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https://www.readbyqxmd.com/read/29303233/high-percentages-of-embryos-with-21-18-or-13-trisomy-are-related-to-advanced-paternal-age-in-donor-egg-cycles
#1
Javier García-Ferreyra, Roly Hilario, Julio Dueñas
OBJECTIVE: Advanced paternal age is related to poor sperm quality; however, little is known on its effect on aneuploidy embryo rates and, more importantly, on chromosomal abnormalities like trisomy 21, 18 and 13. The objective of this study was to evaluate the effect of advanced paternal age on the trisomy rates of the chromosomes 21, 18 or 13 in embryos obtained from donated oocytes. METHODS: A total of 378 embryos, obtained from 52 IVF/ICSI cycles with donated oocytes in conjunction with PGD, were allocated according to paternal age in three groups: Group A: ≤39 years (n=115 embryos), Group B: 40-49 years (n=157 embryos) and Group C: ≥50 year (n=106 embryos)...
January 5, 2018: JBRA Assisted Reproduction
https://www.readbyqxmd.com/read/29247843/dietary-betaine-supplementation-in-hens-modulates-hypothalamic-expression-of-cholesterol-metabolic-genes-in-f1-cockerels-through-modification-of-dna-methylation
#2
Abdulrahman A Idriss, Yun Hu, Zhen Hou, Yan Hu, Qinwei Sun, Nagmeldin A Omer, Halima Abobaker, Yingdong Ni, Ruqian Zhao
Betaine is widely used in animal nutrition to promote growth, development and methyl donor during methionine metabolism through nutritional reprogramming via regulation of gene expression. Prenatal betaine exposure is reported to modulate hypothalamic cholesterol metabolism in chickens, yet it remains unknown whether feeding hens with betaine-supplemented diet may affect hypothalamic cholesterol metabolism in F1 offspring. In this study, hens were fed with basal or betaine-supplemented (0.5%) for 30days, and the eggs were collected for incubation...
December 14, 2017: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/29243796/can-high-levels-of-d-chiro-inositol-in-follicular-fluid-exert-detrimental-effects-on-blastocyst-quality
#3
K Ravanos, G Monastra, T Pavlidou, M Goudakou, N Prapas
OBJECTIVE: It was previously shown that higher concentrations of myo-inositol in human follicular fluid improve oocyte and embryo quality, whereas D-chiro-inositol seems to worsen oocyte quality and ovarian response in polycystic ovary syndrome patients. Our study was the first one aiming to test whether different myo-inositol and D-chiro-inositol concentration in follicular fluids correlate with blastocyst quality in healthy young women. PATIENTS AND METHODS: Eight egg donors and eleven couples undergoing in vitro fertilization, were involved in a prospective observational study...
December 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/29237004/surrogacy-families-headed-by-gay-men-relationships-with-surrogates-and-egg-donors-fathers-decisions-over-disclosure-and-children-s-views-on-their-surrogacy-origins
#4
Nicola Carone, Roberto Baiocco, Demetria Manzi, Chiara Antoniucci, Victoria Caricato, Eugenio Pagliarulo, Vittorio Lingiardi
STUDY QUESTION: How do gay father families experience surrogacy in terms of their relationships with surrogates and egg donors, fathers' disclosure decisions and children's views on their surrogacy origins? SUMMARY ANSWER: More families had a relationship with the surrogate than the egg donor, and almost all had started to disclose to their children, the majority of whom expressed limited interest in their surrogacy conception. WHAT IS KNOWN ALREADY: Gay fathers tend to report greater contact with the surrogate than the egg donor and to disclose only the use of a surrogate (omitting discussion of the egg donor and the respective fathers' genetic relatedness)...
December 11, 2017: Human Reproduction
https://www.readbyqxmd.com/read/29226264/generation-of-viable-progeny-from-dead-brooders-of-endangered-catfish-clarias-magur-hamilton-1822
#5
Sullip Kumar Majhi, Santosh Kumar
The obligatory air-breathing catfish Clarias magur is a prime candidate for aquaculture owing to its unique taste, high growth rate, and hardy nature. However, recently the IUCN has listed the species under the endangered category because the population has critically declined in the wild. The sexually mature C. magur brooders are often collected from their natural habitats for seed production in captivity. In many cases, the brooder dies due to handling injuries or confinement stress. In this study, we demonstrated that viable progeny could be generated from freshly dead sexually mature C...
