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https://www.readbyqxmd.com/read/29145059/binding-of-naringin-and-naringenin-with-hen-egg-white-lysozyme-a-spectroscopic-investigation-and-molecular-docking-study
#1
Sourav Das, Pooja Ghosh, Sudipta Koley, Atanu Singha Roy
The interactions of naringenin (NG) and naringin (NR) with Hen Egg White Lysozyme (HEWL) in aqueous medium have been investigated using UV-vis spectroscopy, steady-state fluorescence, circular dichroism (CD), Fourier Transform infrared spectroscopy (FT-IR) and molecular docking analyses. Both NG and NR can quench the intrinsic fluorescence of HEWL via static quenching mechanism. At 300K, the value of binding constant (Kb) of HEWL-NG complex (5.596±0.063×10(4)M(-1)) was found to be greater than that of HEWL-NR complex (3...
November 8, 2017: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/29143944/one-thousand-seventy-eight-autologous-ivf-cycles-in-women-45%C3%A2-years-and-older-the-largest-single-center-cohort-to-date
#2
Vinay Gunnala, Mohamad Irani, Alexis Melnick, Zev Rosenwaks, Steven Spandorfer
PURPOSE: The purpose of this study was to determine IVF outcomes in women 45 years and older using autologous oocytes. METHODS: This is a retrospective cohort study reviewing all IVF cycles in women ≥ 45 years old from January 1995 to June 2015 that were conducted at one academic medical center. One thousand seventy-eight fresh, autologous IVF cycles met inclusion criteria. PGD/S, natural IVF, and donor egg cycles were excluded. Outcomes were analyzed for the different age groups (age 45, n = 773; age 46, n = 221; age 47, n = 57; age 48, n = 22; age 49, n = 5)...
November 16, 2017: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/29130972/human-dendritic-cells-with-th2-polarizing-capacity-analysis-using-label-free-quantitative-proteomics
#3
Leonie Hussaarts, Maria Mardalena Martini Kaisar, Arzu Tugce Guler, Hans Dalebout, Bart Everts, André M Deelder, Magnus Palmblad, Maria Yazdanbakhsh
BACKGROUND: Dendritic cells (DCs) are the sentinels of the immune system. Upon recognition of a pathogen, they mature and migrate to draining lymph nodes to prime and polarize T cell responses. Although it is known that helminths and helminth-derived molecules condition DCs to polarize T helper (Th) cells towards Th2, the underlying mechanisms remain incompletely understood. OBJECTIVES: The aim of this study was to conduct a proteome analysis of helminth antigen-stimulated DCs in order to gain more insight into the cellular processes associated with their ability to polarize immune responses...
November 10, 2017: International Archives of Allergy and Immunology
https://www.readbyqxmd.com/read/29117383/assisted-reproductive-technology-in-europe-2013-results-generated-from-european-registers-by-eshre
#4
C Calhaz-Jorge, C De Geyter, M S Kupka, J de Mouzon, K Erb, E Mocanu, T Motrenko, G Scaravelli, C Wyns, V Goossens
STUDY QUESTION: Are there any changes in the treatments involving ART and IUI initiated in Europe during 2013 compared with previous years? SUMMARY ANSWER: An increase in the overall number of ART cycles resulting from a higher number of countries reporting data was evident, the pregnancy rates (PRs) in 2013 remained stable compared with those reported in 2012, the number of transfers with multiple embryos (3+) was lower than ever before yet the multiple delivery rates (DRs) remained unchanged, and IUI activity and success rates were similar to those of last years...
October 1, 2017: Human Reproduction
https://www.readbyqxmd.com/read/29116706/effect-of-sperm-dna-fragmentation-on-embryo-development-clinical-and-biological-aspects
#5
Cristian Alvarez Sedó, Melina Bilinski, Daniela Lorenzi, Heydy Uriondo, Felicitas Noblía, Valeria Longobucco, Estefanía Ventimiglia Lagar, Florencia Nodar
OBJECTIVE: The aim of this study was to investigate the effect of sperm DNA fragmentation on fertilization rate, embryo development (blastulation rate), and pregnancy outcomes for ICSI cycles performed in a cohort of couples using donor eggs and to assess the remaining embryos that were not transferred or frozen for apoptotic markers. METHODS: Eighty-two women (egg recipients) were included in the study (2016) were included in the study. The recipients' mean age was 41...
