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https://www.readbyqxmd.com/read/28807295/anthelmintic-effects-of-forage-chicory-cichorium-intybus-against-free-living-and-parasitic-stages-of-cooperia-oncophora
#1
Miguel Peña-Espinoza, Andrew R Williams, Stig M Thamsborg, Henrik T Simonsen, Heidi L Enemark
Chicory shows great promise as an anthelmintic forage for grazing ruminants that can reduce reliance on anti-parasitic drugs. Recently, we reported potent anthelmintic effects of chicory-based diets in infected cattle with significant reductions in worm burdens of the abomasal nematode Ostertagia ostertagi, whilst no apparent activity was observed against the small intestinal parasite Cooperia oncophora. To explore this discrepancy, we investigated direct anthelmintic effects of forage chicory against C. oncophora in vitro...
August 30, 2017: Veterinary Parasitology
https://www.readbyqxmd.com/read/28783553/effect-of-advanced-maternal-age-on-maternal-and-neonatal-outcomes-in-assisted-reproductive-technology-pregnancies
#2
Amirhossein Moaddab, Frank A Chervenak, Laurence B Mccullough, Haleh Sangi-Haghpeykar, Amir A Shamshirsaz, Amy Schutt, Sara E Arian, Karin A Fox, Gary A Dildy, Alireza A Shamshirsaz
OBJECTIVES: To compare maternal and neonatal outcomes between women with assisted reproductive technologies pregnancy aged <40, 40-44, 45-49, and ≥50 years. STUDY: Design In a population-level analysis study, all live births by ART identified on birth certificate between 2011 and 2014 were extracted (n=101,494) using data from the Center for Disease Control and Prevention-National Center for Health Statistics (CDC-NCHS). We investigated and compared maternal and neonatal outcomes based on conditions routinely listed on birth certificates...
July 29, 2017: European Journal of Obstetrics, Gynecology, and Reproductive Biology
https://www.readbyqxmd.com/read/28758779/a-longitudinal-study-of-families-formed-through-reproductive-donation-parent-adolescent-relationships-and-adolescent-adjustment-at-age-14
#3
Susan Golombok, Elena Ilioi, Lucy Blake, Gabriela Roman, Vasanti Jadva
The aim of the 6th phase of this longitudinal study was to establish whether children born through assisted reproduction involving reproductive donation were at risk for psychological problems following the transition to adolescence at age 14 and, if so, to examine the nature of these problems and the mechanisms involved. Eighty-seven families formed through reproductive donation, including 32 donor insemination families, 27 egg donation families, and 28 surrogacy families, were compared with 54 natural conception families...
July 31, 2017: Developmental Psychology
https://www.readbyqxmd.com/read/28757393/a-comparative-study-on-the-anti-schistosomal-and-hepatoprotective-effects-of-vinpocetine-and-isosorbide-5-mononitrate-on-schistosoma-mansoni-infected-mice
#4
Samar M Alhusseiny, Samar N El-Beshbishi, Maha M Abu Hashim, Hosam El-Dein E El-Nemr, Aya E Handoussa
Schistosomiasis is a remarkable public health problem in developing countries. Presently, praziquantel is the optional drug for all human schistosomiasis. Owing to the increased praziquantel resistance, there is an urgent need to develop new alternatives. This study aims at determining the anti-schistosomal and/or the hepatoprotective effects of the anti-inflammatory drug; vinpocetine, and the vasodilator and the nitric oxide donor; isosorbide-5-mononitrate, in comparison to praziquantel. In the present research, the therapeutic efficacies of these drugs were assessed in Swiss albino female mice (CD-I strain) experimentally infected with an Egyptian strain of Schistosoma mansoni, using some general, parasitological, and histopathological parameters...
July 27, 2017: Acta Tropica
https://www.readbyqxmd.com/read/28751723/hydrogen-sulphide-donors-selectively-potentiate-a-green-tea-polyphenol-egcg-induced-apoptosis-of-multiple-myeloma-cells
#5
Jaehoon Bae, Motofumi Kumazoe, Shuya Yamashita, Hirofumi Tachibana
Hydrogen sulphide (H2S) is a colourless gas with the odour of rotten eggs and has recently been recognized as a signal mediator in physiological activities related with the regulation of homeostasis, the vascular system and the inflammatory system. Here we show that H2S donors, including sodium hydrogen sulphide (NaHS), GYY 4137 and diallyltrisulfide (DATS), synergistically enhanced the anti-cancer effect of a green tea polyphenol (-)-epigallocatechin-3-O-gallate (EGCG) against multiple myeloma cells without affecting normal cells...
