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https://www.readbyqxmd.com/read/28919348/prevalence-of-colistin-resistance-in-clinical-isolates-of-enterobacteriaceae-a-four-year-cross-sectional-study
#1
Núria Prim, Miquel Turbau, Alba Rivera, Judith Rodríguez-Navarro, Pere Coll, Beatriz Mirelis
Objectives The objectives were to determine the prevalence of colistin resistance in clinical isolates of Enterobacteriaceae, and to gain knowledge on the epidemiological and clinical features of the patients. Methods All colistin-resistant Enterobacteriaceae consecutively isolated from clinical samples in our institution from 2012 to 2015, were included in this cross-sectional study. Intrinsic-resistant species with were excluded. Minimum inhibitory concentration was performed by gradient diffusion. Detection of plasmid-encoded colistin resistance genes mcr-1 and mcr-2 was performed by amplification...
September 14, 2017: Journal of Infection
https://www.readbyqxmd.com/read/28919044/susceptibility-of-colistin-resistant-pathogens-to-predatory-bacteria
#2
Sonal Dharani, Dong Hyun Kim, Robert M Q Shanks, Yohei Doi, Daniel E Kadouri
The increase in multidrug-resistant Gram-negative bacterial infections has forced the reintroduction of antibiotics such as colistin. However, the spread of plasmid-borne mcr-1 colistin resistance genes have moved us closer to an era of untreatable Gram-negative infections. To evaluate whether predatory bacteria could be used as a potential therapeutic to treat this upcoming threat, the ability of Bdellovibrio bacteriovorus and Micavibrio aeruginosavorus to prey on several clinically relevant mcr-1-positive, colistin-resistant isolates was evaluated...
September 14, 2017: Research in Microbiology
https://www.readbyqxmd.com/read/28912328/complete-genome-sequence-of-salmonella-enterica-subsp-enterica-serovar-paratyphi-b-sequence-type-28-harboring-mcr-1
#3
Maria Borowiak, Jens A Hammerl, Jennie Fischer, Istvan Szabo, Burkhard Malorny
In 2015, plasmid-mediated colistin resistance was reported to be caused by a mobilized phosphoethanolamine transferase gene (mcr-1) in Enterobacteriaceae Here, we announce the complete genome sequence of the earliest d-tartrate-fermenting Salmonella enterica subsp. enterica serovar Paratyphi B isolate harboring mcr-1 from the collection of the German National Reference Laboratory for Salmonella.
September 14, 2017: Genome Announcements
https://www.readbyqxmd.com/read/28910166/prevalence-antimicrobial-resistance-and-relatedness-of-salmonella-isolated-from-chickens-and-pigs-on-farms-abattoirs-and-markets-in-sichuan-province-china
#4
Suzhen Ma, Changwei Lei, Linghan Kong, Wei Jiang, Bihui Liu, Shuai Men, Yanxian Yang, Guangyang Cheng, Yanpeng Chen, Hongning Wang
This study aims at investigating the distribution, antimicrobial resistance, and genetic relationship of Salmonella isolated from 18 farms, their downstream abattoirs, and markets of chickens and pigs in Sichuan province, China. A total of 193 Salmonella isolates were identified from 693 samples with an isolation rate of 26.27% (88/335) in chickens and 29.33% (105/358) in pigs. Salmonella was isolated more frequently in abattoirs and markets than from farms. Serotypes were determined according to the White-Kauffmann-Le Minor scheme and 16 different serotypes were identified, with Derby being the most common, followed by Typhimurium and Meleagridis...
September 14, 2017: Foodborne Pathogens and Disease
https://www.readbyqxmd.com/read/28904264/zoonotic-potential-and-antibiotic-resistance-of-escherichia-coli-in-neonatal-calves-in-uruguay
#5
Ana Umpiérrez, Inés Bado, Martín Oliver, Sofía Acquistapace, Analía Etcheverría, Nora Lía Padola, Rafael Vignoli, Pablo Zunino
Escherichia coli is one of the main etiological agents of neonatal calf diarrhea (NCD). The objective of this study was to assess the presence of virulence genes, genetic diversity, and antibiotic resistance mechanisms in E. coli associated with NCD in Uruguay. PCR was used to assess the presence of intimin, Shiga-like toxin, and stable and labile enterotoxin genes. Resistance to fluoroquinolones and oxyimino-cephalosporins was estimated on Müller-Hinton agar plates. Further antibiotic disc-diffusion tests were performed to assess bacterial multi-resistance...