November 8, 2017: JBRA Assisted Reproduction
https://www.readbyqxmd.com/read/29070706/does-egg-donation-for-mitochondrial-replacement-techniques-generate-parental-responsibilities
#6
César Palacios-González
Children created through mitochondrial replacement techniques (MRTs) are commonly presented as possessing 50% of their mother's nuclear DNA, 50% of their father's nuclear DNA and the mitochondrial DNA of an egg donor. This lab-engineered genetic composition has prompted two questions: Do children who are the product of an MRT procedure have three genetic parents? And, do MRT egg donors have parental responsibilities for the children created? In this paper, I address the second question and in doing so I also address the first one...
October 25, 2017: Journal of Medical Ethics
https://www.readbyqxmd.com/read/29053854/dietary-betaine-supplementation-increases-adrenal-expression-of-steroidogenic-acute-regulatory-protein-and-yolk-deposition-of-corticosterone-in-laying-hens
#7
Halima Abobaker, Yun Hu, Zhen Hou, Qinwei Sun, Abdulrahman A Idriss, Nagmeldin A Omer, Yibo Zong, Ruqian Zhao
Betaine, an important methyl donor, is known to execute epigenetic regulation of gene expression via nutritional reprogramming. Herein, we explore whether feeding a betaine-supplemented diet to laying hens would affect corticosteroid biosynthesis in the adrenal gland and corticosterone deposition in eggs, in correlation with the expression of methyl transfer enzymes and the promoter DNA methylation status of affected genes. Rugao yellow-feathered laying hens at 38 weeks of age were assigned to Control and Betaine groups, fed basal and betaine-supplemented diets, respectively, for four weeks...
September 20, 2017: Poultry Science
https://www.readbyqxmd.com/read/29048366/lipase-catalyzed-transesterification-of-egg-yolk-phophatidylcholine-with-concentrate-of-n-3-polyunsaturated-fatty-acids-from-cod-liver-oil
#8
Anna Chojnacka, Witold Gładkowski, Aleksandra Grudniewska
Phospholipids containing PUFAs are important vehicles for their delivering to the targeted tissues. In our research project we established enzymatic methods for the enrichment of natural egg-yolk PC with n-3 PUFAs. Instead of synthetic PUFA ethyl esters, the new strategy was developed using polyunsaturated fatty acids enriched fraction (PUFA-EF) from cod liver oil as the natural acyl donors. PUFA-EF was produced by urea-complexation and contained 86.9% PUFA including 8.5% stearidonic acid (SDA; 18:4(n-3)), 26...
October 19, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29045453/crispr-cas-mediated-knock-in-via-non-homologous-end-joining-in-the-crustacean-daphnia-magna
#9
Hitoshi Kumagai, Takashi Nakanishi, Tomoaki Matsuura, Yasuhiko Kato, Hajime Watanabe
The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated system (Cas) is widely used for mediating the knock-in of foreign DNA into the genomes of various organisms. Here, we report a process of CRISPR/Cas-mediated knock-in via non-homologous end joining by the direct injection of Cas9/gRNA ribonucleoproteins (RNPs) in the crustacean Daphnia magna, which is a model organism for studies on toxicology, ecology, and evolution. First, we confirmed the cleavage activity of Cas9 RNPs comprising purified Cas9 proteins and gRNAs in D...
2017: PloS One
https://www.readbyqxmd.com/read/29023530/allele-specific-expression-at-the-androgen-receptor-alpha-gene-in-a-hybrid-unisexual-fish-the-amazon-molly-poecilia-formosa
#10
Fangjun Zhu, Ingo Schlupp, Ralph Tiedemann
The all-female Amazon molly (Poecilia formosa) is the result of a hybridization of the Atlantic molly (P. mexicana) and the sailfin molly (P. latipinna) approximately 120,000 years ago. As a gynogenetic species, P. formosa needs to copulate with heterospecific males including males from one of its bisexual ancestral species. However, the sperm only triggers embryogenesis of the diploid eggs. The genetic information of the sperm donor typically will not contribute to the next generation of P. formosa. Hence, P...
