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https://www.readbyqxmd.com/read/28822904/the-effect-of-age-and-length-of-gonadotropin-stimulation-on-the-in%C3%A2-vitro-embryo-development-of-holstein-calf-oocytes
#1
Luke Currin, Laura Michalovic, Anne-Marie Bellefleur, Karina Gutierrez, Werner Glanzner, Yasmin Schuermann, Rodrigo C Bohrer, Naomi Dicks, Paulo R da Rosa, Matheus P De Cesaro, Rosalba Lopez, François-Xavier Grand, Christian Vigneault, Patrick Blondin, Jim Gourdon, Hernan Baldassarre, Vilceu Bordignon
The use of oocytes recovered from prepubertal donors for in vitro embryo production has great potential for accelerating the rate of genetic gain in the dairy industry. However, these oocytes are known to be less developmentally competent than those from adult donors. In this study, we investigated the effect of age and gonadotropin stimulation in Holstein heifers subjected to oocyte collection every two weeks between 2 and 6 months of age. In order to assess the effect of gonadotropin stimulation, animals were subjected to one of three treatments, namely Short (ST; 36-42 h), Long (LT; ≥72 h) and No Treatment (NT) prior to laparoscopic ovum pick-up (LOPU)...
August 10, 2017: Theriogenology
https://www.readbyqxmd.com/read/28822010/vitrification-of-human-immature-oocytes-before-and-after-in-vitro-maturation-a-review
#2
REVIEW
Mohammad Ali Khalili, Abbas Shahedi, Sareh Ashourzadeh, Stefania Annarita Nottola, Guido Macchiarelli, Maria Grazia Palmerini
The use of immature oocytes subjected to in vitro maturation (IVM) opens interesting perspectives for fertility preservation where ovarian reserves are damaged by pathologies or therapies, as in PCO/PCOS and cancer patients. Human oocyte cryopreservation may offer some advantages compared to embryo freezing, such as fertility preservation in women at risk of losing fertility due to oncological treatment or chronic disease, egg donation and postponing childbirth. It also eliminates religious and/or other ethical, legal, and moral concerns of embryo freezing...
August 18, 2017: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/28815355/egg-donation-of-vitrified-oocytes-bank-produces-similar-pregnancy-rates-by-blastocyst-transfer-when-compared-to-fresh-cycle
#3
Thais S Domingues, Ana Paula Aquino, Bruna Barros, Raquel Mazetto, Mariana Nicolielo, Carolina M Kimati, Talita Devecchi, Tatiana C S Bonetti, Paulo C Serafini, Eduardo L A Motta
PURPOSE: Advances in reproductive techniques, mainly the introduction of oocyte vitrification, have provided the opportunity to conceive from oocyte banks. The aim of this study was to compare the clinical outcomes of fresh and vitrified oocytes in an egg donation program following blastocyst transfer. METHODS: This retrospective observational study included 504 oocyte donation cycles. All donor women were younger than 30 years of age. The recipient cycles were divided into two groups: fresh oocytes (n = 78) or vitrified oocytes (n = 426)...
August 16, 2017: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/28797743/importance-of-a-5-versus-7-day-pill-free-interval-in-a-gnrh-antagonist-protocol-using-corifollitropin-alfa-a-prospective-cohort-study-in-oocyte-donors
#4
Alicia Pérez-Calvo, Francisca Martínez, Christophe Blockeel, Elisabeth Clúa, Ignacio Rodríguez, Pere N Barri, Buenaventura Coroleu
In this prospective cohort study, oocyte donors were recruited prospectively and assigned to receive corifollitropin alfa: 5 days after pill discontinuation (group D5; 42 donors), or 7 days after pill discontinuation (group D7; 50 donors) in a gonadotrophin-releasing hormone antagonist protocol. Fixed additional daily doses of 200 IU recombinent FSH (rFSH) were started after 7 days of corifollitropinalfa, until triggering. No significant differences in basal characteristics were observed between both groups...
July 14, 2017: Reproductive Biomedicine Online
https://www.readbyqxmd.com/read/28796907/likelihood-of-pregnancy-after-embryo-transfer-is-reduced-in-recipient-mares-with-a-short-preceding-oestrus
#5
J Cuervo-Arango, A N Claes, M Ruijter-Villani, T A Stout
BACKGROUND: Previous surveys reported a positive association between the length of the follicular phase and subsequent fertility in embryo transfer donor and Thoroughbred mares. However, it is unclear whether a longer oestrus positively influences fertilisation and oviductal development (oocyte quality, oviductal environment), or uterine receptivity and survival of the embryo in the uterus. OBJECTIVES: To determine the effect of length of oestrus (characterised by duration of endometrial oedema) on likelihood of pregnancy and early embryo loss (EEL) in recipient mares after embryo transfer (ET)...