October 2017: Heliyon
https://www.readbyqxmd.com/read/29190151/the-effect-of-repeated-light-dark-shifts-on-uterine-receptivity-and-early-gestation-in-mice-undergoing-embryo-transfer
#6
Cathy A Goldstein, Louise M O'Brien, Ingrid L Bergin, Thomas L Saunders
Female shift workers are at increased risk for negative reproductive outcomes, and animal evidence suggests that manipulation of the light-dark cycle is detrimental to early gestation in female mice. Specifically, failure of implantation may be responsible for these findings. The objective of this study was to better delineate which reproductive processes are vulnerable to detrimental effects of maternal circadian disturbance. We exposed mice undergoing embryo transfer to repetitive phase advances of the photoperiod...
November 30, 2017: Systems Biology in Reproductive Medicine
https://www.readbyqxmd.com/read/29181564/a-cadaver-study-into-the-number-of-fasciotomies-required-to-decompress-the-anterior-compartment-in-forearm-compartment-syndrome
#7
Lionel Benamran, Alain Charles Masquelet
PURPOSE: There is no typical approach for decompression of forearm compartment syndrome, due to contradictory considerations regarding the characteristics of forearm anterior compartment deep fascia. The main purpose of this study was to determine how many fasciae should be opened to fully decompress the forearm anterior compartment. Further, the compliance of the deep anterior compartment was also investigated, to strengthen our results. METHODS: An experimental study of a laboratory model of acute forearm compartment syndrome was performed...
November 27, 2017: Surgical and Radiologic Anatomy: SRA
https://www.readbyqxmd.com/read/29159942/horizontal-transmission-of-metarhizium-anisopliae-hypocreales-clavicipitacea-and-the-effects-of-infection-on-oviposition-rate-in-laboratory-populations-of-musca-domestica-diptera-muscidae
#8
Dalton K Baker, Steven J Rice, Diana M Leemon, Peter J James
BACKGROUND: Effective control of house fly, Musca domestica (L.), populations currently relies on the use of chemical insecticides in most situations. Entomopathogenic fungi such as Metarhizium anisopliae (Metschn.) Sorokin may provide an alternative to chemicals and their efficacy may be enhanced by autodissemination amongst flies. This study assessed the capacity of M. anisopliae for transmission between adult M. domestica and the effects of infection on the fecundity of females. RESULTS: Metarhizium anisopliae was transmitted between adult M...
November 21, 2017: Pest Management Science
https://www.readbyqxmd.com/read/29145059/binding-of-naringin-and-naringenin-with-hen-egg-white-lysozyme-a-spectroscopic-investigation-and-molecular-docking-study
#9
Sourav Das, Pooja Ghosh, Sudipta Koley, Atanu Singha Roy
The interactions of naringenin (NG) and naringin (NR) with Hen Egg White Lysozyme (HEWL) in aqueous medium have been investigated using UV-vis spectroscopy, steady-state fluorescence, circular dichroism (CD), Fourier Transform infrared spectroscopy (FT-IR) and molecular docking analyses. Both NG and NR can quench the intrinsic fluorescence of HEWL via static quenching mechanism. At 300K, the value of binding constant (Kb) of HEWL-NG complex (5.596±0.063×10(4)M(-1)) was found to be greater than that of HEWL-NR complex (3...
November 8, 2017: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/29143944/one-thousand-seventy-eight-autologous-ivf-cycles-in-women-45%C3%A2-years-and-older-the-largest-single-center-cohort-to-date
#10
Vinay Gunnala, Mohamad Irani, Alexis Melnick, Zev Rosenwaks, Steven Spandorfer
PURPOSE: The purpose of this study was to determine IVF outcomes in women 45 years and older using autologous oocytes. METHODS: This is a retrospective cohort study reviewing all IVF cycles in women ≥ 45 years old from January 1995 to June 2015 that were conducted at one academic medical center. One thousand seventy-eight fresh, autologous IVF cycles met inclusion criteria. PGD/S, natural IVF, and donor egg cycles were excluded. Outcomes were analyzed for the different age groups (age 45, n = 773; age 46, n = 221; age 47, n = 57; age 48, n = 22; age 49, n = 5)...
