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https://www.readbyqxmd.com/read/28927144/mir-381-inhibits-migration-and-invasion-in-human-gastric-carcinoma-through-downregulatedting-sox4
#1
Mingming Zhang, Shishu Huang, Dan Long
Aberrant expression of microRNAs (miRs) serves essential roles in the generation and progression of various types of human cancer. In the present study, the expression and biological functions of miR-381 in human gastric carcinoma (GC) were focused upon. The results of reverse transcription-quantitative polymerase chain reaction analysis revealed that the expression of miR-381 was significantly downregulated in GC tissue samples. Furthermore, low expression of miR-381 was identified to be associated with lymphatic metastasis and advanced tumor-node-metastasis stage (III+IV)...
September 2017: Oncology Letters
https://www.readbyqxmd.com/read/28927076/microrna-154-inhibits-the-growth-and-metastasis-of-gastric-cancer-cells-by-directly-targeting-mtdh
#2
Wenhui Qiao, Nong Cao, Lei Yang
MicroRNAs (miRNAs) are a group of non-protein-coding, highly conserved single-stranded RNA molecules. The abnormal expression of miRNAs has been demonstrated to have an important function in the carcinogenesis and progression of gastric cancer. microRNA-154 (miR-154) has been reported to be downregulated in non-small cell lung, colorectal and prostate cancer. However, the expression and roles of miR-154 in gastric cancer remain to be established. The present study measured the expression levels of miR-154 in gastric cancer tissues and cell lines...
September 2017: Oncology Letters
https://www.readbyqxmd.com/read/28927055/silencing-dna-methyltransferase-1-leads-to-the-activation-of-the-esophageal-suppressor-gene-p16-in-vitro-and-in-vivo
#3
Jian Bai, Xue Zhang, Bangqing Liu, Haiyong Wang, Zhenzong Du, Jianfei Song
Previous studies have demonstrated that DNA methyltransferase 1 (DNMT1) is required for the maintenance of DNA methylation and epigenetic changes that may lead to the development of esophageal squamous cell carcinoma (ESCC). In order to investigate whether the silencing of DNMT1 protects tumor suppressor genes, including p16, and is able to be used as a potential therapy for human ESCC, short hairpin RNA targeting DNMT1 (shRNA-DNMT1) was synthesized and transfected into the human ESCC lines KYSE150 and KYSE410, which were then injected into the backs of nude mice prior to harvesting...
September 2017: Oncology Letters
https://www.readbyqxmd.com/read/28927029/differences-in-zbtb7a-expression-cause-heterogeneous-changes-in-human-nasopharyngeal-carcinoma-cne3-sublines
#4
Fei Liu, Jiao Lan, Wei Jiao, Xianglan Mo, Yongta Huang, Huilan Ye, Ruiping Xiao, Yongli Wang, Mingzheng Mo, Liwei Shi
The present study aimed to determine the association between changes in Zbtb7a expression levels and heterogeneity of nasopharyngeal carcinoma (NPC) CNE3 sublines. CNE3 sublines were established by screening of serial dilution and continuous passage. Proliferative ability and tumorigenicity of the sublines were analyzed separately by soft-agar colony formation and mouse studies. The NPC tissues from mice were analyzed by histological evaluation and immunohistochemistry. The expression levels of Zbtb7a mRNA and protein were analyzed separately by quantitative reverse transcription polymerase chain reaction and western blotting...
September 2017: Oncology Letters
https://www.readbyqxmd.com/read/28927028/decreased-expression-of-the-long-non-coding-rna-mllt4-antisense-rna-1-is-a-potential-biomarker-and-an-indicator-of-a-poor-prognosis-for-gastric-cancer
#5
Yuexing Lai, Ping Xu, Jie Liu, Qinghua Li, Dabin Ren, Jun Zhang, Jing Wang
In recent years, the identification of long non-coding RNAs (lncRNAs) led to the analysis of their characteristics in cancer biology. However, the expression of lncRNAs in cancer and their clinical significance remain unclear. In the present study, an investigation of lncRNAs that may be involved in the regulation of metastasis using microarray and polymerase chain reaction analyses resulted in the identification of MLLT4 antisense RNA 1 (MLLT4-AS1) as a significantly downregulated lncRNA in gastric cancer tissue compared with normal adjacent tissue (P=0...