September 12, 2017: Microbes and Environments
https://www.readbyqxmd.com/read/28903169/-a-rare-mechanism-of-resistance-to-colistin-in-escherichia-coli-isolated-from-raw-poultry-meat
#6
Renáta Karpíšková, Ivana Koláčková, Tereza Gelbíčová, Marie Zobaníková
Plasmid-mediated resistance to colistin is a recently described phenomenon. The study reports this new type of colistin resistance in food isolates of Escherichia coli in the Czech Republic. Strains with phenotypically determined colistin resistance were studied for presence of the mcr-1 and mcr-2 genes. A positive finding of E. coli harboring the mcr-1 gene was confirmed in a sample of raw minced turkey meat imported from Poland. Two different strains of E. coli carrying the mcr-1 gene were detected in the same sample...
June 2017: Klinická Mikrobiologie a Infekc̆ní Lékar̆ství
https://www.readbyqxmd.com/read/28893788/genomic-and-molecular-characterization-of-clinical-isolates-of-enterobacteriaceae-harboring-mcr-1-in-colombia-2002-2016
#7
Sandra Yamile Saavedra, Lorena Diaz, Magdalena Wiesner, Adriana Correa, Stefany Alejandra Arévalo, Jinnethe Reyes, Andrea Melissa Hidalgo, Elsa de la Cadena, Marcela Perenguez, Lucy Angeline Montaño, Javier Ardila, Rafael Ríos, María Victoria Ovalle, Paula Díaz, Paola Porras, Maria V Villegas, Cesar A Arias, Mauricio Beltrán, Carolina Duarte
Polymyxins are last resort antimicrobial agents used to treat infections caused by carbapenem-resistant Enterobacteriaceae Due to the worldwide dissemination of polymyxin resistance in animal and human isolates, we aimed to characterize polymyxin resistance associated with the presence of mcr-1 in Enterobacteriaceae and nonfermenter Gram-negative bacilli, in isolates collected retrospectively in Colombia from 2002 to 2016. A total of 5,887 Gram-negative clinical isolates were studied and 513 were found to be resistant to the polymyxins...
September 11, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28887199/temporal-evolution-of-polymyxin-b-resistant-klebsiella-pneumoniae-clones-recovered-from-blood-cultures-in-a-teaching-hospital-during-a-7-year-period
#8
Graziela Braun, Rodrigo Cayô, Adriana Pereira Matos, Juliane de Mello Fonseca, Ana Cristina Gales
The polymyxins have become one of the last resorts to treat serious infections caused by KPC-2-producing Klebsiella pneumoniae worldwide. However, the increase of polymyxin consumption has favored the emergence of resistance to these compounds. In this study we observed an increase in the polymyxin B resistance rates from 0 to 30.6% among 224 K. pneumoniae isolates recovered from blood cultures between 2009 and 2015. Only gentamicin, tigecycline and fosfomycin remained active against the polymyxin B-resistant K...
September 5, 2017: International Journal of Antimicrobial Agents
https://www.readbyqxmd.com/read/28878890/key-features-of-mcr-1-bearing-plasmids-from-escherichia-coli-isolated-from-humans-and-food
#9
Katrin Zurfluh, Magdalena Nüesch-Inderbinen, Jochen Klumpp, Laurent Poirel, Patrice Nordmann, Roger Stephan
BACKGROUND: Mcr-1-harboring Enterobacteriaceae are reported worldwide since their first discovery in 2015. However, a limited number of studies are available that compared full-length plasmid sequences of human and animal origins. METHODS: In this study, mcr-1-bearing plasmids from seven Escherichia coli isolates recovered from patients (n = 3), poultry meat (n = 2) and turkey meat (n = 2) in Switzerland were further analyzed and compared. Isolates were characterized by multilocus sequence typing (MLST)...
2017: Antimicrobial Resistance and Infection Control
https://www.readbyqxmd.com/read/28877757/whole-genome-sequencing-of-esbl-producing-escherichia-coli-isolated-from-patients-farm-waste-and-canals-in-thailand
#10
Chakkaphan Runcharoen, Kathy E Raven, Sandra Reuter, Teemu Kallonen, Suporn Paksanont, Jeeranan Thammachote, Suthatip Anun, Beth Blane, Julian Parkhill, Sharon J Peacock, Narisara Chantratita
BACKGROUND: Tackling multidrug-resistant Escherichia coli requires evidence from One Health studies that capture numerous potential reservoirs in circumscribed geographic areas. METHODS: We conducted a survey of extended β-lactamase (ESBL)-producing E. coli isolated from patients, canals and livestock wastewater in eastern Thailand between 2014 and 2015, and analyzed isolates using whole genome sequencing. RESULTS: The bacterial collection of 149 isolates consisted of 84 isolates from a single hospital and 65 from the hospital sewer, canals and farm wastewater within a 20 km radius...