2017: PloS One
https://www.readbyqxmd.com/read/29020571/factors-and-molecules-that-could-impact-cell-differentiation-in-the-embryo-generated-by-nuclear-transfer
#11
Renata Simões, Arnaldo Rodrigues Santos
Somatic cell nuclear transfer is a technique to create an embryo using an enucleated oocyte and a donor nucleus. Nucleus of somatic cells must be reprogrammed in order to participate in normal development within an enucleated egg. Reprogramming refers to the erasing and remodeling of cellular epigenetic marks to a lower differentiation state. Somatic nuclei must be reprogrammed by factors in the oocyte cytoplasm to a rather totipotent state since the reconstructed embryo must initiate embryo development from the one cell stage to term...
October 2, 2017: Organogenesis
https://www.readbyqxmd.com/read/28981550/from-mammals-back-to-birds-host-switch-of-the-acanthocephalan-corynosoma-australe-from-pinnipeds-to-the-magellanic-penguin-spheniscus-magellanicus
#12
Jesús Servando Hernández-Orts, Martha Brandão, Simona Georgieva, Juan Antonio Raga, Enrique Alberto Crespo, José Luis Luque, Francisco Javier Aznar
Trophically-transmitted parasites are regularly exposed to potential new hosts through food web interactions. Successful colonization, or switching, to novel hosts, occur readily when 'donor' and 'target' hosts are phylogenetically related, whereas switching between distantly related hosts is rare and may result from stochastic factors (i.e. rare favourable mutations). This study investigates a host-switching event between a marine acanthocephalan specific to pinnipeds that is apparently able to reproduce in Magellanic penguins Spheniscus magellanicus from Brazil...
2017: PloS One
https://www.readbyqxmd.com/read/28974308/embryo-cryopreservation-and-preeclampsia-risk
#13
Cynthia K Sites, Donna Wilson, Maya Barsky, Dana Bernson, Ira M Bernstein, Sheree Boulet, Yujia Zhang
OBJECTIVE: To determine whether assisted reproductive technology (ART) cycles involving cryopreserved-warmed embryos are associated with the development of preeclampsia. DESIGN: Retrospective cohort study. SETTING: IVF clinics and hospitals. PATIENT(S): A total of 15,937 births from ART: 9,417 singleton and 6,520 twin. INTERVENTION(S): We used linked ART surveillance, birth certificate, and maternal hospitalization discharge data, considering resident singleton and twin births from autologous or donor eggs from 2005-2010...
November 2017: Fertility and Sterility
https://www.readbyqxmd.com/read/28972428/sperm-dna-fragmentation-index-does-not-correlate-with-blastocyst-euploidy-rate-in-egg-donor-cycles
#14
Itai Gat, Nancy Li, Natalia Yasovich, Ran Antes, Valeriy Kuznyetsov, Khaled Zohni, Noga Fuchs Weizman, Clifford Librach
Sperm DNA fragmentation is a known etiology for male infertility. We evaluated the impact of sperm DNA fragmentation index (DFI) on blastocyst euploidy in IVF cycles with egg donors. This observational retrospective study, which was conducted in a university affiliated fertility clinic, included IVF-ICSI-pre-implantation Genetic Screening (PGS) egg donor cycles in which DFI was tested prior to IVF, between January 1st, 2014 and July 31st, 2016. Twenty-seven cycles with DFI > 15% were included in the study group and compared with 18 cycles of DFI < 15% within control group...
October 3, 2017: Gynecological Endocrinology
https://www.readbyqxmd.com/read/28942506/production-of-tiger-puffer-takifugu-rubripes-offspring-from-triploid-grass-puffer-takifugu-niphobles-parents
#15
Masaomi Hamasaki, Yutaka Takeuchi, Ryosuke Yazawa, Souta Yoshikawa, Kazushi Kadomura, Toshiyuki Yamada, Kadoo Miyaki, Kiyoshi Kikuchi, Goro Yoshizaki
The tiger puffer Takifugu rubripes is one of the most popular aquacultural fish; however, there are two major obstacles to selective breeding. First, they have a long generation time of 2 or 3 years until maturation. Second, the parental tiger puffer has a body size (2-5 kg) much larger than average market size (0.6-1.0 kg). The grass puffer Takifugu niphobles is closely related to the tiger puffer and matures in half the time. Furthermore, grass puffer can be reared in small areas since their maturation weight is about 1/150 that of mature tiger puffer...