September 2017: Oncology Letters
https://www.readbyqxmd.com/read/28925811/it-s-fun-to-transcribe-with-fun30-a-model-for-nucleosome-dynamics-during-rna-polymerase-ii-mediated-elongation
#6
Junwoo Lee, Eun Shik Choi, Daeyoup Lee
The ability of elongating RNA polymerase II (RNAPII) to regulate the nucleosome barrier is poorly understood because we do not know enough about the involved factors and we lack a conceptual framework to model this process. Our group recently identified the conserved Fun30/SMARCAD1 family chromatin-remodeling factor, Fun30(Fft3), as being critical for relieving the nucleosome barrier during RNAPII-mediated elongation, and proposed a model illustrating how Fun30(Fft3) may contribute to nucleosome disassembly during RNAPII-mediated elongation...
September 19, 2017: Transcription
https://www.readbyqxmd.com/read/28925486/long-non-coding-rna-foxd2-as1-functions-as-a-tumor-promoter-in-colorectal-cancer-by-regulating-emt-and-notch-signaling-pathway
#7
X Yang, B Duan, X Zhou
OBJECTIVE: A growing number of long noncoding RNAs (lncRNAs) are emerging as new modulators in cancer origination and progression. However, the functions and molecular mechanisms of lncRNAs to colorectal cancer (CRC) are still largely unknown. The aim of this study was to investigate the function and role of lncRNA FOXD2-AS1 (FOXD2-AS1) in human CRC. PATIENTS AND METHODS: The expression of FOXD2-AS1 was investigated using Real-time reverse transcription-polymerase chain reaction (qRT-PCR) in 45 CRC specimens and matched adjacent normal tissues and CRC cell lines...
August 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/28923911/how-ribosomes-translate-cancer
#8
REVIEW
Sergey O Sulima, Isabel J F Hofman, Kim De Keersmaecker, Jonathan D Dinman
A wealth of novel findings, including congenital ribosomal mutations in ribosomopathies and somatic ribosomal mutations in various cancers, have significantly increased our understanding of the relevance of ribosomes in oncogenesis. Here, we explore the growing list of mechanisms by which the ribosome is involved in carcinogenesis-from the hijacking of ribosomes by oncogenic factors and dysregulated translational control, to the effects of mutations in ribosomal components on cellular metabolism. Of clinical importance, the recent success of RNA polymerase inhibitors highlights the dependence on "onco-ribosomes" as an Achilles' heel of cancer cells and a promising target for further therapeutic intervention...
September 18, 2017: Cancer Discovery
https://www.readbyqxmd.com/read/28923830/the-role-of-crp-and-atg9b-expression-in-clear-cell-renal-cell-carcinoma
#9
Zheng Ma, Zengguang Qi, Jiangsong Li, Jing Yang, Zhonghua Xu
Background: The purpose of the study is to investigate the correlation between the expression of C-reactive protein ( CRP ) and autophagy-related 9B ( ATG9B ) and pathological features of clear cell renal cell carcinoma (CCRCC) patients. We also intended to explore the effects of manipulated expression of CRP and ATG9B on the apoptosis and cell cycle progressing of CCRCC cell line. Material and Methods : ATG9B expression in CCRCC tissues and adjacent renal tissues was analyzed by immunohistochemistry (IHC)...
September 18, 2017: Bioscience Reports
https://www.readbyqxmd.com/read/28923653/effects-of-the-mammalian-target-of-rapamycin-inhibitor-everolimus-on-hepatitis-c-virus-replication-in%C3%A2-vitro-and-in%C3%A2-vivo
#10
A Frey, E-M Ecker, K Piras-Straub, A Walker, T G Hofmann, J Timm, B B Singer, G Gerken, K Herzer
BACKGROUND: The influence of immunosuppressants on hepatitis C virus (HCV) re-infection after liver transplantation, particularly mammalian target of rapamycin inhibitors, remains unclear. The aim of our study was to analyze the influence of everolimus (EVR) on HCV replication activity in the context of underlying molecular mechanisms, with focus on the pro-myelocytic leukemia protein (PML). METHODS: HCV viral load was recorded in 40 patients with post-transplant HCV re-infection before and 8 weeks after introduction of EVR...
October 2017: Transplantation Proceedings
https://www.readbyqxmd.com/read/28923603/unraveling-the-expression-of-genes-involved-in-the-biosynthesis-pathway-of-cardiolipin-and-phosphatidylethalonamine-in-salmonella-hadar-grown-under-static-magnetic-field-200%C3%A2-mt
#11
Ramla Ben Mouhoub, Ahlem Mansouri, Khadidja Aliliche, Hamida Beghalem, Ahmed Landoulsi, Alya El May
We aim in this work to evaluate the effect of static magnetic field 200 mT (SMF) on the expression of genes involved in the biosynthetic pathway of cardiolipin: g3pd, plsB, cdsA, pgsA, pgpA, cls and phosphatidylethanolamine: pssA and psd in Salmonella enterica subsp Enterica serovar Hadar. Bacteria were exposed to a SMF during 3, 6 and 9 h. RNA extraction was followed by Reverse Transcriptase Polymerase Chain Reaction RT-PCR. The relative quantification of mRNA expression levels using 16S rRNA doesn't change during the time exposure...