July 27, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28747071/a-hibernation-like-state-for-transplantable-organs-is-h-sub-2-sub-s-therapy-the-future-of-organ-preservation
#6
George Dugbartey, Hjalmar Bouma, Manujendra N Saha, Ian Lobb, Robert H Henning, Alp Sener
Renal transplantation is the treatment of choice for end-stage renal disease, during which renal grafts from deceased donors are routinely cold stored to suppress metabolic demand and thereby limit ischemic injury. However, prolonged cold storage followed by reperfusion induces extensive tissue damage termed cold ischemia/reperfusion injury (IRI) and puts the graft at risk of both early and late rejection. The underlying mechanism of IRI is not completely understood and a reliable/ suitable method to protect the renal graft against cold IRI is lacking...
July 26, 2017: Antioxidants & Redox Signaling
https://www.readbyqxmd.com/read/28740754/the-time-course-of-broiler-intestinal-microbiota-development-after-administration-of-cecal-contents-to-incubating-eggs
#7
Erin E Donaldson, Dragana Stanley, Robert J Hughes, Robert J Moore
BACKGROUND: The microbial populations that inhabit the gastrointestinal tract (GIT) are known to influence the health and growth performance of the host. Clean hatcheries and machine-based incubation practices in the commercial poultry industry can lead to the acquisition of aberrant microbiota in the GIT of chickens and a very high level of bird-to-bird variation. The lack of microbial profile flock uniformity presents challenges for harnessing and manipulating intestinal bacteria to better serve the host...
2017: PeerJ
https://www.readbyqxmd.com/read/28721183/infertility-in-the-global-south-raising-awareness-and-generating-insights-for-policy-and-practice
#8
T Gerrits, F Van Rooij, T Esho, W Ndegwa, J Goossens, A Bilajbegovic, A Jansen, B Kioko, L Koppen, S Kemunto Migiro, S Mwenda, H Bos
Infertility is a highly prevalent reproductive health condition in the global South, which often has a devastating impact on the people concerned. Yet, thus far it hardly received any attention from policy makers, Non-Governmental Organizations (NGOs) or donors working in the field of Sexual and Reproductive Health and Rights (SRHR). For this reason we have set up a project to increase knowledge and awareness about infertility and childlessness among those stakeholders and organizations and to generate insight into (possible) interventions in this field...
March 2017: Facts, Views & Vision in ObGyn
https://www.readbyqxmd.com/read/28708521/flow-cytometric-enrichment-of-pacific-bluefin-tuna-type-a-spermatogonia-based-on-light-scattering-properties
#9
REVIEW
Kensuke Ichida, Kazuyoshi Kise, Tetsuro Morita, Ryosuke Yazawa, Yutaka Takeuchi, Goro Yoshizaki
We previously established surrogate broodstock in which the donor germ cells transplanted into the peritoneal cavities of xenogeneic recipients were capable of developing into functional eggs and sperm in teleost fish. In this transplantation system, only the undifferentiated germ cells such as type A spermatogonia (ASG) or a portion of the ASG population were capable of being incorporated into the genital ridges of the recipients and undergo gametogenesis. Therefore, the use of enriched ASGs can be expected to achieve efficient donor-cell incorporation...
June 22, 2017: Theriogenology
https://www.readbyqxmd.com/read/28697908/sperm-donor-conception-and-disclosure-to-children-a-10-year-retrospective-follow-up-study-of-parental-attitudes-in-one-french-center-for-the-study-and-preservation-of-eggs-and-sperm-cecos
#10
Thierry Lassalzede, Marine Paci, Jocelyne Rouzier, Sophie Carez, Audrey Gnisci, Jacqueline Saias-Magnan, Carole Deveze, Jeanne Perrin, Catherine Metzler-Guillemain
OBJECTIVE: To evaluate the percentage of parents in one French center for the study and preservation of eggs and sperm who disclose their use of donated spermatozoa to their children. DESIGN: A questionnaire survey of couples who had a child using donated spermatozoa. SETTING: University hospital laboratory. PATIENT(S): One hundred five couples. INTERVENTION(S): Questionnaire sent by mail. MAIN OUTCOME MEASURE(S): The percentage of parents who disclose their use of donated spermatozoa to their child...