September 23, 2017: Marine Biotechnology
https://www.readbyqxmd.com/read/28929788/the-familial-and-the-familiar-locating-relatedness-in-colombian-donor-conception
#16
Malissa Kay Shaw
In this article, I explore how women undergoing in-vitro fertilization with familial or anonymous egg donors located relatedness with a donor-conceived child through familial and social identities. Recognizing gametes as substances that contain biological and sociocultural/behavioral traits, shaped women's narratives around interconnected notions of the familial and familiar, or the social understanding of biological and social inheritance, and knowledge of the genetic materials involved. Women's narratives of relatedness reflect their relationships with family and society and their desire to reproduce these relationships in their child(ren), a process that reproduces prevailing Colombian social values and notions of ideal citizens...
September 20, 2017: Medical Anthropology
https://www.readbyqxmd.com/read/28923287/aberrant-expression-of-epithelial-leucine-rich-repeat-containing-g-protein-coupled-receptor-5-positive-cells-in-the-eutopic-endometrium-in-endometriosis-and-implications-in-deep-infiltrating-endometriosis
#17
Júlia Vallvé-Juanico, Elena Suárez-Salvador, Josep Castellví, Agustín Ballesteros, Hugh S Taylor, Antonio Gil-Moreno, Xavier Santamaria
OBJECTIVE: To characterize leucine-rich repeat containing G protein-coupled receptor 5-positive (LGR5(+)) cells from the endometrium of women with endometriosis. DESIGN: Prospective experimental study. SETTING: University hospital/fertility clinic. PATIENT(S): Twenty-seven women with endometriosis who underwent surgery and 12 healthy egg donors, together comprising 39 endometrial samples. INTERVENTION(S): Obtaining of uterine aspirates by using a Cornier Pipelle...
November 2017: Fertility and Sterility
https://www.readbyqxmd.com/read/28901845/a-review-of-patents-on-therapeutic-potential-and-delivery-of-hydrogen-sulfide
#18
Richa Verma, Yumna Akhtar, Somnath Singh
Hydrogen sulfide (H2S) is a colorless gas with a characteristic smell of rotten eggs. Once only thought of as a toxic gas, evidence now shows that H2S plays major roles in pathological and physiological activities. These roles are being utilized to treat diseases and disorders ranging from hypertension, inflammation, edema, cardiovascular issues, chronic pain, cancer, and many more. Challenges facing the use of H2S currently involve achieving the optimum therapeutic concentrations, synthesizing chemically and physiologically stable donors, and developing clinically appropriate delivery systems...
September 11, 2017: Recent Patents on Drug Delivery & Formulation
https://www.readbyqxmd.com/read/28889935/sociocultural-determinants-of-us-women-s-ethical-views-on-various-fertility-treatments
#19
Stephen C Collins, Esther Chan
Ethical concerns over treatments for infertility can discourage patients from pursuing fertility healthcare. This study aims to evaluate the sociocultural factors that influence the ethical views of reproductive-aged women regarding various fertility treatments. A publicly available cross-sectional survey of 4792 nationally representative US women aged 25-52 years was analysed to identify the frequency of ethical concerns over such interventions. Concerns were most common for treatments that increase the chance of twins (54%), followed by third-party reproductive strategies (48-51%), IVF (30%) and partner insemination (14%)...
August 25, 2017: Reproductive Biomedicine Online
https://www.readbyqxmd.com/read/28822010/vitrification-of-human-immature-oocytes-before-and-after-in-vitro-maturation-a-review
#20
REVIEW
Mohammad Ali Khalili, Abbas Shahedi, Sareh Ashourzadeh, Stefania Annarita Nottola, Guido Macchiarelli, Maria Grazia Palmerini
The use of immature oocytes subjected to in vitro maturation (IVM) opens interesting perspectives for fertility preservation where ovarian reserves are damaged by pathologies or therapies, as in PCO/PCOS and cancer patients. Human oocyte cryopreservation may offer some advantages compared to embryo freezing, such as fertility preservation in women at risk of losing fertility due to oncological treatment or chronic disease, egg donation and postponing childbirth. It also eliminates religious and/or other ethical, legal, and moral concerns of embryo freezing...
August 18, 2017: Journal of Assisted Reproduction and Genetics
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