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https://www.readbyqxmd.com/read/29678568/likelihood-of-pregnancy-after-the-transfer-of-embryos-derived-from-follicle-aspiration-and-in-vitro-embryo-production-sessions-with-different-relative-efficiencies
#1
Luiz Fernando Feres, Luiz Gustavo Bruno Siqueira, Miller Pereira Palhao, Lívia Loiola Dos Santos, Felipe Zandonadi Brandao, Joao Henrique Moreira Viana
The aim of the present study was to evaluate the likelihood of pregnancy of in vitro-produced (IVP) embryos from batches with distinct relative efficiencies. Data were retrospectively analyzed from 605 transvaginal ultrasonic-guided follicle aspiration sessions (OPU) followed by in vitro embryo production (IVEP) and 2456 fresh embryo transfers (ET), performed between 2008 and 2012 in individuals of the Gir (dairy Bos indicus) breed. The OPU and IVEP were performed using standard procedures by a single group of technicians at the same laboratory facility...
April 15, 2018: Animal Reproduction Science
https://www.readbyqxmd.com/read/29673730/early-oocyte-denudation-does-not-compromise-icsi-cycle-outcome-a-large-retrospective-cohort-study
#2
Osama Naji, Natalie Moska, Yaser Dajani, Awatuf El-Shirif, Hassan El-Ashkar, Mohamed Hosni, Mohamed Khalil, Yacoub Khalaf, Virginia Bolton, Tarek El-Toukhy
This retrospective cohort study of 2051 consecutive fresh non-donor intracytoplasmic sperm injection (ICSI) cycles investigated whether time from oocyte retrieval to denudation, precisely measured and recorded by an operator-independent automated radiofrequency-based system, affected cycle outcome. ICSI cycles were divided into two groups: group I (denudation within <2 h of oocyte retrieval, n = 1118) and group II (denudation 2-5 h after oocyte retrieval, n = 933). Univariate analysis by two-sample t-test or Mann-Whitney test was used, as appropriate...
April 4, 2018: Reproductive Biomedicine Online
https://www.readbyqxmd.com/read/29625802/cryosurvival-of-rabbit-embryos-obtained-after-superovulation-with-corifollitropin-alfa-with-or-without-lh
#3
José Salvador Vicente, María Pilar Viudes-de-Castro, José Isaí Cedano-Castro, Francisco Marco-Jiménez
The efficiency of an embryo bank depends on provision of optimal conditions for recovery, cryopreservation and transfer to a breed or strain. In this sense, increasing the number of embryos available using superovulation should improve the cryobank efficiency. However, vagueness of response to conventional protocols to control or increase ovarian response and the quality of oocytes and embryos and their cryotolerance remain a challenge. The aim of our study was to evaluate the effect of corifollitropin alpha (CTP) and a recombinant human FSH (rhFSH), alone or supplemented with rhLH, on embryo cryosurvival by in vitro development and OCT4 and NANOG mRNA abundance at blastocyst stage and offspring rate...
March 31, 2018: Animal Reproduction Science
https://www.readbyqxmd.com/read/29624006/intrinsic-and-extrinsic-molecular-determinants-or-modulators-for-epigenetic-remodeling-and-reprogramming-of-somatic-cell-derived-genome-in-mammalian-nuclear-transferred-oocytes-and-resultant-embryos
#4
M Samiec, M Skrzyszowska
The efficiency of somatic cell cloning in mammals remains disappointingly low. Incomplete and aberrant reprogramming of epigenetic memory of somatic cell nuclei in preimplantation nuclear- transferred (NT) embryos is one of the most important factors that limit the cloning effectiveness. The extent of epigenetic genome-wide alterations, involving histone or DNA methylation and histone deacetylation, that are mediated by histone-lysine methyltransferases (HMTs) or DNA methyltransferases (DNMTs) and histone deacetylases (HDACs) can be modulated/reversed via exogenous inhibitors of these enzymes throughout in vitro culture of nuclear donor cells, nuclear recipient oocytes and/or cloned embryos...