July 8, 2017: Fertility and Sterility
https://www.readbyqxmd.com/read/28697393/same-medicine-different-reasons-comparing-women-s-bodily-experiences-of-producing-eggs-for-pregnancy-or-for-profit
#11
Rene Almeling, Iris L Willey
Women doing in vitro fertilization (IVF) to have a child describe it as painful and emotionally draining. Egg donors undergo the same medical regimen for a different reason - to produce eggs for another woman in exchange for thousands of dollars - and describe it as quick and relatively painless. Medical researchers typically compare bodily responses by variables such as gender, age, and health status. We use the case of "egg production" to propose a new factor that may be an important source of variation in bodily experience: an individual's reason for undergoing the medical intervention in the first place...
June 22, 2017: Social Science & Medicine
https://www.readbyqxmd.com/read/28686488/contribution-of-both-b-cell-intrinsic-alterations-as-well-as-non-hematopoietic-derived-factors-in-the-enhanced-immune-response-of-the-nod-mouse
#12
Viqar Showkat Banday, Radha Thyagarajan, Kristina Lejon
The underlying cellular and molecular mechanism for the development of Type 1 diabetes is still to be fully revealed. We have previously demonstrated that the NOD mouse, a model for Type 1 diabetes, display a prolonged and enhanced immune response to both self and non-self-antigens. The molecular explanation for this defect however, has not been determined. In this study we immunized NOD and C57BL/6 (B6) with the conventional antigen i.e. hen egg lysozyme (HEL) and analyzed B cell activation, germinal center reaction and antibody clearance...
July 7, 2017: Autoimmunity
https://www.readbyqxmd.com/read/28683668/kinship-identities-in-the-context-of-uk-maternal-spindle-transfer-and-pronuclear-transfer-legislation
#13
Calum MacKellar
In the discussions leading up to the enactment of the UK Human Fertilisation and Embryology (Mitochondrial Donation) Regulations 2015, it was repeatedly emphasised, by many commentators, that maternal spindle transfer (MST) and pronuclear transfer (PNT) did not give rise to children who could be considered as having three or more parents. This was because it was argued that only the genetic material found in the chromosomes should be considered as the determining factor for the formation of parent-child relationships and the resulting kinship identities...
July 6, 2017: New Bioethics: a Multidisciplinary Journal of Biotechnology and the Body
https://www.readbyqxmd.com/read/28663368/betaine-is-accumulated-via-transient-choline-dehydrogenase-activation-during-mouse-oocyte-meiotic-maturation
#14
Taylor McClatchie, Megan Meredith, Mariame O Ouédraogo, Sandy Slow, Michael Lever, Mellissa R W Mann, Steven H Zeisel, Jacquetta M Trasler, Jay M Baltz
Betaine (N,N,N-trimethylglycine) plays key roles in mouse eggs and preimplantation embryos first in a novel mechanism of cell volume regulation and second as a major methyl donor in blastocysts, but its origin is unknown. Here, we determined that endogenous betaine was present at low levels in germinal vesicle (GV) stage mouse oocytes before ovulation, but reached high levels in the mature, ovulated egg. However, no betaine transport into oocytes was detected during meiotic maturation. Since betaine can be synthesized in mammalian cells via choline dehydrogenase (CHDH; EC1...
June 29, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28635412/gamete-donors-reasons-for-and-expectations-and-experiences-of-registration-with-a-voluntary-donor-linking-register
#15
Eric Blyth, Marilyn Crawshaw, Lucy Frith, Olga van den Akker
This paper reports on a study of the views and experiences of 21 sperm donors and five egg donors registered with UK DonorLink (UKDL), a voluntary DNA-based contact register established to facilitate contact between adults who wish to identify and locate others to whom they are genetically related following donor conception. Specifically, the paper examines donors' reasons for searching for, or making information about themselves available to donor-conceived offspring. Their expectations of registration with UKDL, experiences of being registered and finally, the experiences of those who had contacted donor-conceived offspring and other genetic relatives are investigated...