November 16, 2017: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/29130972/human-dendritic-cells-with-th2-polarizing-capacity-analysis-using-label-free-quantitative-proteomics
#11
Leonie Hussaarts, Maria Mardalena Martini Kaisar, Arzu Tugce Guler, Hans Dalebout, Bart Everts, André M Deelder, Magnus Palmblad, Maria Yazdanbakhsh
BACKGROUND: Dendritic cells (DCs) are the sentinels of the immune system. Upon recognition of a pathogen, they mature and migrate to draining lymph nodes to prime and polarize T cell responses. Although it is known that helminths and helminth-derived molecules condition DCs to polarize T helper (Th) cells towards Th2, the underlying mechanisms remain incompletely understood. OBJECTIVES: The aim of this study was to conduct a proteome analysis of helminth antigen-stimulated DCs in order to gain more insight into the cellular processes associated with their ability to polarize immune responses...
2017: International Archives of Allergy and Immunology
https://www.readbyqxmd.com/read/29117383/assisted-reproductive-technology-in-europe-2013-results-generated-from-european-registers-by-eshre
#12
C Calhaz-Jorge, C De Geyter, M S Kupka, J de Mouzon, K Erb, E Mocanu, T Motrenko, G Scaravelli, C Wyns, V Goossens
STUDY QUESTION: Are there any changes in the treatments involving ART and IUI initiated in Europe during 2013 compared with previous years? SUMMARY ANSWER: An increase in the overall number of ART cycles resulting from a higher number of countries reporting data was evident, the pregnancy rates (PRs) in 2013 remained stable compared with those reported in 2012, the number of transfers with multiple embryos (3+) was lower than ever before yet the multiple delivery rates (DRs) remained unchanged, and IUI activity and success rates were similar to those of last years...
October 1, 2017: Human Reproduction
https://www.readbyqxmd.com/read/29116706/effect-of-sperm-dna-fragmentation-on-embryo-development-clinical-and-biological-aspects
#13
Cristian Alvarez Sedó, Melina Bilinski, Daniela Lorenzi, Heydy Uriondo, Felicitas Noblía, Valeria Longobucco, Estefanía Ventimiglia Lagar, Florencia Nodar
OBJECTIVE: The aim of this study was to investigate the effect of sperm DNA fragmentation on fertilization rate, embryo development (blastulation rate), and pregnancy outcomes for ICSI cycles performed in a cohort of couples using donor eggs and to assess the remaining embryos that were not transferred or frozen for apoptotic markers. METHODS: Eighty-two women (egg recipients) were included in the study (2016) were included in the study. The recipients' mean age was 41...
November 8, 2017: JBRA Assisted Reproduction
https://www.readbyqxmd.com/read/29070706/does-egg-donation-for-mitochondrial-replacement-techniques-generate-parental-responsibilities
#14
César Palacios-González
Children created through mitochondrial replacement techniques (MRTs) are commonly presented as possessing 50% of their mother's nuclear DNA, 50% of their father's nuclear DNA and the mitochondrial DNA of an egg donor. This lab-engineered genetic composition has prompted two questions: Do children who are the product of an MRT procedure have three genetic parents? And, do MRT egg donors have parental responsibilities for the children created? In this paper, I address the second question and in doing so I also address the first one...
October 25, 2017: Journal of Medical Ethics
https://www.readbyqxmd.com/read/29053854/dietary-betaine-supplementation-increases-adrenal-expression-of-steroidogenic-acute-regulatory-protein-and-yolk-deposition-of-corticosterone-in-laying-hens
#15
Halima Abobaker, Yun Hu, Zhen Hou, Qinwei Sun, Abdulrahman A Idriss, Nagmeldin A Omer, Yibo Zong, Ruqian Zhao
Betaine, an important methyl donor, is known to execute epigenetic regulation of gene expression via nutritional reprogramming. Herein, we explore whether feeding a betaine-supplemented diet to laying hens would affect corticosteroid biosynthesis in the adrenal gland and corticosterone deposition in eggs, in correlation with the expression of methyl transfer enzymes and the promoter DNA methylation status of affected genes. Rugao yellow-feathered laying hens at 38 weeks of age were assigned to Control and Betaine groups, fed basal and betaine-supplemented diets, respectively, for four weeks...