March 2018: Polish Journal of Veterinary Sciences
https://www.readbyqxmd.com/read/29622087/generation-of-venus-fluorochrome-expressing-transgenic-handmade-cloned-buffalo-embryos-using-sleeping-beauty-transposon
#5
Dharmendra Kumar, Papori Sharma, Kennady Vijayalakshmy, Naresh L Selokar, Pradeep Kumar, Rasika Rajendran, P S Yadav
The objective of this study was to optimise the electroporation conditions for efficient integration of Venus construct in buffalo fetal fibroblasts using Sleeping Beauty (SB) based transposition and to produce Venus expressing transgenic cloned embryos through handmade cloning (HMC) approach. Primary culture of buffalo fetal fibroblast cells was established and subsequently cultured cells were co-transfected with Venus and helper plasmid at different combinations of electroporation condition. In different combinations of voltage, time and plasmid dose, we observed that 300 V, single pulse for 10 ms in 2 mm cuvette and 1...
April 2018: Tissue & Cell
https://www.readbyqxmd.com/read/29611308/factors-impacting-couples-decision-making-between-vasectomy-reversal-versus-sperm-retrieval-in-vitro-fertilization-intracytoplasmic-sperm-injection
#6
P K Kavoussi, K M Kavoussi, K C Odenwald, S K Kavoussi
The purpose of this study was to identify factors which impact a couples' decision-making between the options of vasectomy reversal vs. sperm retrieval/in vitro fertilization/intracytoplasmic sperm injection when counseled both by a reproductive urologist and a reproductive endocrinologist. A retrospective chart review was performed of couples who wish to achieve a pregnancy with a male partner with a history of prior vasectomy, in a couples' private fertility center. Of patients presenting for fertility options with a history of vasectomy, 175 couples elected to be counseled by both a reproductive urologist and a reproductive endocrinologist on the options between vasectomy reversal and sperm retrieval/in vitro fertilization/intracytoplasmic sperm injection, with 78...
April 2, 2018: Andrology
https://www.readbyqxmd.com/read/29609956/the-arcuate-uterus-is-there-an-impact-on-in-vitro-fertilization-outcomes-after-euploid-embryo-transfer
#7
Eric S Surrey, Mandy Katz-Jaffe, Rebecca L Surrey, Amy Snyder Small, Robert L Gustofson, William B Schoolcraft
OBJECTIVE: To study the impact of the arcuate uterus on euploid blastocyst-stage embryo transfer outcomes after comprehensive chromosomal screening (CCS). DESIGN: Controlled retrospective trial. SETTING: Tertiary care assisted reproduction technology (ART) center. PATIENT(S): Consecutive patients undergoing in vitro fertilization and euploid embryo transfer after CCS during 2014. INTERVENTION(S): Ultrasound examinations and office hysteroscopy; array comparative genomic hybridization to perform CCS after a trophectoderm biopsy...
March 30, 2018: Fertility and Sterility
https://www.readbyqxmd.com/read/29605176/preventing-mitochondrial-diseases-embryo-sparing-donor-independent-options
#8
REVIEW
Eli Y Adashi, I Glenn Cohen
Mutant mitochondrial DNA gives rise to a broad range of incurable inborn maladies. Prevention may now be possible by replacing the mutation-carrying mitochondria of zygotes or oocytes at risk with donated unaffected counterparts. However, mitochondrial replacement therapy is being held back by theological, ethical, and safety concerns over the loss of human zygotes and the involvement of a donor. These concerns make it plain that the identification, validation, and regulatory adjudication of novel embryo-sparing donor-independent technologies remains a pressing imperative...
