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https://www.readbyqxmd.com/read/29715049/pregnancy-achieved-using-donor-eggs-in-cancer-survivors-with-treatment-induced-ovarian-failure-obstetric-and-perinatal-outcome
#1
Anna Marklund, Josefine Nasiell, Ann-Sofie Berger, Antonia Fagerberg, Kenny A Rodriguez-Wallberg
BACKGROUND: Egg donation is a common fertility treatment in female cancer survivors with reproductive wish and iatrogenic ovarian failure. We examined whether women previously treated for cancer have a higher risk of pregnancy complications after egg donation treatments when compared to women without cancer history. METHODS: In this prospective cohort treated during 2003-2015 at a single center, 31 women with previous history of cancer achieved 25 deliveries and 212 women without cancer history achieved 244 deliveries...
May 1, 2018: Journal of Women's Health
https://www.readbyqxmd.com/read/29713890/pain-perception-following-epithelialized-gingival-graft-harvesting-a-randomized-clinical-trial
#2
Lorenzo Tavelli, Andrea Ravidà, Muhammad H A Saleh, Bartosz Maska, Fernando Suárez-López Del Amo, Giulio Rasperini, Hom-Lay Wang
OBJECTIVES: The aim of this study was to compare the effects of a hemostatic collagen sponge and a collagen sponge sealed with a bio-adhesive material on the palatal donor sites with the purpose of minimizing postoperative pain after epithelialized gingival graft (EGG) harvesting. MATERIAL AND METHODS: The present study consisted of 44 EGGs harvested in 44 patients. In the control group, a hemostatic collagen sponge was applied over the palatal wound, while the test group was treated with additional cyanoacrylate...
April 30, 2018: Clinical Oral Investigations
https://www.readbyqxmd.com/read/29709727/clinical-outcomes-of-a-vitrified-donor-oocyte-programme-a-single-uk-centre-experience
#3
S Seshadri, W Saab, H Exeter, E Drew, A Petrie, M Davies, P Serhal
OBJECTIVE: To assess the survival rate of vitrified oocytes used in an egg recipient programme and compare the clinical outcomes of pregnancy and live-birth rates per warmed oocyte with fresh autologous oocytes. The differences in the obstetrical outcomes between the two groups were also studied. DESIGN: A prospective case control study from a single in-vitro fertilisaton (IVF) Centre in UK SETTING: Centre of Reproductive and Genetic Health (CRGH), London POPULATION: Vitrified oocytes from egg donors and autologous fresh oocytes from patients attending for an IVF cycle METHODS: The study group consisted of 1490 vitrified oocytes, which were obtained from 145 egg donors who underwent a stimulation cycle at CRGH Centre...
April 22, 2018: European Journal of Obstetrics, Gynecology, and Reproductive Biology
https://www.readbyqxmd.com/read/29701833/the-perspectives-of-adolescents-conceived-using-surrogacy-egg-or-sperm-donation
#4
S Zadeh, E C Ilioi, V Jadva, S Golombok
STUDY QUESTION: What are the perspectives of adolescents conceived using surrogacy, egg or sperm donation regarding their conception and the third party involved? SUMMARY ANSWER: The majority of adolescents described feeling indifferent about their conception, and yet simultaneously reported an interest in the third party involved, or were in contact with them. WHAT IS KNOWN ALREADY: There is an assumption that children conceived through reproductive donation will feel negatively about their origins in adolescence...
April 12, 2018: Human Reproduction
https://www.readbyqxmd.com/read/29662093/application-of-interspecific-somatic-cell-nuclear-transfer-iscnt-in-sturgeons-and-an-unexpectedly-produced-gynogenetic-sterlet-with-homozygous-quadruple-haploid
#5
Effrosyni Fatira, Miloš Havelka, Catherine Labbé, Alexandra Depincé, Viktoriia Iegorova, Martin Pšenička, Taiju Saito
Somatic cell nuclear transfer (SCNT) is a very promising cloning technique for reconstruction of endangered animals. The aim of the present research is to implement the interspecific SCNT (iSCNT) technique to sturgeon; one fish family bearing some of the most critically endangered species. We transplanted single cells enzymatically isolated from a dissociated fin-fragment of the Russian sturgeon (Acipenser gueldenstaedtii) into non-enucleated eggs of the sterlet (Acipenser ruthenus), two species bearing different ploidy (4n and 2n, respectively)...