September 6, 2017: Genome Medicine
https://www.readbyqxmd.com/read/28842282/detection-and-quantification-of-the-plasmid-mediated-mcr-1-gene-conferring-colistin-resistance-in-wastewater
#11
Itziar Lekunberri, José Luis Balcázar, Carles M Borrego
There is a global concern about the increasing resistance of bacterial pathogens to colistin, an antibiotic of last resort for the treatment of multidrug-resistant Gram-negative bacterial infections (e.g. carbapenemase-producing Enterobacteriaceae). In this study, we demonstrated an increasing prevalence of colistin resistance in raw and treated wastewater using a new developed SYBR Green-based real-time PCR assay for specific detection and quantification of the plasmid-mediated mcr-1 gene. Raw and treated wastewater samples were collected from a wastewater treatment plant in Girona (Spain) at two different time periods (November 2011-January 2012 and December 2016-February 2017)...
August 22, 2017: International Journal of Antimicrobial Agents
https://www.readbyqxmd.com/read/28838854/colistin-resistance-among-blood-culture-isolates-at-a-tertiary-care-centre-in-hungary
#12
Emese Juhász, Miklós Iván, Eszter Pintér, Júlia Pongrácz, Katalin Kristóf
OBJECTIVES: The emergence of colistin resistance has been detected worldwide in recent years. While colistin susceptibility has been tested in carbapenem resistant Enterobacteriaceae, MDR Pseudomonas spp. and Acinetobacter spp. during routine laboratory practice, the overall rate of colistin resistance was unknown in our centre. The aim of this retrospective study was to reveal the prevalence of colistin resistance among clinically significant blood culture isolates in two different periods (2010-2011 and 2016) in our laboratory...
August 21, 2017: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/28828173/high-prevalence-of-the-mcr-1-gene-in-retail-chicken-meat-in-the-netherlands-in-2015
#13
Eefje J A Schrauwen, Pepijn Huizinga, Nick van Spreuwel, Carlo Verhulst, Marjolein F Q Kluytmans-van den Bergh, Jan A J W Kluytmans
Recently, plasmid-mediated colistin resistance was reported in humans, animals and food. We studied the presence of mcr-1 and mcr-2 in Dutch retail chicken meat. The prevalence of mcr-1 was 24,8% (53/214), whereas mcr-2 was not found. The presence of mcr-1-positive Enterobacteriaceae was confirmed by culture in 34/53 samples (64,2%). The prevalence depended on the supermarket chain and was lower in free-range chicken samples. The unexpected high prevalence of mcr-1 in food is cause for concern.
2017: Antimicrobial Resistance and Infection Control
https://www.readbyqxmd.com/read/28827120/a-simple-phenotypic-method-for-screening-of-mcr-1-mediated-colistin-resistance
#14
Marco Coppi, Antonio Cannatelli, Alberto Antonelli, Ilaria Baccani, Vincenzo Di Pilato, Samanta Sennati, Tommaso Giani, Gian Maria Rossolini
OBJECTIVES: To evaluate a novel method, named Colistin-MAC test, for phenotypic screening of acquired colistin-resistance mediated by transferable mcr-1 resistance determinants, based on colistin MIC reduction in the presence of dipicolinic acid (DPA). METHODS: The Colistin-MAC test consists in a broth microdilution method, in which colistin MIC is tested in absence or presence of DPA (900 μg/mL). Overall, 74 colistin-resistant strains of Enterobacteriaceae (65 Escherichia coli and 9 of other species), including 61 strains carrying mcr-1-like genes and 13 strains negative for mcr genes, were evaluated with the Colistin-MAC test...
August 18, 2017: Clinical Microbiology and Infection
https://www.readbyqxmd.com/read/28799833/plasmid-mediated-colistin-resistance-gene-mcr-1-in-an-escherichia-coli-st10-bloodstream-isolate-in-the-sultanate-of-oman
#15
Jalila Mohsin, Tibor Pál, Jorgen Eskild Petersen, Dania Darwish, Akela Ghazawi, Tanveer Ashraf, Agnes Sonnevend
AIMS: To identify plasmid-mediated colistin resistance in clinical Enterobacteriaceae isolates in Oman, where this resistance mechanism has not been encountered yet. MATERIALS/METHODS: Twenty-two colistin-resistant Enterobacteriaceae clinical isolates collected between July 2014 and June 2016 in a tertiary care hospital in Muscat were screened by PCR for the mcr-1 and mcr-2 genes. The strain identified as mcr-1 positive was genotyped and its antibiotic susceptibility was established...