September 20, 2017: Poultry Science
https://www.readbyqxmd.com/read/29048366/lipase-catalyzed-transesterification-of-egg-yolk-phophatidylcholine-with-concentrate-of-n-3-polyunsaturated-fatty-acids-from-cod-liver-oil
#16
Anna Chojnacka, Witold Gładkowski, Aleksandra Grudniewska
Phospholipids containing PUFAs are important vehicles for their delivering to the targeted tissues. In our research project we established enzymatic methods for the enrichment of natural egg-yolk PC with n-3 PUFAs. Instead of synthetic PUFA ethyl esters, the new strategy was developed using polyunsaturated fatty acids enriched fraction (PUFA-EF) from cod liver oil as the natural acyl donors. PUFA-EF was produced by urea-complexation and contained 86.9% PUFA including 8.5% stearidonic acid (SDA; 18:4(n-3)), 26...
October 19, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29045453/crispr-cas-mediated-knock-in-via-non-homologous-end-joining-in-the-crustacean-daphnia-magna
#17
Hitoshi Kumagai, Takashi Nakanishi, Tomoaki Matsuura, Yasuhiko Kato, Hajime Watanabe
The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated system (Cas) is widely used for mediating the knock-in of foreign DNA into the genomes of various organisms. Here, we report a process of CRISPR/Cas-mediated knock-in via non-homologous end joining by the direct injection of Cas9/gRNA ribonucleoproteins (RNPs) in the crustacean Daphnia magna, which is a model organism for studies on toxicology, ecology, and evolution. First, we confirmed the cleavage activity of Cas9 RNPs comprising purified Cas9 proteins and gRNAs in D...
2017: PloS One
https://www.readbyqxmd.com/read/29023530/allele-specific-expression-at-the-androgen-receptor-alpha-gene-in-a-hybrid-unisexual-fish-the-amazon-molly-poecilia-formosa
#18
Fangjun Zhu, Ingo Schlupp, Ralph Tiedemann
The all-female Amazon molly (Poecilia formosa) is the result of a hybridization of the Atlantic molly (P. mexicana) and the sailfin molly (P. latipinna) approximately 120,000 years ago. As a gynogenetic species, P. formosa needs to copulate with heterospecific males including males from one of its bisexual ancestral species. However, the sperm only triggers embryogenesis of the diploid eggs. The genetic information of the sperm donor typically will not contribute to the next generation of P. formosa. Hence, P...
2017: PloS One
https://www.readbyqxmd.com/read/29020571/factors-and-molecules-that-could-impact-cell-differentiation-in-the-embryo-generated-by-nuclear-transfer
#19
Renata Simões, Arnaldo Rodrigues Santos
Somatic cell nuclear transfer is a technique to create an embryo using an enucleated oocyte and a donor nucleus. Nucleus of somatic cells must be reprogrammed in order to participate in normal development within an enucleated egg. Reprogramming refers to the erasing and remodeling of cellular epigenetic marks to a lower differentiation state. Somatic nuclei must be reprogrammed by factors in the oocyte cytoplasm to a rather totipotent state since the reconstructed embryo must initiate embryo development from the one cell stage to term...
October 2, 2017: Organogenesis
https://www.readbyqxmd.com/read/28981550/from-mammals-back-to-birds-host-switch-of-the-acanthocephalan-corynosoma-australe-from-pinnipeds-to-the-magellanic-penguin-spheniscus-magellanicus
#20
Jesús Servando Hernández-Orts, Martha Brandão, Simona Georgieva, Juan Antonio Raga, Enrique Alberto Crespo, José Luis Luque, Francisco Javier Aznar
Trophically-transmitted parasites are regularly exposed to potential new hosts through food web interactions. Successful colonization, or switching, to novel hosts, occur readily when 'donor' and 'target' hosts are phylogenetically related, whereas switching between distantly related hosts is rare and may result from stochastic factors (i.e. rare favourable mutations). This study investigates a host-switching event between a marine acanthocephalan specific to pinnipeds that is apparently able to reproduce in Magellanic penguins Spheniscus magellanicus from Brazil...
2017: PloS One
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