April 16, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29649725/production-of-live-fish-derived-from-frozen-germ-cells-via-germ-cell-transplantation
#6
Goro Yoshizaki, Seungki Lee
The conservation of endangered fish is an urgent issue. Although cryo-banking of fish gametes might ultimately help conserve endangered fish, cryopreservation of fish eggs or embryos is still not possible due to their large size and high yolk content. Therefore, as an alternative, we focused on undifferentiated germ cells, such as primordial germ cells, spermatogonia, and oogonia, as materials for cryopreservation. Transplantation of cryopreserved germ cells into the body cavity of allogeneic or xenogeneic recipients sterilized by triploidization or endogenous germ cell ablation resulted in migration of the transplanted germ cells toward the recipient genital ridges, where they were eventually incorporated...
April 3, 2018: Stem Cell Research
https://www.readbyqxmd.com/read/29649432/nitric-oxide-signaling-regulates-tumor-induced-intussusceptive-like-angiogenesis
#7
Selvaraj Vimalraj, Srinivasan Bhuvaneswari, Sundaresan Lakshmikirupa, Ganesh Jyothsna, Suvro Chatterjee
Existing animal models for screening tumor angiogenic process have various setbacks that necessitate further investigations. In this study, we developed an ex-ovo egg yolk angiogenesis model to screen the angiogenic potency of tumor cells (HeLa and SiHa cell lines). The egg yolk angiogenesis assay was applied to study the nitric oxide (NO) influence on switching from sprouting angiogenesis (SA) to intussusceptive angiogenesis (IA) under tumor microenvironment. Morphological analysis and SA-like or IA-like markers expression were determined during the development of chicken chorioallantoic membrane (CAM) from day 5 to 13...
April 9, 2018: Microvascular Research
https://www.readbyqxmd.com/read/29573495/efficient-in-planta-gene-targeting-in-arabidopsis-using-egg-cell-specific-expression-of-the-cas9-nuclease-of-s-aureus
#8
Felix Wolter, Jeanette Klemm, Holger Puchta
Gene targeting (GT), the programmed change of genomic sequences by homologous recombination (HR), is still a major challenge in plants. We previously developed an in planta GT strategy by simultaneously releasing from the genome a dsDNA donor molecule and creating a DSB at a specific site within the targeted gene. Using Cas9 form S. pyogenes (SpCas9) under the control of a ubiquitin gene promoter, we obtained seeds harbouring GT events, although at a low frequency. In the present research we tested different developmentally controlled promotors and different kinds of DNA lesions for their ability to enhance GT of the acetolactate synthase (ALS) gene of Arabidopsis...
March 24, 2018: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/29573423/toxic-money-or-paid-altruism-the-meaning-of-payments-for-identity-release-gamete-donors
#9
Leah Gilman
Public discourses commonly frame gamete, organ and other forms of bodily donation as altruistic 'gifts'. However, despite on-going debates about the ethics of payments to donors, few studies have examined the views of donors themselves regarding the meaning of payments and their compatibility (or not) with understandings of these practices as gifts. This article addresses this issue, analysing 24 in-depth interviews with UK identity-release gamete donors. It was crucial to all participants that their donation be viewed as fundamentally other-oriented, motivated by the desire to help others...
March 24, 2018: Sociology of Health & Illness
https://www.readbyqxmd.com/read/29555418/development-of-a-microbioreactor-for-glycoconjugate-synthesis
#10
Katsuji Haneda, Takefumi Oishi, Hiroshi Kimura, Toshiyuki Inazu
A microbioreactor immobilized with a synthase-type mutant enzyme, Endo-M-N175Q (glycosynthase) of endo-β-N-acetylglucosaminidase derived from Mucor hiemalis (Endo-M), was constructed and used for glycoconjugate synthesis. The transglycosylation was performed with a reaction mixture containing an oxazoline derivative of sialo complex-type glycoside (SG), which was prepared from a sialo complex-type glycopeptide SGP derived from hen egg yolk, as a glycosyl donor and N-Fmoc-N-acetylglucosaminyl-l-asparagine [Fmoc-Asn(GlcNAc)-OH] as an acceptor...