March 28, 2018: Trends in Molecular Medicine
https://www.readbyqxmd.com/read/29603433/supplementation-with-sunflower-seeds-in-beef-cattle-did-not-impact-on-oocyte-and-in-vitro-embryo-production
#9
A L Baltazar, G M de Mattos, B M Ropelli, Smg Firetti, C Castilho, G Pugliesi, Mbc Maldonado, M Binelli, Jof Silva, G C Lupatini, B S Lafuente, Cmb Membrive
Supplementation with compounds rich in linoleic acid, including sunflower seed supplementation, promotes increase in conception rates in cows. We aimed to evaluate whether the sunflower seed (linoleic acid source) supplementation in beef donor females alters the plasma concentrations of cholesterol, triglycerides, HDL and LDL, increases the number and quality of oocytes, increases the cleavage rates and determines an improvement in number and quality of in vitro produced blastocysts. Thus, Nelore females were divided into two groups of 15 animals to receive supplementation with or without sunflower seed for 57 days...
March 30, 2018: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/29599204/late-effects-after-hematopoietic-stem-cell-transplantation-for-beta-thalassemia-major-the-french-national-experience
#10
Ilhem Rahal, Claire Galambrun, Yves Bertrand, Nathalie Garnier, Catherine Paillard, Pierre Frange, Corinne Pondarré, Jean Hugues Dalle, Regis Peffault de Latour, Mauricette Michallet, Dominique Steschenko, Despina Moshous, Patrick Lutz, Jean Louis Stephan, Pierre Simon Rohrlich, Ibrahim Yakoub-Agha, Francoise Bernaudin, Christophe Piguet, Nathalie Aladjidi, Catherine Badens, Claire Berger, Gérard Socié, Cécile Dumesnil, Marie Pierre Castex, Marilyne Poirée, Anne Lambilliotte, Caroline Thomas, Pauline Simon, Pascal Auquier, Gerard Michel, Anderson Loundou, Imane Agouti, Isabelle Thuret
In this retrospective study, we evaluate long-term complications in nearly all β-thalassemia-major patients who successfully received, in France, allogeneic hematopoietic stem cell transplantation. 99 patients were analysed with a median age of 5.9 years at transplantation. The median duration of clinical follow-up was 12 years. All conditioning regimen were myeloablative, most often based on busulfan combined with cyclophosphamide and more than 90% of patients underwent a transplant from a matched sibling donor...
March 29, 2018: Haematologica
https://www.readbyqxmd.com/read/29549827/variations-in-bovine-embryo-production-between-individual-donors-for-opu-ivf-are-closely-related-to-glutathione-concentrations-in-oocytes-during-in-vitro-maturation
#11
Takemasa Hidaka, Yutaka Fukumoto, Satoshi Yamamoto, Yasuhiro Ogata, Toshitaka Horiuchi
Variations in embryo production between individual oocyte donors represent a serious problem in cattle production, when implementing ovum pick-up (OPU) and in vitro maturation (IVM) of oocytes. However, the precise cause of this problem is unknown. Here, we aimed to investigate the relationship between the glutathione (GSH) concentration in IVM oocytes and embryo development to explore a potential cause of the variations between individual donors. First, we found a high positive correlation (r = 0.80) between the GSH concentration in IVM oocytes and the blastocyst development rate of oocytes collected in the same OPU session for each donor (N = 11)...
March 10, 2018: Theriogenology
https://www.readbyqxmd.com/read/29545772/a-proof-of-concept-clinical-trial-of-a-single-luteal-use-of-long-acting-gonadotropin-releasing-hormone-antagonist-degarelix-in-controlled-ovarian-stimulation-for-in-vitro-fertilization-long-antagonist-protocol
#12
Evangelos G Papanikolaou, Hakan Yarali, Evi Timotheou, Michael Grynberg, Odysseas Zafeiratis, Herman Tournaye, Robert Najdecki
Introduction: A drawback of gonadotropin-releasing hormone (GnRH) antagonist protocols in in vitro fertilization (IVF) is that they have limited flexibility in cycle programming. This proof of concept study explored the efficacy of a single-dose, long-acting GnRH antagonist IVF protocol. Trial registration number is NCT03240159, retrospectively registered on March 08, 2017. Materials and methods: The efficacy of a single-dose long-acting antagonist, degarelix, was explored initially in healthy donors and subsequently in infertile patients...