August 11, 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/28797328/co-occurrence-of-colistin-resistance-genes-mcr-1-and-mcr-3-among-multidrug-resistant-escherichia-coli-isolated-from-cattle-spain-september-2015
#16
Marta Hernández, M Rocío Iglesias, David Rodríguez-Lázaro, Alejandro Gallardo, Narciso Quijada, Pedro Miguela-Villoldo, Maria Jorge Campos, Segundo Píriz, Gema López-Orozco, Cristina de Frutos, José Luis Sáez, María Ugarte-Ruiz, Lucas Domínguez, Alberto Quesada
Colistin resistance genes mcr-3 and mcr-1 have been detected in an Escherichia coli isolate from cattle faeces in a Spanish slaughterhouse in 2015. The sequences of both genes hybridised to same plasmid band of ca 250 kb, although colistin resistance was non-mobilisable. The isolate was producing extended-spectrum beta-lactamases and belonged to serotype O9:H10 and sequence type ST533. Here we report an mcr-3 gene detected in Europe following earlier reports from Asia and the United States.
August 3, 2017: Euro Surveillance: Bulletin Européen sur les Maladies Transmissibles, European Communicable Disease Bulletin
https://www.readbyqxmd.com/read/28797325/plasmid-borne-colistin-resistance-gene-mcr-3-in-salmonella-isolates-from-human-infections-denmark-2009-17
#17
Eva Litrup, Kristoffer Kiil, Anette M Hammerum, Louise Roer, Eva M Nielsen, Mia Torpdahl
This report describes one Salmonella isolate harbouring both mcr-1 and mcr-3. We also found nine other Salmonella isolates positive for the plasmid-borne colistin resistance gene, mcr-3. The strains were isolated from patients in Denmark between 2009 and 2017 and five of the patients had travelled to Asia. In addition to mcr-3, all strains were found positive for blaTEM-1, strA, strB, sul2 and tet(A) or tet(B), and most strains were positive for blaCTX-M-55 and qnrS.
August 3, 2017: Euro Surveillance: Bulletin Européen sur les Maladies Transmissibles, European Communicable Disease Bulletin
https://www.readbyqxmd.com/read/28782705/investigation-of-potential-risk-factors-for-the-occurrence-of-escherichia-coli-isolates-from-german-fattening-pig-farms-harbouring-the-mcr-1-colistin-resistance-gene
#18
Katja Hille, Nicole Roschanski, Inga Ruddat, Johanna Woydt, Maria Hartmann, Uwe Rösler, Lothar Kreienbrock
Within the scope of a cross-sectional investigation on fattening pig farms conducted in 2011 and 2012, 48 fattening farms in different agricultural regions of Germany were studied. Primary cultures of boot swabs and collective faecal samples were stored at -80°C and screened for the presence of the mcr-1 colistin resistance gene. The laboratory results were linked to farm related data collected via questionnaire. We used logistic regression models to investigate the association between occurrence of mcr-1 and farm related data...
August 3, 2017: International Journal of Antimicrobial Agents
https://www.readbyqxmd.com/read/28780361/untreated-urban-waste-contaminates-indian-river-sediments-with-resistance-genes-to-last-resort-antibiotics
#19
Nachiket P Marathe, Chandan Pal, Swapnil S Gaikwad, Viktor Jonsson, Erik Kristiansson, D G Joakim Larsson
Efficient sewage treatment is critical for limiting environmental transmission of antibiotic-resistant bacteria. In many low and middle income countries, however, large proportions of sewage are still released untreated into receiving water bodies. In-depth knowledge of how such discharges of untreated urban waste influences the environmental resistome is largely lacking. Here, we highlight the impact of uncontrolled discharge of partially treated and/or untreated wastewater on the structure of bacterial communities and resistome of sediments collected from Mutha river flowing through Pune city in India...
July 25, 2017: Water Research
https://www.readbyqxmd.com/read/28780246/detection-and-genetic-features-of-mcr-1-producing-plasmid-in-human-escherichia-coli-infection-in-south-korea
#20
Eun Sil Kim, Yong Pil Chong, Su-Jin Park, Mi-Na Kim, Sung-Han Kim, Sang-Oh Lee, Sang-Ho Choi, Jun Hee Woo, Jin-Yong Jeong, Yang Soo Kim
The plasmid-mediated colistin resistance gene, mcr-1, was identified for the first time from a hospitalized patient in South Korea. The mcr-1 gene was successfully transferred to E. coli J53 recipient and conferred resistance to colistin in the recipient. The mcr-1-harboring plasmid possessed a typical IncI2 group and did not have the mcr-1-associated ISApl1 element.
July 2, 2017: Diagnostic Microbiology and Infectious Disease
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