March 9, 2018: Bioorganic & Medicinal Chemistry
https://www.readbyqxmd.com/read/29544143/reproducing-whiteness-and-enacting-kin-in-the-nordic-context-of-transnational-egg-donation-matching-donors-with-cross-border-traveller-recipients-in-finland
#11
Riikka Homanen
The multimillion-euro fertility industry increasingly tailors its treatments to infertile people who are willing to travel across national borders for treatments inaccessible at home, especially reproductive tissue donor treatments. Finland is the Nordic destination for access to donor eggs, particularly for Swedes and Norwegians hoping for a donor match that will achieve a child of phenotypically plausible biological descent. Finns are seen as Nordic kin, and the inheritability of "Nordicness" is reinforced at clinics...
April 2018: Social Science & Medicine
https://www.readbyqxmd.com/read/29542433/is-lower-quality-clinical-care-ethically-justifiable-for-patients-residing-in-areas-with-infrastructure-deficits
#12
Marcia C Inhorn, Pasquale Patrizio
Reproductive health services, including infertility care, are important in countries with infrastructure deficits, such as Lebanon, which now hosts more than one million Syrian refugees. Islamic prohibitions on child adoption and third-party reproductive assistance (donor eggs, sperm, embryos, and surrogacy) mean that most Muslim couples must turn to in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) to overcome their childlessness. Attempts to bring low-cost IVF-ICSI to underserved populations might help infertile couples where no other services are available...
March 1, 2018: AMA Journal of Ethics
https://www.readbyqxmd.com/read/29520306/synthetic-and-semi-synthetic-approaches-to-unprotected-n-glycan-oxazolines
#13
REVIEW
Antony J Fairbanks
N -Glycan oxazolines have found widespread use as activated donor substrates for endo-β- N -acetylglucosaminidase (ENGase) enzymes, an important application that has correspondingly stimulated interest in their production, both by total synthesis and by semi-synthesis using oligosaccharides isolated from natural sources. Amongst the many synthetic approaches reported, the majority rely on the fabrication (either by total synthesis, or semi-synthesis from locust bean gum) of a key Manβ(1-4)GlcNAc disaccharide, which can then be elaborated at the 3- and 6-positions of the mannose unit using standard glycosylation chemistry...
2018: Beilstein Journal of Organic Chemistry
https://www.readbyqxmd.com/read/29510633/-medroxyprogesteron-acetate-use-to-block-lh-surge-in-oocyte-donor-stimulation
#14
REVIEW
I Crha, P Ventruba, E Filipinská, M Dziakova, J Žáková, M Ješeta, E Lousová, Z Papíková
OBJECTIVE: To compare the results of ovarian stimulation with LH surge blockade by medroxyprogesterone acetate or GnRH antagonist in oocytes donors. To present current options of exogenous and endogenous progestins instead of GnRH analogues to block LH surge during ovarian stimulation. DESIGN: Retrospective study of oocyte donor cycles and literature review. SETTING: Department of Obstetrics and Gynecology, Faculty of Medicine, Masaryk university and University Hospital Brno...
2018: Ceská Gynekologie
https://www.readbyqxmd.com/read/29492478/successful-pregnancy-and-live-birth-after-intrauterine-administration-of-autologous-platelet-rich-plasma-in-a-woman-with-recurrent-implantation-failure-a-case-report
#15
Marzie Farimani, Jalal Poorolajal, Soghra Rabiee, Maryam Bahmanzadeh
Background: Platelets contain a significant amount of growth factors that have positive effects on local tissue repair and endometrial receptivity. Case: Here we present a 45-yr-old woman with primary infertility and two failed in vitro fertilization (IVF) cycles who was candidate to receive donor eggs. Five consecutive frozen-thawed embryo transfer cycles with good quality embryos were performed within 2 yr. With the diagnosis of recurrent implantation failure (RIF), the patient was treated for improving endometrial receptivity with intrauterine administration of autologous platelet-rich plasma (PRP), 24 hr before embryo transfer...