2018: Frontiers in Endocrinology
https://www.readbyqxmd.com/read/29542856/delay-in-maternal-transcript-degradation-in-ovine-embryos-derived-from-low-competence-oocytes
#13
Laura Masala, Federica Ariu, Luisa Bogliolo, Emanuela Bellu, Sergio Ledda, Daniela Bebbere
Oocytes from prepubertal animals have a reduced ability to undergo embryo development and produce viable offspring. The present work used an ovine model consisting of oocytes derived from adult and prepubertal donors to assess the molecular status of oocytes and preimplantation embryos with different developmental competence. The lower potential of oocytes of young donors was confirmed in terms of in vitro developmental capabilities and kinetics. A panel of genes including maternal effect (DPPA3, GDF9, NMP2, ZAR1) and housekeeping genes (ACTB, RPL19, SDHA, YWHAZ, ATP1A1), genes involved in DNA methylation (DNMT1, DNMT3A, DNMT3B), genomic imprinting (IGF2R), pluripotency (NANOG, POU5F1) and cell cycle regulation (CCNB1, CDK1, MELK) was relatively quantified...
March 15, 2018: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/29534148/preterm-birth-and-small-for-gestational-age-in-singleton-in-vitro-fertilization-births-using-donor-oocytes
#14
Sheree L Boulet, Jennifer F Kawwass, Sara Crawford, Michael J Davies, Dmitry M Kissin
We used 2006-2015 US National Assisted Reproductive Technology Surveillance System data to compare preterm birth and fetal growth for livebo n singletons (24-42 week gestation) following donor versus autologous oocyte in vitro fertilization (IVF). Using binary and multinomial logistic regressi n, we computed adjusted odds ratios (aORs) and 95% confidence intervals (CIs) for associations between use of donor oocytes and preterm delivery, small-f r-gestational-age (SGA), and large-for-gestational age (LGA), stratified by fresh and thawed embryo status and accounting for maternal characteristics and year of birth...
March 9, 2018: American Journal of Epidemiology
https://www.readbyqxmd.com/read/29510633/-medroxyprogesteron-acetate-use-to-block-lh-surge-in-oocyte-donor-stimulation
#15
I Crha, P Ventruba, E Filipinská, M Dziakova, J Žáková, M Ješeta, E Lousová, Z Papíková
OBJECTIVE: To compare the results of ovarian stimulation with LH surge blockade by medroxyprogesterone acetate or GnRH antagonist in oocytes donors. To present current options of exogenous and endogenous progestins instead of GnRH analogues to block LH surge during ovarian stimulation. DESIGN: Retrospective study of oocyte donor cycles and literature review. SETTING: Department of Obstetrics and Gynecology, Faculty of Medicine, Masaryk university and University Hospital Brno...
2018: Ceská Gynekologie
https://www.readbyqxmd.com/read/29473888/melatonin-mediated-development-of-ovine-cumulus-cells-perhaps-by-regulation-of-dna-methylation
#16
Yi Fang, Shoulong Deng, Jinlong Zhang, Haijun Liu, Yihai Li, Xiaosheng Zhang, Yixun Liu
Cumulus cells of pre-pubertal domestic animals are dysfunctional, perhaps due to age-specific epigenetic events. This study was designed to determine effects of melatonin treatment of donors on methylation modification of pre-pubertal cumulus cells. Cumulus cells from germinal vesicle stage cumulus oocyte complexes (COCs) were collected from eighteen lambs which were randomly divided into control group (C) and melatonin group given an 18 mg melatonin implant subcutaneous (M). Compared to the C group, the M group had higher concentrations of melatonin in plasma and follicular fluid ( p < 0...