September 15, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28923079/endoplasmic-reticulum-stress-cooperates-with-toll-like-receptor-ligation-in-driving-activation-of-rheumatoid-arthritis-fibroblast-like-synoviocytes
#12
Pawel A Kabala, Chiara Angiolilli, Nataliya Yeremenko, Aleksander M Grabiec, Barbara Giovannone, Desiree Pots, Timothy R Radstake, Dominique Baeten, Kris A Reedquist
BACKGROUND: Endoplasmic reticulum (ER) stress has proinflammatory properties, and transgenic animal studies of rheumatoid arthritis (RA) indicate its relevance in the process of joint destruction. Because currently available studies are focused primarily on myeloid cells, we assessed how ER stress might affect the inflammatory responses of stromal cells in RA. METHODS: ER stress was induced in RA fibroblast-like synoviocytes (FLS), dermal fibroblasts, and macrophages with thapsigargin or tunicamycin alone or in combination with Toll-like receptor (TLR) ligands, and gene expression and messenger RNA (mRNA) stability was measured by quantitative polymerase chain reaction...
September 18, 2017: Arthritis Research & Therapy
https://www.readbyqxmd.com/read/28923054/a-novel-method-for-extracting-nucleic-acids-from-dried-blood-spots-for-ultrasensitive-detection-of-low-density%C3%A2-plasmodium-falciparum-and-plasmodium-vivax%C3%A2-infections
#13
Kayvan Zainabadi, Matthew Adams, Zay Yar Han, Hnin Wai Lwin, Kay Thwe Han, Amed Ouattara, Si Thura, Christopher V Plowe, Myaing M Nyunt
BACKGROUND: Greater Mekong Subregion countries are committed to eliminating Plasmodium falciparum malaria by 2025. Current elimination interventions target infections at parasite densities that can be detected by standard microscopy or rapid diagnostic tests (RDTs). More sensitive detection methods have been developed to detect lower density "asymptomatic" infections that may represent an important transmission reservoir. These ultrasensitive polymerase chain reaction (usPCR) tests have been used to identify target populations for mass drug administration (MDA)...
September 18, 2017: Malaria Journal
https://www.readbyqxmd.com/read/28922779/targeting-the-myofibroblastic-cancer-associated-fibroblast-phenotype-through-inhibition-of-nox4
#14
Christopher J Hanley, Massimiliano Mellone, Kirsty Ford, Steve M Thirdborough, Toby Mellows, Steven J Frampton, David M Smith, Elena Harden, Cedric Szyndralewiez, Marc Bullock, Fergus Noble, Karwan A Moutasim, Emma V King, Pandurangan Vijayanand, Alex H Mirnezami, Timothy J Underwood, Christian H Ottensmeier, Gareth J Thomas
Background: Cancer-associated fibroblasts (CAFs) are tumor-promoting and correlate with poor survival in many cancers, which has led to their emergence as potential therapeutic targets. However, effective methods to manipulate these cells clinically have yet to be developed. Methods: CAF accumulation and prognostic significance in head and neck cancer (oral, n = 260; oropharyngeal, n = 271), and colorectal cancer (n = 56) was analyzed using immunohistochemistry...
January 1, 2018: Journal of the National Cancer Institute
https://www.readbyqxmd.com/read/28922636/effects-of-mutations-on-active-site-conformation-and-dynamics-of-rna-dependent-rna-polymerase-from-coxsackievirus-b3
#15
Hujun Shen, Mingsen Deng, Yachao Zhang
Recent crystal structures of RNA-dependent RNA polymerase (3D(pol)) from Coxsackievirus B3 (CVB3) revealed that a tyrosine mutation at Phe364 (F364Y) resulted in structures with open active site whereas a hydrophobic mutation at Phe364 (F364A) led to conformations with closed active site. Besides, the crystal structures showed that the F364W mutation had no preference between the open and closed active sites, similar to wild-type. In this paper, we present a molecular dynamics (MD) study on CVB3 3D(pol) in order to address some important questions raised by experiments...
