were from different host species and diverse geographical locations. All the mcr-1-positive S...
December 5, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27915108/how-should-we-respond-to-the-emergence-of-plasmid-mediated-colistin-resistance-in-humans-and-animals
#19
REVIEW
Jaffar A Al-Tawfiq, Ramanan Laxminarayan, Marc Mendelson
OBJECTIVE: The widespread use of antibiotics in humans and animals has contributed to growing rates of antibiotic resistance. Previously treatable bacterial infections now require the last line of antibiotics or are untreatable. The current antibiotic of last resort for carbapenem-resistant Gram-negative bacterial infections is often colistin. Evidence for the shifting pattern of colistin resistance and how the international community should respond are discussed in this review. METHODS: The literature on colistin resistance was reviewed...
January 2017: International Journal of Infectious Diseases: IJID
https://www.readbyqxmd.com/read/27895009/an-incp-plasmid-carrying-the-colistin-resistance-gene-mcr-1-in-klebsiella-pneumoniae-from-hospital-sewage
#20
Feifei Zhao, Yu Feng, Xiaoju Lü, Alan McNally, Zhiyong Zong
A Klebsiella pneumoniae strain of ST313 recovered from hospital sewage was found to carry the plasmid-borne colistin resistance gene mcr-1, which was bracketed by two copies of the insertion sequence ISApl1 on a 57-kb self-transmissible IncP type plasmid of a new IncP-1 clade. The carriage of mcr-1 on a self-transmissible broad-host-range plasmid highlights that mcr-1 has the potential to be spread beyond the Enterobacteriaceae.
November 28, 2016: Antimicrobial Agents and Chemotherapy
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