December 2017: International Journal of Reproductive Biomedicine (Yazd, Iran)
https://www.readbyqxmd.com/read/29491972/increased-population-density-reduces-body-growth-and-female-investment-in-a-simultaneous-hermaphrodite
#16
Elio Cannarsa, Stefania Meconcelli
Sex allocation theory applied to hermaphrodites assumes that there is a trade off between the allocation of resources to male and female functions, within a fixed reproductive resource budget. Charnov's classic resource allocation model predicts a more female-biased sex allocation when competition among different sperm donors is low due to diminishing fitness returns for male investment. By manipulating the social group size, one automatically changes the population density at which individuals live. Increasing population density may affect reproductive allocation, leading to resource competition and/or to increased concentration of harmful metabolites...
April 2017: Current Zoology
https://www.readbyqxmd.com/read/29477768/inhibition-of-amyloid-fibrillation-of-lysozyme-by-bisdemethoxycurcumin-and-diacetylbisdemethoxycurcumin
#17
Fakhrossadat Mohammadi, Marzieh Moeeni, Afshin Mahmudian, Leila Hassani
Amyloid deposition, arising from the fibrillogenesis of proteins in organs and tissues of the body, causes several neurodegenerative disorders. One therapeutic approach is based on the use of polyphenols and their derivatives for suppressing and inhibiting the accumulation of these toxic fibrils in tissues. In the present study, the anti-amyloidogenic activities of bisdemethoxycurcumin (BDMC), a natural polyphenolic compound, and diacetylbisdemethoxycurcumin (DABC), a synthetic derivative of curcumin, on the amyloid fibrillation of hen egg white lysozyme (HEWL) is studied in depth using thioflavin T (ThT) fluorescence, atomic force microscopy (AFM), circular dichroism spectroscopy (CD), molecular docking and Ligplot calculations...
April 2018: Biophysical Chemistry
https://www.readbyqxmd.com/read/29456842/hydrogen-sulfide-nitric-oxide-and-neurodegenerative-disorders
#18
REVIEW
Sandesh Panthi, Sumeet Manandhar, Kripa Gautam
Hydrogen Sulfide (H2 S) and Nitric Oxide (NO) have become recognized as important gaseous signaling molecules with enormous pharmacological effects, therapeutic value, and central physiological roles. NO is one of the most important regulators of the pathophysiological condition in central nervous system (CNS). It is critical in the various functioning of the brain; however, beyond certain concentration/level, it is toxic. H2 S was regarded as toxic gas with the smell like rotten egg. But, it is now regarded as emerging neuroprotectant and neuromodulator...
2018: Translational Neurodegeneration
https://www.readbyqxmd.com/read/29447661/ethical-quandaries-around-expanded-carrier-screening-in-third-party-reproduction
#19
REVIEW
Heidi Mertes, Steven R Lindheim, Guido Pennings
Although current screening methods of gamete donors are capable of reducing the incidence of genetic anomalies in donor offspring below general population levels, targeted screening for a large number of conditions (expanded carrier screening or ECS) could be considered as part of the routine selection procedure for gamete donors. There are, however, important drawbacks to its practical implementation. Excluding all carriers of severe recessive monogenic pediatric disorders would disqualify virtually all donors, and other approaches negatively affect cost (and therefore access), present dilemmas in regard to disclosure of genetic findings, and/or overburden the intended parents...
February 2018: Fertility and Sterility
https://www.readbyqxmd.com/read/29426347/attitudes-of-sperm-egg-and-embryo-donors-and-recipients-towards-genetic-information-and-screening-of-donors
#20
David J Amor, Annabelle Kerr, Nandini Somanathan, Alison McEwen, Marianne Tome, Jan Hodgson, Sharon Lewis
BACKGROUND: Gamete and embryo donors undergo genetic screening procedures in order to maximise the health of donor-conceived offspring. In the era of genomic medicine, expanded genetic screening may be offered to donors for the purpose of avoiding transmission of harmful genetic mutations. The objective of this study was to explore the attitudes of donors and recipients toward the expanded genetic screening of donors. METHODS: Qualitative interview study with thematic analysis, undertaken in a tertiary fertility centre...
February 9, 2018: Reproductive Health
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