August 10, 2017: Equine Veterinary Journal
https://www.readbyqxmd.com/read/28795051/predictors-of-live-birth-and-pregnancy-success-after-in-vitro-fertilization-in-infertile-women-aged-40-and-over
#6
Hye Ok Kim, Nayoung Sung, In Ok Song
OBJECTIVE: The aim of this study was to evaluate pregnancy outcomes and the live birth rate at 1-year age increments in women aged ≥40 years undergoing fresh non-donor in vitro fertilization (IVF) and embryo transfer (ET), and to identify predictors of success in these patients. METHODS: This retrospective study was performed among women ≥40 years of age between 2004 and 2011. Of the 2,362 cycles that were conducted, ET was performed in 1,532 (73.1%). RESULTS: The clinical pregnancy rate and live birth rate in women ≥40 years significantly decreased with each year of increased age (p<0...
June 2017: Clinical and Experimental Reproductive Medicine
https://www.readbyqxmd.com/read/28774548/safety-and-efficiency-of-oocyte-vitrification
#7
REVIEW
Neelke De Munck, Gábor Vajta
As the oocyte is the starting point for a new life, artificial reproductive technology (ART) techniques should not affect the (ultra) structural and functional integrity, or the developmental competence. Oocyte vitrification -one of the most significant achievements in human ART during the past decade- should therefore be a safe and efficient technique. This review discusses the principles and developments of the existing and future techniques, applications possibilities and safety concerns. The broad range of vitrification media and devices that are currently available, show differences in their effects on the oocyte ultrastructure and preimplantation development...
August 1, 2017: Cryobiology
https://www.readbyqxmd.com/read/28765301/cloning-mice
#8
Atsuo Ogura
Viable and fertile mice can be generated by somatic nuclear transfer into enucleated oocytes, presumably because the transplanted somatic cell genome becomes reprogrammed by factors in the oocyte. The first somatic cloned offspring of mice were obtained by directly injecting donor nuclei into recipient enucleated oocytes. When this method is used (the so-called Honolulu method of somatic cell nuclear transfer [SCNT]), the donor nuclei readily and completely condense within the enucleated metaphase II-arrested oocytes, which contain high levels of M-phase-promoting factor (MPF)...
August 1, 2017: Cold Spring Harbor Protocols
https://www.readbyqxmd.com/read/28755009/transcriptomic-signature-of-the-follicular-somatic-compartment-surrounding-an-oocyte-with-high-developmental-competence
#9
Satoshi Sugimura, Norio Kobayashi, Hiroaki Okae, Tadayuki Yamanouchi, Hideo Matsuda, Takumi Kojima, Akira Yajima, Yutaka Hashiyada, Masahiro Kaneda, Kan Sato, Kei Imai, Kentaro Tanemura, Takahiro Arima, Robert B Gilchrist
During antral folliculogenesis, developmental competence of prospective oocytes is regulated in large part by the follicular somatic component to prepare the oocyte for the final stage of maturation and subsequent embryo development. The underlying molecular mechanisms are poorly understood. Oocytes reaching the advanced stage of follicular growth by administration of exogenous follicle-stimulating hormone (FSH) possess higher developmental competence than oocytes in FSH-untreated smaller follicles. In this study, the transcriptomic profile of the cumulus cells from cows receiving FSH administration (FSH-priming) was compared, as a model of high oocyte competence, with that from untreated donor cows (control)...
July 28, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28744919/differential-expression-of-insulin-like-growth-factor-family-members-in-immature-cumulus-oocyte-complexes-from-dairy-cows-with-different-genotypes
#10
A C Lopes, M P Palhão, Cac Fernandes, M J Sudano, Acs Castilho, E S Caixeta
It has been evident the improvement of in vitro embryo production (IVEP) in dairy cows. Nevertheless, it is known that differences in the number and quality of oocytes between taurine and zebu females impact the efficiency and economic viability of IVEP. As the insulin-like growth factor (IGF) system is related to follicular and oocyte development, we aimed to quantify mRNA abundance of IGF system members and pregnancy-associated plasma protein-A (PAPPA) in the cumulus-oocyte complexes (COCs) of Gir, 1/2 Holstein × 1/2 Gir and Holstein cows...