September 12, 2017: Journal of Molecular Graphics & Modelling
https://www.readbyqxmd.com/read/28922369/crimean-congo-hemorrhagic-fever-virus-nucleocapsid-protein-has-dual-rna-binding-modes
#16
Subbiah Jeeva, Sean Pador, Brittany Voss, Safder Saieed Ganaie, Mohammad Ayoub Mir
Crimean Congo hemorrhagic fever, a zoonotic viral disease, has high mortality rate in humans. There is currently no vaccine for Crimean Congo hemorrhagic fever virus (CCHFV) and chemical interventions are limited. The three negative sense genomic RNA segments of CCHFV are specifically encapsidated by the nucleocapsid protein into three ribonucleocapsids, which serve as templates for the viral RNA dependent RNA polymerase. Here we demonstrate that CCHFV nucleocapsid protein has two distinct binding modes for double and single strand RNA...
2017: PloS One
https://www.readbyqxmd.com/read/28921535/genome-wide-host-rna-signatures-of-infectious-diseases-discovery-and-clinical-translation
#17
REVIEW
Harriet D Gliddon, Jethro A Herberg, Michael Levin, Myrsini Kaforou
The use of whole blood gene expression to derive diagnostic biomarkers capable of distinguishing between phenotypically similar diseases holds great promise but remains a challenge. Differential gene expression analysis is used to identify the key genes that undergo changes in expression relative to healthy individuals, as well as to patients with other diseases. These key genes can act as diagnostic, prognostic and predictive markers of disease. Gene expression 'signatures' in the blood hold potential to be used for the diagnosis of infectious diseases, where current diagnostics are unreliable, ineffective or of limited potential...
September 16, 2017: Immunology
https://www.readbyqxmd.com/read/28920849/flavimarina-flava-sp-nov-isolated-from-salicornia-herbacea
#18
Eui-Sang Cho, In-Tae Cha, Jung-Min Park, Hak-Jong Choi, Jong Hun Lee, Seong Woon Roh, Eun-Ah Cho, Mee-Hyang Kweon, Young-Do Nam, Myung-Ji Seo
A Gram-stain-negative, motile-by-gliding, aerobic, non-spore-forming, rod-shaped and yellow-pigmented bacterium was isolated from Salicornia herbacea in the Yellow Sea and designated as strain MBLN091T. It belonged to the family Flavobacteriaceae. The 16S rRNA gene sequence of this isolated strain was similar to that of Flavimarina pacifica IDSW-73T with 94.8 % similarity, and with 92.3-92.8 % similarities to those of other closely related species of the genus Leeuwenhoekiella. The similarities of the RNA polymerase subunit B gene between this strain and F...
September 18, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28920565/regulatory-functions-of-trehalose-6-phosphate-synthase-in-the-chitin-biosynthesis-pathway-in-tribolium-castaneum-coleoptera-tenebrionidae-revealed-by-rna-interference
#19
Q W Chen, S Jin, L Zhang, Q D Shen, P Wei, Z M Wei, S G Wang, B Tang
RNA interference (RNAi) is a very effective technique for studying gene function and may be an efficient method for controlling pests. Trehalose-6-phosphate synthase (TPS), which plays a key role in the synthesis of trehalose and insect development, was cloned in Tribolium castaneum (Herbst) (TcTPS) and the putative functions were studied using RNAi via the injection of double-stranded RNA (dsRNA) corresponding to conserved TPS and trehalose-6-phosphate phosphatase domains. Expression analyses show that TcTPS is expressed higher in the fat body, while quantitative real-time polymerase chain reaction results show that the expression of four trehalase isoforms was significantly suppressed by dsTPS injection...
September 18, 2017: Bulletin of Entomological Research
https://www.readbyqxmd.com/read/28919801/transforming-growth-factor-%C3%AE-1-smad3-independent-epithelial-mesenchymal-transition-in-type-i-collagen-glomerulopathy
#20
Amanda C Brodeur, Anna M Roberts-Pilgrim, Kimberlee L Thompson, Craig L Franklin, Charlotte L Phillips
The glomerulofibrotic Col1a2-deficient mouse model demonstrates glomerular homotrimeric type I collagen deposition in mesangial and subendothelial spaces. In this report, we investigate the role of transforming growth factor β1 (TGF-β1) in myofibroblast activation and epithelial-mesenchymal transition (EMT) in this glomerulopathy. Immunohistochemical analyses of glomerular α-sma, desmin, vimentin, and proliferating cell nuclear antigen demonstrated parietal epithelial cell proliferation and EMT in late stages of the glomerulopathy in the Col1a2-deficient mice...
2017: International Journal of Nephrology and Renovascular Disease
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