February 23, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29432583/the-clinical-utility-of-pgd-with-hla-matching-a-collaborative-multi-centre-eshre-study
#17
G Kakourou, S Kahraman, G C Ekmekci, H A Tac, G Kourlaba, E Kourkouni, A Cervero Sanz, J Martin, H Malmgren, C Giménez, V Gold, F Carvalho, C Billi, J F C Chow, X Vendrell, G Kokkali, J Liss, J Steffann, J Traeger-Synodinos
STUDY QUESTION: Has PGD-HLA been successful relative to diagnostic and clinical efficacy? SUMMARY ANSWER: The diagnostic efficacy of PGD-HLA protocols was found lower in this study in comparison to published PGD-HLA protocols and to that reported for general PGD by ESHRE (78.5 vs 94.1% and vs 92.6%, respectively), while the clinical efficacy has proven very difficult to assess due to inadequate follow-up of both the ART/PGD and HSCT procedure outcomes. WHAT IS KNOWN ALREADY: The first clinical cases for PGD-HLA were reported in 2001...
February 8, 2018: Human Reproduction
https://www.readbyqxmd.com/read/29428314/gestational-carrier-in-assisted-reproductive-technology
#18
Gayathree Murugappan, Leslie V Farland, Stacey A Missmer, Katharine F Correia, Raymond M Anchan, Elizabeth S Ginsburg
OBJECTIVE: To compare clinical outcomes of in vitro fertilization (IVF) cycles with the use of gestational carriers (GCs) with non-GC IVF cycles. DESIGN: Retrospective cohort study of assisted reproductive technology (ART) cycles performed with (24,269) and without (1,313,452) the use of a GC. SETTING: ART centers. PATIENT(S): Infertile patients seeking IVF with or without use of a GC. INTERVENTIONS(S): Autologous and donor oocyte cycles, fresh and cryopreserved embryo transfer cycles...
February 7, 2018: Fertility and Sterility
https://www.readbyqxmd.com/read/29395327/cloning-of-macaque-monkeys-by-somatic-cell-nuclear-transfer
#19
Zhen Liu, Yijun Cai, Yan Wang, Yanhong Nie, Chenchen Zhang, Yuting Xu, Xiaotong Zhang, Yong Lu, Zhanyang Wang, Muming Poo, Qiang Sun
Generation of genetically uniform non-human primates may help to establish animal models for primate biology and biomedical research. In this study, we have successfully cloned cynomolgus monkeys (Macaca fascicularis) by somatic cell nuclear transfer (SCNT). We found that injection of H3K9me3 demethylase Kdm4d mRNA and treatment with histone deacetylase inhibitor trichostatin A at one-cell stage following SCNT greatly improved blastocyst development and pregnancy rate of transplanted SCNT embryos in surrogate monkeys...
February 8, 2018: Cell
https://www.readbyqxmd.com/read/29371826/long-term-clinical-outcomes-of-testicular-sperm-extraction-and-intracytoplasmic-sperm-injection-for-infertile-men
#20
Noriyuki Okuyama, Ryuichiro Obata, Nao Oka, Yusuke Nakamura, Hiromitsu Hattori, Yukiko Nakajo, Nobuya Aono, Masae Koizumi, Mayumi Toya, Koichi Nagao, Toshihiro Tai, Tomoko Hashimoto, Hideki Igarashi, Koichi Kyono
Purpose: To find the best methods to achieve the highest pregnancy and birth rates for couples needing testicular sperm extraction (TESE)-intracytoplasmic sperm injection (ICSI). Methods: Retrospectively studied were 801 patients with male factor infertility who had undergone TESE-ICSI between April, 1996 and July, 2016 and who had been categorized into four groups: obstructive azoospermia (OA); non-obstructive azoospermia (NOA); Klinefelter syndrome (KS); and cryptozoospermia (Crypt)...
January 2018: Reproductive Medicine and Biology
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