July 25, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28743518/nitric-oxide-no-inhibition-of-meiotic-g2-m1-transition-in-anabas-testudineus-oocytes-participation-of-camp-dependent-protein-kinase-pka-in-regulation-of-intra-oocyte-signaling-events
#11
Poulomi Nath, Debabrata Das, Soumojit Pal, Sudipta Maitra
Nitric oxide (NO) regulation of ovarian function in mammals has been studied extensively. However, relatively less information is available on NO action on meiotic G2-M1 transition in teleost oocytes. In the present study using follicle-enclosed oocytes of Anabas testudineus, NO regulation of intra-oocyte signaling events during meiotic G2-M1 transition were examined. Priming with NO donor, sodium nitroprusside (SNP) prevented 17α,20β-dihydroxy-4-pregenen-3-one (17,20β-P)-induced germinal vesicle break down (GVBD) in dose- and duration-dependent manner...
July 22, 2017: Molecular and Cellular Endocrinology
https://www.readbyqxmd.com/read/28735705/impact-of-preimplantation-genetic-screening-on-donor-oocyte-recipient-cycles-in-the-united-states
#12
David H Barad, Sarah K Darmon, Vitaly A Kushnir, David F Albertini, Norbert Gleicher
OBJECTIVE: Our objective was to estimate the contribution of preimplantation genetic screening to in vitro fertilization pregnancy outcomes in donor oocyte-recipient cycles. STUDY DESIGN: This was a retrospective cross-sectional study of US national data from the Society for Assisted Reproductive Technology Clinic Outcome Reporting System between 2005 and 2013. Society for Assisted Reproductive Technology Clinic Outcome Reporting relies on voluntarily annual reports by more than 90% of US in vitro fertilization centers...
July 20, 2017: American Journal of Obstetrics and Gynecology
https://www.readbyqxmd.com/read/28704794/genetic-engineering-as-a-tool-for-the-generation-of-mouse-models-to-understand-disease-phenotypes-and-gene-function
#13
REVIEW
Ann-Kathrin Baumgart, Marc Beyer
The usage of mouse models has been vital for biomedical research over the last decades, yet the generation of these models has been extremely difficult and labor-intensive. The identification and generation of nucleases able to introduce site-specific DNA double-strand breaks, particularly the CRIPSR/Cas system, is a major breakthrough for this field as the endogenous DNA repair machinery can be hijacked to specifically introduce genome modifications at these sites. This allows for the time-efficient and cost-efficient generation of mouse models by delivery of designer nucleases together with donor DNA into fertilized oocytes...
July 10, 2017: Current Opinion in Biotechnology
https://www.readbyqxmd.com/read/28689997/dynamic-reorganization-of-nucleosome-positioning-in-somatic-cells-after-transfer-into-porcine-enucleated-oocytes
#14
Chenyu Tao, Juan Li, Xia Zhang, Baobao Chen, Daming Chi, Yaqiong Zeng, Yingjie Niu, Chengfei Wang, Wei Cheng, Wangjun Wu, Zengxiang Pan, Jinmin Lian, Honglin Liu, Yi-Liang Miao
The nucleosome, the fundamental structural unit of chromatin, is a critical regulator of gene expression. The mechanisms governing changes to nucleosome occupancy and positioning during somatic cell reprogramming remain poorly understood. We established a method for generating genome-wide nucleosome maps of porcine embryonic fibroblasts (PEF), reconstructed 1-cell embryos generated by somatic cell nuclear transfer (SCNT), and fertilized zygotes (FZ) using MNase sequencing with only 1,000 cells. We found that donor PEF chromatin, especially X chromosome, became more open after transfer into porcine oocytes and nucleosome occupancy decreased in promoters but increased in the genic regions...