February 21, 2017: Human Fertility: Journal of the British Fertility Society
https://www.readbyqxmd.com/read/28625761/impact-of-vitamin-d-levels-on-ovarian-reserve-and-ovarian-response-to-ovarian-stimulation-in-oocyte-donors
#16
Alberta Maria Fabris, Maria Cruz, Carlos Iglesias, Alberto Pacheco, Azadeh Patel, Jayesh Patel, Human Fatemi, Juan Antonio García-Velasco
Recently, growing interest in vitamin D has emerged from findings that demonstrate a low vitamin D status in populations. Similarly, much interest has been shown in the role that anti-Müllerian hormone (AMH) plays in reproductive physiology. Considerable confusion as to whether vitamin D status is related to ovarian function can be found in the literature. Our retrospective study was performed from June 2014 to April 2015. Oocyte donors were recruited and stimulated under the antagonist protocol with gonadotrophin-releasing hormone (GnRH) agonist to trigger ovulation...
May 19, 2017: Reproductive Biomedicine Online
https://www.readbyqxmd.com/read/28589355/germinal-center-formation-with-retrovirally-transduced-b-cells-for-determining-the-role-of-specific-molecules-in-vivo
#17
Rinako Nakagawa
Retrovirus-mediated gene transfer has become a powerful tool to investigate roles of specific molecules in B cells, due to its efficiency and expeditiousness. This technology is applicable to activated B cells in order to determine effects of a gene of interest during germinal center (GC) reactions in combination with adoptive transfer. To achieve this, B cells derived from SWHEL mice expressing hen egg lysozyme (HEL)-specific B cell receptors (BCR) are stimulated with HEL antigen in vivo and then with anti-CD40 antibody ex vivo...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28554074/photochemistry-and-mechanism-of-designed-pyrenyl-probe-towards-promoted-cleavage-of-proteins
#18
Sudarat Yenjai, Mayuso Kuno, Siritron Samosorn, Teerayuth Liwporncharoenvong, Apinya Buranaprapuk
A new photochemical reagent, succinic acid-1(1-pyrene)methylamide (PMA-SUC), was developed to recognize the specific binding sites on model proteins, egg-white lysozyme and avidin. The interaction of the photochemical reagent with the proteins was studied by UV-Vis, fluorescence spectroscopic methods and docking description. PMA-SUC was found to bind to lysozyme and avidin with binding constants (Kb) of 2.4×10(5) and 6.7×10(5) (M(-1)), respectively. The fluorescence intensity of PMA-SUC decreased with increasing concentration of both proteins...
May 22, 2017: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/28549399/a-systematic-review-investigating-psychosocial-aspects-of-egg-sharing-in-the-united-kingdom-and-their-potential-effects-on-egg-donation-numbers
#19
Timothy Bracewell-Milnes, Srdjan Saso, Hossam Abdalla, Meen-Yau Thum
This review aims to provide an up-to-date knowledge of the psychosocial aspects of egg donation from the perspectives of the egg share donor and their recipient. It explores the motives, experiences and attitudes of egg sharers and their views towards donor anonymity and disclosure. Conclusions are made on how these findings can guide clinical practice and improve egg sharing numbers. A systematic search of peer-reviewed journals of four computerized databases was undertaken. Eleven studies were included in the review...
May 26, 2017: Human Fertility: Journal of the British Fertility Society
https://www.readbyqxmd.com/read/28545561/sources-of-variability-in-the-measurement-of-ascaris-lumbricoides-infection-intensity-by-kato-katz-and-qpcr
#20
Alice V Easton, Rita G Oliveira, Martin Walker, Elise M O'Connell, Sammy M Njenga, Charles S Mwandawiro, Joanne P Webster, Thomas B Nutman, Roy M Anderson
BACKGROUND: Understanding and quantifying the sources and implications of error in the measurement of helminth egg intensity using Kato-Katz (KK) and the newly emerging "gold standard" quantitative polymerase chain reaction (qPCR) technique is necessary for the appropriate design of epidemiological studies, including impact assessments for deworming programs. METHODS: Repeated measurements of Ascaris lumbricoides infection intensity were made from samples collected in western Kenya using the qPCR and KK techniques...
May 25, 2017: Parasites & Vectors
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