August 8, 2017: Stem Cell Reports
https://www.readbyqxmd.com/read/28688923/paraoxonase-activities-in-human-follicular-fluid-role-in-follicular-maturation
#15
Susana Meijide, Irantzu Pérez-Ruiz, M Luisa Hernández, Rosaura Navarro, Marcos Ferrando, Zaloa Larreategui, José-Ignacio Ruiz-Sanz, M Begoña Ruiz-Larrea
The paraoxonases (PONs) are antioxidant enzymes associated with beneficial effects against several diseases and some exposures. Little is known, however, about the role of PONs in human reproduction. This work was conducted to investigate whether any association existed between the activities of the PON enzymes (1, 2, and 3) with the follicular size and fertility parameters in assisted reproduction. The study included 100 subfertile women (patients) and 55 proven fertile women (oocyte donors), all undergoing an ovarian stimulation cycle...
June 22, 2017: Reproductive Biomedicine Online
https://www.readbyqxmd.com/read/28687207/high-risk-of-preterm-birth-and-low-birth-weight-after-oocyte-donation-ivf-analysis-of-133-785-live-births
#16
Mohan Shashikant Kamath, Belavendra Antonisamy, Mariano Mascarenhas, Sesh Kamal Sunkara
A higher risk of pregnancy complications occurs after assisted reproductive techniques compared with spontaneously conceived pregnancies. This is attributed to the underlying infertility and assisted reproduction technique procedures involved during treatment. It is a matter of interest whether use of donor oocytes affects perinatal outcomes compared with pregnancies after autologous IVF. Anonymized data were obtained from the Human Fertilization and Embryology Authority. The analysis included 5929 oocyte donation and 127,856 autologous IVF live births...
September 2017: Reproductive Biomedicine Online
https://www.readbyqxmd.com/read/28669480/predictive-factors-for-live-birth-in-donor-oocyte-recipient-cycles
#17
EDITORIAL
Nigel Pereira, Isaac Kligman
No abstract text is available yet for this article.
June 29, 2017: Fertility and Sterility
https://www.readbyqxmd.com/read/28663368/betaine-is-accumulated-via-transient-choline-dehydrogenase-activation-during-mouse-oocyte-meiotic-maturation
#18
Taylor McClatchie, Megan Meredith, Mariame O Ouédraogo, Sandy Slow, Michael Lever, Mellissa R W Mann, Steven H Zeisel, Jacquetta M Trasler, Jay M Baltz
Betaine (N,N,N-trimethylglycine) plays key roles in mouse eggs and preimplantation embryos first in a novel mechanism of cell volume regulation and second as a major methyl donor in blastocysts, but its origin is unknown. Here, we determined that endogenous betaine was present at low levels in germinal vesicle (GV) stage mouse oocytes before ovulation, but reached high levels in the mature, ovulated egg. However, no betaine transport into oocytes was detected during meiotic maturation. Since betaine can be synthesized in mammalian cells via choline dehydrogenase (CHDH; EC1...
June 29, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28662882/ovum-pick-up-interval-in-buffalo-bubalus-bubalis-managed-under-wetland-conditions-in-argentina-effect-on-follicular-population-oocyte-recovery-and-in-vitro-embryo-development
#19
J Konrad, G Clérico, M J Garrido, G Taminelli, M Yuponi, R Yuponi, G Crudeli, M Sansinena
The excellent adaptation of water buffalo (Bubalis bubalis) to swampy environments means that animals are frequently managed in areas with restricted access for reproductive procedures. The objective of this study was to evaluate the effect of the ovum pick-up (OPU) interval on follicular population, oocyte recovery, oocyte quality and in vitro embryo production. Twelve Murrah buffaloes were subjected to two consecutive dominant follicle reductions, and randomly assigned to either 7-day (n=6) or 14-day (n=6) OPU interval groups...
June 20, 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/28658951/qualitative-and-quantitative-analysis-of-proteome-and-peptidome-of-human-follicular-fluid-using-multiple-samples-from-single-donor-with-lc-ms-and-swath-methodology
#20
Aleksandra E Lewandowska, Katarzyna Macur, Paulina Czaplewska, Joanna Liss, Krzysztof Łukaszuk, Stanisław Ołdziej
Human follicular fluid (hFF) is a natural environment of oocyte maturation, and some components of hFF could be used to judge oocyte capability for fertilization and further development. In our pilot small-scale study three samples from four donors (12 samples in total) were analyzed to determine which hFF proteins/peptides could be used to differentiate individual oocytes and which are patient-specific. Ultrafiltration was used to fractionate hFF to high-molecular-weight (HMW) proteome (>10 kDa) and low-molecular-weight (LMW) peptidome (<10 kDa) fractions...
July 10, 2017: Journal of Proteome Research
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